LOCUS       NZ_CP054916            10933 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Streptomyces sp. NA02950 chromosome, complete genome.
ACCESSION   NZ_CP054916
VERSION     NZ_CP054916.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NA02950
  ORGANISM  Streptomyces sp. NA02950
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9619720)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364155.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 07:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,566
            CDSs (total)                      :: 8,476
            Genes (coding)                    :: 8,147
            CDSs (with protein)               :: 8,147
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 152 of 329
            Pseudo Genes (incomplete)         :: 270 of 329
            Pseudo Genes (internal stop)      :: 20 of 329
            Pseudo Genes (multiple problems)  :: 101 of 329
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:05:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1198999
            Orig. end                         :: 1209932
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054916.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="7"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10933
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5933](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5933)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            642..2153
                     /locus_tag="HUT19_RS04835"
                     /old_locus_tag="HUT19_04835"
     CDS             642..2153
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="HUT19_RS04835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04835"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_176179241.1"
                     /transl_table=11
                     /translation="MITAKPRLACIDPPRLLAGLDEAYRLDRVGHLTVHGQMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKLTSVIESAARRDGRCAVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGAMLAAEALGAEDVCIAVHRPDVEESLATAIAERAPSGPRLRVSRTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRTSDSGLGGLPTLLSNTETFAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVAGKFVMETPTGVPLTYILELCNLTPGQGVLVGGYHGKWLDPNSIH
                     ATTISRDSMKKYGARLGAGAVLPIPEDTCPLGETARVARWLAAETAGQCGPCFIGLPAL
                     ADALGQVERGGGQTAIDSVRAFINSVKKRGACSHPDGTAGFVTTALDAFPEEIESHALG
                     MGCGRPTLGVLPLPEDSKPLALPPGPSAADPRREREPRGRMEQLIVDWSLCQGHGLCAD
                     IIPDVLELGMDGYPASAKMDVPRQFRAQAVRAVRRCPALALRIEE"
     gene            2352..3347
                     /locus_tag="HUT19_RS04840"
                     /old_locus_tag="HUT19_04840"
     CDS             2352..3347
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181497.1"
                     /locus_tag="HUT19_RS04840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04840"
                     /product="SCO0930 family lipoprotein"
                     /protein_id="WP_176179242.1"
                     /transl_table=11
                     /translation="MEKRRQIAFAGVSVAMVLLTAACGSSKDNSSGQSSNVQPAGGAQN
                     VGSGSTPGAAAGSGAAGGYQAGGDTGAAAGSGDTGQSGAAKEVTAKKDPKLGEIVTDAK
                     GWTLYRFDEDTPKPAKSNCNDACATKWPPVPADDATAGTGIDKSKLGSVTRSDGTKQLT
                     LEGWPVYRYAGDTKAGDTKGHGVGGTWNALAPNGKPAGKKATGADESLQLMLNNNAELG
                     KIIVDGKGMTVYRFNKDSAWPMKTGCLGACLDTWKPVKAVNTSKVAGLDASKVTSFTRP
                     DGKKQAAFDCWLLYTFTGDTKPGDTNGQGKAGQWFAVTDKGKKAGVAAAQ"
     gene            3432..3962
                     /locus_tag="HUT19_RS04845"
                     /old_locus_tag="HUT19_04845"
     CDS             3432..3962
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056768.1"
                     /locus_tag="HUT19_RS04845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04845"
                     /product="hypothetical protein"
                     /protein_id="WP_176179243.1"
                     /transl_table=11
                     /translation="MSPALRATALCAALLFLSGCAGNSTAHDATKHPREKAAPSAKEFG
                     KAGKPAPLAGIADAIGCKAKVITEVDELRQGSCTSGKNRYTMLTFATEQGQHDWLSVSK
                     DYGGTYLVGKRWSVTGLSTGSLSSLREKIGGDIEHGMSMSHGGSGGSHEGSGSKGGSGS
                     HDGSTSHDGMDGM"
     gene            complement(4001..4678)
                     /locus_tag="HUT19_RS04850"
                     /old_locus_tag="HUT19_04850"
     CDS             complement(4001..4678)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="HUT19_RS04850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04850"
                     /product="HAD family phosphatase"
                     /protein_id="WP_176179244.1"
                     /sec_met_domain="HAD_2 (E-value: 7.7e-23, bitscore: 80.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEKLLAWTPAAIVFDCDGTLMDSERHWEEARDMVLREHGTAPT
                     EEFARRTKGLHYSECGRMLAEFAGHPELAGQMTGQLLDAFRTLVAEHPVTMPGAPELVE
                     KAAAFAPLAIASNCPRDVVEDGLAKAGLLRHFGHVLVPEGEMRPKPYPDVYLEAARLCG
                     AAPGDALAVEDSHCGFLSASRAGLRVLGVGPRKPADERPPVDLWVDTLADPDLVRWAET
                     RVA"
     misc_feature    4801..4816
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     misc_feature    4863..4883
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(5001..5933)
                     /locus_tag="HUT19_RS04855"
                     /old_locus_tag="HUT19_04855"
     CDS             complement(5001..5933)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056766.1"
                     /locus_tag="HUT19_RS04855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04855"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_176179245.1"
                     /sec_met_domain="AfsA (E-value: 4e-19, bitscore: 67.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVREACGLSEPSGPTGRPLSWSRTVEREAVHRVSVAEVLLTDVR
                     TLGDDRFEAAAQWPRSHPTFPHGGDGRHHPLMIAETLRELGIYLPTRYYAVPAGAHFLI
                     RDLFYRLDPEAEPRAPHGATDITCHAAVTDLRTTPSGRFVSGMRLDIALSASGRRFAWA
                     GGTARFLDGTSYAETRSAASGQTARRRLRAAVRPSPGQLAVPAARDVLVVRDGGVVYLD
                     PADPRHPFFYDHWSDHVPGLVLLESARQAAALATDGTLTRPVACRLKAIRFTESYPPAV
                     VECTSHGRTCVFRLRQAEMCTAVGVLQYL"
     misc_feature    complement(5935..5948)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 19.06"
                     /tool="antismash"
     misc_feature    5936..5949
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(5941..5954)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.63"
                     /tool="antismash"
     misc_feature    5947..5956
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     gene            6025..6717
                     /locus_tag="HUT19_RS04860"
                     /old_locus_tag="HUT19_04860"
     CDS             6025..6717
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712845.1"
                     /locus_tag="HUT19_RS04860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04860"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_254885442.1"
                     /transl_table=11
                     /translation="MKDGEHVQERAEQTRRSLLEAAASLFDERGYAGTSISDITHRSGH
                     TSGAMYFHYTSKERLALAVVEEHFATWPALIEQHTAIDAPALEQLVRLSFAVARAFRDD
                     IVVRAGARLWTERTLIEAPLPPPFVGWIDAVGKMLEQAQADGELAPHVDPQAAARTVVC
                     AFFGLHTVSEALDGRRLVEDHLTDLWTLLLPSLQARPGATAELLAAARGTDPVPLRADD
                     EAVGTDEM"
     gene            complement(6727..7536)
                     /locus_tag="HUT19_RS04865"
                     /old_locus_tag="HUT19_04865"
     CDS             complement(6727..7536)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058037.1"
                     /locus_tag="HUT19_RS04865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04865"
                     /product="endonuclease I family protein"
                     /protein_id="WP_176179246.1"
                     /transl_table=11
                     /translation="MHVTRVNRWKSLALLAGVATLAVTAPAPATAEVTATSTPYDDTYY
                     KDAIGKTGDPLKDALHTIISTDVTRLSYDEVWDALKKTDEDPDNSAHVILLYTGTSQSK
                     NANGSGPDQWNREHVWAKSHGDFGTATGPGTDVHHLRPANVTVNSIRGNKDFDAGGSEV
                     EGAPGNRTDDDSFEPRDQVKGDVARMVLYMSVRYEGDDDFPDLEVNDTAGNGSRPNLGR
                     LSVLKKWNDEDPPDTFEKHRNDVIYDTIQHNRNPFVDHPEWVGEIWK"
     gene            complement(7589..8149)
                     /locus_tag="HUT19_RS04870"
                     /old_locus_tag="HUT19_04870"
     CDS             complement(7589..8149)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HUT19_RS04870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HUT19_04870"
                     /product="hypothetical protein"
                     /protein_id="WP_176179247.1"
                     /transl_table=11
                     /translation="MGVLYGIVRVLSQTLVPDGAIQAAAGILRDRAALTTRLGASDRAA
                     QRITRAWPTSPSSAPRSPRQRLREQQAEPTGRAVDFVSGLRGLRGIGGEAAAQGPHAQL
                     LADSHDRGPRAHWSRTPVTGGGSPNWARTRSWCVGRGLRRALGVLARPVLIGGAKGAVD
                     HSRLVMALTQNLCGLFTIVRSCV"
     misc_feature    8289..8298
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.26"
                     /tool="antismash"
     misc_feature    8893..8906
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.6"
                     /tool="antismash"
     gene            <8941..9234
                     /locus_tag="HUT19_RS04875"
                     /old_locus_tag="HUT19_04875"
                     /pseudo=""
     CDS             <8941..9234
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942562.1"
                     /locus_tag="HUT19_RS04875"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04875"
                     /product="DUF1348 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="SEWRNRDVFLRGRSEIVEFLTAKWRRELGYRLRKELWAWAGNRIA
                     VRFEYEHHDAAGRWFRAYGNENWEFDENGLMRKRYASINDLPITAEERRIAV"
     gene            9283..10266
                     /locus_tag="HUT19_RS04880"
                     /old_locus_tag="HUT19_04880"
     CDS             9283..10266
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056751.1"
                     /locus_tag="HUT19_RS04880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04880"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_254885443.1"
                     /transl_table=11
                     /translation="MEKQMSHGTAPARPVLEPAAAELAEASAKPPFLYQMSPAEGRRAV
                     DEAQSGPVPKPEVDEEWISVTGIPEGGVKARILRPPGVGGTLPVILYIHGAGWVFGNAH
                     THDRLVRELAVRVGAAVVFPEYDLSPEVHYPVALEQNYAVGQWIVAHGEGAGLDPARIA
                     VTGDSVGGNMSAALTLLAKERGDLSLVQQVLLYPVTDAAFDTGSYEQFADGYYLTREGM
                     RWFWDQYTTDPAQRAEITASPLRADPAQLAGLPPALVITGEADVLRDEGEAYAARLRAA
                     GVPVTAVRMQGIIHDFLMLDALRDTNAARAALALAVDTLRNALHPA"
     gene            complement(10282..10869)
                     /locus_tag="HUT19_RS04885"
                     /old_locus_tag="HUT19_04885"
     CDS             complement(10282..10869)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991358.1"
                     /locus_tag="HUT19_RS04885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT19_04885"
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="WP_176179248.1"
                     /transl_table=11
                     /translation="MHATLRLAQQPEADALLGRSPLALMIGMLLDQQVPMEWAFAGPYT
                     IAQRMGSDELDAQEIAAQDPERFVELLSQKPAVHRYPGAMAKRIQQLCQYLVEHYDGDA
                     EAVWRDADSGAELLKRLNALPGYGKQKAQIFLALVGKRLGAGPEGWREAAGGYGEEGAY
                     RSVADITGPESLDKVRAHKQEAKRQAKRSAGK"
ORIGIN
        1 accgcggcgg tgctgtgggg gctcgcggcc accgaccaac tgctgctgca accgaaccac
       61 cggctgatcg cccagggggt ccaccgggcc gccgccatcg ggggtttgtg cttcctcgcc
      121 ctccatgtct gggtgaaggt ggcggagagc cacacgaccg cggcgaccat cgtgataccg
      181 ttcgctgacg caaatcggcc tgtgctcatc ggccttggaa ccatcgccgc ctacctcttc
      241 accatggcgg cggtgaccgg ggcggtgcgc agtgcctttg cctccgccgg caagtcccgc
      301 tggcggtacc gttggtggcg ggcgctgcat gtggccgcgt atccggcgtg gtgcgccgca
      361 ctgatccacg gcctcaaggc aggccgcgcg gccaaggact gggccaccat cggatatgtc
      421 atggccctgg ctggtgtggc ggtcgcgttg tggatccggc tcgcttcccg gcgggcccgg
      481 cacggtgaga ggacggacgg catcgaggtg aggaatcccc ccgctctcgg tcggcaggaa
      541 ggccggaccg gacgcgccgc gccgcgcgtg ccccgtccgc gttccgcgga gcccgaccgg
      601 cagaacaatg agccgctcgg cgcctcccgc gggtggggcc ggtgatcaca gcaaagccca
      661 ggctggcctg catcgatccg ccccggctgc tggccgggct ggacgaggcg taccggctgg
      721 accgcgtcgg ccatctgacg gtgcacgggc agatgcccgc cctgcgcgcc gatgagctgg
      781 tggccctcgc ggagaacatc gacctgcgcg gtcgtggcgg cgcgggtttc ccgttcgcca
      841 agaagctcac gtccgtcatc gagtcggcgg cgcgacggga cggccgttgc gccgtggtgg
      901 tcaacggcag cgagggcgag cccagctgtc tgaaggacac cgcactcctg ctgcacactc
      961 cgcacctggt catcgacggt gccatgctgg cggccgaggc gctcggcgcg gaggacgtgt
     1021 gcatcgcggt gcaccggccg gatgtcgagg agtcgctcgc caccgccatc gccgaacgcg
     1081 ccccgagcgg gccccggctg cgggtctccc gcaccccgga ccgcttcgtg gccggtgagg
     1141 gcggtgccgt catcaacggc atcagcggcg cgccggccat ccccaacggc cggaagatcc
     1201 ggaccagtga cagcgggctc gggggccttc ccaccctgct gtccaacacc gagaccttcg
     1261 cccagctcgc cgtggcggct cggatgggcg ccctgccgta ccgctcggtg gggctgccca
     1321 ccgagccggg caccaccctg ctgacggtgg cgggcaagtt cgtcatggag acccccaccg
     1381 gggttccgct gacctacatc ctggagctgt gcaacctcac ccccggccag ggcgttctgg
     1441 tcggggggta ccacggcaag tggctggacc cgaacagcat ccacgccacc accatctccc
     1501 gcgattccat gaagaagtac ggcgcccggc tgggcgccgg ggccgtgctg ccgatccccg
     1561 aggacacctg cccgctgggc gagaccgccc gggtggcccg ctggctggcc gcggagacgg
     1621 ccggtcagtg cgggccctgc ttcatcgggc tgcccgcact cgcggacgcg ctgggccagg
     1681 tggagcgcgg tggcggccag accgccatcg acagtgtgcg cgccttcatc aactcggtga
     1741 agaagcgcgg ggcctgtagc caccccgacg gtacggccgg ttttgtgacc accgcgctgg
     1801 acgccttccc ggaggagatc gagtcccatg cgctgggcat gggctgcggc cggccgaccc
     1861 tcggggtact gccgctgccc gaggactcca agccactggc gctgcccccg ggtccgtccg
     1921 cggccgatcc ccggcgcgaa cgggaaccgc gggggcgcat ggagcagctc atcgtggact
     1981 ggtcgctgtg ccagggtcac gggctctgcg cggacatcat ccccgatgtg ctcgaactgg
     2041 gcatggacgg ctaccccgcc tcggcgaaga tggatgtgcc gcgccagttc cgggcccagg
     2101 cggtccgtgc ggtccggcgc tgtccggcgc tggcgctgcg catcgaggag tagccgccgc
     2161 ccgcgccccc cggcgccacc cttcgacggc cgtcagagac ggccgtcgac cgctgtgcgc
     2221 tcaacgatcc ggcgttttgt gcgtccgatg acaaatttcg ggaccgctga acgtcgctcg
     2281 gcaccggctc cgtatgcaca tggcgtcggc gcaaacaccg ccggaccgag agagattgca
     2341 gaggaatggt catggagaag cggcgtcaga tcgcattcgc cggagtgtcc gtcgcgatgg
     2401 ttctgctgac agcggcgtgt ggcagcagca aggacaactc ctccggtcag agctccaacg
     2461 tccagcccgc cggcggtgcc cagaacgtgg gatcgggctc gacccccggc gcggctgccg
     2521 gctccggtgc ggccggcggc taccaggcgg gcggcgacac cggcgcggct gcgggctccg
     2581 gcgacaccgg ccagtccggc gcggccaagg aagtgaccgc caagaaggac cccaagctgg
     2641 gcgagatcgt gaccgacgcc aagggctgga ccctctaccg gttcgacgag gacaccccca
     2701 agcccgccaa gtccaactgc aacgacgcct gcgccaccaa gtggccgccg gtgcccgccg
     2761 acgacgccac cgccggtacc ggtatcgaca agtccaagct cggctcggtc acccgctccg
     2821 acggcaccaa gcagctgaca ctcgagggct ggccggtcta ccgttacgcg ggtgacacca
     2881 aggcgggcga caccaagggc catggcgtgg gcggcacctg gaacgcgctg gcgcccaacg
     2941 gcaagccggc cggcaagaag gccaccggtg ccgacgagag cctccagctg atgctgaaca
     3001 acaacgccga actcggcaag atcatcgtgg atggcaaggg catgacggtc taccggttca
     3061 acaaggacag cgcctggccg atgaagaccg gctgcctcgg cgcctgcctg gacacctgga
     3121 agcccgtcaa ggccgtgaac acctccaagg tcgccggtct ggacgcctcg aaggtcacct
     3181 ccttcacccg tcccgacggc aagaagcagg ccgcgttcga ctgctggctg ctctacacct
     3241 tcaccggtga caccaagccc ggcgacacca acggccaggg caaggcgggc cagtggttcg
     3301 cggtcaccga caagggcaag aaggcgggcg tcgccgcggc gcagtagaca gaaacacggt
     3361 caccacagcg gggggcccga ggccgcagag cacccgggcc tcccccgccg gaccctcaac
     3421 gggaggttgt agtgtcaccc gcactccgtg ccaccgcgct ctgcgcggct ttgctgttcc
     3481 tgagcggctg cgccgggaac agcacggcgc acgacgccac gaagcatccg cgggagaagg
     3541 cggctccttc cgccaaggag ttcggcaaag ccggcaagcc cgccccgctg gccgggatcg
     3601 ccgacgccat cggctgcaag gcgaaggtca tcaccgaggt ggatgagctg cggcagggtt
     3661 cctgcacctc cggcaagaac cggtacacga tgctcacctt cgccaccgag cagggccagc
     3721 acgactggct gtcggtgtcc aaggactacg ggggcaccta cctggtcggc aagcgctggt
     3781 cggtgaccgg tctgtccacc ggctcgctct cgtccctgcg ggagaagatc ggcggggaca
     3841 tcgagcacgg gatgtccatg tcccacggcg gctccggcgg ctcgcacgaa ggctccggtt
     3901 cgaagggcgg ctccggctcg cacgacggct cgacatcgca cgacgggatg gacggcatgt
     3961 gaaccgcgcc gccgaccagg tggcgaagcg tttcagctgg tcatgcgacc ctggtttcgg
     4021 cccacctgac cagatcgggg tcggcgaggg tgtccaccca caggtccacc ggggggcgtt
     4081 cgtccgccgg tttccgcggg ccgacaccga ggactctcag cccggcgcgt gaggcggaga
     4141 gaaacccgca gtgggagtcc tccaccgcca gggcgtcgcc gggggccgca ccgcacagcc
     4201 gggcggcctc cagatagacg tccggatacg gtttgggccg catctcgccc tcggggacca
     4261 gcacatggcc gaagtggcgc agcagtcccg ccttggcgag cccgtcctcg accacgtcac
     4321 gcgggcagtt gctcgcgatg gccagcggcg cgaacgccgc ggccttctcc accagttccg
     4381 gcgccccggg catggtgacc gggtgctcgg ccaccagggt gcggaaggcg tcgagcagtt
     4441 gcccggtcat ctgcccggcg agttcggggt gcccggcgaa ttcggccagc atacggccgc
     4501 attcgctgta gtggagtccc ttggtgcgcc gggcgaactc ttcggtgggg gcggtgccat
     4561 gctctctgag caccatgtca cgcgcctcct cccagtgccg ttcggaatcc atgagggtgc
     4621 cgtcgcagtc gaagacgatg gcggctgggg tccatgcgag gagcttttcg gctgtcatct
     4681 gcctgccgtt ccatgggagg tggaggaatc gacgcagggg cgcctgtacc tgcggggagc
     4741 agcgcagtgg atttcctggc ggctcggtgc gcgaatgcgt tcaccgctgc tgcccgatgc
     4801 gcgaacgcct tttcgacgta cgcgacatgg ggggccgtac agacggagaa tccgcctgcc
     4861 cgaaacataa cgatcactac gttaattgga tgacccggag taagcaaggg atcaccgaac
     4921 gtcggcgatt tgttcgcggt cccgcgcgtt cgccgctcaa cgcgttcgcc gtacggactg
     4981 tttgacggac agaagtacgg tcagaggtac tggagcaccc cgaccgcggt gcacatctcc
     5041 gcctggcgca gccggaagac acaggtcctg ccgtgggagg tgcactcgac cacggcgggc
     5101 ggatacgact cggtgaagcg gatcgccttc agccggcagg cgacgggccg ggtcagcgtc
     5161 ccgtcggtgg ccagcgcggc ggcctgccgg gcgctctcca gcagcaccag gcccgggaca
     5221 tggtcgctcc agtggtcgta gaagaacggg tggcgggggt cggcgggatc caggtacacc
     5281 actcccccgt cccgtaccac cagcacgtcc cgggcggcgg gaacggccag ttgacccggg
     5341 gacggccgga ccgcggcgcg cagtctgcgc cgtgcggtct gtcccgaggc ggccgagcgg
     5401 gtctccgcgt aggaggtgcc gtcgaggaag cgcgcggtgc ccccggccca ggcgaaccgt
     5461 ctgccgctcg ccgagagggc gatgtcgagc cgcatcccgg acacaaagcg cccggagggt
     5521 gtggtgcgga ggtccgtcac ggcggcgtgg caggtgatgt cggtggcgcc gtgcggcgcg
     5581 cgcggctcgg cctcggggtc gagccggtag aacaggtcgc ggatcaggaa gtgtgcgccc
     5641 gcgggcaccg cgtagtaccg ggtgggaagg tagatcccca gttcacgcag ggtttccgcg
     5701 atcatcagcg gatggtgtct gccgtccccg ccgtgcggga aggtcggatg cgaacggggc
     5761 cactgggcgg cggcctcgaa gcggtcgtca cccagcgtac ggacgtcggt gagcagcacc
     5821 tcggccaccg agacccggtg cacggcctcg cgttccaccg tgcgggacca gctgagcggc
     5881 cgcccggtgg gaccggacgg ttccgaaagc ccgcacgcct cacgaacgat caccttaaaa
     5941 tactaataat gtttgacctg tctcgcctca tctccatgac ggggcggaat tccgctaagt
     6001 tcgctccgac acacaaaact gctgatgaag gacggcgaac acgtgcaaga gcgggccgag
     6061 caaacgcgcc gatcccttct ggaggcggcc gcatcccttt tcgacgaacg gggatacgcc
     6121 ggaacgagca tcagtgacat cacccaccgg tccgggcaca ccagcggcgc catgtatttc
     6181 cactacacga gcaaggaaag actcgcgctc gccgtggtgg aggagcactt cgccacctgg
     6241 ccggcgctga tcgagcagca caccgccatc gacgcccccg ccctggagca actcgtgcgg
     6301 ctcagcttcg cggtggcccg tgccttccgc gatgacatcg tggtgcgggc gggggcccgg
     6361 ctgtggacgg agcgcaccct gatcgaggca ccgctgccgc cgcccttcgt gggctggatc
     6421 gacgcggtgg gaaagatgct ggagcaggct caggccgacg gcgagctcgc cccgcatgtc
     6481 gatccgcagg ccgcggcccg gaccgtggtg tgcgccttct tcggtctgca caccgtctcg
     6541 gaggcgctcg acggacgacg gctggtcgag gaccatctca ccgatctgtg gacgctgctg
     6601 ctcccctcgc tccaggcccg ccccggcgcc accgccgaac tgctggcggc ggcccggggc
     6661 acggacccgg taccgctgcg cgccgacgac gaggccgtcg gcacggacga gatgtgaagc
     6721 ggcggcctac ttccagatct cgccgaccca ctcgggatgg tcgacgaacg ggttgcggtt
     6781 gtgctggatg gtgtcgtaaa tgacatcgtt gcggtgcttc tcgaaggtgt ccggcgggtc
     6841 ctcgtcgttc cacttcttca gcacggacag ccgcccgagg ttgggcctac tgccgttgcc
     6901 cgccgtgtcg ttgacctcca ggtcggggaa gtcgtcgtca ccctcgtaac gcaccgacat
     6961 gtagagcacc atccgggcca catcgccctt gacctggtcc cgcggctcga aggagtcgtc
     7021 gtcggtacgg ttgccgggcg ctccctcgac ctcggacccg cccgcgtcga agtccttgtt
     7081 cccccggatg ctgttgaccg tcacattggc cggacgcaga tggtggacat cggtcccggg
     7141 gccggtcgcg gtgccgaagt cgccgtgcga cttggcccag acgtgctcac ggttccactg
     7201 gtcgggtccg gaaccgttcg cgtttttgct ctgtgaagtc ccggtgtaga gcaggatcac
     7261 atgggcggag ttgtccgggt cctcgtcggt cttcttgagg gcgtcccaga cctcgtcata
     7321 cgacagcctg gtgacgtcgg tgctgatgat cgtgtgcagc gcgtccttca aggggtcgcc
     7381 ggtcttgccg atcgcgtcct tgtagtaggt gtcgtcgtac ggcgtggatg tggccgtgac
     7441 ctcggcggtc gccggggccg gtgcggtgac ggcgagggtg gcgacgccgg ccagcagggc
     7501 cagggatttc cagcggttga cacgggtgac gtgcacaggt ggtgtccttt ccccgattta
     7561 cgaccgttgt tcgatccccg aaagcgtgtc agacacatga tcgtacgatg gtgaacaacc
     7621 cgcagaggtt ctgtgtcagg gccatgacca atcgggagtg gtcaacggcg cccttcgcgc
     7681 caccgatcag gaccggccgt gccaggacgc ccagagcgcg gcgcaggccc ctgccgacgc
     7741 accagctcct cgtgcgggcc cagttcggtg atccgccccc cgtgactggt gtcctcgacc
     7801 agtgcgcccg agggccgcgg tcgtggctgt cggcgaggag ttgggcgtgc ggcccctggg
     7861 cggcggcctc gccgccgatg ccgcggagcc cccgcagtcc gctgacgaag tcgaccgctc
     7921 ggccggtcgg ttcggcctgc tgttcgcgca gccgctgtct cggcgagcgc ggcgccgagc
     7981 tgggagaggt cggccacgcc cgcgtgatgc gctgggccgc gcggtcggag gcgccgagcc
     8041 gggtggtgag ggcggcgcgg tcgcgcagga tgcccgcggc ggcctggatc gctccgtcgg
     8101 gcaccagcgt ctgggacagc acacggacaa tgccgtacag gacacccata acaagagaat
     8161 ttcgctgatc agcggcacgt cataacagat aggatgaggg ggagcgccgg tccggacgtc
     8221 cgggatccgg atcggggaag cgtcgcatgg cgaaggcgat gccaacgacg cccggggcct
     8281 tcgcgcggtg attttttccc gcgtacggcc tggggccggg cgtggaccgg ggagggctgg
     8341 gcactcgccc cacagatgac gtagccactg tcgtacgaac cttggaggac accgtggcac
     8401 ggcagaccga cccgttttct ccgcccccgg cactgcgtga ccccgggcca ccgtcgccca
     8461 ctccgtcacc cggcccgggc ggtccggagc cgggtcccgc accgggaccg gtgcccggcc
     8521 cgccgccggg accgccgccc gacccggtgc ccactccccc gcccagcccg ccgccgagcc
     8581 ccacaccggg gccaccgccg ggcccgtccc cggatccggt gcccaaaccg ccgggccccg
     8641 acccggtccc ggatccgtca ccggccccgg ggccgctcac ccagaccctg tccggtggat
     8701 cttgtcgagc ccgcggcgtc tggcaccgcg cccccagcca ccgcggggag gcgccccctg
     8761 caccagacgc cgcgggctga tcctcaaaga tccaccggac aggacccata cccgcccgcc
     8821 cccgcgccgc gccccgccgt gccctgatcg caccgcgggg cgcggcgctt cgctccggcg
     8881 cgcgattgcg cgagaaccat cgttctccta cggtggtggc cagcgtcaga acgatcgtac
     8941 tccgagtggc gtaaccgcga tgtgttcctg cgcggccgga gtgagatcgt cgaattcctg
     9001 accgcgaagt ggcggcgcga actcggctac cggctgcgca aggagctgtg ggcctgggcc
     9061 ggcaaccgga tagccgtccg cttcgagtac gagcaccacg acgccgcggg ccggtggttc
     9121 cgcgcctacg gcaacgagaa ctgggagttc gacgagaacg ggctgatgcg caagcgctac
     9181 gccagcatca acgacctgcc catcaccgcc gaggagcgcc gcatcgcggt gtgacggaag
     9241 cggggcgggc ggcggcctgc gaccggatgg cgaggagagg caatggagaa gcagatgtcc
     9301 cacggcaccg ccccggcccg gcccgtactg gagcccgccg ccgccgagct cgccgaagcc
     9361 agcgccaagc cgccgttcct gtaccagatg tcgcccgccg agggccgcag ggcggttgac
     9421 gaggcgcagt ccggtccggt acccaaaccg gaggtggacg aggagtggat cagcgtcacg
     9481 ggcatcccgg agggcggcgt caaggcccgg atcctacgtc cgccgggggt gggcggcacc
     9541 ctgccggtga tcctctacat ccacggcgcg ggctgggtgt tcggcaacgc ccacacccat
     9601 gaccggctgg tccgggagct ggccgtgcgg gtcggcgccg cggtggtctt ccccgagtac
     9661 gacctctccc ccgaggtgca ctacccggtg gcactggagc agaactacgc ggtcggacag
     9721 tggatcgtgg cccatggcga gggggcgggc ctcgaccccg cacggatcgc ggtgaccggc
     9781 gactcggtgg gcggcaacat gtccgccgcc ctcaccctgc tggccaagga gcgcggtgat
     9841 ctctccctgg tccagcaggt actgctctat cccgtcaccg acgcggcctt cgacaccggc
     9901 tcctacgagc agttcgccga cggctactac ctcacccgcg aggggatgcg gtggttctgg
     9961 gaccagtaca cgaccgaccc ggcccagcgc gccgagatca ccgcctcccc gctgcgcgcc
    10021 gaccccgccc agctcgccgg gctgcccccg gccctggtga tcaccggcga ggccgacgtg
    10081 ctgcgggacg agggagaggc gtacgcggcc aggctgcgcg cggccggggt gccggtcacc
    10141 gccgtacgga tgcagggcat catccacgac ttcctcatgc tcgacgcgct ccgtgacacc
    10201 aacgccgcgc gggccgcgct cgcactggcc gtggacaccc tgcgcaacgc gctgcacccc
    10261 gcgtgagccg gcgcggggag ctcacttccc ggcggagcgc ttcgcctgcc gcttggcctc
    10321 ctgcttgtgg gcgcggacct tgtccagcga ctcgggaccg gtgatgtcgg ccaccgagcg
    10381 gtaggcgccc tcctcgccgt agccaccggc ggcctcgcgc catccctcgg gtccggcccc
    10441 cagccgcttg ccgacgagcg ccaggaagat ctgggccttc tgcttgccgt acccgggcag
    10501 tgcgttcagc cgcttgagca gctcggcgcc gctgtccgca tcgcgccaca ccgcctccgc
    10561 gtcaccgtcg tagtgctcga cgaggtactg gcacagctgc tggatccgct tggccatcgc
    10621 cccggggtag cggtgcacgg cgggcttctg cgacagcagc tccacaaacc gctcggggtc
    10681 ctgggcggcg atctcctggg cgtccagctc gtccgacccc atgcgctgcg cgatggtgta
    10741 cggaccggcg aacgcccact ccatggggac ctgctgatcg agcagcatcc cgatcatgag
    10801 ggccagcggg ctgcgcccga gcaaggcgtc ggcctcgggc tgctgggcga gtcgaagggt
    10861 ggcgtgcatc cccggtgtgt acccccgggg ccacggcccc gaaccccgca ccctccgcat
    10921 tctcccaaaa gcg
//
