LOCUS       NZ_CP053564            10408 bp    DNA     circular CON 29-MAR-2024
DEFINITION  Pseudonocardia broussonetiae strain Gen 01 chromosome, complete
            genome.
ACCESSION   NZ_CP053564
VERSION     NZ_CP053564.1
KEYWORDS    RefSeq.
SOURCE      Pseudonocardia broussonetiae
  ORGANISM  Pseudonocardia broussonetiae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7177725)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2020) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013155125.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 06:16:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,087
            CDSs (total)                      :: 7,032
            Genes (coding)                    :: 6,962
            CDSs (with protein)               :: 6,962
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 19 of 70
            Pseudo Genes (incomplete)         :: 48 of 70
            Pseudo Genes (internal stop)      :: 6 of 70
            Pseudo Genes (multiple problems)  :: 3 of 70
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:38:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2101038
            Orig. end                         :: 2111446
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053564.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10408
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10408
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10408
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5408](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5408
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    35..49
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            957..1772
                     /locus_tag="HOP40_RS10370"
                     /old_locus_tag="HOP40_10370"
     CDS             957..1772
                     /NRPS_PKS="Domain: PKS_KR (7-170). E-value: 1e-14. Score:
                     46.8. Matches aSDomain:
                     nrpspksdomains_HOP40_RS10370_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666739.1"
                     /locus_tag="HOP40_RS10370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10370"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_172157098.1"
                     /sec_met_domain="adh_short (E-value: 1.4e-34, bitscore:
                     118.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEFAGRVAVVTGAGSGIGRALARDLAGRGARLALSDVDKDGLAE
                     TVASITGAEVRSDALDVTDRAAVLAYADEVVGQFGRVNQVYNNAGIAFAGNVLTSEFEE
                     IERVVDIDFWGVVNGTKAFLPHLIASGDGHVVNVSSLFGLLSVPSQSAYNAAKFAVRGF
                     TESLRQEMLVARHPVQVTCVHPGGIRTGIARNAARGKGFEDGAAMERFDRQMLRMSPEK
                     AASIILAGVRRNRARVLVGNDAKVLDAVVRLLGSAYQRPFAAVAGRTLR"
     aSDomain        978..1466
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HOP40_RS10370_PKS_KR.1"
                     /evalue="1.00E-14"
                     /label="HOP40_RS10370_PKS_KR.1"
                     /locus_tag="HOP40_RS10370"
                     /protein_end="170"
                     /protein_start="7"
                     /score="46.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAGSGIGRALARDLAGRGARLALSDVDKDGLAETVASITG
                     AEVRSDALDVTDRAAVLAYADEVVGQFGRVNQVYNNAGIAFAGNVLTSEFEEIERVVDI
                     DFWGVVNGTKAFLPHLIASGDGHVVNVSSLFGLLSVPSQSAYNAAKFAVRGFTESLRQE
                     "
     CDS_motif       984..1052
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HOP40_RS10370_0001"
                     /evalue="5.50E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="HOP40_RS10370"
                     /protein_end="32"
                     /protein_start="9"
                     /score="19.0"
                     /tool="antismash"
                     /translation="VVTGAGSGIGRALARDLAGRGAR"
     gene            1790..2965
                     /locus_tag="HOP40_RS10375"
                     /old_locus_tag="HOP40_10375"
     CDS             1790..2965
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330635.1"
                     /locus_tag="HOP40_RS10375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10375"
                     /product="MFS transporter"
                     /protein_id="WP_240157613.1"
                     /transl_table=11
                     /translation="MDQVRTRTAPGLLVALAFLATMLGTTLPTPLYPIYQQELGFGGLM
                     VTVVFATYAVGVLAALLLFGRLSDRIGRRPVLLPGLALAAASSLVFLVPDSLPALFVGR
                     LLSGLSAGVFTGVATAAIVDLAPPEGRARAGLLAAAVNMLGLGLGPVLAGALADLAPLP
                     LVLPYLVHLGLVAVGAVAVLFVPEPVVRQPGPVRLEVQRLSVPADVRPVFVRAGIAGFA
                     GFSVLGFFTAVSPLFVSGVLREPSHLLSGLVVFSLLGSSTAGQLLSARLPLRTSLLGGC
                     LALAAGIAVVGAGLATSSLAVLVAGAVVAGLGQGASFRAGLQSVTGTAPAERRSEVSSS
                     FFLLLYVAISIPVIGVGAGAQVFGLVPTAVVFAGLVALLALGAFTSLLRRV"
     misc_feature    complement(3087..3101)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     misc_feature    complement(3102..3117)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            3194..3730
                     /gene="ectA"
                     /locus_tag="HOP40_RS10380"
                     /old_locus_tag="HOP40_10380"
     CDS             3194..3730
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700303.1"
                     /locus_tag="HOP40_RS10380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10380"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_172157100.1"
                     /transl_table=11
                     /translation="MSALGTTAPETGSDAPTTTSPPTTADGVALWEIAAASRVLDVNSR
                     YAYVLWCRDFAATSIVARIGERVVGFVTGYVRPERPDTLFVWQVAVHEVARGRRVAATM
                     LDDLVARVGTPFLETTVTDDNAASLAMFAGLAERTGADMERTELFGPAELGAEHDPEFL
                     YRIGPMQLTPTPTRG"
     gene            3739..5004
                     /gene="ectB"
                     /locus_tag="HOP40_RS10385"
                     /old_locus_tag="HOP40_10385"
     CDS             3739..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 1.1e-68.
                     Score: 223.7. Matches aSDomain:
                     nrpspksdomains_HOP40_RS10385_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="HOP40_RS10385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10385"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_172157102.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-70, bitscore:
                     236.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVFEDLESEVRSYCRNWPVVFDTARGSRLTDVDGRTYLDFFAGA
                     GALNYGHNPPALKKPLLEYLSRDGITHGLDMYTTAKGEFLRTFEDRILRPRGLDYKVQF
                     PGPTGANTVESALKLARKISGREAMINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDNYFDGTVPDFLWMEKLLQDAGSGLNEPAAVIVETVQGEGGINPARVEWLQGLD
                     ALCKRNGILLIVDDVQMGVGRTGPFFSFEIAGIQPDIVCISKSISGYGLPMALTLIKPE
                     HDVWGPGEHNGTFRGNNPAFVTATAALNEFWSDDALERSTLAKGERVDQAFTEIASRSQ
                     TVELTPKGRGLARGLQFSQPELAAKACAAAFERGLLMETSGPSDEVMKILPALTITDDE
                     LEEGLAIVGESVAAVTEGVSAR"
     aSDomain        3805..4809
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HOP40_RS10385_Aminotran_3.1"
                     /evalue="1.10E-68"
                     /label="HOP40_RS10385_Aminotran_3.1"
                     /locus_tag="HOP40_RS10385"
                     /protein_end="357"
                     /protein_start="22"
                     /score="223.7"
                     /tool="antismash"
                     /translation="DTARGSRLTDVDGRTYLDFFAGAGALNYGHNPPALKKPLLEYLSR
                     DGITHGLDMYTTAKGEFLRTFEDRILRPRGLDYKVQFPGPTGANTVESALKLARKISGR
                     EAMINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDNYFDGTVPDFLWMEKL
                     LQDAGSGLNEPAAVIVETVQGEGGINPARVEWLQGLDALCKRNGILLIVDDVQMGVGRT
                     GPFFSFEIAGIQPDIVCISKSISGYGLPMALTLIKPEHDVWGPGEHNGTFRGNNPAFVT
                     ATAALNEFWSDDALERSTLAKGERVDQAFTEIASRSQTVELTPKGRGLARGLQF"
     gene            5001..5408
                     /locus_tag="HOP40_RS10390"
                     /old_locus_tag="HOP40_10390"
     CDS             5001..5408
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="HOP40_RS10390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10390"
                     /product="ectoine synthase"
                     /protein_id="WP_172157104.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.8e-51, bitscore:
                     170.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTERDVKTENWRSKRIVLGGDGVGFSVHETVLQAG
                     TVNDFWYANHIEAVFITEGEGELYDKDNDTTYALAPGSIYVLNGNERHQLRPRTQMRTV
                     CVFNPPVTGREVHDENGVYPLITAEEEKEAS"
     gene            5411..6319
                     /gene="thpD"
                     /locus_tag="HOP40_RS10395"
                     /old_locus_tag="HOP40_10395"
     CDS             5411..6319
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806918.1"
                     /locus_tag="HOP40_RS10395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10395"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_172157106.1"
                     /sec_met_domain="PhyH (E-value: 7.2e-38, bitscore: 129.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVTTRRRTARIGDRYPTRVADRPVIIDRAEPAVWSTDPGMLTPD
                     ELAAHERDGFVTVPQLLTPDEVGEYAAELDRLATDPAVRADERTIVEKTSQQVRSVFEV
                     HVLSERIAALVADERVAGRARQILGSEVYVHQSRINYKPGFGGAGFYWHSDFETWHAED
                     GMPSPRAVSISIALTENYPHNGCLMIMPGSHKEFVACVGETPADHYKESLREQEIGTPD
                     PESLTALADRHGIAMLTGAAGSAMLFDSNCMHGSGSNITPYPRSNIFVVFNSVENGLEE
                     PFSAPGPRPSHVAARQIVPVG"
     gene            complement(6336..7106)
                     /locus_tag="HOP40_RS10400"
                     /old_locus_tag="HOP40_10400"
     CDS             complement(6336..7106)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682490.1"
                     /locus_tag="HOP40_RS10400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10400"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_172157108.1"
                     /transl_table=11
                     /translation="MRTWNSFVAIGDSFTEGLDDWRPDGSPRGWADRVAEEIAAGRPGF
                     RYANLAVRGKLLDQIVEDQIPVAERLRPELVSFCAGGNDLIRFGCDVDDLARRFDDALG
                     RIAATGAEVFIFTGFDLGRMHPLIRRLRGRVATFNESMRAAAERHGSHVVDLWGMAPLA
                     DPRAWGPDRLHLRPDAHRRVALRVLETLGEPVTEDWRAPVPDGDPMPWRARQADDLRWM
                     REFVMPFVRKRVQGRRTGDGFLPKRPDLLPFDRV"
     misc_feature    7063..7075
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            7233..7883
                     /locus_tag="HOP40_RS10405"
                     /old_locus_tag="HOP40_10405"
     CDS             7233..7883
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459637.1"
                     /locus_tag="HOP40_RS10405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10405"
                     /product="DUF3159 domain-containing protein"
                     /protein_id="WP_172157110.1"
                     /transl_table=11
                     /translation="MTTPEPSLADVLGGRGGAVDATVPVAAFVAGWGLAGALGWAGPVA
                     WGGGAAVLAAAVIGAVRLRAGTPPRAVLFGLLGVALAAVVALSTGRAADFFLVRIVSNA
                     VSALAWAVSIVVRWPLLGLVVGTALGQRTRWRRDPDLLRGYQRASWVWVAQYVVRLAVF
                     LPLYHADAVVALGIAQTVLTWPLVAVCIALSWPLVRSALPEGHGGIRHPAGPE"
     gene            7980..8696
                     /locus_tag="HOP40_RS10410"
                     /old_locus_tag="HOP40_10410"
     CDS             7980..8696
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HOP40_RS10410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HOP40_10410"
                     /product="hypothetical protein"
                     /protein_id="WP_172157112.1"
                     /transl_table=11
                     /translation="MPRPATPSDTSTVEITAEKKAPDLTVNKVLAGAGAAATAAVLGSY
                     LGAAGTVLGAALGSVASTIASVLYQRSLDRTRDTVVARIRPGRIPAQRGAPDAETVIIA
                     PVPPPPVGRRPWRRAVVAAIVVFVLGLAAVTGLEWANGSSLTRDESGTSVGRVIAPPPS
                     TDTDTTGDTTTTDEPTEDPTDEPTPTGDATEEEGDDEGTGDGPPVVEDSGEAEPTTAAP
                     TPTPTPTGSVRPSGGR"
     gene            complement(8710..9354)
                     /gene="thiE"
                     /locus_tag="HOP40_RS10415"
                     /old_locus_tag="HOP40_10415"
     CDS             complement(8710..9354)
                     /EC_number="2.5.1.3"
                     /GO_function="GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1280: thiamine-phosphate pyrophosphorylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097897.1"
                     /locus_tag="HOP40_RS10415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HOP40_10415"
                     /product="thiamine phosphate synthase"
                     /protein_id="WP_172157114.1"
                     /transl_table=11
                     /translation="MPGLDGDALRARLDDARLYLCTPARPDLAEFVDAALAGGVDVVQL
                     RDKGIEARDELAALEVLAEVCARHGALLAVNDRADVALAAGADVLHLGQDDLPVDWARR
                     VVGDEVVVGRSSHSADETLAAADEPGVDYFCVGPCWPTPTKPGRPAPGLGLVRTVADRA
                     PARPWFAIGGIDQERLTEVLDAGARRIVVVRAITEAEDPRAAAAALAARLR"
     regulatory      9378..9483
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    complement(9440..9453)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     misc_feature    complement(9651..9666)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
ORIGIN
        1 cccatgcgca ggcgctcggc gcgcagcagg tcgaggtcga cgtcggccac cgagcggcgc
       61 ggccccttcg ggaagcgctc ggtctcggcg agcagcacgc cgttctcgta gaccatcgtc
      121 tggccgtccc aggccaggtc ggtggtcgac tcgccctcgc ccgccgccgc gtaggcgtag
      181 gccgcgaggc agcgcgacga cgccgactgc gccagcagct tgcggtcgtc ggcccgcccc
      241 acggtgatcg ggctgccgga gaggttgacg acgacggtcg cccccgcgag cgtcgcctcc
      301 gcgctcggcg gcaccgggac ccacatgtcc tcgcacacct cggcgtgcag cacgagcccg
      361 ggcacgtcgt cggcggcgaa cagcaggtcg gggccgatcg gcacgtcggc gtcgcccagg
      421 cggacggtgc cgcgcacgtc gtcgccgggg gcgtactggc ggcgctcgta gaactcgcgg
      481 taggtcggca ggtacgactt gggcgcgatg ccgaggaccg cgccccggtg gatcaccacg
      541 gcgcagttga ggatgcggtg cagcacccgc agcggcgcgc cgacgacgag caccggccgc
      601 agccccgccg acgccgcggc gatgtcgagc agcgcgcggc gcacgtcgtc gagcaggacg
      661 tcctgcatga cgacgtcctc gagcgagtag cccgagagcg tgagctcggg gaacacggcg
      721 acggcgacgc cctggtcgtg gcactcccgc gccgtctcca gcacggcccg cgcgttggcg
      781 gccgggtcgg cgagggtggt gcgcagggtg caggcggcca cgcgcgcgaa gccgtggcgg
      841 tacgcggagc ggaagtccga cacgggccca gtctgccgca cgggtcgacg gctgtccggg
      901 tcggcggcgc gaccggggct ggcttgttac ccgtcggtag ggcaccatct gcgtccatga
      961 gcgagttcgc gggacgcgtc gccgtggtga cgggagccgg atcgggtatc ggacgggcgc
     1021 tggcccggga cctcgcgggc cgcggtgcgc ggctcgcgct gtccgacgtc gacaaggacg
     1081 ggctggcgga gacggtcgcg tcgatcaccg gcgcggaggt ccgcagcgac gccctcgacg
     1141 tgaccgaccg cgccgccgtg ctggcctacg ccgacgaggt ggtggggcag ttcgggcgcg
     1201 tcaaccaggt ctacaacaac gccggcatcg ccttcgccgg caacgtcctc acctcggagt
     1261 tcgaggagat cgagcgcgtc gtcgacatcg acttctgggg cgtggtcaac ggcaccaagg
     1321 cgttcctgcc gcacctgatc gcgtccggcg acgggcacgt cgtcaacgtc tccagcctgt
     1381 tcggcctgct gtcggtgccg agccagagcg cctacaacgc ggcgaagttc gcggtgcgcg
     1441 ggttcaccga gtcgctgcgc caggagatgc tcgtcgcgcg ccacccggtg caggtcacct
     1501 gcgtgcaccc cggcggcatc aggaccggga tcgcccgcaa cgccgcccgc ggcaagggct
     1561 tcgaggacgg cgccgcgatg gagcgcttcg accgccagat gctgcggatg agcccggaga
     1621 aggcggcgtc gatcatcctc gcgggggtgc ggcgcaaccg ggcccgcgtg ctcgtgggca
     1681 acgacgccaa ggtcctcgac gccgtggtgc gcctgctcgg ctcggcctac cagcgcccgt
     1741 tcgcggcggt cgcggggcgc acgctgcggt agcggcatac gctccgccag tggatcaggt
     1801 gcgcacgagg accgcgccgg gcctgctcgt cgccctcgcc ttcctcgcca cgatgctcgg
     1861 caccaccctg ccgacgccgc tctacccgat ctaccagcag gaactgggct tcggcggcct
     1921 gatggtcacg gtcgtgttcg cgacctacgc cgtcggggtg ctggcggcgc tgctgctgtt
     1981 cgggcggctg tccgaccgga tcggccgccg ccccgtgctg ctgcccggcc tcgcgctcgc
     2041 cgcggccagc tcgctggtgt tcctcgtgcc cgacagcctg cccgcgctgt tcgtcggccg
     2101 gctgctctcc gggctgtcgg ccggggtgtt caccggcgtc gcgacggcgg cgatcgtcga
     2161 cctggcaccg cccgagggcc gggcgcgcgc cgggctgctg gccgcggccg tgaacatgct
     2221 cgggctcggg ctcggcccgg tgctcgcggg cgcgctcgcc gacctggcgc cgctcccgct
     2281 ggtgctgccc tacctcgtgc acctgggcct ggtggccgtc ggtgcggtgg cggtgctgtt
     2341 cgtgccggag ccggtcgtgc ggcagcccgg gccggtgcgg ctggaggtgc agcggctgtc
     2401 ggtgcccgcc gacgtccgcc cggtgttcgt gcgcgccggc atcgcggggt tcgccgggtt
     2461 ctccgtgctc ggcttcttca ccgccgtctc gccgctgttc gtctcggggg tgctgcgcga
     2521 gccgagccac ctgctgtccg gtctcgtcgt gttctccttg ctcggcagct ccaccgcggg
     2581 ccagctgctc tcggcgcggc tgccgctgcg cacgtcgctg ctgggaggct gcctggcgct
     2641 ggccgccggg atcgccgtcg tcggcgccgg gctggccacc tcctcgctcg ccgtcctcgt
     2701 cgcgggggcg gtggtggccg ggctcgggca gggcgcgagc ttccgcgccg ggctgcagtc
     2761 ggtcaccggg acggcgcccg ccgagcggcg cagcgaggtc tcgtcgagct tcttcctgct
     2821 gctctacgtc gccatctcga tccccgtcat cggggtcggc gcgggcgcgc aggtgttcgg
     2881 gctcgtgccc accgccgtcg tgttcgccgg gctggtcgcg ctgctggcgc tcggggcgtt
     2941 caccagcctg ctccggcggg tatgacggtt gtgatggacc tcgcggacca cgatcgaggc
     3001 ccggacgccg acccgcgtac gccccgtcgg cggagccggt acggcactgc ggagcagcgg
     3061 gaacgccgtg ctagccgttt ccggtaatcg ataaacgcga tagtgatcgc gctcacagga
     3121 aatgcgcgga atgggttcct gatcgattcg ggaacgtcgc atcagggtcc cttcacctgt
     3181 aactttcatg gccatgtccg cactcggcac gaccgcaccg gagaccggga gcgacgcacc
     3241 gacgacgact tcgccgccca ccaccgccga cggcgtcgcc ctgtgggaga tcgccgcggc
     3301 ctcgcgggtg ctcgacgtga actcccgcta cgcctacgtg ctgtggtgcc gcgacttcgc
     3361 cgccacctcg atcgtcgcgc gcatcgggga gcgggtcgtc gggttcgtca ccggctacgt
     3421 ccggcccgag cgccccgaca cgctgttcgt ctggcaggtc gcggtgcacg aggtcgcccg
     3481 gggcaggcgg gtcgcggcga cgatgctcga cgacctcgtc gcgcgggtcg gcacgccctt
     3541 cctggagacg acggtgaccg acgacaacgc cgcgtcgctg gcgatgttcg ccgggctcgc
     3601 cgagcgcacg ggcgcagaca tggaacgcac ggagctgttc ggcccggccg agctcggtgc
     3661 ggagcacgac cccgagttcc tgtaccggat cgggccgatg cagctgacgc cgacacccac
     3721 gcgaggatga tgacgacgat gaccgtgttc gaggatctgg agtccgaggt ccggagctac
     3781 tgccgcaact ggcccgtggt gttcgacacc gcgcgcgggt cgcgcctgac cgacgtggac
     3841 ggccggacct acctcgactt cttcgccggc gccggggccc tgaactacgg ccacaacccg
     3901 cccgcgctga agaagccgct gctggagtac ctgtcccgcg acggcatcac gcacggcctc
     3961 gacatgtaca ccacggccaa gggcgagttc ctgcgcacgt tcgaggaccg gatcctgcgg
     4021 ccgcgcgggc tggactacaa ggtgcagttc cccggcccga ccggcgcgaa caccgtggag
     4081 tcggcgctca agctggcgcg caagatcagc ggccgcgagg cgatgatcaa cttcacgaac
     4141 gcgttccacg gcatgacgct gggcgcgctg tcggtcaccg gcaactcgat gaagcgcggc
     4201 ggcgcgggca tcccgctggt gcacgccacc ccgatgccct acgacaacta cttcgacggc
     4261 accgtccccg acttcctgtg gatggagaag ctcctccagg acgccgggtc gggcctcaac
     4321 gagcccgccg cggtgatcgt cgagacggtg cagggcgagg gcggcatcaa ccccgcgcgc
     4381 gtcgagtggc tgcagggcct cgacgcgctg tgcaagcgca acggcatcct gctcatcgtc
     4441 gacgacgtgc agatgggcgt cgggcgcacc gggccgttct tctccttcga gatcgccggg
     4501 atccagcccg acatcgtctg catctccaag tcgatcagcg ggtacggcct gcccatggcc
     4561 ctcacgctca tcaagcccga gcacgacgtg tggggccccg gcgagcacaa cggcacgttc
     4621 cgcggcaaca accccgcatt cgtcaccgcc accgccgcgc tgaacgagtt ctggagcgac
     4681 gacgccctgg agcgctcgac gctggccaag ggcgagcgcg tcgaccaggc gttcaccgag
     4741 atcgcgtccc gctcgcagac cgtcgagctg acgccgaagg ggcgcggcct ggcccgcggc
     4801 ctgcagttct cccagcccga gctcgccgcg aaggcgtgcg ccgcggcgtt cgagcgcggc
     4861 ctgctgatgg agacctccgg cccgtccgac gaggtcatga agatcctgcc ggccctgacc
     4921 atcaccgacg acgagctcga ggagggcctc gccatcgtcg gcgagtccgt cgccgccgtc
     4981 accgaagggg tgtccgcacg atgatcgtcc gcacgctcga cgagatcacc gacaccgagc
     5041 gcgacgtcaa gaccgagaac tggcggagca agcgcatcgt cctgggcggc gacggcgtcg
     5101 ggttctcggt gcacgagacg gtgctgcagg ccggcaccgt caacgacttc tggtacgcga
     5161 accacatcga ggccgtcttc atcaccgagg gtgagggcga gctgtacgac aaggacaacg
     5221 acacgaccta cgcgctcgcg cccgggtcga tctacgtgct gaacggcaac gagcgccacc
     5281 agctgcgccc gcgcacgcag atgcgcacgg tgtgcgtgtt caacccgccg gtcacgggcc
     5341 gcgaggtcca cgacgagaac ggcgtgtacc cgctgatcac ggcggaggag gagaaggagg
     5401 cttcgtaggc atggcagtga cgacccgacg acggacggcg cgcatcggcg accgctaccc
     5461 cacccgcgtg gcggaccgcc cggtcatcat cgaccgcgcg gagcccgcgg tctggagcac
     5521 ggacccgggg atgctcaccc cggacgagct cgcggcccac gagcgcgacg gcttcgtcac
     5581 ggtgccgcag ctcctgaccc ccgacgaggt gggcgagtac gcggccgagc tggaccggct
     5641 cgccaccgac cccgcggtgc gggccgacga gcgcacgatc gtcgagaaga cctcgcagca
     5701 ggtgcggtcg gtcttcgagg tgcacgtgct gagcgagcgg atcgcggcgc tggtcgccga
     5761 cgagcgcgtg gccgggcggg cccgccagat cctcggctcc gaggtctacg tgcaccagag
     5821 ccggatcaac tacaagcccg ggttcggcgg tgccggcttc tactggcact ccgacttcga
     5881 gacctggcac gccgaggacg ggatgccgtc gccgcgggcg gtgagcatct cgatcgcgct
     5941 caccgagaac tacccgcaca acggctgcct catgatcatg ccgggctcgc acaaggagtt
     6001 cgtggcgtgt gtcggggaga ccccggccga ccactacaag gagtcgctgc gcgagcagga
     6061 gatcggcacg cccgacccgg agagcctcac cgcgctggcc gaccgccacg gcatcgcgat
     6121 gctcaccggc gccgccggct cggccatgct gttcgactcc aactgcatgc acggctcggg
     6181 cagcaacatc acgccctacc cgcgctcgaa catcttcgtc gtgttcaaca gcgtggagaa
     6241 cgggctcgag gagccgttct cggcgccggg gccgcggccg tcgcacgtgg cggcgcggca
     6301 gatcgtgccg gtgggctgag accccgccgg gccggctaga cccggtcgaa cgggagcagg
     6361 tccggccgct tgggcaggaa cccgtcgccg gtgcgccggc cctgcacccg cttgcgcacg
     6421 aacggcatga cgaactcgcg catccagcgc aggtcgtcgg cctggcgggc gcgccacggc
     6481 atcgggtcgc cgtcgggcac cggggcgcgc cagtcctcgg tgaccggctc gccgagcgtc
     6541 tccagcaccc gcagcgccac gcggcggtgg gcgtcggggc gcaggtgcag ccggtcgggg
     6601 ccccacgcgc gggggtcggc gagcggggcc atgccccaga ggtcgacgac gtggctgccg
     6661 tggcgctcgg ccgcggcccg catcgactcg ttgaaggtgg ccacgcggcc gcgcagccgg
     6721 cggatgagcg ggtgcatccg gcccaggtcg aagccggtga agatgaagac ctcggcgccg
     6781 gtggcggcga tgcggccgag cgcgtcgtcg aagcggcggg cgaggtcgtc gacgtcgcag
     6841 ccgaagcgga tgaggtcgtt gccgcccgcg cagaacgaca cgagctcggg gcgcagccgc
     6901 tcggccaccg ggatctggtc ctcgacgatc tggtcgagca gcttcccgcg cacggcgagg
     6961 ttggcgtagc ggaagccggg gcgcccggcg gcgatctcct cggcgacgcg gtcggcccag
     7021 ccgcgagggg agccgtcggg gcgccagtcg tcgaggccct cggtgaagct gtcgccgatc
     7081 gccacgaagc tgttccaggt gcgcacgacg accgcctccg ttcacgtccg ctccatcccc
     7141 gcgacgggtg gaggtcatcc caggatcacc catcggcgca ccgtcgcgcc agtcccggtg
     7201 atcacttcgt gtcggtaggg caccctcagt acgtgaccac acccgaaccg tcgctcgccg
     7261 acgtcctcgg cgggcgcggt ggcgccgtcg acgcgaccgt gcccgtcgcc gcgttcgtgg
     7321 ccggctgggg tctcgccggc gcactgggct gggccgggcc ggtcgcgtgg ggcgggggcg
     7381 cggcggtgct cgcggccgcg gtgatcgggg ccgtccggct gcgggcgggg acgccgccgc
     7441 gggcggtgct gttcgggctg ctcggcgtcg cgctggcggc ggtggtggcg ctgtccaccg
     7501 gccgcgcggc cgacttcttc ctggtccgga tcgtcagcaa cgcggtgagc gcgctggcgt
     7561 gggcggtgtc gatcgtcgtg cggtggcccc tgctcgggct cgtcgtcggc acggcgctgg
     7621 ggcagcgcac ccgctggcgc cgcgaccccg acctgctgcg cggctaccag cgcgcgagct
     7681 gggtgtgggt ggcgcagtac gtcgtgcggc tggcggtgtt cctgccgctc taccacgccg
     7741 acgccgtcgt ggcgctgggc atcgcccaga ccgtgctgac ctggccgctc gtggccgtct
     7801 gcatcgcgct gtcgtggcca ctggtgcgct cggcgctgcc ggagggccac ggcgggatcc
     7861 ggcaccccgc cgggccggag tgaacgtcgc cactcggccg tgatcgtcgg aaacgatcca
     7921 gcggcgtcgt tcgctgaaaa gggtgacacc ccttcccgat ctggagcgcc tgccgagcca
     7981 tgccccgtcc cgccaccccg tccgacacct ccaccgtcga gatcacggcc gagaagaagg
     8041 cccccgatct gacggtcaac aaggtcctcg ccggagcggg ggccgcggcc acggccgcgg
     8101 tgctcggctc ctacctcggg gcggcgggga ccgtgctggg cgcggcgctc ggatcggtcg
     8161 cgagcacgat cgcctcggtg ctctaccagc gctcgctcga ccgcacccgc gacacggtcg
     8221 tcgcccgcat ccggcccggg cggatccccg cgcagcgcgg cgccccggac gccgagacgg
     8281 tgatcatcgc gccggtgccg ccgccgccgg tggggcgccg cccgtggcgc cgcgccgtcg
     8341 tggccgcgat cgtggtgttc gtcctgggcc tggccgccgt caccggtctg gagtgggcca
     8401 acggctcgtc gctgacgagg gacgagtcgg gcacctcggt cggccgcgtg atcgccccgc
     8461 cgccgtcgac ggacacggac accacgggcg acacgacgac gaccgacgag ccgaccgagg
     8521 accccaccga cgagccgacg ccgaccggcg acgcgaccga ggaggagggc gacgacgagg
     8581 ggaccggcga cggcccgccg gtcgtcgagg actccggtga ggccgagccg acgaccgccg
     8641 ccccgacgcc cacgccgacg ccgacgggct ccgtgcggcc gtcgggcggc cggtagccac
     8701 ccgacctggt cagcgcaggc gggcggccag cgccgccgcc gcggcgcgcg ggtcctccgc
     8761 ctcggtgatc gcgcgcacca cgacgatccg ccgcgcgccc gcgtcgagca cctcggtgag
     8821 ccgctcctgg tcgatgccgc cgatggcgaa ccaggggcgc gccggcgccc ggtcggccac
     8881 cgtgcgcacc aggccgaggc cgggggcggg ccggccgggc ttggtcggcg tcggccagca
     8941 ggggccgacg cagaagtagt cgacgcccgg ctcgtcggcg gccgccagcg tctcgtcggc
     9001 gctgtgcgac gagcgcccga ccaccacctc gtcgccgacg acccggcgcg cccagtccac
     9061 cggcaggtcg tcctgcccga ggtgcaggac gtcggcgccc gcggccagcg cgacgtcggc
     9121 gcggtcgttc accgcgagca gcgcgccgtg ccgcgcgcac acctccgcga gcacctccag
     9181 cgcggccagc tcgtcgcggg cctcgatgcc cttgtcgcgc agctgcacga cgtcgacgcc
     9241 gccggccagt gcggcgtcga cgaactccgc gaggtccggg cgggcgggcg tgcagaggta
     9301 gaggcgggcg tcgtcgagcc gcgcgcgcag ggcgtcacca tcgagtccgg gcacggacgt
     9361 gagcgtaggc tggcccccag gtgacgcacg ggagccccgg tggggctgag aggggaccac
     9421 ggagtcccga ccgtcgaacc tgatccgggt catgccggcg cagggagcgg ggagcatgca
     9481 gcaggtgatc gtgatcggcg cgggcgtgat cggcctgtcc tgcgcctggc gggcggccgc
     9541 cgcggggtgg ccggtgaccc tcgtggaccc ggcacccggg tccggggcgt cgtgggtggc
     9601 gggcgggatg ctcgcgcccg tcaccgaggc gtggccgggc gaggagcggc tgctcgacct
     9661 cggcgtcgcg tcgctggagc ggtggccggg cttcgccgcc gacctggggc cgtgcgggct
     9721 gcgcaccgag ggcaccgtcg tcgccgccac gggctcgggc gaccgcgccg agctcgacgc
     9781 cctcgccgcc cacctggcgg gcctgggccg cgacgtcgag cgcctctccg ggcgcgagct
     9841 gcgccggctc gaaccggcga tcgggcccga cgtgcggggc gggctgtgcg tgccggagga
     9901 cctcgccgtc gacaaccgcg ctctgctcgc cgcgctgcgc tcggccgcgg cccgggcggg
     9961 ggtgaggtcc gtggaggcgg ccgcggcggc cgtcgtcgac gacggggagc gcgtcaccgg
    10021 ggtgcgcctg gccgacggca cggtgctcgc cgcgcccgtc gtcgtcgtgg cggcgggggc
    10081 gcactccggc accctgcacc cggcgctgga cggcctcgtg cgcccggtga agggcgagat
    10141 cctgcggctc gcgcaccggc cgggcgcgtt cccgccgccg cggcggaccg tgcgcgcgct
    10201 ggtcgacggg cgtcccgtct acgccgtgcc gcgcgacggc ggcggcctcg tcatcggggc
    10261 gacgcagacc gagaccggct tcgacaccac cgtcaccgcg ggcggcgtcc gcgacctgct
    10321 gcgcgacgcc gagcgcgtgc tgcccggcgt cgccgagtac gcgctcgtgg agaccgcggc
    10381 cgggctgcgt cccggcaccc cggacaac
//
