LOCUS       NZ_CP053892            10831 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Actinomadura verrucosospora strain NRRL:B18236 chromosome.
ACCESSION   NZ_CP053892
VERSION     NZ_CP053892.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M.
  TITLE     Actinomadura verrucosospora NRRL-B18236 (PFL_A860) Genome sequencing
            and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2016
            Assembly Method                   :: Newbler v. 3.0
            Assembly Name                     :: A860_AS2.mpe1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013211575.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,277
            CDSs (total)                      :: 9,181
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 96
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 18 of 71
            Pseudo Genes (incomplete)         :: 57 of 71
            Pseudo Genes (internal stop)      :: 2 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:15:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9359853
            Orig. end                         :: 9370684
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Peter Leadlay,
            NRRL-B18236 (patent collection, not in online catalogue).
FEATURES             Location/Qualifiers
     region          1..10831
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="29"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10831
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10831
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5831](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5831
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    201..216
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.55"
                     /tool="antismash"
     misc_feature    252..267
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            complement(286..771)
                     /locus_tag="ACTIVE_RS42170"
                     /old_locus_tag="ACTIVE_8460"
     CDS             complement(286..771)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023202.4"
                     /locus_tag="ACTIVE_RS42170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8460"
                     /product="Lsr2 family protein"
                     /protein_id="WP_246342524.1"
                     /transl_table=11
                     /translation="MPIRAPIRRAFSRAAPRRDVMVGRGIRYGQGRRVGEGKEHKVQMA
                     EKVIRVDDIDGSEGDDVAKRDFEVLGRTFTIDLSEDNFKRLNEMLDALAPFIENAAEVK
                     PAGKGRKSRVAKIKGYTNGDVREWALRDGVEVSERGKISDEVYAAFIDAHPDAKPDA"
     gene            complement(792..2102)
                     /locus_tag="ACTIVE_RS42175"
                     /old_locus_tag="ACTIVE_8461"
     CDS             complement(792..2102)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653216.1"
                     /locus_tag="ACTIVE_RS42175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8461"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_173100193.1"
                     /transl_table=11
                     /translation="MTTAYEDARTGSPVLDGLTEEPWRDVPAEEARWMRPRLPALLGPM
                     VQGVLRHVPEYDRPGDTAYVRVTEAAVAHAMEHFVQMIADPDASWKEVYQVFFDVGYGE
                     AVEGRSLEHLQNAMRVASRLAWRHLAVEADRLGRPRALVSLLAEANFAYLDALASAAAH
                     GYARAREKAAGEREQRRGRLLSLLLSDPAAPPEVVGEQSARAGWPLPRRLAVIVLRPRP
                     GSGGEGPAGLPPSLLSGLDHGRPCLVMPDPDGPGRMDELTATLAGWTGAVGPSVPADQA
                     RTSLQWARRALDLAPAAASRGGDGAAEGGTAGGAARRAGRGSRRGGLLVRADAHLPELL
                     LQEGGSLAALVAARRLAPLEAAGARQGVRLAETLLECMKHGFNATGAAEVLCVHPQTVR
                     YRLAQLHEMFDFDIEDPELRLEMMLLLSTWIRARSSS"
     gene            2355..4451
                     /locus_tag="ACTIVE_RS42180"
                     /old_locus_tag="ACTIVE_8462"
     CDS             2355..4451
                     /EC_number="1.11.1.6"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653884.1"
                     /locus_tag="ACTIVE_RS42180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8462"
                     /product="catalase"
                     /protein_id="WP_173100194.1"
                     /transl_table=11
                     /translation="MANPAGHEDRKQEQLERRRVGPEGAAGLTTDQGVRVDDTDNSLTV
                     GERGPSLMEDFHLREKITHFDHERIPERVVHARGSGAYGQFTLTESLADYTTAEFLCDT
                     SLTTPVFVRFSTVAGSRGSADTVRDVRGFATKFYTNQGNFDLVGNNMPVFFIQDGIKFP
                     DFVHAVKPEPPNEIPQASSAHDTLWDFVQLQPETMHMMMWLMSDRAIPRSYAMMQGFGV
                     HTFRLVNAEGRATFVKFHWRPKLGTHSLAWDETQKIAGKDPDFNRRDLWDRIDDGDFPE
                     YELGVQLVAEEDEHKFGFDLLDPTKIIPEEEVPLRTVGRMVLNRNPDNFFAETEQVAFH
                     IGNVVPGIDFTNDPLLQARLFSYLDTQLIRLGGPNFPQIPVNQPVADVRNHQRDGYHQM
                     KLHQGQASYHKNSLGGGCPAVGGPGSYTHYQERVDGDKIRKRSESFKDHYSQATLFWNS
                     MADWEKEHIVGAFRFELGKCDHKHVREGVVAQLNHVDHDLAVQVAEGVGVEPPADAPTA
                     NHGRTSPALSQDDQPGSVQGRKVAVLVADGVDSAAVGAVGDALAEAGAVVDVLAAKDGH
                     VQAANGAQVTVTRAMPTVASVLYDAVLVPGGEASVRTLAQDGDAVHFVAEAFVHCKAIG
                     ALDEGVALLERAGITGVKTSENGTVEDAGVVTGRGPGGDFSAELITAIAKHRHWRRQKD
                     HVPA"
     misc_feature    complement(4569..4584)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(4613..4627)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.83"
                     /tool="antismash"
     misc_feature    complement(4615..4628)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 11.86"
                     /tool="antismash"
     misc_feature    4617..4630
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.81"
                     /tool="antismash"
     misc_feature    4617..4633
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.34"
                     /tool="antismash"
     misc_feature    complement(4620..4633)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.8"
                     /tool="antismash"
     misc_feature    complement(4885..4901)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.35"
                     /tool="antismash"
     misc_feature    4911..4925
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            5001..5831
                     /locus_tag="ACTIVE_RS42185"
                     /old_locus_tag="ACTIVE_8463"
     CDS             5001..5831
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="ACTIVE_RS42185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8463"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_173100195.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-06, bitscore: 27.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAASVIERFRELVVDESDPFYFDHPLDHVPGMLLFSGVLDLAVRA
                     DSARSRGRRAGRVAAGLDFTRFCELDRGTTLACRPVPGRNRAWTVAARQDAREVCAGSI
                     AFTDDTACAGGAGVAAPPGRARPPAAIPPSRHAPADLVHRYRPENILVGEPVRRGRGVV
                     EAAVLRPPGRARDAWTPVEIVEAGRQFATLLEHSEHGRPMDARLLWVSLRMDVPFRLDG
                     SVPLLLRWPVARVPGPRSRCAAVLADASTGAGLGSLAYEAYAVDQERYERTRSS"
     gene            5828..6469
                     /locus_tag="ACTIVE_RS42190"
                     /old_locus_tag="ACTIVE_8464"
     CDS             5828..6469
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /locus_tag="ACTIVE_RS42190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8464"
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="WP_173100196.1"
                     /transl_table=11
                     /translation="MTGLLRGRVLLLVPRAAFAAGAGVLTAGIAVHAARGGAAPAAMLA
                     LYLGWLLLEAPVTFRRAAASPADARTLPPYALARLLLVASAALGPFPWAGSSAWSAIPA
                     LLFVAGVALRWTAIRTLGRFYSHHVMRQDGHRVVTGGVYRAVRHPAYAGMLLANLAFTC
                     FFLTPLSAAAFVLLVAAVAWRIRVEERAMWDVPGYPPYAAGRARVLPKVW"
     gene            6490..9192
                     /locus_tag="ACTIVE_RS42195"
                     /old_locus_tag="ACTIVE_8465"
     CDS             6490..9192
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /locus_tag="ACTIVE_RS42195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8465"
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_173100197.1"
                     /sec_met_domain="PPDK_N (E-value: 4.7e-44, bitscore: 150.0,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSEVGHKFARLATLRDAGFPVPEFFCVPASEFDRALDGLSGAL
                     PPRSAVPAAQWCRFAASALAGAVPDGALAGRLLAGFDELTRGGAGMGAADGTVAVRACV
                     VPGPDGAAEDSAGDPFAGLSDSFLYVRRDGLLEAVARCWASAFKTEAVRYREFRGIDPA
                     AARVAVGVQRMVPGTRSFVAFTRDPRDGAHRHVIAAAHGIGEGVVQEKADVDHFFVDPG
                     TGRVEAEVVTKDRMVGPPADGGEGPRTVRVPDELRTPPVLTGEQAGEVADLAARVEVLF
                     GCPQDIEGTITPDGAVHLVQARPLAAAPEQEREPRVHWGDHNITESFPGVSGALTYSQA
                     RAFYQLAFTDLYRRMGVPARRLRARGHRLARMVGHLDGRVYYRLDDWQDLHGQVPVFDL
                     VRSGWEEGMGITGEARAEHRWPRARVAAALPALAWRAAGHRRATVRFLRWWDALMADVR
                     GPASRPLAEYTPDELITLYRWVWAEASVRWGVTLANGVYGLLLMRAATALLRRWAGAEP
                     ELLPGLLCGGPENRSLAAARAALALAERAAGVPALKAALLADDLGGRTDEEHLRAVWDD
                     VAAGRHGAFLAASARAYLRRYGDRAVHDLKLDEPTPRQRPWMTAAVLRPLVRQGATAAA
                     NRAAEAREAAAARRRLRESCPGPAKRAVLRVLLGMMRWSVRAREDARFCRTQLFGLSRE
                     VMWRLGGELAAAGLLDAPLDVADLTVEEVIGAFDGTLPGCDLRGLAALRRAERVRNGAA
                     PPPPALLSVPAARPAAAALPYARPLGEPDGAAADGTLRGLGSGGGTVRGRAKVVLDPRV
                     PPGDCAGRILVARETDPGWLALMIAASGLVVERGTLLSHTAVTGRLLGVPTAVAVGGAV
                     ARIPDGAWIELDGRSGTIRILGAPAGAAG"
     misc_feature    6529..6542
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.22"
                     /tool="antismash"
     gene            9238..10284
                     /locus_tag="ACTIVE_RS42200"
                     /old_locus_tag="ACTIVE_8466"
     CDS             9238..10284
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /locus_tag="ACTIVE_RS42200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8466"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_173100198.1"
                     /transl_table=11
                     /translation="MEAGAFGQAAQERAAAEVEAEIGRLCERLAEGLAMGLDEGREMVG
                     GAMAEFLVDFFETVRAKGSRPGMRGMVALPMLVHGAETGDPAPARPLAVVHLLWWAAAR
                     YLDDLTDVPGAPPAGNTAVAKRVLTALAVGGQLPARLVADLPVPDAVRCGLAGEVSRAW
                     LDAVDGQLRDLTDRPSAATPASVLRGYAGKTGAPYAMAAASAARLAGAGDDRAGGWRAF
                     GRRLGVLRQLVNDQRDLASGRHEDLANGTATYLLAHLLSALPAGRRREALALHAGARHS
                     ACARAELTAWMLDDDVLDGYAASVAPLIGHAHDLLGLLGGDPAFVRELHDLVDETAGRL
                     PGLRLAVA"
ORIGIN
        1 tgatgccgtg gttcttagcc cactcgcgaa tctcggcgga ccgctcgcgg ctgcccgcgg
       61 tgcgcgtgcc gcgcacctgg cgcccggcgg gcttgcgcgc cttgcgggca ccggccacga
      121 acggctccag cccgtcgcgc agcttcgcgg cgttcttctt gctcaggtcg atctcgtaag
      181 tggtgccatc gagcgaaaag ctgaccgtct cgtcggcctc gccaccgtcg atgtcgtcca
      241 cgagaaggac ttcgaccttc tgtgccatgc ggggactccc tctagctatg cgtccggctt
      301 cgcgtccgga tgagcgtcga tgaatgcagc atagacctca tccgatatct tgccacgttc
      361 ggagacttcg acgccgtcgc gcaatgccca ctcgcggaca tcgccgttcg tgtagccctt
      421 gatcttcgcg acccgggact tgcggccctt cccggcgggc ttcacctcgg cggcgttctc
      481 gatgaagggc gcgagcgcgt ccagcatctc gttcagccgc ttgaagttgt cctcgctgag
      541 gtcgatcgtg aacgtccgac ccaggacctc gaagtcccgc ttggcgacat cgtccccttc
      601 ggagccgtcg atgtcgtcga ctcgaatgac cttctcggcc atctgtacct tgtgttcctt
      661 cccctcgcct acgcggcgcc cctgaccgta gcggattccg cgccccacca tgacgtcgcg
      721 gcgcggcgcg gcacgggaaa aggcgcggcg gatcggcgcg cggatcggca ctccgttcgc
      781 gcgggcggcg ctcaggaaga ggaacgggcc cggatccagg tgctgagcag cagcatcatc
      841 tccaggcgga gttccgggtc ctcgatgtcg aagtcgaaca tctcgtggag ttgcgcgagc
      901 cggtaccgga cggtctgcgg gtgcacgcac agcacttcgg cggcgcccgt ggcgttgaag
      961 ccgtgcttca tgcactccag cagcgtctcc gccagccgga cgccctgccg ggcgcccgcg
     1021 gcctccagcg gcgccagccg ccgcgccgcc accagcgcgg cgagcgagcc gccctcctgc
     1081 agcagcagtt ccggcaggtg cgcgtcggcg cggacgagca gcccgccgcg ccgcgacccg
     1141 cggcccgccc tcctcgccgc gccgccggcc gtcccgccct ccgcggcgcc gtccccgccg
     1201 cgcgaggccg ccgcgggcgc gaggtcaagg gcgcgccgcg cccactgcag cgacgtccgc
     1261 gcctggtcgg cggggacgga cgggccgacc gcgcccgtcc agccggccag cgtcgcggtc
     1321 aactcgtcca tccggccggg gccgtccggg tccggcatca ccaggcaggg gcggccgtgg
     1381 tccagcccgg acagcagcga cggcggcagc ccggcggggc cctcgccccc gctgcccggg
     1441 cgcggccgca gcacgatcac cgcgagccgg cgcggcagcg gccagcccgc ccgcgcggac
     1501 tgctcgccga cgacctcggg cggcgcggcc gggtccgaca gcagcagcga cagcagccgg
     1561 ccgcggcgct gctcgcgctc cccggcggcc ttctcccggg cccgcgcgta gccgtgcgcc
     1621 gccgcggacg cgagcgcgtc caggtaggcg aagttcgcct cggcgagcag gctgaccagg
     1681 gcgcgggggc ggccgagccg gtccgcctcg acggccaggt gccgccaggc cagccgcgag
     1741 gcgacccgca tcgcgttctg caggtgctcc aggctgcgcc cctcgaccgc ctcgccgtac
     1801 ccgacgtcga agaagacctg gtacacctcc ttccaggagg cgtccgggtc ggcgatcatc
     1861 tggacgaagt gctccatcgc gtgcgcgacc gcggcctccg tcacgcggac gtaggcggtg
     1921 tcgccgggac ggtcgtactc cgggacgtgc cgcagcacgc cctgcaccat cgggccgagc
     1981 agggcgggga gccgcgggcg catccagcgc gcctcctcgg cgggcacgtc ccgccacggc
     2041 tcctccgtca gcccgtccag caccggagac ccggtgcgcg cgtcctcgta tgccgtcgtc
     2101 atggcgacac ctcctcgacc gaacgcccgg tcgtctcaac ctaggcatgc ggggcggggc
     2161 gcgacactca tccgtccgca ccgccggaag gcggtgattg tcagccgttt gacaagcccc
     2221 cgcgcgcggc ggcggacgtg cggaggcggc gcggcggccg tgctcaagtt ccggtcaaag
     2281 cgcgcatggc ggagggttcg ccgggtagcg gccccaccga atgcgatcac cgagcagtca
     2341 cgaggagccg aatcatggcc aacccggccg gccacgagga ccggaagcag gagcagttgg
     2401 agcggcggcg ggtgggcccg gagggggcgg ccgggctgac caccgaccag ggcgtccggg
     2461 tcgacgacac cgacaactcg ctgacggtgg gcgagcgggg cccgtcgctg atggaggact
     2521 tccacctccg cgagaagatc acccacttcg accacgagcg gatccccgag cgggtcgtgc
     2581 acgcgcgcgg ctccggcgcg tacgggcagt tcacgctgac cgagtcgctg gccgactaca
     2641 ccaccgccga gttcctgtgc gacacctcgc tgacgacgcc ggtgttcgtg cggttctcga
     2701 ccgtcgccgg gtcgcgcggg tcggccgaca ccgtccgcga cgtccgcggc ttcgccacga
     2761 agttctatac gaaccagggc aacttcgacc tcgtcggcaa caacatgccg gtcttcttca
     2821 tccaggacgg catcaagttc cccgacttcg tgcacgcggt gaagcccgag ccgccgaacg
     2881 agatcccgca ggcgtcgtcc gcgcacgaca ccctgtggga cttcgtgcag ctccagcccg
     2941 agacgatgca catgatgatg tggctgatgt cggaccgggc gatcccgcgc agctacgcga
     3001 tgatgcaggg cttcggcgtg cacacgttcc ggctggtgaa cgccgagggc cgcgccacgt
     3061 tcgtgaagtt ccactggcgg ccgaagctcg gcacccactc gctcgcctgg gacgagacgc
     3121 agaagatcgc cggcaaggac cccgacttca accggcgcga cctgtgggac cgcatcgacg
     3181 acggcgactt ccccgagtac gagctcggcg tccagctcgt ggccgaggag gacgagcaca
     3241 agttcgggtt cgatctgctc gaccccacca agatcattcc ggaggaggag gtgccgctgc
     3301 ggacggtcgg ccggatggtc ctgaaccgca accccgacaa cttcttcgcc gagaccgagc
     3361 aggtcgcctt ccacatcggc aacgtcgtgc cgggcatcga cttcaccaac gacccgctgc
     3421 tgcaggcccg gctgttctcc tacctggaca cccagctgat ccggctcggc ggtccgaact
     3481 tcccgcagat cccggtgaac cagcccgtcg ccgacgtccg caaccaccag cgcgacggct
     3541 accaccagat gaagctgcac caggggcagg cgagctacca caagaactcg ctcggcggcg
     3601 gctgcccggc ggtcggcggg cccggctcct acacccacta ccaggagcgc gtggacggcg
     3661 acaagatccg caagcgcagc gagagcttca aggaccacta ctcgcaggcg acgctgttct
     3721 ggaacagcat ggccgactgg gagaaggagc acatcgtcgg cgcgttccgg ttcgagctcg
     3781 gcaagtgcga ccacaagcac gtccgcgagg gcgtcgtcgc gcagctcaac cacgtcgacc
     3841 acgacctcgc cgtgcaggtc gccgagggcg tcggcgtcga gccgccggcg gacgccccga
     3901 ccgccaacca cggccgcacc tcgcccgcgc tcagccagga cgaccagccc ggctccgtcc
     3961 agggccgcaa ggtcgcggtc ctggtcgccg acggggtcga ctcggcggcc gtcggcgcgg
     4021 tcggcgacgc gctggccgag gcgggcgcgg tggtcgacgt gctcgccgcc aaggacgggc
     4081 acgtccaggc cgccaacggc gcgcaggtca cggtgacccg cgcgatgccg accgtcgcgt
     4141 ccgtgctgta cgacgcggtg ctggtgcccg ggggagaggc gagcgtgcgg acgctggcgc
     4201 aggacggcga cgcggtgcac ttcgtcgcgg aggcgttcgt gcactgcaag gcgatcggtg
     4261 cgctcgacga gggcgtcgcg ctgctggagc gcgccgggat caccggggtg aagacctccg
     4321 agaacggcac cgtcgaggac gccggggtcg tcaccgggcg cggtcccggc ggcgacttca
     4381 gcgccgagct gatcacggcg atcgccaagc accggcactg gcgccggcag aaggaccacg
     4441 tcccggcgta acgggaccgg cgccgcggca ggggcccggc gcggtggcgc gcttcgagcg
     4501 cgcaccgcgc cgggccctgc cgcgtgtggc cgcaccctgc cgcgtgtggc cgcattccgc
     4561 caatcaattg tgggccacgt cacaaagcgg gcgggcggcg gaatgcggga agcgtgatct
     4621 gaaaatatat cttgtgcgac tcgccggtga aaacgcgcgg ccgtgaatcg cgggaatcgc
     4681 gcgcattccg cccgcattcc ggctggacgc ggccgggccg ccgccgggcg gacgtcgcga
     4741 ccgtccctgc aagtgggtcc tgacatgcgg attccgggcg gggcgcggcc gtcccgccgc
     4801 gcgtccggcg ggccatgcct tgccggtctg ggaagacccg cacggcccgg actgcccacg
     4861 ggtgcagttc atccgggcgc aattctgtag caagatgacg gccgtcccgc tgaaattcct
     4921 gtgcttccgg taagggcgcc gggcggcttt tccgacggcc gccgggcaag ggcatgggtc
     4981 cgattatgga gggagggcgc atggccgcgt ccgtcattga acgattccgc gagctggtcg
     5041 tcgacgagtc cgatcccttc tacttcgacc accccctcga ccacgtgccg ggaatgctgc
     5101 tgttcagcgg cgtgctggac ctcgccgttc gcgcggactc ggcgcggtcg cgcggacggc
     5161 gggccggccg ggtggcggcc gggctggact tcacccggtt ctgcgagctc gaccgcggca
     5221 ccaccctggc ctgccgcccc gtccccggcc gcaaccgcgc ctggacggtc gccgcccggc
     5281 aggacgcccg cgaggtgtgc gcgggctcca tcgcgttcac cgacgacacc gcatgcgccg
     5341 gcggcgccgg cgtggccgcg ccgccgggcc gggcacggcc gcccgccgcg atcccgccgt
     5401 cccggcacgc gcccgcggac ctcgtgcacc ggtaccgccc ggagaacatc ctggtgggcg
     5461 agcccgtgcg gcgcgggcgc ggcgtggttg aggcggcggt gctccggccg ccgggccggg
     5521 cgcgggacgc ctggaccccc gtggagatcg tcgaggcggg ccgccagttc gcgacgctgc
     5581 tggagcacag cgagcacggc aggccgatgg acgcgcgcct gctgtgggtg tcgctgcgga
     5641 tggacgtccc gttccggctg gacggatcag tcccgctgct gcttcgctgg ccggtcgcgc
     5701 gggtgccggg gccgcgatcg cggtgcgcgg ccgtcctcgc ggacgcctcg acgggcgcgg
     5761 gcctcgggtc gctggcctac gaggcgtacg ccgtcgacca ggagcgctac gagcggaccc
     5821 ggtcctcatg accggtctcc tccgcggaag agtcctgctg ctcgtgccgc gcgccgcgtt
     5881 cgccgccggc gcgggcgtcc tgaccgccgg gatcgccgtg cacgcggcgc gcggcggcgc
     5941 cgcgccggcg gccatgctcg cgctgtacct gggatggttg ctgctggagg cgccggtgac
     6001 gttccgccgg gccgccgcgt ccccggcgga cgcgcggacg ctcccgccgt acgcgctcgc
     6061 ccgcctgctg ctggtcgcgt ccgccgcgct cgggccgttc ccgtgggccg ggtcgtcggc
     6121 gtggtccgcg atccccgcgc tgctcttcgt cgccggggtc gcgctgcggt ggaccgcgat
     6181 ccgcacgctc ggccggttct actcgcatca cgtgatgcgg caggacggcc atcgcgtcgt
     6241 gaccggcggc gtctaccggg ccgtccggca cccggcgtac gcgggcatgc tgctggccaa
     6301 tctcgccttc acctgcttct tcctcactcc gctcagcgcg gccgcgttcg tgctgctcgt
     6361 ggcggcggtc gcctggcgga tccgcgtgga ggaacgggca atgtgggacg ttccgggcta
     6421 cccgccgtac gccgccgggc gggcgcgcgt gctgccgaag gtgtggtgag cggcgtgccc
     6481 tccggatcga tgagcacgag cgaggtcggg cacaagttcg cccggctggc cacgctgcgc
     6541 gacgcgggct tcccggtgcc ggagttcttc tgcgtgcccg cgtcggagtt cgaccgggcg
     6601 ctggacggcc tgagcggcgc cctgccgccg cgctccgcgg tcccggcggc gcagtggtgc
     6661 cggttcgcgg cgtcggcgct ggcgggggcc gtcccggacg gcgccctcgc cggccggctg
     6721 ctcgcgggtt tcgacgagct gacgcgcggc ggcgcgggca tgggcgccgc ggacgggacg
     6781 gtcgccgtgc gcgcgtgcgt cgtccccggc ccggacggtg cggccgagga ttcggccggc
     6841 gacccgttcg ccgggctgag cgacagcttc ctgtacgtcc gccgcgacgg gctgctggag
     6901 gcggtggcgc gctgctgggc gtcggcgttc aagaccgagg cggtccgcta ccgggagttc
     6961 cggggcatcg acccggcggc ggcgcgggtc gcggtcggcg tccagcggat ggtgccgggg
     7021 acccgctcgt tcgtcgcctt cacccgggac ccccgggacg gggcgcaccg gcacgtcatc
     7081 gccgccgcgc acggcatcgg cgagggcgtc gtccaggaga aggccgacgt cgaccacttc
     7141 ttcgtcgatc ccggcaccgg gcgggtggag gccgaggtcg tgaccaagga ccgcatggtc
     7201 ggcccgccgg cggacggcgg tgaaggcccg cggaccgtcc gcgtcccgga cgaactgcgc
     7261 acgccgcccg tcctcaccgg cgagcaggcc ggggaggtcg ccgacctcgc ggcccgcgtc
     7321 gaggtgctgt tcgggtgccc gcaggacatc gaggggacga tcacgccgga cggcgccgtc
     7381 cacctcgtcc aggcgcgccc gctggccgcc gcgccggagc aggagcggga accgcgcgtc
     7441 cactggggcg accacaacat caccgagagc ttccccggcg tgtccggcgc gctgacctac
     7501 tcgcaggcgc gcgccttcta ccagctcgcc ttcaccgacc tctaccggcg gatgggcgtg
     7561 ccggcgcggc ggctgcgcgc ccgcggccac cggctcgcgc ggatggtcgg gcacctcgac
     7621 ggccgcgtct actaccggct ggacgactgg caggacctgc acggccaggt gcccgtcttc
     7681 gacctggtcc ggtccggctg ggaggagggg atgggcatca ccggcgaggc gcgcgcggag
     7741 catcggtggc cgcgcgcccg cgtcgccgcc gcgctgcccg cgctcgcctg gcgcgccgcc
     7801 gggcaccgcc gcgccaccgt ccggttcctg cgctggtggg acgcgctgat ggcggacgtc
     7861 cgcggccccg cgtcccgccc gctcgccgag tacaccccgg acgagctgat caccctgtac
     7921 cggtgggtgt gggccgaggc gtccgtgcgc tggggcgtca cgctcgccaa cggcgtctac
     7981 ggcctgctgc tcatgcgggc ggccaccgcg ctgctgcgcc gctgggcggg cgccgagccg
     8041 gagctgctgc ccgggctgct gtgcggcggg ccggagaacc ggtcgctcgc cgccgcccgc
     8101 gccgccctcg cgctggccga gcgcgccgcc ggggtgcccg cgctgaaggc ggcgctgctg
     8161 gccgacgacc tgggcggacg caccgacgag gagcatctgc gggccgtctg ggacgacgtc
     8221 gccgcgggcc ggcacggcgc gttcctcgcc gcgtccgcgc gtgcctacct gcgccgctac
     8281 ggcgaccggg cggtgcacga cctcaagctc gacgagccca cgccgcggca gcggccctgg
     8341 atgaccgccg ccgtcctgcg gccgctcgtc cggcagggcg cgacggccgc cgcgaaccgg
     8401 gccgccgagg cgcgggaggc ggcggccgcg cggcgccggc tgcgcgaatc ctgccccggc
     8461 ccggccaaac gcgccgtcct gcgcgtactg ctcggcatga tgcgctggtc agtgcgcgcg
     8521 cgggaggacg cgcggttctg ccgcacgcag ctgttcgggc tgtcccgcga ggtgatgtgg
     8581 cggctcggcg gcgagctcgc cgccgccggg ctgctcgacg ccccgctcga cgtcgccgac
     8641 ctcaccgtcg aggaggtcat cggcgcgttc gacggcaccc tgcccggctg cgacctgcgc
     8701 ggtctcgccg cgctgcggcg cgccgaacgc gtccggaacg gggccgcgcc gcccccgccc
     8761 gcgctgctgt cggtgcccgc cgcgcgcccg gccgccgccg cgctgcccta cgcgcgcccg
     8821 ctcggcgagc cggacggcgc cgccgcggac ggcacgctgc gcgggctcgg gtccggcggc
     8881 gggacggtcc gcggccgcgc caaggtcgtg ctggatccgc gcgtcccgcc cggcgactgc
     8941 gcgggccgca tcctcgtcgc ccgcgagacc gacccgggct ggctcgccct gatgatcgcc
     9001 gcgtccgggc tggtcgtcga gcgcggcacc ctgctctcgc acaccgccgt cacggggcgg
     9061 ctgctcggcg tgccgaccgc ggtcgcggtc ggcggcgccg tcgcccgcat ccccgacggc
     9121 gcctggatcg agctggacgg gcggtccggc acgatccgga tcctcggcgc cccggcgggc
     9181 gccgccggct gaccccggaa ccgcccgaga accccgcgaa caaggaggag tcggcacgtg
     9241 gaagcaggag cgttcggcca ggccgcgcag gagcgggcgg ccgccgaggt cgaggcggag
     9301 atcggccggc tgtgcgagcg gctcgccgag ggcctggcga tgggcctgga cgagggccgc
     9361 gagatggtcg gcggcgcgat ggcggagttc ctggtggact tcttcgagac cgtccgcgcc
     9421 aagggctcgc gccccggcat gcgggggatg gtcgcgctgc cgatgctggt gcacggcgcg
     9481 gagaccggcg acccggcgcc cgcccggccg ctcgccgtcg tccacctgct gtggtgggcc
     9541 gcggcccgct acctggacga cctcaccgac gtgcccggcg ccccgccggc cggcaacacc
     9601 gcggtcgcca agcgggtgct gaccgcgctg gccgtcggcg ggcagctgcc cgcccggctc
     9661 gtcgcggacc tgccggtgcc cgacgccgtc cgctgcggcc tcgccggcga ggtgtcgcgg
     9721 gcctggctgg acgcggtgga cggccagctg cgcgacctca ccgaccgccc gtccgccgcg
     9781 acgcccgcgt cggtgctgcg cggctacgcg ggcaagacgg gcgcgccgta cgcgatggcg
     9841 gcggcgtccg cggcgcgcct cgccggcgcc ggcgacgacc gggccggcgg ctggcgcgcg
     9901 ttcggccgcc gcctcggcgt gctccgccag ctcgtcaacg accagcgcga cctcgcgtcg
     9961 ggacggcacg aggacctcgc caacggcacc gccacgtacc tgctggcgca cctgctgtcc
    10021 gcgctgcccg ccggaaggcg ccgggaggcg ctcgcgctgc acgccggcgc ccgccactcg
    10081 gcctgcgcgc gcgccgagct gaccgcctgg atgctcgacg acgacgtcct ggacggctac
    10141 gccgcgtccg tcgcgccgct catcgggcac gcgcacgacc tgctcggcct gctcggcggc
    10201 gaccccgcgt tcgtccggga gctgcacgac ctcgtcgacg agaccgccgg gcgcctgccc
    10261 gggctccggc tggccgtggc ctgagaggag gaccccgcat gacttcggcc ggcggactgc
    10321 cggacttcct cgccgcgcag gccccgcggg tcgccgcggc ggtcggcgac gcgctcgccg
    10381 ggcacccggc gcaccagccg atccggcgca ccgccgggcc gctgttcggc ggccgggagg
    10441 agaccgccgc gcggctgctc gccctcgccg cggcgggctc gccgctgccc gccgacgacc
    10501 tcgcccggtt ccgcgacctc ggcgtgatgg cggcccggca gggcatcccg ctgccggtgc
    10561 tgtcggaggt gtgggacgtc gcgctcgccg cggccgcgcg gacctgctgg gcggcggtgc
    10621 ccgccgggcg cttcaccgag ctggccgagg cgacggggcg ggccgcgcgg ctcgcgggac
    10681 gggcccggga ggcgtgcatc caggggtacg cggacgcgcc gcgcgagggc gcccggtccc
    10741 ggccgctgcg ccggctgctc gccgagacgc tgatcgacgg cgcgcccgcc gaggtcatcg
    10801 cggaggccgc cggggtcgcg ctcgcccccg g
//
