LOCUS       NZ_JABBGE010000014     11008 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig14, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000014
VERSION     NZ_JABBGE010000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 209990)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 209990)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3730
            Orig. end                         :: 14738
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11008
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11008
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11008
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:6008](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6008)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(589..602)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    867..887
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.17"
                     /tool="antismash"
     gene            complement(2394..3377)
                     /locus_tag="HHL19_RS33060"
                     /old_locus_tag="HHL19_33070"
     CDS             complement(2394..3377)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180378.1"
                     /locus_tag="HHL19_RS33060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33070"
                     /product="carbohydrate kinase family protein"
                     /protein_id="WP_169094077.1"
                     /transl_table=11
                     /translation="MRIAVTGSIATDHLMVFPGRFADQLVPDQLAHLSLSFLVDDLEVR
                     RGGVAANIAFGLGSLGLTPQLVGSVGDDFAEYELWLKRHGVDTGAVHVSTTRQTARFMC
                     LTDQDGNQIAAFYAGAMAEAADTGLDGMLSGPARPDLVLVCPNEPAAMLRFTQECRDHG
                     VPFAADPSQQLARLNGEEVRRLVEGAEWLFTNEYEKALVLERLGCTEDDVLDKVGAWVT
                     TLGAAGVRIDRRGEEPLVVPAVPGVRSLDPTGAGDAFRAGFLTALSRGLSERTAARLGC
                     VVAGAALETVGSQAYETDAARLTEVFAAAYGQEAAREVEPSWGARS"
     gene            complement(3426..4649)
                     /gene="metK"
                     /locus_tag="HHL19_RS33065"
                     /old_locus_tag="HHL19_33075"
     CDS             complement(3426..4649)
                     /EC_number="2.5.1.6"
                     /GO_function="GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="metK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019059730.1"
                     /locus_tag="HHL19_RS33065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33075"
                     /product="methionine adenosyltransferase"
                     /protein_id="WP_169094078.1"
                     /transl_table=11
                     /translation="MSRRLFTSESVTEGHPDKIADQISDTILDALLAQDPNSRVAVEAL
                     VTTGQVHVAGEVTTSAYADIATLVREKILDIGYDSSAKGFDGASCGVSVSIGQQSPDIA
                     QGVDTAYEKRVEGASRKDAGDELDKQGAGDQGLMFGYASAETPSLMPLPIDLAHRLSRR
                     LTEVRKEGIVPYLRPDGKTQVTIAYEDDRPVRLDTIVVSSQHAPGIDLDSLLAPDIRLH
                     VVEHVLAQLAADGIKLATDDYRLLVNPTGKFEIGGPMGDAGLTGRKIIVDTYGGYARHG
                     GGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERCEVQVAYAIGKAEPVGLFVETFGT
                     ATVPQKDLERAIGEVFDLRPAAIIRDLDLLRPVYAATAAYGHFGRDDDGFTWERTDRAA
                     QLKAAAGL"
     gene            complement(5001..6008)
                     /locus_tag="HHL19_RS33070"
                     /old_locus_tag="HHL19_33080"
     CDS             complement(5001..6008)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140400.1"
                     /locus_tag="HHL19_RS33070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33080"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_240982031.1"
                     /sec_met_domain="AfsA (E-value: 6.2e-23, bitscore: 74.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPHFAEQSSTARAMGTGRVSVVEQDLTRVPAQRLTSTVPREYVHR
                     AAVSEVFLTGWDAIGPVDPRGGSESFVVRAQWPRSHSLFARDGEFQDPLLLIESVRQVG
                     SLLAHAEFDVPFGHQFLMRDMSVSLEPELLRAGNTPTEVVLRTECRDIVRRNGALSSMG
                     YVVTVERDGRRLGTAEASFRCMSPQVFQRLRGDTPTHSDQPMPPAVEPALVGHTSPRHV
                     VLGVPAPGTAGRWQLRVDTAHPTYFDHPVDHVPGMVLLEAARQAAHAVSGMPDALVLGL
                     RSTFTCYAEFEAPCWIEARTVPGERADGVPVRVEGTQRGKPVFTTDLVVAPRTR"
     misc_feature    complement(5931..5933)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6250..6263
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.59"
                     /tool="antismash"
     gene            6326..7108
                     /locus_tag="HHL19_RS33075"
                     /old_locus_tag="HHL19_33085"
     CDS             6326..7108
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740558.1"
                     /locus_tag="HHL19_RS33075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33085"
                     /product="response regulator transcription factor"
                     /protein_id="WP_169094079.1"
                     /transl_table=11
                     /translation="MNRQAVDLLTSPTEHARPRHPQGGPSRPHFAASPTPHRQEGLRVL
                     VVEANTRDADTLLQGLTRQGYVTQSAKNGAQALRSHSDADLVLLDLDLPDLDGLEVCRG
                     IRTVSETPIITVTARDSELDRVLGLQAGADDYLVKPYGFRELLARMEAIMRRVHRQTTP
                     ARVAVHLGLRVDADARTATLDDEPLDLTRKEFDLLFLLASQPDTVISRRQIMAQVWGDA
                     WSPRGRTIDTHVGTLRGKLGSSEWIHTVRGIGFRLGCP"
     misc_feature    7189..7203
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.2"
                     /tool="antismash"
     misc_feature    complement(7230..7240)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            7235..7861
                     /locus_tag="HHL19_RS33080"
                     /old_locus_tag="HHL19_33090"
     CDS             7235..7861
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546903.1"
                     /locus_tag="HHL19_RS33080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33090"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_169094080.1"
                     /transl_table=11
                     /translation="MAKQERAQRTRQAILTAAAELFDERGYDSASTTDILARAGLTRGA
                     LYHHFPSKEAIALALLDTHIDALQVEEQPVKLQSLIDLTFRFAQQLQSDPALRASVRLS
                     VEQSSFAAFQTGYEQSIEVIRSLLRQAMDQGEMLPGLDIDEATLFIVGAYTGVQTMDQA
                     YSGRAELIDRIGSLWRFVLPGLATPGLISRLRTTPPAGAPRPSST"
     gene            complement(7869..8447)
                     /locus_tag="HHL19_RS33085"
                     /old_locus_tag="HHL19_33095"
     CDS             complement(7869..8447)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740556.1"
                     /locus_tag="HHL19_RS33085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33095"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_240982032.1"
                     /transl_table=11
                     /translation="MAELVADGPASDAGLVNVCQRAGVSRGALYHHFASTAMLAAAVYE
                     EAHHRIVALIDEAFGREPADALRSFLVTLGESLRTDPIVRAGMKLAGDGTFQSPRLRDQ
                     MLAMLHERLVLAGRRAAYPVEDLADLVVLLAAGVETLGRSDPRWWEGRSVDRLWPMLQA
                     LFDSDGGSGDSGGRGVVRARPSVPRPAEG"
     misc_feature    8432..8446
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(8896..8911)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.33"
                     /tool="antismash"
     misc_feature    8897..8910
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.68"
                     /tool="antismash"
     gene            9022..9942
                     /locus_tag="HHL19_RS33090"
                     /old_locus_tag="HHL19_33100"
     CDS             9022..9942
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522983.1"
                     /locus_tag="HHL19_RS33090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_33100"
                     /product="potassium channel family protein"
                     /protein_id="WP_169094082.1"
                     /transl_table=11
                     /translation="MRGPFRIILWRLALAFGLIALAAAVVFLGRSGYRDSAGHPVGRLT
                     SFSYAAVTLTTIGYGDVVPVSGTARLVNTLVITPLRLGFLLILVGTTISALTERTRAEW
                     REQSWRRKVRGHTVVVGYGTKGRAAVVTLRARGTRPREIVVVDVSAAAVDEANRHGLVG
                     IVGDGTRTDVLERAELGRAGHVLIATTRDDTAVLAALTARRMNGAAVITSAETAGHLLG
                     VASLSPAVGTVLEELLSTGLGLEIAERPPAPAEVGHAPGQIAEPVLAVVRDGSPLRYND
                     PRIGAIRPDDRLVTAREPRRGPSPD"
     misc_feature    complement(10982..10997)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.16"
                     /tool="antismash"
ORIGIN
        1 ctcctccagg ttctccttca tgatcacggt ggacttcacg aggagccccg acatgccgat
       61 gacgtcggcc ttgtgctcct ctgccgcctc caggatcgcg gagaccggct gcttgatgcc
      121 gatgttgacg acgtcgtagc cgttgttgga gaggatgatg tcgacgaggt tcttgccgat
      181 gtcgtggacg tcgccgcgca cggtggcgag cacgatcgtg cccttgccgt ccgcgtcgga
      241 cttctccatg tgcggctcca ggtgggccac cgccgtcttc atcacctcgg ccgactggag
      301 cacgaacggc agctgcatct tgcccgcgcc gaacagttcg ccgaccgtct tcatcccgtc
      361 gagcagcacc tcgttgacga tctccagcgc cggccgcacg ccgagcgcct cgtcgaggtc
      421 ggcctccagg ccgttcttct cgccgtcgat gatccgccgc ttgagccgct cctccagcgg
      481 cagcgccgcc agctcctccg ccttgcccgc cttcagggac ttggccgtgg caccctcgaa
      541 gagcgccatc agcttctgca gcgggtcgta accctcggca cgccggtcgt agatcagatc
      601 cagcgcggtg gtgacctgct cctcgtcgaa acgggcgatc ggcaggatct tcgaggcgtg
      661 cacgatcgcc gagtccagac ccgccttcac acactcgtcg aggaagaccg agttcagcag
      721 gatccgcgcg gccggattga gaccgaagga gatgttcgac agaccgagcg tcgtctgcac
      781 ctccgggtgg cgccgcttca gctctcggat cgcctcgatc gtcgcgacac cgtcgccccg
      841 tgactcctcc tggccggtac agatggtgaa ggtcagggtg tcgatgagga gatccgactc
      901 gtggaacccc cacttctccg tcaggtcggc gatgattcgc tcggcgatct ccaccttctt
      961 ctcgacggta cgggcctggc cctcctcgtc gatggtgagg gcgatcagcg ccgcgccgtg
     1021 ctcctgggcg agccgggtga cctgcacgaa gcgggactcg gggccgtcgc cgtcctcgta
     1081 gttgacggag ttgatgaccg cacggccacc gagcttctcc agacccgccc gcagcaccgg
     1141 cacttcggtg gagtcgagca cgatcgggag ggtcgaggcg gtcgcgaacc ggcccgccag
     1201 ctcctccatg tcggcgacgc cgtcacggcc gacgtagtcc acgcacagat cgagcatgtg
     1261 cgcgccctcg cggatctggt cccgggccat ctccacgcag tcgtcccacc ggccctccag
     1321 catggcctcg cggaacttct tcgacccgtt ggcgttcgtc cgctcgccga tggcgaggta
     1381 cgaggtgtcc tgccggaacg ggacatggct gtacagggac gccgcgcccg gttccggccg
     1441 gggctcccgt gccgcgggct ccctcccccg tacgcgctcc acgatcgccc gcaggtgatc
     1501 cggagtcgta ccgcagcagc cgcccaccag cgagaggccg tactcgcgca cgaactgctc
     1561 ctgcgcgtcg gccagttcga ccgggcccag cgggtagtgg gcgccctcgg aggtcagtac
     1621 cggcagcccc gcgttcggca tcaccgacag cgggatccgg gagtgccggg acaggtagcg
     1681 caggtgctcg gccatctcgg ccgggccggt ggcgcagttc aggccgatca tgtcgatgcc
     1741 cagcggctcc agggccgtca gggccgcccc gatctcggag ccgaggagca tggtgccggt
     1801 ggcctccacc gtgaccgaga cgatcagcgg cacgtcggcg ccgagcgccg ccagggcgcg
     1861 gcgggcgccg atgacggacg ccttcgtctg gagcaggtcc tgcgtggtct cgacgatcag
     1921 cgcgtcggcg ccgccggtga ggaggccctc ggcgttctgc tggtaggcgt cgcgcagcac
     1981 cgtgtacggg gcgtgcccga gggtcggcaa cttggtgccg ggaccgatgg agcccaggac
     2041 ccagcgctgc cggccgtcga cggccgtgaa ctcgtccgcg acctcacggg cgacccgggc
     2101 cccggccacc gacagctcgt ggacgcggtg ggcgatgtcg tactccccca gcgccgcgtg
     2161 attggcaccg aaggtgttcg tctccacgca gtcgacaccc gcggcgaagt actcacggtg
     2221 caccgaggcc acgatgtccg gccgcgtgag attgaggacc tcgttgcagc cttcgaggtt
     2281 ctcgaaatcc tggaggctgg gttcctgggc ctggagcatc gtgcccatgg cgccgtcggc
     2341 gacgacgacc cgctcggcga gtgcctcgcg gagggcggtc gcgcggctcg cggtcatgac
     2401 cgcgctcccc acgacggttc gacctcccgc gcggcctcct ggccgtacgc ggcggcgaac
     2461 acctccgtca gccgggccgc gtcggtctcg tacgcctgcg agccgaccgt ctccagggcc
     2521 gcgcccgcca ccacgcagcc cagcctggcg gccgtccgtt cggacaggcc gcggctgagc
     2581 gcggtgagga acccggcccg gaaggcgtcg cccgcgccgg tcggatccag cgaacgcacc
     2641 ccggggacgg cgggcacgac gagcggctcc tcgccccggc ggtcgatgcg gactccggcc
     2701 gctcccagcg tggtcaccca ggcgccgacc ttgtcgagca cgtcgtcctc cgtacagccc
     2761 aggcgctcca ggaccagggc cttctcgtac tcgttggtga acagccactc ggcgccctcg
     2821 accaggcggc gcacctcctc gccgttcagc cgggccagct gctgcgaggg gtcggcggcg
     2881 aagggcacgc cgtggtcccg gcactcctgc gtgaagcgca gcatggccgc gggctcgttc
     2941 gggcagacga gcaccaggtc ggggcgggcg gggccggaca gcatgccgtc caggccggtg
     3001 tccgcggcct cggccatggc gccggcgtag aacgccgcga tctggttgcc gtcctggtcg
     3061 gtgaggcaca tgaaccgggc ggtctgccgt gtcgtcgaca cgtggaccgc gccggtgtcg
     3121 acaccgtgcc gcttgagcca gagctcgtac tcggcgaagt cgtcgccgac cgagccgacg
     3181 agctgggggg tgaggccgag gctgcccagc ccgaaggcga tgttggccgc cactcctccc
     3241 cggcgtactt cgaggtcgtc gacgaggaag gacagcgaga ggtgggcgag ctggtcgggg
     3301 accagctggt ccgcgaaccg gccggggaac accatgagat ggtctgttgc gatggatcca
     3361 gtcaccgcga tgcgcacgtc gtcactcctg ggataggggc tctcaggccg tcaggcacgt
     3421 cactgtcaaa gccctgcggc ggccttcagc tgcgcggccc ggtcggtccg ctcccaggtg
     3481 aacccgtcgt cgtcgcggcc gaagtgcccg tacgcggcgg tggcggcgta gacggggcgc
     3541 agcaggtcga ggtcgcggat gatggcggcc gggcgcaggt cgaagacctc gccgatcgcc
     3601 cgctccaggt ccttctgggg gacggtggcg gtgccgaagg tctccacgaa gaggccgacc
     3661 ggctcggcct tgccgatcgc gtacgcgacc tggacctcgc agcgctcggc gagtccggcg
     3721 gcgacgacgt tcttggcgac ccagcgcatg gcgtaggcgg ccgaacggtc caccttcgac
     3781 gggtccttgc cggagaaggc gccgccgccg tggcgggcgt agccgccgta cgtgtcgacg
     3841 atgatcttcc gtccggtgag gccggcgtcg cccatggggc cgccgatctc gaactttccg
     3901 gtggggttca ccaggaggcg gtagtcgtcg gtcgccagct tgatgccgtc ggccgcgagc
     3961 tgcgccagca cgtgctccac gacgtggagc cggatgtcgg gggcgaggag ggagtccagg
     4021 tcgatgcccg gggcgtgctg ggaggagacg acgatggtgt ccaggcggac gggccggtcg
     4081 tcctcgtacg cgatggtgac ctgggtcttt ccgtcggggc gcaggtaggg gacgatcccc
     4141 tccttgcgga cctccgtgag gcggcgcgac agccggtggg ccaggtcgat cggcagcggc
     4201 atcaaggagg gggtctcggc cgacgcgtag ccgaacatca ggccctggtc gccggcgccc
     4261 tgcttgtcca gctcgtcgcc cgcgtccttc cgggaggcgc cctcgacgcg cttctcgtag
     4321 gcggtgtcca cgccctgcgc gatgtcgggc gactgctggc cgatggagac ggagacgccg
     4381 caggacgccc cgtcgaaccc cttcgcggag gagtcgtatc cgatgtccag gatcttctcg
     4441 cgcacgaggg tggcgatgtc ggcgtacgcc gaggtcgtca cctcgcccgc gacgtgcacc
     4501 tggccggtgg tgaccagggc ctccaccgcg acacgggagt tcgggtcctg ggcgagcagc
     4561 gcgtcgagga tggtgtcgct gatctggtcg gcgatcttgt ccggatgtcc ctcggtgacg
     4621 gactccgagg tgaaaaggcg tcgggacatg gctctcctga gtgacgaggg catgggtgag
     4681 tcgggggacg gtgcggtgag cagggtccgg ctcaagcatg tgcggggagt gcggccggcc
     4741 tccagacgat ccgttgcagg actctgtcgg gatcggcaag cggccggtgg cacttcccgg
     4801 tgccgccaag tcggggtcag atctttgcca agagctccgg gtccggtggc tggtccaccg
     4861 gacccggagc gtcggttcgc aggcggggcg gtacgtcgtg gtgccggggt tcgtccgccg
     4921 gcgccctcac ccgagtcgac ggcgtactcg gcgacggcgt actcggcgac gggtacgcgt
     4981 gctgcggggc ggacgacgga tcagcgggtc cggggcgcca cgacgaggtc ggtggtgaag
     5041 accggcttgc cccgctgggt tccttccacc ctgaccggca cgccgtccgc gcgttcgccc
     5101 ggaacggtcc gggcctcgat ccagcacggc gcctcgaact cggcgtagca ggtgaaggtg
     5161 ctcctcaggc ccagcaccag cgcgtcgggc atgccgctga ccgcgtgcgc ggcctggcgg
     5221 gcggcctcca ggaggaccat gcccgggacg tggtccaccg ggtggtcgaa gtaggtgggg
     5281 tgggcggtgt cgacccgcag ttgccagcgg ccggccgtgc ccggcgcggg cacgccgagc
     5341 acgacgtgcc gggggctggt gtgccccacg agggccggct cgacggccgg tggcatcggt
     5401 tgatcgctgt gggtgggcgt gtcgccgcgc agccgctgga acacctgcgg gctcatgcac
     5461 ctgaaggacg cctcggcggt gccgagtctt ctgccgtccc gctccacggt gacgacgtaa
     5521 cccatggagg acagcgcgcc gttgcggcgg acgatgtccc ggcactcggt gcgcaggacg
     5581 acctcggtgg gcgtgttccc cgcccgcagc agctcgggtt cgagcgagac cgacatgtcc
     5641 cgcatcagga actggtggcc gaaggggacg tcgaactcgg cgtgggccag cagggatccg
     5701 acctgtcgca ccgactcgat caggagcagc gggtcctgga actcgccgtc gcgggcgaac
     5761 agggagtggc tgcgcggcca ttgggcgcgg accacgaagc tctcggaacc gccccgcggg
     5821 tcgacggggc cgatggcgtc ccaccccgtc aggaagacct cggagaccgc cgcgcggtgg
     5881 acgtactccc gcgggaccgt cgacgtcagg cgctgcgccg ggacccgggt taagtcctgc
     5941 tcgaccaccg agacccgtcc cgttcccatg gcccgtgccg tggacgactg ctcagcgaag
     6001 tgcggcatgt gcaggactcc tgtcgtatgc gggcagggga cacctacggc acgccgtcgc
     6061 cccccgctgc ggcaaacctt ccgtgcccaa catacagaca ggtctgcgtg gtcctggtta
     6121 acaacttccc aagctttttg gatcacctgt cccagacgcg gtgccatcta gcacgccgca
     6181 gccacttcag ggcacgtatt cacccaacgg ggcccttgac acgcgggggt ggaagcccct
     6241 tgacattcag acacgtctgt ttgttatctt cctcgtcgcc gactgcagcg ccccagccgc
     6301 tgcggccacg ccacaggagg aacagatgaa tcggcaggcc gtggatctcc tcacttcgcc
     6361 gaccgagcac gcccgccccc gacatccaca gggcggcccc tcccggcccc acttcgccgc
     6421 ctccccgacc cctcaccggc aggaaggact acgcgtcctg gtcgtcgagg ccaacacccg
     6481 ggacgccgac acgctcctcc agggactgac caggcaggga tatgtgacgc agagtgcgaa
     6541 gaacggcgcc caggcgctcc gctcccactc cgacgccgac ctcgtcctgc tcgacctcga
     6601 cctcccggac ctcgacgggc tcgaagtgtg ccggggcatc cgcaccgtca gcgagacgcc
     6661 gatcatcacc gtgacggcgc gcgactccga gctcgaccgc gtcctcggcc tgcaggccgg
     6721 ggcggacgac tacctggtca agccgtacgg gttccgggag ctgctggccc ggatggaggc
     6781 catcatgcgg cgggtccacc gccagaccac gccggcgcgc gtcgccgtcc acctgggcct
     6841 gcgcgtcgac gccgacgcgc ggacggccac gctggacgac gagccgctgg acctgacccg
     6901 caaggagttc gacctgctgt tcctgctcgc ctcgcagccg gacaccgtga tctcgcgccg
     6961 ccagatcatg gcccaggtct ggggcgacgc ctggtccccc cggggccgta ccatcgacac
     7021 ccacgtcggg accctgcggg gcaagctcgg ctccagcgag tggatacaca ccgtccgggg
     7081 catcggcttc cgcctcgggt gcccgtaatc gggccgggag aggccgatca gcgtcgatcc
     7141 gcctccgtcc cgcctccacc ccaccgcagc cccgcgtcac gttgcgttaa gatacagaca
     7201 agtacgtcaa aatttttcgc ggcgagagag tgacatggcc aaacaagaac gggcacagcg
     7261 gacgcgtcaa gccatcctca ccgccgcggc ggagctgttc gacgagcgcg gttacgactc
     7321 ggcgagcacc accgacatcc tggcgcgggc cggcctcacg cgcggggcgc tgtaccacca
     7381 cttcccgtcg aaggaagcga tcgccctcgc cctgctcgac acgcacatcg acgcgctcca
     7441 ggtggaggag cagccggtga agctccagag cctgatcgac ctgaccttca ggttcgccca
     7501 gcagctgcag agcgatccgg cgctccgcgc cagcgtccgc ctctccgtgg agcagtcctc
     7561 gttcgccgcg ttccagaccg gctacgagca gtccatcgag gtcatacgga gcctcctgcg
     7621 gcaggccatg gaccagggcg agatgctgcc cggtctcgac atcgacgagg cgacactctt
     7681 catcgtcggc gcctacaccg gcgtccagac gatggaccag gcgtacagcg gccgcgccga
     7741 gctcatcgac cggatcggct ccctctggcg cttcgtcctg ccgggcctgg ccacccccgg
     7801 cctcatcagc cgcctgcgga ccacaccccc ggcgggcgcc ccgcggcctt cgtcgacctg
     7861 aaggccgctc agccctccgc gggccgcggc accgacggcc gagcccgcac cacgccgcga
     7921 ccgccggaat caccggaacc gccatcggaa tcgaacagcg cctgcagcat cggccacagc
     7981 cggtccacgc tgcgcccctc ccaccacctg gggtcactgc gccccagggt ctccacgccc
     8041 gcggcgagca gcacgacgag atcggcgagg tcctcgaccg ggtacgcggc ccgccgcccg
     8101 gcgagcacga ggcgctcgtg gagcatggcc agcatctggt cccgcagccg gggcgactgg
     8161 aacgtcccgt cgcccgccag cttcatcccc gcccgcacga tcggatccgt acgcagcgac
     8221 tcgcccaagg tgacgaggaa actccgcagg gcgtccgccg gctcccggcc gaacgcctcg
     8281 tcgatgagcg cgacgatccg gtggtgcgcc tcctcgtaga ccgcggcggc caacatcgcg
     8341 gtggaggcga agtggtggta gagagcaccc ctgctcaccc ccgccctctg gcagacgttg
     8401 accagccccg catccgaggc cggcccgtcc gcgaccagct cggccatggc ccgcacaagc
     8461 gcttgccggg tccggtgcga ccgggtctgc atggcccctc atttcatggc atcagtcgaa
     8521 tgttcaaccc cccgggcgtg acagccacgc ggaatcatac aacactgtct cttctgtggc
     8581 agccctctcc cgctcctccc caccaccagg cacagcgaca ggactctttc gccggaatca
     8641 cggccacttg tcgcccggcc tccccccgat ccaccccctg cgccgagtaa acaaaacagg
     8701 gaagtcggtt tacacccctc ccccaccgcc gccaagctcc cctctccgcc cccaaccgac
     8761 tgaactcccc ccgctcgaca gcccatcccc accccctacc ccccctccag caagcctcaa
     8821 gaactcctca agggcgcctc aagcacaact ccagcgcgac acgaccacgc caccctcacg
     8881 aggcggccga gggcggcgaa cacgccttcg gagacggggg cgacccgacc tggacgatga
     8941 gccacctctc cccgccgtac caccaccaag ggcagggact gggacgcggt tgcggcgtcc
     9001 cggttcaggt ccccgtaccg gatgcgcggc ccgttccgga tcatcctgtg gcggctcgca
     9061 ctcgccttcg ggctcatcgc cctcgccgcc gccgtggtct tcctcggccg gagcggctac
     9121 cgggactcgg cggggcaccc ggtcggccgg ctgacgagct tctcctacgc ggcggtcacg
     9181 ctcaccacga tcgggtacgg ggacgtcgtt ccggtcagcg ggacggcgcg gctcgtgaac
     9241 actctggtga tcacaccgct gcggctgggc ttcctgctga tcctggtcgg caccacgatc
     9301 agcgcgctga cggagcggac ccgggccgag tggcgtgagc agtcctggag gcgcaaggtg
     9361 cggggacaca cggtggtggt cggctacggc accaagggcc gggcagcggt ggtcacgctg
     9421 cgggcccgtg gaacccggcc ccgggagatc gtcgtggtcg acgtcagcgc ggcggcggtc
     9481 gacgaggcga accgccacgg tctggtcggc atcgtcggcg acggcacccg taccgacgtg
     9541 ctcgaacggg ccgagctcgg ccgggcgggg cacgtgctga tcgccaccac ccgcgacgac
     9601 accgcggtcc tcgccgcgct gaccgcccgc cggatgaacg gcgccgccgt catcacctcc
     9661 gcggagaccg ccggccatct gctcggcgtc gcctcgctca gcccggcggt cggcaccgtc
     9721 ctggaggaac tcctgtccac cgggctgggc ctggagatcg ccgagcggcc tccggccccg
     9781 gccgaggtcg gccacgcccc cgggcagatc gccgagcccg tcctggccgt ggtccgggac
     9841 ggcagcccgt tgcgctacaa cgacccccgg atcggcgcga tccgccccga cgaccgcctg
     9901 gtcaccgccc gcgaaccgcg ccggggcccg tcaccggact gagccacggg ctgttccccg
     9961 ggcaggggca gggggcaggg ggcagggggc agggagtcgg ccgtggaggg ggcagccgtg
    10021 gaggggggtc agccgtggag gggggcgtcc gtggcgtgtt cccgtaccgc cctttccgcc
    10081 tcctcctcgg acaccccgac gacgacgagc aggtcgcgta ccgagagtcc cagggcgagt
    10141 cgcgccgcct cggcccgcgt ccccgcctcc tgccgagcgc ttgccccgtt gctcgtatcc
    10201 gcaccgttcc gcgtatccgc ctccgcgggg aagcgcgcgg ccacggcgag cgcggaccgg
    10261 acgcaacggt ggcgcgcggg ggcgtcgggc gccgccgccg cgtacgtctc cagcacatcg
    10321 gccagctctt ggatgccggc gtcgagcggt ccctcggtgc cgggtccggg ctcggcggcg
    10381 agacgggcca ggagcaggca actgttgccc agcatgccga gccgcagcgc ggccttgctc
    10441 atggcctgga ccgagccccg gtgcaggcgc cagcgcgggg tgcgccgggc gacgttcacc
    10501 atgtcggtac gggcctggtc cacatcgcgc aggagctggt acagcgtgcg tgattccagc
    10561 ggcagcacct cctcgggatc gccgccgtgg ccccggacac cgagccgcag gagcctggcc
    10621 agtccccgca gggcggcgtt ctccgcccgg cgcagcagct tcagcgggtg gacggggaag
    10681 aggagctggc tgaagatcag ggcgaccgcg gcccccacca gcacgtcctc gatccgttcg
    10741 atcccggcca gttgcccgga cgcgacggcg atcaccgcgc tcaccccggc ctgcgccatg
    10801 gtcacccgtt cgccgtcgag caccagggcg gccagcaggg cgaccagcac cgcgcagccc
    10861 acggccacgg cccccgccgt tcggccgagc agtacgtacg cggcctgacc gatcagcacg
    10921 ccgcacacca ctccgaacac cacacgcacg gcgttggtgc cgcggccgcc cagctgggtg
    10981 ttgagcgcca ccagcgtggc gatcggcg
//
