LOCUS       NZ_CP047883            12109 bp    DNA     circular CON 27-JUL-2024
DEFINITION  Streptococcus mitis strain S022-V3-A4 chromosome, complete genome.
ACCESSION   NZ_CP047883
VERSION     NZ_CP047883.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus mitis
  ORGANISM  Streptococcus mitis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2086958)
  AUTHORS   Pinzauti,D., Iannelli,F., Pozzi,G. and Santoro,F.
  TITLE     Complete genome sequence of the tetracycline resistane Streptococcus
            mitis isolate S022-V3-A4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086958)
  AUTHORS   Pinzauti,D., Iannelli,F. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2020) Department of Medical Biotechnologies,
            University of Siena, Viale Bracci, 16, V lotto, I piano, Siena, SI
            53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-NOV-2018
            Assembly Method                   :: Unicycler v. NOV-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013305725.1-RS_2024_07_27
            Annotation Date                   :: 07/27/2024 01:18:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,027
            CDSs (total)                      :: 1,952
            Genes (coding)                    :: 1,893
            CDSs (with protein)               :: 1,893
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 39 of 59
            Pseudo Genes (internal stop)      :: 19 of 59
            Pseudo Genes (multiple problems)  :: 16 of 59
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:35:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 30022
            Orig. end                         :: 42131
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047883.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Francesco Santoro.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12109
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12109
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12109
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7109](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7109
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            852..1622
                     /gene="recO"
                     /locus_tag="M594_RS00165"
                     /old_locus_tag="M594_00165"
     CDS             852..1622
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /locus_tag="M594_RS00165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00165"
                     /product="DNA repair protein RecO"
                     /protein_id="WP_173875638.1"
                     /transl_table=11
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQAGKRMFFVKHAGQSK
                     LAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADASLQ
                     DNQQDAPLFAFLRKTLELMEEGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRIGQAF
                     DFSFKYGACLCPDHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPEIKQELRQF
                     MDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            1619..2611
                     /gene="plsX"
                     /locus_tag="M594_RS00170"
                     /old_locus_tag="M594_00170"
     CDS             1619..2611
                     /EC_number="2.3.1.274"
                     /GO_function="GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="plsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /locus_tag="M594_RS00170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00170"
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="WP_173875639.1"
                     /transl_table=11
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALADFSDIEVQLYGDEDKIKK
                     YLTATERVNIIHTDEKIDSDDEPTRAIRKKKNASMVLAAKAVKEGEADAVLSAGNTGAL
                     LAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSFYAK
                     NVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVADVVV
                     ADGFTGNAVLKSIEGTAMGIMGLLKTTITGGGLRAKLGALLLKDSLRGLKKQLNYSDVG
                     GAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            2617..2853
                     /locus_tag="M594_RS00175"
                     /old_locus_tag="M594_00175"
     CDS             2617..2853
                     /NRPS_PKS="Domain: PP-binding (8-73). E-value: 8.1e-12.
                     Score: 37.3. Matches aSDomain:
                     nrpspksdomains_M594_RS00175_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014476.1"
                     /locus_tag="M594_RS00175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00175"
                     /product="acyl carrier protein"
                     /protein_id="WP_000136444.1"
                     /sec_met_domain="PP-binding (E-value: 1.2e-10, bitscore:
                     38.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding_2 (E-value: 0.00013, bitscore:
                     19.0, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEFI
                     LTLEDEFNIEISDEEIDQLQSVGDVVEVVKSKE"
     aSDomain        2641..2835
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M594_RS00175_PP-binding.1"
                     /evalue="8.10E-12"
                     /label="M594_RS00175_PP-binding.1"
                     /locus_tag="M594_RS00175"
                     /protein_end="73"
                     /protein_start="8"
                     /score="37.3"
                     /tool="antismash"
                     /translation="RIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEFILTLEDEFN
                     IEISDEEIDQLQSVGDVVEV"
     misc_feature    3006..3019
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.13"
                     /tool="antismash"
     misc_feature    complement(3172..3186)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            3298..3474
                     /locus_tag="M594_RS00180"
                     /old_locus_tag="M594_00180"
     CDS             3298..3474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180827.1"
                     /locus_tag="M594_RS00180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00180"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_000180828.1"
                     /transl_table=11
                     /translation="MTNFDKMEQNFVALTEEELMNVDGGIAWEVVSVGIAIGWGIYQVG
                     EAAGKTFYYITHP"
     gene            3487..3678
                     /locus_tag="M594_RS10050"
     CDS             3487..3678
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147622.1"
                     /locus_tag="M594_RS10050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_254597153.1"
                     /transl_table=11
                     /translation="MSKKTFWIILLVITIVVTGIGLGLSAYNYYVFDRPFFNSTTKGLL
                     SAFVMSVLMIIIGILKEN"
     gene            3706..3876
                     /locus_tag="M594_RS00185"
                     /old_locus_tag="M594_00185"
     CDS             3706..3876
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519421.1"
                     /locus_tag="M594_RS00185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00185"
                     /product="H354_08695 family bacteriocin-like peptide"
                     /protein_id="WP_173875640.1"
                     /transl_table=11
                     /translation="MSNLQTIEQNYTTLSDIELQEVDGGGVLLVVGGGILAVGLLAWGA
                     YNGYQSAARGG"
     gene            3916..4095
                     /locus_tag="M594_RS00190"
                     /old_locus_tag="M594_00190"
     CDS             3916..4095
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846993.1"
                     /locus_tag="M594_RS00190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00190"
                     /product="hypothetical protein"
                     /protein_id="WP_000846988.1"
                     /transl_table=11
                     /translation="MKTFLLGFAEGYFIVSLIIYIATYLILKNPINTLFYPEIYPVLFG
                     LFYLGYKVNKKQVE"
     misc_feature    3918..3929
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 18.98"
                     /tool="antismash"
     gene            4503..4691
                     /locus_tag="M594_RS10055"
     CDS             4503..4691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269957.1"
                     /locus_tag="M594_RS10055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_254597155.1"
                     /transl_table=11
                     /translation="MKTVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVPPLF
                     SLIVLYMIDKRIRKKKK"
     gene            5001..7109
                     /gene="comA"
                     /locus_tag="M594_RS00195"
                     /old_locus_tag="M594_00195"
     CDS             5001..7109
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="M594_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00195"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_173875641.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2e-43, bitscore:
                     144.3, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1040.1,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTT
                     MDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLSRERFEEEWTGVTLFMAPSPNYKPHKDQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDLMNGVADVVVADGFTDANSIIDALASTILSIFLDVSTVVII
                     SLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEANAVLSSSIIEDINGIE
                     TIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAHLLLNVGILWMGAVLV
                     MDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRLNEVYLVASEFEEKKT
                     VEDLSLMKGDMDFKQVSYKYGYGRDVLSDINLTIPQGSKVAFVGISGSGKTTLAKMMVN
                     FYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQPYVFNGTILENLLLGAKEGTTQEDI
                     LRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARALLTDAPVLILDEATS
                     SLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLDQGKIVEEGKHADLLAQGG
                     FYAHLVNS"
     gene            7122..8471
                     /gene="comB"
                     /locus_tag="M594_RS00200"
                     /old_locus_tag="M594_00200"
     CDS             7122..8471
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="M594_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00200"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_173875642.1"
                     /transl_table=11
                     /translation="MKPEFLESAEFYNRRYHNFSSRVVVPMSLLLVFLLGFATVAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGELLVQYQEGAEGVQAEAYAS
                     QLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQNETI
                     ASQNAAASQTQTEIGTLISQTEAKIRDYQTAKSAIETGASLASENLAYSLYQSYKSQGE
                     ENPQSKAQAVAQVEAQLSQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLDQKILEAESGKKVQGNLLEKGKITASEDGVLHLNPETSDSTMVAEGALLAQL
                     YPSLEKEGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAKNQLFLDSTITSIDATATKTE
                     KGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            8641..9348
                     /gene="purC"
                     /locus_tag="M594_RS00205"
                     /old_locus_tag="M594_00205"
     CDS             8641..9348
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /locus_tag="M594_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M594_00205"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_173875643.1"
                     /transl_table=11
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVEIIPLEVVLRNYTAGSFSKRF
                     GVDEGIAFETPIVEFYYKNDDLDDPFINDEHVKFLKIADDQQIAYLKEETRRINELLKA
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLGELTD
                     VYEIVWEKLQGLK"
     misc_feature    complement(9510..9528)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.95"
                     /tool="antismash"
ORIGIN
        1 gctactttga cagctatttt ggaagaggga gacaaggtac tcttgccagc tcctgcttat
       61 ccgggttatg aaccgattgt caatctagtt ggggcagaga ttgtcgagat tgacacaact
      121 gaaaatggtt ttgtcttgac tcctgagatg ttggagaagg ccattttgga gcaaggtgat
      181 aatctcaagg cggttattct caactatcca gctaatccgt caggaattac ctatagtcgg
      241 gagcagttgg aagccttggc agctgtttta cgcaagtacg aaattttcgt tgtctgtgat
      301 gaggtttact cagaattgac ctacacaggg gaagcccatg tgtctctggg aactatgttg
      361 agagatcagg ctattattat caatggtcta tctaaatcac atgccatgac aggttggcgt
      421 ttgggtctga ttttcgctcc tgcagccttt acagctcagt tgattaagag tcaccagtac
      481 ttggttactg ccgcaaatac tatggctcaa catgctgcgg tagaagcctt gacggctggt
      541 aaaaatgatg cggaacctat gaagaaggaa tacatccagc gtcgagatta tattatcgag
      601 aaaatgacag ctcttggttt tgagattatc aaaccagacg gtgccttcta tatctttgct
      661 aagattccag cgggctacaa tcaagactcc tttgcttttt tgaaggattt tgctcagaag
      721 aaggctgttg cctttatccc tggtgctgcc tttgggcgtt acggggaagg ctatgtgcgt
      781 ctgtcttatg cggctagcat ggagactatc aaagaagcca tgaaacgact tgaggagtac
      841 ataagagaag catgattcag tctatcacga gtcaaggctt ggtgctctac aatcgtaact
      901 ttcgtgagga tgacaagctg gtcaaaattt ttaccgagca ggcgggcaag cgcatgtttt
      961 tcgtcaaaca cgctggccag tccaaactag ctccggttat tcagcccttg gtgttggcac
     1021 ggtttctctt acgaatcaat gatgacggcc ttagctacat cgaggactac catgaggtga
     1081 tgacctttcc caagattaat agtgatctct ttgtcatggc ctatgcgact tatgttgcgg
     1141 ctctggccga tgctagtttg caggataatc agcaggatgc tcccttattt gccttcttga
     1201 gaaagactct agagttgatg gaagagggtt tagattatca agttttgacc aatatttttg
     1261 aaattcaaat cttgacccga tttggtatta gtctcaattt taatgagtgt gtcttttgcc
     1321 atcggattgg tcaggctttt gacttttctt tcaaatatgg agcctgcctc tgtccagatc
     1381 attatcatga ggatgagaga cgttgccatc tcaatcccaa tatcccctat ctgctcaatc
     1441 aatttcaagc tattgatttt gagaccttgg agaccatttc gctcaagcct gaaatcaagc
     1501 aagagttacg ccaatttatg gatcaactct acgaagagta cgttgggatt cacctaaaat
     1561 caaagaaatt tattgattcc ctagcagact ggggacaatt actaaaagag gaaaagaaat
     1621 gaaaaaaatc gcagtagatg ccatgggggg cgattacgca cctcaagcca tcgttgaggg
     1681 tgtcaatcaa gccctagctg acttttcaga tatcgaggtt caactctacg gagatgaaga
     1741 taaaatcaag aaatatttaa cagcaacaga gcgcgtcaac attatccata cagatgagaa
     1801 gattgattca gacgatgaac ctacgagagc tattcggaag aagaaaaatg ccagtatggt
     1861 attggcggcc aaggctgtca aagaaggcga agcagacgct gtgctctccg ctgggaatac
     1921 aggtgctttg ttagcagctg gattcttcat cgtgggtcgt atcaagaata tcgatcgacc
     1981 agggctcatg tcgaccttgc caactgtaga tggaaaaggt tttgacatgc tagacctcgg
     2041 tgcaaatgca gaaaatacag cccagcacct ccatcaatat gctgttctag gttccttcta
     2101 tgccaagaat gttcgtggca ttgcgcaacc acgtgttggt ttgctcaaca acggaacaga
     2161 gagtagtaag ggggacccgc ttcgtaagga aacttatgaa ttattagtgg ctgatgaaag
     2221 tttgaacttt atcggaaatg tggaagcgcg tgatttgatg aatggcgttg cggatgttgt
     2281 cgtggcagat ggtttcacgg gaaacgctgt gctcaaatct attgaaggga cagctatggg
     2341 aatcatgggc ttgctcaaga ccactattac aggtggtggt cttcgagcga aactaggcgc
     2401 cctccttctc aaggacagcc tcagaggttt gaaaaaacag ctcaactact cagatgttgg
     2461 tggagcagtc ttgtttggtg tcaaggcacc ggttgtcaag actcatggct caagcgatgc
     2521 caaggctgtt tatagtacga ttcgtcagat tcgtaccatg ctagaaacag acgtcgttgc
     2581 ccaaactgca cgtgaatttt caggagaata aaatagatga cagaaaaaga aatttttgac
     2641 cgtattgtga ccattatcca agagcgacag ggagaggact ttgtcgtgac agaatccttg
     2701 agtctgaaag atgatttgga tgctgactct gttgacttga tggagtttat cttgacactg
     2761 gaagatgaat ttaatatcga aatcagcgat gaggaaattg accaactgca aagtgtagga
     2821 gatgtggtag aagtcgtcaa aagtaaagaa tagtaagaag caacatgcaa gtcgtgttgc
     2881 ttttttattg tcaggaaaga taaaaaaatt agaactttta gtgggaatta cgaatagata
     2941 gataaggaag aaaaaggagg agtgtttatg tatgtgactg gtttttatcc gtggttcatt
     3001 aaactatttt tcaaataaaa tttaattaat tgtacatttt cgaattattt ttcgaataaa
     3061 taagtgagaa gaaaaggaag gagattacgc gatgtattta ccaatcttat tgccatggtt
     3121 tatcaaatgg tatttaaaat aaatgaaatt tacctgttca ttttaaatca cttctcaaat
     3181 agataagtga gacgaaaagg aaggaaaagg aattcagtaa tttatctatt ctggttttta
     3241 aaatggttta agagccagaa atcgtaaact aaaaaataga aataaaggag aaaaaagatg
     3301 acaaattttg acaaaatgga acagaacttt gtagctctta cagaagaaga gttgatgaat
     3361 gtagatgggg gaattgcttg ggaggtagtc tccgtgggta ttgcgattgg ctggggaatt
     3421 taccaagttg gtgaagctgc tggtaaaaca ttctactata ttactcatcc atagtttttg
     3481 ataaatatga gcaagaaaac attttggatt atactactcg ttattacaat tgtagtgact
     3541 ggcattggtt taggattgtc tgcatataat tattacgttt ttgatagacc ttttttcaac
     3601 agtactacta aaggcctgtt gtctgcattt gttatgagtg tattgatgat tattatcggt
     3661 atattaaaag aaaactaatt tttataatta aaatggagga tatatatgtc aaatttacaa
     3721 actattgaac aaaattatac tacgttaagt gatattgaat tgcaagaagt tgatggtgga
     3781 ggagtactac ttgtggttgg tggtggtatt ttggctgtcg gactgttagc ttggggtgcg
     3841 tataatggtt atcagtcggc agcacgaggt ggataaaaag aaggtagatt gttttttagt
     3901 aaataggagt ctgccatgaa aacgttttta ttaggttttg ctgagggtta ttttatcgta
     3961 tcgctcataa tctacatcgc aacgtatttg attttgaaaa atccgattaa taccctattt
     4021 tatccagaaa tttaccctgt tttatttggg ctcttttatc taggatataa ggtcaataaa
     4081 aagcaagtag agtgagcagc taagaatgac ttgttagtct gcgctgaaaa tggttagggt
     4141 agaatctaag aatgcatcac agtggagttt aaaatgagta actatgaatc gtaatttaga
     4201 acggtgttat ctattctgac tatgaacaga tcataccaga ggtggctcag aaatagcagg
     4261 gacattagaa attgaagtaa tgaataggat gtcacaaatg ttactatcaa tgatttattt
     4321 gttccaagct tgcctagggt gtcagtaaaa aatcaatttc ctttcatatc atatttttag
     4381 taggtagggc gcttgttctg cctatttttt tgccaagaaa gtgcagttga ggtgtttatt
     4441 tggagtttta catatatttt tgataaaata ggtatagaaa ttaggaaagt tgaagaaaag
     4501 aaatgaagac ggttttaaaa tattttttag tatttgtttt tctatttttg atgaatattt
     4561 tcatctttaa gatacttgca actctgggat ttcagttgat aatgagtgaa aagagttata
     4621 ttgtaccacc gctgttttcg cttatcgttt tatatatgat tgacaaaaga attaggaaga
     4681 aaaagaaata aatatatatt ttaggcatga cgtttgttct gcctattttt ttatctcaaa
     4741 agtgcagttg ggagggagat aggcgcattt ggggaggaag tccagttttt gtttggtgat
     4801 tggggtaaga tagttgttat cagatgagtt tatactcttc gaaaatcaaa ttcaaaccac
     4861 gtcaacgtcg ccttgccgta tatatgtgac tgacttcgtc agtcttatat acaacctcaa
     4921 aacagtgttt tgagcagcct gtggctagtt tcctagtttg ctctttgatt ttcattgagt
     4981 attaggaaaa ggagatgaat atgaaatttg ggaaacgtca ctatcgtccg caggtggatc
     5041 agatggactg cggtgtagct tcattagcca tggtttttgg ctactatggt agctattatt
     5101 ctttggctca cttgcgagaa ttggccaaga caaccatgga tgggacaacg gctttgggct
     5161 tggtcaaggt ggcagaggaa attggttttg agacgcgagc aattaaggcg gatatgacgc
     5221 tctttgactt gccagatttg acttttcctt ttgttgccca tgtgcttaag gaagggaaac
     5281 tgctccacta ctatgtggtg actgggcagg ataaggatag cattcatatt gccgatccag
     5341 atcccggggt gaagttgact aaactgtcac gtgagcgttt tgaggaagaa tggacaggag
     5401 tgactctttt tatggcacct agtccaaact ataagcctca taaggatcaa aaaaatggcc
     5461 ttctctcttt tatccctata ttagtgaagc agcgtggctt gattgctaat atcgttttgg
     5521 caacactctt ggtaaccttg attaacattg tgggttctta ttatctgcag tctatcattg
     5581 atacctatgt gccagatcag atgcgttcga cgctggggat tatttctatt gggctggtca
     5641 tcgtctatat cctccagcag atcttgtctt acgctcagga gtatctcttg cttgttttgg
     5701 ggcaacgctt gtcgattgat ttgatgaatg gcgttgcgga tgttgtcgtg gcagatggtt
     5761 tcacggatgc taatagtatc atcgatgcgc tagcttcgac catcctttcg attttcctag
     5821 atgtgtcaac ggttgtcatt atttctcttg ttttattttc acaaaatacc aatctctttt
     5881 tcatgacctt attggcactt cctatctaca cagtgattat ctttgctttt atgaagccgt
     5941 ttgaaaagat gaatcgggac accatggaag ccaatgcggt gctgtcttct tctatcattg
     6001 aggacatcaa cggtattgag actatcaagt ctttgaccag tgaaagtcag cgttaccaaa
     6061 agattgacaa ggaatttgtg gattatctga agaaatcctt tacctatagt cgggcagaga
     6121 gtcagcaaaa ggctctgaaa aaagtggccc atctcttgct caatgtcggc attctctgga
     6181 tgggggctgt tctggtcatg gatggcaaga tgagtttggg gcagttgatt acctataata
     6241 ccttgcttgt ttactttacc aatcctttgg aaaatatcat caatctgcaa accaagcttc
     6301 agacagcgca ggttgccaat aaccgtttaa atgaagtgta tctagtagct tcggagtttg
     6361 aggagaagaa aacggttgag gatttgagtt tgatgaaggg agatatggac tttaagcagg
     6421 tttcctacaa gtatggctat ggtcgagacg tcttgtcgga catcaatttg actattccgc
     6481 agggttctaa ggtggctttt gtggggattt cggggtcagg taagacgaca ttggccaaga
     6541 tgatggttaa tttttacgat ccaagtcagg gagagattag tctgggtggt gtcaatctca
     6601 atcagattga taaaaaagcc ctgcgccagt atatcaacta tctgcctcaa cagccctatg
     6661 tctttaacgg aacgattttg gagaatctac ttttgggagc caaggagggg acgactcagg
     6721 aggatatttt acgggcagtc gaattggcag agattcggga ggatatcgag cgcatgccac
     6781 tgaattacca gacagaattg acttcggatg gagcaggaat ttcaggtggg caacggcagc
     6841 gaattgcttt ggcacgtgct ctcttgacag atgcaccggt cttgattttg gatgaggcga
     6901 ccagcagttt ggatattttg acagagaagc gaattgtcga taatctcatg gctttagaca
     6961 agaccttgat tttcatcgcc caccgtttga ctattgctga gcggacagag aaggttgttg
     7021 tcttggatca gggcaagatt gttgaagaag gaaagcatgc tgatttgctt gcacagggtg
     7081 gcttttacgc ccatttggtc aatagttaga aagaggagag gatgaaacca gaatttttag
     7141 aaagtgcgga gttttataat cgacgttacc ataatttttc cagtcgggtg gttgtaccca
     7201 tgtcccttct gctcgtgttt ttgcttggct ttgcaactgt tgcagagaag gagatgagtt
     7261 tgtccaccag agcgactgtt gagcctagtc gtatcctggc aaatatccag tcaactagca
     7321 acaatcgcat tcttgttaat catttggaag aaaataagct ggttaaaaag ggagaactgt
     7381 tggttcaata tcaagaaggg gcagagggtg tccaagcgga ggcctatgct agtcagttgg
     7441 acatgctcaa ggatcaaaaa aagcaattgg agtatttgca gaagagccta caagaagggg
     7501 agaaccactt tccagaggag gataagtttg gctaccaagc cacctttcgt gactacatca
     7561 gccaagcagg tagtcttagg gctagtacat cgcaacaaaa tgagaccatc gcgtcccaga
     7621 atgcagcagc tagtcaaacc caaaccgaaa tcggcaccct catcagtcaa acagaggcta
     7681 aaattcgcga ttaccagaca gctaagtcag ctattgaaac aggtgcttcc ttggccagtg
     7741 agaatctagc ctactctctc taccagtcct acaagtctca gggcgaggaa aatccgcaat
     7801 ctaaggcaca agcagttgcg caggttgaag cacagctttc tcagttagaa tctagtcttg
     7861 ctacttaccg tgtccagtat gcaggttcag gtacccagca agcctatgcg tcaggtttaa
     7921 gcagtcaatt ggaatctctt aaatcacaac acttggctaa ggttggtcag gaattgaccc
     7981 ttctagacca gaaaattttg gaggcagaat cgggtaagaa ggttcaggga aatcttttag
     8041 aaaaggggaa aattacggcg agtgaggatg gggtgcttca ccttaatcct gagactagtg
     8101 attctactat ggtagcagaa ggtgccttat tagctcaact ctatccgtcc ttggaaaaag
     8161 aagggaaagc caaactcaca gcttatctta gttcaaagga tgtagcaagg atcaaggtcg
     8221 gtgattctgt tcgttatact acgactcatg atgccaagaa tcaacttttc ctagattcta
     8281 ctattacaag tattgatgcg acagctacta agactgaaaa agggaatttc tttaaaatcg
     8341 aggcggagac taatctaact tcggagcagg ctgaaaaact tcggtacggg gtggaaggtc
     8401 gcctgcagat gattacgggc aagaaaagtt acctacgtta ttatttagat caatttttga
     8461 acaaagagta atgttcgtat ttttagagtt aaatgatttt aaaactgtga gaaagatttt
     8521 tcttgcagtt ttttctttat gatttttgaa gttactaaat tatttattcg gttaaattct
     8581 tgtaatttta ggttttttat ggtagaatgt gctcaagtaa tacgaaaggc gaactttaaa
     8641 atgtcaaaac aattgatcta ttcgggaaaa gctaaagata tctatacaac tgaggatgaa
     8701 aatcttatta tttcaactta caaggaccag gcgactgctt tcaatggtgt taagaaggag
     8761 cagattgcag gcaagggagt attgaataat cagatttcat cttttatttt tgagaaatta
     8821 aatgcggctg gtgtggcgac tcactttgtg gagaaacttt cagatacgga acaactcaat
     8881 aaaaaggtgg aaatcattcc tttggaagtc gtgcttcgca attatactgc tggctccttt
     8941 tcaaaacgtt ttggtgtaga cgaaggaatc gcatttgaga ctccaattgt agaattttac
     9001 tataaaaatg atgatttgga tgatcccttt atcaatgacg agcatgtgaa attccttaaa
     9061 attgcggatg accagcagat tgcttacttg aaggaagaaa cgcgtcgtat caatgagctc
     9121 ttgaaagctt ggtttgctga gattggtctt aaattgattg actttaagtt agagtttggt
     9181 tttgacaagg atggcaagat tatcttggca gacgaatttt caccagataa ctgccgactt
     9241 tgggacgctg acggtaacca catggataag gatgttttcc gtagaggtct cggagaatta
     9301 acagacgttt acgagattgt ctgggagaaa ttgcagggtt tgaaataatc tgtttgcaag
     9361 actctcaagg tcgttgggaa cattgcaaga gctgaaataa aggaataaga attgatggat
     9421 aaacgtattt ttgttgagaa aaaggctgat tttcaggtca agtcagagag tttggtgaga
     9481 gaactccagc ataacttggg actatcaact ttgaaaagta ttcgcatcgt gcaagtttat
     9541 gatgtctttg atttggcaga gaacttgttt gcgcctgcag aaaaacatat cttctctgag
     9601 caggtgacag accatgtatt tgacgaatcg gctgtgcagg ctgatcttgc taactatgct
     9661 ttctttgcca ttgaaagttt gccaggacag tttgaccaac gtgcagcttc ttcacaggaa
     9721 gccttgcttt tgctgggaag ttcgagtgac gtgacggtca acacagcaca attgtacttg
     9781 gttaataaag atattgatgc gactgagttg gaagcagtca agaactattt gctcaaccca
     9841 gttgattctc gtttcaagga catcacgaca gggattgcca agcaggaatt ttcagagtca
     9901 gacaagacta ttccaaaatt gactttcttt gaaaactata cggcagaaga ctttgcccgc
     9961 tacaaggccg agcaagggat ggcaatggaa gtggatgatt tgctctttat ccaagattac
    10021 ttcaagtcaa tcgggcgcgt gccgactgag acggagctca aggttttgga cacttactgg
    10081 tctgaccact gccgtcacac gacttttgag accgagttga aacagattga tttctcagct
    10141 tctaaattcc aaaagcaatt gcaagcgact tatgacaagt atattgccat gcgtgaggaa
    10201 ctaggtcggt ctgaaaagcc acaaaccttg atggatatgg cgactatttt cggtcgttat
    10261 gagcgtgcta atgggcgttt ggacgacatg gaagtgtcag acgaaatcaa tgcctgctcg
    10321 gttgaaatcg aagtggacgt tgatggtgtc aaggaaccat ggctccttat gttcaaaaac
    10381 gaaacccaca accacccaac ggaaattgaa ccatttggtg gcgcggctac ttgtatcggt
    10441 ggggctattc gtgacccatt gtcaggtcgt tcttatgtct accaagctat gcgtatctca
    10501 ggtgctggag atattacagc accgatttcg gaaactcgcg ctgggaaatt gccacaacaa
    10561 gtcatttcta aaacagcagc tcatggttat tcttcatacg ggaatcagat tgggcttgcg
    10621 acgacctatg ttcgtgagta cttccaccca ggctttgtag ctaaacgcat ggagttgggt
    10681 gccgttgttg gtgcagctcc aaagggcaat gttgtccgtg aaaaaccgga agcgggagat
    10741 gtcattatcc tcctcggtgg taaaactggc cgtgatggtg tcggtggtgc gacgggctct
    10801 tctaaggttc aaacggttga gtctgtggaa actgctggag ctgaggttca aaaggggaat
    10861 gccatagaag aacgtaagat tcagcgcctc ttccgtaatg gtgatgtcac tcgtctcatc
    10921 aagaaatcca acgactttgg tgctggtggt gtctgtgtag ccattggtga attggcagac
    10981 ggtcttgaaa tcgatctcaa caaggttcct cttaaatacc aaggcttgaa tggtaccgaa
    11041 attgccatct ctgaatcaca agaacggatg gctgtcgtgg ttcgtcctga ggatgtagat
    11101 gcctttgttg aagaatgtaa caaagagaat attgacgctg ttgtggtggc gacagtgact
    11161 gaaaaaccaa atctagtcat gcactggaat ggtgaaacca ttgtcgactt gggacgtcgt
    11221 ttccttgaca ccaatggtgt gcgtgtggtc gtcgatgcta aggttgtgga caaggatgtc
    11281 aatcttccag aagaacgtca aacatctgct aacacacttg aagttgatac ccttgcggtt
    11341 ctatctgatt tgaatcatgc gagtcaaaaa ggattacaga ctatctttga ctgctctgtt
    11401 ggacgttcaa cggttaatca cccacttggt ggtcgttacc aactcacacc aactgaggca
    11461 tctgtgcaga aattaccagt tcaacacggt gtgactcata ctgcgtcagt catggctcaa
    11521 ggattcaacc catatgtagc tgaatggtct ccataccacg gtgctgccta tgcggtgatc
    11581 gaagcaactg ctcgattggt tgctacgggt gccaactggt ctaaggctcg cttctcttat
    11641 caagagtact tcgagcgcat ggacaaacaa gctgagcgtt ttggtcagcc agtggcagct
    11701 ctcctaggct ctatcgaggc acaaattcag cttggtttgc catctatcgg tggtaaggac
    11761 tctatgtctg gtacttttga agaattgaca gtgccaccaa ccttggtcgc ctttggggta
    11821 acaacagcag atagccggaa ggtgctttct cctgagttca aggttgctgg tgaaaatatc
    11881 tactacatcc caggtcaagc cctctctgca gaaattgatt ttgacttgat taagtctaac
    11941 tttgctcagt ttgaagccct tcaaaaggct cacaaagtga catctgcatc agctgtcaaa
    12001 tatggtggtg ttgttgaaag tttggccctt gctacctttg gaaatcatat cggtgcagaa
    12061 gtaaccttgc ctgaacttga aacggctttg acagctcaat taggcggat
//
