LOCUS       NZ_CP053564            10909 bp    DNA     circular CON 29-MAR-2024
DEFINITION  Pseudonocardia broussonetiae strain Gen 01 chromosome, complete
            genome.
ACCESSION   NZ_CP053564
VERSION     NZ_CP053564.1
KEYWORDS    RefSeq.
SOURCE      Pseudonocardia broussonetiae
  ORGANISM  Pseudonocardia broussonetiae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7177725)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2020) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013155125.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 06:16:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,087
            CDSs (total)                      :: 7,032
            Genes (coding)                    :: 6,962
            CDSs (with protein)               :: 6,962
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 19 of 70
            Pseudo Genes (incomplete)         :: 48 of 70
            Pseudo Genes (internal stop)      :: 6 of 70
            Pseudo Genes (multiple problems)  :: 3 of 70
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:38:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2607951
            Orig. end                         :: 2618860
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053564.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10909
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10909
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10909
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5909](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5909)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(11..1240)
                     /locus_tag="HOP40_RS12955"
                     /old_locus_tag="HOP40_12955"
     CDS             complement(11..1240)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1296: tellurium
                     resistance protein, TerD"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF021641.4"
                     /locus_tag="HOP40_RS12955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12955"
                     /product="VWA domain-containing protein"
                     /protein_id="WP_172168277.1"
                     /transl_table=11
                     /translation="MVATPRTPDVDASALLLDAGGRVRSDADLVFYNQPEHATGAVRHT
                     GKATAGDTLSVDLGRVEDAVDRIVLAASADGGTFGDVPGLLLRLTDADGGGVVAEFAMT
                     ASTETAFVGGELYRRGGAWKFRAVGQGYASGLAGLATDYGITVDDEPVATPAPEAGAGL
                     PIDVRKRLDLRKRQVAVSLEKRGARGVAARVVLALDASGSMSRLYRDGVVGRVVERMAA
                     VAAALDDDGTMQAWTFATNPARLPDLVVDDLPEWIRLHARVGEFRIRKRPPRDLEPGQV
                     DMAAVGGSNDEPKVIAQIRAFVRAAPVAVPTLVLFFSDGGVTRNREIERELRDAVPEPV
                     FWQFVGLGRRNRYGVLEKLDTMTGRRVDNVGFFAVDDIDDLPDADLYDRLLAEFPSWIT
                     DARAAGVLRR"
     gene            complement(1382..1858)
                     /locus_tag="HOP40_RS12960"
                     /old_locus_tag="HOP40_12960"
     CDS             complement(1382..1858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241159.1"
                     /locus_tag="HOP40_RS12960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12960"
                     /product="hypothetical protein"
                     /protein_id="WP_172158117.1"
                     /transl_table=11
                     /translation="MPPDPLVTTHVHTDGPIPGPHSLLTLTSAAHTGGGVLISTFSANV
                     RELPGATLHPGALSHWRSRADDWLHSRRASRPPALAMTDYARWIDQFPGAVYVADPART
                     DHLFVYWYLQRYTGRWPFAGTLLDAAVHSRLECSALCSLTGCRAPHAAPLAATS"
     gene            complement(2082..2627)
                     /locus_tag="HOP40_RS12965"
                     /old_locus_tag="HOP40_12965"
     CDS             complement(2082..2627)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /locus_tag="HOP40_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12965"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_172158120.1"
                     /transl_table=11
                     /translation="MDPVEINAGAWYLRVPRADDRMDDRHAVLASCSDPEITRWRHRPP
                     PDPAEADAYVRRRLRDWARDERYTWAVCEPTTGEMLGEVSLDRVDLPMAQAEAACWALP
                     AARRRGMTTTALGAVLRFGFAGLGLHRVTYMWAAGNTASGRVAEKCGFRTEGRMRDAWV
                     VDGERHDVIVTGLLATDR"
     gene            complement(2679..4451)
                     /locus_tag="HOP40_RS12970"
                     /old_locus_tag="HOP40_12970"
     CDS             complement(2679..4451)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677309.1"
                     /locus_tag="HOP40_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12970"
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="WP_172158122.1"
                     /sec_met_domain="ATP-grasp (E-value: 2.6e-08, bitscore:
                     32.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 2.2e-06,
                     bitscore: 26.0, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRLTKVLVANRGEIAVRVIRACADAGIGSVAVYADPDRDAPFVR
                     LADEAFALGGSTAAESYLDITKVVDAAKQAGADGIHPGYGFLSENADFAQAVMDAGITW
                     IGPTPQAIRDLGDKVTARHIATRAGAPLVPGTNDPVSGADEVIAFAKEHGLPVAIKAAF
                     GGGGRGMKVAREEKEIAELYDSAVREATAAFGRGECFVERYLDKPRHVEAQVLADTHGN
                     VIVVGTRDCSLQRRFQKLVEEAPAPFLSDEQRATIHEASKAICKEAEYHGAGTVEFLVG
                     QDGLISFLEVNTRLQVEHPVSEETSGLDLVREQFRIAEGLELNLLEDPEPRGHSIEFRI
                     NGEDAGRNFLPAPGTVSSISFPAGPGVRVDAGVESGSVIGGQFDSLLAKLIVTGSDRAQ
                     ALERSRRALAEFRVEGMATVLEFHRLVVEDPAFTGASEDEFTVHTRWIETEWENTVPPF
                     EGGEGADEETAPRQSVVVEVGGRRLEVSLPGDIAFGGGGGGAAAAKAPRKRGGGKGGSA
                     ASGDSVTAPMQGTIIKVAVSDGDTVSAGDLVVVLEAMKMENPVTAHKDGTVTGLNAEQG
                     ASVTQGTVLCEIKD"
     misc_feature    4497..4514
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.95"
                     /tool="antismash"
     gene            complement(4585..5004)
                     /locus_tag="HOP40_RS12975"
                     /old_locus_tag="HOP40_12975"
     CDS             complement(4585..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677308.1"
                     /locus_tag="HOP40_RS12975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12975"
                     /product="hypothetical protein"
                     /protein_id="WP_172158125.1"
                     /transl_table=11
                     /translation="MSVVWGPDAPSARDRLAARQAALVEALVAGGPPPPGFDATRLDAT
                     RCALLRKRAGAAAELWPLLAASFGARWSAVFAEHRDGHEPVGALRDGWDVARAVTGLTG
                     GAAAELAAREAAFRYDGRHPPRPRLRTRLRRMRRR"
     gene            complement(5001..5909)
                     /locus_tag="HOP40_RS12980"
                     /old_locus_tag="HOP40_12980"
     CDS             complement(5001..5909)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677307.1"
                     /locus_tag="HOP40_RS12980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12980"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_240157648.1"
                     /sec_met_domain="DUF692 (E-value: 3.1e-79, bitscore: 264.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAAADARAAGPGPARLAGVGIGWRAAIAGVVADVDGLGFTEVVA
                     ESLAPADPPRGVAALRERGVCVVPHGVRLSLGGTDPLDPARVTHLAACAAGVGAPLVSE
                     HVAFVRAGGREAGHLLPLPRSRDALDVLAANVARTQAELDVPLALEPIAALLDWPDDAM
                     TEGEFLTELLDRTGALLLLDVANVHANAVNRGQDPEAVLDAFPLDRIAYVHVAGGATHD
                     DGLYHDTHTAPVPDAVLRLLGALVDRLPAPPPVMLERDGRYPPAAELHAELAAIRGAAR
                     LEPPATREPPASRALVARESR"
     gene            complement(5893..6777)
                     /locus_tag="HOP40_RS12985"
                     /old_locus_tag="HOP40_12985"
     CDS             complement(5893..6777)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HOP40_RS12985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12985"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_172158130.1"
                     /transl_table=11
                     /translation="MDTWGISGPTFLTGYVLLAAVVWVAAARRRRALAEGSPRTAHDLG
                     GHEVAHLNGGPELAVTSALTAMHLRGTIAPAKGLVHAVGRLEPGAHPLERAVHFTCGSP
                     VPRARLPLHRPVRTALDDIHARLVAQGLLLSEPRRREIRATGWWVGAVALLGLLRLLAG
                     IADSRPVGYLVAVLLLVCVVTAVLLARAPRRSRAGDELLARLRSEHDALRPDSRPDWVA
                     YGPAAAALGVGIFGASAVWASDPAFAEELAVQKATAPGGDGGASFSGGGDGGGSGDGGG
                     GGGGGGCGGGCGG"
     misc_feature    complement(6801..6816)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.28"
                     /tool="antismash"
     gene            complement(6883..7314)
                     /locus_tag="HOP40_RS12990"
                     /old_locus_tag="HOP40_12990"
     CDS             complement(6883..7314)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224170.1"
                     /locus_tag="HOP40_RS12990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12990"
                     /product="SufE family protein"
                     /protein_id="WP_205347174.1"
                     /transl_table=11
                     /translation="MSLPPQLEELVEDFSGVGPKDRLQLLLELSRELPELPERYADAAD
                     SMEQVHECQSPLFLAVEVDDDADRRVHLFFSAPPEAPTTRGFASIMHTGMDGEPASAVL
                     AVPDDFYTALGLAQAVSPLRLRGMAAMLSRIKKQVRAAV"
     gene            complement(7311..8201)
                     /locus_tag="HOP40_RS12995"
                     /old_locus_tag="HOP40_12995"
     CDS             complement(7311..8201)
                     /EC_number="2.8.1.-"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224172.1"
                     /locus_tag="HOP40_RS12995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_12995"
                     /product="sulfurtransferase"
                     /protein_id="WP_172158133.1"
                     /transl_table=11
                     /translation="MALQNDTDPKLAEYAHPDRLVTTTWLADNLGADGLVVVESDEDVL
                     LYDTGHIPGAVKVDWHTELNDPVTRDYVDGARFAEICGERGITRDTTVVFYGDRNNWWA
                     TYALWVFSLFGHPDVRILDGGRAKWAAEGREMTTEQPKPEAVAYPVVERDDAAIRAFKD
                     DVLAHLGKPLVDVRSPGEYSGELLHMPDYPQEGAVRGGHIPGAASVPWARAAAEDATFR
                     SRAELEAIYLEEQGLKADDDVVAYCRIGERSSHTWFVLTHLLGFDKVRNYDGSWTEWGN
                     AVRVPIVQGSERGSA"
     gene            complement(8254..9393)
                     /locus_tag="HOP40_RS13000"
                     /old_locus_tag="HOP40_13000"
     CDS             complement(8254..9393)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) azdH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /locus_tag="HOP40_RS13000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_13000"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_172158136.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.9e-09, bitscore:
                     36.9, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="azdH (E-value: 6.5e-21, bitscore: 73.1,
                     seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.2e-12, bitscore:
                     44.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.8e-06, bitscore:
                     26.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLEQSRTRITDDEARSLAAGIGRAAAPFDADHDRDATFVAEAY
                     AAMAERGYLRLAVPTDLGGLGAGSRQVVLATEELGTHSGSAALASAMHQYLTLVQCWRR
                     RHGAPDAEAVLRRVATEDLVMATSGGSDWVCPTTTATAVEGGYRLDGRKVFCTQAPVAG
                     VVSTSAVLGPPGPDAVVLHAGVPLSAPGVSIVETWDTLGMRGTASHDLVFDGVFVPAEK
                     VLGRRPYGVLAGPLLVAAIHFAPVAAAAYLGVARGAVEEATRLVAARGVPSASTVRLVG
                     EAHSRLRVARWALLAAVDEVGEDPAADEDVLLTLMTTKRHAVTEARAVVDLTLEIAGGS
                     AFFRTSPLERAYRDVRGGAFHPLTPEATLELAGRRALAV"
     gene            9467..10162
                     /locus_tag="HOP40_RS13005"
                     /old_locus_tag="HOP40_13005"
     CDS             9467..10162
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672879.1"
                     /locus_tag="HOP40_RS13005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_13005"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_172158139.1"
                     /transl_table=11
                     /translation="MSRYGQYCPITRSLELLGDRWTLLVVRDLLVGATRFNELARGLPG
                     CSRGLLSKRLGQLERGGLVVHDEEGYRPTQACEELRPLIFGLAEWGARWAFGEPRADEL
                     DPTVLMWWIRGGIDPAPFGDRRTVLHVRVPDARRSRFWFVVQPHDVSLCFTDPGHEVDV
                     TVESTLSVLYQVWEGVLELPAAVRDGLVVLHGRRELVVRLPEALRFSPVAPFVRRARVT
                     APEALPTGR"
     gene            complement(10128..10754)
                     /locus_tag="HOP40_RS13010"
                     /old_locus_tag="HOP40_13010"
     CDS             complement(10128..10754)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796087.1"
                     /locus_tag="HOP40_RS13010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_13010"
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="WP_172158142.1"
                     /transl_table=11
                     /translation="MRVVLASASPARLSVLRAAGLDPLVQVSDVDEDALLASIPDATPA
                     EKVTTLAGAKATTVARRIEETEAVVLGCDSMLHIGGDLVGKPGTVDEARTRWRAMAGGT
                     GELVTGHAVLRVADGEIDRVAEGHAVTVVRFGEPTEEELDAYLGTGEPLAVAGAFTLDG
                     LGGWFVEGVDGDPSNVIGISLPLVRRLLAGVGVGVTDLWAAPPAP"
ORIGIN
        1 tgatcacggg tcagcgacgc agcacaccgg ccgcccgggc gtcggtgatc caggacggga
       61 actcggcgag cagccggtcg tagaggtcgg cgtcgggcag gtcgtcgatg tcgtcgacgg
      121 cgaagaagcc gacgttgtcg acgcgcctgc cggtcatcgt gtccagcttc tccagcacgc
      181 cgtaccggtt gcggcggccg agcccgacga actgccagaa caccggctcc gggacggcgt
      241 cgcggagttc gcgctcgatc tcgcggttgc gggtgacgcc gccgtcggag aagaacagga
      301 cgagcgtcgg gaccgcgacg ggcgccgccc gcacgaaggc ccggatctgc gcgatcacct
      361 tcggctcgtc gttggacccg ccgaccgcgg ccatgtcgac ctggcccggc tcgaggtccc
      421 gcggcggccg cttgcggatc cggaactccc cgacccgcgc gtgcagccgg atccactcgg
      481 gcaggtcgtc cacgacgagg tcgggcaggc gggccgggtt cgtggcgaac gtccaggcct
      541 gcatcgtgcc gtcgtcgtcg agggcggcgg cgacggcggc catgcgctcc acgacccggc
      601 ccacgacgcc gtcgcggtag agccggctca tggagcccga cgcgtcgagc gccagcacca
      661 cccgggccgc gaccccgcgg gcaccccgct tctccagcga caccgcgacc tggcgcttgc
      721 gcaggtcaag ccgcttccgc acgtcgatcg gcaggccggc gccggcctcc ggggcgggcg
      781 tcgccaccgg ctcgtcgtcg accgtgatcc cgtagtcggt ggccagcccg gccagcccgg
      841 aggcgtagcc ctgacccacc gcccggaact tccacgctcc gccccgccgg tagagctcac
      901 cgccgacgaa cgcggtctcc gtcgacgccg tcatggcgaa ctcggcgacg acgccgcccc
      961 cgtcggcgtc ggtgagccgc agcagcaggc ccgggacgtc gccgaacgtg ccgccgtcgg
     1021 ccgacgcggc gagcacgatg cggtcgacgg cgtcctcgac ccgtccgagg tcgacgctca
     1081 gcgtgtcgcc ggccgtcgcc ttcccggtgt ggcgcacggc ccccgtcgcg tgctcgggct
     1141 ggttgtagaa cacgaggtcg gcgtcggacc ggacgcggcc gccggcgtcg agcagcaggg
     1201 ccgacgcgtc gacgtcggga gtccggggcg tggcgaccag gtcagggcga cgtcgaggcg
     1261 ccgggcgtcg acggcggtgt tggcgccctt cgtcagtgcg gtcatgccgg ggatcctgcc
     1321 gggagccccg gcgggaacga cgaacggccg gcgcgtccgc ggacgcgccg gccgtcgccg
     1381 gtcagctggt ggccgccagc ggcgccgcgt gcggcgcccg gcagccggtc agtgagcaga
     1441 gggccgagca ctcgaggcgg ctgtgcacgg ccgcgtcgag cagggtgccc gcgaacggcc
     1501 agcggccggt gtagcgctgc aggtaccagt agacgaagag gtggtcggtg cgggcggggt
     1561 cggcgacgta gacggcgccg gggaactggt cgatccaccg ggcgtagtcg gtcatcgcga
     1621 gggcgggggg acgggaggcc cggcgggagt gcagccagtc gtcggccctg gaccgccagt
     1681 gggacagggc tcccgggtgg agcgtggctc cgggcagttc gcgcacgttc gcggagaacg
     1741 tgctgatcag gaccccgccg ccggtgtgcg cggccgaggt cagggtgagc agggagtgcg
     1801 ggcccgggat gggcccgtcg gtgtggacgt gcgtggtcac gagcgggtcg gggggcatga
     1861 cggatcctgg tcgcctggaa ggtcggggac gtcccacggc gtggcctgag ccgacgtggt
     1921 gggggatccg cggcgcggtc gtccgcgtgg cggaggaacc gggccccgtg ggtggacaca
     1981 cgggttcatc actgctgtcc gctatgcact tgtgcagcac ggtgcggggt cgctgtggct
     2041 gacctggccc gacggacgct agacgggcgc gcgcgacggc gtcaacggtc cgtcgccagg
     2101 aggccggtga cgatcacgtc gtgccgctcg ccgtcgacga cccaggcgtc gcgcatccgg
     2161 ccctcggtgc ggaacccgca cttctccgcg acccgcccgg aggcggtgtt gcccgccgcc
     2221 cacatgtagg tgacgcggtg cagcccgagc ccggcgaagc cgaagcgcag gacggcgccg
     2281 agcgcggtgg tcgtcatgcc gcggcgccgg gccgcgggca gcgcccagca ggcggcctcg
     2341 gcctgggcca tcggcaggtc gacgcggtcg agcgagacct cgcccagcat ctcgccggtg
     2401 gtcggctcgc agaccgccca cgtgtagcgc tcgtcgcggg cccagtcgcg caggcggcgc
     2461 cggacgtagg cgtcggcctc ggccgggtcg ggcggcgggc ggtggcgcca gcgcgtgatc
     2521 tcggggtcgg agcacgaggc gaggacggcg tggcggtcgt ccatgcggtc gtcggcgcgg
     2581 ggcacccgca ggtaccaggc gccggcgttg atctcgacgg ggtccatgac gaggtcgtga
     2641 gcggcaaccg tggccagggc cacggttgcc gcgcacggtc agtccttgat ctcgcagagc
     2701 acggtgccct gggtgaccga cgcgccctgc tcggcgttca gccccgtcac cgtgccgtcc
     2761 ttgtgcgccg tgaccgggtt ctccatcttc atcgcctcca ggacgacgac gaggtcgccc
     2821 gcggagacgg tgtcgccgtc ggacaccgcg accttgatga tcgtgccctg catcggcgcg
     2881 gtgaccgagt cgcccgacgc cgcgctgccg cccttgccgc cgccgcgctt gcgcggggcc
     2941 ttggccgccg cggcgccgcc gcccccgccg ccgaacgcga tgtcgccggg cagggagacc
     3001 tcgaggcggc gcccgccgac ctcgaccacg accgactgcc gcggcgcggt ctcctcgtcg
     3061 gcaccctcgc cgccctcgaa cggcgggacc gtgttctccc actcggtctc gatccaccgc
     3121 gtgtggacgg tgaactcgtc ctcggaggcg ccggtgaagg ccgggtcctc gacgacgagc
     3181 cggtggaact ccagcaccgt ggccatgccc tcgacgcgga actcggccag cgcgcggcgc
     3241 gagcgctcca gcgcctgcgc gcggtcggag ccggtgacga tgagcttggc cagcagcgag
     3301 tcgaactgcc cgccgatgac ggaccccgac tccacgcccg cgtcgacgcg cacgcccggc
     3361 ccggccggga acgagatcga cgagacggtg ccgggcgcgg ggaggaagtt gcggcccgcg
     3421 tcctcaccgt tgatccggaa ctcgatcgag tgcccgcgcg gctccgggtc ctccagcagg
     3481 ttgagctcga ggccctcggc gatgcggaac tgctcgcgga cgaggtcgag cccggaggtc
     3541 tcctccgaga ccgggtgctc cacctgcagg cgcgtgttga cctccaggaa cgagatcagc
     3601 ccgtcctggc cgacgaggaa ctcgacggtg ccggcgccgt ggtactcggc ctccttgcag
     3661 atggccttgg acgcctcgtg gatcgtggcg cgctgctcgt cggacaggaa cggcgcgggc
     3721 gcctcctcga cgagcttctg gaagcggcgc tgcagcgagc agtcgcgggt gccgacgacg
     3781 atgacgttgc cgtgcgtgtc ggccagcacc tgcgcctcga cgtggcgggg cttgtcgagg
     3841 tagcgctcga cgaagcactc gccgcgcccg aacgccgcgg tggcctcgcg gaccgccgag
     3901 tcgtacagct cggcgatctc cttctcctcg cgcgcgacct tcatgccgcg gccgccgccg
     3961 ccgaacgcgg ccttgatcgc gacggggagc ccgtgctcct tcgcgaacgc gatcacctcg
     4021 tcggcgcccg acacggggtc gttggtgccg gggacgagcg gcgcgcccgc gcgggtggcg
     4081 atgtggcggg cggtgacctt gtcgccgagg tcgcggatgg cctgcggcgt cggcccgatc
     4141 caggtgatgc cggcgtccat gaccgcctgg gcgaagtcgg cgttctcgga gaggaagccg
     4201 tagccggggt ggatgccgtc ggcgccggcc tgcttcgcgg cgtcgaccac cttggtgatg
     4261 tcgaggtagg actccgcggc ggtcgacccg ccgagcgcga acgcctcgtc ggccagccgg
     4321 acgaacgggg cgtcccggtc ggggtcggcg tagaccgcga cgctgccgat cccggcatcg
     4381 gcgcaggccc ggatcacgcg gacggcgatc tccccgcggt tcgcgacgag gaccttggtc
     4441 aagcgcggca cgttgcggcc tccctgagat gaccggggaa cccctgcccc ggcgcgtggt
     4501 taccggggag tttaggcatg accggcccga tcgccggacg gaggaccgtg tggcgtcgat
     4561 gacgttgcgc ccgaccgcgg gaggtcagcg cctgcgcatc cggcgcagcc gcgtgcgcag
     4621 ccgggggcgc ggcgggtggc ggccgtcgta gcggaacgcg gcctcgcggg cggccagctc
     4681 ggccgcggcg ccgcccgtga gcccggtgac cgcgcgcgcc acgtcccagc cgtcgcgcag
     4741 ggccccgacc ggctcgtggc cgtcgcggtg ctcggcgaac accgcgctcc agcgcgcgcc
     4801 gaaggacgcc gcgagcagcg gccagagctc ggcggccgcg ccggcccgct tgcgcagcag
     4861 ggcgcagcgg gtggcgtcga ggcgggtggc gtcgaagccg ggcgggggcg ggccgccggc
     4921 caccagggcc tcgacgaggg cggcctggcg ggcggcgagg cggtcgcggg ccgagggcgc
     4981 gtccgggccc cagacgacgc tcaccgcgac tcacgcgcga cgagagcgcg actcgcgggg
     5041 ggttcccgag tcgcgggggg ttccaggcgg gccgcgcccc ggatcgccgc cagctcggcg
     5101 tgcagctccg cggcgggcgg gtagcggccg tcgcgctcca gcatcaccgg cggcggggcg
     5161 gggaggcggt cgaccagcgc cccgagcagc cgcagcacgg cgtcggggac gggcgcggtg
     5221 tgggtgtcgt ggtagagccc gtcgtcgtgc gtggcgccgc ccgcgacgtg gacgtaggcg
     5281 atgcggtcga gcgggaacgc gtcgagcacc gcctccgggt cctgcccgcg gttcaccgcg
     5341 ttggcgtgca cgttggcgac gtcgagcagc agcagggcgc cggtgcggtc gagcagctcg
     5401 gtgaggaact cgccctcggt catcgcgtcg tcgggccagt cgagcagcgc ggcgatcggc
     5461 tccagcgcca gcgggacgtc cagctcggcc tgcgtgcgcg ccacgttggc ggcgaggacg
     5521 tcgagcgcgt cgcgggagcg ggggagcggc agcaggtgcc cggcctcgcg gccccccgcc
     5581 cggacgaacg cgacgtgctc gctcaccagc ggcgccccga cgccggccgc gcaggcggcg
     5641 aggtgcgtga cgcgggcggg gtcgagcggg tcggtgccgc ccagcgacag ccgcaccccg
     5701 tgcggcacga cgcacacccc ccgctcccgc agagccgcca cccctcgagg cggatctgcg
     5761 ggagcgaggg actcggcgac gacctcggtg aagccgaggc cgtcgacgtc ggccaccacg
     5821 ccggcgatcg cggcccgcca gccgatcccc acgccggcga gccgggcggg tccgggaccg
     5881 gcggcccggg cgtcagccgc cgcagccacc gccgcaccct ccgccgcccc cgccgccccc
     5941 gccatcgccg ctgcccccgc cgtcgccgcc cccggagaag gaggcgccgc cgtcaccgcc
     6001 cggggccgtg gccttctgca cggcgagctc ctcggcgaag gccgggtcgg acgcccacac
     6061 ggcgctcgcc ccgaagatcc cgacgccgag cgcggccgcg gcggggccgt aggccaccca
     6121 gtcgggccgg gagtccggcc gcagggcgtc gtgctcggac cgcaggcgcg ccaggagctc
     6181 gtcacccgcg cgggagcgcc gcggggcccg ggccagcagc acggccgtga cgacgcagac
     6241 cagcagcagc accgcgacga ggtacccgac gggcctgctg tcggcgatgc cggcgagcag
     6301 gcgcagcagc cccagcagcg cgacggcgcc gacccaccag cccgtcgcac ggatctcgcg
     6361 gcggcgcggc tccgagagca gcaggccctg ggcgacgagg cgggcgtgga tgtcgtccag
     6421 cgcggtccgc acggggcggt gcaggggcag ccgggcgcgg ggcaccgggc tgccgcaggt
     6481 gaagtggacg gcccgctcga gcgggtgggc tccgggctcg aggcggccga ccgcgtgcac
     6541 cagccccttg gccggggcga tcgtgccgcg caggtgcatg gccgtcagcg cggaggtgac
     6601 ggccagctcg gggccgccgt tgaggtgggc cacctcgtgc ccgcccaggt cgtgggcggt
     6661 acgaggtgac ccctcggcga gcgcacggcg ccgccgcgcg gccgcgaccc acaccaccgc
     6721 ggccaggagc acgtagccgg tgaggaaggt ggggccgctg attccccagg tgtccatcgc
     6781 gcacctcctg aagacgtcct catgagacgc ctcacagtgt tgcgaccggc accgtccggg
     6841 ggaacgttct tcaccaggac ttgaggtggc ccgggtgcgg gcctacacgg cggcgcggac
     6901 ctgcttcttg atccgcgaga gcatcgcggc catgccgcgc agccgcagcg ggctcacggc
     6961 ctgggcgagc ccgagcgcgg tgtagaagtc gtcgggcacg gcgagcacgg ccgacgccgg
     7021 ctccccgtcc atccccgtgt gcatgatcga ggcgaacccg cgcgtggtcg gggcctcggg
     7081 cggcgcggag aagaacaggt gcacgcgccg gtcggcgtcg tcgtcgacct cgacggcgag
     7141 gaacagcggc gactggcact cgtgcacctg ctccatcgag tcggccgcgt cggcgtagcg
     7201 ctcgggcagc tccgggagct cgcgggagag ctccaggagc agctgcaggc ggtccttcgg
     7261 gccgacgccg gagaagtcct cgacgagctc ctccagctgc ggaggcaggc tcacgccgag
     7321 ccccgctcgc tgccctgcac gatcggcacg cggaccgcgt tgccccactc ggtccagctg
     7381 ccgtcgtagt tgcggacctt gtcgaagccg agcaggtgcg tgagcacgaa ccaggtgtgg
     7441 ctggagcgct cgccgatgcg gcagtaggcg acgacgtcgt cgtcggcctt gaggccctgc
     7501 tcctccaggt agatcgcctc gagctccgcc cgggagcgga acgtggcgtc ctcggcggcc
     7561 gcgcgggccc acggcacgct ggccgcgccg gggatgtggc cgccgcggac ggcgccctcc
     7621 tgcgggtagt cgggcatgtg cagcagctcg ccggagtact cgcccgggct gcggacgtcg
     7681 accagcggct tgcccaggtg cgcgagcacg tcgtccttga acgcgcggat cgcggcgtcg
     7741 tcgcgctcca cgaccgggta cgcgacggcc tcgggcttgg gctgctcggt ggtcatctcg
     7801 cggccctcgg cggcccactt ggcgcgcccg ccgtcgagga tccgcacgtc ggggtggccg
     7861 aacaggctga acacccacag cgcgtaggtg gcccaccagt tgttgcggtc gccgtagaac
     7921 acgacggtgg tgtcgcgggt gatgccgcgc tcgccgcaga tctcggcgaa gcgggcgccg
     7981 tcgacgtagt cgcgggtgac cgggtcgttg agctcggtgt gccagtcgac cttcacggcc
     8041 cccgggatgt ggccggtgtc gtagaggagg acgtcctcgt cggactcgac gacgacgagg
     8101 ccgtcggcgc ccaggttgtc ggccagccac gtcgtcgtga ccaggcggtc ggggtgcgcg
     8161 tactcggcca gcttcgggtc ggtgtcgttc tgcagagcca tgggaccagg ctagtccggc
     8221 ggcgacggtt ccgtcgccgc cggtgcgggg ccctcacacc gccagcgcgc gccgtccggc
     8281 gagctccagg gtggcctcgg gcgtgagcgg gtggaaggcg ccaccgcgca cgtcgcggta
     8341 ggcgcgctcc agcggcgagg tgcggaagaa ggcgctgccg cccgcgatct ccagggtgag
     8401 gtcgacgacg gcccgcgcct cggtcacggc gtgccgcttg gtcgtcatca gggtgagcag
     8461 gacgtcctcg tcggcggccg ggtcctcgcc cacctcgtcg acggcggcca gcagcgccca
     8521 gcgcgccacc cgcagccggg agtgggcctc cccgacgagc cggacggtcg acgccgacgg
     8581 gaccccgcgg gcggcgacca gccgcgtggc ctcctccacc gcgccgcgcg cgaccccgag
     8641 gtaggcggcc gcggccaccg gcgcgaagtg gatcgcggcc accagcagcg gcccggcgag
     8701 cacgccgtag ggccgcctgc ccagcacctt ctccgcgggc acgaacacgc cgtcgaacac
     8761 gaggtcgtgg ctcgccgtgc cgcgcatgcc gagcgtgtcc caggtctcga cgatcgagac
     8821 gcccggagcc gacagcggca ccccggcgtg caggacgacc gcgtccgggc cgggcgggcc
     8881 cagcaccgcc gacgtggaga cgacgccggc caccggggcc tgggtgcaga acaccttgcg
     8941 cccgtcgagc cggtagccgc cctcgacggc ggtggccgtc gtcgtcgggc acacccagtc
     9001 cgagccgccg ctggtggcca tcacgaggtc ctcggtcgcg acgcggcgca gcaccgcctc
     9061 cgcgtcgggc gccccgtggc ggcgccgcca gcactggacg agcgtcaggt actggtgcat
     9121 ggcggacgcg agcgcggccg agccggagtg cgtgcccagc tcctcggtgg cgaggacgac
     9181 ctggcgcgac ccggcaccga gcccgccgag gtcggtcggc accgccaggc gcaggtagcc
     9241 gcgctcggcc atggcggcgt aggcctcggc gacgaaggtg gcgtcgcggt cgtggtcggc
     9301 gtcgaacggg gcggcggcac ggccgatgcc ggcggccagc gaccgcgcct cgtcgtcggt
     9361 gatgcgggtg cgggactgct ccagagtggt catgggaccg agcgtccggt gtaaccgggg
     9421 ccgcggatag ttccggatct gcaccggccg gaggacgatc cacgccgtgt cccgctacgg
     9481 ccagtactgc ccgatcaccc ggtcgctgga gctgctcggc gaccgctgga ccctgctcgt
     9541 cgtccgggac ctgctggtcg gtgccacccg cttcaacgag ctggcgcgcg ggctgcccgg
     9601 ctgctcgcgc gggctgctgt ccaagcggct gggccagctc gagcgcggcg ggctggtcgt
     9661 gcacgacgag gagggctacc ggcccacgca ggcgtgcgag gagctgcggc cgctgatctt
     9721 cgggctggcc gagtggggcg cgcggtgggc gttcggcgag ccgcgggccg acgagctcga
     9781 cccgacggtg ctgatgtggt ggatccgcgg cggcatcgac ccggccccgt tcggcgaccg
     9841 gcgcaccgtg ctgcacgtcc gggtgcccga cgcccgccgg tcgcggttct ggttcgtggt
     9901 gcagccgcac gacgtgtcgc tgtgcttcac cgaccccggg cacgaggtcg acgtgaccgt
     9961 ggagtcgacg ctctcggtgc tctaccaggt gtgggagggg gtgctggagc tcccggccgc
    10021 cgtccgcgac gggctcgtgg tgctgcacgg gcgccgcgag ctggtggtgc gcctgcccga
    10081 ggcgctgcgg ttctccccgg tggcgccgtt cgtccggcgc gcgcgggtca cggcgccgga
    10141 ggcgctgccc acaggtcggt gaccccgacg ccgacgcccg ccaggagccg ccgcaccagg
    10201 ggcaggctga tcccgatgac gttggacggg tcgccgtcga cgccctccac gaaccagccg
    10261 ccgagcccgt cgagggtgaa cgcgcccgcc accgcgagcg gctcgcccgt ccccaggtag
    10321 gcgtcgagct cctcctcggt cggctcgccg aaccggacga cggtgacggc gtggccctcg
    10381 gcgacgcggt cgatctcccc gtcggcgacg cggagcacgg cgtgcccggt gacgagctcg
    10441 cccgtgccgc cggccatcgc gcgccagcgg gtgcgggcct cgtcgacggt gccgggcttg
    10501 ccgaccaggt cgccgccgat gtgcagcatc gagtcgcagc ccaggaccac ggcctcggtc
    10561 tcctcgatcc ggcgcgccac cgtggtggcc ttggcgccgg cgagcgtcgt gaccttctcg
    10621 gccggggtgg cgtccgggat cgacgcgagg agcgcgtcct cgtcgacgtc ggacacctgc
    10681 acgagcgggt ccagccccgc cgcgcgcagg accgagaggc gggcggggga ggccgaggcc
    10741 agtacgacgc gcacgacgcc ggaccctacg gccgggtccg gcccgggcgt cggggggccg
    10801 tgacctggcc cgcgccgcac acgtcgtacc gccctgcggt gctcgccggg acggggccgt
    10861 ggagcgctgg gccgtacgga gcagtcgaga ttgctccgcg gcgggaggc
//
