LOCUS       NZ_JABBGE010000018     10804 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig18, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000018
VERSION     NZ_JABBGE010000018.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 72813)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 72813)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 33947
            Orig. end                         :: 44751
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10804
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10804
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10804
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5804](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5804)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            48..524
                     /locus_tag="HHL19_RS36045"
                     /old_locus_tag="HHL19_36055"
     CDS             48..524
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952304.1"
                     /locus_tag="HHL19_RS36045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36055"
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_169094641.1"
                     /transl_table=11
                     /translation="MTAGPEPISEAARAALSRELSELRAERAKVAATLDDADEPGDRAD
                     QADELQRATDADRLETRIAEIEGRLREASAAGAPSADTIGVGSTVGVRFGDGSESTLHI
                     GELADADDPALVTADSPLGRALLGHTAGDSIAYATPLGRTTAVVLSVGTPGGGS"
     gene            521..1354
                     /locus_tag="HHL19_RS36050"
                     /old_locus_tag="HHL19_36060"
     CDS             521..1354
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520635.1"
                     /locus_tag="HHL19_RS36050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36060"
                     /product="hypothetical protein"
                     /protein_id="WP_169094675.1"
                     /transl_table=11
                     /translation="MTGAADSRVDPDLAEERRADRKGGPGHTVLLALALAVPAAKVAYT
                     VGGGDAVRDVFVGMEPANWPDVLLGMVITDPLLGSVLAVVVSRVVFAVFAARGGLPFGG
                     GRLAHLRRVGLAILNPLAFGVIVTCLFGPWWGLGTTLAAYALRKGVVVEYRTGRRRPHH
                     GHGTAHGPSRAPDYRPPAWLSRAAAAEQGVALILTAVVLPLLFLASALDGQAWTSIVAC
                     RVTDGTRTTDARLIELSRKGTGVVGWNLESEEISNGVGCTSTESRYVREPWWRRP"
     gene            complement(1382..1747)
                     /locus_tag="HHL19_RS36055"
                     /old_locus_tag="HHL19_36065"
     CDS             complement(1382..1747)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982417.1"
                     /locus_tag="HHL19_RS36055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36065"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_169094642.1"
                     /transl_table=11
                     /translation="MQECAKAMRPEDITRLFVERSNAGDAAGVAALYAEDAVLAYPPGE
                     LTVGREAIRALWEKVLAHRPRFEREEPLPTLVNGDIALTSTPPKDGAGARAQVVRRQPD
                     GSWLRILDQPEFVPPAR"
     gene            1853..2743
                     /locus_tag="HHL19_RS36060"
                     /old_locus_tag="HHL19_36070"
     CDS             1853..2743
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104815.1"
                     /locus_tag="HHL19_RS36060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36070"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_169094643.1"
                     /transl_table=11
                     /translation="MDLRQLEYFVAVAEERNFTRAAERVHISQSGVSAQIRRLEHELGA
                     ELFDRSARAVALTAAGEAALAHARAALASAEAVGRAVDEVTGLVRGRLTVGMVVGCTLT
                     PLFDALAAFHAAHPGVEVLLQEDNSDRLAEGVRSGAVDLALIGAAAATPDGLDALTIID
                     ERIVAAVPHDHPLAGLPHLALRELTAHPLVCMPPGTGLRAVLDRACAEQDLRPSIALEA
                     GAADAIADLAARGLGVAVLSASMASVYADRLTALPLADVDIPALLALVWKPAHNPAVRT
                     LLTHARHAFGLTDTP"
     gene            complement(2848..4320)
                     /locus_tag="HHL19_RS36065"
                     /old_locus_tag="HHL19_36075"
     CDS             complement(2848..4320)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675308.1"
                     /locus_tag="HHL19_RS36065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36075"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_169094644.1"
                     /sec_met_domain="DAO (E-value: 1.9e-09, bitscore: 29.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSTRPRPARPAGSTPTAVVVGAGPNGLAAGALLAEAGLEVTVLE
                     AAPTIGGGTRTDEAILPGLLHDHCSAVHPLAVASPALRHLRLDRYGLNWRLPEVDCAHP
                     LDDGTAAALYRSVTETAAGLGADAARYRALLAPSVRRWEALSADVMGPLLRVPDHPLLL
                     ARFGLPTVLPAAVLARLFRTPGARALWAGVAAHAYRPLTDPLSSAIGLGILTAGHAAGW
                     AVAEGGSRSITDALAAALRAHGGRIETGVRITHRSQLPPADLTLLDLDPAQVAAVYGDL
                     LPSRTAAAYRRFRRGPGAFKVDLAVEGGVPWRAEAARRAGTVHLGGPLAEIVRAERDVT
                     DGRMPERPFVLVGQQYLADPSRSVGDVHPVWTYAHVPHGYDGDATDAILARLEQFAPGV
                     RDRVVGLRVTRPADFAAANPNFAGGDILTGAKTVPQLVLGARPALDPYATGLPGVYLCS
                     AATPPGPGAHGMSGAGAAATALRHLGIRPPWR"
     gene            complement(4363..5004)
                     /locus_tag="HHL19_RS36070"
                     /old_locus_tag="HHL19_36080"
     CDS             complement(4363..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763512.1"
                     /locus_tag="HHL19_RS36070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36080"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_169094645.1"
                     /transl_table=11
                     /translation="MTVGRAWSWRYAVPRLAGAGAVGAAAGAVTGALADTALGILAGIA
                     AAETVFVVTGWIVLWPMDAAATRRNLRREEFRPVVEELAVVTAALCGLVAIVALLLVDS
                     GYDRTAAATALWGVFMAWAALHLMYATRYAYLYYAPPEGGVDFNSDDHPRYTDFLYFSY
                     NLGMTYQVSDTNVSDSGIRAIVLRHCLLSYVFGASILATAINLVAGIVTG"
     gene            complement(5001..5804)
                     /locus_tag="HHL19_RS36075"
                     /old_locus_tag="HHL19_36085"
     CDS             complement(5001..5804)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135064.1"
                     /locus_tag="HHL19_RS36075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36085"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_169094646.1"
                     /sec_met_domain="Linocin_M18 (E-value: 8.5e-92, bitscore:
                     298.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSNLHRELAPITPAAWDEIEEEARRTFRRTVAGRRVVDVPDPGG
                     AGLAAVGTGHLTDLAAPAAGVRARLREARPLVELRVPFTVDRSAVDDVERGAKDSDWQP
                     VKDAARTLALAEDSVVFDGYEAAGVRGLRAASSNLVVALPTEPRDYPDAVSRALTALRL
                     AGVDGPYALLLGADEYRAVSETSDHGYPIAAHLGRMLDGPPIWAPALTGGYVLSTRGGD
                     FELRLGQDVSIGYTRHDADTVELYLHETLTYFTYTDEAVVVLGGA"
     gene            complement(5801..6952)
                     /locus_tag="HHL19_RS36080"
                     /old_locus_tag="HHL19_36090"
     CDS             complement(5801..6952)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834462.1"
                     /locus_tag="HHL19_RS36080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36090"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_169094647.1"
                     /transl_table=11
                     /translation="MAAPQTTPQPAPDPTPHPTPGASPHPTPQPVPQLILGPPAKAALF
                     LTLTVAPGRETDVREGLGGLSGLARSVAFRARDDALACVAGLASDAWDRLVGGPRPREL
                     HPFTPLQGPRHRAPATPGDLLLHLRARRADLCFELARVVLDGFGDAVTVVDEVHGFTYF
                     DDRDLLGFVDGSENPTGPSAAGAVLVADEDPGFRGGSYVVVQKYLHDLTAWNALSTEEQ
                     ERVIGRSKADNVELPDDVKPSDSHVALNTVEDEDGEERQIVRQNMPFGRLGAGEFGTYF
                     IGYARTPAVTEQMLRNMFLGDPPGNTDRILDFSTAVTGGLFFVPSADLLDDLPAPEAAR
                     ETAPEPAAEAQAVPAVAPAAVPVDPAAAAVGLGIGSLKGVPAS"
     gene            7131..9446
                     /locus_tag="HHL19_RS36085"
                     /old_locus_tag="HHL19_36095"
     CDS             7131..9446
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515925.1"
                     /locus_tag="HHL19_RS36085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_36095"
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="WP_169094648.1"
                     /transl_table=11
                     /translation="MSLDRKQQDGLEPRVGPVPDGEPEVRPYHHPAAGWGAAKSVTRFM
                     AREGALPVDGPRAIMKMNHERTGFDCPGCAWPDDTKGLHLDICENGIKHVTWEMTRKRV
                     GRDFFAAHSVTELAGWTDYALENEGRLTEPMVYDPGTDHYVPIGWKDAFELVGSALRGL
                     DDPNQASFYTSGRLGNEATFLYQLLARELGTNNLPDCSNMCHEASGRALTASLGTGKGT
                     VDLADWETADALFILGVNAASNAPRMLTALAEAYHRGAQIVHVNPLVEAAATRTIIPHD
                     FRDMATFRATRTSTLNLQPRIGGDMALVRGMAKAVLEQSASDPKALDPEFIERHTAGFE
                     EYRAVCEATPWEEIELQSGLPREEILKAARVYREADRSIVSWCLGVTQHEHGVDTVREI
                     VNLLLLRGNLGREGAGPSPVRGHSNVQGNRTCGIDHRPTAAFLDRLGEVCGIDPPRAHG
                     LDTVRTIEAMGRGDVKVFVGMGGNFALAAPDTPVTYEALRNCELTVQVSTKLNRSHVVH
                     GRRALILPCLGRTEKDHQRKGEQATSVEDSMSMVHLSRGMKKPASAHLLSEPAIVAGMA
                     RAALPDSTTPWAWYVEDYDRIRDTMARVLDGFEDFNRRVRLPLGFRIKQPARELVFRTP
                     SGKAEFSAAPLPDVVPEPGTLALGTMRSHDQWNTTIYSDDDRYRGVSGLRTLVFMNRAD
                     MRERGITELAPVDITATARDGSRRSVHGYLAVPYDIPRGCAAGYMPEMNVLCALGDYST
                     QSDQPIMKHLKVVIEPTG"
ORIGIN
        1 tgggaggcgt cgtccttcgt ccccgccacc gcacgacggg agcagtcatg accgctggac
       61 cggagcccat cagcgaggcc gcccgcgcgg ctctgagccg cgaactgtcc gagctgcgcg
      121 ccgagcgggc gaaggtcgcc gccacgctcg acgacgccga cgagcccggc gaccgggccg
      181 atcaggccga cgagttgcag cgggccacgg acgcggaccg gctggagacg cggatcgcgg
      241 agatcgaggg gcggctgcgg gaggcctcgg ccgccggtgc gccgtcggcc gacacgatcg
      301 gcgtgggcag cacggtgggc gtgcggttcg gcgacggctc ggagtcgacg ctgcacatcg
      361 gcgagctggc cgacgcggac gatccggccc tggtgaccgc cgacagtccg ctgggccggg
      421 cgctgctcgg ccacacggcc ggcgacagca tcgcgtacgc cactcccctg ggccgtacca
      481 ccgccgtcgt cctctcggtc ggcacgccgg gcggcggctc ctgaccggcg cggccgacag
      541 ccgcgtcgac ccggacctcg cggaggagcg ccgggccgac cgcaagggcg gcccggggca
      601 cacggtgctc ctcgccctgg cgctcgccgt gcccgcggcc aaggtcgcgt acacggtggg
      661 cggcggggac gcggtgcggg acgtgttcgt cggcatggag ccggcgaact ggccggacgt
      721 gctgctcggc atggtgatca cggatccgct gctcggctcg gtcctcgcgg tcgtggtgtc
      781 ccgggtcgtc ttcgccgtct tcgcggcgcg cggcggcctg ccgttcggcg gcggccggct
      841 cgcccatctg cgccgggtcg ggctcgccat cctgaacccg ctggcgttcg gcgtgatcgt
      901 cacctgtctc ttcggcccgt ggtggggcct gggcacgacc ctcgccgcgt acgcgctccg
      961 caagggcgtg gtggtggagt accgcacggg acggcgccgc ccccaccacg gacacggcac
     1021 cgcgcacggg ccctcgcgcg caccggacta ccggccgccc gcgtggctga gccgggccgc
     1081 ggccgcggag cagggcgtgg ccctgatcct caccgccgtc gtcctgcccc tcctgttcct
     1141 ggcctccgcg ctcgacggcc aggcctggac ctcgatcgtc gcctgccggg tcaccgacgg
     1201 gacccgcacc accgacgccc gtctgatcga gctgagccgc aagggcaccg gcgtggtcgg
     1261 ctggaacctg gagtccgagg agatctccaa cggcgtcggc tgtaccagta cggagagccg
     1321 ttacgtacgg gagccctggt ggcggcgtcc gtgacggcca cgcgcgcgtg cgccgcccgg
     1381 ctcagcgggc cggcgggacg aactcgggct ggtcgaggat ccgcagccag ctgccgtcgg
     1441 gctgacgccg gacgacctgg gcccgcgcgc cggcgccgtc cttcggcggg gtcgaggtga
     1501 gggcgatgtc gccgttcacc agtgtgggca gcggctcctc ccgctcgaag cggggccggt
     1561 gggcgaggac cttctcccac agcgcccgga tggcttcccg ccccaccgtc agctcgccgg
     1621 gcgggtacgc gagcacggcg tcctcggcgt agagcgcggc gactccggcg gcgtcgccgg
     1681 cgttggaccg ctcgacgaag aggcgggtga tgtcctccgg gcgcatggcc ttcgcgcact
     1741 cctgcatcgg gttcctcctg agctgtttgc gtcgtccttg gtgcctccag cctcacaagc
     1801 cgtcagccag aagtccaaca gatggtggtg ctacgtacta gaatttccag tcatggattt
     1861 gaggcagctg gagtacttcg tggcggtcgc cgaggagcgg aacttcaccc gggccgccga
     1921 gcgggtgcac atcagtcagt cgggcgtcag cgcgcagatc cggcggctcg aacacgagct
     1981 gggcgccgag ctgttcgacc gctcggctcg ggccgtcgcc ctcaccgccg ccggggaggc
     2041 ggcgctcgcg cacgcccgtg cggcgctcgc ctcggcggag gcggtcggcc gggcggtcga
     2101 cgaggtcacg ggcctggtgc ggggccgact gacggtcggc atggtcgtcg gctgcaccct
     2161 gaccccgctg ttcgacgccc tggccgcctt ccacgcggcc catcccgggg tggaggtcct
     2221 gctccaggag gacaactccg accggctcgc cgaaggcgtc cggagcggcg ccgtcgacct
     2281 ggcgctcatc ggcgccgccg ccgcgacccc cgacggcctg gacgcgctga cgatcatcga
     2341 cgaacggatc gtcgcggccg tccctcacga ccaccccctc gcgggcctcc cccacctcgc
     2401 cctccgcgag ctgacggccc acccgctcgt ctgcatgccc ccgggcaccg gcctgcgcgc
     2461 ggtcctcgac cgggcgtgcg cggagcagga cctccgcccg tcgatcgccc tggaggcggg
     2521 cgccgcggac gcgatcgccg acctcgccgc ccggggcctc ggcgtcgccg tcctcagcgc
     2581 gtcgatggct tcggtctacg ccgaccgcct caccgcgctc cccctcgccg acgtcgacat
     2641 cccggccctc ctcgccctgg tctggaaacc cgcccacaac ccggccgtac gcaccctcct
     2701 cacccacgcc cgccacgcct tcggcctgac cgacacgccc tgacggaacc cgtcggatcc
     2761 gcggcccgcg cgccgcgccg gaaggggccg cgaggggccg tcacgggggc gtgcgggggt
     2821 cgcggccggg tgcgctgacg gtcggcgtca ccgccagggc ggcctgatgc ccaggtggcg
     2881 gagggccgtc gcggcggcgc cggcgccgga catgccgtgg gcgccggggc cgggcggggt
     2941 ggcggcggag cagaggtaga cgccggggag gccggtggcg taggggtcga gggcggggcg
     3001 ggcgcccagg accagttggg ggacggtctt ggcgccggtg aggatgtcgc cgccggcgaa
     3061 gttggggttg gcggcggcga agtcggcggg gcgggtgacg cgcaggccga cgacgcggtc
     3121 gcggacgccg ggggcgaact gttccaggcg ggccaggatc gcgtcggtcg cgtcgccgtc
     3181 gtagccgtgc ggcacgtgcg cgtacgtcca caccgggtgg acgtcgccga cggagcggga
     3241 ggggtcggcg aggtactgct ggccgacgag gacgaagggg cgctccggca tccggccgtc
     3301 cgtgacgtcc cgttcggcgc ggacgatctc ggcgagcggg ccgccgaggt ggaccgtgcc
     3361 ggcccggcgg gcggcctccg cgcgccacgg caccccgccc tccaccgcca ggtcgacctt
     3421 gaaggcgccg gggccgcgcc ggaagcggcg gtacgcggcg gccgtacggg acggcaggag
     3481 gtcgccgtac acggccgcga cctgggccgg gtcgaggtcg aggagcgtga ggtcggcggg
     3541 cggcagctgg gagcggtggg tgatcctgac gcccgtctcg atccggccgc cgtgggcgcg
     3601 gagggcggcc gcgagggcgt cggtgatgga gcgggagccg ccttcggcga cggcccagcc
     3661 cgccgcgtgc ccggcggtca ggatgccgag tccgatggcg gagctgagcg ggtcggtgag
     3721 cgggcggtag gcgtgggcgg cgacgcccgc ccacagcgcg cgggcgcccg gggtgcggaa
     3781 gaggcgggcg agcacggcgg cgggcaggac ggtcggcagg ccgaagcggg ccagcaggag
     3841 gggatggtcg gggacgcgca ggagcgggcc catgacgtcc gcggagagcg cctcccagcg
     3901 gcgtaccgag ggggccagca gggcccggta gcgggccgcg tcggcgccga gcccggcggc
     3961 ggtctcggtg accgagcggt acagggcggc ggcggtgccg tcgtcgaggg ggtgggcgca
     4021 gtcgacctcg gggagccgcc agttcagccc gtaccggtcg aggcgcaggt ggcggagggc
     4081 gggcgaggcg acggcgaggg gatgcacggc cgagcagtgg tcgtggagca gtccgggcag
     4141 gatcgcctcg tcggtgcggg tgcctccgcc gatcgtcggc gcggcctcca ggacggtgac
     4201 ctcgaggccg gcctcggcga gcagggcgcc ggccgcgagc ccgttgggac cggcgccgac
     4261 gacgacggcg gtcggggtgg aacctgcggg gcgggcgggg cgcgggcgcg tgctgctcat
     4321 ggggcgagtc tgccgggacg cgcacacccg gcgcacaccg ggtcagcctg tcacgatgcc
     4381 ggccaccagg ttgatcgcgg tggcgaggat gctggcgccg aagacgtacg agaggaggca
     4441 gtggcggagg acgatcgccc ggatcccgga gtccgagacg ttggtgtccg agacctggta
     4501 cgtcatgccg aggttgtagg agaagtacag gaagtcggtg tagcgggggt ggtcgtcgga
     4561 gttgaagtcg acgccgcctt cgggcggcgc gtagtacagg taggcgtacc gggtcgcgta
     4621 catgaggtgg agcgcggccc aggccatgaa gacgccccag agggcggtcg cggcggcggt
     4681 ccggtcgtag ccggagtcga cgaggagcag ggcgacgatg gcgacgagtc cgcagagggc
     4741 cgccgtgacc accgccagtt cctcgacgac cgggcggaac tcctcgcgcc gcaggttgcg
     4801 gcgggtggcg gcggcgtcca tcggccagag cacgatccag ccggtgacga cgaagacggt
     4861 ctcggcggcg gcgatcccgg cgaggatgcc gagcgccgtg tcggcgagcg cgcccgtgac
     4921 ggccccggcg gcggcgccga cggcgcccgc gccggccagc cgggggaccg cgtaccgcca
     4981 cgaccaggcg cgcccgaccg tcacgcgccg ccgaggacga cgacggcctc gtccgtgtac
     5041 gtgaagtagg tgagcgtctc gtggaggtac agctccacgg tgtcggcgtc gtgccgggtg
     5101 tagccgatgg agacatcctg gccgaggcgg agttcgaagt cgccgccgcg cgtggagagc
     5161 acgtagccgc cggtgagggc gggggcccag atcggcggtc cgtcgagcat ccggccgagg
     5221 tgggcggcga tcggatagcc gtggtcggag gtctcgctga cggcccggta ctcgtcggcg
     5281 ccgaggagca gcgcgtacgg gccgtccacg ccggcgagcc gcagggcggt gagggcgcgg
     5341 ctgacggcgt cggggtagtc gcggggttcg gtgggaagcg cgacgaccag gttggaggag
     5401 gcggcgcgca ggccgcgcac gccggcggcc tcgtagccgt cgaagacgac gctgtcctcg
     5461 gcgagggcga gggtgcgggc ggcgtccttg acgggctgcc agtcggagtc cttggcgccg
     5521 cgttcgacgt cgtcgacggc ggagcggtcg acggtgaagg ggacgcggag ctcgacgagc
     5581 ggccgggcct cgcgcagccg ggcgcggacg cccgcggcgg gcgcggcgag gtcggtgagg
     5641 tggccggtgc cgacggcggc gaggccggcg ccgccggggt cggggacgtc cacgacgcgg
     5701 cggccggcga cggtgcggcg gaaggtgcgg cgggcctcct cctcgatctc gtcccaggcg
     5761 gcgggggtga tgggggcgag ttcgcggtgc agattgctgg tcatgacgcc ggtactcctt
     5821 tcaggctccc gatgcccagg ccgacggcgg cggcagcggg gtctacgggt acggctgcgg
     5881 gtgcgactgc ggggacggct tgggcttcgg ccgcgggctc gggggcggtc tcccgcgccg
     5941 cttccggagc cgggaggtcg tcgagcaggt cggcgctcgg gacgaagaag aggccaccgg
     6001 tgacggcggt ggagaagtcg aggatgcggt cggtgttgcc cggcgggtcg ccgaggaaca
     6061 tgttgcggag catctgttcg gtgacggcgg gggtgcgggc gtacccgatg aagtacgtgc
     6121 cgaactcgcc cgcgccgagc cgtccgaacg gcatgttctg gcggacgatc tgccgctcct
     6181 cgccgtcctc gtcctcgacg gtgttgaggg cgacgtgcga gtcggacggc ttgacgtcgt
     6241 cggggagttc gacgttgtcg gccttggagc ggccgatgac ccgctcctgt tcctcggtgg
     6301 agagcgcgtt ccaggcggtg aggtcgtgga ggtacttctg cacgacgacg tagctgccgc
     6361 cgcggaagcc ggggtcctcg tccgcgacga ggacggcgcc ggcggcggac gggcccgtgg
     6421 ggttctcgct cccgtcgacg aagccgagga ggtcgcggtc gtcgaagtag gtgaagccgt
     6481 ggacctcgtc gacgacggtg acggcgtccc cgaagccgtc gaggacgacc cgggccagtt
     6541 cgaagcagag gtcggcgcgg cgggcgcgca ggtggagcag caggtcgccg ggggtggcgg
     6601 gggcgcggtg ccgggggccc tggagggggg tgaaggggtg gagttcgcgg gggcgcggcc
     6661 cgccgacgag ccggtcccag gcgtcggagg cgagaccggc gacgcaggcg agggcgtcgt
     6721 cacgggcgcg gaaggcgacg gagcgggcga gcccggacag gccgccgagc ccttcgcgta
     6781 cgtcggtctc ccggccgggc gcgacggtga gggtgaggaa gagggcggcc ttggcgggcg
     6841 ggccgaggat aagctggggg acgggctggg gggtggggtg cggggaggca ccgggggtgg
     6901 ggtgcggggt gggatcgggg gcgggctggg gggtggtctg gggggcggcc atgcgactcc
     6961 cgtcaaatgg ggcaaatcgg cgtcagcgta tcgaccgccg cgcgggcccg cagcccgtca
     7021 ccgggccccc gcgcggccga atggcgacgg ggtgcggccc cgcatagcct gccgagaggg
     7081 ggtcctacgc gttccctcgc gcccgtgtgc gccggaaagg tggcaggccc atgagcctcg
     7141 accgaaagca gcaggacggc ctggaacccc gggtgggccc cgttcccgac ggggagccgg
     7201 aggtccggcc gtaccaccat cccgccgctg gctggggcgc cgccaagagc gtgacccggt
     7261 tcatggcccg ggagggcgcc ctccccgtgg acgggccccg ggcgatcatg aagatgaacc
     7321 acgagcgcac cgggttcgac tgtccgggct gtgcctggcc cgacgacacc aagggcctcc
     7381 acctggacat ctgcgagaac ggcatcaagc acgtcacctg ggagatgacc cggaagcggg
     7441 tcgggcggga cttcttcgcc gcccactcgg tgacggagct ggccggatgg accgactacg
     7501 cgctggagaa cgagggccgg ctcaccgagc cgatggtgta cgaccccggc accgaccact
     7561 acgtgccgat cggctggaag gacgccttcg aactggtcgg cagcgccctg cgcggcctcg
     7621 acgacccgaa ccaggcgtcg ttctacacct cgggccggct cggcaacgag gcgaccttcc
     7681 tctaccagct gctcgcccgc gagctcggca ccaacaacct ccccgactgc tccaacatgt
     7741 gccacgaggc gagcgggcgg gccctgacgg cgtcgctcgg caccggcaag ggcacggtcg
     7801 acctcgccga ctgggagacc gccgacgcgc tgttcatcct cggcgtcaac gcggcgtcca
     7861 acgcgccccg gatgctcacc gccctcgccg aggcgtacca ccggggcgcg cagatcgtgc
     7921 acgtcaaccc gctggtggag gccgccgcca cgcggacgat catcccgcac gacttcaggg
     7981 acatggcgac cttccgcgcc accaggacgt cgacgctcaa cctccagccg cggatcggcg
     8041 gcgacatggc cctcgtacgg ggcatggcca aggcggtcct cgaacagtcg gcgtccgatc
     8101 cgaaggcgct cgacccggag ttcatcgagc ggcacacggc cggcttcgag gagtaccggg
     8161 cggtgtgcga ggccacgccc tgggaggaga tcgagctcca gtccggactc ccccgcgagg
     8221 agatcctgaa ggcggcccgg gtgtaccggg aggcggaccg ctcgatcgtc agctggtgcc
     8281 tcggcgtcac ccagcacgag cacggcgtcg acaccgtccg cgagatcgtc aacctcctgc
     8341 tgctgcgcgg caacctgggc cgcgagggcg cgggcccgtc ccccgtacgc ggccacagca
     8401 acgtccaggg gaaccgcacc tgcggcatcg accaccgccc gacggcggcc ttcctcgacc
     8461 ggctcggcga ggtctgcggc atcgacccgc cgcgcgccca cggcctggac acggtgcgca
     8521 ccatcgaggc gatgggccgc ggcgacgtca aggtcttcgt cggcatgggc ggcaacttcg
     8581 cgctcgccgc gcccgacacc cccgtcacgt acgaggcgct gcggaactgc gagctgaccg
     8641 tccaggtgag caccaagctc aaccgcagcc atgtggtgca cggccggcgg gcgctgatcc
     8701 tgccgtgcct gggccgcacc gagaaggacc accagcgcaa gggcgaacag gccacctcgg
     8761 tcgaggactc gatgagcatg gtccacctct cgcgcggcat gaagaagccc gcgtctgcgc
     8821 acctcctctc cgagccggcg atcgtcgcgg gcatggcgcg ggccgcgctc ccggacagca
     8881 cgacgccgtg ggcctggtac gtggaggact acgaccggat ccgggacacg atggcccgcg
     8941 tcctggacgg cttcgaggac ttcaaccggc gggtgcggct gccgctcggc ttccgcatca
     9001 agcagcccgc gcgcgagctc gtcttccgca cgccgtcggg gaaggcggag ttctccgccg
     9061 cgccgctgcc ggacgtcgtc cccgagccgg gcacgctcgc gctcggcacg atgcgctcgc
     9121 acgaccagtg gaacaccacg atctactccg acgacgaccg ctaccggggc gtgagcgggc
     9181 tgcgcaccct ggtcttcatg aaccgcgccg acatgcgcga gcggggcatc acggagctcg
     9241 ccccggtgga catcaccgcg accgcccgcg acggcagccg gcgctcggtg cacggctatc
     9301 tggcggtgcc gtacgacatc ccacgcggct gcgcggcggg gtacatgccg gagatgaacg
     9361 tgctgtgcgc cctgggcgac tacagcaccc agagcgacca gccgatcatg aagcacctca
     9421 aggtcgtgat cgagcctacg ggctgactga cctgtgaaca cacgccgtcg accgctcggc
     9481 gaacggcccc cgcctcacga ggaggcgggg gccgttcgcc gttcaggccg cggcggcggc
     9541 cgtctccgcg gccgcggggc cgcccttgcg gatcgtgcag tggagggagt cgtcgatgac
     9601 gtcggcgacg atcgtgtcgc cgggttccgc ctcgccgccg aggaggaggg aggcgatgcg
     9661 gttgtcgagc tcggtctgga tggtgcggcg cagcggccgg gcgccgaatt cgggctggta
     9721 gccgtgggcg acgaggagct tcgtggcggc ctcggtgacc tccagggtca tgccctgggc
     9781 gtggacccgg tgcttgctgc ggtcgaggag gtgctcgacg atctcggtga ggtccttctc
     9841 ggtgaggctg tggaagacga tgatgtcgtc gatgcggttg aggaactcgg gcaggaagcg
     9901 gccccgcagg tcctccatca gctcgtcctt cagctcggcc gcgtcgccct cgtgggcgag
     9961 gatccggtgg gcgccgatgt tggaggtcat gatgacgacg cagtggcgga agtcgacggt
    10021 acggccctgg ccgtccgtca ggcgcccgtc gtcgaggacc tggagcaggg tgctgaagac
    10081 gtcctggtgg gccttctcca cctcgtcgaa gaggacgacg ctgtacggct ggcggcggac
    10141 cttctcggtg agctggccgg cctcctcgta gccgacgtat ccgggcgggg cgccgacgag
    10201 ccgggcgacg gtgtgcttct cctggaactc gctcatgtcg aagcggatca tgcggtcctc
    10261 gtcgccgaag agcagctcgg cgagggtctt ggcgagttcc gtcttgccga cgccggtggg
    10321 cccgaggaag aggaaggagc cgacgggccg gtcggggtcg cccatgccgg cgcggttgcg
    10381 gcggacggcc tcggagacgg cggtgacggc ctcgtcctgg ccgacgatcc ggtcgtgcat
    10441 ctcctgctcc agcttgagga ggcgctcctt ctcggtggtg gtgagctggc tgacggggat
    10501 gccggtgcgg cgggagacga tgtcggcgat gtcggaggcg gtgacggaga cgacgccctc
    10561 gcggcgctcc tcgatgccgg cgagctcgcc ctcgacctcg gcgatctcct ccttgagctg
    10621 cttggccttc tcgaactcct cggcggcgac ggcctggtcc ttctcccggc gcagcttggc
    10681 gagccggtcc tcgcggctga tcacctcggt ggagcgcccc gcgctgcgga gccgtacgcg
    10741 ggcgcctgcc tggtccatga ggtcgatggc cttgtcgggc aggaagcggt cgctgatgta
    10801 acgg
//
