LOCUS       NZ_WQMW01000051        10267 bp    DNA     linear   CON 07-APR-2024
DEFINITION  Bacillus paranthracis strain ELWA-3_3298 Contig51, whole genome
            shotgun sequence.
ACCESSION   NZ_WQMW01000051
VERSION     NZ_WQMW01000051.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus paranthracis
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Matson,M.J., Anzick,S.L., Feldmann,F., Martens,C.A., Drake,S.K.,
            Feldmann,H., Massaquoi,M., Chertow,D.S. and Munster,V.J.
  TITLE     Bacillus paranthracis Isolate from Blood of Fatal Ebola Virus
            Disease Case
  JOURNAL   Pathogens 9 (6), 475 (2020)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  AUTHORS   Matson,M.J., Feldmann,F., Anzick,S.L., Martens,C., Massaquoi,M.,
            Sprecher,A., Giuliani,R., Edwards,J.K., Drake,S., Feldmann,H.,
            Chertow,D.S. and Munster,V.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2019) Research Technologies Branch/RML Genomics
            Unit, National Institute of Allergy and Infectious Diseases, 903
            South 4th Street, Hamilton, MT 59840, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-AUG-2019
            Assembly Method                   :: MIRA v. 4.0.2; Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 32.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013357905.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 07:43:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,895
            CDSs (total)                      :: 5,741
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 154
            rRNAs                             :: 13, 15, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 13, 12 (16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 85 of 248
            Pseudo Genes (incomplete)         :: 165 of 248
            Pseudo Genes (internal stop)      :: 39 of 248
            Pseudo Genes (multiple problems)  :: 34 of 248
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:58:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 753
            Orig. end                         :: 11020
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WQMW01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5267](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5267)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(697..1140)
                     /locus_tag="GI596_RS28290"
                     /old_locus_tag="GI596_27990"
     CDS             complement(697..1140)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="GI596_RS28290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_27990"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757633.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGKNGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHITVKGICYISEAFFEERGHHAIFFNFLGEIIGGETNITRPKEIE
                     EITWMELHRAEPHLRIPEHLLNILQRKETVPYFFNGTIIHQSS"
     gene            complement(1350..1775)
                     /locus_tag="GI596_RS28295"
                     /old_locus_tag="GI596_27995"
     CDS             complement(1350..1775)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="GI596_RS28295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_27995"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_175251971.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKYISDEEKKPSEKISFIVKNE
                     DEEIVGGITAITFWHHVHVDFLWVSEEYRHEGYGTKLIKLIEEFALEKECSLINLDTFS
                     FQVPAFYKKHGYKVIGVSEDHPKGHNRYYLEKRLENI"
     gene            complement(2215..3423)
                     /locus_tag="GI596_RS28300"
                     /old_locus_tag="GI596_28000"
     CDS             complement(2215..3423)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="GI596_RS28300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28000"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000815152.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYNPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIVLGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFEPTFIIIGVLSVISFVISILIRPPKAEKKKETQHLYR
                     KVA"
     gene            complement(3708..3851)
                     /locus_tag="GI596_RS28305"
     CDS             complement(3708..3851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="GI596_RS28305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            complement(3883..4899)
                     /locus_tag="GI596_RS28310"
                     /old_locus_tag="GI596_28005"
     CDS             complement(3883..4899)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="GI596_RS28310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28005"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_001992204.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEQNHSSAAALCIMKDNKIVLEHYSGYHSNT
                     STSKKVTASSQFNVASTRKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPVLLSKTTI
                     RHLVTHSHGLEETNDGIIFREFEPGQGWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPANFQETGWRIQQDDNLVAVVDDPNKDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGMIHDKQIVPKEVIKIATSLQSPTYANKELPQNGLFWFVQNEPAQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVVDTFRNSNRA"
     gene            complement(5001..5267)
                     /locus_tag="GI596_RS28315"
                     /old_locus_tag="GI596_28010"
     CDS             complement(5001..5267)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="GI596_RS28315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28010"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001992149.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.9e-50, bitscore:
                     157.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCIGCVGCFGCFNCWNWWII"
     gene            complement(5471..5917)
                     /locus_tag="GI596_RS28320"
                     /old_locus_tag="GI596_28015"
     CDS             complement(5471..5917)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="GI596_RS28320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28015"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000790757.1"
                     /transl_table=11
                     /translation="MKNKFHHIVRAVMIKDKKLLVAEYIGHHYFLPGGHVEVGESAENA
                     LIRELREELGVNCSIKQFLGVIENRWQDREVLHHEINHIFEIDSQELHIDFIPKSKESH
                     LAFHWIDYNQEALHTYKIMPEPSVKELLERKLSDELLNCWISNF"
     gene            complement(5943..6281)
                     /gene="cutA"
                     /locus_tag="GI596_RS28325"
                     /old_locus_tag="GI596_28020"
     CDS             complement(5943..6281)
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160602.1"
                     /locus_tag="GI596_RS28325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28020"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160783.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIEMLRNELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLKGASPFNGEIGQICEGQECKIEIKCKRDLVKDALEVINEIHPYETPMIYIIPI
                     LNDYFNQI"
     gene            complement(6312..6770)
                     /locus_tag="GI596_RS28330"
                     /old_locus_tag="GI596_28025"
     CDS             complement(6312..6770)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="GI596_RS28330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28025"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001025603.1"
                     /transl_table=11
                     /translation="MNIQLKVVTRENWEDALKLQVKDNQTKFVPSVAVSLAKVYIKPDG
                     DAVEYQPFAIYNGDLMVGFVMHAVVKETTDMYWINGFIIDQKQQGNGYGKAALQESIYL
                     IKNTFKACKEIRLTVHKDNISAKKLYESYGFQPLGHDYDGEEVYRLFV"
     gene            complement(6877..>7668)
                     /locus_tag="GI596_RS28335"
                     /old_locus_tag="GI596_28030"
                     /pseudo=""
     CDS             complement(6877..>7668)
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107015.1"
                     /locus_tag="GI596_RS28335"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28030"
                     /product="DMT family transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="TLLRLLIASFILLLFSFLYKLRLPNLKDIPAIFLFGALGFTFYHI
                     ALNYGEKTVNAGSASLIVSVTPIVTAVLAFVFLNEKMKANGWIGGVISFIGIAFISFSQ
                     GGSIQFNSGGLFILLAAVSESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGMY
                     QEILVAPLEVNLSVIYLGLFPTVLPYIALAYIISHTGASEATSSLYLTPVIACFIAWVW
                     LGEVPTFISVVGGVITILGVLIAHVSLKKDIRSEKYNDITG"
     gene            7886..8347
                     /locus_tag="GI596_RS28340"
                     /old_locus_tag="GI596_28035"
     CDS             7886..8347
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001982483.1"
                     /locus_tag="GI596_RS28340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28035"
                     /product="DUF2306 domain-containing protein"
                     /protein_id="WP_000021737.1"
                     /transl_table=11
                     /translation="MSIFNILLTIHILFGTICLITGIVAMVAQKKKGKHTEWGEIYHAS
                     YVVITLTAIILSIINWDKIAYLFYIAIFSYAFAIYGYLARKKRWKNWLHHHIKGMLGSY
                     IGAVTALLVNVGIHIPIINLLPPIWFWFLPTLIGIPLVASVSKKYKKRS"
     misc_feature    complement(7896..7913)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.32"
                     /tool="antismash"
     gene            complement(8499..8846)
                     /locus_tag="GI596_RS28345"
                     /old_locus_tag="GI596_28040"
     CDS             complement(8499..8846)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022865.1"
                     /locus_tag="GI596_RS28345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28040"
                     /product="hypothetical protein"
                     /protein_id="WP_001101470.1"
                     /transl_table=11
                     /translation="MNVLAVVPRLYVSDLQTHLEFYKILLRQEKPQEFQMNGTHVIRFT
                     NLLLIEEVDEPLKKRVAVTIVIDNLQEVKKLILNYNGVIITEPIQIPTGMKMIVRHPDN
                     NIIKYIEPTLK"
     gene            complement(8943..9806)
                     /locus_tag="GI596_RS28350"
                     /old_locus_tag="GI596_28045"
     CDS             complement(8943..9806)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725538.1"
                     /locus_tag="GI596_RS28350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GI596_28045"
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="WP_000725532.1"
                     /transl_table=11
                     /translation="MKKIYIVTIICAFLVIGTFVYYKVNELKYTKAINQSNHIKNISHR
                     GASAYAPEHTIPAYTLGQQLKADYIEIDLQMTKDGHLVAMHDETLNRTTNGTGLVKEHT
                     LEELKQLKADSSFNEKHPNLAKREYENAKVPTLEEVIEVFGHEANYYIETKSPDEYPGM
                     EEKLLEIINHYEIQDKVIIQSFSEESLQKLHSLYVNIPLVQLLSYKKAVQLTESEIKKY
                     KTYCIGLGMNYKYIDAAYVKRIKKHGLEVHPFTVDNEQDMKRLLLWGVDGIFTNYPDRL
                     HSLLKS"
ORIGIN
        1 ataaatagaa acgaattcta atttgctgtt cattaatttt aaaagctcat taatggttgc
       61 acttggtaca atacaattcg ctttcatatt agtagaaatt aatgtttcac gtatagctag
      121 tctatgagag tcagttgcag cgcatattaa tttgttatgg tctaactcga tatgtactcc
      181 agtaagaacg ggtctagatt catttttcgc tactgcaaag actgtttgtt taaacgcatc
      241 aatcaattgt tttgtttcta tttgtatttc tgcatgatcg tctatttgtg gtacgttagg
      301 aaattcattc gctggaaagc catttaagtt taatgtgatt tcttctgatt gtatggtaat
      361 tgtttgctca ttcttacttt ttattactat atcgcttggc attttcttta taatttcaat
      421 gaagtatttt gcaggtacaa caattgttcc tgcttttaag gtagtcgtaa tttgttcgtc
      481 atccatggaa ctagggatga atttttcgat gaaaatattt gaattacttg cgattaaagt
      541 aattccagat tgatctgcag ttatttttat accggatagt ataggaatta tagctttggt
      601 agatattgct ttacttactt ctgaaagtgc ttgagtgaaa tgtttatgat taacgataaa
      661 ctccattttt tccccgcctt acatgaaatg atagttttat gaagattgat gaatgattgt
      721 tccgttaaaa aagtaaggta ctgtttcttt cctttgtaat atatttaata agtgttctgg
      781 tatacgtaaa tgcggttctg ctctatgtaa ttccatccaa gtaatctctt caatttcttt
      841 tggccttgtt atatttgttt ccccgccgat tatttcacct aaaaaattaa agaatattgc
      901 atggtgtcct ctctcctcga aaaaagcttc gctaatgtaa caaataccct ttacagttat
      961 atgtagacca gtttcttctt tcacttcccg aattactgcc tcttctaacg tttctcccaa
     1021 tttaaccgcg ccacctggta gtgtgtaata agaaccattc ttccctttgt tttttaccat
     1081 taagatcttt tcatttgttt tatcgtataa aagtgcatat gtaacattta ctttcttcat
     1141 ctaagtccac ctctaataat gggaattttc tatctttgtt acattctacc atttaatcgt
     1201 tttaaaatat aaaaaattaa tagagtattt catttatcta tccttagtaa aggtaataat
     1261 tgatccccct tcatttctaa ataaaaagaa cgtattttga aagtaattat tcaacgtacg
     1321 ctctttatgg tttgattgat tttgggattc tatatatttt ctaacctttt ttccaaatag
     1381 tatcgattat gccctttagg gtgatcttca cttactccaa ttactttata tccatgtttt
     1441 ttataaaaag caggtacctg aaagctaaaa gtatctaaat tgattaggct acactctttt
     1501 tcaagtgcaa attcttcaat aagttttatt aacttggttc cataaccttc gtgtctatat
     1561 tcttcagaaa cccataagaa atcaacatgt acgtggtgcc aaaatgtaat tgcagttatt
     1621 ccacctacta tttcttcatc ttcatttttc actataaaac taattttttc tgaaggcttt
     1681 ttctcttcat ctgaaatata tttttgatta tattcaatta ctttttctct tatgtaatca
     1741 ctatcttctt ggacccattg ttttactatt ttcattttaa ttatcacgct ccttttaaag
     1801 tatctattca atttaataat cgtaatatcc tgtctaactt aatagtcgta gtgcactgtt
     1861 tcatttcact aaaattgtaa aaagccgtac tacattttat ttgtagtaca gctttttacc
     1921 gcgtttgatt ctttttaatt aaaacttccg ttagatggta tataaatgaa atgtttgcct
     1981 aataacaaag cgcattaact ttagttttat aatagcattc atattctttt ataaaacaat
     2041 atgaatattt tacttatctg ctacttgatt cgttaataaa taaaccactt tattgcttct
     2101 aaaatcattt cattttaaat taagtaagtg tattcttcct acacaatggc taaaaaggga
     2161 aaatcaaatg ttgtgtaata taaaaagagc cctttatgag gactcttttt aaacttaagc
     2221 tacttttcga tataaatgtt gtgtttcttt tttcttttct gcttttggtg gacgaattaa
     2281 aatagatata acgaaagaga taacagaaag tacgccgatt ataataaagg ttggttcaaa
     2341 tcctccaaga agcgccccga tgaatgatcc tgcaagtgca ccaaatccga aaccttggta
     2401 tacaatccca taatttgtac tatgattttt caatccgaag aaatctccaa caatagctgg
     2461 gaatatagtg atattaccgc caaagcaaaa agcaacactt gctacacatg cgaaatatat
     2521 gccgtaattt agcggaatga aacttaatgt aaagactgac aatccaatga taataaacgt
     2581 tgcagaaacg attttcattc gacctatttt atctgataac gttccaagaa caatacgacc
     2641 tactgtatta aaaattgcaa tcattgcaac ggcgttagcg gcagttgctg cgctaagtcc
     2701 aacgagctgc actccgatat cttttaccat accgattaaa tatagaccgc ccatacacga
     2761 tgtgaatagc ataaaaaaca aaagatatac ttgtttcgtt cgcatcattt cacgcggatt
     2821 atagtcattg tgtaatgttt ccgttacagc attacttaca attgcctcac gtaagaagaa
     2881 cgatccaatt aatactaaaa gcaaaacgat aatgccccaa tataaaaagg cttgtgatac
     2941 accaaggctg tcgatcagat ttccgtttat atatctaaag attaagctgc ccattccata
     3001 tgctgataca gatatacctg aaataagccc tttccgattc ggaaaccatt taattaaatt
     3061 agatagtgat gtgatataag cagttccatc ggcataacca actactacac cagctaataa
     3121 atatagtaat ggtaaggagg aaacttgtga acttagtatt aaaccgagtc ccagcacaat
     3181 ccctgcagta gcgatgagtt ttcgaagccc taatttttgc tgtaactttc ctgcaaataa
     3241 tgttgaaaat gataaagaga agcttgtaat ggagaaagtt atagcaactg aattaaggtt
     3301 ccatccaaac ttacttacaa ggggctgatt aaataaactc catgtataaa ttgttccaag
     3361 gccaatttga acaatgattg tacctagaac aattagtaac ggatttatag aagtttgttt
     3421 catactaact gtccccttct tttatcgtga catgaatttt cacagttagt ataaccatca
     3481 taaaaagcaa gatactgcat gaaaaatgga aattgaattc atgtacacaa aaagacactc
     3541 cgttatgaag tgtctttttg caggttatat taggtttact taaagaaagc tttgttaaga
     3601 taagacattt tcaaatgctt atgtatatag agtaccaaaa gttttcaatc atgtgatggg
     3661 acttaagatg tatttttgat tcatcatatg gtcggataaa cgaaaaatta atattcaatt
     3721 tcatcaaaat ctttcggctg cggtaccgtt tgcatattag ctgcatcttt tggatcgcta
     3781 tagatcgcat tttgctctgt atttacatct tttatatttt gctttttatt tttgttcact
     3841 ttatctttca ctttcaatca ctcctttttg ttataggagt aattatgccc tattactgtt
     3901 acgaaatgta tcaacgacta aattactaaa ttcacgtaaa taatataaat aattatcacc
     3961 gccgtagttg tatcttttat tatacatttt cgcaacaact acattatatt caggtattac
     4021 taatatagtt ggtcccgtaa ttcctaatat ttgatacgag ccttttggta cacgttcgcc
     4081 tagttcactt aattgtgcag gttcattttg aacgaaccaa aacaagccat tttgtggtag
     4141 ctctttattt gcatatgttg gactctgcaa actcgtagcg atttttataa cttcttttgg
     4201 aacaatttgt ttatcatgta tcataccttg atttaaatga aggttgcccc actgtgcaaa
     4261 ttctctagcg gagacaaaca gatttttttc agtaccgtca tctactgtac caatttcact
     4321 aattgcgtct ttatttggat catcaacaac tgcaactaaa ttatcatctt gctgtattct
     4381 ccatcctgtt tcttggaaat tagcaggttt aaacacacgc tcttttaaca attcaggaaa
     4441 acttttgtta tatagctgat aaataagacg tgtcatcatt cttacattaa tatctctata
     4501 cgcccatcct tgccccggtt caaattcacg aaaaattatc ccgtcattcg tttcttctaa
     4561 tccgtgcgaa tgtgttacta aatgtcttat cgtcgtttta ctaagcaata caggatcaaa
     4621 gtctttaaaa tattttatcg cttcatcatc aatagagttt attttcccct cgtaaagcgc
     4681 atacgctatc attaatccta aataactttt tctagtagaa gcaacgttaa attgtgaaga
     4741 tgctgttact ttcttgcttg tagatgtatt tgagtgatag ccactataat gctccagtac
     4801 aattttgtta tctttcatta tgcaaagcgc tgctgcagag ctatggtttt gttcttttat
     4861 attttctacc caagaaatca acttatcata tgtatacaaa taaatccccc ttaatacaca
     4921 taaaaattac agcttgaaag aaaagtgcaa aatagttttc acacctttcc ttcaagcaaa
     4981 atatctttga tgttctatac ttaaatgatc caccagttcc agcaattaaa gcaaccgaaa
     5041 catccaacgc atcctatgca tccgccgcaa cgacctgcac aaccaccaca tctgccgcca
     5101 catccgccac agcgtccacc acaacctccg catccgccgc aacgacgtgc atcgtcttga
     5161 atgtagtagt atggatattg actttgttgt tgatcccaat acacaacatt accagtttga
     5221 taatcattaa ggtttaatga ttgtagttct tgttgaaact gattcattat gaaacccctc
     5281 catttataaa tataaattcc gtccacatat tgaagttaat aaattttaat aaacaaagta
     5341 gaaacaaatg gctatatact tgaatacgta catcaacaaa atatgaactt acactaggtg
     5401 atgtaccttg tttttcagcc tattttctta atcacttttt gctggacgaa tttattatta
     5461 atagaggttt ttaaaaatta ctaatccaac agtttagtag ttcatcactt aattttcttt
     5521 ctagtaactc tttaactgaa ggttctggca tgattttata agtatgtaaa gcttcttgat
     5581 tataatctat ccagtgaaac gccaaatgag attctttaga ttttggtatg aaatcaatat
     5641 gtagctcttg tgaatctatc tcaaaaatat gattgatttc atggtgaaga acttctcgat
     5701 cttgccatcg gttttctatg actcctaaaa attgttttat actacaattt actccgagct
     5761 cttcccttag ttctcttatt aatgcattct cagctgattc gccaacttca acatgaccac
     5821 ctggtaaaaa atagtgatgg ccaatatatt cagccactaa caatttttta tccttaatca
     5881 taacagctcg tacaatatga tgaaatttat ttttcaattg ttattcctcc cattcattta
     5941 ttttatattt gattaaaata gtcatttaat atcgggataa tataaatcat aggtgtttca
     6001 tacggatgaa tttcgttaat aacttcaagt gcatccttta caagatcccg tttgcacttt
     6061 atttcaattt tacattcttg cccttcacaa atctgaccaa tttccccgtt aaaaggtgat
     6121 gcgcctttta acggtctcca gtatcctttc acagaactat aggacagaca atgatcatat
     6181 tctcccgttt tacaagcacc aattttattt aactcattcc tcaacatttc aatgtattct
     6241 tcagggataa atacttcaat tttgacttca gtaaattgca taattcattt cacacctttg
     6301 ctacataata attaaacgaa taaacgataa acttcctcac catcataatc atgtcccaat
     6361 ggttgaaaac cgtaactttc atataatttc tttgcagaga tattatcttt atgtactgtt
     6421 aatctaattt ctttacacgc cttaaacgta ttttttatta aatatatact ttcttgtaat
     6481 gccgcttttc cataaccgtt accttgctgc ttttgatcga taataaatcc gtttatccaa
     6541 tacatatcag ttgtttcttt tacaacagca tgcataacaa aaccaaccat tagatcgcca
     6601 ttgtatatcg caaatggttg gtattctaca gcgtccccat ctggttttat atatacttta
     6661 gcaagtgaaa ctgctacaga tggaacgaat ttcgtctgat tatctttaac ttgcagcttt
     6721 aaagcatctt cccaattctc tctcgtaaca acctttaact ggatattcaa tattttaaac
     6781 ccctttatta agaatatatt tacatattca acgtagtgaa ataattccct gtttcttaat
     6841 tataaaaaac agggaattat catttctaaa tagaatttat ccagttatat cgttgtactt
     6901 ttcacttcga atatctttct ttaatgatac atgcgctatt aaaactccaa gtatagtaat
     6961 tactccacca actaccgaaa taaaagtagg tacttcacct aaccataccc aagctataaa
     7021 acaagcaatt actggcgtta aatatagaga gcttgttgcc tcggaagcac cagtatgaga
     7081 tataatatag gccaatgcaa tatacgggag tactgttgga aataaaccta aatatataac
     7141 gctcaaattc acttcaagag gagccaccag tatttcttga tacattcccg gtaagaaaat
     7201 aagcatacat acagtactag ataaaattgt atatatggta aatggcaaga agccgtactt
     7261 ttttaagtac gatgtttgaa aaacgaaaaa tagactttct gaaacggctg ctaataatat
     7321 aaataatccc ccgctattaa attgaataga ccccccttga ctaaatgata tgaaagcaat
     7381 tcctataaag ctaattacac caccgatcca tccatttgct ttcatttttt catttaaaaa
     7441 aacaaaagca agaactgccg ttactatagg agtaactgaa acgattaaac ttgccgatcc
     7501 agcatttact gttttttcac catagtttaa tgcaatgtgg taaaaagtaa atcctaaagc
     7561 accaaataaa aaaatcgctg gaatatcttt tagattaggt aatcgtagtt tgtataaaaa
     7621 cgaaaacaaa agaagtatga atgaagcgat taacaaacga agtaaagtag cttgtcattc
     7681 cctgattgta aatatgtaat agatgtaaac atggaagtct ctccttcatt tcacgtaata
     7741 cgtgtttcac taaattcaag aaaatttgtc cgtttaaatt tgttctaaga gtacctttat
     7801 ttcaatatat taagacgaac aaacgatatt tttcaacttt tctcctatac tgcaatacta
     7861 taaaaataaa aaaggaggaa ttattatgag catttttaac attcttttaa ccatccacat
     7921 attatttggg acaatatgct taattaccgg tattgtggcg atggttgctc aaaagaagaa
     7981 aggaaaacat acggaatggg gtgaaatata tcatgcatcc tatgtggtca taacccttac
     8041 tgcaattata ttatccatta taaactggga taaaatcgca tatttatttt atatagcaat
     8101 tttctcttat gcttttgcga tttatggcta ccttgcacga aaaaaacgtt ggaaaaattg
     8161 gcttcaccac catattaaag gtatgttagg ttcttatatt ggtgccgtta ccgcactatt
     8221 agtgaacgta ggtattcata tccccataat taatttattg ccgcctatat ggttttggtt
     8281 tttacctaca ttaatcggta ttcctctcgt agccagtgta tcaaaaaaat ataaaaagcg
     8341 tagttaaaat tcattacatt ttcgttactt tctgatgatt attatgaaaa atcatgctca
     8401 atgtaatatt aataaattta aaatcgtatt gatttaagtt agtcacttag agctaaataa
     8461 aaagaatcca atttaagtct tggattcttt ttcgaatttc attttaatgt cggctctatg
     8521 tatttaatga tgttattatc tggatgacga actatcattt tcatacctgt gggtatttgt
     8581 attggttcag tgataattac accattatag tttaagatta gtttttttac ttcttggaga
     8641 ttatcaatta cgattgttac tgcaacgcgt ttcttaagtg gttcatccac ttcttctatt
     8701 aaaagtaaat ttgtaaatct aattacatgc gttccattca tttgaaattc ttgtggcttt
     8761 tcttgcctta atagtatttt gtaaaattct agatgagttt gtaagtctga aacatagagg
     8821 cgtggaacaa cagctaatac attcatccta cttacactcc tttttagttt aactagatta
     8881 atttaattat atattactcg aaaacctcta taaattatta cgttaaaaat agtatctttt
     8941 aattaagact ttaataatga atgtaaacga tcagggtaat tcgtaaatat cccgtcaact
     9001 ccccataaaa gtaacctttt catatcttgt tcattatcta cagtaaatgg atgaacttct
     9061 aatccatgtt tctttattct ctttacataa gctgcatcaa tatatttgta gttcatacca
     9121 agaccgatgc agtatgtttt atactttttt atctctgatt cagttaattg aactgctttt
     9181 ttgtaagata gtaattggac tagaggtata ttaacatata aactatgtag cttttgcaaa
     9241 ctttcttcac taaatgattg aataataact ttatcctgta tttcatagtg attgataatt
     9301 tctaataact tttcttccat tcctggatat tcatcaggtg attttgtttc aatataatag
     9361 ttcgcttcat gcccaaacac ttcaattact tcttcgagtg ttggaacttt agcattttcg
     9421 tattctctct ttgctaaatt tggatgtttt tcattaaaag aagaatctgc ctttaattgc
     9481 tttaattctt ctaatgtatg ttccttaact agtcctgtcc catttgtagt acgatttaat
     9541 gtttcatcat gcatagctac taaatgtcca tcttttgtca tttgaagatc tatttctata
     9601 taatctgctt ttaattgttg tcctaatgta taagctggta ttgtatgttc cggcgcatag
     9661 gcagaggcgc ctctatgtga aatatttttt atgtgatttg attgattaat agctttcgtg
     9721 tacttaagtt catttacttt ataataaaca aaagtaccta ttactagaaa ggcgcaaatg
     9781 attgtaacga tataaatctt tttcataatg atgtattagc tcctacatga aagcaatttg
     9841 gcttggataa tttattcaaa taaatgtcga ttttcaaata cagtcatttg tccaacagat
     9901 tgaaaaccag actttttata aatattaatc ccatcaggtg aagcttgtaa tacgcattga
     9961 gcaacgttta attcctttgc ttcttgcagc aagtaatgaa acatagtaga accaaatcct
    10021 tttcctctca tttcttcttt cgttgctata tcataaatac caatactatc ttgtgtacat
    10081 actaatgaac caaccgatat gacttcatct ttataaaacc ctaagtacag tttcatattt
    10141 tcactattcc ataaatgggc agaagcagtt tgattgtaga atgcttgtac atatttccct
    10201 tcctctgatg taccaaatag actagctaac gcttctccaa actttttaat ttgtcctgac
    10261 gaagatg
//
