LOCUS       NZ_CP053892            10810 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Actinomadura verrucosospora strain NRRL:B18236 chromosome.
ACCESSION   NZ_CP053892
VERSION     NZ_CP053892.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M.
  TITLE     Actinomadura verrucosospora NRRL-B18236 (PFL_A860) Genome sequencing
            and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2016
            Assembly Method                   :: Newbler v. 3.0
            Assembly Name                     :: A860_AS2.mpe1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013211575.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,277
            CDSs (total)                      :: 9,181
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 96
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 18 of 71
            Pseudo Genes (incomplete)         :: 57 of 71
            Pseudo Genes (internal stop)      :: 2 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:15:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9014153
            Orig. end                         :: 9024963
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Peter Leadlay,
            NRRL-B18236 (patent collection, not in online catalogue).
FEATURES             Location/Qualifiers
     region          1..10810
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10810
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10810
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5810](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5810
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(508..523)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.04"
                     /tool="antismash"
     misc_feature    523..538
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.25"
                     /tool="antismash"
     misc_feature    828..848
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 18.87"
                     /tool="antismash"
     gene            1280..2014
                     /locus_tag="ACTIVE_RS40595"
                     /old_locus_tag="ACTIVE_8146"
     CDS             1280..2014
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654832.1"
                     /locus_tag="ACTIVE_RS40595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8146"
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_173099921.1"
                     /transl_table=11
                     /translation="MLWFWILLRIVLSPIMYGAWWPRNRGMRNVPKRGPALLACNHQSF
                     ADHFFGPLPLMRPIFFIGKGEYFTGRGIKGLISKAFFTGVGVVPVDRTGGAEADAALQT
                     GLRILGEGKLLGIYPEGTRAPDERLYRGKTGVARLALKSRVPVIPMAMINTFRLMPPGQ
                     PYPRLGVRPGVRFGEPMDFSRYFGQEDDREVLRTVTDEIMKAIEKLSGQEYVDRYAAEV
                     KAEMAGKTVRPVEDGDEGIAED"
     gene            2019..3227
                     /locus_tag="ACTIVE_RS40600"
                     /old_locus_tag="ACTIVE_8147"
     CDS             2019..3227
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557771.1"
                     /locus_tag="ACTIVE_RS40600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8147"
                     /product="DUF5931 domain-containing protein"
                     /protein_id="WP_216858128.1"
                     /transl_table=11
                     /translation="MADDTTGGTTGLDAALWRSVAVYRALALVYAAVVIGKNSGGYAHP
                     LGGWAVLAVLTVWTGVATLAYRDPARRGWPLLAADLAVAFGCILATAWVETAPHIAEGR
                     PTLPVSWVAAAVLAWAVEGGRRIGVTAALVLGVANLLVHAFADTAGGMGNTTLNGIVLL
                     FLAGLVVGQVVRLAREAEARLARAVELEAATRERERLARRIHDSVLQVLAMVQRRGGEA
                     GGEAAELGRLAGEQEAALRALIGAGPASAAGPLPAGVPAPRAGAGRSTDLRPLLTGQAS
                     AAVTVSTPATPVPLPEHDAHEIAAAVAAALDNVRRHCGDGARAWVLLEDGPGEVTVSVR
                     DDGPGMPPGRLDAAAADGRLGVAQSIRGRVRDLGGTVSIVSAPGEGTEIEMTVPRSSAA
                     FKV"
     gene            3230..3892
                     /locus_tag="ACTIVE_RS40605"
                     /old_locus_tag="ACTIVE_8148"
     CDS             3230..3892
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154136.1"
                     /locus_tag="ACTIVE_RS40605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8148"
                     /product="response regulator transcription factor"
                     /protein_id="WP_216858129.1"
                     /transl_table=11
                     /translation="MSEVALRVMVVDDHPMWREAVARDLTEAGHEVVATAGEGRTAVRI
                     AAAARPRVAVVDLQLPDISGVEVTRHLAAADPPVRVLVLSASGEQQDVLEAVKAGATGY
                     LLKSAGREEFLDAVRRTADGDAVFTPGLAGLVLGEYRRLAAAPAREDDDAPRLTDRETE
                     VLRLVAKGLTYKQIAQRLVLSHRTVQNHVQNTLGKLQLHNRVELVRYAIEKGLDEED"
     gene            3983..4999
                     /locus_tag="ACTIVE_RS40610"
                     /old_locus_tag="ACTIVE_8149"
     CDS             3983..4999
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ACTIVE_RS40610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8149"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_173099922.1"
                     /transl_table=11
                     /translation="MDETLWLLTLHFLVGLVLVAVLWRRRSALLRGTPPTRDLHPYEIA
                     YLQGGGRHAIAASLAALRLDGAVDAHGDGRMAAAPPPSDAPRTAGHPLDGAVFGAIAGG
                     RAGTLAELTADTGVSAALDGLRDGLIEQGLLIRLGLRTALWTSQWLLRAWVLAGFVYFF
                     LINDAYHGLPKFLMVFAPLAALSWAASGLGAGRGATKEGERAVEQARSAAPHLDPAQGA
                     SYEGLGGPAVVLGVALFGTAALMAFDEMFAQTVGLGRYLQLTGVAAVSGGYTGSACSAS
                     AVVCSSGSCGGGDSGGGHHHSCGGGSGGGHHHGCGGSSCGSSCGGGSSCGSSCGGGS"
     gene            5001..5810
                     /locus_tag="ACTIVE_RS40615"
                     /old_locus_tag="ACTIVE_8150"
     CDS             5001..5810
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="ACTIVE_RS40615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8150"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_173099923.1"
                     /sec_met_domain="DUF692 (E-value: 4.8e-74, bitscore: 248.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGVGIGWRPEIAGFVGALPGLRFTEVIAESVHPAHPAPELLAL
                     RERGAAVVPHGVKLSLGGAEPVAAERVAHLAACAEALGSPLVSEHVAFVRAGGIEAGHL
                     LPVPRTRASLDALTANIRRTRAELPVPLAVEPIATLFDWPDAEYTEGDFLTELLERTGA
                     LLLLDVANVYANARNRGEDPVRLLDRLPLERVAYCHVAGGVEQGGRYHDTHTAAVPQEV
                     LDLIAELCARHRPPGFLLERDGHYPAIGEMRAELDAIAAASGLAAIT"
     gene            5860..6321
                     /locus_tag="ACTIVE_RS40620"
                     /old_locus_tag="ACTIVE_8151"
     CDS             5860..6321
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTIVE_RS40620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ACTIVE_8151"
                     /product="hypothetical protein"
                     /protein_id="WP_173099924.1"
                     /transl_table=11
                     /translation="MKEANREAADAGLAAAQEALVRAMTAGGPMPAGFDGAAVRAAAHG
                     ILLKRAGEVARAWPALAAAYGASWKAEFAGWAAERPTGGSFRDGWDFARAHRGGLTGEA
                     ARELALAEISWAYDGASAPRRRRAALRRVPGGVAVLFRGRVRILGGPPR"
     misc_feature    complement(6367..6382)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 25.87"
                     /tool="antismash"
     misc_feature    6417..6437
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.15"
                     /tool="antismash"
     gene            6490..7515
                     /locus_tag="ACTIVE_RS40625"
                     /old_locus_tag="ACTIVE_8152"
     CDS             6490..7515
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /locus_tag="ACTIVE_RS40625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8152"
                     /product="6-phosphofructokinase"
                     /protein_id="WP_173099925.1"
                     /transl_table=11
                     /translation="MRVGVLTGGGDCPGLNAVIRAVVRKGVQVFGYEFVGFRAGWRGPL
                     EGDTVALDVQAVRGILPRGGTILGSSRTNPVKVEGGVERIKDNLAGLGVDALIAIGGED
                     TLGVARELYESGVNVVGVPKTIDNDLNATDYTFGFDTAVNIGMEAVDRLHTTAESHHRA
                     LICEVMGRHAGWIALHVGMAAGANVILIPERPFDIDKVVEYVESRFKTRYAPIIVVSEG
                     AHPMDGQMQLQTGERDAFGHVRLGGIGQALADEIEKRTGKEARATVLGHIQRGGTPTAF
                     DRVLATRFGLQAIDAVRDGDFGKMVALQGTDIVRVGLDEATKELKTVPLERYAEAEVFF
                     G"
     misc_feature    complement(7532..7546)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.1"
                     /tool="antismash"
     misc_feature    7555..7573
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            7620..8852
                     /gene="thiI"
                     /locus_tag="ACTIVE_RS40630"
                     /old_locus_tag="ACTIVE_8153"
     CDS             7620..8852
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853368.1"
                     /locus_tag="ACTIVE_RS40630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8153"
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="WP_173099926.1"
                     /transl_table=11
                     /translation="MTTLETAPADVREQSPVDGEPCVLLKLGEVVLKGKNRELFERRLA
                     DNVRQAVRPIARVDVVRRHGVFIVRKHDADLATMERVAQRITDVMGIVWAHRAWRVGKD
                     LASVERAALELMDGRAGTFAVRSRRRDKRFPMTSTELDRHIGALVADRYGQPVKLKDPV
                     HTLSIEVDRDEVFVYSGGLPGQGGLPVGMSGRGLVLMSGGIDSPVAAYRMMRRGLRVDY
                     LHFSGMPFTGPESIYKAYALVRELDKFQGGSRLFVVPFGKAQQQIKSSGADRLAVIAQR
                     RLMLRTGEVLARRLRGSALITGDALGQVSSQTLANITALDDAVELPILRPLVGMDKIEI
                     MDQARRIRTLSISELPDEDCCTMLAPRRAETRAKIDDLRQIEKRLDVSELADQLAESVQ
                     EHRPIYGDQAS"
     gene            complement(9106..10026)
                     /locus_tag="ACTIVE_RS40635"
                     /old_locus_tag="ACTIVE_8154"
     CDS             complement(9106..10026)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039603.3"
                     /locus_tag="ACTIVE_RS40635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_8154"
                     /product="DUF5954 family protein"
                     /protein_id="WP_173099927.1"
                     /transl_table=11
                     /translation="MTMGDGDAGGRLFPEDLDGVDPVAAVMLADACRSLATYPELVAVG
                     PLFTAAERVAGGWQVVCPPDPLPQGARELLADHLDDRAAGPGEETAHEFRRAAAALRTG
                     PCDAVAAAGRRFAIVRVEQLVRTGPDGPEPPRPSDPDPRPASVPDSYRRPYDLLSGEGT
                     GSDLATAELLCQVLDAAAVSGNEPSGAFLTPVALAPAFTVAERGGGVPGRERRWRPVGR
                     LHATPQRARDSLIGYLRDIAPAVEALAETDADAYAEAAELLADEPRRNGIAVAGRRFRI
                     VRIERITLMGPQGPEPPRPTDFDTP"
     misc_feature    complement(10062..10077)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.28"
                     /tool="antismash"
ORIGIN
        1 cgcagcacga gcgtcacgaa cccgccgatg aacgcggcga ccgccaggaa cgcggcccag
       61 ccgggcacct cccagtccag gatcaccgtg accagcaggt agaccgggcc gccgatcagc
      121 gcgatccagg cgcccttggt gagcgggtcc gcgctcggca gcggcggggg aggcggcggg
      181 atgtagtgcc cctcctcgtc gtcgtccggc gggaactcgg gggcgtcgtc ggccggccgg
      241 atcacccggg cccgcggcag ccggccggtg cggtcgtcgc cctcgcgcgg cggcgccggc
      301 ttctccgcga tgttctcctg ctcgggccac ggcacctcgt cgccctcggg cgcggtgtcg
      361 aagtccgcga cgatctgcgc ccagatcgcg tcctcgtcga ggtcgcggga cggttccgcg
      421 gcgtcgccct tgccgggcgc ggcgccgggc tccccgtcgc cgcggccgct ccccgccgcg
      481 tcgaacacgg cgtcgaacgc gcccttgtcg aacgcggatt cgtcgaacgt cccgtcgagg
      541 ctcccgtcgc gcgggccgtc gtgcaccccg tcgcgcccgg gcggcgggtc ggcggcggcg
      601 tcggcgaggt cggtctccgg acggccgtcg ccctcgccgc gcacgcccgc gtcccgcagc
      661 cgggccaggt gcgcgcgcag gatgccctcc gcggccggct tcatgtcgac gtccacgtac
      721 agccggtcga gcacgtcgcc gaccgggtcg cccgccgtcc cggcgatctc gggcaggccg
      781 tccggcgacg cggcgtaggc cgcaacgccc gcctcgccga gcgccgcgag catcgcgtcg
      841 gccagatgcg gggcgaggtc gaccagcggc gcgtaggtgt cggcggacag tccattgcca
      901 cggcgattca cggggccggc tccttcgacg actccggcgt gaccgactgc accggcatgg
      961 cggcctccgc tcccccattg aggtgtgcgc ccggccgccc gccgcggtgc gcggcggcac
     1021 tcccggacga cccgaaacgt gtgtcccacg gtattcgctg cgccgcgcgg gacaagcccc
     1081 cgggcgcacc cgattgagat ttccgttcag agcctacgtt catccggcgg cggggtgtgc
     1141 cggaccgggc gccgggaccg ggacagtggt gtgggcgggg gacggccggg atgcgaagat
     1201 gtcccatcat taacccccct cggacgggga ctcggtcttg cgcgccgcgt gcacccgcgg
     1261 ctcggaggaa ggtgtcggca tgctctggtt ctggatcctg ctgaggatcg tgctctcccc
     1321 catcatgtac ggcgcgtggt ggccgcggaa ccgggggatg cggaacgtcc cgaagcgcgg
     1381 ccccgcgctg ctggcgtgca accaccagtc gttcgccgac cacttcttcg gcccgctgcc
     1441 gctgatgcgg ccgatcttct tcatcggcaa gggcgagtac ttcaccggcc gggggatcaa
     1501 gggcctgatc agcaaggcgt tcttcaccgg cgtcggggtc gtcccggtgg accgcaccgg
     1561 cggcgccgag gccgacgcgg cgctgcagac cggcctgcgg atcctcggcg agggcaagct
     1621 gctcggcatc taccccgagg gcacccgggc gcccgacgag cggctgtacc gcggcaagac
     1681 gggggtggcg cggctggcgc tgaagtcccg cgtcccggtg atcccgatgg cgatgatcaa
     1741 cacgttccgg ctgatgccgc ccgggcagcc ctacccgcgg ctcggcgtcc ggccgggcgt
     1801 ccggttcggc gagccgatgg acttctcccg ctacttcggc caggaggacg accgcgaggt
     1861 gctccgcacg gtcaccgacg agatcatgaa ggcgatcgag aagctgtccg ggcaggagta
     1921 cgtcgaccgg tacgccgccg aggtgaaggc cgagatggcg ggcaagaccg tccggccggt
     1981 cgaggacggc gacgagggca tcgccgagga ctgatcccgt ggcggacgac accaccgggg
     2041 gcacgacggg cctcgacgcg gcgctgtggc ggtccgtcgc cgtgtaccgc gcgctggcgc
     2101 tggtctacgc cgccgtcgtg atcggcaaga actccggcgg ctacgcgcac ccgctgggcg
     2161 gctgggccgt cctcgcggtg ctgaccgtct ggacgggcgt cgcgacgctg gcgtaccggg
     2221 acccggcccg gcgcgggtgg ccgctcctgg ccgccgacct cgccgtcgcg ttcgggtgca
     2281 tcctcgcgac cgcctgggtg gagacggccc cgcacatcgc ggagggccgc ccgacgctgc
     2341 cggtgtcgtg ggtcgcggcg gccgtgctgg cctgggccgt cgagggcggg cggcggatcg
     2401 gcgtcaccgc cgcgctcgtg ctcggcgtcg cgaacctgct ggtgcacgcc ttcgccgata
     2461 ccgccggcgg gatggggaac accaccctga acggcatcgt gctgctgttc ctcgccggcc
     2521 tggtcgtggg gcaggtggtg cggctcgcgc gggaggcgga ggcgcggctg gcccgggccg
     2581 tcgagctgga ggcggcgacg cgggagcgcg agcggcttgc gcgccgcatc cacgactcgg
     2641 tgctgcaggt cctggcgatg gtgcagcggc gcggcggcga ggccggcggc gaggcggccg
     2701 agctcggccg gctggcgggc gagcaggagg cggcgctgcg cgcgctgatc ggcgccggtc
     2761 ccgcgtccgc cgccgggccc ctgcccgcgg gcgtgcccgc gccgcgcgcc ggcgccggac
     2821 gctccaccga cctgcggccg ctgctgaccg gccaggcgtc cgcggcggtg accgtgtcga
     2881 cccccgcgac gcccgtgccg ctgcccgagc acgacgccca cgagatcgcc gcggccgtcg
     2941 cggccgcgct ggacaacgtc cggcggcact gcggcgacgg cgcccgcgcc tgggtcctgc
     3001 tggaggacgg ccccggcgag gtcaccgtca gcgtccgcga cgacgggccc ggcatgccgc
     3061 ccggacgcct cgatgccgcc gccgcggacg gccggctcgg cgtcgcccag tccatccggg
     3121 gccgcgtccg cgacctcggc ggcacggtct cgatcgtctc cgcccccggc gagggcaccg
     3181 agatcgagat gaccgttccg cggtcgtccg cggcgtttaa ggtgtgatcg tgagcgaggt
     3241 tgcgttgagg gtcatggtcg tggacgacca tccgatgtgg cgcgaggccg tcgcccgcga
     3301 cctcaccgag gccgggcacg aggtcgtcgc caccgcgggg gagggccgca ccgccgtgcg
     3361 catcgccgcc gccgcgcgcc cccgcgtcgc cgtcgtcgac ctccagctgc ccgacatctc
     3421 gggcgtcgag gtcacccggc acctcgccgc ggccgacccg cccgtccggg tcctggtgct
     3481 gtcggccagc ggcgagcagc aggacgtcct ggaggcggtg aaggcgggcg ccaccggcta
     3541 cctgctcaag tccgcgggcc gcgaggagtt cctggacgcg gtccgccgca ccgccgacgg
     3601 cgacgcggtc ttcacccccg gcctcgccgg gctcgtcctc ggcgagtacc ggcggctcgc
     3661 cgccgcgccc gcccgcgagg acgacgacgc gccgcgcctc accgaccgcg agaccgaggt
     3721 gctgcggctc gtcgcgaagg gcctgacgta caagcagatc gcgcagcgcc tcgtcctgtc
     3781 gcaccgcacc gtgcagaacc acgtccagaa caccctcggc aagctccagc tccacaaccg
     3841 cgtcgagctc gtccgctacg ccatcgagaa gggcctggac gaggaggact aggccggttc
     3901 cggccgccac cgccgacaac tcccgcccgg gcgcgtgcgt cagatccgcg acgacgacgc
     3961 tcaaacgacg ggggcacgtt cggtggacga gacgctgtgg ctcctcacgc tgcacttcct
     4021 ggtcgggctc gtgctggtcg cggtcctgtg gcggcggcga tcggcactgt tgcgcggcac
     4081 gccgccgacg cgcgacctgc acccgtacga gatcgcctac ctccagggcg ggggacgcca
     4141 cgcgatcgcc gcatcgctgg cggcgctgcg cctggacggc gcggtcgacg cccacggcga
     4201 cggccggatg gcggcggccc cgccgccgtc cgacgcgccg cgcaccgccg gccatccgct
     4261 ggacggcgcg gtcttcggag cgatcgcggg cggacgcgcc ggcacgctcg ccgagctgac
     4321 cgccgacacg ggcgtgtcgg ccgcgctcga cgggctccgc gacggcctga tcgagcaggg
     4381 cctgctgatc cgcctcggac tccggacggc gctgtggacg agtcagtggc tcctgcgcgc
     4441 ctgggtgctc gccgggttcg tgtacttctt cttgatcaac gacgcctacc acggacttcc
     4501 caagttcctc atggtgttcg cgccgctcgc cgccctgtcc tgggcggcga gcgggctcgg
     4561 ggccggccgc ggggcgacga aggagggcga gcgggccgtc gagcaggcca ggtcggccgc
     4621 cccgcatctc gacccggcgc agggcgcctc ctacgagggc ctgggcggcc cggcggtcgt
     4681 cctcggcgtc gcgctgttcg gcacggccgc gctgatggcg ttcgacgaga tgttcgccca
     4741 gacggtcggg ctcggccgct acctccagct gaccggggtc gcggcggtct cgggcgggta
     4801 cacgggctcg gcctgctcgg cgtccgcggt cgtgtgctcg tccggttcct gcggtggcgg
     4861 cgactccggc ggcggccacc accacagctg cggcggcggt tctggcggcg gccaccacca
     4921 cggctgcggc ggcagcagct gcgggagcag ctgcggaggt ggcagcagtt gcgggagcag
     4981 ctgcggtggc gggagctgac atggacctgg gcgtcgggat cgggtggcgg ccggagatcg
     5041 ccgggttcgt gggggcgctg cccggcctgc gcttcaccga ggtgatcgcg gagtccgtgc
     5101 acccggcgca ccccgctccc gaactcctcg cactgcgcga gcgcggcgcg gcggtcgtcc
     5161 cgcacggggt gaagctgtcg ctgggcggcg cggaaccggt cgccgccgag cgggtcgcgc
     5221 acctggccgc ctgcgcggag gcgctcggct cgccgctggt cagcgagcac gtcgcgttcg
     5281 tccgcgcggg cgggatcgag gccgggcacc tgctgcccgt gccccgcacg cgggcgtcgc
     5341 tggacgcgct caccgccaat atccgccgca cccgggcgga gctgcccgtc ccgctcgccg
     5401 tcgagcccat cgcgacgctc ttcgactggc cggacgccga gtacaccgag ggcgacttcc
     5461 tcaccgaact gctcgaacgc accggcgccc tgctcctgct ggacgtcgcg aacgtgtacg
     5521 ccaacgcccg caaccgcggc gaggaccccg tccggctgct cgaccggctg ccgctggagc
     5581 gcgtcgcgta ctgccatgtc gcgggcggcg tcgagcaggg cggccggtac cacgacacgc
     5641 acaccgccgc cgtcccgcag gaggtcctgg acctgatcgc cgaactgtgc gcgcggcacc
     5701 gtccgcccgg cttcctgctg gaacgcgacg gccactatcc ggcgatcggc gagatgcgtg
     5761 ccgagctgga cgccatcgcc gccgcgtccg gcctggcggc catcacatga gcgaagcgaa
     5821 ccggggggtg tggggggtcg ccccccacag ggtgcaggta tgaaggaagc gaaccgggag
     5881 gcggcggacg cggggctggc ggcggcgcag gaggcgctcg tccgggcgat gacggcgggc
     5941 ggcccgatgc ccgccgggtt cgacggcgcc gcagtgcggg ccgccgcgca cggcatcctg
     6001 ctgaaacggg ccggcgaggt ggcgcgcgcc tggcccgcgc tcgctgccgc gtacggcgcg
     6061 tcctggaagg cggagttcgc cgggtgggcc gccgaacgcc cgacgggggg atctttccgg
     6121 gacggatggg atttcgcgcg ggcgcaccgg ggagggctca ccggggaggc ggcacgggaa
     6181 ctggcgctcg cggagatcag ttgggcctat gacggcgcgt ccgcgccgcg ccgccggagg
     6241 gcggcgctgc ggcgcgtgcc cgggggagtg gcggtgctct tccggggaag ggtccggatc
     6301 ctcgggggcc cgccgcgctg agccggcatc ccgccgaacg gcgtgccgcg ggacatcccc
     6361 gcgggctatg gtctagacca atggcggcac gccggtccgg ccggcgaatg ccctgcgtag
     6421 ccatgccctg acgattagtc tggccatgtg aaccgaggag ggagcccggt ggcgggagaa
     6481 ggagacggca tgcgagtcgg agtgctcacc ggcggtgggg actgccccgg actgaacgcg
     6541 gtcatccgcg cggtcgtccg caagggcgtg caggtcttcg gctacgagtt cgtgggcttc
     6601 cgggccggct ggcgcggtcc cctcgaaggc gacaccgtcg ccctggacgt ccaggccgtg
     6661 cgcgggatcc tgccccgcgg cgggacgatc ctcggctcgt cccggaccaa cccggtcaag
     6721 gtcgagggcg gcgtcgagcg gatcaaggac aacctcgccg gcctcggcgt cgacgccctg
     6781 atcgcgatcg gcggcgagga caccctcggc gtcgcccgcg agctgtacga gagcggcgtc
     6841 aacgtcgtcg gcgtgccgaa gacgatcgac aacgacctca acgccaccga ctacaccttc
     6901 gggttcgaca ccgcggtgaa catcggcatg gaggccgtcg accggctgca caccaccgcc
     6961 gagagccacc accgcgcgct gatctgcgag gtcatgggcc ggcacgcggg ctggatcgcg
     7021 ctgcacgtcg gcatggccgc cggcgccaac gtcatcctca tccccgagcg gcccttcgac
     7081 atcgacaagg tcgtcgagta cgtcgagagc cggttcaaga cccggtacgc gccgatcatc
     7141 gtcgtgtcgg agggcgcgca cccgatggac gggcagatgc agctgcagac cggcgagcgc
     7201 gacgcgttcg ggcacgtccg gctcggcggc atcggccagg ccctcgccga cgagatcgag
     7261 aagcgcaccg gcaaggaggc ccgcgccacc gtgctcgggc acatccagcg cggcggcacc
     7321 cccaccgcgt tcgaccgcgt cctcgccacc cgcttcggcc tccaggccat cgacgccgtc
     7381 cgcgacggcg acttcggcaa gatggtcgcg ctccagggca ccgacatcgt ccgcgtcggc
     7441 ctcgacgagg cgaccaagga gctcaagacc gtcccgctcg agcgctacgc cgaggccgag
     7501 gtcttcttcg gctgaacccg tccctcccgg cgggaacagg tattccgggt cgcgatgttg
     7561 gatccagtgg gccacactgg aggccgcccc ttgacctgtt cccgccggga ggaccacgca
     7621 tgaccacgct cgagaccgcg cccgcggacg tccgggagca gtccccggtc gacggggagc
     7681 cgtgcgtgct gctcaaactc ggcgaggtcg tcctcaaggg caagaaccgc gagctgttcg
     7741 agcgccgcct cgccgacaac gtccggcagg ccgtccgccc catcgcccgc gtcgacgtcg
     7801 tccgccggca cggcgtcttc atcgtccgca agcacgacgc cgacctcgcc accatggagc
     7861 gcgtcgcgca gcggatcacc gacgtcatgg gcatcgtgtg ggcgcaccgc gcctggcggg
     7921 tcggcaagga cctcgccagc gtcgagcgcg ccgccctcga gctcatggac ggccgcgccg
     7981 gcacgttcgc cgtccggtcc cggcgccgcg acaagcggtt cccgatgacc tccacggagc
     8041 tcgaccggca catcggcgcc ctcgtcgccg accgctacgg ccagcccgtc aagctcaagg
     8101 accccgtcca caccctgtcg atcgaggtcg accgcgacga ggtgttcgtc tactccggcg
     8161 gcctgcccgg ccagggcggc ctgcccgtcg ggatgagcgg ccgcggcctc gtgctgatgt
     8221 ccggcggcat cgactcgccc gtcgccgcct accggatgat gcgccgcggc ctgcgcgtcg
     8281 actacctgca cttctccggc atgccgttca ccggccccga gtcgatctac aaggcgtacg
     8341 cgctggtccg cgagctggac aagttccagg gcgggtcccg gctgttcgtc gtcccgttcg
     8401 gcaaggccca gcagcagatc aagtcgtcgg gcgccgaccg gctcgccgtg atcgcgcagc
     8461 gccggctcat gctgcgcacc ggcgaggtcc tcgcccgccg cctgcgcggc tccgcgctga
     8521 tcaccggcga cgcgctcggc caggtctcca gccagaccct cgccaacatc accgccctcg
     8581 acgacgccgt cgagctgccc atcctgcgcc cgctcgtcgg catggacaag atcgagatca
     8641 tggaccaggc gcgccgcatc cggaccctgt ccatctccga gctgcccgac gaggactgct
     8701 gcacgatgct cgcgccgcgc cgcgccgaga cccgcgccaa gatcgacgac ctccgccaga
     8761 tcgagaagcg cctcgacgtc tccgaactcg ccgaccagct cgccgagtcc gtccaggaac
     8821 accgccccat ctacggcgac caggcgagtt gatttcaccg ccctcggcgg tcgggccttg
     8881 gaggccttcc cttcaacggg cggtgcggcg atcgctgcat cgctccggct cgcccagggg
     8941 ctcgctgcgc gatcgggttc tcgcaggctc gaacccggct tcgcacgcga tcgcaacgct
     9001 gaggtcgtcg gcggctgggc ctacgacgcg cgccccttgc ggtcggcgcg gggttgcggt
     9061 gttgttgcgc ctgcgcagct tgcgcccgtc ggcgcggttg gcgggtcagg gggtatcgaa
     9121 gtcggtgggg cggggcggtt cggggccctg ggggcccatg agggtgatgc gctcgatgcg
     9181 gacgatccgg aagcggcggc cggcgacggc gatgccgttg cggcggggct cgtccgccag
     9241 cagctcggcg gcctccgcgt aggcgtcggc gtcggtctcg gcgagggcct ccacggcggg
     9301 tgcgatgtcg cgcaggtagc cgatcagcga gtcgcgggcc cgctgcgggg tggcgtgcag
     9361 gcggccgacg gggcgccagc ggcgctcgcg gcccggcacg ccgccgccgc gctcggcgac
     9421 ggtgaacgcg ggcgccagcg cgacgggcgt gaggaacgcg ccggacggct cgttcccgct
     9481 gaccgccgcc gcgtccagca cctggcacag cagctcggcg gtggcgaggt cggacccggt
     9541 gccctcgccg gacagcaggt cgtaggggcg gcggtaggag tccgggacgg atgccgggcg
     9601 cgggtcgggg tcgctcgggc ggggcggctc ggggccgtcc gggccggtcc gcaccagctg
     9661 ctcgacccgc acgatcgcga accggcggcc ggccgccgcc accgcgtcgc acgggccggt
     9721 ccgcagcgcc gccgcggccc gccggaactc gtgcgcggtc tcctcgccgg gcccggcggc
     9781 gcggtcgtcg agatggtcgg cgagcagctc gcgggcgccc tgcggcagcg ggtccggcgg
     9841 gcagacgacc tgccagccgc cggcgacccg ctccgcggcg gtgaacagcg gcccgaccgc
     9901 caccagctcg gggtaggtgg cgagggagcg gcaggcgtcc gccagcatca cggcggcgac
     9961 cgggtcgacg ccgtccaggt cctccgggaa cagccgtccc ccggcgtcgc catcgcccat
    10021 cgtcatgccg gacatcatcc tcgccgcccc ccgatctggc gagtggttca gaccaattcg
    10081 ttaccgctcc ggccgcgtct cccgggcttc cccggacggc ggacgggcgc gacgagcacg
    10141 acgaggctcg cggcgagcag cgccaggccc gcgtaggcgg ccccgccgag gtgctcgccc
    10201 agcaccgccg tgccgaggac ggcggcgacg gccggctcgg cgagcgtcag cgtcgtcgcg
    10261 gtggtggcgg gggtgccgcg caggccccgc gcgaacagca cgtagccgag gccggtggtg
    10321 accgcgccga ggtacagcgc ggtcgccgcg ccggacccgg tgagcagcca tccggcgggc
    10381 tcggccgcga ggaccgggac gagcagcacc gcggcggcgc cgaacagcgc cccggcggtc
    10441 gcccggtcgt cctcgccgcg ggtgatcagg acggacgcgg tggtcgcgta caccgcgtag
    10501 gacagcccgg acagcagcgc gagcgcgatc ccggcggggt cgacgccgga gccgtgcccg
    10561 gcgccgacga gcagggcgca gcccgcgacg gcgcccgcgg tcgcggccgt ccagcggccg
    10621 gtgaggacgg cccgccgggt cgccagcccg atcagcccgg tgaacgcggg cgcgctgccg
    10681 atcgtgacga cggtgccgac ggccacgccc gtccgggaga cggcgacgaa gaacgcgacc
    10741 tggtagaccg cgatcgcgac ggcgccgagg gcgagcggcg cccgggaggc ccggccgcgc
    10801 aggaggtgga
//
