LOCUS       NZ_CP053892            10795 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Actinomadura verrucosospora strain NRRL:B18236 chromosome.
ACCESSION   NZ_CP053892
VERSION     NZ_CP053892.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M.
  TITLE     Actinomadura verrucosospora NRRL-B18236 (PFL_A860) Genome sequencing
            and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2016
            Assembly Method                   :: Newbler v. 3.0
            Assembly Name                     :: A860_AS2.mpe1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013211575.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,277
            CDSs (total)                      :: 9,181
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 96
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 18 of 71
            Pseudo Genes (incomplete)         :: 57 of 71
            Pseudo Genes (internal stop)      :: 2 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:15:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9378362
            Orig. end                         :: 9389157
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Peter Leadlay,
            NRRL-B18236 (patent collection, not in online catalogue).
FEATURES             Location/Qualifiers
     region          1..10795
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="30"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10795
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10795
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5795](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5795)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(761..773)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            761..1702
                     /gene="bla"
                     /locus_tag="ACTIVE_RS42240"
                     /old_locus_tag="ACTIVE_8474"
     CDS             761..1702
                     /EC_number="3.5.2.6"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="bla"
                     /gene_functions="resistance (resist) ClassA"
                     /gene_kind="resistance"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589284.1"
                     /locus_tag="ACTIVE_RS42240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8474"
                     /product="class A beta-lactamase"
                     /protein_id="WP_246342526.1"
                     /transl_table=11
                     /translation="MPNAIFERSALSRRALLAGAVLLPLAGCDSGHRSGSRPTPKPARS
                     TSASPDTSKFAALEREHRARLGVYAVATGTGATVAYRADERFAFCSTFKALAAAAVLHG
                     NPLGHLDKRVTYTRADVDSISPITQDHIASGMTIRQLCAAAIRFSDGTAGNLLMRDVGG
                     PARLTAYLRGLGDTVSRMDDYEPALNKVGPKDPRDTTTPRAIAADYRRLVLGDALPAAQ
                     RALLKDWMLRNTTGAARIRAGLPKGWTVADKTGNGNWGRVNDVAIVWPPGTAPLVMAVM
                     TDRPGFGARQDDALIARAAARVVTELGVTGRP"
     misc_feature    1778..1792
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.5"
                     /tool="antismash"
     misc_feature    complement(1789..1803)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.82"
                     /tool="antismash"
     gene            1858..3192
                     /locus_tag="ACTIVE_RS42245"
                     /old_locus_tag="ACTIVE_8475"
     CDS             1858..3192
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /locus_tag="ACTIVE_RS42245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8475"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_173100205.1"
                     /transl_table=11
                     /translation="MRAPGHATEPGCGTEPGRSGRDAPDELDAPDGDVRAALAGALREA
                     VPAIVAETVEEVRRDVAEYAAPACRDALELAAGCTLGAFAGLVARPGPPPADLLGLFQR
                     IGAAEVQEGRPASALQNAANVATRVVVRHLSRVADALDAEVPPGLYGQVVSALFPLLNA
                     LTVAVAQGRAEAARDPAVLLLRRRRALLDALLARPATEPRRIAGLAREAGWPLPRRAAA
                     VALAPGTGPSRSRVLPPQVLDGRHLAEPCLIVPDPRDPAHRRALEPLLADLASAVGPAV
                     EPAALGRSLAWARQALALALDGTLGGVLDGEPPGDPPGRGPVIAADHAPTLVVLRHRDL
                     VEHSARRRLNPLLKIRPNRRTQYARTLQAVLECGFNASAAAVRLRVHPQTVRLRMRKLD
                     ELFGAEIHDPSLHVELLMALRLWLATTRPGGQPDWFRNGPASAPA"
     gene            3311..3784
                     /locus_tag="ACTIVE_RS42250"
                     /old_locus_tag="ACTIVE_8476"
     CDS             3311..3784
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629751.1"
                     /locus_tag="ACTIVE_RS42250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8476"
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="WP_173100206.1"
                     /transl_table=11
                     /translation="MSSTVPDLGDTEQLKTLLEDVASPDQLRELLDAPGVDDAMIDGFV
                     AAVGAEGVLDKVFGLMGEHFVAERAGNDAGVIRWDVTAPGGTLSYGLSIRDGAAIGERG
                     APDGPKVTLTLSVPTLLRLCAGRLNGVQGFMTGQIKLSGDMMFGAKLPGWFDY"
     gene            complement(3925..4818)
                     /locus_tag="ACTIVE_RS42255"
                     /old_locus_tag="ACTIVE_8477"
     CDS             complement(3925..4818)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629752.1"
                     /locus_tag="ACTIVE_RS42255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8477"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_216858140.1"
                     /transl_table=11
                     /translation="MADFRIDDLARAAGTTVRHVRGFQERGLLPPPRMKGRVGYYDDTH
                     LARIHLITRLKDRGYTLASIGEMLSAWERGHNLADLLGLERVIHDPWSDEEPARMTLAQ
                     VVRRFYPQVPEEALEGRTPADFPRLRRAEQLEFIRWTGEGYEVPSPRLLEVGAGLVEAG
                     IELDAVFEIADRLRGDVDAIARQFVRLTVEHAGLDARLADRDIREVGEVIQRLRPIGMQ
                     AVQGLLARSLHAEIQAEFIRQIETIGPPPPKPGSAARPAEPPAHAGATPPPVAAQPPAQ
                     VTADATSLPSADGRPR"
     gene            complement(5001..5795)
                     /locus_tag="ACTIVE_RS42260"
                     /old_locus_tag="ACTIVE_8478"
     CDS             complement(5001..5795)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980848.1"
                     /locus_tag="ACTIVE_RS42260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8478"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_173100207.1"
                     /sec_met_domain="Linocin_M18 (E-value: 6.2e-97, bitscore:
                     322.7, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISAAAWDDLEAEIRRTVTRNLAGRRLVDVPEPGGV
                     ALAAVTTGHLDPIDAPADGVQAHVRRARPLVELRVPFTVARQDVDDVERGAKDPDWQPA
                     KDAARLIARAEDRAIFEGYAAAGIEGLRAASANPAIALPDEPREYPNAVAQAVTALRLA
                     GVDGPYSLALSADAYTAVNETSDHGYPIHEHLARLVSGDIVWAPAISGAFLLSTRGGDF
                     ELRVGQDLSVGYESHDATSVRLYLQETFTFLTYTAEAVVALG"
     gene            complement(5792..6853)
                     /locus_tag="ACTIVE_RS42265"
                     /old_locus_tag="ACTIVE_8479"
     CDS             complement(5792..6853)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540968.1"
                     /locus_tag="ACTIVE_RS42265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8479"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_246342527.1"
                     /transl_table=11
                     /translation="MPHTAISSGDGHDTPDPARAGPPVSAPRAGASQEVLGPLTGSAIF
                     LVVTVDPGGEPVVAELLPDLAGLVRAVGFRVPGGGLTCVTGIGSDAWDRLFSGPRPAGL
                     HPFTELNGGVHTAPATPGDLLFHIRAHQMDLCFELAAQIMDRLAGAVTVVDEVHGFRYF
                     EQRDLLGFVDGTENPTGEDARAAVVIGDEDAEFAGGSYVIVQKYLHDMRAWNALTVEQQ
                     EDAVGRRKLSDIEIPDAAKAPGAHVALTSITGPDGEEREILRDNMPFGSLRDGEFGTYF
                     IGYSATPDVTEEMLRNMFLGDPPGVHDRLLDFSTAVTGTLFHVPTADFLDDPPPLPSDD
                     GSLGIGDLKRSAP"
     gene            6972..8189
                     /locus_tag="ACTIVE_RS42270"
                     /old_locus_tag="ACTIVE_8480"
     CDS             6972..8189
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634798.1"
                     /locus_tag="ACTIVE_RS42270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8480"
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_173100209.1"
                     /sec_met_domain="ADH_N (E-value: 8.2e-29, bitscore: 98.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 3.3e-11, bitscore:
                     42.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVVWHDVGEISLDEVPDPAVRDPEDAVVRITASAICGTDLHFV
                     RGTMPDMEPGTVLGHEAVGVVEEVGRDVRNFVPGDRVVIPSTIGCGRCSYCRAGYYAQC
                     DVANPNGPSAGSSFYGGPRSTGPFDGLQAEYARVPYAHTNLVAIPPGVTDEQALMVSDI
                     LPTGWFGARLAEVGPGDTVAVLGAGPVGQLAVLSARIQGAGRVFVVDGHADRLETARLQ
                     NAETVDFNTEDPVAVVREMTGGIGADRVIDAVGVDAESPASGPAAEAITKEDRREFERE
                     QAQAAPRTGQDGVRWRPGDAPGEALRWAVDMVAKAGTIGIVGVYPPHFTSFPLGLAMNR
                     NLTVKAGNCNHRRYVPGLLSRIAKGGADPTTILTQQARLPGVIEAYEAFDRREPGWTKV
                     ALEVAG"
     gene            8219..8554
                     /locus_tag="ACTIVE_RS42275"
                     /old_locus_tag="ACTIVE_8481"
     CDS             8219..8554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852354.1"
                     /locus_tag="ACTIVE_RS42275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8481"
                     /product="DUF2795 domain-containing protein"
                     /protein_id="WP_173100210.1"
                     /transl_table=11
                     /translation="MKIADTRRVEELLGDLAFPASKERIVEHAHAERPLSEEERALSSL
                     PLGTYGSAAEVLRSLPIDPDPGRTPAEQAYQRRHHNKQGLAEHMRDTERPPVQDELRGD
                     DTPHWHP"
     gene            8728..10413
                     /gene="ctaD"
                     /locus_tag="ACTIVE_RS42280"
                     /old_locus_tag="ACTIVE_8482"
     CDS             8728..10413
                     /EC_number="7.1.1.9"
                     /GO_function="GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ctaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634758.1"
                     /locus_tag="ACTIVE_RS42280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8482"
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="WP_173100211.1"
                     /transl_table=11
                     /translation="MTAVQGAPDTSTADALARHRKGTRVGDILATTDHKMVGYLYLGTS
                     FAMFLIAGLMAMLMRAELMQPGMQVMNRLQYNQLFTIHGTIMLLLFATPLFAGFANVIV
                     PLQIGAPDVAFPRLNTLSYYLFLFGAVMVLAGFLMPGGAAAFGWFSYSPLSSSTYSPGL
                     GADMWVMGLIVSGFGTIFTSVNLMTTIIAMRAPGMVMFRLPIFTWNALLTSVMVLIAFP
                     VLTAALFALEADRKFGAHIFDGATGGALLWQHLFWFFGHPEVYIVALPFFGIVTEIIPV
                     FARKPVFGYLGMVCATIAITGLSMTVWAHHMFATGQVLLPFFSMTSFMIAVPTGIKFFN
                     WVGTLWKGHLTFETPMLWAIGFLVTFLFGGLTGVILASPPMDFHLTDSYFVVAHFHYVL
                     FGTVVFAMFGGFYFWWPKMTGKKLDETWGKIHFWSLFVGFHTTFLVQHWLGAEGMPRRI
                     ADYSKQFATLNLVSSIGAFILGASTFAFLWNIYRTHRRARPVETDDPWGHGASLEWATS
                     CPPPRHNFETMPRIRSFRPAFDLHYPPKEPPQESEPALPQPPTT"
     misc_feature    complement(10566..10582)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.67"
                     /tool="antismash"
ORIGIN
        1 ctacggcgcg ggcgacgaac cggagaccgc gctgtcgctc ccgctcggcg cgggccggct
       61 cacccggcac cacctgcccg gccggccgcc cgtccgcaag cgcacccgca aggcgctgcg
      121 cgcgcacgtc cggtccgtgc tggacgaggc gaccaccggg ctcgccgagc ggcccgcgcc
      181 ggtccgggcg gtcgcgacgt ccaagacgtt cacgcagctc gcccggctgt gcggcgcgcc
      241 gaaggcgaag gccgggccgt acgcgccgcg cactctcgcc cgcccggacc tgcacggctg
      301 gatcccgaag ctggcgaagc tgccggacga ggagcgcgcg aggctgcgcg gggtgaagcg
      361 gtcgcgggcg caccagatcc tcgccggggc catcgtcgcc gaggcggtca tggacgtcct
      421 cgggctcgac cggctcgacg tgtgcccgtg ggcgctgcgc gaggggatct tcctgcaccg
      481 gctggacgcg ctggccgccg ggcgcccggc ggggccgccg ttcgggaccg tcggcgacgg
      541 ggcgggcgcg ccgtacccgt gccccgtccc ggtcaggccg ccgcagcctg gcgcggggcg
      601 ggcggcggcg gcgcggctgc acgcggtcgg ctccggctga ccggcggcgc ccgcaggacg
      661 ggtgcccgta cttcacctgc gaagatacgg gttcgcctat cggtcgctta tgcacactgg
      721 acttggacat cgcccggggc gtcgtgctgt catgacggcc gtgccaaatg cgatcttcga
      781 gcggtccgcc ctgtctcgcc gcgccctgct ggccggggcg gttctcctgc cgctggcggg
      841 ctgcgactca gggcaccgct cgggctcgcg tccgaccccg aagccggcgc gcagcaccag
      901 cgcctccccg gacacgtcga agttcgccgc gctggagcgc gagcaccgcg cccgtctggg
      961 cgtctacgcc gtggcgaccg gtaccggggc cacggtggcc taccgggcgg acgagcggtt
     1021 cgcgttctgc tccacgttca aggcgctcgc cgcggcggcg gtgctgcacg gcaacccgct
     1081 cggccatctc gacaagcgcg tcacctacac ccgggccgac gtcgactcga tctcgcccat
     1141 cacccaggac cacatcgcgt ccgggatgac catcaggcag ctgtgcgccg ccgcgatccg
     1201 gttcagcgac ggcaccgccg gcaacctcct gatgcgcgac gtcggcggcc ccgcccgcct
     1261 caccgcctac ctgcgcggcc tgggcgacac cgtcagccgc atggacgact acgagcccgc
     1321 gctcaacaag gtcggcccga aggacccccg cgacaccacg acgccccgcg ccatcgccgc
     1381 cgactaccgc cggctcgtcc tcggggacgc gctgcccgcc gcccaacgcg ccctcctcaa
     1441 ggactggatg ctgcgcaaca cgaccggcgc cgcgcgcatc cgggcggggc tgccgaaggg
     1501 atggacggtg gcggacaaga ccggcaacgg caactggggg cgggtcaacg acgtcgccat
     1561 cgtgtggccg cccggcaccg ccccgctggt catggcggtc atgaccgacc ggccgggctt
     1621 cggcgcgcgc caggacgacg cgctgatcgc ccgggcggcg gcccgcgtcg tcaccgaact
     1681 cggtgtgacc gggcggcctt gacgaccatc ccgtaatctt gcgggacata cttctgtggt
     1741 ccgtgtgtct tgacattcct tccatgagcc gtccacgctt gttccgttaa tttgcggtac
     1801 atagatcgac gatacggtgc ctcgcggggc gatggccgtc gggaggggac gcgcgcgatg
     1861 agggctcccg gccacgccac cgaaccgggc tgcggcaccg aacccgggcg gtccggccgc
     1921 gacgcgcccg atgagctcga tgcgcccgac ggcgatgtgc gggcggccct cgcgggcgcg
     1981 ctgcgcgagg ccgtccccgc gatcgtggcg gagacggtcg aggaggtgcg gcgcgacgtc
     2041 gccgagtacg ccgcccccgc ctgccgggac gcgctcgaac tcgccgccgg atgcacgctc
     2101 ggcgcgttcg ccggcctcgt cgcccggccc ggcccgccgc cggccgacct cctcggcctc
     2161 ttccaacgca tcggcgccgc cgaggtgcag gagggccgtc ccgcgagcgc cctgcagaac
     2221 gccgcgaacg tcgcgacccg cgtcgtcgtc cggcacctgt cccgggtcgc cgacgcgctg
     2281 gacgccgagg tgccgcccgg cctgtacggg caggtcgtct ctgcgctctt cccgctcctg
     2341 aacgcgctga cggtggccgt cgcgcagggc cgcgccgagg ccgcccgcga ccccgccgta
     2401 ctgctgctgc gccgccgccg cgcgctgctg gacgcgctgc tcgcccgccc cgcgaccgag
     2461 ccccggcgga tcgccggcct ggcgcgggag gcgggctggc cgctgccgcg ccgcgccgcc
     2521 gccgtcgcgc tggcccccgg gaccggcccg tcccgttccc gcgtgctccc gccgcaggtg
     2581 ctcgacggcc ggcacctcgc cgagccgtgc ctgatcgtcc ccgacccgcg cgacccggcg
     2641 caccggcggg cgctcgaacc gctcctcgcc gacctggcct cggccgtcgg ccccgccgtc
     2701 gaaccggccg cgctcggccg ctcgctggcc tgggcgcggc aggcgctcgc actggccctg
     2761 gacggcacgc tcggcggcgt cctcgacggc gagccgcccg gcgacccgcc cggccggggc
     2821 ccggtcatcg ccgccgacca cgcgccgacc ctcgtggtgc tgcgccaccg cgacctcgtc
     2881 gagcattccg cacgccgtcg gctgaacccg ctgctgaaaa tccggccgaa tcgccgcacg
     2941 cagtatgcgc gcactctcca ggcggtgctg gaatgcggat tcaacgcgtc cgcggcggcg
     3001 gtccggctgc gcgtccatcc gcagaccgtc cggctccgga tgcgcaaact cgacgaattg
     3061 ttcggcgccg agatccacga cccgtccctg cacgtcgagc tgctgatggc gctgcgtctc
     3121 tggctcgcga cgacccgtcc cggcgggcag cccgactggt ttcggaacgg ccccgcaagc
     3181 gcacccgcat aaacctttga acaggtgagt gccgcccatc gcgatttcgt catcggtgcc
     3241 gttatccggc ggccgcgatc ctggaatcct cggaccggag aacgcgccgc aacgaccgga
     3301 gggaattccg atgagcagca ccgtgcccga cctcggggac accgagcagc tgaagacatt
     3361 gctggaggac gtcgcctcgc ccgaccagct ccgcgaactg ctggacgcgc ccggcgtgga
     3421 cgacgcgatg atcgacgggt tcgtcgccgc cgtcggcgcg gagggcgtcc tggacaaggt
     3481 gttcggcctg atgggcgagc acttcgtcgc cgagcgcgcc ggcaacgacg ccggcgtcat
     3541 ccgctgggac gtcaccgcgc cgggcggcac gctgtcctac ggcctgtcga tccgcgacgg
     3601 cgccgccatc ggcgagcgcg gcgcgccgga cggcccgaag gtgacgctca cgctgtcggt
     3661 gccgacgctg ctgcggctgt gcgcgggccg gctgaacggc gtgcagggct tcatgaccgg
     3721 ccagatcaag ctgtccggag acatgatgtt cggcgccaag ctgccgggct ggttcgacta
     3781 ctgatcggtc cctgccgccg atccggcccc gtcccggtct ccgggtgcgg ggccgcacgc
     3841 gcgggagggg caggtcagac gggcggctga gaaaggcggg tcagccgggc aggtcgggcg
     3901 cggccttctc gcccggcccc gcggtcacct gggacgaccg tccgcggacg gcagggacgt
     3961 cgcatccgcg gtgacctgtg cgggcggctg cgcggcgacc ggcggcggcg tcgcgccggc
     4021 gtgcgcgggc ggttcggcgg ggcgcgcggc gctgccgggc ttcgggggag gggggccgat
     4081 ggtctcgatc tgccggatga actcggcctg gatctcggcg tgcagcgacc gggccagcag
     4141 cccctgcacc gcctgcatgc cgatcggccg cagccgctgg atcacctcgc cgacctcgcg
     4201 gatgtcccgg tcggcgaggc gcgcgtcgag cccggcgtgc tcgaccgtca gccggacgaa
     4261 ctggcgggcg atggcgtcca catcgccgcg cagccggtcg gcgatctcga agacggcgtc
     4321 cagctcgatg ccggcctcga cgagccccgc gccgacctcg agcagccgcg ggctcggcac
     4381 ctcgtagccc tcgcccgtcc agcggatgaa ctccagctgc tcggcgcggc ggagccgggg
     4441 gaagtcggcg ggcgtgcggc cctcgagcgc ctcttccggc acctgcgggt agaaccgccg
     4501 tacgacctgc gcgagcgtca tccgggccgg ctcctcgtcc gaccacgggt cgtggatgac
     4561 ccgttccagg ccgagcaggt cggccaggtt gtggccgcgc tcccacgccg acagcatctc
     4621 gccgatgctg gccagcgtgt agccgcggtc cttgagccgg gtgatgaggt ggatgcgcgc
     4681 cagatgggtg tcgtcgtagt agccgaccct gcccttcatc cggggcggcg ggagcaggcc
     4741 gcgctcctgg aagccgcgga cgtgccggac ggtggtgccg gccgcgcgcg ccaggtcgtc
     4801 gattcggaag tcagccatgc ccgtctcttc gagtcggcgg tcgatctcct catcgtactc
     4861 acaagcgctg gcgcggttat ccgcggtcca ttgagtcatg ggccgcgaat tcacggcaca
     4921 ttgtgcggcg gcgcggcccg cgcccgccgg gcgtcggccc agcgcgtctc ctgccgtcag
     4981 cccggcgcgt ccccctgccg tcagcccagt gcgaccacgg cctccgcggt gtaggtgagg
     5041 aacgtgaacg tctcctgcag gtacagccgg acgctcgtcg cgtcgtgcga ctcgtagccg
     5101 accgacaggt cctggccgac gcgcagctcg aagtcgccgc cccgcgtgga cagcaggaac
     5161 gcgccggaga tcgcgggcgc ccagacgatg tcgccgctga ccagccgggc gaggtgctcg
     5221 tggatcgggt agccgtggtc ggaggtctcg ttcaccgccg tgtaggcgtc cgcgctgagc
     5281 gccagcgagt acgggccgtc caccccggcc agccgcagcg ccgtgacggc ctgcgcgacc
     5341 gcgttcgggt actcgcgcgg ctcgtccggc agcgcgatcg ccgggttggc ggacgcggcg
     5401 cgcagcccct cgatgcccgc cgcggcgtac ccctcgaaga tcgcgcggtc ctcggcgcgg
     5461 gcgatcagcc gggcggcgtc cttggcgggc tgccagtccg ggtccttcgc gccgcgctcg
     5521 acgtcgtcga cgtcctgccg ggccacggtg aacggcaccc gcagctccac cagcggacgg
     5581 gcgcgccgca cgtgcgcctg cacaccgtcg gcgggcgcgt cgatcgggtc gaggtgcccg
     5641 gtggtgacgg ccgcgagcgc cacgccgccg ggctccggca cgtccacgag ccgccgcccg
     5701 gcgaggttgc gggtgacggt gcggcggatc tccgcctcca ggtcgtccca cgcggcggcg
     5761 gagatcggtg ccagttcgcg gtgcaggttg ttcaaggtgc gctcctcttc aggtcgccga
     5821 tgcccaggga tccgtcgtcg ctgggaagtg ggggcgggtc gtcgagaaag tcggccgtgg
     5881 ggacgtggaa cagggtcccg gtcacggcgg tcgagaagtc gagcagccgg tcgtgcacgc
     5941 cgggcgggtc gccgaggaac atgttccgca gcatctcctc ggtgacgtcc ggggtggcgg
     6001 agtagccgat gaagtaggtg ccgaactcgc cgtcccgcag gctgccgaac ggcatgttgt
     6061 cgcgcaggat ctcgcgctcc tcgccgtccg gcccggtgat cgaggtgagc gcgacgtgcg
     6121 cgccgggcgc cttcgccgcg tcggggatct cgatgtcgga cagcttgcgg cggccgaccg
     6181 cgtcctcctg ctgctcgacg gtgagcgcgt tccacgcccg catgtcgtgc aggtacttct
     6241 ggacgatcac gtagctgccg ccggcgaact cggcgtcctc gtcgccgatg acgacggcgg
     6301 cgcgggcgtc ctcgccggtc gggttctcgg tgccgtcgac gaagccgaga aggtcgcgct
     6361 gctcgaagta gcggaagccg tgcacctcgt ccacgacggt caccgcgccg gccagccggt
     6421 ccatgatctg cgcggccagc tcgaagcaga ggtccatctg gtgcgcgcgg atgtggaaca
     6481 gcaggtcgcc gggggtcgcg ggcgcggtgt gcacgccgcc gttcagctcg gtgaacgggt
     6541 gcagcccggc ggggcgcggg ccggagaaca gccggtccca ggcgtccgag ccgatgccgg
     6601 tgacgcaggt cagcccgccg cccggcaccc ggaagccgac cgcgcggacg aggccggcga
     6661 ggtcgggcag cagctcggcg accaccggct cgccgccggg gtcgacggtc accaccagga
     6721 agatcgccga gccggtcagc ggcccgagca cctcctggga cgcgccggcg cgcggggcgc
     6781 tcaccggcgg gcccgcgcgg gccgggtcgg gcgtgtcatg gccgtctcct gaactgatcg
     6841 cggtgtgcgg cacaccatta tccggtgcgc cctgggcccg aggtacgcca ggtgtctgca
     6901 atgtccatgt ttgtccggtg gcctgcggga agggcggaaa ggacaggtcc cgcgagggga
     6961 ggagcagagc catgagggca gtggtctggc acgacgtcgg cgagatcagc ctggacgagg
     7021 tgcccgaccc ggccgtccgc gaccccgagg acgcggtcgt ccggatcacc gccagcgcga
     7081 tctgcgggac ggacctgcac ttcgtccggg gcacgatgcc cgatatggaa ccgggcacgg
     7141 tcctcggcca cgaggccgtc ggcgtcgtcg aggaggtcgg ccgggacgtc cgcaacttcg
     7201 tgcccggcga ccgcgtcgtc atcccgtcca cgatcggctg cggccggtgc tcgtactgcc
     7261 gcgccggcta ctacgcccag tgcgacgtcg ccaacccgaa cggcccgtcc gccggctcgt
     7321 cgttctacgg cggcccgcgc tcgaccggcc cgttcgacgg cctgcaggcc gagtacgcgc
     7381 gcgtcccgta cgcgcacacc aacctcgtcg cgatcccgcc gggcgtcacc gacgagcagg
     7441 cgctgatggt cagcgacatc ctgccgaccg gctggttcgg cgcgcggctc gccgaggtcg
     7501 gccccggcga caccgtcgcc gtgctcggcg ccgggccggt cgggcagctc gccgtgctgt
     7561 cggcgcggat tcagggcgcc ggccgggtct tcgtcgtgga cggccacgcc gaccggctgg
     7621 agacggcccg gctgcagaac gccgagaccg tcgacttcaa caccgaggac ccggtcgcgg
     7681 tcgtgcgcga gatgaccggc ggcatcggcg cggaccgggt gatcgacgcg gtcggcgtgg
     7741 acgccgagag cccggcgagc ggccccgccg ccgaggcgat caccaaggag gaccggcgcg
     7801 agttcgagcg cgagcaggcg caggccgcgc cgcgcaccgg gcaggacggc gtccggtggc
     7861 gccccggcga cgcgcccggc gaggcgctgc gctgggcggt ggacatggtc gccaaggccg
     7921 gcaccatcgg gatcgtcggg gtgtacccgc cgcacttcac cagcttcccg ctgggcctcg
     7981 cgatgaaccg gaacctgacc gtcaaggcgg gcaactgcaa ccaccgccgg tacgtgccgg
     8041 ggctgctcag ccgcatcgcc aagggcggcg ccgacccgac caccatcctc acccagcagg
     8101 cgcgcctgcc cggggtcatc gaggcctacg aggcgttcga ccggcgcgag cccggctgga
     8161 cgaaggtggc gctggaggtc gccggatgac ggccggccgc gacagggggg agcacaccat
     8221 gaagatcgcc gatacgagga gggtcgagga gctgctgggc gacctggcgt tcccggccag
     8281 caaggagcgg atcgttgagc acgcgcacgc cgagcgcccg ctgagcgagg aggagcgggc
     8341 gctgtcgtcg ctgccgctcg gcacctacgg cagcgcggcg gaggtgctgc gctcgctgcc
     8401 gatcgacccc gacccgggcc gcaccccggc cgagcaggcc taccagcggc gccaccacaa
     8461 caagcagggc ctcgccgagc acatgcgcga caccgagcgc ccgcccgtcc aggacgagct
     8521 ccgcggcgac gacacccccc actggcaccc ctgacgaacc cgccggccgc gcagccaccc
     8581 gccaccgcgc gcgctcactc gccaccgcgc gcccgcgctc gctcacccgc gcgggcgcgc
     8641 gcgcccgcac ctggcgcggg cgcgcgtttg aagggtcttt ccccggtcat taccaagcgt
     8701 tggtagtgga cgaggagggg agcccggatg acggctgtgc agggcgcgcc ggacaccagt
     8761 acggcggacg cgctggcgcg ccaccgcaag gggaccaggg tcggcgacat cctcgcgacg
     8821 accgatcaca aaatggtcgg ctacctgtat ctcgggacct cgttcgcgat gttcctgatc
     8881 gccggcctga tggcgatgct catgcgggcc gagctcatgc agcccggcat gcaggtgatg
     8941 aaccgcctgc agtacaacca gctgttcacc attcacggca cgatcatgct gctgctgttc
     9001 gcgacgccgc tgttcgcggg gttcgccaat gtgatcgtgc cgctgcagat cggtgccccg
     9061 gacgtggcgt tcccgcggct gaacacgctc agctattacc tgttcctgtt cggcgccgtc
     9121 atggtgctgg cgggattcct gatgccgggc ggcgcggcgg cgttcggctg gttctcctat
     9181 tcgccgctgt cgtcgtcgac gtactcgccg ggactcggcg ccgacatgtg ggtgatgggg
     9241 ctgatcgtgt ccggattcgg gacgatcttc acctcggtga acctgatgac cacgatcatc
     9301 gcgatgcgcg cccccggcat ggtgatgttc cggctgccga tcttcacctg gaacgcgctg
     9361 ctgaccagcg tgatggtgct gatcgcgttc cccgtgctga ccgcggcgct gttcgcgctg
     9421 gaggcggacc gcaagttcgg cgcgcacatc ttcgacggcg cgaccggggg cgcgctgctg
     9481 tggcagcacc tcttctggtt cttcggccat ccggaggtct acatcgtggc gctgccgttc
     9541 ttcggcatcg tcaccgagat catcccggtg ttcgcgcgca agcccgtctt cggctacctg
     9601 ggcatggtgt gcgcgacgat cgcgatcacc ggcctgtcga tgaccgtctg ggcgcaccac
     9661 atgttcgcca cgggccaggt cctgctgccg ttcttctcca tgacgtcctt catgatcgcg
     9721 gtgccgaccg gcatcaagtt cttcaactgg gtcggcaccc tgtggaaggg gcacctcacg
     9781 ttcgagacgc cgatgctctg ggcgatcggg ttcctcgtga cgttcctgtt cggcggcctc
     9841 accggcgtga tcctggcgtc cccgcccatg gacttccacc tcaccgactc ctatttcgtc
     9901 gtcgcccatt tccattacgt gctgttcggc acggtggtct tcgcgatgtt cggcggcttc
     9961 tacttctggt ggccgaagat gaccgggaag aagctcgacg agacctgggg gaagatccac
    10021 ttctggtcgc tgttcgtcgg cttccacacc accttcctcg tccagcactg gctcggcgcc
    10081 gaaggcatgc cgcgccgcat cgcggactac tcgaagcagt tcgcgacgct gaacctcgtg
    10141 tcgtcgatcg gcgccttcat cctcggcgcg tccaccttcg cgttcctctg gaacatctac
    10201 cggacgcacc gccgcgcccg tcccgtcgag accgacgacc cgtggggcca cggcgcctcc
    10261 ctggagtggg cgacctcctg cccgccgccc cgccacaact tcgagacgat gccgcgcatc
    10321 cgttccttcc gtccggcatt cgacctgcac tacccgccga aggagccccc gcaggagtcc
    10381 gagccggccc ttccgcaacc ccccaccacc tgagacccgg aaccgacgac cgagcccggc
    10441 gcaccagaag gtgcgccggg ctccggcttg aggttctcgt ggggtgctag gaggtctcct
    10501 tcttctcctt ctccacggcg ccgttgcgct tgaggtcgcc ctgcccgccg ccgtgggact
    10561 cggtctggta gttgaggtag aaccggttgg tgcgggtccg gacctgcgtg cggacgcggc
    10621 cgggcgtcgg cagcgggatg atgtggtgcg ggtaggccgc gaccagctcg cggggctgcc
    10681 gggtctgcag gatggcggcc gcctccggcg cggaccgctc gtgccgctcg gtgtagccgc
    10741 cctccggggt cagcgagatc agcccgctct ccacgccctc ctcgcgtttc tgcag
//
