LOCUS       NZ_CP054916            10405 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Streptomyces sp. NA02950 chromosome, complete genome.
ACCESSION   NZ_CP054916
VERSION     NZ_CP054916.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NA02950
  ORGANISM  Streptomyces sp. NA02950
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364155.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 07:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,566
            CDSs (total)                      :: 8,476
            Genes (coding)                    :: 8,147
            CDSs (with protein)               :: 8,147
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 152 of 329
            Pseudo Genes (incomplete)         :: 270 of 329
            Pseudo Genes (internal stop)      :: 20 of 329
            Pseudo Genes (multiple problems)  :: 101 of 329
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:05:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7066485
            Orig. end                         :: 7076890
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054916.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="28"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(278..1078)
                     /locus_tag="HUT19_RS31075"
                     /old_locus_tag="HUT19_31075"
     CDS             complement(278..1078)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533377.1"
                     /locus_tag="HUT19_RS31075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31075"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_176183631.1"
                     /transl_table=11
                     /translation="MSATASGGAAESGAAQGGGPVKSAVRTVELLEFFAGRPGMHSLAA
                     VQEAVGYPKSSLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARP
                     TLDRLSDDTTETIHLARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTALGKALLAT
                     FSDEQVRSMLPETLNPLTENTITDREKLIEELHTIREQGYAVDREENTLGLRCFGVAIP
                     YRTPARDAISCSVPVARLTPTHEQMIKDALFDARDRLTLATRRL"
     gene            1213..2742
                     /locus_tag="HUT19_RS31080"
                     /old_locus_tag="HUT19_31080"
     CDS             1213..2742
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718910.1"
                     /locus_tag="HUT19_RS31080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31080"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_176183632.1"
                     /sec_met_domain="Aldedh (E-value: 1e-39, bitscore: 135.3,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPVWSVDPRTGKQREQVAVEATADEVDRVVSAAHAARGALADR
                     TLRAALLRTAADLLDSSRDLLVEAADAETALGPARLTGELARTTYQLRSFADVVDEGGF
                     LDIRIDHADPAQTPPWPDLRRHKVPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPVV
                     VKAHPDHPVTSELAAATLRRAAERVGLPTELIAVVHGFDAGLDLVRHPLVAAAGFTGSI
                     RGGRALFDAAAARPVPIPFHGELGSLNPVVVTEAAAAERAEQIGAGLAGSMTLGVGQFC
                     TKPGFVLAPAGETGDRLVTALTDAVSNTDPGVLLDHRMRDAFLAGILERAELPEVKAPV
                     TPGAGSEHTVSAGFLTVPARLLAEEGPHDVLLEECFGPVTVVARYEGDADITAVLRRLP
                     GNLTATVQISAAEAEDEGGRAAALLAELTPLAGRVLVNGWPTGVAVAPAQHHGGPYPAT
                     TSTSTSVGGTAVERWLRPVTYQDTPSALLPPELREDNPLGLPRRVDGVRER"
     misc_feature    2755..2768
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.8"
                     /tool="antismash"
     gene            2923..3969
                     /locus_tag="HUT19_RS31085"
                     /old_locus_tag="HUT19_31085"
     CDS             2923..3969
                     /NRPS_PKS="Domain: Aminotran_5 (13-342). E-value: 6.3e-29.
                     Score: 92.9. Matches aSDomain:
                     nrpspksdomains_HUT19_RS31085_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="HUT19_RS31085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31085"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_176183633.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.3e-22, bitscore:
                     76.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAGAEFAPQTTYLNTASSGLIPARSVTAMKAALDEAAAGGPA
                     ADVAFEAVEETRAAYARLVGVPASRVATGGSVAVFAGMIAASLPSGAEVLAAEGDFSSL
                     VNPFAVRRDLKLRTVPLEEIADAVRPGTALVAVSATQSADGRIADLDAVLTAARAHGAR
                     TLVDASQAAGWLPVNAADADYTVSVGFKWLMCPRGVTFLTVPEDLGGLTPVFAGWVAGE
                     KPWDSCYGPVTELAHSARRFDESPSLFSYVAAKHSLTLVEELGVEAIAAHDRALADRFR
                     AGVTELGHRCVAAPGSAIVSVPGLGDAAPRLAERDVYVSARAGNLRASFHLYNTAAEVD
                     RLLAVLAG"
     aSDomain        2962..3948
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT19_RS31085_Aminotran_5.1"
                     /evalue="6.30E-29"
                     /label="HUT19_RS31085_Aminotran_5.1"
                     /locus_tag="HUT19_RS31085"
                     /protein_end="342"
                     /protein_start="13"
                     /score="92.9"
                     /tool="antismash"
                     /translation="TYLNTASSGLIPARSVTAMKAALDEAAAGGPAADVAFEAVEETRA
                     AYARLVGVPASRVATGGSVAVFAGMIAASLPSGAEVLAAEGDFSSLVNPFAVRRDLKLR
                     TVPLEEIADAVRPGTALVAVSATQSADGRIADLDAVLTAARAHGARTLVDASQAAGWLP
                     VNAADADYTVSVGFKWLMCPRGVTFLTVPEDLGGLTPVFAGWVAGEKPWDSCYGPVTEL
                     AHSARRFDESPSLFSYVAAKHSLTLVEELGVEAIAAHDRALADRFRAGVTELGHRCVAA
                     PGSAIVSVPGLGDAAPRLAERDVYVSARAGNLRASFHLYNTAAEVDRL"
     gene            complement(4109..4996)
                     /gene="thpD"
                     /locus_tag="HUT19_RS31090"
                     /old_locus_tag="HUT19_31090"
     CDS             complement(4109..4996)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718912.1"
                     /locus_tag="HUT19_RS31090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31090"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_176183634.1"
                     /sec_met_domain="PhyH (E-value: 9.7e-38, bitscore: 129.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTDLYPTRGTTEVATPRVDPVVWSEPGAPGPIPESELSDFDR
                     DGFLAIEQLITPDEVAVYRGELNRLINDPEVRADARSIIEPKSQDVRSVFEVHRISEVF
                     AKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENFDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALTAF
                     ADKHGIKLFTGKAGSATWFDCNCMHGSGDNITPYSRSNVFIVFNSVENTAVEPFAAPVR
                     RPEYIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="HUT19_RS31095"
                     /old_locus_tag="HUT19_31095"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="HUT19_RS31095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31095"
                     /product="ectoine synthase"
                     /protein_id="WP_176183635.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.9e-53, bitscore:
                     177.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTERHVKAKSGTWESKRIVLARERVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTMRPKTDFR
                     CVCVFNPPITGREDHDENGVYPLITEDEEV"
     misc_feature    complement(5402..5415)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            complement(5447..6712)
                     /gene="ectB"
                     /locus_tag="HUT19_RS31100"
                     /old_locus_tag="HUT19_31100"
     CDS             complement(5447..6712)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-364). E-value: 5.7e-73.
                     Score: 237.8. Matches aSDomain:
                     nrpspksdomains_HUT19_RS31100_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="HUT19_RS31100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31100"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_176183636.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.6e-78, bitscore:
                     261.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNNILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWSDGQMEKQTLARGEQVEQALR
                     AICEEEQTGAQFRGRGLVWGLEFDDKDRASAVCRRAFELGLLVESSGPESEVVKLLPAL
                     TISPEELDEGLRTLARAVRETA"
     aSDomain        complement(5621..6628)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT19_RS31100_Aminotran_3.1"
                     /evalue="5.70E-73"
                     /label="HUT19_RS31100_Aminotran_3.1"
                     /locus_tag="HUT19_RS31100"
                     /protein_end="364"
                     /protein_start="28"
                     /score="237.8"
                     /tool="antismash"
                     /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNNILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWSDGQMEKQTLARGEQVEQALRAICEEEQTGAQFRGRGLVWGLEFD"
     gene            complement(6851..7393)
                     /gene="ectA"
                     /locus_tag="HUT19_RS31105"
                     /old_locus_tag="HUT19_31105"
     CDS             complement(6851..7393)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718915.1"
                     /locus_tag="HUT19_RS31105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31105"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_176183637.1"
                     /transl_table=11
                     /translation="MTAAQADLARSELDLPEGLTLDTPRVEDGAAIWRIARDSKTLDLN
                     SSYSYLLWCRDFAATSVVARDGAGGPVAFITGYIRPERPETLVVWQVAVDHAWRGRGLA
                     ATLLDGLTLRVAGMGVRGIETTITPDNTASNRLFTSFAERHGAPVEREVLFDGGLFPDG
                     GHEPEVLYRIGPLADRP"
     misc_feature    complement(7418..7430)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(7551..7566)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7561..7576
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7561..7578)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.57"
                     /tool="antismash"
     misc_feature    complement(7572..7587)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     gene            7829..9001
                     /locus_tag="HUT19_RS31110"
                     /old_locus_tag="HUT19_31110"
     CDS             7829..9001
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718918.1"
                     /locus_tag="HUT19_RS31110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31110"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_254885873.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1e-17, bitscore:
                     63.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDGAVLHIKGRVLVGPGDEHASGGGGAAGAAGVREAAGATGSAE
                     SVRDELWVVDGKITFQRPAGARDVRLIEGWVLPGLVDAHCHVGLDAHGAVDESTSEKQA
                     LTDREAGALLLRDAGSPADTRWIDDREDLPRIIRAGRHIARTKRYIRNYAHEIEPEDLV
                     AYVAAEARRGDGWVKLVGDWIDRETGDLSACWPRGAVEAAIAEAHRLGARVTAHCFAEE
                     TLAPLVEAGIDCIEHATGLTEETIPLFAERGVAIVPTLVNIATFPRLAQGGETKFPRWA
                     DHMRQLHARRYETVRAAYDAGIPIFTGTDAGGSLAHGLIGQEVAELVKAGIPARDALSA
                     ATWGARAWLGRPGLTEGASADFVVYETDPREDVRVLTSPRRVVLRGRIVA"
     gene            9319..10236
                     /locus_tag="HUT19_RS31115"
                     /old_locus_tag="HUT19_31115"
     CDS             9319..10236
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059801.1"
                     /locus_tag="HUT19_RS31115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_31115"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_176183638.1"
                     /transl_table=11
                     /translation="MPARRLAAATAAAAVSVLGALPAVTAHADTGKTTGKTTGKTTGKA
                     DAVVLRTDLDVALLNKTVSAPLAVALNEVHAPQTASKTALSATLDGVDGGKPFNVLSAD
                     VATARAAVDKQRAEGYSNLVNAKIHVPGLTTMPLIQVQQVTSKAVCAAGKRPTADSNVL
                     GTATVLGKKVTLTAGGTTTVDVPSVGTVRLDLSKTSVTSSTAAATALNLKVSVNPLKLN
                     VSEVNGQITLARATCESPVAADEPKTPEVGNQAKPDPKPENLAETGGSSATPYLIGGGA
                     LLLVMGAGTVTVARRRRAGADADA"
ORIGIN
        1 ccgttgatga cgagcatcga cgtgcgccgg ccgggctcga gcagggtgct cccctcgggg
       61 atgccgaggt gactgcccgc catccaggcc gacttgaggg cgatgtacgg cagaacggag
      121 acgatggcga cggcccgcag gacaggtcgg gaatcggtca tgtccttcag ggtcccgggc
      181 cgccgcccct tcgcgcctcg gcccggccga tgatccgcct ccgccgcgcg gcggaggcgg
      241 ccgtcctccc ggcgggaccg ccgtcctgcc cgcggggcta cagacggcgg gtggcgaggg
      301 tcagccggtc gcgggcgtcg aacagcgcgt ccttgatcat ctgctcatgg gtgggggtca
      361 gccgggccac cggcaccgag cagctgatcg cgtcccgcgc cggggtgcgg taggggatgg
      421 cgacgccgaa gcagcgcagg ccaagggtgt tctcctcacg gtccaccgca tagccctgtt
      481 cgcggatggt gtgcagctcc tcgatgagct tctcgcggtc ggtgatggtg ttctcggtca
      541 gcgggttcag cgtctccggc agcatcgagc gcacctgctc gtcggagaag gtggccagca
      601 gcgccttgcc cagcgcggtg gagtgggcgg gcagccggcg gccgacgcgg gtgaaggggc
      661 gcagatagtg ctgcgactgg cgggtggcga gatagaccac gttggtgccg tcgagccggg
      721 cgaggtggat ggtctcggtg gtgtcgtccg agagccggtc cagggtgggg cgggccgccg
      781 ccaccacctc gtcgccgtcg atgtaggagg tgccgacgag cagcgcgcgc accccgatgc
      841 cgtagcgggt gccggtggcg tcggtctcca cccagcccag ctccaccagg gtgcgcagca
      901 gcatgtacag gctcgacttg gggtagccca cggcctcctg caccgcggcc aggctgtgca
      961 taccggggcg tccggcgaag aactccagca gttcgacggt gcgcaccgcg gacttgaccg
     1021 gacccccgcc ctgggccgcg cccgactcgg cagctccgcc cgacgcggta gcagacatcg
     1081 ccttgacccc tctgttcggc cagccataga gtcccgaggg ggaaattcac catccgggac
     1141 agcgttcagc gtatcgaaca acggtcgaga tgcgagacgc aagcagtgga tacacagcag
     1201 gaggaatccg cggtgtcagc accagtgtgg agcgtcgacc cccgaaccgg aaagcagcgc
     1261 gaacaggtcg ctgtcgaagc caccgcggac gaggtggacc gcgtggtgtc ggccgcccac
     1321 gccgcacgcg gcgccctcgc ggaccgtacg ctgcgcgccg cgctgctgcg caccgccgcc
     1381 gacctcctcg actcctcgcg cgatctgctg gtcgaggccg ccgacgcgga gaccgcgctc
     1441 ggccccgccc ggctcacggg tgaactcgcc cgcaccacct accagctgcg gtccttcgcg
     1501 gacgtggtgg acgagggcgg cttcctcgac atccgcatcg accacgccga ccccgcgcag
     1561 accccgccct ggcccgatct gcgccgccac aaggtcccgc tgggcgtcgt cgccgtctac
     1621 gccgccagca acttcccgct cgccttctcc gtacccggcg gtgacaccgc cagcgccctg
     1681 gccgcgggct gcccggtcgt ggtgaaggcc caccccgacc accccgtgac ctccgaactg
     1741 gccgccgcca ccctgcgccg cgccgcggag cgggtcggac tgcccaccga gctgatcgct
     1801 gtggtgcacg gcttcgacgc gggcctggac ctcgtccggc atccgctggt cgccgccgcg
     1861 ggcttcaccg gctcgatccg gggcggccgc gccctgttcg acgccgcggc cgcccggccg
     1921 gtgccgatcc ccttccacgg cgaactgggc agcctcaacc cggtggtggt caccgaggcc
     1981 gccgccgccg agcgggccga gcagatcggc gcgggcctcg cgggctcgat gaccctgggc
     2041 gtcggccagt tctgcaccaa gcccggcttc gtcctcgcgc ccgcggggga gacgggcgac
     2101 cggctggtca ccgccctcac cgacgcggtc agcaacaccg accccggtgt gctgctcgac
     2161 caccggatgc gcgacgcctt cctcgccggg atcctcgagc gcgccgagct gccggaggtg
     2221 aaggccccgg tcaccccggg cgcgggcagt gagcacacgg tcagcgcggg cttcctgacc
     2281 gtcccggcac gcctcctcgc cgaggagggg ccgcacgacg tcctgctgga ggagtgcttc
     2341 ggcccggtga ccgtggtcgc ccgctacgag ggcgacgccg acatcaccgc cgtcctcagg
     2401 cggctccccg gcaacctcac cgccaccgtc cagatctccg ccgccgaggc cgaggacgaa
     2461 ggcggccggg ccgccgccct cctcgccgag ctgaccccgc tcgcggggcg ggtcctggtc
     2521 aacggctggc cgaccggtgt ggccgtcgcc cccgcccagc accacggcgg cccctacccg
     2581 gcgaccacgt ccacctccac ctccgtcggc ggcaccgccg tcgagcgctg gctgcggccg
     2641 gtcacctacc aggacacgcc gtccgcactg ctgccgccgg agctgcgcga ggacaacccg
     2701 ctcgggctgc cccgccgggt cgacggcgtc cgggagcggt gagaccggtg cgtccggaca
     2761 aacgtccgac cggctgatgt ccacaccccc ggttctgacc cccgcgtccc gtcgcgccgc
     2821 agaaacgtcg caggtcagag gggatccata agggtcactt gaaggcatgc gtcacagatg
     2881 cgcaatggac gcgggggcgg gcccgtccca tagttgatgg acatggagag cctggccggc
     2941 gcggagttcg cgccacagac gacgtatctc aacaccgcgt ccagcggtct gatccccgca
     3001 cgctccgtga ccgccatgaa ggcggccctc gacgaagcgg cggccggagg ccccgccgcc
     3061 gatgtggcct tcgaggccgt cgaggagacc cgcgccgcct acgcccggct ggtcggagta
     3121 ccggcgagcc gggtcgccac cggtggctcg gtcgccgtct tcgccggaat gatcgccgcc
     3181 tcgctgccct ccggcgccga agtcctggcc gccgagggcg acttcagctc cctggtcaac
     3241 cccttcgccg tccgccgcga cctcaagctg cgcaccgtcc cgctggagga gatcgcggac
     3301 gccgtgcggc ccggcaccgc gctggtcgcc gtcagcgcca cccagtcggc ggacggccgg
     3361 atcgccgacc tcgacgccgt ccttacggcg gcccgcgccc atggtgcccg caccctggtg
     3421 gacgccagcc aggccgccgg atggctgccg gtgaacgccg ccgacgccga ctacaccgtg
     3481 tccgtcggct tcaagtggct gatgtgcccg cgcggggtca ccttcctgac cgtgcccgag
     3541 gacctgggcg ggctcacccc ggtcttcgcg ggctgggtcg cgggcgagaa gccctgggac
     3601 agctgctacg ggccggtgac cgagctggcc cactccgccc gccgcttcga cgagagcccc
     3661 agcctgttct cctacgtggc cgccaagcac tccctgacgc tggtcgagga gctgggtgtc
     3721 gaggccatcg ccgcccacga ccgcgcgctc gccgaccgct tccgcgccgg ggtcaccgag
     3781 ctgggccacc ggtgcgtggc cgcgccgggc tcggcgatcg tctcggtgcc ggggctcggg
     3841 gacgcggcgc cccggctggc cgagcgggac gtctacgtct cggcgcgggc cgggaacctg
     3901 cgcgcctcgt tccacctgta caacacggcg gccgaggtgg accggctgct ggcggtgctg
     3961 gcgggctgag cacgccggac gggtgctcgc aaagctgggc acgccggacg gacgcagacg
     4021 agcctgaggg gagccgggac atcccggctc ccctcaggct caggcgcgcg tcaactcacc
     4081 gtacggcggc ggacatcggc cggggacctc accgcaccgg ggtgaagtcc cgcgctccga
     4141 tgtactccgg ccgccggacc ggcgccgcga acggctccac cgcggtgttc tccacgctgt
     4201 tgaacacgat gaagacgttg ctgcgcgagt agggcgtgat gttgtcgccc gagccgtgca
     4261 tgcagttgca gtcgaaccag gtggccgacc cggccttgcc ggtgaacagc ttgatgccgt
     4321 gcttgtccgc gaacgcggtg agcgcctcgt ccgacggggt gcccgcgtcc tgcatctgga
     4381 gcgacttctt gtagttgtcc ttcggcgtcg cacccgcaca gccgaggaag gtgcggtgcg
     4441 agccgggcat gatcatcagc ccgccgttgg tgtcgaagtt ctcggtcagc gcgatcgaga
     4501 cggacacggt ccgcatgttc ggcaggccgt cctcggcgtg ccaggtctcg aagtccgagt
     4561 gccagtagaa gcccgatgcc ccgaagcccg gcttgacgtt gatccggctc tggtggacat
     4621 acacgtccga gcccaggatc tggcgggccc ggcccaccac acgctcatca cgtaccagct
     4681 tcgcgaagac ctcgctgatc cgatggacct cgaacaccga ccgcacgtcc tgcgacttcg
     4741 gctcgatgat cgagcgcgcg tcggcacgca cctccgggtc gttgatcagc cggttcagct
     4801 cgccccggta gaccgcgacc tcgtcgggag tgatcagctg ctcgatcgcg agaaaaccgt
     4861 cgcgatcgaa gtcgctgagc tcggactccg ggatcgggcc cggggctccg ggctcggacc
     4921 acaccaccgg gtccacccgt ggtgtggcca cctcggttgt tcctcgggtc gggtacaggt
     4981 cagtgaccgt ggtcatcgga tcagacctcc tcgtcctctg tgatcagcgg gtatacgccg
     5041 ttctcatcat ggtcctcccg tccggtgatc ggggggttga agacacagac gcagcggaag
     5101 tcggtcttcg gccgcatcgt gtgcttctca tggccgtcca gcaggtacat ggtgccgggc
     5161 gtgatccagt gcttctcgcc ggtctcgtcg ttggtgagct ccgcctcgcc ctcgacgcag
     5221 aggacggcct cgatgtggtt ggcgtaccac atggacgtct cggtgcccgc gtagagcacg
     5281 gtctcgtgca gggagaagcc gacccgctcc ctggccagca cgatgcgctt gctctcccag
     5341 gtgccggact tcgccttcac atggcgttcg gtgccctcga tgtccttgaa ggatcggacg
     5401 atcacggtga gttgctgcct ttctctgtgg tgctgttacg gaacggtcaa gcggtctcgc
     5461 ggaccgcgcg ggcgagggtc cgcaggccct cgtccagctc ctcgggcgag atggtcagcg
     5521 cggggagcag cttgaccacc tcgctctccg ggcccgagct ctcgaccagc aggccgagct
     5581 cgaaggcgcg gcggcacacg gcggaggcgc ggtccttgtc gtcgaactcc aggccccaga
     5641 ccagaccgcg gccgcggaac tgggcgccgg tctgctcttc ctcgcagatg gcgcgcagcg
     5701 cctgctcgac ctgctcgccg cgggccagcg tctgcttctc catctggccg tcggaccagt
     5761 aggtgtccag ggtcgcggcg gcggtgacga acgccgggtt gttgccgcgg aaagtgccgt
     5821 tgtgctcgcc cggctcccag atgtccagct cgggcttgaa caggcacagc gacatcggca
     5881 ggccgtagcc gctgatggac ttcgagacgg tcacgatgtc cggggtgata cccgcctcct
     5941 cgaaggagaa gaaggcaccg gtgcggccgc agcccatctg gatgtcgtcg acgatcagca
     6001 gcatgtcctg gcgctcgcac aggtcggcca gggcacgcag ccactcgggg cgcgccacgt
     6061 tgatgccgcc ctcgccctgg accgtctcga cgatcacggc ggcgggcttg ttgaggccgg
     6121 agccctggtc ctcgagcagc cgctcgaacc acaggaagtc cggaacctgg ccgtcgaagt
     6181 agttgtcgaa cggcatcggg gtgccgtgga ccagcgggat accggcaccg gcgcgcttga
     6241 aggcgttgcc ggtgacggcc agcgagccca gcgacatgcc gtggaaggcg ttggtgaagg
     6301 agacgatcgc ctcgcggccc ttgaccttac gggccagttt cagcgcggac tcgacggcgt
     6361 tggtgcccgt cgggcccggg aacatgacct tgtagggcag gtcacgcggg cggaggatgt
     6421 tgttctggaa cgactccagg aacgcgcgct tggcggtggt ggacatgtcg agtccgtggg
     6481 tcacgccgtc gcgctcgatg tagtcgatca gtgcgcgttt cagcaccgga ttgttgtggc
     6541 cgtagttgag tgacccggca ccggcgaaga agtcgaggta ggtgtggccg tcctcgtcgt
     6601 acatgtggct gccctgggcg cggtcgaaga ccgcgggcca gccgcggcag tagctgcgca
     6661 cctcggactc cagggcctcg aagacgctca gggcgggcgg ggtgatggtc acagcattct
     6721 cctgggagat gagtcttacg aggggggtga ggagggggtg atgggtggga tgaagagttc
     6781 aggggccgcg gtcaggggcc gcggtcgggg cggggccgga tcgacggtga cccggttcgc
     6841 caggaccgga tcagggccgg tccgcgaggg gcccgatgcg gtacagcacc tcgggctcat
     6901 gtcctccgtc ggggaacagt ccgccgtcga agagcacctc ccgctccacc ggcgcaccgt
     6961 gccgttcggc gaacgaggtg aacagccggt tcgaggcggt gttgtccggg gtgatcgtgg
     7021 tctcgatgcc acggactccc atgccggcga cgcgcagagt cagcccgtcc agcagcgtgg
     7081 cggccagtcc gcggccgcgc caggcgtggt cgacggcgac ctgccagacg acgagcgtct
     7141 cggggcgctc ggggcggatg tagccggtga tgaaggcgac cggtccgccc gcgccgtcgc
     7201 gggccaccac ggaggtggct gcgaagtcac ggcaccacag gaggtagctg taggaggagt
     7261 tgaggtccag ggtcttggag tcgcgagcga ttcgccagat cgcggctccg tcctcgacgc
     7321 gaggcgtgtc gagggtcagt ccctccggca aatccaattc gctacgggct aggtctgctt
     7381 gtgcggcggt catgcgaatt aaatttaccg agcggaatcg gaaaatgcat cgtggggagg
     7441 ggttacgcgg aggggggttc atgtgttatc acgcgggcac gtgcgtacgc gcggcggatc
     7501 gctgaaaagc gttgatttgt acgggagtta gtggggcgaa tcgcctgacg tgtggagtcc
     7561 gtcacatctg cgtaaccggc atgtgacggg cgtgaattct gggatccagg ccgtgggaaa
     7621 aatctgggtt taaggtcccg gagacggggc agacgattgt gggaagctgc gctgggaaag
     7681 cgtcacgaat ttatcttttg gcgaccctga attcaatcct ccgcggagaa gtctgctgga
     7741 acgtacaggg aagtcgcaga gaagccgggc ggaacgcaca gggggtaatc ggggcgaggg
     7801 tcgtacccgg tctcgtacag tgcgtgacat gagggatggc gcggtgctgc acatcaaggg
     7861 cagggtcctg gtggggccgg gggacgagca tgcttccggg ggcggcgggg ctgccggggc
     7921 tgccggggtc cgcgaggccg ccggtgccac tgggagtgcc gagagtgtcc gggacgaact
     7981 gtgggtggtc gacggcaaga tcacctttca gcgcccggcc ggcgcccgtg atgtccgcct
     8041 catcgagggc tgggtgctgc ccggcctcgt cgacgcccac tgccatgtcg gcctcgacgc
     8101 ccacggcgcc gtggacgagt ccaccagcga gaagcaggcc ctcaccgacc gtgaggccgg
     8161 ggcgctgctg ctgcgggacg ccggatcgcc cgccgacacc cgctggatcg acgaccgcga
     8221 ggacctgccg cgcatcatcc gcgcgggccg ccacatcgcc cgcaccaagc gctacatccg
     8281 caattacgcg catgagatcg agccggagga cctggtcgcg tatgtcgcgg cggaggcgcg
     8341 ccgcggcgac ggctgggtga agctggtcgg cgactggatc gaccgcgaga ccggggacct
     8401 gtcggcctgc tggccgcgcg gcgcggtcga ggcggccatc gcggaggccc accggctcgg
     8461 cgcccgggtc accgcccact gcttcgccga ggagacgctc gccccgctgg tcgaggccgg
     8521 gatcgactgc atcgagcacg ccaccgggct gaccgaggag accatcccgc tgttcgcgga
     8581 gcgcggggtc gccatcgtcc cgaccctggt caacatcgcc accttccccc ggctcgccca
     8641 gggtggcgag acgaagttcc cgcgctgggc ggaccatatg cggcagctcc acgcgcggcg
     8701 ctacgagacg gtgcgcgcgg cctacgacgc gggcatcccg atcttcaccg gcaccgacgc
     8761 gggcggctcg ctcgcccacg ggctgatcgg ccaggaggtc gccgaactgg tgaaggcggg
     8821 catcccggcc cgggacgctc tctcggcggc cacctggggc gcccgggcct ggctcgggcg
     8881 gccgggcctc acggaggggg cctccgcgga ttttgtggtg tacgagacgg atccgcggga
     8941 agacgtgcgg gtgctgacgt cgccacggcg cgtggtgctg cgcggacgga tcgtggcctg
     9001 acgtacggag aggactggag gctgggggat ccggggccgg ggtgccgaag gggacacgag
     9061 gaacgggagg gacgcggggg acgcgggggc gtaggggtgc gggagagcgc gggagtgccg
     9121 aaggttcgaa ctcagcgcca gaaccccacc cattgggatg aacggacatc atgtgtctgc
     9181 ctgtttaccc tcagtgcgca aatatggcgg agtcgaggcc gtcggcgccc gtggagtccc
     9241 ccgcggcagg cgccggcggc tccatgtctc ctgtgggggt ccactcacgt gtcctgttcc
     9301 cgtaacacct tcttcggcat gcccgctcgc cgtctcgcgg ccgccactgc cgctgccgct
     9361 gtctccgtcc tgggcgccct gcccgcggtc accgcgcacg ccgacaccgg caagaccacc
     9421 ggcaagacca ccggcaagac caccggcaag gcggacgcgg tcgtgctgcg caccgacctg
     9481 gacgtcgcgc tgctcaacaa gacggtctcc gcaccgctcg ccgtcgccct caacgaggtg
     9541 cacgcgcctc agaccgccag caagaccgcg ctctccgcga ccctggacgg ggtcgacggc
     9601 ggcaagccct tcaacgtgct gagcgccgat gtggccaccg cccgcgcggc cgtggacaag
     9661 cagcgggccg agggctactc caacctcgtc aacgccaaga tccatgtgcc cggcctgacc
     9721 acgatgccgc tcatccaggt ccagcaggtg acctcgaagg cggtctgcgc ggccgggaag
     9781 cggccgacgg ccgactccaa cgtgctcggc accgccaccg tgctcggcaa gaaggtcacc
     9841 ctgaccgcgg gcggcaccac cacggtcgac gtgccgtcgg tgggcacggt ccggctcgac
     9901 ctgtcgaaga cctcggtcac ctcgagcacg gcggcggcca cggccctgaa cctcaaggtc
     9961 tcggtcaacc cgctcaagct gaacgtctcc gaggtcaacg gccagatcac gcttgcccgc
    10021 gcgacctgcg agtcccccgt ggccgcggac gagccgaaga ccccggaggt gggcaaccag
    10081 gccaagcccg accccaagcc ggagaacctg gccgagaccg gtggcagctc cgccacgccg
    10141 tacctgatcg gcggcggcgc cctgctgctg gtgatgggcg ccggcaccgt caccgtcgcc
    10201 cgccgccgcc gcgccggagc ggacgccgac gcctgaccga cggcagggcc gcaggccccg
    10261 gcctccgcgc cgtgacgacg gcgcgggggc cggggccgtg ggtcggtggc ccggccgggg
    10321 ccctggatcg ggtgccaggg cctccgtgtc ccgggcgccg ccccggccgg ccggggcctg
    10381 gcggccccgt gctgtggacg gctcc
//
