LOCUS       NZ_JABBGE010000010      5832 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig10, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000010
VERSION     NZ_JABBGE010000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 269498)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 269498)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 263666
            Orig. end                         :: 269498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..5832
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..5832
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5832
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5411](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5411)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            204..1007
                     /locus_tag="HHL19_RS29675"
                     /old_locus_tag="HHL19_29685"
     CDS             204..1007
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHL19_RS29675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HHL19_29685"
                     /product="hypothetical protein"
                     /protein_id="WP_169093443.1"
                     /transl_table=11
                     /translation="MATNTTENTGFPETNRRRIARGHRGWGLLCVLLGLLALGGAVGLG
                     AMLPERIDAERAYLDAVPCAAGERATAADADCLRTVRGTVESVEKVTSGKARFLRVSLR
                     PPVAAPLDRPLELSTRGELSEVLGPGDEVTVTLWRDLRVSAHHAGRSEDVDARPDSEVG
                     PLVGLAAACAWLAAVAFTGAYGGERRARGIASGRPVQAKVGFGFAKLGGILGVPFLLGI
                     VVGKIWDGWTTVLVTAGVTALIAVQATVFALRVDRPQTRRRGRVT"
     gene            complement(1000..1794)
                     /gene="ehuA"
                     /locus_tag="HHL19_RS29680"
                     /old_locus_tag="HHL19_29690"
     CDS             complement(1000..1794)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563991.1"
                     /locus_tag="HHL19_RS29680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29690"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_169093444.1"
                     /transl_table=11
                     /translation="MPREYAPAGAEKVRFDRVTKRFGDVTVLDGLDLTVAPGERVTLIG
                     PSGSGKTTILRLLMTLERVTEGVVHVDGEPFSHMPSGPGGALVPADERYLAPRRRRIGM
                     VFQQFNLFPHMSVLANVMEAPRHVLGLDGDEAAARARDLLGLVGLADKEDAHPTRLSGG
                     QQQRVAIARALAMRPELLLLDEVTSALDPELVAEVLDVLRDVADSTDITMLCVTHEMGF
                     ARDISDRVLMFDGGHVVESGPPEGLFDAPEHERTRAFLRSIM"
     gene            complement(1784..2428)
                     /gene="ehuD"
                     /locus_tag="HHL19_RS29685"
                     /old_locus_tag="HHL19_29695"
     CDS             complement(1784..2428)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968950.1"
                     /locus_tag="HHL19_RS29685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29695"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_169093445.1"
                     /transl_table=11
                     /translation="MGGMWDMEAARAALPVVLSGFGVTLLATVLGFAVAVVAGLAIALL
                     QRSGGRWLALAVGAPAAFVRSTPVMVQLFAAWVLVPGLDPLTLGVLVLGLHYATYLSEV
                     YRAGIDAVPKGQWEACTALSLPRRRVWRAVVLPQAVRKVLPALGNYAVSMFKETPFLSV
                     ITVNEMVHEASTFGSTHFAYLESFTLAAAVFLLASWPTSLLVRRLEARLAP"
     gene            complement(2428..3105)
                     /gene="ehuC"
                     /locus_tag="HHL19_RS29690"
                     /old_locus_tag="HHL19_29700"
     CDS             complement(2428..3105)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124793.1"
                     /locus_tag="HHL19_RS29690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29700"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_169093472.1"
                     /transl_table=11
                     /translation="MAVFLSELGRALPAIGQGLLVTLEATALGAALALLVAFALGLASR
                     SRFLVVRGATRVTVEFLRGTSLYVQLFWLFFALPMLGFRLEPMACGVLAFGLNYGAYGA
                     EVVRGAIAAVPAAQTEAAIALGMGPGLRLRRIVLPQAYPLMVPPFKNLLIQLLKATPLL
                     SLVTVADLTFTIDQLRSATGGTAAAYLLLLLLYFALAALLGAGMDALERRAKARLGVTG
                     GGA"
     gene            complement(3117..4007)
                     /gene="ehuB"
                     /locus_tag="HHL19_RS29695"
                     /old_locus_tag="HHL19_29705"
     CDS             complement(3117..4007)
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563988.1"
                     /locus_tag="HHL19_RS29695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29705"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_169093446.1"
                     /transl_table=11
                     /translation="MTTHLSRRHLLGALAVPGLLAACSRTEAGTGAPGGDADLLEKLRK
                     QGHVRVGFAGEAPYGFQDGGELAGEAPTLHREIFTALGIPELKPTLTEFGALIPGLLAG
                     RFDVVSAGMAITPERCAKVAFSEPEFVSPTALMVRKGNPKGLSDLASCAAKNARVGVLT
                     AAVESGYAKAAGVGDGALVSLAKQQDGLDALLAGRIDAFALTGISLRWLARTNEGKAVE
                     VLEPFVPVVDGVRQYSPGGAVFRQGAVSLREAFDRELARITGDPARYVKLIGKYGFTER
                     EVPPSTLRTAELCAG"
     gene            complement(4049..4948)
                     /gene="thpD"
                     /locus_tag="HHL19_RS29700"
                     /old_locus_tag="HHL19_29710"
     CDS             complement(4049..4948)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="HHL19_RS29700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29710"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_169093447.1"
                     /sec_met_domain="PhyH (E-value: 6.2e-38, bitscore: 125.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPARPVDPYPTRGTEEALVYRKEPVVWSEEGTPGPFWPRELE
                     DYERNGFFAVDALVTPEEVTELRAELDRLVTDPELRNDERAIVEPRSQEIRSVFEVHRI
                     SEVFARLATDPRLVDRARQILGSDVYIHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     LMRTVSVSIALTPNFTTNGSLMIMPGSHRTFLGCAGETPKDNYKRSLQMQDAGTPSRKA
                     LTTFAEACGIEHFTGDAGSAVWFDCNAMHGSGDNITPFPRSNVFLVFNSVENAPELPFA
                     APVRRPSYIAARDTTPIG"
     gene            complement(5001..5411)
                     /locus_tag="HHL19_RS29705"
                     /old_locus_tag="HHL19_29715"
     CDS             complement(5001..5411)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031435.1"
                     /locus_tag="HHL19_RS29705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29715"
                     /product="ectoine synthase"
                     /protein_id="WP_169093448.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.3e-54, bitscore:
                     175.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTFAELENTERHVRSASGTWESKRIVLARERVGFSLHETVLY
                     AGTETAMWYANHVEAVVCTAGEAELTDHETGTTYTITPGTMYLLDGHERHSLRVKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEAGDA"
     CDS             complement(5438..>5828)
                     /GO_function="GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="HHL19_RS29710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_29720"
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_169093449.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.4e-18, bitscore:
                     59.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELDVWQPGEHNGTFRGNNPAFVTATAALDTYWRDSTLRDRTLRRA
                     ATVEAALGELGPVAVPRGRGLVWGLEFEDGERARAVCRRAFELGLLIETSGPRDEVVKL
                     LPPLTVSDDELDEGLGILARAVRHTA"
     gene            complement(5438..>5832)
                     /locus_tag="HHL19_RS29710"
                     /old_locus_tag="HHL19_29720"
ORIGIN
        1 atccccggcc acaacgcggc gatggcggcg ctgggccgct gacgggccgt gccgaacggc
       61 gccacgttgc cggaggccgg tacccacggg ggcgccaggc tgctggagcc gtacatgcca
      121 ggtgatcgcc gttccgtggc tcaatatggt cggtccggga ccgcgggagg cgggcccggg
      181 cgtcgctagg gagggcgaca cggatggcga cgaacaccac ggagaacacg ggattcccgg
      241 agacgaaccg gcgcaggatc gcccggggcc atcgtgggtg gggactgctc tgcgtactgc
      301 tcgggctact ggccctgggc ggcgcggtgg gcctcggcgc gatgcttccc gaacggatcg
      361 acgccgaacg ggcctacctc gacgccgtgc cctgcgcggc cggggagcgg gcgacggccg
      421 ccgacgcgga ctgtctgcgc acggtacggg gcacggtgga gtccgtcgag aaggtgacct
      481 ccgggaaggc ccggttcctg cgggtgagcc tgcgtccccc ggtggcggcc ccgctggacc
      541 ggccgctgga gctgagcacg aggggcgagc tgtcggaggt cctcgggccc ggggacgagg
      601 tgacggtcac cctctggcgg gacctgcgcg tctccgccca ccatgccggc cgcagcgagg
      661 acgtcgacgc gcggccggac agcgaggtgg ggcccctcgt cgggctcgcc gccgcctgcg
      721 cctggctggc cgcggtcgcc ttcaccggcg cgtacggcgg tgagcggcgc gcccgcggta
      781 tcgcgagcgg ccggcccgtg caggcgaagg tcggcttcgg cttcgcgaag ctcggcggca
      841 tcctgggcgt cccgttcctc ctgggcatcg tcgtcgggaa gatctgggac ggctggacga
      901 cggtcctcgt cacggcgggc gtcacggcgc tgatcgccgt ccaggccacg gtcttcgccc
      961 tccgggtcga ccgcccgcag acccggaggc ggggccgcgt cacatgatcg accgcaggaa
     1021 ggcccgcgtc cgctcgtgct ccggcgcgtc gaacaggccc tccggcgggc ccgactcgac
     1081 gacgtgaccg ccgtcgaaca tcaggacgcg gtccgagatg tcgcgggcga agcccatctc
     1141 gtgcgtgacg cagagcatgg tgatgtcggt gctgtcggcg acgtcccgca ggacgtccag
     1201 gacctccgcc accagctcgg ggtcgagggc cgaggtcacc tcgtccagca acagcagctc
     1261 cgggcgcatc gcgagcgcgc gggcgatggc gacgcgctgc tgctggccgc cggagagcct
     1321 ggtcgggtgg gcgtcctcct tgtcggcgag gccgacgagg ccgaggaggt cccgggcgcg
     1381 ggcggcggcc tcgtcgccgt cgagacccag gacgtgccgg ggcgcctcca tgacgttcgc
     1441 caggacgctc atgtgcggga agaggttgaa ctgctggaac accatgccga tgcggcggcg
     1501 gcgcggggcg aggtagcgct cgtccgccgg gacgagcgcg ccaccggggc cggacggcat
     1561 gtgcgagaac ggttcgccgt cgacgtggac gacgccttcc gtgacccgtt ccagcgtcat
     1621 cagcagccgc aggatggtcg tcttccccga gccgctcggg ccgatcaggg tcacccgctc
     1681 gccgggcgcg accgtcaggt ccagtccgtc gaggaccgtg acgtccccga atcgcttcgt
     1741 cacccggtcg aaccggacct tctccgctcc ggcgggagcg tactcacggg gcaaggcggg
     1801 cctccagtcg gcgcacgagc agggacgtcg gccagctcgc cagcaggaag acggcggcgg
     1861 ccagggtgaa gctctccaga taggcgaagt gggtgctgcc gaaggtgctc gcctcgtgca
     1921 ccatctcgtt caccgtgatg accgacagga acggcgtctc cttgaacatg gagaccgcgt
     1981 agttgccgag cgccggaagc accttccgta cggcctgagg cagcaccacc gcccgccaca
     2041 cccgccggcg cggcagcgac agcgccgtac aggcctccca ctgccccttc ggtacggcgt
     2101 cgatgccggc gcggtacacc tcggacaggt acgtggcgta atgcaggccc aggaccagga
     2161 cgcccagcgt gagcgggtcc aggccaggca ccagcaccca ggccgcgaag agctgcacca
     2221 tcaccggcgt cgagcgcacg aacgcggccg gcgcgcccac ggcgagggcg agccaccggc
     2281 cgcccgagcg ctggagcagc gcgatggcga gcccggcgac gaccgcgacg gcgaagccga
     2341 gcacggtcgc cagcagggtg accccgaaac cggacaggac gacgggcagc gctgcccgcg
     2401 ccgcctccat gtcccacatg cctcccatca ggcaccaccg cccgtgacgc cgaggcgcgc
     2461 tttcgcacgc cgctccaggg cgtccatccc ggcccccagg agcgcggcga gcgcgaagta
     2521 cagcaggagc agcagcagat acgcggcggc cgtgccgccc gtcgccgacc gcagctggtc
     2581 gatcgtgaag gtcaggtcgg cgaccgtcac cagggacagc agcggggtcg ccttcaggag
     2641 ctggatcagg aggttcttga agggcggcac catcagcggg tacgcctgcg gcagcacgat
     2701 ccgccgcagc cgcaggcccg gccccatgcc cagggcgatc gccgcctcgg tctgcgcggc
     2761 gggcacggcc gcgatcgcgc cgcgcaccac ctccgccccg tacgccccgt agttgaggcc
     2821 gaaggcgagc accccgcagg ccatcggctc cagccggaag ccgagcatcg gcagcgcgaa
     2881 gaagagccag aacagctgga cgtacagcga agtgccgcgc aggaactcga cggtgacgcg
     2941 ggtggcgccg cgcacgacga ggaagcggga gcgggaggcg aggcccaggg cgaaggcgac
     3001 gagcagcgcg agggccgcgc cgagcgccgt ggcctccagg gtgaccagca gtccctgtcc
     3061 gatcgccggg agggcccggc ccagctcgga caggaacacg gccatgccgg attcggtcat
     3121 cccgcgcaca gctccgcggt gcgcagggtg ctcggcggta cttcgcgctc ggtgaagccg
     3181 tacttcccga tcagcttcac gtagcgggcc gggtcgccgg tgatccgggc cagctcgcgg
     3241 tcgaacgcct cccgcagcga gaccgcgccc tgccggaaga ccgccccgcc ggggctgtac
     3301 tgccgcaccc cgtcgacgac cggtacgaac ggctccagga cctccacggc cttgccctcg
     3361 ttggtgcggg cgagccagcg cagcgagatg ccggtgaggg cgaaggcgtc gatccggcct
     3421 gcgagcaggg cgtcgaggcc gtcctgctgc ttggcgagcg acaccagggc cccgtccccg
     3481 acgcccgccg ccttcgcgta cccgctctcc acggcggccg tcaggacgcc gacacgggcg
     3541 ttcttcgccg cgcaggaggc gaggtcgctc aggcccttcg ggttgccctt gcggaccatg
     3601 agcgcggtgg gggagacgaa ctccggctcg gagaaggcga ctttggcgca gcgttcgggg
     3661 gtgatggcca tgccggcgct gacgacgtcg aatcgtccgg cgagcagtcc ggggatgagg
     3721 gcgccgaact cggtgagggt gggcttgagt tcgggtatgc cgagggcggt gaagatctcc
     3781 cggtggaggg tgggtgcctc gccggcgagt tcgccgccgt cctggaagcc gtacggtgcc
     3841 tcgccggcga agccgacccg gacgtggccc tgcttccgca gcttctccag caggtccgcg
     3901 tccccgccgg gcgcgcccgt gccggcctcc gtgcgcgagc aggcggccag caggccgggc
     3961 acggcgaggg cgccgagcag gtgtctgcgg gagaggtgcg tggtcatgtg cgtcctttcg
     4021 cagggggggg gcggggcagg aaggagggtc agccgatcgg cgtggtgtcc cgggcggcga
     4081 tgtaggaggg tcggcggacg ggggcggcga agggcagttc gggtgcgttt tcgacgctgt
     4141 tgaagacgag gaagacgttg ctgcggggga agggggtgat gttgtcgccg gagccgtgca
     4201 tggcgttgca gtcgaaccag acggcggatc cggcgtcgcc ggtgaagtgt tcgattccgc
     4261 aggcttcggc gaaggtggtg agtgccttgc gtgagggggt gccggcgtcc tgcatctgca
     4321 gggatcgctt gtagttgtcc ttgggggtct cgccggcgca gccgaggaag gtgcggtggg
     4381 agccgggcat gatcatgagg ctgccgttgg tggtgaagtt cggggtcagt gcgatcgaca
     4441 ccgagacggt gcgcatcagg gggagtccgt cttcggcgtg ccaggtctcg aagtcggagt
     4501 gccagtagaa gccgctggcg ccgaagccgg gcttgacgtt gatccgtgat tggtggatgt
     4561 agacgtcgga gccgaggatc tgtcgggcgc ggtcgacgag tcgggggtcg gtggcgaggc
     4621 gggcgaagac ctcgctgatc cggtggactt cgaagacgga gcggatttcc tgggagcggg
     4681 gttcgacgat ggcccgttcg tcgttgcgga gttcggggtc ggtgaccagg cggtcgagtt
     4741 ccgcgcggag ttcggtgacc tcttcggggg tgacgagggc gtcgacggcg aagaagccgt
     4801 tgcgctcgta gtcctcgagt tcgcgcggcc agaacgggcc gggcgtgccc tcctcggacc
     4861 agacgacggg ttccttgcgg tagacgaggg cttcctcggt gccgcgggtg gggtacgggt
     4921 cgacggggcg ggcgggggcg gtggtcatcg cggtgcgggc ctttctcgta cgtacggggg
     4981 aggggcggtc gatcgggggg tcaggcgtcg cccgcctcgg gctcggtcag cagcgggtag
     5041 acgccgttct cgtcgtggtc ctcgcggccg gtcaccggcg ggttgaagac gcacaggcag
     5101 cggaagtcct ccttgacccg gaggctgtgc cgctcgtggc cgtcgaggag gtacatcgtg
     5161 ccgggggtga tcgtgtacgt cgtcccggtc tcgtggtcgg tcagttcggc ctcgccggcg
     5221 gtgcagacga ccgcctcgac gtggttcgcg taccacatgg cggtctcggt cccggcgtac
     5281 aggacggtct cgtggaggga gaagccgacg cgctcgcggg cgaggacgat gcgcttgctc
     5341 tcccaggtgc cggaggcgga cctcacgtgc cgctcggtgt tctccagctc ggcgaaggtg
     5401 cggacgatca tggggagttg tccttctctc gggggtctca ggcggtgtgg cggacggcgc
     5461 gggcgaggat gccgaggccc tcgtcgagct cgtcgtcgct gacggtcagc ggcggcagca
     5521 gcttgacgac ctcgtcgcgc ggacccgacg tctcgatgag caggcccagt tcgaaggcgc
     5581 gccggcacac cgcgcgggcc cgctccccgt cctcgaactc caggccccac acgagccccc
     5641 ggccgcgcgg cacggccacc ggcccgagct cgccgagcgc cgcctcgacg gtggcggccc
     5701 gccgcagggt gcggtcgcgg agggtggagt cgcgccagta ggtgtcgagg gcggcggtgg
     5761 cggtgacgaa ggcggggttg ttgccgcgga aggtgccgtt gtgttcgccg ggctgccata
     5821 cgtcgagttc gt
//
