LOCUS       NZ_CP053892            10801 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Actinomadura verrucosospora strain NRRL:B18236 chromosome.
ACCESSION   NZ_CP053892
VERSION     NZ_CP053892.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M.
  TITLE     Actinomadura verrucosospora NRRL-B18236 (PFL_A860) Genome sequencing
            and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2016
            Assembly Method                   :: Newbler v. 3.0
            Assembly Name                     :: A860_AS2.mpe1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013211575.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,277
            CDSs (total)                      :: 9,181
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 96
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 18 of 71
            Pseudo Genes (incomplete)         :: 57 of 71
            Pseudo Genes (internal stop)      :: 2 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:15:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9576684
            Orig. end                         :: 9587485
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Peter Leadlay,
            NRRL-B18236 (patent collection, not in online catalogue).
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5801)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            416..994
                     /locus_tag="ACTIVE_RS43270"
                     /old_locus_tag="ACTIVE_8671"
     CDS             416..994
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626397.1"
                     /locus_tag="ACTIVE_RS43270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8671"
                     /product="GAF domain-containing protein"
                     /protein_id="WP_246342543.1"
                     /transl_table=11
                     /translation="MIHDPLYEPSGHLLTPVDPDAPERAVRLRELRLGGPDAEFDAFAR
                     DLAEAARHLAGTDLPPFAMVNFVTDQQYLGGLYAPQAPGADPADAVELGREVPLDHGYC
                     PHVVVRRKALVLDDVCDYPRFAGNPVVDKLGIRTYVGAPLIDRTGTVLGTICVIDREPR
                     PWGHAGLDLIKERAADLMQVIRRREERQG"
     gene            complement(1011..2531)
                     /locus_tag="ACTIVE_RS43275"
                     /old_locus_tag="ACTIVE_8672"
     CDS             complement(1011..2531)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /locus_tag="ACTIVE_RS43275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8672"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_173100369.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.2e-10, bitscore:
                     41.0, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.5e-12, bitscore:
                     45.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.8e-17, bitscore:
                     63.0, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNDLLSLSTNAFIAGIEAAARRFDTATYPQQNLPADDWTLLTRA
                     GVLLPTLPKEYGGRDDHVEMCRAVETVSEWNLPVGMYVTVITAVALRPIVLWAREEAKQ
                     EVLPLYAGGDPAICGFASTEPGCGSAMSGMRTTFEEVEGGYRIRGRKHWQAFSISAHWW
                     LVSAKNDEDGRSYGYFIVKRSEGFRTVQPYEPLGLKAIDYGLNEIDAVVPRHRRIPAES
                     KNLRPMVEMLMASRAMMAAMGCGFLRRISREAHAYADRRKIGPGPLSDIRFVRYRLAAI
                     DASYTVCAALNHYLQNDLDMKESMIGAFPAVQAIKTVATERMLSSAHHYQQLAGGEGYR
                     CGSPTNISGQAFLDARVYTVFDGTNDLLSQQLAEHCLSRTAGRPLSRFLAGWPLTAAAV
                     AAHRLDLGFLDRELRQEHRVLAGRVIGYAFAIGQVMRWAAETGADPRRARAAIEFLKAD
                     IARVAGEFRLLSTGALGIDEGAAPPADATASRSVPVAPGAWPGLPHHP"
     misc_feature    complement(2510..2525)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.46"
                     /tool="antismash"
     gene            complement(2874..4448)
                     /locus_tag="ACTIVE_RS43280"
                     /old_locus_tag="ACTIVE_8673"
     CDS             complement(2874..4448)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /locus_tag="ACTIVE_RS43280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8673"
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="WP_173100370.1"
                     /transl_table=11
                     /translation="MTRPLLAAALCAASVTALAAPATAAARTSALPLGPSSLHEQRDTR
                     QLAPGVTLTTIVRGEKSAADVWTLHVRLAVPGNTSAVIAPKATAEDAARRLESAGFHPR
                     VEAVDGPRFADVRAGRVGYVVRVGRYATEADAKKDLPALNAAGFSAGTSYTAQDGTAAT
                     GPWRLHVISVDPGRFGGRVVSSVGGTLTATDKVSDLVRTSGAVAGVNGTYFTAGGKGGS
                     AGLHVLNGELTSEANNGRTALILPQSGRGARFAQLSTVLRVRAQDRAGRELDGVNRLPG
                     LVDNCGGVGGDQPTERPQHDVTCTDPDELVAFTPVYGASSPAGDGAEAVLDASGRVTEL
                     RPARGTAIPAGGRTVQAIGTAADWLRDHARPGTRLAISEQVLDRGRPVRLDARTSILGA
                     GPQLVRDGRAWVDAYADGLVHTGDDQSFYYNWVLRRNPRTMVGVDRRGRLLMVAADGRA
                     PGFSEGLSIEEAAKVMRSLGAVQAMNFDGGGSTTMATAGGALVNRPSDGTGERSVGDGI
                     LLLPGRR"
     gene            4552..4986
                     /locus_tag="ACTIVE_RS43285"
                     /old_locus_tag="ACTIVE_8674"
     CDS             4552..4986
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /locus_tag="ACTIVE_RS43285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8674"
                     /product="acyl-CoA thioesterase"
                     /protein_id="WP_173100371.1"
                     /sec_met_domain="4HBT_2 (E-value: 8.5e-17, bitscore: 60.8,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAEPLHAVSFRLSYGDCDPAGIVYYANYQRWMERTHTEWWYLRG
                     LRFDELPARLGVAVVTRASTAEYERTITLFDVVECRLFAERLGRSSFAVRGDFVTEDGD
                     RACTTALTLVCVDAADPRRTVPVPEPMRLALGAGNAALSG"
     gene            complement(5001..5801)
                     /locus_tag="ACTIVE_RS43290"
                     /old_locus_tag="ACTIVE_8675"
     CDS             complement(5001..5801)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083253.1"
                     /locus_tag="ACTIVE_RS43290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8675"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_173100372.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1e-93, bitscore:
                     312.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISDAAWTDLEAEVRRTFTRHLAGRRVVDLVGPAGP
                     ALSAVGTGHVDPLAPPAEGVLAHRRRAQPIIELRVPFTVARQDVDDVERGALDPDWQPA
                     KDAAKKIAFAEDHAVFHGSEAAGITGIAPGSSNPALPLPADAREVPDAVAKALSALRLA
                     GVGGPYSLLLSADAYTAVSETTDHGYPVRHHLARLLGDGEIIWAPALDGALLLSTRGGD
                     YELHLGQDLSIGYLSHDAEHVRLYLQETLTFLAVTSESSVPLTA"
     gene            complement(5798..6850)
                     /locus_tag="ACTIVE_RS43295"
                     /old_locus_tag="ACTIVE_8676"
     CDS             complement(5798..6850)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083254.1"
                     /locus_tag="ACTIVE_RS43295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8676"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_173100373.1"
                     /transl_table=11
                     /translation="MPQQGQPQDVLAPPATAAIFLVVTVRPGGEDTVRGLLPDIAGLTR
                     AVGFRSPEDGLSCVTGIGAHLWDRLYGPPRPAGLHPFRTLAGAVHTAVSTPGDLLFHLR
                     ARRPDLCFELARQLMTRLAGHADVADEVHGFRYFDERDLLGFVDGTESPSGPDGVVAVT
                     IGDDDPAFAGGSYVVVQKYLHDMRGWNALPVEAQELAMGRRKLSDIELPDAAKPPNSHV
                     ALNTIVDPDGTQHQIVRDNMPFGSIGADEFGTYFIGYVGRVDVIERMLENMFIGDPPGN
                     HDRILDFSTAVTGGLFFVPTADFLDDPSPRLALASATAAAEPDPQPPPGGGPAADGSLG
                     IGSLRRNGTP"
     gene            7094..7303
                     /locus_tag="ACTIVE_RS43300"
                     /old_locus_tag="ACTIVE_8677"
     CDS             7094..7303
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573914.1"
                     /locus_tag="ACTIVE_RS43300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8677"
                     /product="DUF2945 domain-containing protein"
                     /protein_id="WP_173100374.1"
                     /transl_table=11
                     /translation="MAEQFSVGDHVRWNSEAGYVEGVIIRKHTHDVEYKGHMRRCSEDD
                     PQYEIKSDRTDHVAMHKGPALTKL"
     gene            7326..7958
                     /locus_tag="ACTIVE_RS43305"
                     /old_locus_tag="ACTIVE_8678"
     CDS             7326..7958
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /locus_tag="ACTIVE_RS43305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8678"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_173100375.1"
                     /transl_table=11
                     /translation="MPVPPPSVPAQRWISVAAGAVVCALVCVLANVPVGLLSGIAAAAA
                     VFVLTGVAAVWPMTAGSTRRHARREGFRPLVAESVVVTAAIGSLVGVAVLLAVGSSGTR
                     SAAAAVGLFSVFMSWGMLHLMYAVRYAHLYYGEPAGGIDFNSEESPSYRDFLYFSYNLG
                     MTYQVSDTDVSSTAIRAVVLRHTLLSYLYGTVILAATINLVAGIISS"
     gene            complement(8404..8622)
                     /locus_tag="ACTIVE_RS47005"
                     /old_locus_tag="ACTIVE_8679"
     CDS             complement(8404..8622)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTIVE_RS47005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ACTIVE_8679"
                     /product="hypothetical protein"
                     /protein_id="WP_216858155.1"
                     /transl_table=11
                     /translation="MLDRLDGPNPPRLVCIDPRRTKVAERATVHLPIRNGTDLALMRRA
                     GRRHRPRRGDLRHGRAAGVDGRAASGR"
     gene            complement(8891..9994)
                     /locus_tag="ACTIVE_RS43315"
                     /old_locus_tag="ACTIVE_8680"
     CDS             complement(8891..9994)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /locus_tag="ACTIVE_RS43315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8680"
                     /product="tellurite resistance/C4-dicarboxylate transporter
                     family protein"
                     /protein_id="WP_173100376.1"
                     /transl_table=11
                     /translation="MNRVQRQVAGLHPGYAALVMATGIVSTGLELFGWDALSDALLGVA
                     AAALVVLAAAYVWRAVAYPRRLLADARNPSKGFGYFSLVAAPNVVGVRLALDHHVLTST
                     VLALVSVPIWLVLTYGIPGVLIVGHRDDPVLPGINGSWFMWVVGTQSLAVSAATVAGAR
                     HGWAGALAPLAVALWGIGVVLYLMLAGLVTARLLDEPVTPHALSPTYWVYMGATAITVL
                     AAAKILALPAGLPVLASTGQVVSGLAFLLWAFGTWWIPLLLFFAVWRHLVRGAPVSYEP
                     TLWSMVFPLGMYAAATASYGRARGLGFMVDIARVEVWAGFAAWLSVAVAMGAALLQPPP
                     AQPDGALGPADRGGPPRSEERKADDGR"
     misc_feature    complement(9980..9993)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(10049..10330)
                     /locus_tag="ACTIVE_RS43320"
                     /old_locus_tag="ACTIVE_8681"
     CDS             complement(10049..10330)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTIVE_RS43320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ACTIVE_8681"
                     /product="hypothetical protein"
                     /protein_id="WP_173100377.1"
                     /transl_table=11
                     /translation="MSTPMYTTDRIPDDQEGVTTLGIRAGQPGSSPESAFTNLERASAD
                     LRPDALLGVCLLAVPNVLGRTDFTTTDVMRIHTETQYVAYGTCVRFET"
     misc_feature    complement(10764..10782)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.84"
                     /tool="antismash"
ORIGIN
        1 cgcgctcgac ttcggccgga tcacgatcga gcagcacggc atcgtgctgt acctgttcgg
       61 cacccccggc cagcagcgct tctggttcat gtgggacgag ctgtccgcgg gcgcgctcgg
      121 cgccatcgtg ctcgccgaca cccgccggct ggaggactgc ttcgacgccg tcgacttctt
      181 cgagaaccgc gggacccgct tcctcgtcgc ggtcaacgag ttcgacggcg cgttccgcta
      241 cgccccggac gaggtccgcg gcgccctcgg gctcggcccg cgggtgccga tcgtgctgtg
      301 cgacgcgcgg cacggcggct ccgcgacgtc ggtgctgcgg gcgctgatcc gccatctgct
      361 cgtcgccgtc ccgtccgcct ccgcctgacg caagtcccgc ctcccaggag cccccgtgat
      421 ccacgacccg ctgtacgagc cgtccggcca cctgctgacg cccgtcgacc cggacgcgcc
      481 cgagcgcgcg gtgcggctgc gggagctgcg gctcgggggg ccggacgccg agttcgacgc
      541 gttcgcccgc gacctcgccg aggccgcgcg gcacctcgcc ggcaccgacc tgcccccgtt
      601 cgcgatggtc aacttcgtca ccgaccagca gtacctcggc ggcctgtacg cgccgcaggc
      661 gccgggcgcc gacccggccg acgcggtcga gctgggccgg gaggtgccgc tcgaccacgg
      721 gtactgcccc cacgtcgtcg tgcgccgcaa ggcgctggtc ctggacgacg tgtgcgacta
      781 cccgcgcttc gccgggaacc cggtcgtcga caagctcggc atccgcacct acgtcggcgc
      841 ccccctcatc gaccggacgg ggacggtgct cggcacgatc tgcgtcatcg accgcgagcc
      901 gcgcccgtgg ggccacgccg ggctcgacct catcaaggag cgggcggcgg acctgatgca
      961 ggtgatccgc cggcgtgagg aacgccaggg ctgacggggc gctcccgcgg tcacggatgg
     1021 tgcggcaggc ccggccacgc ccccggcgcc acggggaccg agcgggacgc cgtcgcgtcc
     1081 gccgggggag cggcgccctc gtcgatgccg agcgcgccgg tggacagcag ccggaactcg
     1141 ccggcgacgc gggcgatgtc ggccttgagg aactcgatcg ccgcgcgggc ccgccgcggg
     1201 tcggcgccgg tctccgcggc ccaccgcatg acctggccga tcgcgaaggc gtagccgatc
     1261 acgcggccgg cgaggacgcg gtgctcctgg cgcagctcgc ggtcgaggaa gccgaggtcg
     1321 agccggtgcg cggcgaccgc ggccgcggtg agcggccagc cggcgaggaa ccggctgagc
     1381 ggccgccccg ccgtccggga caggcagtgc tcggcgagct gctggctgag cagatcgttg
     1441 gtgccgtcga agacggtgta gacgcgcgcg tcgaggaacg cctggccgga gatgttggtc
     1501 ggcgacccgc agcggtagcc ctccccgccg gcgagctgct ggtaatggtg cgcggaactg
     1561 agcatccgct cggtggcgac cgtcttgatc gcctggacgg cgggaaaagc gccgatcatg
     1621 ctctccttca tgtccaggtc gttctgcagg taatggttga gcgccgcgca tacggtatag
     1681 gacgcgtcga tcgcggccag cctgtaccgg acgaaccgga tatccgaaag cggacccggg
     1741 ccgatcttgc ggcgatcggc gtaggcgtgc gcctcgcggc tgatacggcg caggaatccg
     1801 cagcccatcg ccgccatcat cgcccgcgac gccatgagca tttccaccat cggccgcaga
     1861 ttcttgctct ccgcagggat gcgccggtgc cgcgggacga ccgcgtcgat ctcgttgagc
     1921 ccgtagtcga tcgccttcag accgagcggt tcgtagggct ggacggtgcg gaatccctcg
     1981 ctgcgcttga cgatgaaata gccgtaactg cggccgtcct cgtcgttctt cgcgctgacc
     2041 agccaccagt gggcgctgat gctgaacgcc tgccagtgct tgcggccgcg gatccggtaa
     2101 ccgccctcga cctcctcgaa cgtggtgcgc atgcccgaca tcgccgagcc gcagcccggc
     2161 tcggtggagg cgaatccgca gatcgccggg tcgccgccgg cgtacagcgg cagcacctcc
     2221 tgcttggcct cctcgcgggc ccacagcacg atggggcgca gggccaccgc cgtgatcacc
     2281 gtgacgtaca tgccgaccgg caggttccat tcggagacgg tctcgacggc gcggcacatc
     2341 tcgacgtggt cgtcgcgtcc gccgtactcc ttgggcagcg tcggcagcag caccccggcg
     2401 cgggtgagca gggtccagtc gtcggcgggg aggttctgct gcggataggt cgcggtgtcg
     2461 aatctgcggg cggccgcctc gatgccggcg atgaacgcgt tcgtcgacag agacagaaga
     2521 tcattcatca tggcgtcggc cctcattggt cgatctgtcg gtgctggaag tcgtcgtccg
     2581 gcggtcaggt gcacgcggcc gccctgtttt ccggcgttgc ggcggaccat tatcggccgc
     2641 gcgattcgtc ctccctcctc gtggtccacg tcatcgttaa tcgagtggcc acgggacgca
     2701 aggaacatgt gggttaccgc atgtggccat gcaaaccccg gcggccggaa tcggccttcc
     2761 gtgaacgggc cgccggaaaa cggggaggcg acgtgccgcc ggtgaaagcg gcggcccctc
     2821 cgccgtccgg tcagggacgg aggaggggcc ggttccggcg ggcggacggg cggtcagcgg
     2881 cggccgggca gcagcaggat cccgtccccg acgctgcgct cgcccgtgcc gtcggacggg
     2941 cggttgacga gcgcgccgcc cgccgtcgcc atcgtggtgg atccgccgcc gtcgaagttc
     3001 atggcctgca cggcgccgag cgaccgcatg accttcgcgg cctcctcgat ggacaggccc
     3061 tcgctgaagc ccggcgcgcg gccgtccgcc gcgaccatca gcagccgccc gcgccggtcg
     3121 acgccgacca tggtgcgcgg gttacggcgc agcacccagt tgtaatagaa cgactggtcg
     3181 tcgccggtgt ggacgaggcc gtcggcgtag gcgtcgaccc aggcgcggcc gtcccggacg
     3241 agctgcgggc ccgcgccgag gatcgacgtc cgggcgtcga ggcggacggg gcgcccgcgg
     3301 tcgagcacct gctcggagat cgcgagcctc gtgcccgggc gggcgtggtc gcgcagccag
     3361 tccgcggccg tcccgatcgc ctggacggtg cggccgccgg cggggatggc ggtgccgcgc
     3421 gccggccgca gctccgtcac gcgcccggac gcgtccagca cggcctcggc gccgtcgccg
     3481 gccgggctcg acgcgccgta gaccggggtg aaggcgacca gctcgtcggg atcggtgcag
     3541 gtcacgtcgt gctgcgggcg ctcggtcggc tggtcgccgc ccacgccgcc gcagttgtcg
     3601 accagtcccg gcagccggtt gacgccgtcg agctcgcggc cggcccggtc ctgcgcccgg
     3661 acgcgcagca cggtgctgag ctgcgcgaac cgcgcgccgc ggccgctctg cggcaggatc
     3721 agcgcggtgc gcccgttgtt cgcctcgctg gtcagctcgc cgttcagcac gtgcaggccc
     3781 gcggagccgc ccttgccgcc ggcggtgaag taggtgccgt tgacgccggc gacggcgccg
     3841 ctggtgcgca ccaggtcgga caccttgtcc gtggcggtca gcgtgccgcc gacggaggag
     3901 accacgcgtc cgccgaaccg gcccgggtcg acgctgatca cgtgcaggcg ccacggtccg
     3961 gtcgccgccg tcccgtcctg cgccgtgtag gaggtgcccg ccgagaaccc ggcggcgttg
     4021 agggccggga ggtccttctt ggcgtcggcc tcggtcgcgt accggccgac ccggacgacg
     4081 tagccgaccc ggcccgcgcg cacgtcggcg aagcgcgggc cgtccacggc ttcgacgcgc
     4141 gggtggaatc cggcggactc cagccggcgc gcggcgtcct cggcggtcgc cttcggcgcg
     4201 atcacggcgg aggtgttgcc gggcacggcc agccggacgt gcagcgtcca gacgtcggcg
     4261 gcggacttct cgccccggac gatcgtggtg agggtgacgc ccggagcgag ttggcgcgtg
     4321 tcgcgctgct cgtgcaggga ggaggggccg agggggaggg cggaggtgcg ggcggcggcc
     4381 gtggccggag cggccagcgc ggtcacggac gccgcgcaga gcgccgccgc gaggaggggg
     4441 cgtgtcatgc cgtcatgatc gacgcggccg gtgacgtggg agtggacgcg agatgatcat
     4501 tggcgcgccc taggatctca aaccccagca ccgaccaccg agggagcgca tgtgtcggcc
     4561 gagccgctgc acgccgtgag tttccgcctg tcctacggcg actgcgatcc cgcggggatc
     4621 gtctactacg ccaactacca gcggtggatg gagcggacgc acaccgaatg gtggtacctg
     4681 cggggcctgc ggttcgacga gctgcccgcg cggctcggcg tcgcggtggt gacgcgcgcc
     4741 tccaccgccg agtacgagcg gaccatcacc ctgttcgacg tcgtggagtg ccggctgttc
     4801 gccgagcggc tcgggcgctc ctcgttcgcg gtgcgcggcg acttcgtcac cgaggacggc
     4861 gaccgcgcct gcaccaccgc cctcaccctc gtctgcgtgg acgccgccga cccgcgccgg
     4921 acggtgccgg tgcccgagcc gatgcgcctg gccctgggcg ccgggaacgc cgcgctcagc
     4981 ggctgaaccg gcggcgcggg ctacgcggtc agcgggacgc tggactcgga ggtgacggcc
     5041 aggaaggtga gcgtctcctg gaggtacagc cggacgtgct cggcgtcgtg cgacaggtac
     5101 ccgatcgaca ggtcctgccc gaggtgcagc tcgtagtcgc cgccgcgggt ggacagcagc
     5161 agcgcaccgt cgagcgccgg cgcccagatg atctcgccgt cgcccagcag ccgcgcgagg
     5221 tggtggcgga cggggtagcc gtggtcggtg gtctccgaga cggccgtgta ggcgtccgcc
     5281 gacagcagca ggctgtacgg cccgccgacg ccggccagcc gcagcgcgct cagcgccttc
     5341 gcgaccgcgt cggggacctc ccgcgcgtcg gccggcagcg gcagcgccgg gttggagctg
     5401 ccgggcgcga tgccggtgat cccggccgct tcggagccgt ggaagacggc gtggtcctcg
     5461 gcgaacgcga tcttcttcgc ggcgtccttc gcgggctgcc agtccgggtc gagcgcgccg
     5521 cgctcgacgt cgtccacgtc ctgccgggcg accgtgaaag ggacccgcag ctcgatgatc
     5581 ggctgggccc gccgccggtg ggccagcacc ccctcggccg gcggggcgag cgggtcgacg
     5641 tgcccggtgc cgacggcgga cagcgccggg cccgcggggc cgacgaggtc caccacgcgg
     5701 cggcccgcga ggtggcgggt gaaggtgcgg cgcacctcgg cctcgaggtc ggtccaggcc
     5761 gcgtccgaga tcggggcgag ttcgcggtgc aggttgttca tggtgtgccg ttccttctca
     5821 ggctgccgat gccgagtgag ccgtccgcgg ccgggccgcc gcccggcggc ggttgcgggt
     5881 ccggctccgc ggcggccgtg gcggacgcga gggcgaggcg gggcgacggg tcgtcgagga
     5941 agtcggccgt gggcacgaag aacaggccgc cggtgaccgc cgtcgagaag tccaggatgc
     6001 ggtcgtgatt gcccggcggg tccccgatga acatgttctc gagcatcctc tcgatgacgt
     6061 cgacgcggcc gacgtagccg atgaagtagg tgccgaactc gtcggcgccg atcgacccga
     6121 acggcatgtt gtcccgcacg atctggtgct gggtcccgtc cgggtcgacg atcgtgttga
     6181 gcgcgacgtg cgagttcggc ggcttcgccg cgtcgggcag ctcgatgtcg ctgagcttcc
     6241 tgcggcccat cgccagctcc tgcgcctcga ccggcagcgc gttccagccg cgcatgtcgt
     6301 ggaggtactt ctggacgacg acgtagctcc cgccggcgaa ggcggggtcg tcgtcgccga
     6361 tggtgacggc gacgacgccg tccgggcccg acgggctctc ggtgccgtcg acgaagccga
     6421 gcaggtcccg ctcgtcgaag taccggaagc cgtgcacctc gtcggcgacg tcggcgtgcc
     6481 cggcgagccg ggtcatgagc tgccgcgcca gctcgaagca caggtcgggg cggcgggccc
     6541 gcaggtggaa cagcaggtcg ccgggcgtcg acaccgcggt gtgcacggcg cccgcgagcg
     6601 tccggaacgg gtgcaggccg gccgggcgcg gcgggccgta gagccggtcc cacaggtgcg
     6661 cgccgatgcc ggtcacgcag ctgaggccgt cctcggggga gcggaagccc acggcgcgcg
     6721 tcagcccggc gatgtcgggc agcagcccgc gcaccgtgtc ctcaccgccc gggcggaccg
     6781 tcacgaccag gaagatggcg gccgtcgcgg gcggcgccag cacgtcctgc ggctgtccct
     6841 gctgcggcat cgcgtccccc cgccgtcccc gccccgggtg gcgcgcaccc gatcatcgtc
     6901 agcgccggtc tacccccggc cccgaccggg tgaaaccgga ccggggcagg cacgggggca
     6961 gttttgtccg tgacgacagc cgcccttggt gcggccggcc cggacggcgc gccaggtgag
     7021 ggccgcgggt ccgtgggtat cctcccccgg tcgatcagcg ccggcgggac gagacgcggg
     7081 agggcacggg ggcatggccg agcagttctc cgtcggggac cacgtcaggt ggaactccga
     7141 ggccgggtac gtcgagggcg tcatcatcag gaagcacacg cacgacgtgg agtacaaggg
     7201 ccacatgcgc cggtgcagcg aggacgaccc ccagtacgag atcaagagcg acagaaccga
     7261 ccacgtggcc atgcacaagg gcccggccct gaccaagctg taggcgctcc gggagggacg
     7321 cgccgatgcc ggtgccgccg ccgtccgtac ccgcccagcg gtggatctcc gtcgccgccg
     7381 gggccgtggt ctgcgcgctg gtgtgcgtcc tggcgaacgt cccggtgggg ctgctgtcgg
     7441 ggatcgcggc ggcggccgcg gtgttcgtcc tgacgggggt ggcggcggtc tggccgatga
     7501 ccgccgggag cacccggcgc catgcgcgcc gcgagggctt ccggccgctc gtcgccgagt
     7561 cggtcgtcgt gacggccgcg atcggcagcc tggtcggggt cgcggtgctg ctggccgtcg
     7621 ggagttccgg cacccggagc gcggccgcgg cggtcggcct gttcagcgtc ttcatgagct
     7681 gggggatgct gcacctgatg tacgccgtcc gctacgcgca cctctactac ggcgagcccg
     7741 ccggcggaat cgacttcaac agcgaggagt cgccgtcgta ccgggacttc ctctacttca
     7801 gctacaacct cgggatgacg taccaggtgt cggacaccga cgtgtcgagc acggcgatcc
     7861 gagccgtggt gctgcgccac acgctgctgt cgtacctcta cgggacggtc atcctcgccg
     7921 cgaccatcaa cctcgtcgcg ggcatcatct cgagctgaca ccgccgcgtc acggcgcgac
     7981 cagcagggtc tgcggggcgg cggccttcag ccgcatccgc agccaggcga gctgcgcgcc
     8041 gatccgcggc tcggacgccg cggcgacgcc gatcagctca cggtcgcgcg cgccggccgc
     8101 cgcctgcccg accagcgtcc aggtgatctc gaccaggccg gcgagcccgt agaggtcctg
     8161 gaggtcgcgc agcagcccga cgggaccggg ccggacggcc gtgagcccgg gcgggtgcag
     8221 ccggtccggc tcggcctgcc ggcggtcggc gtaccgctcg gcgacggggt cgagcgcgtc
     8281 ggcgtgccgg gtgcactggg cggcgaagcc ggtggaggtg tggccgcaac tgggacaacc
     8341 ccgagcacgt gcgcgaggcc tggaagcgct gcaccgcggg ccgtccggtg gactacaccg
     8401 ggctcaccta ccggatgctg cacgaccgtc gacaccagcc gctcgcccgt gccgaagatc
     8461 tccgcggcgc ggacgatgtc ggcggccggc acgccgcatc agcgcgaggt cggtgccgtt
     8521 gcggatcggc aggtgcaccg tcgcgcgctc ggcgaccttg gtgcggcgcg ggtcgatgca
     8581 caccagccgc ggcgggttcg ggccgtccag ccggtccagc acgcgggccc acggcacccg
     8641 tctgcgtctc ggcgacgttg tgcccgaaca ggaagacggt gtcgcagtgg tcgatgtcgg
     8701 tgtaggagcc gggccgcccg tcgcagccga cgctcacccc gaacgccgtc gccgcggtgg
     8761 ccgtgcacag ccgggtgttg ccgtccatgt gcggggtgcc gatcccgccg gcgaggaacc
     8821 cgtccacccg gtcgggccag ggggcgccgg atccgtgggg cgtgcgtgca cccagatgtc
     8881 ggccacgcgg tcagcgtcca tcgtccgcct tcctttcctc ggaccgcggc gggccgccgc
     8941 ggtccgctgg tcctagcgcc ccgtccggct gcgccggggg cggttggagc agcgccgcgc
     9001 ccatggcgac ggccaccgac agccaggccg cgaacccggc ccacacctcg acccgcgcga
     9061 tgtcgaccat gaaccccagg ccccgcgccc ggccgtaact cgcggtcgcg gccgcgtaca
     9121 tgccgagggg gaataccatg ctccacagcg tgggctcgta gctcaccggc gcgccgcgca
     9181 ccaggtgccg ccacaccgcg aagaacagca gcagcgggat ccaccaggtg ccgaaggccc
     9241 acagcaggaa cgcgagcccg gacaccacct gcccggtcga cgccagcacc ggcagcccgg
     9301 ccggcagcgc caggatcttc gccgcggcga ggacggtgat ggcggtcgcg cccatgtaga
     9361 cccagtacgt cgggctcagc gcgtgcggcg tgacgggctc gtccagcagc cgggcggtga
     9421 ccagcccggc cagcatcagg tacagcacga ccccgatgcc ccacaacgcc accgccagcg
     9481 gcgcgagcgc ccccgcccac ccgtgcctcg ccccggccac cgtggccgcg gagacggcga
     9541 gcgactgggt ccccaccacc cacatgaacc agctcccgtt gatcccgggc aggacggggt
     9601 cgtcgcggtg cccgacgatc aggacgccgg ggatgccgta ggtgagcacc agccagatcg
     9661 ggacgctgac cagcgccagt accgtgctcg tcagcacatg gtggtcgagc gccagccgca
     9721 cgcccaccac gttcggcgcg gccaccagcg agaagtagcc gaaccccttg ctcgggttcc
     9781 gggcgtcggc gagcaggcgc cgcgggtagg cgaccgcccg ccacacgtac gcggccgcca
     9841 acacgaccag tgcggccgcc gcgacgccga gcagcgcgtc cgacagcgcg tcccagccga
     9901 acagctccag gccggtcgag acgatcccgg tcgccatcac cagcgcggcg tacccggggt
     9961 gcaggccggc gacctggcgt tggacgcggt tcatcgcggg cggctcaccg gtgccggccc
    10021 gcctccgggc cgcccgcggt cccgcccatc aggtctcgaa ccggacacag gtcccgtagg
    10081 cgacgtactg ggtctcggtg tggatgcgca tgacgtcggt cgtggtgaag tcggtgcggc
    10141 ccagcacgtt cgggacggcg aggaggcaca ccccgaggag cgcgtcgggc cgcaggtcgg
    10201 cgctcgcgcg ttcgaggttg gtgaaggcgg actcggggct cgagccgggc tggccggccc
    10261 tgatccccag cgtggtgacc ccctcctggt cgtcggggat gcggtccgtc gtgtacatcg
    10321 gtgtgctcac cggggagtga ttcccccgtg gagccgatgc cctctcagcg gccattcatg
    10381 gctcgggaaa cttcgtgccc cgcggacacg ccgggattcc gggcggtatc cgcgcgcccg
    10441 cccgccggtg atctgccgaa gcctggttcc ggtttatggt ttcttggggc gcttcaattg
    10501 cgccatatgc tctgttctgt cgcatatctg ggtacggtcg cgggatggtg gagctggatc
    10561 tggccacggt gttcgacgcg gagccggtcg cgtgcgcggt gatctcgccc gacctggagt
    10621 tcgtcgcggt gaaccgggcc tacgaacggc tggccgggcc gcgggagcgg ctcatcggcc
    10681 gccgcgtgtt cgacgtgctc cccggcggcg cggacggccg gaacgccgcc cactggcgga
    10741 cggccctgga gtgggtcctg gccgaaggtc agccctacgt catgccgtgg gggcgctatg
    10801 a
//
