LOCUS       NZ_CP054916            11320 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Streptomyces sp. NA02950 chromosome, complete genome.
ACCESSION   NZ_CP054916
VERSION     NZ_CP054916.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NA02950
  ORGANISM  Streptomyces sp. NA02950
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364155.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 07:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,566
            CDSs (total)                      :: 8,476
            Genes (coding)                    :: 8,147
            CDSs (with protein)               :: 8,147
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 152 of 329
            Pseudo Genes (incomplete)         :: 270 of 329
            Pseudo Genes (internal stop)      :: 20 of 329
            Pseudo Genes (multiple problems)  :: 101 of 329
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:05:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2377090
            Orig. end                         :: 2388410
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054916.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11320
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11320
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11320
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6320](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6320
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1055..2353
                     /locus_tag="HUT19_RS10030"
                     /old_locus_tag="HUT19_10030"
     CDS             1055..2353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055544.1"
                     /locus_tag="HUT19_RS10030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10030"
                     /product="hypothetical protein"
                     /protein_id="WP_254886144.1"
                     /transl_table=11
                     /translation="MKAWKVLIARSAHGVVGPLGALTAVAVVVALLIALTSRGGAGDAP
                     DGDVPAAAASPRSDRMPKEAKKKKEKYADWDGKVKVLGDGSTSYTGPQPKQLKPRKLKP
                     GEKPPQFVVFSWDGALESDDHLFSRFRQVAKESNATMTFFLSGMYLLPKSKSYLYQPPQ
                     HKRGSAAISFPTDEHIRETLEQLSGAWQEGNEIGSHFNGHFCGAKGGQDWTAEEWKSET
                     EQAYSFVKHWKTNTGFTDIKPLPFDYDVELAGGRAPCLEGQKNLLPVIKSMGWRYDASS
                     PGDFQIWPSKLNGIWNFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPSKHAEWER
                     LTREGYLNGFERVYHGSRAPLFIGNHFEQWNGGIYMQAVEDVMRDVCPRKGVRCVSFKQ
                     LADWLDAQDPEVLAKLRMLDPAESPDWRTFTKK"
     misc_feature    1073..1075
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2470..4449)
                     /locus_tag="HUT19_RS10035"
                     /old_locus_tag="HUT19_10035"
     CDS             complement(2470..4449)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824635.1"
                     /locus_tag="HUT19_RS10035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10035"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_176180125.1"
                     /transl_table=11
                     /translation="MLLRRWAAGSVRLDYPRAGRRGVWRWLPSWRQTSLFFLFCLGIMT
                     GLLSFLYLRTDIPEDLNDFATQQDNVYYWADGSEMARTGPVNRQEVPLEKVPEKVQWAV
                     LAAENASFYSDSGVSPSGLMRAVTRMVTGGETQGGSTITQQYVKNAYLNQRQTFSRKLT
                     EMFMAIKLDDRMSKEEILERYLNTSWFGRGTYGIQRAAYAYYGKDVSKLNPSEGALLAS
                     LLKGAGSYDPTLGEKNRQRVTERWKWVLDRMVDIGKLSPQERAKYTKFPEPKAPPKPEG
                     LTGQVGYLVETARAYVSSHTEISDADFDLGGYQIHTTFEKPKVNALAKAVKKARASFDP
                     KERSGDRHVRIGAASVAPDGRIAALYGGPGYLEQGFNDANASNVPAGTAFTPFVYAAGL
                     RDGVLHDREGPRQPVAPTSLYDGNNKVAVRTPEGPYWDRSGKIVRASNDGDRSYGQVSL
                     RQAIVQSINTPMMQLGMDVGLERVRQASIDAGMLPSSFGARVPAFSLGTATPSSIRMAS
                     AYGTFAAKGVHAPPYSVSGLTRNGEKFRLSRDRAQRAFSEAVANEVDDALRGAVRSEAG
                     SAREAGAAGPDVAGKRGMAQDGTSAWFVGYTKKLSTAVSLSRVDPKTQELLSLEDLGGR
                     GAVPSVDTYPLSIWTRYMTVVGRE"
     misc_feature    complement(4628..4644)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.86"
                     /tool="antismash"
     misc_feature    4699..4715
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.29"
                     /tool="antismash"
     misc_feature    4704..4724
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.64"
                     /tool="antismash"
     gene            5001..6320
                     /locus_tag="HUT19_RS10040"
                     /old_locus_tag="HUT19_10040"
     CDS             5001..6320
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175675.1"
                     /locus_tag="HUT19_RS10040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10040"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_176180126.1"
                     /sec_met_domain="DUF692 (E-value: 5.8e-95, bitscore: 316.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRLGTGIGWRPEIADDIAELPGIDWVEVVAENICPGHVPDALER
                     LRERGTTVVPHGVSLGLGGAEPPDPERLKALGERAEALGAPLVTEHIAFVRAGGPVTAS
                     PLIEAGHLLPVPRTRDALDVLCANVQIAQDALPVPLALENIAALISWPGEEMTEGRFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPTEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAELCARTAPPGVLLERDEDFPAAEELAGELDAIRAVTAAGTAPAIPR
                     TAEATPDAPPMADGPGEAALDAARQRLALGQTSLLSALVAGTPAPEGFDRRRLRVQSKA
                     LTAKRASVIAHVAPELPEILGDGFRPAFVAYADSRPMTGGYRRDALSFAEYLLADDLPR
                     DPAARRRLTQWWKERSGPAPLPVNPAARFARALRHALSGR"
     gene            6326..7366
                     /locus_tag="HUT19_RS10045"
                     /old_locus_tag="HUT19_10045"
     CDS             6326..7366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714418.1"
                     /locus_tag="HUT19_RS10045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10045"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_176180127.1"
                     /transl_table=11
                     /translation="MDTAVALVYVAVAVSAMTLITGTVRSRKAADGPMYAQAHDVLEAA
                     FLAGGPGRVADTVISTMHKDGRLAVGGPGVVSVRQPVARHPVENALLTLCARTPHGGLA
                     LLRRELMRSAEVQAIGDGLARRGLMVRPEAARPWRRWAVAQIVACWLGIFASVWATTNQ
                     DSDSLHELPFIVEVLPPLFLGFIIGAACANAGKHRVTGAGKRALRTYTAQAATTPTTAP
                     VPDHRPGQVGPGDPGPGHHGPYAAQAAAAVAVAVALGGAVALADDQVLRDQLLEAGRAS
                     VGAGGPGGYTGPSSDSSGGSWCGGGGSGCGGSSCGSSSSCGGGGGGSSCGGGSSCGGGG
                     GCGGGG"
     gene            7482..8288
                     /locus_tag="HUT19_RS10050"
                     /old_locus_tag="HUT19_10050"
     CDS             7482..8288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432059.1"
                     /locus_tag="HUT19_RS10050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10050"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_176180128.1"
                     /transl_table=11
                     /translation="MLWVPFLLVAWGAAVISCARLCRAAVAAAQPLDPDPALRSAALGL
                     YETAFLAGGPRRVGDLALVTMYRERRLLLAHTGWVTVVDPEGRNDLERSVISAIGAQGA
                     SPVPAVRTALASAEPVRALAERLVGAGLAVSTDARTNVASAVRQVGAAGVLVLLSAAAA
                     LSLVPPAPGHGPVAAWFGLPLVLTASCLVIARVEAHPYSRWASPAGQQLLGRIDVPPRR
                     AATRHGGGGADDGGDDGELLTAVAVHGTAALPDPALRAALKAHWGQ"
     gene            8389..10206
                     /locus_tag="HUT19_RS10055"
                     /old_locus_tag="HUT19_10055"
     CDS             8389..10206
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (165-549). E-value:
                     2.7e-20. Score: 65.0. Matches aSDomain:
                     nrpspksdomains_HUT19_RS10055_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055539.1"
                     /locus_tag="HUT19_RS10055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10055"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_254885510.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-23, bitscore: 81.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYGILGPLSLSLGMTALLATPATGRPLAAPAPPHTSGTTA
                     GATAAVRARAAVSAGTAAAPPAAAPAWSVSPASPASPVSSALAAAPLGRAAEAQGVALA
                     ARRAAARGIHFGRCPRAEALTAPVTCGTVSVPLDYARPGGPQISLTVSRVRATGPKARR
                     QGALVYNPGGPGASGMAFPSYAPEEEFRRVAAVYDFVGYAPRGVGRSAPLSCQSPTVFA
                     KAPTSSPPHPSAAFKRRMVARAKAYARGCARSAGSALAHYTTLDNARDLDVLRAALGER
                     RLTFIGASYGTYYGAVYATLFPGHVRRMVFDSVVNPDPWQIWYRNNLEQSLAFERRWRD
                     WRGWVARHDDVYHLGTTAERVLASYEKARARLTREPAGAVGTAQLQHAFLRTGYNDDYW
                     DPSARALAAYLRGDPAPLADLAAPDPATAAEDENGNAVYTAVECNDAPWPRTWGTWDRD
                     NTALARRAPFETWDNVAMNLPCAFWPVPHQRPLDIGIGTGTGPGRGTLPPVLLLSAERD
                     AATPYAGAKELWRRLPGSSLVTERNAGTHGLWGGPNACVNRYVDTYLLTGKTPGRSADC
                     AARAEPEPQPEPAPLPDSGKQPAALPGSP"
     aSDomain        8884..10035
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT19_RS10055_Abhydrolase_1.1"
                     /evalue="2.70E-20"
                     /label="HUT19_RS10055_Abhydrolase_1.1"
                     /locus_tag="HUT19_RS10055"
                     /protein_end="549"
                     /protein_start="165"
                     /score="65.0"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGMAFPSYAPEEEFRRVAAVYDFVGYAPRGVGRS
                     APLSCQSPTVFAKAPTSSPPHPSAAFKRRMVARAKAYARGCARSAGSALAHYTTLDNAR
                     DLDVLRAALGERRLTFIGASYGTYYGAVYATLFPGHVRRMVFDSVVNPDPWQIWYRNNL
                     EQSLAFERRWRDWRGWVARHDDVYHLGTTAERVLASYEKARARLTREPAGAVGTAQLQH
                     AFLRTGYNDDYWDPSARALAAYLRGDPAPLADLAAPDPATAAEDENGNAVYTAVECNDA
                     PWPRTWGTWDRDNTALARRAPFETWDNVAMNLPCAFWPVPHQRPLDIGIGTGTGPGRGT
                     LPPVLLLSAERDAATPYAGAKELWRRLPGSSLVTERNAGTHGLW"
     gene            complement(10271..10987)
                     /gene="hemQ"
                     /locus_tag="HUT19_RS10060"
                     /old_locus_tag="HUT19_10060"
     CDS             complement(10271..10987)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="HUT19_RS10060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_10060"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_176180129.1"
                     /transl_table=11
                     /translation="MTAAPEKTPHAGKKAKDLNEVIRYTLWSVFRLRDVLPEDRAGYAD
                     EVEELFAQLAAKDVTVRGVYDLSGLRADADVMIWWHSETSDALQEAYSLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADERARDYVSVYPFVRSYDWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRHVREEVPF
                     FTGRRKEVSELVAGLA"
ORIGIN
        1 cgggttcacc gatcacatcc tgctgaccca gcccgaccac tggccggcgc atatcgcggg
       61 ccatccgccg ggcgcggtgc tcaccttcgt cgggctggac cggctggggc tgggcggcgg
      121 ggggtgggcg ggggcgttct gcatcacggt ggggagttcg gcggcggccg cggtgctggt
      181 cgccctgcgc gcgctgggcg cggaaaaggc ggcgcgcacc gcagcgccgt tcctggtgct
      241 cgccccggcg gcggtgtggg cgggggcctc ggcggacggc tacttcgccg ccgtggcggc
      301 gtgggctctg gcgctgctgg cgctcgcggc ccggcgcacg gtgcgggcac cgggcgccgt
      361 ggcgctggtc tcggggctgc tgctgggcct gacggtgtat ctctcgtacg ggctcaccct
      421 gctggcgctg cccgccctgg ccgtgctggt gctcgcccgt acggtgaagc cgctgccctg
      481 ggtgctggcc ggagtggccg cggtggcggg cgtgttcacg ctgtccgggt tcgtgtggtg
      541 ggaggggtac cacctgctgg tggagcgcta ctaccagggc gtcgccgcgg accggccgta
      601 cgcctactgg gtgtgggcca atctcgccgt cgcgggggtc gccgccggtc cggcctgtgt
      661 ggccggggcg cgccgggtcg tcatggccgc ccccggggca gtacggcggc tgcgcggccg
      721 cggggtgggc gcgccggacg ctctggtggt gctgacctct gccgcgctgc tcgcactggc
      781 cgtcgccgat ctgtccggta tgagcaaggc ggagacggag cgcatctggc tgccgttcgt
      841 cctgtggctg ctgcccggga ccgcgctgct gcccgcgcgg gaccggcgcc actggctcat
      901 ggtccaggcc atggtggcac ttctcatcaa tcatctgctg ctgaccggat ggtgacggga
      961 ccgatgtgaa gagcacgtga tacttctgtg atactggagg ggatgtacat ctgtactggt
     1021 gctgggggcg gacccgttga aggcgctgaa gatcatgaag gcgtggaagg ttttaatcgc
     1081 gcggagcgcc cacggagtgg tggggcccct cggcgcgctc acggccgtgg ccgtcgtggt
     1141 ggccctgctg atagccctga cctcgcgcgg aggcgcgggc gacgcaccgg acggagacgt
     1201 ccccgcggcc gccgcctccc cgaggtcgga ccgtatgccc aaggaggcga aaaagaagaa
     1261 ggaaaaatac gccgactggg acggcaaggt caaggtcctc ggcgacggct ccacttccta
     1321 caccggcccg cagccgaaac agctgaagcc gcggaaactc aagcccggtg agaaaccgcc
     1381 gcagttcgtg gtgttctcgt gggacggggc gctggagagc gacgaccatc tcttctcgcg
     1441 attccgtcag gtcgccaagg agagcaatgc cacgatgacg ttcttcctga gcggtatgta
     1501 tctgctgccc aagtcgaaga gttatctgta ccagcccccg cagcacaagc ggggctcggc
     1561 cgccatatcc ttccccaccg acgagcacat ccgggagacg ctggaacagc tgagcggcgc
     1621 ctggcaggag ggcaacgaga tcggctccca cttcaacggc cacttctgcg gcgccaaggg
     1681 cggccaggac tggaccgcgg aggagtggaa gagcgagacc gagcaggcgt attcgtttgt
     1741 gaagcactgg aagaccaaca ccggtttcac cgacatcaag ccgctgccct tcgactacga
     1801 cgtggaactg gcgggtgggc gtgccccatg tctggagggg cagaagaatc tgctcccggt
     1861 gatcaaatcg atgggctggc gctatgacgc cagttcgccg ggcgacttcc agatctggcc
     1921 gtcgaagctc aacggcatct ggaacttccc gcttcagctg gtgccgtacc cggagagcga
     1981 gaagcaggtc ctgtccatgg acttcaactt cctcttcaac cagtccgggg acagcaccga
     2041 gggcgatccg tcgaaacacg cggagtggga gcggctgacc cgcgagggct atctgaacgg
     2101 cttcgagcgc gtctaccacg gcagccgcgc cccgctgttc atcgggaacc acttcgagca
     2161 gtggaacggc ggcatctaca tgcaggccgt cgaggacgtg atgcgggacg tctgccctcg
     2221 taagggggtg cggtgtgtgt cgttcaaaca gctcgccgac tggctggacg cccaggaccc
     2281 ggaggtgctg gccaagctgc gcatgctgga tccggcggaa tccccggact ggcggacctt
     2341 caccaagaaa tagcgccggt ccggcgcggc ctctcctcca tcacccccgg gacattcccc
     2401 ccgggggtga tgccgttccc gtttcacaac cgggtgtgtt tcattcccgg ctgtttattc
     2461 ccggctgtct cattcccggc cgaccaccgt catatagcgc gtccagatgg acagcggata
     2521 ggtgtccacc gaggggaccg cgccccttcc gcccaggtcc tccagcgaca gcagctcctg
     2581 ggtcttgggg tccacccgcg acagcgacac cgccgtggac agcttcttgg tgtagccgac
     2641 gaaccaggcc gaggtgccgt cctgcgccat accgcgcttt ccggccacat cgggtccggc
     2701 ggcaccggcc tcgcgcgccg aacccgcctc gctccggacg gcgccgcgca gcgcgtcgtc
     2761 cacctcgttc gcgaccgcct cgctgaacgc ccgctgggcg cggtccctgc tcagccggaa
     2821 cttctccccg ttacgggtga gcccgctgac cgagtacggc ggggcgtgta cgcccttggc
     2881 ggcgaacgtc ccgtaggcgc tggccatccg gatggagctg ggagtggcgg tgccgagcga
     2941 gaacgcgggc acccgggcgc cgaagctgct gggcagcatc cccgcgtcga tggaggcctg
     3001 ccggacccgc tccaggccga cgtccatgcc gagttgcatc atcggggtgt tgatcgactg
     3061 cacgatggcc tggcgcagac tgacctgccc gtaggacctg tcgccgtcgt tgctcgcccg
     3121 cacgatcttc ccgctgcggt cccagtacgg gccttccggc gtccgcacgg ccaccttgtt
     3181 gttgccgtcg tagaggctcg tgggggccac cggctgccgc gggccctcgc ggtcgtgcag
     3241 caccccgtca cgcagcccgg cggcgtagac gaacggggtg aaagccgtac cggcgggcac
     3301 attggaggcg ttggcgtcgt tgaacccctg ctccagatag cccgggccgc cgtacagcgc
     3361 ggcgatcctg ccgtccggtg cgacggaggc ggcaccgatc cgcacatggc ggtcgccgga
     3421 ccgttccttg gggtcgaacg aggcgcgcgc cttcttcacc gccttggcca gcgcgttcac
     3481 cttcggcttc tcgaaggtgg tgtggatctg gtaaccaccg aggtcgaagt cggcgtcgga
     3541 gatctcggtg tgagagctga cgtacgcccg ggcggtctcc accagataac cgacctgtcc
     3601 ggtcagcccc tcgggtttgg gcggcgcctt gggctcgggg aacttcgtgt acttcgcgcg
     3661 ctcttggggt gagagctttc cgatgtcgac catccggtcg agcacccact tccagcgctc
     3721 cgtcacccgc tggcggttct tctcgccgag cgtggggtcg tatgacccgg ctcccttgag
     3781 cagtgaggcg agcagcgcgc cctcgctcgg attcagcttc gagacgtcct tgccgtagta
     3841 cgcgtaggcg gcccgctgaa taccgtaggt gccacggccg aaccagctgg tgttcagata
     3901 ccgctcgaga atctcctcct tgctcatccg gtcgtccagc ttgatcgcca tgaacatctc
     3961 ggtgagcttg cgggagaacg tctggcgctg attcagatag gcgttcttca cgtactgctg
     4021 cgtgatggtg gaaccgccct gggtttcccc gccggtcacc atccgggtca cggcgcgcat
     4081 cagcccgctg ggcgagaccc cgctgtcgga gtagaaactg gcgttctcgg cggccagaac
     4141 ggcccactgg accttttccg gcaccttctc gagggggacc tcctggcggt tgaccggtcc
     4201 ggtgcgggcc atctcgctgc cgtcggccca gtagtagaca ttgtcctgct gggtggcgaa
     4261 gtcgttcagg tcctcgggta tgtccgtccg caaatagagg aagctcagca gcccggtcat
     4321 gatgcccagg cagaagagga agaacaggga cgtctgacgc catgacggca gccagcgcca
     4381 cacccccctt ctgcccgccc ggggatagtc cagccggaca ctgccggccg cccagcggcg
     4441 cagcagcatg agcctgcgcc ccgagggcgt cgtcgaacgg cggcggcccc ccgggggaac
     4501 ccgaccgcgc cggaccctcg gaggagcggc acggcggtgc ctccggtgct tccccgccgc
     4561 gttgctgtcg ctgctgccct gcacttcttt cccccacgct cactccgcgc gcgggcgcgg
     4621 cacaaagatg tgatcttcca tccccctcac gaccacacga tcctattttc ccctcctgac
     4681 acctgtcgcc gcaggggccc aaaattttgt aacagtgacg aaattccaca actcgggacg
     4741 gccggtgacc attcgccccc gccggacacc cgccgccaca cgggccccgg aggggaggag
     4801 gggcggccgt gcggtccgcc cccgatcccg ccccgggacc gtccccgagc ccccggggcc
     4861 cctcccggag tgaacctccg aagctcaaat gaacctctga accggacggc cgggggattc
     4921 gtcctcttcc tgtggggtca tgaccccggc acgcgagaac acaccgcgcg gaacgcggta
     4981 cggaacgagg agggggagcc atggtgcgcc tcggtaccgg aatcggctgg cgaccggaaa
     5041 tcgccgatga catcgccgaa ctgcccggca tcgactgggt ggaggtggtc gcggagaaca
     5101 tctgccccgg ccatgtgccc gacgcgctgg agcggctgcg ggagcgcggc accaccgtgg
     5161 tgccgcacgg cgtctccctg ggcctgggcg gtgccgaacc gcccgacccc gagcggctca
     5221 aggccctcgg cgagcgggcg gaggcgctgg gcgcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cggggggccg gtcaccgcgt cccccctgat cgaggcgggg catctgctgc
     5341 cggtgccccg cacccgggac gcgctcgacg tgctgtgcgc gaacgtacaa atcgcgcagg
     5401 acgcgctgcc ggtgccgctc gcgctggaga acatcgcggc gctgatctcc tggcccggcg
     5461 aggagatgac ggaggggcgg tttctggccg agttggtgga gcggaccggg gtgcggctgc
     5521 tgatcgacgt cgccaatctg cacaccaacc acgtcaaccg gggcgaggac ccggccaagg
     5581 ccctggacga gctgccgacc gaggccatcg cctacgtcca tgtggcgggc ggtgtcgaac
     5641 gggacggggt gtggcatgac agccatgccc atccggtgac ccagccggtg ctcgacgtgc
     5701 tcgccgagct gtgcgcgcgg acggcaccgc ccggcgtact gctggagcgc gacgaggact
     5761 tcccggcggc cgaggagctg gcgggcgaac tcgacgcgat ccgcgcagtg acagcggcgg
     5821 ggacggcccc cgcgatcccg cggaccgctg aggcgacccc ggacgctccc ccgatggccg
     5881 acggccccgg cgaggccgcg ctcgacgccg cccggcagcg gctcgcgctc ggccagacct
     5941 ccctgctgtc cgcgctcgtc gcgggcaccc cggcccccga aggcttcgac cggcggcggc
     6001 tgcgcgtcca gagcaaggcg ctgacggcca agcgggcctc ggtgatcgcc catgtggcgc
     6061 ccgagctgcc ggagatcctg ggcgacggtt tccgcccggc cttcgtggcc tacgcggaca
     6121 gccgcccgat gaccggcggc taccgccgcg acgcgctgtc gttcgccgag tatctgctcg
     6181 ccgacgatct gccccgggac ccggcggcac ggcggcggct gacccagtgg tggaaggagc
     6241 ggtcggggcc cgccccgctg ccggtgaacc ccgcggcgcg cttcgcccgc gccctgcgcc
     6301 acgcgctgag cggaaggtga gagacatgga tacggcagtg gctctggtct atgtggcggt
     6361 cgcggtctcc gcgatgaccc tcatcacggg caccgtccgc tcccgcaagg cggccgacgg
     6421 cccgatgtac gcccaggcgc acgacgtcct ggaggccgcg ttcctggcgg gcgggcccgg
     6481 ccgggtggcg gacaccgtga tcagcacgat gcacaaggac ggacggctgg cggtcggcgg
     6541 ccccggggtg gtctcggtac ggcagccggt cgcgcgccac cccgtggaga acgcgctgct
     6601 gacgctctgc gcccgcaccc cgcacggcgg actggccctg ctgcgccggg agctgatgcg
     6661 cagtgcggag gtgcaggcga tcggcgacgg gctggcacgg cgcgggctga tggtgcggcc
     6721 cgaggccgcc cgcccctggc ggcgctgggc ggtggcgcag atcgtggcct gctggctggg
     6781 gatcttcgcg agcgtctggg cgacgacgaa ccaggacagc gacagcttgc acgagctgcc
     6841 gttcatcgtc gaggtcctcc ccccgctgtt cctcggcttc atcatcggcg cggcctgcgc
     6901 gaacgcgggc aagcaccggg tgaccggtgc cgggaagcgg gcgctgcgga cgtacaccgc
     6961 gcaggccgcg accactccca cgaccgcccc cgtgccggac caccgcccgg ggcaggtcgg
     7021 accgggggac cccggaccgg gtcatcacgg tccgtacgcc gcccaggccg cggccgccgt
     7081 ggcggtcgcc gtcgcgctgg gcggcgcggt ggccctggcc gacgatcagg tgctgcggga
     7141 ccagctgctg gaggcgggca gggcgtcggt cggcgcgggg ggccccggcg ggtacaccgg
     7201 cccctcctcg gactcctccg gcggctcgtg gtgcggcgga ggcggttccg gctgcggtgg
     7261 gtcctcctgc ggcagcagct cgtcgtgcgg aggcgggggc ggcggatcga gctgcggtgg
     7321 cggttccagc tgcggcggag gcggtggctg cggcggaggg ggctgagcca cccggaacgg
     7381 gcgcgctgag gacaccacgg cacgtggagc ccataccgcc cgcgccgttc cacatcgtga
     7441 aaactgtggc gcggacgggc ccggtccagt agaactcggc catgttgtgg gtcccgttcc
     7501 tgctcgtcgc ctggggtgcc gcggttatca gctgcgcccg gctgtgccgg gccgcggtcg
     7561 ccgccgccca gcccctggac cccgacccgg ccctgcgctc ggcggcgttg ggcctgtacg
     7621 agacggcctt tctggcgggc ggcccgcgcc gggtcggcga tctcgcgctg gtgaccatgt
     7681 accgcgagcg ccgtctgctg ctcgcccaca ccggctgggt cacggtggtg gaccccgagg
     7741 gccggaacga tctggagcgc tcggtgatct ccgcgatcgg tgcgcagggc gcgtcgcccg
     7801 taccggccgt ccggaccgcc ctcgcctccg ccgagccggt acgggccctg gccgagcggc
     7861 tggtgggcgc ggggctcgcg gtgtccaccg acgcgcggac gaacgtggcg tccgcggtac
     7921 ggcaggtcgg ggcggcgggg gtgctggtcc tgctgtcggc ggcggccgcg ctgtcgctgg
     7981 tgccgcccgc gccggggcac gggccggtgg cggcgtggtt cggactgccg ctggtcctca
     8041 ccgcgagctg tctggtgatc gcccgggtcg aggcgcatcc gtactcgcgc tgggcgtccc
     8101 cggccggaca gcagctgctg ggccggatcg acgtaccccc gcggcgggcc gcgacccggc
     8161 acggcggggg cggggccgac gacggaggtg acgacggcga gctgctcacc gcggtcgcgg
     8221 tccacggcac cgccgcgctc cccgaccccg cgctgcgcgc ggcactgaag gcgcattggg
     8281 gtcaatgacc ccgacaccgc cgcgcggtgc tttacacagc caacaaccgc acgaaatatg
     8341 ccttttatcg cgtcacgcat tttcgcgcac cgcggaaagg gatggcttgt gagagcagca
     8401 gcgctgtacg gcatcctcgg accgctttcc ctgtcgctcg gtatgaccgc gctgctggcc
     8461 accccggcca ccggccgtcc gctggccgcg cccgccccac cgcacaccag cggcaccacg
     8521 gccggtgcca ccgccgccgt gcgcgcccgg gccgccgtgt ccgccgggac cgccgccgct
     8581 ccgccggccg ccgctcccgc ctggtccgtc tctcccgcct cccccgcctc ccccgtctcc
     8641 tccgcgctgg ccgcagctcc cctcggccgg gccgccgagg cccagggcgt ggcgctcgcc
     8701 gcccggcggg ccgccgcccg cgggatccac ttcggccgct gcccccgtgc cgaggcactg
     8761 accgcccccg tcacctgcgg cacggtctcc gtcccgctgg actacgcgcg gcccggcggc
     8821 ccgcagatct ccctcaccgt cagccgggtg cgtgccaccg gccccaaggc ccggcgccag
     8881 ggggcgctgg tctacaaccc gggcggaccc ggcgcctccg ggatggcgtt cccctcgtac
     8941 gcgcccgagg aggagttccg gcgagtggcg gcggtgtacg acttcgtcgg ctacgcaccc
     9001 cgcggagtcg gccgctccgc accgctgtcc tgccaatcgc ccacggtctt cgccaaggct
     9061 cccaccagct ccccgcccca tccgtcggcc gccttcaaac ggcgcatggt ggcccgggcc
     9121 aaggcgtacg cgcggggctg cgcccgcagc gcggggtccg cgctcgcgca ctacaccacc
     9181 ctggacaacg cccgtgacct cgacgtgctg cgcgccgcgc tgggcgagcg gcggctgacc
     9241 ttcatcggcg cctcgtacgg cacgtactac ggcgcggtgt acgccacgct cttccccggc
     9301 catgtacgcc ggatggtctt cgacagcgtg gtcaaccccg acccctggca gatctggtac
     9361 cgcaacaacc tcgagcagtc gctcgccttc gagcgccgct ggcgggactg gcgcggctgg
     9421 gtggcccgcc atgacgacgt ctaccacctc ggcaccaccg cggagcgggt gctcgcctcg
     9481 tacgagaagg cgcgggcacg gctgacgcgg gagcccgcgg gcgcggtggg caccgcccag
     9541 ctccaacacg cgttcctgcg caccggctac aacgacgact actgggaccc gagcgcccgg
     9601 gcgctggccg cctatctgcg cggcgacccc gccccgctgg ccgacctggc cgcgccggac
     9661 cccgccacgg ccgcggaaga cgagaacggc aacgccgtct acacggccgt ggagtgcaat
     9721 gacgccccct ggccgcgcac ctggggcacc tgggaccgcg acaacaccgc cctcgcccgc
     9781 cgcgcgccct tcgagacctg ggacaacgtc gcgatgaacc tgccgtgcgc gttctggccg
     9841 gtgccgcacc agcgcccgct cgacatcggc atcggtacgg gcaccgggcc gggccgcggc
     9901 accctgccgc cggtgctgct gctgtccgcc gaacgcgacg cggccacgcc gtacgcgggg
     9961 gcgaaggagc tgtggcggcg gctgcccggc tcctcactgg tgaccgagcg gaacgcgggg
    10021 acgcacgggc tgtggggcgg ccccaacgca tgcgtcaacc ggtatgtcga cacctatctg
    10081 ctgaccggga agacccccgg ccggagcgcc gactgcgccg cccgcgcgga gccggaaccg
    10141 cagccggaac cggccccgct gcccgactcc ggcaagcagc ccgccgcact gcccggctcc
    10201 ccgtgagggc ctgagacccc ggacgcccgg cacccccgcg ggtgccgggc gtccggcgtc
    10261 ggccgcgccg tcaggcgagt ccggcgacca gctcggagac ctccttgcgc cgtccggtga
    10321 agaacggcac ctcctcgcgc acatggcggc gcgcctcgga gccgcgcaga tggcgcatga
    10381 ggtcgacgat ccggtgcagt tcgtccgcct cgaaggcgag gatccactcg tagtcgccga
    10441 gcgagaagga ggcgacggtg ttggcgcgca cgtccgggta gccgcgcgcc atcttgccgt
    10501 ggtcggcgag catcgcgcgg cgctcctcgt ccggcagcag gtaccagtcg taggaccgca
    10561 cgaacgggta gacgctgacg tagtcacgcg cccgctcgtc cgcgaggaag gcggggatgt
    10621 gcgacttgtt gaactccgcc gggcggtgca gcgccatgtt cgaccacacc ggctccaggg
    10681 cccggcccag ccgggtgcgg cggaagaggc tgtacgcctc ctggagggcg tccgaggtct
    10741 ccgagtgcca ccagatcatg acgtccgcgt cggcccgcag cccggacagg tcgtagacgc
    10801 cgcgcacggt gacgtccttg gcggcgagct gggcgaagag ctcctcgacc tcgtcggcgt
    10861 agccggcgcg gtcctccggc agcacatcac gcagccggaa caccgaccac agggtgtagc
    10921 ggatgacctc gttgaggtcc ttggccttct tgccggcgtg gggagtcttc tcaggggcag
    10981 cagtcatgct ccctattctc gctcccggcc tccggtgccc ggctccaggg tggctccggt
    11041 gtgctccacc agctcgtcgg ccgcccggcg ggcgccggcg atacaggcgg ggatgccgac
    11101 gccgtcgtac tgagcgccgc acacccgcag tcccggcagc ttggcgacct ccgcgcggat
    11161 gcgggccacc cggtccagat ggccgacggg gtactgcggc agcccgccgt cccagcggtc
    11221 gacccgggtc gcgacgggcc gggcggcgag acccaccgcc tcaccgagat cggcgagcga
    11281 catccctacc agatcggcgt cgtcgcgctt caggtcctcc
//
