LOCUS       NZ_CP053892            22786 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Actinomadura verrucosospora strain NRRL:B18236 chromosome.
ACCESSION   NZ_CP053892
VERSION     NZ_CP053892.1
KEYWORDS    RefSeq.
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M.
  TITLE     Actinomadura verrucosospora NRRL-B18236 (PFL_A860) Genome sequencing
            and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10214634)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2016
            Assembly Method                   :: Newbler v. 3.0
            Assembly Name                     :: A860_AS2.mpe1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013211575.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,277
            CDSs (total)                      :: 9,181
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 96
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 18 of 71
            Pseudo Genes (incomplete)         :: 57 of 71
            Pseudo Genes (internal stop)      :: 2 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:15:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3581882
            Orig. end                         :: 3604668
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Peter Leadlay,
            NRRL-B18236 (patent collection, not in online catalogue).
FEATURES             Location/Qualifiers
     region          1..22786
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="12"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22786
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22786
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12786](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12786
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            complement(132..1739)
                     /locus_tag="ACTIVE_RS15170"
                     /old_locus_tag="ACTIVE_3026"
     CDS             complement(132..1739)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /locus_tag="ACTIVE_RS15170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3026"
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="WP_173095669.1"
                     /transl_table=11
                     /translation="MTLPPRYAWSIPGWQPERPRNDPAVGEIWCYTDRFGYDPGDDVAV
                     HVHTTADVYDVEVIRDGAAPQTYFRRTGLPGAAHPTPDDAYAAGCGWPVAFTVPVGTDW
                     PSGFYLVVVGIDDEHGRRHEREHFFVVRARAGERSSAALVLTTSTMLAYNDWGGANAYR
                     GLGDDPYADVPTPRQSALRPIARGMLRKPEGAARNRHTGVPPIGWEPRHPAYEWAYANG
                     YSRHHADALWATYERPFVVWAERNGYRLDYLTQHDLHYRPDLLDGYESLVIVGHDEYWT
                     AEMRDAADAFVDGGGNLARFGANYVWQVRFEDGGATQVCHKVPGADPVAASDPSRDTTA
                     WDAPGIGRPGAATMGLTGLGGAYARYGSTTPRSSGGYTVYRPDHWAFEGTDLYYGDVFG
                     GDACIVSFEVDGVDYTFRKGLPYPTHEDGAPENLEILAMAPAVVGEIDRWGGRVPLGAP
                     EGEAMGLVDALYDGNPPEFRRDARYGAAMIGVFERGGTVFNAGTCEWVSGLIARDPFTE
                     RITHNVLRRLAPGSREAR"
     gene            complement(1736..3199)
                     /locus_tag="ACTIVE_RS15175"
                     /old_locus_tag="ACTIVE_3027"
     CDS             complement(1736..3199)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /locus_tag="ACTIVE_RS15175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3027"
                     /product="APC family permease"
                     /protein_id="WP_173095670.1"
                     /transl_table=11
                     /translation="MTVAPPGPDAPSTGLRSGAVGTRDLVFFVVAAAAPLTVMAGFGPL
                     AFVYGGAATPSGYLLAGLIYLLFTVGFAAMSRHVSGSGAFYAYITRGLGRAPGAGAAVL
                     AYAAYALGQIGFAAAAGLFASTTIEDLLGVHVPWAVAAVALSLLVGAMAYRRVDIGAKV
                     MAVLLTGEIGVLLVLAAAVLVRGGHEGLSAGSFAPDRVFTSGLGAMLVIPLVVYIGFEQ
                     TAIYSEETRDPRRTVARATYIAVGFLAVFYTFISWTLYMAIGPNALAAALSGDPSTLVY
                     RLDDAYVGGAMTKVMEVLMVTSYLAGVLALQNACGRYLFAQGREGLLPAALRRVGASTG
                     TPSVACVLQTAIVVAALAGFGLSGLDPYRQVVVWTNTPTLVGVLALQIMTSAAVVGYLG
                     RRRAGESPWQRLIAPGASVLVLSAVLVLLLTKLPVLTGMGALGNTLIMLPLPVAFAAGC
                     VRALWLRSARPGVYARVGSTDPFAPERTT"
     gene            complement(3311..4849)
                     /locus_tag="ACTIVE_RS15180"
                     /old_locus_tag="ACTIVE_3028"
     CDS             complement(3311..4849)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /locus_tag="ACTIVE_RS15180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3028"
                     /product="PucR family transcriptional regulator"
                     /protein_id="WP_173095671.1"
                     /transl_table=11
                     /translation="MTDSGLTVHDMAADAGLRLAVVAGRAGLGRRIRSAHVAELPDPTP
                     WLRGGELLMTTGMRAGEAGGPAAFLRRLADAGVAAVALGLGSTLAYRRAPAAWTEPAEE
                     LGLPLLEVPEETPFIAVTDAVYRRLAELRYGDVEHALRAQRVLTGAAVRPGGVLAIARG
                     LAAEAGLWVVVTDRAGRPLAAVPQDAAGRLGALGADLARVVRKGPHASARRTDGGDDVL
                     FQPLGAGRLRGVLVCGADAELGAFQRMLIGSAVSLMSIELEHREALAGAERRRRAGLVR
                     ELVAGRHAPDAAAGVLASLGLGGGRVRVVVVPDVPDAAARLESFAEAFPGAAAAADASG
                     LVAIMPEEDGLAKRLAAELPGLAAGMGGPVGPDGLELSLRQARQVLALERRSGPGVYDA
                     LARPNFQMLLGLGPPAVLRAYADAILGPIDRLPKGRGDELIRTLWAWIEADCSYERAAD
                     RLGIHRHTLRERLQRVERLTGKRLAARELGDVWMALAAREAADGAAAPAPEPGP"
     gene            complement(4911..5447)
                     /locus_tag="ACTIVE_RS15185"
                     /old_locus_tag="ACTIVE_3029"
     CDS             complement(4911..5447)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633642.1"
                     /locus_tag="ACTIVE_RS15185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3029"
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_173095672.1"
                     /transl_table=11
                     /translation="MSTRALIVVDMLNPYGHQDAGLLTASVERIIEPLRRLLERARERG
                     DVRLLYVNDNHGDFTADRRDLVRRALDGERPDLVEPIVPPDDCDFLAKVRHSAFYGTPL
                     EYLLSREGVEEIILAGQVTEQCVLYSALDAYVRHFKIKVVRDAVAHIHPDLGYAALRMM
                     DLNMRAKIVPAAQCL"
     gene            5621..7489
                     /locus_tag="ACTIVE_RS15190"
                     /old_locus_tag="ACTIVE_3030"
     CDS             5621..7489
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568945.1"
                     /locus_tag="ACTIVE_RS15190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3030"
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="WP_173095673.1"
                     /transl_table=11
                     /translation="MDEPQGPAPRWCSCRVPDEPAGGHEGHRHGLSRRQALGGLTAAGA
                     LALAGWPGSAEAAAPGKRSGRDVTITVMGTSDIHSHALDWDYYKDAAYSDSAGNVVGLA
                     RVSSLVQQIRRRRGRDHTLLFDAGDTIQGTPLGFYYATVEPITKTGQTHPMATQMNAIG
                     FDAVALGNHEFNYGLPFLNKWISQMDAPVLAANAVHAGTDRPAFRPYVIKRMHVPGHPP
                     IRVGLLGLTNPGVAIWDKANVSGKLDFLDLVETAKKWVPIVRAQGVDVMVVSAHAGDSG
                     LSSYTGDIPVENASAMVARQVPGIDAILFGHAHIDVPQRFVTNETTGESVVMSEPKCWG
                     ERLSVFDLTLRRERGRWKVVGKSATTVNTNTVEEDPALVRLVKKQHDAVVAYVNQVVAT
                     STEEMSAAESCWKDTAILDYVNAVQTAKVTEAVAGTEYASLPVVSIAAPFSRAATFPAG
                     KVTIKDIAGLYIYDNTLLGSVLTGAQIKDYLEYSARYYKQVAAGDPVDPASWTNAHDLP
                     DYNYDQFSGVTYDIDIAQAEGSRIRNLAFNGTTVADDQKFVVAVNNYRQSGGGGFPHIT
                     TAPVVYNAQLAIREAIVAYASAAGTIDPKSFHVENWRLVRDGTPVF"
     gene            7525..8166
                     /gene="mftR"
                     /locus_tag="ACTIVE_RS15195"
                     /old_locus_tag="ACTIVE_3031"
     CDS             7525..8166
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852715.1"
                     /locus_tag="ACTIVE_RS15195"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process.; MftR, the
                     mycofactocin system transcriptional regulator, is an
                     uncharacterized TetR family DNA-binding transcription
                     factor. Its role is inferred by context. It occurs as part
                     of the biosynthesis locus for mycofactocin, a partially
                     characterized electron carrier derived from the terminal
                     Val-Tyr dipeptide of the precursor peptide MftA, through a
                     radical SAM enzyme-mediated process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ACTIVE_3031"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_246342853.1"
                     /transl_table=11
                     /translation="MTSEGGTAGRQRAMGRPRATSPAVLERLAFELFARKGFDATTVDD
                     IAAAAGIARRTFFRYFSSKNDLVWGDFEGQLRRLRALLDEADPHAPMMDAVRRAVVEFN
                     RFDPQDVPWHRQRMQLILGVPTLQADATLRYASWRSVITAFVAGRTGAPASALVPRLAG
                     NVVLSACVSAYEFWLASGEDTVLADVLDEALRQVSAGLSAVPDGAGSVSA"
     gene            complement(8194..9726)
                     /gene="mftG"
                     /locus_tag="ACTIVE_RS15200"
                     /old_locus_tag="ACTIVE_3032"
     CDS             complement(8194..9726)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene="mftG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852714.1"
                     /locus_tag="ACTIVE_RS15200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3032"
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="WP_216858430.1"
                     /transl_table=11
                     /translation="MSILSPRRPDVLVVGAGGSGAVLAARLSEDPGCEVVLAEAGPVPS
                     ALPDFPGGLLDARLVPGAQPGNPAVRTFPAHLAPGLPYAAARGRFLGGSTTVNGGYFIR
                     ARREDFDRWSVHGDLWSYDRVLPLMRALETDLDFGAAALHGGDGPVPVRRTGLSHPAAA
                     AFQAAARSLGHPDDPDKNAQAAPGFGPVPSNTVDGQRINTGIAYLLGEAARRPNLTILG
                     DCAVRSVVVGRGRATGVVLERGGRRTVLDAGQVILCAGSLSSPHLLHLSGIGPADDLAA
                     HGIPVAHDLPAVGAALSDHPQVTVEWTPQRPLPAPSGTWLGGALHLPSSGGAAAGDLEI
                     LQSLVPVMGLVGGTASVPGAPLPFLVSVLTPRRGGTLRTVSADPDVPPRITYGYLETED
                     DRRRMREAVRAAAAVTEALGEVSGGPAEPGPRPLDDDGLLDAWILARLGTSQHTCGTVP
                     MGGAVDGHGRVHGLTGLRVADTSILPDAPLRGPAATAVLIGEVIARAVRAGG"
     misc_feature    complement(9755..9771)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.03"
                     /tool="antismash"
     gene            9871..9990
                     /gene="mftA"
                     /locus_tag="ACTIVE_RS46640"
                     /old_locus_tag="ACTIVE_3033"
     CDS             9871..9990
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049342.1"
                     /locus_tag="ACTIVE_RS46640"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3033"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_216858431.1"
                     /transl_table=11
                     /translation="METNAPQNEAAEQDDRAQDIDVAESLIEEVSIDGMCGVY"
     misc_feature    9873..9884
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            10001..10288
                     /gene="mftB"
                     /locus_tag="ACTIVE_RS15210"
                     /old_locus_tag="ACTIVE_3034"
     CDS             10001..10288
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852712.1"
                     /locus_tag="ACTIVE_RS15210"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA.; MftB, a small
                     protein, is a peptide chaperone that assists the radical
                     SAM enzyme MftC in performing two modifications to the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     peptide, MftA. MftB's role is analogous to the role of PqqD
                     in the biosynthesis of PQQ, a cofactor that derives
                     entirely from a Tyr and a Glu in the precursor PqqA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3034"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_246342855.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.1e-29,
                     bitscore: 101.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQPVPGLDLDRAWDLHPQVSVRPEPFGALLYHFGTRKLSFLKDR
                     RLLEVVRSLGEAPTAREACSRAGVPAADLPRFGAALGALAGSSMLVERTP"
     aSDomain        10031..10270
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ACTIVE_RS15210_Mycofactocin_RRE.1"
                     /evalue="3.30E-32"
                     /identifier="RREFam016.1"
                     /label="ACTIVE_RS15210"
                     /locus_tag="ACTIVE_RS15210"
                     /protein_end="90"
                     /protein_start="10"
                     /score="101.6"
                     /tool="antismash"
                     /translation="DRAWDLHPQVSVRPEPFGALLYHFGTRKLSFLKDRRLLEVVRSLG
                     EAPTAREACSRAGVPAADLPRFGAALGALAGSSML"
     gene            10285..11541
                     /gene="mftC"
                     /locus_tag="ACTIVE_RS15215"
                     /old_locus_tag="ACTIVE_3035"
     CDS             10285..11541
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852711.1"
                     /locus_tag="ACTIVE_RS15215"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="ACTIVE_3035"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_173095674.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.9e-37, bitscore:
                     128.2, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.2e-26, bitscore: 93.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6.3e-08, bitscore: 31.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 4e-13, bitscore: 48.6,
                     seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTNRPAPQNPAQRSGTRLVDLFEYGLDAPICLTWELTYACNL
                     SCAHCLSSSGRRDPRELSTEECKAVIDELEAMQVFYVNIGGGEPTVRPDFWELLDYATA
                     HHVGVKFSTNGVRITPEVAERLAANDYVDVQISLDGATPEVNDAVRGPGSYDTAIRAMR
                     NLRDAGMKNFKLSVVCTRHNIPQMDAFKAIADEYGAQLRLTRLRPSGRGADVWDELHPR
                     QEQQRELYDWLVAHGDSVLTGDSFFHLSAYGKALPGLNLCGAGRVVCLIDPVGDVFACP
                     FAIHDEFLAGNIRQPGGFARVWRESDLFRNLREPQTGGACASCGFYDTCKGGCMAAKFF
                     TGLPLDGPDPECVQGHGETLLAQRPEDRAGIPKPSADHSHRTSPPRRRPVPVTLMTSRP
                     ERPPVSACEENPLAGLRLT"
     gene            11596..12786
                     /gene="mftD"
                     /locus_tag="ACTIVE_RS15220"
                     /old_locus_tag="ACTIVE_3036"
     CDS             11596..12786
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852710.1"
                     /locus_tag="ACTIVE_RS15220"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="ACTIVE_3036"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_173095675.1"
                     /sec_met_domain="TIGR03966 (E-value: 1.1e-206, bitscore:
                     685.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKNPWFETVAEAQRRAKRRLPHMVYGALVAGSERGRTIDANTKA
                     FGDLGFAPRVVGHKAERDLSTTVMGVETSMPVLISPTGVQAVHPDGEVAVARAAANRGV
                     IMGLSSFASKPVEEVAEANPNVFFQMYWSGDRDAMVQRLDRAKKAGAKALIATLDWSFS
                     NGRDWGSPMIPEKIDFKTAAKLAPGVLPHPGWLWKFAKEGRIPDLTTPNLKPPNGPAPT
                     FFGAYGEWMQTPPPTWEDVAWLREEWGGPFLLKGVTRVDDAKRAVDAGVTALSVSNHGG
                     NNLDTTPATIRVLPSIAEAVGDQIEVLLDGGVRRGGDVAKALALGARAVLIGRAYLWGL
                     AANGQAGVENVLDIMRGGLDSAVLGLGHSSVGELNPDDLVIPPGFALTLGGGGSAG"
     gene            12788..13525
                     /gene="mftE"
                     /locus_tag="ACTIVE_RS15225"
                     /old_locus_tag="ACTIVE_3037"
     CDS             12788..13525
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /locus_tag="ACTIVE_RS15225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3037"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_246342857.1"
                     /transl_table=11
                     /translation="MAGAGDLAGAAWTDVRAAPLVLVPVGSCEQHGPHLPLSTDTVIAE
                     AVAARAADALRPDAGGQVLVAPPIAYGASGEHAGFPGTVSIGHEALASVLVETVRSLAL
                     WAGRVVFVNGHGGNVPTLDAAAGRMRVEGHDTAWVGCGVPGGDAHAGFTETSVMLHLAP
                     GLVRPFDAVTGDARPLAAIMPELVAGGVRAVSPSGVLGDPAGASAERGREITAAMVASV
                     VRRIGGGRADERGRLLDPAAPRA"
     gene            13522..15027
                     /gene="mftF"
                     /locus_tag="ACTIVE_RS15230"
                     /old_locus_tag="ACTIVE_3038"
     CDS             13522..15027
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="ACTIVE_RS15230"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3038"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_173095676.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.1e-21,
                     bitscore: 76.5, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPKDRFRLPDGLAVALARDTSLWSGGRVATGGAPWKVVRLADA
                     AGPHLAALRRGGRAGLADGSPVGRALARRLLDLGMADPLPAPRPGPHDVTVVIPAYERA
                     DELERSLAAVGGDVPVIVVDDASPDPGPLRRVADAYGARLVRHARNRGPAAARNTGLRL
                     VETSFAAFVDSDCRPMDGWLDVLVPHFDDPRVAAVAPRIVPLSEDTGLLARYETARSAL
                     DMGVRPALVRPGGRLGFVPTATLLVRVAAVPEDPFDEDLRLGEDVDFVWRLGDRGWNVR
                     YEPAARVAHTPRLHPAAWAKRRHEYGTSAADLARRHPGRLAPARPSVWNLAVLALLARG
                     RPGAAAGVAAVTAALLARRLSRLPADWSLAAAIVGKGVAADAAAIGHALRREWWPLGLL
                     ALAASRRSRVARAAAAAMLAPIALEWARERPAIDPVGYTVLRLAEDVAYGSGVTAASAR
                     ARSAAPLLPDVRLPESFPSVRDRARKAARRWIAAVRPAGRDPR"
     gene            complement(15030..15851)
                     /locus_tag="ACTIVE_RS15235"
                     /old_locus_tag="ACTIVE_3039"
     CDS             complement(15030..15851)
                     /NRPS_PKS="Domain: PKS_KR (12-186). E-value: 3.8e-13.
                     Score: 41.7. Matches aSDomain:
                     nrpspksdomains_ACTIVE_RS15235_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852403.1"
                     /locus_tag="ACTIVE_RS15235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3039"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_173095677.1"
                     /sec_met_domain="adh_short (E-value: 3.2e-32, bitscore:
                     111.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARSTGMRLTGKVAFITGAARGQGRAEAVRLASEGADVIAVDLVG
                     PASTYVPYEPAAQSDLDETAKLVEAQGRRVVSRKADVRDLAALQSAVQEGVAELGRLDV
                     VVANAGIVNYGRTWELEEEQWQDVIDTNLTGVWKTVKATVPTLIEQGQGGSIIITSSVA
                     GLKGLPFLAHYASSKHGVVGLAKVLANELGEHDIRVNTVHPHGVRTGMTAGTMTELLEQ
                     SPMLGPIYMPTLPYEMVEPEDIANIVAFLASDEGKYITGAQLPVDLGALNR"
     aSDomain        complement(15294..15815)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTIVE_RS15235_PKS_KR.1"
                     /evalue="3.80E-13"
                     /label="ACTIVE_RS15235_PKS_KR.1"
                     /locus_tag="ACTIVE_RS15235"
                     /protein_end="186"
                     /protein_start="12"
                     /score="41.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAFITGAARGQGRAEAVRLASEGADVIAVDLVGPASTYVPYEPAA
                     QSDLDETAKLVEAQGRRVVSRKADVRDLAALQSAVQEGVAELGRLDVVVANAGIVNYGR
                     TWELEEEQWQDVIDTNLTGVWKTVKATVPTLIEQGQGGSIIITSSVAGLKGLPFLAHYA
                     SSKHGVVGLAK"
     misc_feature    complement(15806..15820)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.32"
                     /tool="antismash"
     misc_feature    15825..15838
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            16452..18761
                     /locus_tag="ACTIVE_RS15240"
                     /old_locus_tag="ACTIVE_3041"
     CDS             16452..18761
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630554.1"
                     /locus_tag="ACTIVE_RS15240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3041"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_246342859.1"
                     /transl_table=11
                     /translation="MALNVTLERQTGRLPAEFTSFVGRETELDQVGRLIDGHRLVTLTG
                     PGGVGKTRLAVRTAARARDAFDGGVYFVALSGLTEPSLLAQAVCDALGLAEQGGEPAAS
                     RLADHLADRRVLLVLDTCEHLLDECAALAEALLQSAPGLRVLVTSRQPLDVLGEQTIAV
                     APLGRPDPGRAGAPGEECDSVKLFTERAAAAVQGWTLHHGNREAVVRLCDRLDGIPLAI
                     ELAAVQLRTFSADQVLSRLGSRLFQARGMRTSVPRHQTLRAAIDWSHALCSPGERLLWA
                     RLSVFAGDFELDAAEHVCSADDLPAEHVVELLTGLVDKSIVQRADRGGQVRYRMLDSLR
                     EYGVERLAAQGGEALLRRRCFDWYTGRLRTARAELAGAGQAGWLAWFGCERANIRALLG
                     HVMATAETATIRDAMEALGRLWMLHGMAGECRHWIDRLLEVRDTTGEGWSGFLGVAALT
                     MTLQDDLAAAGALLDRALVQARAEGDADTAGMVVACQGVAALFAGDVDLALQLLGAADE
                     VGAGVAGDDVLFLLVEVFRGVAYLFKGDYPAAVAHAGQGARTGEEHGELWVRSYGMLVC
                     GLALWLAGDLAASIERLRRSLRIKRDLGDELGMTLGLEAVAGCLCSGGEYVRAVRLLGA
                     ADRMRESTQTAWFGPHHAMLREIHTGRAAEALGERRYRAAFEEGGRLAPAQAIRDALGE
                     APAPDGAEDAAGACPLTDRELQVAALVAEGLSNRDIAERLTIAKRTADSHVEHILAKLG
                     FSSRAQIAVWITEQSP"
     misc_feature    16464..16476
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    18811..18826
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.65"
                     /tool="antismash"
     gene            18940..20367
                     /locus_tag="ACTIVE_RS15245"
                     /old_locus_tag="ACTIVE_3042"
     CDS             18940..20367
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655712.1"
                     /locus_tag="ACTIVE_RS15245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3042"
                     /product="sugar porter family MFS transporter"
                     /protein_id="WP_173095678.1"
                     /transl_table=11
                     /translation="MQGFSSATVYEGPLAEVPPDGLRKIWLWAVLIAVGGFLFGFDTGV
                     ISGALLYIKTDFGLNSFEQSSVVSVLVLGAMVGAMGSGRIADRIGRRRILGLEGLVFLV
                     GTVIAVLSTGYAMLVLGRLVLGLAVGGASATVPVYLSEISPKEIRGRTLTLNQLLITIG
                     ILVAYLTNLAFSGAGDWRAMIGIGAVPAAVIVAASLWVLPESAAWLASRGDRAEARRLV
                     ASVTNEATADRLLDRYDRDRAREGDDGGAAGWRTLLAADVRPALVVGLALAAVQQFGGI
                     NTIIYYAPTIIEETGLTASNSIVYSIAIGIINLVMTLIAIRVIDRSGRRPLLLFSLGAM
                     TVMMALLGLSFVADWSSGLSLVCMVLYIAGYAVGTGPVFWTLIGEIFPPRARAAGSSAS
                     TAVNWLANFVVSLIFLPIANVIGQGETFWIFAVIAAAGWVFVSRFVPETKERDFTQVDA
                     DLRSRFGRPAPASADPD"
     gene            complement(20387..20968)
                     /locus_tag="ACTIVE_RS15250"
                     /old_locus_tag="ACTIVE_3043"
     CDS             complement(20387..20968)
                     /EC_number="3.5.1.28"
                     /GO_function="GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA]"
                     /GO_process="GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /locus_tag="ACTIVE_RS15250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3043"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_173095679.1"
                     /transl_table=11
                     /translation="MTAPAGPPVDDRLVSWWGSLKRRAPESITTVVLHATEIPTLDEAW
                     DYAERSADGTEDGTGVCGHLYVDRDGGCHRFVPLDRVAAHAAGHNTPSIGIELVNTGRH
                     PDHFHSRSQEPDEDFPPDQTAALIGLLTVLKETHPALAHLVRHSDLDQETVPASDDPDV
                     PVRRRIDPGPRFPWDDVRRRWERMPGPFPA"
     misc_feature    21026..21039
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.42"
                     /tool="antismash"
     misc_feature    complement(21027..21040)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.4"
                     /tool="antismash"
     misc_feature    21113..21128
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.03"
                     /tool="antismash"
     gene            21118..21636
                     /locus_tag="ACTIVE_RS15255"
                     /old_locus_tag="ACTIVE_3044"
     CDS             21118..21636
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653895.1"
                     /locus_tag="ACTIVE_RS15255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3044"
                     /product="DUF1269 domain-containing protein"
                     /protein_id="WP_173095680.1"
                     /transl_table=11
                     /translation="MSDLIAVAYPDVDTAVTVRDRLIDMQRRKLITLGDAAVVEKRPDG
                     KIKLHQIHSTTRSGALWGGVWGGLLGMLFFAPLVGAAVGAAAGAAGGSMTDVGVDDSFM
                     RDVGEKLRPGGAVLFLLVISVTPDRVIPQIAEYGGEVMQTSLSNEAEANLREALKAAQH
                     AQPAGASPH"
     gene            complement(21684..21944)
                     /locus_tag="ACTIVE_RS15260"
                     /old_locus_tag="ACTIVE_3045"
     CDS             complement(21684..21944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911759.1"
                     /locus_tag="ACTIVE_RS15260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ACTIVE_3045"
                     /product="hypothetical protein"
                     /protein_id="WP_173095681.1"
                     /transl_table=11
                     /translation="MREPDVEIGASVEADEVVFRETPDVEERAGADPEGESGARTKREN
                     LPRPAEVNVTYRDVRVSHRLSARLAEPELAEPDEPDDDGGE"
ORIGIN
        1 ggctcgtcgc ccgcggcgcc gcccgcgtcg gccgccacga gcccgcgccc ccgctcggcg
       61 ccggccacat cgtgatgacc gaccccgagg gcaacgagtt ctgcctggac tgaccgcccg
      121 ctggcccaag gtcagcgggc ctcccgggat cctggcgcga ggcggcgcag gacgttgtgg
      181 gtgatccgct cggtgaacgg gtcgcgggcg atgagcccgc tgacccattc gcaggttccg
      241 gcgttgaaga cggtgcctcc gcgctcgaag acgccgatca tcgccgcgcc gtaccgggca
      301 tcgcgccgga actcgggcgg gtttccgtcg tagagggcgt cgaccaggcc catggcctcg
      361 ccttcgggcg cgccgagcgg gacccggccg ccccagcggt cgatctcgcc gacgacggcg
      421 ggcgccatgg ccaggatctc caggttctcc ggagcgccgt cctcgtgcgt cgggtacggc
      481 agtcccttgc ggaaggtgta gtcgacaccg tccacctcga acgacacgat gcacgcgtcg
      541 ccgccgaaca cgtccccgta gtagaggtcg gtgccctcga aggcccagtg gtccgggcgg
      601 tagacggtgt aaccaccgct ggagcgcggc gtggtcgagc cgtagcgggc gtaggcgccg
      661 ccgaggccgg tcaggcccat cgtcgcggcg cccgggcggc cgatgcccgg cgcgtcccag
      721 gcggtggtgt cgcgggacgg gtcggacgcg gcgaccgggt ccgcgccggg gaccttgtgg
      781 cagacctggg tggcgccgcc gtcctcgaac cggacctgcc agacgtagtt ggcgccgaac
      841 cgggcgaggt tgccgccgcc gtcgacgaac gcgtcggcgg cgtcgcgcat ctcggccgtc
      901 cagtactcgt cgtggccgac gatgacgagg ctctcgtagc cgtcgaggag gtcggggcgg
      961 tagtgcaggt cgtgctgggt gaggtagtcc aggcggtagc cgttgcgctc cgcccagacc
     1021 acgaacggcc gctcgtaggt ggcccagagc gcgtcggcgt ggtggcggga gtagccgttg
     1081 gcgtaggccc attcgtaggc ggggtggcgc ggctcccagc cgatcggcgg gacgccggtg
     1141 tgccggttcc gggcggcccc ttcgggcttg cggagcattc cccgcgcgat cggccgcagc
     1201 gcggactgcc ggggcgtcgg gacgtcggcg tacgggtcgt caccaagtcc gcggtaggcg
     1261 ttggcgccgc cccagtcgtt gtaggcgagc atcgtgctgg tggtgaggac cagggcggcg
     1321 ctggaacgct cccccgccct cgcccggacg acgaagaagt gctcgcgctc gtgccgccgc
     1381 ccgtgctcgt cgtcgatgcc gacgacgacg aggtagaacc cggacggcca gtccgtcccc
     1441 accggcaccg tgaacgcgac gggccagccg cagccggcgg cgtacgcgtc gtccggcgtg
     1501 gggtgcgccg cgccgggcag gcccgtccgg cggaagtacg tctgcggtgc ggcgccgtcc
     1561 ctgatcacct cgacgtcgta gacgtcggcg gtggtgtgga cgtggaccgc cacgtcgtcc
     1621 cccgggtcgt agccgaaccg gtcggtgtag caccagatct cgcccacggc gggatcattg
     1681 cggggccgct cgggctgcca gccggggatg gaccaggcgt agcggggcgg gagtgtcatg
     1741 tggtcctctc aggggcgaac gggtcggtgg agccgacgcg ggcgtagacg ccgggccggg
     1801 cggaccgcag ccacagcgcc cggacgcagc cggcggcgaa cgcgaccggc agcgggagca
     1861 tgatcagggt gttgccgagg gcgcccatgc cggtgagcac cggcagcttc gtcagcagca
     1921 ggaccaggac ggcgctcagc accagcacgg acgcgcccgg cgcgatcagc cgctgccacg
     1981 gcgactcgcc cgcgcggcgg cggccgaggt accccacgac cgcggccgat gtcatgatct
     2041 gcagcgccag gacaccgacg agcgtcggcg tgttcgtcca caccacgacc tgccggtagg
     2101 ggtcgaggcc ggacagcccg aatccggcga gcgccgcgac gacgatcgcc gtctgcagca
     2161 cgcaggcgac gctcggcgtc ccggtcgacg cgccgacccg gcgcagcgcg gcgggcagca
     2221 gcccctcgcg gccctgcgcg aacaggtacc ggccgcacgc gttctgcagg gccagcacgc
     2281 cggcgaggta gctggtcacc atcagcacct ccatgacctt ggtcatggcg ccgccgacgt
     2341 aggcgtcgtc gagccggtac accagcgtgg acgggtcccc cgacagcgcc gcggcgagcg
     2401 cgttcgggcc gatcgccatg tagagcgtcc agctgatgaa ggtgtagaag accgcgagga
     2461 acccgaccgc gatgtaggtg gcgcgggcga cggtgcggcg cgggtcgcgg gtctcctcgc
     2521 tgtagatggc ggtctgctcg aacccgatgt agacgaccag cgggatgacg agcatcgcgc
     2581 cgaggccgga ggtgaacacc cggtccggcg cgaaactccc ggccgacagc ccctcgtggc
     2641 cgccccggac gaggacggcg gcggcgagca cgagcaggac cccgatctcc cccgtgagca
     2701 gcaccgccat gaccttcgcg ccgatgtcga cccgccggta cgccatggcg ccgaccagca
     2761 gcgacagcgc gaccgccgcg accgcccacg ggacgtgcac gccgaggagg tcctcgatcg
     2821 tcgtggacgc gaacaggccc gccgcggcgg cgaagccgat ctgcccgagg gcgtacgcgg
     2881 cgtaggcgag cacggcggcg ccggcgccgg gcgcccggcc gagcccccgg gtgatgtagg
     2941 cgtagaaggc gccgctgccg gacacgtgcc ggctcatcgc ggcgaacccg accgtgaaca
     3001 gcaggtagat caggcccgcg agcaggtagc cggacggggt ggccgcgccg ccgtacacga
     3061 acgcgagcgg cccgaacccg gccatcacgg tgagcggcgc ggcggccgcg acgacgaaga
     3121 acaccaggtc ccgggtgccg acggcgccgg atctgagtcc ggtggacggg gcgtccggac
     3181 cgggcggagc gactgtcatg ggggtctcct tcggcgagtt ggagacacgc taaagccgca
     3241 ggtcggcgcc gtgcccctgc cgatcgggca ggtccggcgg gccgttttcg cccgatcgga
     3301 gggtcgcccg ctagggcccc ggctccggcg cgggggcggc ggcgccgtcg gccgcctcgc
     3361 gggccgcgag cgccatccac acgtcgccca gctcccgcgc ggccagccgc ttgccggtga
     3421 ggcgctccac ccgctggagg cgttcgcgca gcgtgtgccg gtggatgccg agccggtcgg
     3481 cggcgcgctc gtaggagcag tccgcctcga tccaggccca cagcgtcctg atcagctcgt
     3541 cgccgcggcc cttggggagc cggtcgatcg ggccgaggat cgcgtcggcg tacgcgcgca
     3601 gcaccgcggg cggtccgagg ccgagcagca tctggaagtt cgggcgcgcg agcgcgtcgt
     3661 agacgccggg gccgctgcgg cgctcgagcg ccagcacctg ccgggcctgc cggaggctca
     3721 gctccaggcc gtccggtccg accgggccgc ccatcccggc cgcgagcccc ggcagctccg
     3781 ccgcgaggcg cttcgccagc ccgtcctcct ccggcatgat cgcgacgagt ccggacgcgt
     3841 cggcggcggc cgcggcgccg gggaacgcct ccgcgaacga ctccaggcgg gccgccgcgt
     3901 ccggcacgtc ggggacgacg accacgcgga cgcggccgcc gccgagcccc agcgacgcca
     3961 gcacccccgc cgccgcgtcc ggggcgtgcc gtcccgccac cagctcgcgg accagccccg
     4021 cgcggcgcct gcgctcggcg ccggcgagcg cctcccggtg ctccagctcg atcgacatca
     4081 gcgagaccgc cgagccgatc agcatccgct ggaacgcgcc gagctccgcg tccgccccgc
     4141 agaccagcac gccgcgcagc cgcccggcgc cgagcggctg gaagaggacg tcgtcaccgc
     4201 cgtcggtccg gcgggccgac gcgtgcggcc ccttgcggac gacccgggcg aggtcggctc
     4261 cgagggcgcc gagccgtccg gccgcgtcct gcgggacggc ggcgaggggg cgtccggccc
     4321 ggtcggtgac gaccacccac aggcccgcct cggcggcgag gccgcgcgcg atcgccagga
     4381 cgccgccggg ccggacggcc gcgccggtca gcacgcgctg cgcccgcagg gcgtgctcga
     4441 cgtcgccgta gcgcagctcg gccagccgcc ggtacaccgc gtcggtgacc gcgatgaacg
     4501 gcgtctcctc cgggacctcc agcagcggca gcccgagctc ctcggccggc tccgtccacg
     4561 cggcgggcgc gcgccggtac gccagcgtcg agccgagccc gagcgccacg gcggcgaccc
     4621 cggcgtccgc gagccgccgc aggaacgccg cggggccgcc cgcctcgccg gcgcgcatgc
     4681 cggtcgtcat gagcagttcc ccgccgcgca gccagggcgt cgggtcgggg agctcggcga
     4741 cgtgcgcgga ccggatccgg cggccgagcc ccgcgcggcc ggccaccacc gccagccgca
     4801 gcccggcgtc ggccgccatg tcgtgcacgg tcagcccgga atcggtcatt ctccatgcgt
     4861 agcagacggc ccgccgcgac cgctcccgcg cctcacgggc actgtgcggt tcagaggcac
     4921 tgggcggcgg ggacgatctt ggcgcgcatg ttgaggtcca tcatgcggag cgccgcgtag
     4981 ccgaggtcgg ggtggatgtg ggcgaccgcg tcccgcacca ccttgatctt gaagtgccgg
     5041 acgtaggcgt ccagcgcgct gtagaggacg cactgctcgg tgacctggcc ggcgaggatg
     5101 atctcctcga cgccctcgcg ggagagcagg tactccagcg gcgtgccgta gaaggcgctg
     5161 tgccggacct tggccaggaa gtcgcagtcg tccggcggga cgatcggctc gaccaggtcg
     5221 gggcgctcgc cgtccagggc gcggcggacc aggtcccggc ggtcggcggt gaagtcgccg
     5281 tggttgtcgt tgacgtacag caggcgcacg tcgccgcgct cgcgggcgcg ctccagcagc
     5341 cgccgcagcg gttcgatgat ccgctccacg ctcgcggtga gcaggcccgc gtcctggtgg
     5401 ccgtaggggt tcagcatgtc gacgacgatc agcgctctgg tgctcacggc cggtgcctgc
     5461 ccggcctgcg ccgtcgcaca cctgggccgg gcggcgcggc gcaaggacgg ggtctgccaa
     5521 ggttgggccg ccaggatcgg ggattctcac cggctcccgg ccggcgcggc gggcttatgg
     5581 tgacgggtcg tctcccttgg caccctcgga aggacgtgtg atggacgaac cgcagggccc
     5641 ggcgccgcgc tggtgttcgt gtcgagtgcc ggacgagccg gccggcggcc atgaggggca
     5701 ccggcacggc ctcagccgcc ggcaggcgct gggcgggctg accgccgccg gcgcgctggc
     5761 cctcgcggga tggcccgggt cggcggaggc cgcggcgccc gggaagcggt ccggacgcga
     5821 cgtgacgatc accgtcatgg gcacgtccga catccacagc cacgccctgg actgggacta
     5881 ctacaaggac gcggcgtaca gcgacagcgc ggggaacgtg gtcgggctgg cgcgggtgtc
     5941 gagcctggtg cagcagatcc gccggcggcg agggcgcgat cacacgctgc tcttcgacgc
     6001 cggggacacc atccagggca ccccgctcgg cttctactac gcgaccgtgg agcccatcac
     6061 caagacgggt cagacgcacc cgatggcgac gcagatgaac gccatcggat tcgacgcggt
     6121 ggcgctcggc aatcacgagt tcaactacgg gctgccgttc ctgaacaaat ggatcagcca
     6181 gatggacgcg ccggtgctgg ccgcgaacgc ggtgcacgcg gggacggacc gtccggcgtt
     6241 ccgcccgtac gtcatcaagc ggatgcacgt gccggggcat ccgccgatca gggtcgggct
     6301 gctcggcctg acgaatccgg gcgtcgcgat ctgggacaag gcgaacgtgt ccgggaagct
     6361 cgatttcctg gacctggtcg agacggccaa gaaatgggtc ccgatcgtgc gcgcgcaggg
     6421 cgtcgacgtc atggtcgtca gcgcgcacgc gggcgacagc gggctgtcgt cctacaccgg
     6481 cgacattccg gtggagaacg cgtcggcgat ggtcgcccgg caggtcccgg gaatcgacgc
     6541 gatcctgttc gggcacgcgc acatcgacgt cccccagcgg ttcgtcacga acgagaccac
     6601 cggcgagtcg gtggtgatga gcgagccgaa gtgctggggc gagcggctgt cggtgttcga
     6661 cctcacgctg cggcgcgagc ggggccggtg gaaggtcgtc gggaagtccg cgacgacggt
     6721 gaacacgaac accgtggagg aggacccggc gctcgtccgg ctggtgaaga agcagcacga
     6781 cgcggtggtc gcctacgtga accaggtcgt ggcgacgtcc accgaggaga tgtcggcggc
     6841 ggagtcgtgc tggaaggaca ccgcgatcct cgactacgtc aacgcggtgc agaccgcgaa
     6901 ggtcaccgag gccgtggcgg ggaccgagta cgcgtccctt ccggtggtgt cgatcgcggc
     6961 gccgttcagc cgcgccgcga cctttcccgc cggaaaggtc accatcaagg acatcgccgg
     7021 tctctacatt tacgacaaca ccctgctcgg cagcgtcctg acgggcgcgc agatcaagga
     7081 ctacctcgaa tactcggcgc ggtactacaa gcaggtcgcc gccggcgatc ccgtcgatcc
     7141 cgcgtcgtgg acgaacgccc acgaccttcc cgactacaac tacgaccagt tctccggggt
     7201 gacgtacgac atcgacatcg cccaggcgga agggtcgcgg atccgcaatc tcgccttcaa
     7261 cgggacgacg gtcgcggacg accagaagtt cgtcgtcgcg gtgaacaact accggcagtc
     7321 cggcggcggg ggcttcccgc acatcacgac ggccccggtc gtctacaacg cccagctcgc
     7381 gatccgggag gcgatcgtcg cgtacgcgtc cgcggccggg acgatcgacc cgaagtcgtt
     7441 ccacgtggag aactggaggc tggtccgcga cggcacgccg gtcttctagg tgcggacccg
     7501 tgcgggccgt gggaccctgg tggcgtgacc tcggaaggcg ggacggccgg ccgccagcgg
     7561 gccatgggca ggccgcgcgc gacctcgccg gccgtcctgg agcggctggc gttcgagctg
     7621 ttcgcgcgca aggggttcga cgcgacgacc gtcgacgaca tcgcggcggc ggcggggatc
     7681 gcgcggcgca cgttcttccg ctacttctcc tcgaagaacg acctggtgtg gggcgacttc
     7741 gaggggcagt tgcggaggct gcgcgcgctg ctcgacgagg cggacccgca cgccccgatg
     7801 atggacgcgg tgcgccgtgc cgtggtcgag ttcaaccggt tcgacccgca ggacgtgccg
     7861 tggcaccggc agcgcatgca gctgatcctc ggggtgccga cgctgcaggc ggacgcgacg
     7921 ctgcggtacg cgtcctggcg gtcggtgatc accgcgttcg tcgccgggcg caccggcgcg
     7981 ccggcgtcgg cgctggtgcc gcggctggcg ggcaacgtcg tgctgtccgc ctgcgtctcc
     8041 gcgtacgagt tctggctggc gtccggtgag gacacggtgc tggccgacgt gctggacgag
     8101 gcgctgcggc aggtgagcgc gggcctgtcg gcggtgccgg acggcgccgg ctcggtgtcg
     8161 gcgtaacgcg gtgccggcct agcggacgcg gggtcagccg ccggcgcgga cggcgcgggc
     8221 gatcacctcg ccgatgagga cggccgtcgc ggcgggcccg cgcagcggcg cgtccggcag
     8281 gatcgaggtg tcggcgacgc gcagcccggt caggccgtgc acgcggccgt ggccgtcgac
     8341 ggcgccgccc atcggcacgg ttccgcaggt gtgctgggac gtgccgagcc gggcgaggat
     8401 ccacgcgtcg agcaggccgt cgtcgtccag cggccggggc ccgggctcgg cgggaccgcc
     8461 ggagacctcg ccgagcgcct cggtgacggc ggccgccgcc cgcaccgcct cgcgcatccg
     8521 gcgccggtcg tcctcggtct ccaggtagcc gtaggtgatc cgcggcggga cgtccgggtc
     8581 ggcggagacg gtgcggagcg tcccgccgcg ccggggcgtc agcaccgaca cgaggaaggg
     8641 cagcggcgcg ccggggaccg acgccgtccc gccgaccagg cccatgaccg gcacgaggga
     8701 ctggaggatc tccaggtcgc ccgccgcggc gccgccggac gagggcaggt ggagggcgcc
     8761 gccgagccag gtgccggacg gcgcgggcag cgggcgttgc ggcgtccatt ccacggtgac
     8821 ctgggggtgg tcgctgagcg cggcgcccac ggcgggcagg tcgtgcgcga cggggatgcc
     8881 gtgggcggcg aggtcgtcgg cggggccgat gccggacagg tgcagcaggt gcggtgagga
     8941 gagcgagccc gcgcacagga tgacctgccc cgcgtccagc accgtccgcc gcccgccgcg
     9001 ttcgagcacc accccggtgg ccctgccgcg gccgacgacg accgaccgca ccgcgcagtc
     9061 gccgaggatg gtgaggttgg gccggcgggc cgcctcgccg agcaggtagg cgatcccggt
     9121 gttgatccgc tggccgtcca cggtgttcga cgggaccggg ccgaagccgg gcgcggcctg
     9181 ggcgttcttg tcggggtcgt ccgggtgccc gagggagcgg gcggcggcct ggaacgcggc
     9241 ggcggcggga tggctcaggc cggtgcggcg gaccgggacc gggccgtcgc cgccgtgcag
     9301 cgcggcggcg ccgaagtcga ggtcggtctc cagcgcgcgc atgagcggca ggacccggtc
     9361 gtaggaccac aggtcgccgt ggacggacca gcggtcgaag tcctcgcgcc gcgccctgat
     9421 gaagtagccg ccgttgacgg tggtggaccc gccgaggaac cgtccgcgcg ccgccgcgta
     9481 gggcaggccg ggcgccaggt gggccgggaa cgtccggacg gcgggattcc caggctgcgc
     9541 gccggggacg agccgcgcgt ccagcagccc gccggggaag tccggaagcg cggagggcac
     9601 cggcccggcc tcggccagaa ccacctcgca gccggggtcc tcgctgagcc gggccgccag
     9661 gaccgcgccg ctgccgcccg cccccaccac gagcacgtcg gggcggcgcg gggacaggat
     9721 gctcacccgg gtttccggcg gatcatggag cggaaccttc ctttatgaca ctcgatgcat
     9781 ttagtatccg tccaggaccg atggtacgag ccgccccgca cgccggagcg gccgggccgt
     9841 cgcacgccgc acccgcatga gggaggaccc atggagacga acgctccgca gaacgaggcc
     9901 gccgagcagg acgaccgcgc ccaggacatc gacgtcgccg agtcgctgat cgaggaggtc
     9961 tcgatcgacg gcatgtgcgg cgtctactag gccgcacgcc gtgacgcagc ccgtgccggg
    10021 cctcgacctc gaccgcgcct gggacctgca cccgcaggtc tcggtgcggc ccgagccctt
    10081 cggcgcgctc ctctaccact tcgggacgcg caagctgtcg ttcctgaagg accgccgact
    10141 cctggaggtc gtgcggtccc tcggggaggc gcccaccgcc agggaggcgt gcagccgggc
    10201 cggcgtgccc gcggcggacc tgccgaggtt cggcgcggcg ctcggcgccc tcgccggctc
    10261 ctccatgctc gtggaaagga ccccatgacc accacgacga accgacccgc tccgcagaac
    10321 cccgcgcagc ggtccgggac gcggctggtc gacctgttcg agtacgggct cgacgcgccg
    10381 atctgcctga cctgggagct cacctacgcc tgcaacctgt cgtgcgcgca ctgcctgtcc
    10441 agctcgggac ggcgcgaccc gcgcgagctg agcaccgagg agtgcaaggc cgtcatcgac
    10501 gagctggagg ccatgcaggt cttctacgtc aacatcggcg gcggcgagcc gacggtccgg
    10561 cccgacttct gggagctgct ggactacgcc accgcgcacc acgtgggcgt gaagttctcc
    10621 accaacggcg tccggatcac cccggaggtc gccgagcggc tcgccgccaa cgactacgtg
    10681 gacgtgcaga tctcgctgga cggcgcgacg cccgaggtca acgacgccgt ccgcggcccc
    10741 ggctcctacg acaccgcgat ccgcgcgatg cggaacctgc gcgacgccgg catgaagaac
    10801 ttcaagctgt cggtggtgtg cacgcggcac aacatcccgc agatggacgc gttcaaggcg
    10861 atcgccgacg agtacggcgc gcagctgcgg ctgacgcggc tgcgcccgtc cggccgcggc
    10921 gcggacgtgt gggacgagct gcacccgcgc caggagcagc agcgggagct gtacgactgg
    10981 ctggtcgcgc acggcgacag cgtgctgacc ggcgactcgt tcttccacct gtccgcctac
    11041 ggcaaggcgc tgcccggcct gaacctgtgc ggcgcggggc gcgtcgtctg cctgatcgac
    11101 ccggtcggcg acgtgttcgc ctgcccgttc gcgatccacg acgagttcct cgcgggcaac
    11161 atccggcagc cgggcgggtt cgcccgcgtg tggcgcgagt cggacctgtt ccggaacctg
    11221 cgcgagccgc agaccggcgg ggcgtgcgcg tcctgcgggt tctacgacac gtgcaagggc
    11281 ggctgcatgg ccgcgaagtt cttcaccggc ctgccgctgg acggccccga ccccgagtgc
    11341 gtgcaggggc acggcgagac gctgctcgcg cagcgcccgg aggaccgggc ggggatcccg
    11401 aagccgtccg ccgaccactc gcaccgcacc tcgccgccgc gccgccgccc ggtgccggtc
    11461 acgctgatga cgagccgccc ggagcgtccg ccggtgagcg cctgcgagga gaacccgctg
    11521 gccggtctgc ggctgaccta gcggttccgc cggcggcccc gcagcggtcc gcccacccat
    11581 cctggagaca tcgagatgtt caagaacccg tggttcgaga ccgtggccga ggcgcagcgc
    11641 cgggccaagc ggcggctgcc gcacatggtc tacggggcgc tggtcgcggg ctcggagcgc
    11701 ggccggacga tcgacgccaa caccaaggcg ttcggcgacc tcggcttcgc gccgcgcgtc
    11761 gtcgggcaca aggcggagcg cgatctgtcc accaccgtga tgggcgtgga gacctccatg
    11821 ccggtgctca tctcccccac cggcgtgcag gccgtgcacc ccgacgggga ggtcgcagtg
    11881 gcgcgggccg ccgcgaaccg gggcgtcatc atggggctca gctcgttcgc gagcaagccc
    11941 gtcgaggagg tcgcggaggc caacccgaac gtcttcttcc agatgtactg gagcggcgac
    12001 cgggacgcga tggtccagcg cctcgaccgc gcgaagaagg cgggcgcgaa ggcgctgatc
    12061 gccacgctcg actggtcgtt ctccaacggc agggactggg gcagccccat gatcccggag
    12121 aagatcgact tcaagacggc ggcgaagctc gcgcccggcg tgctgccgca tccgggctgg
    12181 ctgtggaagt tcgccaagga gggccggatc ccggacctca ccaccccgaa cctcaagccg
    12241 ccgaacggcc cggcgccgac gttcttcggc gcgtacggcg agtggatgca gacgccgcct
    12301 cccacctggg aggacgtcgc gtggctgcgc gaggagtggg gcggcccgtt cctgctcaag
    12361 ggcgtcaccc gcgtcgacga cgcgaagcgc gccgtggacg ccggggtgac cgcgctgtcg
    12421 gtgtccaacc acggcggcaa caacctcgac acgacgcccg ccacgatccg ggtgctgccg
    12481 tcgatcgccg aggcggtcgg cgaccagatc gaggtgctgc tggacggcgg cgtgcggcgc
    12541 ggcggcgacg tcgccaaggc cctcgcgctc ggcgcgcgcg ccgtgctgat cggccgcgcc
    12601 tacctgtggg gcctggccgc gaacgggcag gcgggggtgg agaacgtcct cgacatcatg
    12661 cgcggtggcc tcgactccgc ggtgctcggg ctcggccact cctccgtcgg cgaactgaac
    12721 cccgacgatc tggtcatccc gcccggcttc gcgctgacgc tcggcggcgg cggttctgcg
    12781 ggctgacgtg gccggtgccg gagacctggc gggggccgcc tggacggacg tccgggcggc
    12841 cccgctcgtc ctggtgccgg tcgggtcgtg cgagcagcac gggccgcacc tgccgctgtc
    12901 caccgacacg gtgatcgccg aggccgtcgc cgcgcgcgcg gcggacgccc tgcggccgga
    12961 cgccggtggg caggtgctcg tcgcgccgcc gatcgcgtac ggggcgagcg gcgagcacgc
    13021 cggcttcccc ggcacggtct cgatcgggca cgaggcgctg gcctcggtgc tcgtggagac
    13081 cgtgcggtcg ctcgccctgt gggcggggcg cgtcgtcttc gtcaacgggc acggcggcaa
    13141 cgtccccacc ctcgacgccg ccgccggccg gatgcgggtc gaggggcacg acaccgcctg
    13201 ggtcggctgc ggcgtgcccg gcggcgacgc gcacgccggg ttcaccgaga cgtccgtgat
    13261 gctgcatctg gcccccggcc tggtaaggcc gttcgacgcg gtcaccgggg acgcccgccc
    13321 gctcgccgcg atcatgccgg agctggtcgc gggcggcgtg cgcgccgtct ctccgtccgg
    13381 cgtgctcggc gacccggccg gggcgtccgc cgagcgggga cgcgagatca cggcggccat
    13441 ggtggcgtcg gtcgtccgcc ggatcggcgg cgggcgcgcg gacgagcgcg gacgcctcct
    13501 cgacccggcg gctccgcggg catgagcgcg cccaaggacc ggttccggct gcccgacggc
    13561 ctcgccgtcg cgctggcccg cgacacctcg ctctggtcgg gcgggcgcgt ggccaccggc
    13621 ggcgcgccct ggaaggtggt gcggctggcg gacgcggcgg gcccgcacct ggcggcgctg
    13681 cgccgggggg gccgggcggg cctcgccgac ggctcgccgg tcgggcgggc gctggcccgc
    13741 aggctcctcg acctcggcat ggccgacccg ctcccggcgc cgcgtcccgg cccgcacgac
    13801 gtaacggtcg tgatccccgc ctatgagcgc gccgacgagc tggaacggtc gctggcggcc
    13861 gtcggcggcg acgtgccggt gatcgtcgtg gacgacgcgt cgcccgaccc cgggccgctg
    13921 cggcgggtcg cggacgcgta cggcgcccgg ctcgtccggc acgcccgcaa ccgggggccc
    13981 gccgcggcgc gcaacaccgg cctgcgcctg gtggagacat cgttcgccgc gttcgtggac
    14041 tccgactgcc gcccgatgga cggctggctc gacgtcctcg tcccgcactt cgacgacccg
    14101 cgcgtcgccg ccgtcgcccc ccggatcgtc ccgctgtcgg aggacaccgg gctgctggcg
    14161 cgctacgaga ccgcgcgctc ggcgctcgac atgggggtgc ggcccgcgct ggtccgtccg
    14221 ggcggccggc tcgggttcgt accgaccgcg acgctgctcg tgcgggtcgc ggccgtgccg
    14281 gaggacccgt tcgacgagga cctgcggctc ggcgaggacg tcgacttcgt ctggcggctc
    14341 ggcgaccggg ggtggaacgt ccgctacgag cccgccgcgc gggtcgcgca cacgccgcgg
    14401 ctgcacccgg cggcctgggc gaagcggcgg cacgagtacg gcacgtccgc cgccgacctg
    14461 gcgcggcggc atcccggcag gctcgcgccg gcccgcccgt cggtgtggaa cctcgccgtg
    14521 ctcgcgctgc tggcgcgggg acgtcccggc gcggccgcgg gcgtcgcggc cgtgacggcg
    14581 gcgctgctgg ccaggcggct gtcgcggctg cccgccgact ggagcctggc cgccgcgatc
    14641 gtcggcaagg gcgtcgccgc cgacgccgcc gcgatcgggc acgcgctgcg ccgcgagtgg
    14701 tggccgctcg gcctgctcgc gctcgccgcc tcgcgccgca gccgggtcgc ccgcgcggcg
    14761 gccgcggcca tgctcgcccc gatcgcgctg gaatgggcgc gggagcggcc cgccatcgac
    14821 cccgtcggct acaccgtcct gcggctcgcc gaggacgtcg cctacggatc gggggtgacg
    14881 gcggcgtccg cccgcgcgcg ctcggccgct cccctgctcc cggacgtccg actccccgag
    14941 tcgttcccgt ccgtccgcga ccgggcgcgc aaggccgcca ggcggtggat cgcggccgtc
    15001 cgcccggccg gccgcgatcc gcgctgagat cagcggttca gcgcaccgag gtccacgggc
    15061 agctgggcac cggtgatgta cttgccctcg tcggaggcca ggaacgcgac gatgttcgcg
    15121 atgtcctccg gctcgaccat ctcgtagggc agcgtcggca tgtagatggg cccgagcatc
    15181 ggggactgct ccaggagctc ggtcatcgtc ccggcggtca tgccggtccg caccccgtgc
    15241 gggtggacgg tgttgacccg gatgtcgtgc tcgcccagct cgttcgcgag caccttcgcc
    15301 agtcccacga cgccgtgctt ggacgaggcg tagtgcgcga ggaacggcag gcccttgagc
    15361 ccggccaccg agctggtgat gatgatcgag ccgccctggc cctgctcgat gagcgtcggg
    15421 accgtggcct tgaccgtctt ccacaccccc gtgaggttgg tgtcgatgac gtcctgccac
    15481 tgctcctctt cgagctccca ggtgcggccg tagttcacga tcccggcgtt ggccaccacg
    15541 acgtccagcc ggccgagctc ggcgacgccc tcctggacgg ccgactggag cgcggcgagg
    15601 tcgcgcacgt ccgccttgcg gctcaccacg cggcgcccct gcgcctccac cagcttggcg
    15661 gtctcgtcca ggtcggactg agcggcgggc tcgtagggga cgtaggtgct ggccggcccg
    15721 acgaggtcga ccgcgatgac gtcggcgccc tcggacgcca gccggacggc ctccgcgcgc
    15781 ccctgcccgc gcgccgcgcc ggtgatgaaa gcgaccttcc ctgtcaaacg catgcctgtc
    15841 gaacgcgcca tggcttcttc ctccacatgg gtcagacccg gcgacgcagg ggtggaaggc
    15901 catttcgggc ggacaacggc tgcgctgcca tcgggccgat aagcggtcca cgccatagtg
    15961 gcactgagtg ccaaaagggg caagaggtca cttgtgccac ggcgcggtca ccccgcgtca
    16021 ccagcgggga cggtgctcgc gcgcgccctg gagcccgcca ccgccaccgg cgccgacagc
    16081 acggtgacgg tggcgggggt cagggtcccg gcggccggag gttccccccg gatccccggc
    16141 cgccgggccg gcggaccgcg cgggaacgcc ggtggggcgg cccgccgggg gcatcgccgt
    16201 gcgacctcgc gcaccctgtc tcgcgcggtg cgtcgtcctc gcccgtccga acggacggcc
    16261 cgctccccat caccggaacg gcccgtgccc cctgcggccg gtgcccatcc tggcggagcg
    16321 cccggcgtcc gccatcggta gatgaaccga tcttccctac ctattcgagg tcgccttccg
    16381 tagcgtcccg actacaatcc aagagtccct ctggtcgccg gaagcccggc gggaactgac
    16441 tcggagacgg cgtggcgctg aatgtgacgc tggaacggca gacgggaaga ctccccgcgg
    16501 aattcaccag cttcgtcgga cgggagacgg agctcgacca ggtcgggcgg ctgatcgacg
    16561 ggcaccgcct ggtcacgctg accgggcccg gcggggtcgg caagaccagg ctcgcggtcc
    16621 gcaccgcggc ccgcgcgcgc gacgccttcg acggcggcgt ctacttcgtc gcgctgtccg
    16681 gcctcaccga gccgtcgctg ctggcgcagg ccgtgtgcga cgcgctcggg ctcgccgagc
    16741 agggcggcga gcccgccgcg tcccggctgg ccgaccacct cgccgaccgg cgggtgctgc
    16801 tcgtcctgga cacctgcgag cacctgctcg acgagtgcgc cgcgctggcc gaggcgctgc
    16861 tgcagagcgc gcccgggctg cgcgtgctgg tgaccagccg ccagccgctc gacgtgctgg
    16921 gcgagcagac catcgcggtc gcgccgctcg gccgcccgga ccccggccgc gccggcgcgc
    16981 ccggcgagga gtgcgactcg gtgaagctgt tcaccgagcg cgccgccgcg gccgtccagg
    17041 gctggacgct ccaccacggc aaccgcgagg ccgtcgtccg gctctgcgac cggctcgacg
    17101 ggatccccct cgcgatcgag ctcgccgcgg tgcagctgcg cacgttctcc gccgaccagg
    17161 tgctcagccg gctcggcagc cggctgttcc aggcccgcgg gatgcgcacg agcgtcccgc
    17221 ggcaccagac gctgcgcgcc gcgatcgact ggagccacgc gctctgctcg cccggcgagc
    17281 ggctgctgtg ggcgcggctg tcggtgttcg ccggcgactt cgagctcgac gccgccgagc
    17341 acgtctgctc cgccgacgac ctgcccgccg agcacgtcgt cgagctgctc accgggctgg
    17401 tggacaagtc gatcgtgcag cgcgccgacc gcggcggcca ggtccggtac cggatgctcg
    17461 acagcctgcg cgagtacggc gtcgagcggc tcgccgcgca gggcggggag gcgctgctgc
    17521 ggcggcgctg cttcgactgg tacaccgggc ggctgcggac cgcccgcgcc gagctcgcgg
    17581 gcgccgggca ggccgggtgg ctcgcctggt tcggctgcga gcgcgccaac atccgcgcgc
    17641 tgctcggcca cgtcatggcg accgcggaga ccgcgacgat ccgggacgcg atggaggcgc
    17701 tcggccggct gtggatgctg cacggcatgg cgggcgagtg ccggcactgg atcgaccggc
    17761 tgctggaggt ccgcgacacc accggggagg gctggtcggg gttcctcggc gtcgcggcgc
    17821 tgacgatgac gctccaggac gacctcgccg ccgccggggc gctgctcgac cgggcgctgg
    17881 tgcaggcgcg ggcggagggc gacgcggaca ccgccggcat ggtcgtcgcg tgccagggcg
    17941 tcgcggcgct gttcgcgggc gacgtcgacc tcgcgctgca actgctcggc gccgccgacg
    18001 aggtgggcgc gggcgtcgcc ggcgacgacg tgctgttcct gctcgtggag gtgttccgcg
    18061 gcgtggcgta cctgttcaag ggcgactacc cggcggccgt cgcgcacgcc gggcagggcg
    18121 cgcggacggg cgaggagcac ggcgagctgt gggtgcgctc ctacggcatg ctcgtgtgcg
    18181 ggctcgcgct gtggctcgcg ggcgacctgg ccgcgtcgat cgagcggctc cgccggtcgc
    18241 tgcggatcaa gcgcgacctc ggcgacgagc tcggcatgac gctcgggctg gaggccgtcg
    18301 ccgggtgcct gtgcagcggc ggcgagtacg tccgcgccgt ccgcctcctc ggcgccgccg
    18361 accggatgcg cgagtccacc cagaccgcgt ggttcggccc gcaccacgcg atgctccggg
    18421 agatccacac cggccgggcc gcggaggccc tcggcgagcg gcgctaccgc gcggcgttcg
    18481 aggagggcgg gcggctcgcg cccgcgcagg cgatccggga cgcgctcggc gaggcccccg
    18541 ccccggacgg cgccgaggac gcggccggcg cgtgcccgct caccgaccgg gagctgcagg
    18601 tcgcggcgct ggtggcggag ggcctgtcca accgcgacat cgccgagcgg ctgacgatcg
    18661 ccaagcgcac cgccgactcg cacgtcgagc acatcctggc caagctcggc ttctcctccc
    18721 gcgcccagat cgccgtctgg atcaccgagc agtccccctg accggcgccg ggagccgggc
    18781 gccggcggag ccccggagcc ggacggtgcg tgtgtccacg atcacggagg gtagggcggg
    18841 gtcagcggtg ccggcgccga tcggggaggg cacggaggat gacgacgttc cgaacggccg
    18901 cgcgcggcgg gccgggcgcg gccgggcggc gggcctgacg tgcaggggtt ctccagcgcg
    18961 acggtctacg aggggccgct ggccgaggtg ccgccggacg ggctgcgcaa gatctggctc
    19021 tgggccgtcc tgatcgcggt cggcgggttc ctcttcgggt tcgacaccgg ggtgatctcc
    19081 ggggccctgc tgtacatcaa gaccgacttc gggctgaact cgttcgagca gtccagcgtg
    19141 gtcagcgtgc tggtgctggg cgcgatggtc ggggcgatgg ggtcggggcg gatcgccgac
    19201 cggatcgggc gccggcggat cctcggcctg gaggggctcg tcttcctggt cggcacggtg
    19261 atcgccgtgc tgtccaccgg gtacgcgatg ctggtgctcg ggcggctcgt cctcggcctc
    19321 gcggtcggcg gcgcgtcggc gaccgtgccg gtgtacctct ccgagatctc ccccaaggag
    19381 atccggggcc gcacgctgac cctcaaccag ctgctgatca cgatcggcat cctcgtcgcc
    19441 tacctgacca acctcgcgtt ctccggggcc ggcgactggc gggccatgat cgggatcggc
    19501 gcggtgcccg ccgcggtgat cgtcgcggcg tcgctgtggg tgctgccgga gtcggcggcc
    19561 tggctggcct cgcgcgggga ccgggccgag gcgcgccggc tggtcgcctc cgtcaccaac
    19621 gaggccaccg ccgaccggct cctcgaccgc tacgaccgcg accgggcgcg ggagggcgac
    19681 gacggcggcg cggccggctg gcggacgctg ctcgccgccg acgtgcggcc cgcgctggtc
    19741 gtcgggctgg cgctcgcggc cgtgcagcag ttcggcggca tcaacacgat catctactac
    19801 gccccgacga tcatcgagga gaccgggctg accgcgtcca actcgatcgt gtactccatc
    19861 gcgatcggca tcatcaacct ggtgatgacg ctgatcgcga tccgcgtcat cgaccggtcg
    19921 gggcgccgcc cgctgctgct gttctcgctc ggcgcgatga cggtgatgat ggcgctgctc
    19981 gggctgtcgt tcgtcgcgga ctggtcgtcc gggctgtcgc tggtgtgcat ggtgctctac
    20041 atcgccggct acgcggtcgg caccgggccg gtgttctgga cgctgatcgg ggagatcttc
    20101 ccgccgcgcg cccgcgccgc cgggtccagc gcgtccaccg cggtgaactg gctggcgaac
    20161 ttcgtggtca gcctgatctt cctgccgatc gcgaacgtga tcgggcaggg cgagacgttc
    20221 tggatcttcg cggtgatcgc ggcggccggg tgggtcttcg tgagccgctt cgtcccggag
    20281 accaaggagc gcgacttcac ccaggtcgac gcggacctgc ggtcccggtt cgggcggccc
    20341 gcgcccgcgt ccgccgaccc ggactagccg cgctccgccg gaccggtcag gcggggaacg
    20401 ggccgggcat ccgctcccac cggcgccgga cgtcgtccca cgggaagcgc ggaccggggt
    20461 cgatgcggcg gcgcaccggc acgtccgggt cgtcggacgc ggggaccgtc tcctggtcca
    20521 ggtcgctgtg ccgcaccagg tgggcgagcg cggggtgggt ctccttgagg acggtcagca
    20581 gcccgatgag cgcggccgtc tggtccggcg ggaaatcctc gtccggctcc tgcgaccgcg
    20641 aatggaaatg gtccggatgg cggccggtat tgaccagctc gatgccgatc gacggggtgt
    20701 tgtgcccggc cgcgtgggcg gcgacccggt cgagcggcac gaaccggtgg cagccgccgt
    20761 cgcggtcgac gtagagatgg ccgcagaccc cggtgccgtc ctcggtcccg tcggcgctgc
    20821 gctcggcgta gtcccacgcc tcgtccagcg tcgggatctc cgtcgcgtgc aggacgacgg
    20881 tcgtgatcga ctccggggcc cgccgcttca gcgaccccca ccaggagacg agccggtcgt
    20941 ccacgggcgg gcctgcgggc gcggtcatga tccccatgct agcggcgaca aactgcagaa
    21001 aaggcttcta tgccattggg ttgccattat ttggcgaaat ggctgcgagg atggcatctc
    21061 acgcatatcg gagcgccagc ggggactctc cctggcgccg gagcatcggg ggatgccgtg
    21121 agcgatctga tcgccgtcgc ctatccggac gtcgacaccg cggtgacggt gcgcgaccgg
    21181 ctgatcgaca tgcaacgccg gaaactcatc acgctcgggg acgccgcggt ggtggagaag
    21241 cgccccgacg gaaagatcaa gctgcaccag atccacagca ccacgcggtc gggcgccctc
    21301 tggggcggcg tctggggcgg cctgctcggc atgctgttct tcgcgccgct ggtcggcgcc
    21361 gccgtcggcg cggcggccgg ggccgcgggc ggctccatga ccgacgtcgg cgtggacgac
    21421 tcgttcatgc gggacgtcgg cgagaagctc cgccccggcg gcgcggtcct gttcctgctg
    21481 gtcatctccg tcacgccgga ccgcgtcatc ccgcagatcg ccgagtacgg cggcgaggtg
    21541 atgcagacgt cgctgagcaa cgaggccgag gcgaacctcc gcgaggccct caaggcggcg
    21601 cagcacgcac aacccgccgg ggcgtcgccc cactgagcac cgcgcccctg gggccggccc
    21661 gccgccgggt cgccccaggg gcgtcactcc ccgccgtcgt cgtccggctc atcgggttcg
    21721 gccagctcgg gctcggccag ccgcgcgctc agccgatgcg acacgcgaac gtcgcggtac
    21781 gtcacgttca cttcggcggg ccggggcagg ttctcccgct tcgtgcgggc gcccgactcc
    21841 ccctccgggt cggcgcccgc ccgctcctcg acgtccggcg tctcgcggaa cacgacctcg
    21901 tccgcctcga cggaggcgcc gatctccaca tccggttccc gcatcgttcc ctcctcggtt
    21961 cgcctagatc cccggcagcc gcttgagcac gccgccgatc aactcccggc cggcgcggac
    22021 ggacttcctg gtcagcgccc ccaccagctc ccccgccgcc agatcatccg gcctctccgg
    22081 accgtgctgc cgctggtccc ggggctcgcg ttcatcccgc cgcccgcgcg cctccggccg
    22141 cccgtcctgg tccccatggt cacgccggcc tcgccgatcc tgctcgcgcc gccgttcatg
    22201 ctggtcccgg ggctcacgcc ggtcttctcg ctgttcgcgc acgtccggcc gcccgcgcag
    22261 gctcggccgc tcgtcctcgt cccagtggtc gcgccggttc cgatggccgc gctcgtgccg
    22321 ccgttcgcgc tcgccctcgg gcccatgccg atctcgccgc tcgtgctggt cccgcggctc
    22381 gcgttcgagt cgccgcccgt gcaagtccgg ccattcgtcc tggtcccagg ggtcgcgcca
    22441 gtcgtactcg gctcgacggt cgtgcgcgtg cccgcggtcg cggtgctcag gtccctggga
    22501 cgctgggccg gagtcgcggc ccacgtgggg gcggcgcggg cgctcggtgt ccgggccgtt
    22561 gcgcggccgt gccggctcgg cgcgcggcag ggcgcgtccg gcgtccgggc cctcgacggc
    22621 gcgggccgct tcggcggcgc cgctccccgc acgggcgagg agttcggggt tggcgtccac
    22681 cgtcgccagc acgcggtcga tgatctgcgc gacgttgtcg agccgcacct tgagcatggc
    22741 ctgcgcctcg acgccgcgga gggtgaggtt gacacggccg agcgag
//
