LOCUS       NZ_JABBGE010000004     10954 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig4, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000004
VERSION     NZ_JABBGE010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 676282)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 676282)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 307253
            Orig. end                         :: 318207
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10954
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10954
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10954
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5954](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5954
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(443..458)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.22"
                     /tool="antismash"
     misc_feature    complement(543..557)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.0"
                     /tool="antismash"
     gene            643..1026
                     /locus_tag="HHL19_RS14335"
                     /old_locus_tag="HHL19_14340"
     CDS             643..1026
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037145.1"
                     /locus_tag="HHL19_RS14335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14340"
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="WP_169089419.1"
                     /transl_table=11
                     /translation="MFHTGLRNPGAVRTEESAEGTEIYFQRCAWCQTTTFQRLLCPTCG
                     STDLSPQLSEGEGVITVRRAYTAAEADLWPVHMAEGFVVRCRVDGPPHAVRPGVRVKLA
                     SAAATGRRPVVRLCEEVPLDGWI"
     gene            complement(1092..2048)
                     /locus_tag="HHL19_RS14340"
                     /old_locus_tag="HHL19_14345"
     CDS             complement(1092..2048)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238294.1"
                     /locus_tag="HHL19_RS14340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14345"
                     /product="electron transfer flavoprotein subunit alpha/FixB
                     family protein"
                     /protein_id="WP_169089421.1"
                     /transl_table=11
                     /translation="MAEILVLVDHADGAVRKPALELLTLARRLGEPSAVVLGAGEATAS
                     VAATAAEYGAATVYVADGAEFAEQLVVPKVDALTQIAKEKEVAAVLVTSSGEGKEIAAR
                     VALRLGSGLITDAVDLEAGAEGPVATQSVFAASYTTKSRVSHGVPVITVKPNSTAPEAA
                     PAAGKVEDVTVAFTGNAAKVVSRTARVSTGRPELTESAIVVSGGRGVGAAEGFEVVEKL
                     ADSLGAAVGASRAAVDAGWYPHSNQVGQTGKQVSPQLYVAAGISGAIQHRAGMQTSKTI
                     VAVNKDPEAPIFDLVDYGVVGDLFEVLPQLTEEIGSR"
     gene            complement(2082..2870)
                     /locus_tag="HHL19_RS14345"
                     /old_locus_tag="HHL19_14350"
     CDS             complement(2082..2870)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262570.1"
                     /locus_tag="HHL19_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14350"
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="WP_169089422.1"
                     /transl_table=11
                     /translation="MTLRIAVCVKYVPDATGDRGFADDLTVDRDEVDGLLSELDEYAVE
                     QALRISEEADEAEVTVVTVGPDDAKDALRKALSMGADKAVHVNDEAIHGSDVIATSAIL
                     AKALEKTGFDLVVCGMASTDGTMGVLPALLAERLSLPAATLLSEVSVEDGVVKGRRDGD
                     AATELLEAPLPAVVSVTDQSGEARYPSFKGIMAAKKKPVEELELDDLDIDEDEVGLAGS
                     WTLVESADARPARTQGTIVTNEDGDGGKQLAAFLASQKFI"
     gene            complement(2899..4050)
                     /locus_tag="HHL19_RS14350"
                     /old_locus_tag="HHL19_14355"
     CDS             complement(2899..4050)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037148.1"
                     /locus_tag="HHL19_RS14350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14355"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_169089424.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 6.7e-32, bitscore:
                     105.7, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2.6e-29, bitscore:
                     96.5, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.4e-52, bitscore:
                     171.8, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKDFDLYRTSEEHEMLRESVRALAEAKIAPFAAAVDEEGRFPQE
                     ALDALVANDLHAVHVPESYGGAGADALATVIVIEEVARVCGSSSLIPAVNKLGSLPVVL
                     SGSEELKAKYLAPLAAGEAMFSYALSEPDAGSDAAGMKTRAVRDGDFWVLNGVKRWITN
                     AGVSEYYTVMAVTDPSMRSKGISAFVVEKSDEGVSFGAPEKKLGIKGSPTREVYFDNVR
                     IPADRMIGAEGTGFATAMKTLDHTRITIAAQALGIAQGALDYAKGYVKERKQFGKPIAD
                     FQGVQFMLADMAMKLEAARQLTYAAAAKSERVDGDLTFFGAAAKCFASDVAMEVTTDAV
                     QLLGGYGYTRDYPVERMMRDAKITQIYEGTNQVQRIVMARNLP"
     gene            complement(4195..4872)
                     /locus_tag="HHL19_RS14355"
                     /old_locus_tag="HHL19_14360"
     CDS             complement(4195..4872)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037149.1"
                     /locus_tag="HHL19_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14360"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_169089426.1"
                     /transl_table=11
                     /translation="MAQQARAIRTRRTILEAAATVFAERGYERATVGEILARAGVTKGA
                     LYFHFASKEEVAMGVLDAQLLDEPLTPQEVKLQELIDQGFLLAHRLQHDPLVQASVALA
                     LDSGAVSVNRAIPFQAWVDQVSDILTVARARGELLRHVNVTDTAELFSGAFTGIQTMSA
                     VLCDRNDLPHRTTVLMKHLIPSICTPSILLELDMSAERGRALAAEYEAGRQHRDDVSSD
                     VHA"
     gene            5001..5954
                     /locus_tag="HHL19_RS14360"
                     /old_locus_tag="HHL19_14365"
     CDS             5001..5954
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037150.1"
                     /locus_tag="HHL19_RS14360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14365"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_169089428.1"
                     /sec_met_domain="AfsA (E-value: 2e-24, bitscore: 80.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRHEPQGETKGTSVPGEFVHRHDPLDIIPTGWTRLAENRFSVSA
                     AWSTGHPFFSPVDGDRHDPILVAETIRQATMLIAHAEYDVPVESQFVLWDLSYDAEPDA
                     LLVDDDGADLTVDVILSDVRGRGRGLRALRTTTVLTRDGRLVATGRGVASCTSAAAYRR
                     IRGPQLALLGRPVPLITGVAPHSVGRERPEDVVLGAGALPGQWQLRIDTGHPTLFRRPN
                     DHVPGMLLLEAARQAAHATFGSEKFFPSVMNVSFHRYAELDTPCWITAEVVPALDPETY
                     AVRISGRQDGQSVFTCTFTSPTGALAPVEVDHRLAS"
     gene            5994..6971
                     /locus_tag="HHL19_RS14365"
                     /old_locus_tag="HHL19_14370"
     CDS             5994..6971
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (6-171). E-value: 7.9e-13.
                     Score: 40.0. Matches aSDomain:
                     nrpspksdomains_HHL19_RS14365_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037151.1"
                     /locus_tag="HHL19_RS14365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14370"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_169089430.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.7e-12, bitscore:
                     40.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.4e-08,
                     bitscore: 28.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTRLLVTGATGFIGGRVAAAAAAHPDLDRVRLLVRNPDAAPAAH
                     QDAPAGGPVVETVQGDVRDPASLRRACEGVDAVIHCASAIGGEEELLRAVNEHGTRDLV
                     DAARRAGAGRIVALSTASVHGRGPFRAAAPGTLPLAPVSATSRTRAAGERYVLDAGGTV
                     LRPHLVYGTGDRQVVPGILTLLAALPGPLAGSGSLHSLIEVESLAGALLGAALSPATGP
                     GAYYLAHPDPVSDDELLAAVEPLLPESARAARGPAVEFAEARKLLVGNPLAAHHLEMFG
                     LDHWFADERPWTDFGRGPGPGFTAVFPRHLSWYRRLLADRAERA"
     aSDomain        6012..6506
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HHL19_RS14365_NAD_binding_4.1"
                     /evalue="7.90E-13"
                     /label="HHL19_RS14365_NAD_binding_4.1"
                     /locus_tag="HHL19_RS14365"
                     /protein_end="171"
                     /protein_start="6"
                     /score="40.0"
                     /tool="antismash"
                     /translation="VTGATGFIGGRVAAAAAAHPDLDRVRLLVRNPDAAPAAHQDAPAG
                     GPVVETVQGDVRDPASLRRACEGVDAVIHCASAIGGEEELLRAVNEHGTRDLVDAARRA
                     GAGRIVALSTASVHGRGPFRAAAPGTLPLAPVSATSRTRAAGERYVLDAGGTVLRPHLV
                     YG"
     gene            7017..7622
                     /locus_tag="HHL19_RS14370"
                     /old_locus_tag="HHL19_14375"
     CDS             7017..7622
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037201.1"
                     /locus_tag="HHL19_RS14370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14375"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_169089432.1"
                     /transl_table=11
                     /translation="MTSFRESVRTLLREQVLDAAYGLVAADGWGVLRMTAIARAAGISR
                     QTLYNEFGSKEAIGGALVRRELERFLVGIQRELDAHRGRLERAARAGVEYTLQQAVDNP
                     LIKSVLLAARGGADADLLAYLTTRTEPVFGTAMTLLDAYAIEAWPDVDEESRGLAVETI
                     VRLTVSHIVQPVAPPEESARRIARVTARVAYPGGVPED"
     gene            complement(7632..8849)
                     /locus_tag="HHL19_RS14375"
                     /old_locus_tag="HHL19_14380"
     CDS             complement(7632..8849)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006125586.1"
                     /locus_tag="HHL19_RS14375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14380"
                     /product="sensor histidine kinase"
                     /protein_id="WP_169089434.1"
                     /transl_table=11
                     /translation="MARWDGTGTATGPGGDARSADDEARWFRVLLHTGFFLLLGSSLTR
                     FLMRHTGEGIAPWVVALAAGLAVLYVAEPALAADRSRRHLWLGACTATWIALVFLAPSF
                     AWCSIPLFYLGLRNLRPPVAACFIGLLTVVSVTAQLRLAGRFEFDILLGPPAVAAICVA
                     VFVRMDEQTRRQRELITDLVRTRRELAATERREGTLAERQRLSMEIHDTLAQGLSSQQM
                     LLQAADRTWRSDPDTAHRHVRAATAIAEHNLTEARRFVHDLAPADLAGGGGLDEALRTL
                     ARRESTDGLAVRCHVEGGQGRRLPDRVQSALLRIAQGALANVREHAEARTAALTLTYLD
                     DQVVLDIADDGRGFDPSAARDGDGVRGHGLPAIRTRARQLGGTLTVESAPGEGTVLSAA
                     VPLTGD"
     gene            complement(9076..10167)
                     /locus_tag="HHL19_RS14380"
                     /old_locus_tag="HHL19_14385"
     CDS             complement(9076..10167)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742874.1"
                     /locus_tag="HHL19_RS14380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_14385"
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="WP_346768340.1"
                     /transl_table=11
                     /translation="MSVIQRLRTAVRRTYRRTVDLSHPARSPLGSAVVNCVVYADGVRR
                     TVRGGGADEALRHVRRTGDGFVWIGLHEPAEKELTVLAELFGLHPLAVEDAVHAHQRPK
                     IEEYDGVLFAVLKTVRYVEHEELTATSEVVDTGELMAFVGPDFVITVRHGGHGSLGPLR
                     EALEELPEQLVKGPSAVLHAVVDHVVDDYLAVTDAVQTDLDAIENAVFSAQADRRDAGR
                     IYQLKRELLELRRAVAPLARPLTALATRPMPLVDPEIRAYFRDVTDHLSRVTEQIASYD
                     TLLDSVLQAHLAQVTVAQNEDMRKITAWAAIVAVPTMVCGVYGMNFDHMPELGWTYGYP
                     VVLGVIAVACYVIHRGFRRNGWL"
ORIGIN
        1 cgacatgacc cgcttcctcg ccgccagcgc cgacagcgac gacccgatgg tccgggtctg
       61 cggcgccgcc cgcctggtca tgcggatgta cgccgagaac cccaccttct ccgtccagcg
      121 ctaccggctc acccgggagg tcgccgggct gcgcgcgtac gagctctccg tcgtctggcg
      181 gtacgagaag accctcggcg actacctgcg cgcccgctgg gccgaccgca gggacggcac
      241 cctgcgcgcc aacgtggccg ccgcggccgt cgtcgccgcc cacaaccacg ccctgcggca
      301 ctggctccgc tcgggcggcg agggcgagcc cctcgaagag gtcgaccggg ccctggagtt
      361 cgtccgcgcc acctggagcc cggacgcgcc cggcgccgcc ggcgactccg cggccgaggg
      421 ggacgaggac gtggtggtcg tcatcaccaa gcggtccacc ccgatgtggc gcgtggtgcg
      481 cgaggtcgag tcgagcctcg gcgagggctg aggggtacac acgaacgcct aggggccgca
      541 gtatctcaga ttgagggaac tgaggtcttt ctttgctggc actgagtgcc atatcttggg
      601 ctccatgcac ggtcgagccg ccgagtgcga ggagaaggca tcgtgttcca cacaggactg
      661 aggaaccccg gcgccgtccg gaccgaggaa tcggccgaag gcaccgaaat ctacttccag
      721 cgctgcgcct ggtgccagac caccaccttc cagcgcctgc tctgcccgac ctgcgggtcg
      781 accgacctgt ccccccaact cagcgagggg gagggcgtga tcaccgtccg cagggcctac
      841 accgccgccg aggccgacct ctggccggtg cacatggccg agggtttcgt ggtccgctgc
      901 cgggtcgacg ggccgccgca cgccgtgcgc cccggtgtcc gggtcaagct cgcgagcgcc
      961 gccgccaccg gccgccggcc ggtcgtccgg ctctgcgagg aggtcccgct ggacggctgg
     1021 atctgaaccc gggagggcat gcgaaaaggg ggtggcacag agtgccaccc ccttcgtcgt
     1081 gtccgacggc gtcagcgcga gccgatctcc tcggtcagct gcggcagcac ctcgaagagg
     1141 tcgccgacca cgccgtagtc gaccaggtcg aagatcgggg cctcggggtc cttgttgacc
     1201 gcgacgatcg tcttcgacgt ctgcatgccg gcccggtgct ggatggcgcc ggagatgccc
     1261 gcggcgacgt acagctgcgg cgagacctgc ttgccggtct ggccgacctg gttggagtgc
     1321 gggtaccagc cggcgtcgac ggcggcgcgg gaggcgccga cggccgcgcc gagcgagtcg
     1381 gccagcttct cgacgacctc gaagccctcg gcggcgccga cgccgcggcc gccggagacc
     1441 acgatggcgg actcggtcag ctccgggcgg ccggtggaga cgcgcgcggt gcgggagacg
     1501 accttcgccg cgttgccggt gaaggcgacg gtgacgtcct cgaccttgcc ggcggccggg
     1561 gcggcctccg gggcggtgga gttcggcttg acggtgatga ccggaacgcc gtgcgagaca
     1621 cgggacttgg tcgtgtacga ggcggcgaag accgactgcg tggcgaccgg gccctcggca
     1681 ccggcctcca ggtcgaccgc gtcggtgatg aggccggagc cgaggcggag cgcgacgcgg
     1741 gccgcgatct ccttgccctc gccggaggag gtcaccagga cggcggccac ctccttctcc
     1801 ttcgcgatct gggtgagcgc gtcgaccttg gggacgacga gctgctcggc gaactcggcg
     1861 ccgtccgcca cgtagacggt ggccgcgccg tactcggcgg cggtggcggc gacggaggcg
     1921 gtggcctcgc cggcgccgag gacgaccgcg gacggctcgc ccaggcggcg ggccagggtg
     1981 agcagttcga gggccggctt gcggaccgca ccgtcggcgt ggtccacgag aaccaggatc
     2041 tcagccatga ttccttgctc ctgaggtggg gtgacggacg gtcagatgaa cttctgggag
     2101 gcgaggaagg cggccagctg cttgccgccg tcaccgtcct cgttcgtgac gatcgtgccc
     2161 tgggtgcggg ccgggcgggc gtccgccgac tcgaccaggg tccaggagcc ggccaggccg
     2221 acctcgtcct cgtcgatgtc caggtcgtcg agctccagct cctcgaccgg cttcttcttc
     2281 gcggccatga tccccttgaa ggaggggtaa cgggcctcgc cggactggtc ggtgaccgag
     2341 acgacggccg gcagcggggc ctccaggagc tcggtggcgg cgtcgccgtc gcgacggccc
     2401 ttgaccacgc cgtcctccac cgacacctcg gagagcagcg tcgccgccgg caggctcagc
     2461 cgctcggcga gcagtgcggg gagcacgccc atggtgccgt cggtcgaggc catgccgcag
     2521 acgaccaggt cgaaaccggt cttctccagc gccttggcga ggatcgcgga ggtggcgatg
     2581 acgtcggagc cgtggatggc ctcgtcgttg acgtggacgg ccttgtcggc gcccatcgac
     2641 agcgccttgc gcagggcgtc cttggcgtcg tccgggccga ccgtgacgac ggtgacctcg
     2701 gcctcgtcgg cctcctcgga gatccgcagg gcctgctcga ccgcgtactc gtcgagctcc
     2761 gagaggaggc cgtcgacctc gtcgcggtcg acggtcaggt cgtccgcgaa tccgcggtca
     2821 ccggtggcgt cgggaacgta cttcacacag acagcgatcc tcagggtcac ggcctggctc
     2881 ctttcaacga gcggatggtt acgggaggtt gcgggccatg acgatgcgct ggacctggtt
     2941 ggtgccttcg tagatctggg tgatcttggc gtcgcgcatc atgcgctcga cggggtagtc
     3001 gcgggtgtag ccgtagccgc cgaggagctg gacggcgtcg gtggtgacct ccatggcgac
     3061 gtcggaggcg aagcacttgg ccgcggcgcc gaagaaggtc aggtcgccgt cgacgcgctc
     3121 ggacttcgcc gcggcggcgt aggtgagctg gcgggccgcc tccagcttca tggccatgtc
     3181 ggcgagcatg aactgcacgc cctggaagtc ggcgatcggc ttgccgaact gcttgcgctc
     3241 cttgacgtag cccttggcgt agtcgagggc gccctgcgcg atgcccagcg cctgcgcggc
     3301 gatggtgata cgggtgtggt ccagcgtctt catcgcggtc gcgaaaccgg tgccctcggc
     3361 gccgatcatc cggtcggcgg ggatgcggac gttgtcgaag tagacctcgc gggtcgggga
     3421 gcccttgatg ccgagcttct tctccggcgc gccgaacgac acgccctcgt cggacttctc
     3481 cacgacgaac gcggagatgc ccttcgagcg catcgaaggg tcggtgacgg ccatcaccgt
     3541 gtagtactcc gagacgccgg cgttggtgat ccagcgcttc acgccgttca gcacccagaa
     3601 gtcgccgtcg cgcaccgcgc gggtcttcat ccccgccgcg tccgaacccg cgtccggctc
     3661 cgacagcgcg taggagaaca tcgcctcacc cgcggcgagc ggggcgaggt acttggcctt
     3721 cagctcctcc gagcccgaca ggaccaccgg cagcgagccc agcttgttca ccgccgggat
     3781 cagcgacgac gaaccgcaga cgcgcgcgac ctcctcgatc acgatcaccg tcgccagcgc
     3841 gtccgcgccc gcacccccgt acgactccgg cacgtggacc gcgtgcaggt cgttcgcgac
     3901 cagcgcgtcc agggcctcct gcgggaaacg cccctcctcg tccaccgccg ccgcgaacgg
     3961 agcgatcttc gcctccgcca gcgcacgcac cgactcccgg agcatctcgt gctcctccga
     4021 cgtccggtac aggtcgaagt ccttgcccat gctcttgagc tccctggaat gcttgtggca
     4081 ctgagtaccc tcagcaaaac acactcagtg ccatgatgcc agagcgcccc gacggcggag
     4141 tgccgtcggg gcgcataaaa gaccggtcgg tatgtttttg ttgggacgct ccggttacgc
     4201 gtggacgtcg gaagagacgt cgtcccgatg ctggcgcccg gcctcgtact cggccgcgag
     4261 cgcgcgcccc cgctcggcgc tcatgtccag ttcgagcagg atcgacggcg tgcatatcga
     4321 cgggatcaga tgcttcatca gcaccgtcgt ccgatgcggg agatcattgc gatcgcacag
     4381 caccgcggac atcgtctgga ttccggtgaa cgcgccggag aacagctcgg ccgtgtccgt
     4441 cacgttcaca tggcggagca gctcgccgcg cgccctcgcc acggtgagga tgtccgagac
     4501 ctggtcgacc caggcctgga acgggatggc acggttgacg ctcaccgccc cgctgtcgag
     4561 ggcgagcgcg acgctcgcct gcaccagcgg gtcgtgctgg aggcggtggg cgagcaggaa
     4621 gccctggtct atgagctcct ggagcttgac ctcctgcggg gtgagcggct cgtcgagcag
     4681 ctgggcgtcg aggacgccca tggcgacctc ttccttggat gcaaagtgga aatacagcgc
     4741 acccttggtc actccggcgc gcgcgaggat ctccccgacg gtggcccgct cgtatccgcg
     4801 ctccgcgaag acggtggcgg ctgcttcgag gatcgtccgt cgtgttcgga tggcacgcgc
     4861 ttgttgtgcc acagggccct cccgctgtct cactgtgtgc tttcgccgtg tgacgtgaag
     4921 cattgaaaaa gaaaccggaa cgtacgtacc ctacgccggg ccgggcatga ggctcggggg
     4981 gaaggccaca gggggaagac atggaccggc acgaaccaca gggggaaacg aaagggacga
     5041 gcgtccccgg agagttcgtc caccggcacg accccctgga catcatcccc accggctgga
     5101 cgcggctcgc ggagaaccgc ttctccgtgt ccgccgcctg gtccaccggg catcccttct
     5161 tctccccggt cgatggcgac cggcacgatc cgattctggt cgccgagacc atacgccagg
     5221 cgacgatgct catcgcccac gccgagtacg acgtccccgt cgagtcgcag ttcgtcctct
     5281 gggacctctc ctacgacgcc gagcccgacg ccctcctggt cgacgacgac ggcgcggacc
     5341 tcaccgtcga cgtgatcctg tccgacgtac gaggccgggg ccgggggctg cgggcgctgc
     5401 gcaccaccac ggtcctcacc cgggacggac gcctcgtggc gaccggccgc ggcgtggcga
     5461 gctgcacctc cgccgccgcg taccgccgga tccgcggccc ccagctggcc ctcctcggcc
     5521 ggccggtgcc gctcataacc ggcgtcgccc cccactcggt gggccgggag cggcccgagg
     5581 acgtggtcct cggggcgggc gccctgcccg gccagtggca gctgcggatc gacaccggcc
     5641 accccacgct cttccgccgc cccaacgacc acgtccccgg catgctgctc ctggaggcgg
     5701 cccgccaggc ggcgcacgcc acgttcgggt ccgagaagtt cttcccctcg gtgatgaacg
     5761 tctccttcca ccggtacgcc gagctcgaca ccccctgctg gatcaccgcc gaggtcgtcc
     5821 ccgccctgga ccccgagacg tacgccgtgc ggatctccgg acgccaggac ggccagtccg
     5881 tcttcacctg caccttcacc tcgccgaccg gcgccctcgc ccccgtcgag gtcgaccacc
     5941 gcctcgcgag ctgaccgccc ggccgcgccc gcccctctca ccggaagaac gtcatgggaa
     6001 cccgcctcct cgtcaccggc gccaccggct tcatcggcgg ccgggtcgcc gccgcggccg
     6061 ccgcgcaccc cgatctcgac cgggtgcgcc tgctcgtcag gaacccggac gcggcccccg
     6121 ccgcgcacca ggacgccccc gccggcgggc ccgtcgtcga gaccgtccag ggcgacgtcc
     6181 gcgaccccgc gagcctgcgc cgcgcctgcg aaggggtcga cgccgtcatc cactgcgcct
     6241 ccgcgatcgg cggcgaggag gagctcctgc gggccgtcaa cgagcacggc acccgcgacc
     6301 tcgtcgacgc cgcccgccgg gccggagccg gccggatcgt cgccctcagc accgcctccg
     6361 tccacggccg cggccccttc cgggccgccg cgcccggcac cctcccgctc gccccggtgt
     6421 cggcgaccag ccgtacccgc gccgccgggg agcggtacgt cctggacgcc ggcggcaccg
     6481 tgctgcgccc gcacctggtc tacgggacgg gggaccggca ggtcgtgccc gggatcctga
     6541 cgctgctcgc cgcgctgccc gggccgctgg ccggcagcgg ctcgctccac tcgctcatcg
     6601 aggtggagag cctggcgggc gccctcctgg gcgccgccct ctccccggcg accgggccgg
     6661 gcgcgtacta cctcgcccat ccggacccgg tctccgacga cgagctgctc gccgccgtcg
     6721 agcccctgct cccggagtcg gcgagggccg cccgggggcc cgccgtggag ttcgccgagg
     6781 cccggaagct gctggtcggg aacccgctcg ccgcccacca cctggagatg ttcggcctcg
     6841 accactggtt cgccgacgag cgcccctgga ccgacttcgg ccgcggcccc ggccccggtt
     6901 tcaccgccgt cttcccccgc cacctgagct ggtaccggcg acttctcgcc gaccgcgcgg
     6961 aacgggcata gtccgtcacc tcggcgaccg caccgcgcgt ctaaggtggg ggcacgatga
     7021 ccagtttccg tgagagtgtt cggaccctgc tccgggagca ggtgctcgat gccgcctatg
     7081 ggctcgtggc ggccgacgga tggggcgtgc tgcggatgac cgccatcgcg cgggccgcgg
     7141 ggatcagccg gcagacgctc tacaacgagt tcgggtcgaa ggaggcgatc ggcggggcgc
     7201 tcgtgcggcg cgagctggag cggttcctgg tggggatcca gcgcgagctg gacgcgcacc
     7261 ggggacgcct tgagcgggcc gcgcgggccg gggtggagta cacgctccag caggccgtcg
     7321 acaatccgct gatcaagagc gtgctgctgg ccgcccgggg cggggccgac gccgatctgc
     7381 tcgcctatct gaccacgcgc accgagcccg tcttcgggac cgcgatgacc ctgctcgacg
     7441 cgtacgcgat cgaggcctgg cccgacgtgg acgaggagtc gcgggggctc gccgtcgaga
     7501 ccatcgtgcg gctcaccgtc agccacatcg tccagcccgt cgcgccgccc gaggagtccg
     7561 cgcggcggat cgcccgcgtc accgcgcggg tcgcctaccc gggcggggtg ccggaggact
     7621 gaccgggccg gtcagtcccc ggtcaggggg accgccgccg acaggaccgt gccctcgccc
     7681 ggggccgact cgaccgtgag ggtgccgccc agctggcggg cccgggtgcg gatcgccgga
     7741 aggccgtggc cccggacgcc gtccccgtcg cgggcggccg acgggtcgaa gccccggccg
     7801 tcgtcggcga tgtccaggac gacctggtcg tcgaggtacg tgagggtgag cgcggcggta
     7861 cgggcctccg cgtgctcccg tacgttggcg agcgcgccct gcgcgatccg gagcagcgcc
     7921 gactgcaccc ggtccgggag ccggcggccc tggccgccct cgacgtggca gcggacggcg
     7981 aggccgtccg tggactcgcg gcgggccagg gtgcgcaggg cctcgtccag gccgccgccc
     8041 ccggcgaggt cggcgggggc caggtcgtgg acgaagcgac gggcctcggt gaggttgtgc
     8101 tcggcgatcg cggtggccgc gcgcacgtgc cggtgggccg tgtcggggtc cgaccgccag
     8161 gtgcggtcgg cggcctggag cagcatctgc tggctggaca ggccctgggc gagggtgtcg
     8221 tggatctcca tggagagccg ctgccgctcg gcgagggtgc cctcgcgccg ctcggtggcg
     8281 gcgagctccc tgcgggtgcg gacgaggtcc gtgatgagct cgcgctgccg ccgggtctgc
     8341 tcgtccatgc ggacgaagac ggcgacgcag atcgccgcga ccgccggcgg accgagcagg
     8401 atgtcgaact cgaagcggcc ggcgaggcgc agctgcgcgg tgaccgagac gaccgtgagc
     8461 agcccgatga aacaggccgc gaccggcgga cgcagattgc gcaggccgag gtagaagagc
     8521 gggatcgagc accaggcgaa gctcggggcg aggaagacca gcgcgatcca cgtcgccgtg
     8581 caggcaccga gccacaggtg gcgccggctc cggtcggcgg cgagcgcggg ctcggccacg
     8641 tacagcaccg cgagtccggc ggcgagggcg accacccagg gggcgatccc ctcgccggtg
     8701 tgccgcatca ggaagcgggt cagcgaggag ccgagcagca ggaagaagcc cgtgtggagc
     8761 aggacccgga accagcgggc ctcgtcgtcc gccgaccgcg cgtcccctcc ggggcccgtc
     8821 gccgtccccg tcccgtccca ccgtgccatg ccgtgccgta cctctccgcc gcccgccgat
     8881 cggatgaacg gagcaaacct ctcatatccg ccgcgcagga cgagcacggt cgtggcggcg
     8941 ggggagagcg gtcggccgcg agggtctgcc cctgcccgtg tcggacaggc cctcccgcat
     9001 ccgcgccggc gcgtctggga accgtcaaga tcccacgggc ggccgtcggc cgtcggccgt
     9061 ctcgcaccgg tacggctaca gccagccgtt gcgccggaag ccgcggtgga tgacgtaaca
     9121 ggccaccgcg atcaccccga ggaccaccgg gtagccgtag gtccagccca gctccggcat
     9181 gtggtcgaag ttcatgccgt agaccccgca gaccatggtc ggcacggcga cgatcgccgc
     9241 ccacgcggtg atcttccgca tgtcctcgtt ctgcgccacg gtcacctgcg ccaggtgcgc
     9301 ctgcagcacg gagtcgagca gggtgtcgta cgaggcgatc tgctcggtca cgcgggagag
     9361 gtggtcggtg acgtcccgga agtacgcccg gatctccggg tccaccagcg gcatcggccg
     9421 ggtcgcgagc gccgtcagcg ggcgggcgag cggtgcgacg gcccggcgca gttcgagcag
     9481 ctcccgtttg agctggtaga tccggcccgc gtcgcggcgg tcggcctgcg cggagaagac
     9541 cgcgttctcg atcgcgtcga ggtcggtctg caccgcgtcc gtgacggcga ggtagtcgtc
     9601 caccacgtgg tcgacgacgg cgtgcagcac cgccgacggg cccttgacca gctgctcggg
     9661 cagctcctcc agggcctcgc gcagggggcc gagggagccg tgcccgccgt gccggacggt
     9721 gatgacgaag tcgggtccga cgaaggccat cagctcgccg gtgtccacca cctcgctggt
     9781 cgcggtgagt tcctcgtgct cgacgtaccg gaccgtcttc aggacggcga agagcacgcc
     9841 gtcgtactcc tcgatcttgg ggcgctgatg ggcgtggacg gcgtcctcga cggccagcgg
     9901 gtgcagtccg aagagctcgg cgaggacggt caactccttc tccgccggct cgtgcaggcc
     9961 gatccacacg aagccgtcgc cggtgcgccg cacatgccgc agcgcctcgt ccgcgccgcc
    10021 gccccggacg gtgcggcgca cgccgtcggc gtacaccacg cagttgacga ccgcgctgcc
    10081 gagcggcgag cgcgccgggt ggctcaggtc cacggtgcgg cgataggtcc ggcggacggc
    10141 ggtgcgcagg cgctggatca cggacacggt ggtactcctt cggcagaccg ggccagtgtg
    10201 ccaccgcccg gcgccgaggt cagcctgccg ccggaggcgc cccgaggacg cggtcgaggt
    10261 acggattgga gaggacgccg gaggggtcga gggagttccg gagggccacg aagtcgtcga
    10321 agtgctcgta gcgggggtgc agttccgccg cgccgaggtc gtgcatcttg ccccagtgcg
    10381 ggcggccgcc gtaggagccc aggatccgct ggcaggcgtc gaagtaccgg cggtacggca
    10441 tccggtggta ctggtggaag gcgatgtagc agctctcccg gccctgtgcg gtggagagcc
    10501 agatgtcgtc ggcggcggtg aagcggacct ccagcgggaa cgacaccggc tccccgtggg
    10561 cgtcgatcca cttccgcagc tcgcgcagcg cctccgccgc gtgggcgcgg gggatcgcga
    10621 actcgccctc gtggaagcgg acgtcgcgga ccgaggcgaa gatccggtac gcgtggtccg
    10681 tccaggtccg ggaggacatc gcgcgcgcgg cgaagcgggt gatcggcggc accaggcggg
    10741 gcaccagggt gccgagccgg ttcagcgcct cgaaggcggg accgctcacg gcctcgtcca
    10801 ggcgccggga gaggtcgccg aggggggaga ggggggtgtc gccgggcagt cggcggaagc
    10861 ggcgggtgag cgtggtctcg ctgtgcggga accagaagaa ctcgaagtgg tcgttgtccg
    10921 cgacgagttc gtcgagccgg ccgagggtct ccgc
//
