LOCUS       NZ_JABBGE010000015     10933 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig15, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000015
VERSION     NZ_JABBGE010000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 148941)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 148941)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2371
            Orig. end                         :: 13304
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10933
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10933
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10933
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5933](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5933
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(148..161)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.72"
                     /tool="antismash"
     gene            complement(471..1136)
                     /locus_tag="HHL19_RS36920"
     CDS             complement(471..1136)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="HHL19_RS36920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_206325969.1"
                     /transl_table=11
                     /translation="MRTRQQLLSAAAVEFDLHGYEGTSFSRLSRRAGISVGAVTFHFEA
                     KADLAAAVVQRGTALTRGVAGDAARRGGRAVDAASVLLLSLGALVEREAAVRAAIRLGQ
                     EGGRPGPSWCGAWLPSFQALLEQAAARGELAPGVEPRPVAGLACHLLSGTAWRVRRRPT
                     ALPGRALEELRELWRLVCRGVVAEPYAEPHAAGPHGELRATVPYGGGRAPSVPPQAGP"
     misc_feature    complement(765..767)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1139..1154
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.99"
                     /tool="antismash"
     misc_feature    complement(1409..1418)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    1413..1426
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.02"
                     /tool="antismash"
     misc_feature    1425..1437
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            1513..2709
                     /locus_tag="HHL19_RS33930"
                     /old_locus_tag="HHL19_33940"
     CDS             1513..2709
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641412.1"
                     /locus_tag="HHL19_RS33930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33940"
                     /product="transposase"
                     /protein_id="WP_240982047.1"
                     /transl_table=11
                     /translation="MTALTLPLAPRAATERPHKELPQPTPEPTPEPTSRPTTRPTPLPT
                     DDLALTGFTERLFGHLSRADQRRWARVYLQGLLTAPGKKSVRELAASVTDSPTAYQSLQ
                     QFINASPWEWEPARRELLRWVGERHTPAAWTVGQAVLPKRGSHSVGVHRRFDPAAGRTL
                     NCQLGLGIFACLDETTGIPVDWRLLLPEAWVTDADRRRRARIDPATSYRSPASLILDLA
                     DAVRDAPRAPLVADLSRTARAGELVAALASRGWDLLVAVPPTLTLTPAGLPTGTELTAA
                     GVATLGRLRHPQAMRVHSTLVRLPDLPQPCRLFSQRRNGPFWLTTLTRHRMDDLLHLTL
                     HQGAPAATTRRLADAYGLLDFEGRSYPGWHHHMTLVSAAYAYDVAPVPVRRHRLPGAA"
     misc_feature    1573..1575
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2747..4129)
                     /locus_tag="HHL19_RS33935"
                     /old_locus_tag="HHL19_33945"
     CDS             complement(2747..4129)
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238342.1"
                     /locus_tag="HHL19_RS33935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33945"
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="WP_346768558.1"
                     /transl_table=11
                     /translation="MSKVLPSPAVSLAAIVKAYDVRGIVPDQWDEEVAGLFGAAFVRVT
                     EADAIVVGHDMRPSSPGLSDAFARGAARLGADVTLIGLCSTDQLYFASGSLGLPGAMFT
                     ASHNPARYNGIKMCRAGAAPVGQDTGLSDIRALAERWLVEGAPEAAATPGTVTERDTLA
                     DYAAHLKALVDLTAIRPLKVVVDAGNGMGGHTVPTVFDGLPLDTVPMYFELDGTFPNHE
                     ANPLDPKNIVDLQARVRAEGADLGLAFDGDADRCFVVDERGEPVSPSAITALVAARELA
                     KHPGGTVIHNLITSWSVPEVVRENGGVPVRTRVGHSFIKEEMAKTGAVFGGEHSAHYYF
                     KDFWNADTGMLAALHVLAALGGQEGPLSELVASYDRYAGSGEINSTVADQTAKAAEIKA
                     AYEGEDGVVLDELDGLTVTSADWWFNLRASNTEPLLRLNVEARDAVIMGKVRDEVLALL
                     RA"
     gene            complement(4222..4899)
                     /locus_tag="HHL19_RS33940"
                     /old_locus_tag="HHL19_33950"
     CDS             complement(4222..4899)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138932.1"
                     /locus_tag="HHL19_RS33940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33950"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_169094244.1"
                     /transl_table=11
                     /translation="MADRNRQRTPQPRAVQTRAVILRAAAEVFDEFGFSGASISKIMKR
                     AGVTQGGMYFHFGSKEELAYSVMVSQGEGLDFPKGEDGLQRLIDITLYLAVELRRNPLL
                     RAGVRLAVEQGEFGLRDDVAYQAWVLEFRQQLRFARARGELLPGLDDEDFAWVLVSSFT
                     GAQLFSQVSSERRDLPQRVVSLWRYLLPAVAVPEIRDSLRFDLPDAGAELGVDEGVQGV
                     EVP"
     misc_feature    4913..4926
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(4920..4929)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            5001..5933
                     /locus_tag="HHL19_RS33945"
                     /old_locus_tag="HHL19_33955"
     CDS             5001..5933
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644108.1"
                     /locus_tag="HHL19_RS33945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33955"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_169094245.1"
                     /sec_met_domain="AfsA (E-value: 2.1e-24, bitscore: 78.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPYVDHTATVNHAGLSSALPREYVHKSAHSEVLLTGWKTEGPDT
                     FTVTAQWPRTHAFYAPTDGRHDPLLLAETVRQAVPLLSHVAYDVPFGHRQIWNTFSFTL
                     DPDALAVGATPAEVDMHVRCTDVTRRARRLHGLTMHVELTRDGTPLGTAQAAFTNQPPH
                     LYQRLRGPYADTARALSTALPAPSPLPPHHVDRDRRTDVVLAPTPAPHRTQLRIDPTHP
                     VLFDHPVDHAPGMLLIEAARQAAHHTTSDGHPTAVLTGLHTTFHRYIELDAPCYITTLP
                     TPTRTVEIQATQNDHLSFTAAATLTPRTP"
     misc_feature    5058..5060
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     tRNA            6002..6074
                     /anticodon=(pos:8406..8408,aa:Arg,seq:cct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HHL19_RS33950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HHL19_33960"
                     /product="tRNA-Arg"
     gene            6002..6074
                     /locus_tag="HHL19_RS33950"
                     /old_locus_tag="HHL19_33960"
     gene            complement(6146..7141)
                     /locus_tag="HHL19_RS33955"
                     /old_locus_tag="HHL19_33965"
     CDS             complement(6146..7141)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /locus_tag="HHL19_RS33955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33965"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_346768566.1"
                     /transl_table=11
                     /translation="MTTIEAEIVRGTYQAPDAGKIAFGPYADDWLKHRKLEDRSRERNE
                     GVLRLHIKPTFGTGTLAAITTPRVRAWRTALLEAGVGEPTVVKAYQLLRAILNTAVDDE
                     LIRRNPCRIKGADHYDVPERPVLTVAEVYAVADAIQARYRALVLLAAFTTLRFGELASL
                     RRRDLDLSRCVVLVRRAQSELQNGKLADKAPKSAAGVRPVAFPAELVPELTHHLECFAA
                     AGQDGHLFQGPRGGLLRRSNFRDDWTAARAEAGVSGDVHFHDLRHTGNTLASSAGASTR
                     ELMTRMGHSTTRAALIYQHMTSDRDQHIAGKLGEMIRRSRGEGDSPPSGT"
     gene            complement(7138..7290)
                     /locus_tag="HHL19_RS38630"
     CDS             complement(7138..7290)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHL19_RS38630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_346768569.1"
                     /transl_table=11
                     /translation="MANRKGRRRRFGTVRQLASGRWQARYRDPLSGETKAAPQTFATKT
                     DADVG"
     gene            complement(7290..7493)
                     /locus_tag="HHL19_RS33960"
                     /old_locus_tag="HHL19_33970"
     CDS             complement(7290..7493)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035194.1"
                     /locus_tag="HHL19_RS33960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33970"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_169094246.1"
                     /transl_table=11
                     /translation="MSVRLLTVDQVAELLGTSVRFPRRLIEERRITFVKVGRHVRIPEP
                     AVQAYIETHTVQPIVRRFGKVA"
     gene            complement(7496..8899)
                     /gene="repSA"
                     /locus_tag="HHL19_RS33965"
                     /old_locus_tag="HHL19_33975"
     CDS             complement(7496..8899)
                     /codon_start=1
                     /gene="repSA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035195.1"
                     /locus_tag="HHL19_RS33965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33975"
                     /product="replication initiator protein RepSA"
                     /protein_id="WP_169094247.1"
                     /transl_table=11
                     /translation="MTDTALAAGLDPATLSDLLRVAGSPGFDRLTEQLRRTGGCTAPIR
                     LTGGTKLLDPATRTVIHAYTTDAEPGGILRVACGNRRASRCPACAWTYAGDTYHLIRAG
                     LVGDLGKGTPETIREHPRVFATLTAPSFGPVHNRPGTRPCRCGTRHPEDAPALGTPLDP
                     DTYDYTGAVLWNNHASELWRYFTIYLRREIAARAGLTQKAAREQSRVSFGKVAEYQKRG
                     AVHFHAVIRFDGPDGPDTPPPHWATLDLLTDAIHAAAARVRVVVDAAPAHGIGQDLTLT
                     WGTQLDVQPIGAFGQGEELTEQAVAAYVAKYATKAAETTGTVDHPVGNKEALVLLGVPD
                     HPRRLMEACMDLDTAYPDRRLRAWAHMLGFRGHFSTKSRAYSTTLGALRQVRANYRAQQ
                     QRTALGLPDPDEHPESTLLVVAHWAYAGHGRTPGESWLAANIRRDIQHNREHAKEIRTE
                     VEATPEGEW"
     misc_feature    complement(7736..7738)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8905..9084)
                     /locus_tag="HHL19_RS33970"
                     /old_locus_tag="HHL19_33980"
     CDS             complement(8905..9084)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHL19_RS33970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HHL19_33980"
                     /product="hypothetical protein"
                     /protein_id="WP_169094239.1"
                     /transl_table=11
                     /translation="MMPQTPPARVCSHCDGFPVVHITTGTTQDGQRRTLAATCPACKGT
                     GTRTAPTALARAGK"
     gene            complement(9176..9724)
                     /locus_tag="HHL19_RS33975"
                     /old_locus_tag="HHL19_33985"
     CDS             complement(9176..9724)
                     /EC_number="2.7.7.65"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488567.1"
                     /locus_tag="HHL19_RS33975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33985"
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_169094248.1"
                     /transl_table=11
                     /translation="MDTQTIAAAGLPALGWALHGGLLWRRLATARRDPLTGLHTRAGWT
                     ARADHLMSKHPNALVVLVDLDDFKAINDTHGHAAGDTVLTTTADRLADWCGRHGIAARL
                     GGDEFAAIVTDPARTPGLAELRAALDEPITHRGHALPVSASVGHCHRDQLPVPVLTDAL
                     SAADASMYAVKGHGRRNTR"
     misc_feature    complement(9692..9694)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9730..10047)
                     /locus_tag="HHL19_RS33980"
                     /old_locus_tag="HHL19_33990"
     CDS             complement(9730..10047)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035197.1"
                     /locus_tag="HHL19_RS33980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33990"
                     /product="SpdD-like protein"
                     /protein_id="WP_169094249.1"
                     /transl_table=11
                     /translation="MLQPRIPVNPLPPVHPIPPTRTEPVTTPTPIVVPAPAPAPSTTPR
                     GSVQLTPGSAVALAAGGGAVVLVVGAVLVSMLLAVAISAASIAVVAVVLRLLVRDMQKG
                     R"
     gene            complement(10060..10215)
                     /locus_tag="HHL19_RS33985"
                     /old_locus_tag="HHL19_33995"
     CDS             complement(10060..10215)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134826.1"
                     /locus_tag="HHL19_RS33985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33995"
                     /product="hypothetical protein"
                     /protein_id="WP_169094250.1"
                     /transl_table=11
                     /translation="MDVPLWLALIVVGGLGIKLIRPPWWLVAALLLGGYLLADSLLTPV
                     IDPIAK"
     gene            complement(10235..10423)
                     /locus_tag="HHL19_RS33990"
                     /old_locus_tag="HHL19_34000"
     CDS             complement(10235..10423)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177726.1"
                     /locus_tag="HHL19_RS33990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_34000"
                     /product="mobile element transfer protein"
                     /protein_id="WP_169094251.1"
                     /transl_table=11
                     /translation="MRPNRFHNVIKIGPVQVGTHRDGRGRTKHTAVCTAPGCDFSADYN
                     TRAAAELAARTHRCQPR"
ORIGIN
        1 cggcatgccg gcggcgaccg cctcctcgac gacgtactgg atggcgggct tgtcgacgac
       61 cggcagcatc tccttcggcg tggccttggt gaccggcagg aaccgggtgc cgagaccggc
      121 cgcgggaatg accgccttgg tgatggggga aggtgtgttc gacatggtgg cgaggctagg
      181 gaatcaggcc cggaaaaagc acttcccgcc gttcccccgg accccacttc ggcatttcgt
      241 caaagtcgcc ccggccgccg tgagtcgaat tccgtaaatg cggtacgagg cattccgggc
      301 attccgatgg cgtttcccgc gctcatacgc aaagccccga ccgcgataga gcgacggccg
      361 gggcggggta cgggtcgggg gtacgggtcg gggtatgggg cgggcgggcg aggcccaggg
      421 aggcaggggg tccgggggcc agggggtccg gggggccagg gggcaggggg tcagggaccg
      481 gcctgcggcg ggacggacgg cgcccgaccg ccgccgtacg ggacggtggc ccggagctcg
      541 ccgtgcgggc cggctgcgtg gggttccgcg tacggctccg cgaccacccc ccggcagacg
      601 agccgccaca gctcgcgcag ctcctccagc gcgcggccgg gcagggcggt gggccgccgc
      661 cggacgcgcc aggcggtgcc ggacagcagg tgacaggcga gcccggcgac cgggcggggc
      721 tcgacgcccg gcgccagctc ccccctcgcg gcggcctgtt cgagtaacgc ctggaaggac
      781 gggagccagg cgccgcacca cgacggcccc ggccgcccgc cctcctggcc gagccggatc
      841 gcggcccgga cggccgcctc gcgctcgacg agcgcgccga gggagagcag caggacggag
      901 gcggcgtcga ccgcccggcc gccgcgccgc gcggcatcgc cggcgacgcc ccgggtcagg
      961 gccgttccgc gctgcaccac ggcggcggcg agatcggcct tcgcctcgaa atggaaggtg
     1021 accgcaccca cggaaatgcc cgcgcgccga ctcaaccggg agaacgaggt cccctcgtaa
     1081 ccgtgcaggt cgaattcgac cgcggccgcg gagaggagct gctggcgggt gcgcacggcg
     1141 cgcgtttgtt tcaccacgta ttcatcactg cttcatcact gccattcgga tcgtcactgc
     1201 cccgccgata cgcgtcgata cacgcgtcga ccacgtcggc gcgcgtcgtc gcgcgccgtc
     1261 cccgaagggt cagcggcacc gcagcctgcc cgcacgcgca ccccgacgcg agggctactt
     1321 ggcgaagacg tcacccaccc cgggtgctcc gccgggcgcc ctcccccaac ctgcccaagt
     1381 cgcccctcga agaccagagg aacactcgaa aaaaaacgat cgttctgtat tctgcaagga
     1441 cggatcgatc gacggaccga tggatcccgc ccggccgacg cacccgtcgg ccgttaccga
     1501 ggaggcggag agatgacggc actgaccctg cccctggctc ccagagcagc cacggagagg
     1561 ccgcacaagg agttacccca gcccacgccc gagcccacgc ccgagcccac gtcccggccc
     1621 acgacccggc ccacgccact acccaccgac gacctggccc tgaccggctt caccgagcgg
     1681 ctcttcggcc atctgtcccg cgccgaccag cggcgctggg cgcgggtcta cctccagggg
     1741 ctgctgaccg cacccggcaa gaagtccgta cgggagctcg ccgcctcggt cacggactcc
     1801 cccaccgcgt accagtcgct ccagcagttc atcaacgcga gcccctggga gtgggagccc
     1861 gcccgccgcg agctcctgcg ctgggtcggc gagcgccaca cccccgccgc ctggaccgtg
     1921 gggcaggcgg tcctgcccaa gcgcggctcc cactccgtcg gcgtgcaccg ccggttcgac
     1981 ccggccgccg gacggaccct caactgccag ctcggcctcg ggatcttcgc ctgcctcgac
     2041 gagaccaccg gcatcccggt cgactggcgg ctgctgctcc cggaggcctg ggtcaccgac
     2101 gccgaccggc gccgccgcgc ccggatagac cccgcgacct cctaccgctc ccccgcctcg
     2161 ctgatcctgg acctcgcgga cgccgtccgg gacgcgcccc gcgcacccct ggtcgcggac
     2221 ctgagccgga ccgcgcgggc gggagagctg gtcgccgcgc tggcgtcccg cggctgggac
     2281 ctgctcgtcg ccgtcccgcc gacgctcacg ctcacccccg ccggcctccc caccggcacc
     2341 gaactcacgg ccgccggcgt cgccaccctc ggccggctac gccaccccca ggcgatgcgg
     2401 gtccactcga ccctcgtccg cctccccgac cttccgcagc cctgccgtct gttcagccag
     2461 cgccgcaacg gccccttctg gctcacgacc ctcacccgcc accggatgga cgacctcctc
     2521 cacctgaccc tccaccaggg cgcacccgcc gcgacgaccc gccgcctcgc cgacgcgtac
     2581 ggcctcctgg acttcgaggg gcgctcgtac cccggctggc accaccacat gacgctggtc
     2641 tcggcggcgt acgcctacga cgtcgccccc gtgcccgtgc gccgccaccg cctccccggc
     2701 gcggcatgac cgccacggcc gggaaggcgc gccgggagct ccgggatcag gcccggagca
     2761 gggcgaggac ctcgtcgcgg accttgccca tgatcacggc gtcccgcgcc tcgacgttga
     2821 ggcggaggag gggttcggtg ttggaggcgc ggaggttgaa ccaccagtcg gccgaggtga
     2881 cggtgagacc gtcgagctcg tcgaggacga cgccgtcctc gccctcgtac gcggccttga
     2941 tctcggcggc cttggcggtc tggtcggcga cggtggagtt gatctcgccg gagccggcgt
     3001 accggtcgta cgaggcgacc agctccgaca gcggcccctc ctggcctccg agggcggcga
     3061 gcacgtggag ggcggcgagc atgcccgtat cggcgttcca gaagtccttg aagtagtagt
     3121 gcgccgagtg ctcgccgccg aagaccgccc cggtcttcgc catctcctcc ttgatgaagg
     3181 agtggccgac gcgggtgcgg acgggcacgc cgccgttctc gcggacgacc tcggggaccg
     3241 accaggaggt gatcaggttg tggatgactg tcccaccggg atgcttggcg agttcgcggg
     3301 cggcgacgag ggcggtgatc gcggaggggg agacgggctc gcctcgctcg tcgacgacga
     3361 agcaccggtc ggcgtccccg tcgaaggcga gaccgaggtc ggcaccctcc gcccggacac
     3421 gggcctggag gtcgacgatg ttcttcgggt cgagggggtt cgcctcgtgg ttcgggaagg
     3481 tgccgtcgag ctcgaagtac atcggaacgg tgtccagcgg gaggccgtcg aagacggtgg
     3541 ggacggtgtg gccgcccatg ccgttgccgg cgtcgacgac gaccttgagg gggcggatcg
     3601 cggtgaggtc gacgagcgcc ttgaggtggg cggcgtagtc ggcgagggtg tcccgctcgg
     3661 tgacggtccc gggcgtggcg gcggcctcgg gggcaccctc cacgagccag cgctccgcga
     3721 gggcgcggat gtcggagagg ccggtgtcct gcccgacggg cgcggcgccg gcacggcaca
     3781 tcttgatgcc gttgtaccgg gccggattgt gcgaggcggt gaacatcgca ccgggcagac
     3841 ccagactccc ggaggcgaag tacaactgat cggtggaaca cagcccgatg agggtgacgt
     3901 cggcgccgag gcgggcggca ccgcgggcga aggcgtcgga gaggccgggc gaggaggggc
     3961 gcatgtcgtg gccgacgacg atggcgtcgg cctcggtgac gcggacgaag gcggcgccga
     4021 agagcccggc gacctcctcg tcccactggt cgggcacgat cccgcggacg tcgtacgcct
     4081 tcacgatcgc ggccagcgag acagcggggg acggcaagac cttcgacacg tcgagctcct
     4141 tcaggcggag gggagggacg ccgccggacg ctacccgcgc ttccatccca cccgcccgca
     4201 gaccgccgat tcacacgacg gctatggcac ctcgacgccc tgaacgccct cgtccacccc
     4261 caactccgcc cccgcatcag gcagatcgaa ccgcagcgag tcccggatct cgggcacggc
     4321 cactgcgggc agcaggtagc gccacaggga gacgacccgc tggggcaggt cgcggcgctc
     4381 ggacgacacc tgggagaaca gctgcgcacc ggtgaaggag ctcacgagca cccaggcgaa
     4441 gtcctcgtcg tcgagcccgg gcaggagctc gcccctggcg cgggcgaacc gcagttgctg
     4501 ccggaactcc agcacccagg cctggtacgc cacatcgtca cgcagcccga actcgccctg
     4561 ctcgacggcg agtcggaccc cggcgcggag cagcggattg cgccgcagct cgacggccag
     4621 gtacagggtg atgtcgatga gccgctgaag cccgtcctcc cccttgggga agtcgaggcc
     4681 ctctccctgg ctgaccatca cggagtaggc gagctcctcc ttggagccga agtggaagta
     4741 catgcccccc tgggtgacgc cggcccgctt catgatcttg ctgatgctgg ccccgctgaa
     4801 cccgaactcg tcgaacacct cggccgcggc gcgcaggatc accgccctgg tctgcaccgc
     4861 cctcggttgc ggcgtcctct gtcggttccg atctgccacc gtcccgctcc cctctcgaca
     4921 aaaaaaacga acatcctcta ttttagagcg tgtgcccggt cagggccatc cacactgcgc
     4981 acgacacctg ggggaatccg atgactccat acgtcgacca caccgccacc gtgaaccacg
     5041 cgggtctgag cagcgcgtta ccgagggagt acgtgcacaa gagcgcgcac tccgaagtcc
     5101 tcctcaccgg ctggaagacg gaaggtcccg acaccttcac cgtcaccgcc cagtggcccc
     5161 gcacccacgc gttctacgcg cccaccgacg gccgccacga cccgctgctg ctcgccgaga
     5221 ccgtccgcca ggccgtcccg ctgctgagcc acgtcgccta cgacgtgccc ttcggccacc
     5281 gccagatctg gaacaccttc tccttcacgc tcgaccccga cgccctcgcc gtcggcgcca
     5341 ccccggccga ggtcgacatg cacgtccggt gcaccgacgt cacccgccgc gcccgccgcc
     5401 tgcacggcct cacgatgcac gtcgagctca cccgcgacgg cacccccctc ggcaccgccc
     5461 aggccgcctt caccaaccag ccgccccacc tctaccagcg gctccgcggc ccgtacgccg
     5521 acaccgcccg cgccctctcc acggccctcc ccgcgccgtc cccactgcct ccccaccacg
     5581 tggaccgcga ccgccgcacc gacgtcgtcc tggcccccac cccggccccc caccggaccc
     5641 agctgcgcat cgaccccacg cacccggtgc tcttcgacca ccccgtcgac cacgcccccg
     5701 gcatgctcct catcgaagcc gcccgccagg ccgcccacca caccacctcc gacggccacc
     5761 ccaccgccgt cctcaccggc ctccacacca cgttccaccg ctacatcgag ctggacgccc
     5821 cctgctacat cacgaccctc cccaccccca cccgcacggt cgagatccag gccacccaga
     5881 acgaccacct ctccttcacc gcggccgcca ccctcacccc ccgcaccccg taatcacccc
     5941 cacacacaaa ccgaggcgcc cccaccaccc cccatccgct aggctgtgac ccgccatccc
     6001 cgcctccgta gctcagggga tagagcaccg ctctcctaaa gcgggtgtcg caggttcgaa
     6061 tcctgccggg ggcacaagcc gaagggccag ctcacagggg tttttccccg gagctggccc
     6121 tttcgcatga tcacgcccgt gccacctacg tgccagaagg cggcgaatca ccctccccac
     6181 gggaccggcg gatcatctcc ccgagcttcc ccgcgatgtg ctgatcacgg tcgctcgtca
     6241 tgtgctgata gatcagcgcg gcccgggtcg tgctgtgccc catccgcgtc atcagctcac
     6301 gcgtgctcgc gccggcgctg gaggcgagcg tgttcccggt gtggcgaaga tcgtgaaagt
     6361 gcacgtcacc ggagacgccg gcctctgcgc gcgcggccgt ccagtcgtcg cggaagttgc
     6421 tccgccgaag cagaccgcca cggggcccct ggaagaggtg accgtcctgc ccggccgcgg
     6481 cgaagcactc caggtggtga gtcagctcgg ggacgagttc agcggggaag gcgaccggcc
     6541 ggacgccggc ggcggacttc ggcgccttgt cggcgagctt cccgttctga agctccgact
     6601 gggcccggcg gacgaggacc acacagcggc tcaggtcgag gtcccggcgt cgcagcgagg
     6661 ccagctcacc gaaccggagc gtggtgaagg ccgcgaggag gacgagcgcc cggtatcgag
     6721 cctggatggc gtcggccacg gcgtacacct cagcgaccgt gaggacggga cgctctggca
     6781 cgtcgtaatg atcggctccc ttgatgcggc acgggttgcg gcggatcagc tcgtcgtcga
     6841 ccgccgtgtt gaggatcgct ctcaggagct ggtacgcctt gaccacggtg ggctcgccca
     6901 cgccggcttc cagcagggcg gtacgccagg cccggacgcg gggtgtggtg atggcggcaa
     6961 gggttccggt gccgaaggtc ggcttgatgt gcaaccggag cacgccctcg ttccgctccc
     7021 gggaacggtc ctccagcttg cggtgcttca gccagtcatc ggcgtacggg ccgaaggcga
     7081 tcttgcccgc gtcgggtgcc tgataagtgc cccggacgat ctccgcctcg atggtggtca
     7141 gcccacatcc gcgtcggtct tcgtcgcgaa ggtctgcggc gcggccttcg tctcgccgga
     7201 cagagggtcg cgatagcggg cctgccaacg gccagacgcg agctgccgca cggtcccgaa
     7261 ccggcggcgc cggcctttgc ggttcgccat cacgccacct tcccgaaacg ccggacgatg
     7321 ggctgcacgg tgtgggtctc gatgtacgcc tgcaccgcgg gctccgggat gcggacgtgc
     7381 cgcccgacct tgacgaaggt gatgcgccgt tcctcgatca ggcgccgagg gaaccggacg
     7441 gaggtgccga ggagttcggc gacctggtcc acggtgagca gacggacgga catggtcacc
     7501 actccccttc aggggttgct tcgacttcgg ttcggatctc tttcgcgtgt tcgcggttgt
     7561 gctggatgtc gcggcggatg ttggcggcga gccaggattc gccgggggtg cggccgtggc
     7621 cggcgtaggc ccagtgggcg acgacgagga gggtggactc cgggtgctcg tcggggtcgg
     7681 gcaggccgag ggcggtgcgt tgctgctgtg cgcggtagtt ggcgcggacc tggcgtaagg
     7741 cgccgagggt ggtggagtag gcgcgggact tggtggagaa gtggccgcgg aagccgagca
     7801 tgtgggccca ggcgcggagc cgccggtccg ggtaagcggt gtcgaggtcc atgcaggctt
     7861 ccatcaggcg ccgggggtga tccgggacgc cgaggaggac gagggcttcc ttgttgccga
     7921 ccgggtggtc gacggtgccc gtggtctcgg cggccttggt ggcgtacttc gcgacgtagg
     7981 cggccacggc ctgctcggtc agttcctcac cctggccgaa ggcaccgatg ggctgcacat
     8041 cgagctgggt gccccaggtg agcgtcaggt cctggccgat gccgtgagcg ggggcagcgt
     8101 caacgaccac gcgcacgcgg gcggcggctg cgtggatggc gtcggtgaga aggtcgaggg
     8161 tggcccagtg cggaggcggg gtgtccggtc cgtcggggcc gtcgaagcgg atgacggcgt
     8221 ggaagtggac ggccccgcgc ttctggtact cggcgacctt gccgaaggag acgcgggact
     8281 gttcgcgggc ggctttctgg gtgaggccgg cgcgggcggc gatctcgcga cggaggtaga
     8341 tcgtgaagta gcgccacagc tcggaggcgt ggttgttcca cagcacagcg cccgtgtagt
     8401 cgtacgtgtc cgggtcgagc ggagtgccca gcgcgggggc gtcctcgggg tgacgggtgc
     8461 cgcagcggca ggggcgggtg ccggggcggt tgtggacggg gccgaaggac ggggcggtga
     8521 gggtggcgaa aacccgcggg tgttcccgga tggtctccgg ggtgcccttg ccaaggtcgc
     8581 cgacgaggcc ggcgcggatg aggtggtagg tgtcgccggc gtaggtccag gcgcaggccg
     8641 ggcagcggga ggcgcggcgg ttgccgcagg cgacgcggag gatgccgccg ggttcggcgt
     8701 cggtggtgta ggcgtggatc acggtgcggg tggcggggtc gaggagcttg gtgccgccgg
     8761 tgagacggat gggggcggtg cagccgccgg tgcggcggag ttgttcggtg aggcggtcga
     8821 agccggggga cccggccacc cggagcaggt cgctcagggt cgccgggtcc aggcccgccg
     8881 cgagggcggt gtcggtcaag gccgtcactt cccggcgcgg gcgagggcgg tgggggcggt
     8941 gcgggtgccg gtgcccttgc aggcggggca ggtggcggcg agggtgcggc gctggccgtc
     9001 ctgggtggtg ccggtggtga tgtggactac ggggaagccg tcgcagtggg agcagacgcg
     9061 ggcgggcggg gtctggggca tcatgaggtt tccctttcgg gtccggtgga tctggaatgg
     9121 ggtgaaggcg cccggggcgg cggaagtttg gcgaccgagg ccgccccggg ggccgctagc
     9181 gggtgttgcg cctgccgtgg cccttgaccg cgtacatcga cgcgtcggcg gccgagaggg
     9241 cgtcggtgag gaccgggacc ggcagctggt cgcggtggca gtgcccgacc gaagcggaga
     9301 ccggcagggc gtgcccgcgg tgggtgatcg gctcgtccag cgcggcccgg agctcggcga
     9361 ggccgggggt gcgagcgggg tcggtgacga tggcggcgaa ctcgtctccg ccgaggcggg
     9421 cggcgatgcc gtgacggccg caccagtcag cgagcctgtc ggcggtggtg gtgaggacgg
     9481 tgtcgccggc ggcgtggccg tgggtgtcgt tgatggcctt gaagtcgtcc aggtcgacca
     9541 ggaccacgag cgcgttcggg tgcttgctca tgaggtggtc ggcgcgggcg gtccatccgg
     9601 cgcgggtgtg caggccggtg agcgggtcgc ggcgggcggt ggccaggcgg cgccagagca
     9661 gcccgccgtg cagggcccag cccagagccg gtaagcccgc cgccgcgatc gtctgcgtgt
     9721 ccacggcagc taccgaccct tctgcatgtc gcggaccagg agccggagca cgaccgcgac
     9781 gacggcgatg gacgcggcgc tgatggcgac ggcgaggagc atggagacca ggacggcgcc
     9841 gacgaccagg acgacggcgc caccacccgc ggcgagggcg accgcgctgc cgggggtgag
     9901 ctggaccgag ccgcggggcg tggtggacgg ggcgggggcg ggggccggga ccacgatcgg
     9961 ggtcggggtg gtcaccggtt cggttcgggt cggggggatg gggtggacgg gcgggagcgg
    10021 gttgaccggg atgcggggct ggagcatggc ggggtctccc tacttggcga tggggtcgat
    10081 gaccggggtg aggaggctgt cggcgaggag gtagccgccg aggagaaggg cggcgacgag
    10141 ccaccagggc gggcggatga gtttgattcc gaggccaccg acgacgatca gggcgagcca
    10201 gagcgggacg tccatgggac gggctccttc gggttcagcg gggctggcag cggtgggtgc
    10261 gggcggcgag ttcggcggcg gcgcgggtgt tgtagtcggc ggagaagtcg cagccggggg
    10321 ccgtgcacac ggcggtgtgc ttggtccggc cgcggccgtc gcggtgggtg ccgacctgca
    10381 ccgggccgat cttgatgacg ttgtggaagc ggttgggtcg cacgggacgt acctccttcc
    10441 tgggtcaggc gagctgaagg gcgatggagt cggcgagggc agagggcatg ccgtggcggg
    10501 tgcggagggt ttccgcgtcg atcggccgcc cggtctgagc ccggtgcgcg tccgcgatct
    10561 tctgggcgtg agccatcagc gcgggcggca ccgccacagc tggcaccgca ggaacgggcg
    10621 cgggtccgag ctgttcttcc tgcgcggcag gttccggctg gatccccacc ggttcgggtt
    10681 cggccggcgg aagttcgggc gccggttccg gcgcccgttc cacggtcact tcggccaccg
    10741 gctccgggac cggtgcgggt tccggggccg aggtgggttc ggaggtggga tgggcgtggg
    10801 cgaggagggt gccgccgagg aaggcgatgg cgggccatcc ggcgacgagg atccgcagcc
    10861 aggccgggac gtcgttcatg tcgaggagtc cggcggtggc gacgttggcg ccgagggacg
    10921 cgatgagggc gat
//
