LOCUS       NZ_JABBGE010000004     11632 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. R302 contig4, whole genome shotgun sequence.
ACCESSION   NZ_JABBGE010000004
VERSION     NZ_JABBGE010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. R302
  ORGANISM  Streptomyces sp. R302
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 676282)
  AUTHORS   Dahal,R.H.
  TITLE     Streptomyces sp. R302 sp. nov., isolated from soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 676282)
  AUTHORS   Dahal,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) Life Science, Kyonggi University, Kyonggi
            University, Suwon, Gyeonggi-Do 16227, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2020
            Assembly Method                   :: SPAdes v. APR-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012927245.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,357
            CDSs (total)                      :: 7,271
            Genes (coding)                    :: 7,075
            CDSs (with protein)               :: 7,075
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 164 of 196
            Pseudo Genes (internal stop)      :: 20 of 196
            Pseudo Genes (multiple problems)  :: 67 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 180886
            Orig. end                         :: 192518
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBGE010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11632
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11632
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11632
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6632](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6632)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    12..32
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     misc_feature    complement(199..219)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 17.66"
                     /tool="antismash"
     gene            778..2289
                     /gene="hemG"
                     /locus_tag="HHL19_RS13795"
                     /old_locus_tag="HHL19_13800"
     CDS             778..2289
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847917.1"
                     /locus_tag="HHL19_RS13795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13800"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_169089230.1"
                     /sec_met_domain="DAO (E-value: 1.8e-11, bitscore: 39.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNADTTGPSSGHRTGHRAGHPDGPHAVVVGGGITGLAAAHRLLAA
                     GARVTLLEAGGRLGGKLLAGEIAGAPVDLGAESLLARRPEAIALAEAVGLGDRLRAPAT
                     TTASVWSRGRLVPMPKGHVMGVPGDAEAVAGLLSEEALRRIGRDSELPPTELGEDVAIG
                     AYVAERMGHEVVDRLVEPLLGGVYAGDAYRISMKAAVPALFEAARRHPSLTAAVRAVQA
                     AGSALPADVAGAATGLGGSVFAGISGGVGTLPGAVADAVRAAGGTILTGTPATALHRSG
                     ATGWRVETPERTLDADAVVLAAPAPVAAGLLGGHAPGAAALLGTIEYASMALVTLAFRR
                     ADVPELPGSGFLVPPVDGHTIKASTFSSRKWGWVGEAAPELFVLRTSVGRYGEESQLLR
                     DDEDLVAASLKDLGAATGLATRPVATTVTRWTGGLPQYPVGHLDRVARARAALADLPGV
                     RLAGAAYDGVGIPACVASAHRAADALADEIIATSETGHPGAGHSS"
     gene            2307..3020
                     /gene="hemQ"
                     /locus_tag="HHL19_RS13800"
                     /old_locus_tag="HHL19_13805"
     CDS             2307..3020
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="HHL19_RS13800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13805"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_169089232.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPADRSGYADE
                     VQELFDRLAEKDITIRGTYDLSGLRADADVMIWWHAETSDQLQEAYNLFRRTKLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
     gene            complement(3184..3912)
                     /locus_tag="HHL19_RS13805"
                     /old_locus_tag="HHL19_13810"
     CDS             complement(3184..3912)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055540.1"
                     /locus_tag="HHL19_RS13805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13810"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_169089234.1"
                     /transl_table=11
                     /translation="MVWIPLLLVAGVVTAVACARLCRASIAAGGSAAGAVGDGGLTLGE
                     AAFLAGGPGRVAELTLVSMHRARRLLLARTGWATVVDGSDGGDELERAVLGAIGPEGQA
                     PVPGLRPAVAAAACVRRIEEGLVERGLAVPAPGRREVAAGLRAVRGAFLLALALAAVAV
                     PLVPEAERARVIAGFLLPLTTSGFCLLIGRAESYPRSPWASHAGQRLLAGLSAEDPLTA
                     LATRGPAVLEPELRAALGGP"
     gene            complement(3958..4995)
                     /locus_tag="HHL19_RS13810"
                     /old_locus_tag="HHL19_13815"
     CDS             complement(3958..4995)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HHL19_RS13810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13815"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_169089236.1"
                     /transl_table=11
                     /translation="MDPFLLALHLVAFVLLARLWVLARRTRLGPGGPVMDVYEAAFLGG
                     GPGRVADTALAAMHLDGRIVIGGPGVVAVVHRIAHDPVERAVLDVHAAAPSGALHQLRL
                     GVMTHPAVQEIGDGLAARGLVTLPGPRRAVRRWCVGLMLGAFLLFPLGIVGTVTGFALD
                     GPSVPFFVKILPLFFVCFATALVCGSAYSRQVSPAGTAALAAYRREHGTGGVHAVALGG
                     IRALSDRELWERLRDASRDGRMLSGGRSGTSSRSSYSSHSSHSSHSTAGAAVVMWCAGA
                     DMGGGDGWGSDSGSGSGSGGGSGCGSGGSSCSSSSSSCSSSSSSCSSSSSSCGSSSSSS
                     CGSSG"
     gene            complement(5001..6632)
                     /locus_tag="HHL19_RS13815"
                     /old_locus_tag="HHL19_13820"
     CDS             complement(5001..6632)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491673.1"
                     /locus_tag="HHL19_RS13815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13820"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_169089238.1"
                     /sec_met_domain="DUF692 (E-value: 2.3e-93, bitscore: 307.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTELGIGIGWRPEIADAVEELPGVDWVEVVAENVCADHLPDSLAR
                     LRARGVTVVPHGVSLGLGGADRPDPERLAALAAKAEALGAPLVTEHIAFVRAGGPLTAT
                     PRLEAGHLLPVPRTREALDVLCENVRIAQESLPVPLALENIAALFSWPDEELTEGRFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPARALAELPVEALAYVHVAGGLERDGVWHDTH
                     AHPVPQPVLDILADLAGRIAPPGVLLERDDHFPPATELAAELSTIRTTLTTAPRRRPAP
                     TTEDAAAPQAADETAADTLTAGGAGDVHAARAAAGPLAARHGVGTAALRAGRALTGGAP
                     LHIGAGARVSGGPMPSTHPGPTPTPTPTPTPITAADHAPAPSGGPVATLPRPDAETGTG
                     PKPGTGTGTGTEAEGAVRQRVGVAQAAVLSALVAGTPVPEGFDVRRMRVQGRALAAKRA
                     SVVARIAPELPEILGDGFRPAFLAYARSRPLPGNYRRDALDFVERLLLEDRPERRELTR
                     WWRERAESRPVGGFVGRLARRLLGGR"
     gene            complement(6714..7421)
                     /locus_tag="HHL19_RS13820"
                     /old_locus_tag="HHL19_13825"
     CDS             complement(6714..7421)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037038.1"
                     /locus_tag="HHL19_RS13820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13825"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_346768335.1"
                     /transl_table=11
                     /translation="MSSNDTDPRDEKPQFVLPLVVRIERDAPPARTDALQTAARAVLSL
                     LTDERSLGDGEWATAVADWEDARIRKVVRRARGAEWRRAEALPGITVTGETAEVRVFPP
                     VPLDGWPKELAKLQVSGTDLDDPAAPGPVPEGAAVLWMNPGLGMSAGKAMAQAGHGAQL
                     LWWALDDDARAAWREAGFPLAVRTAPEAEWPALAASGLPVVRDAGFTEIAPGSATVVSA
                     YPGVLAVAPGNLK"
     gene            7495..>8091
                     /locus_tag="HHL19_RS13825"
                     /old_locus_tag="HHL19_13830"
                     /pseudo=""
     CDS             7495..>8091
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="HHL19_RS13825"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13830"
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSTIVGAREPVRLTGVGRRYGPRGPWVLRGVDAELPAGRLVRVTG
                     ANGTGKSTLLRLVAGVDAPAEGRITGRPARTAYVPERFPPALPFTALDYLVHLGRLQGL
                     RTAPARARAGEWLARFGADAYAGTPLAALSKGTAQKVAVAQALLADPELLVLDEAWTGL
                     DPEARAELDAAVRERVAAGATALFVDHDPRRLAGDP"
     gene            8829..9671
                     /locus_tag="HHL19_RS13830"
                     /old_locus_tag="HHL19_13835"
     CDS             8829..9671
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHL19_RS13830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HHL19_13835"
                     /product="ABC transporter"
                     /protein_id="WP_206324982.1"
                     /transl_table=11
                     /translation="MTALLRYHSALFLRSQRWLAPLLLYAALVAVGVRPGDPVLGSLGI
                     AAAGLVPVAAWSVRICLTQEEAAARAVTAAAGGRNRAHLAALLTGAGGPVLVGAVAVGV
                     VTGTGDLAGTGGAAALLAGLPATTACALTGAAVGALASRPFVKAPGWSVAGLLLGSLLA
                     LVTTGSPAKHAVTALVASAGAPWGACAAAVLLAAATAAAAVRAAAPGVRRSSLGVMTTE
                     SAAYCAPEPAPRPPKAAEGPYARCVLCLEPTEYAESVKGIVLCPVCEWQEAQRTACSG"
     gene            complement(9684..10580)
                     /locus_tag="HHL19_RS13840"
                     /old_locus_tag="HHL19_13845"
     CDS             complement(9684..10580)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890194.1"
                     /locus_tag="HHL19_RS13840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13845"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_346768336.1"
                     /transl_table=11
                     /translation="MTISVRKVAALGVLGAVLVGCGGGADTPAAGPTTAAASPTKPTAA
                     PGAKPAPAAKPSPAARPAAPPTLAAGPQGRTPVYERRTGRSAEKVVALTFDADMTADQG
                     PRAARGERFDNPELIATLRRLKVDATIFMTGRWAEEYPDQARSIGTDPRFEIANHSYSH
                     YAFAEPCYGLPAVAEPDMAADVKRAFDSFRNAGAVNVVPYFRFPGGCYDDAALHALAPA
                     RVTAVQWDVVGGDAFAKDPDAVAEQVLSGVRPGSVVVLHCTRSAAPATERAMRRVVPEL
                     RARGYRFVKVSELMREG"
     misc_feature    complement(10560..10573)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.01"
                     /tool="antismash"
     gene            complement(10652..11500)
                     /locus_tag="HHL19_RS13845"
                     /old_locus_tag="HHL19_13850"
     CDS             complement(10652..11500)
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063346.1"
                     /locus_tag="HHL19_RS13845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HHL19_13850"
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="WP_206325015.1"
                     /transl_table=11
                     /translation="MSAALRLPPGERVDLSAYDTRATPGGPGGEDGPGGKTAGRAATAL
                     LAPRLAALQERLYAASTAGDRRRLLLVLQGMDTSGKGGTVKHVIGHFNPSGCRIRAFKA
                     PTEEERAHPFLWRVTRALPRPGEIGIFDRSHYEDVLIARVRELVPRAEVERRYEEIDRF
                     ERALAEDGVTLVKVFLHLSYEEQRARLLERLDNPEKHWKFDPADIEERTRWPAYQEAYE
                     LALERCSSPEAPWYVVPADRKWYRNWAVSTLLLEHLEALDPRYPRAGFDVAGCRRRLLA
                     T"
ORIGIN
        1 ccagcagcgc ctccaggtcc gcctgacgac gactcagctc cgactccagg gccaccacgt
       61 cggccagctt gtccgcccgg tccatcagcg cccgcacccg cgcgacgctc gcccgctggg
      121 tcgcgatccg gctctccacg tcgacgacct gatcggtgac gtcctgcgcc tccgccgtgc
      181 gggagagcac cttcccggta cccgtcaggt cgccgaggac ccggtcgtac cggtcctgcg
      241 gcacccgcag caccatccgg gaggcctcgt accgctcgtc ggtccgctcc gtcgtctccg
      301 actcgacccg gccgcccgcc gccgcgacca ccgcccgtac ccggtcggcg gtcgccgcca
      361 cccgctcgac ctccaccgtg agggaggccg tccggacgag gtgctgcacg gcgggcgcct
      421 tcgacgcccc gggcttcgcg gcggcggcct ccgagccgcc cgcgcccgtg ccgcccttcc
      481 ggtccgtgcc cgcgggcgcg gccgcgtccg ccgccacgcc cttgccgtcg gccgcgtcgg
      541 tgccgccgcc cgcgcagccc gccgtcagcg ccagcgccgc ggccagcagc agcgccgccc
      601 ccgccctgct ccgtccgccc gttccgtccg ccttgtgcac cgtgccccct ggggctcgca
      661 ggaatcgatg ctcctaggac gtgcgggacc ggcgcgcggt ttgcctccgc cggtagcgat
      721 gcggtcacgg tccggcctcg gaacgggggc gagcgggcga tttgagagag tggtcccatg
      781 aacgcggaca ccacgggacc ttcctccgga caccggaccg ggcatcgggc cggacacccc
      841 gacggacctc acgccgtcgt cgtcggcgga ggcatcaccg gcctcgccgc cgcccaccgg
      901 ctgctcgccg ccggtgcgcg cgtcaccctc ctggaggccg gcggccggct cggcggcaag
      961 ctgctcgccg gggagatcgc cggcgccccc gtggacctcg gcgccgagtc cctgctcgcc
     1021 cgccgccccg aggcgatcgc cctcgccgag gccgtcggcc tcggcgaccg gctccgggcg
     1081 cctgccacca ccaccgcctc cgtctggtcc cgaggccggc tcgtccccat gcccaagggc
     1141 cacgtcatgg gggttcccgg cgatgccgag gccgtcgccg ggctcctctc cgaggaggcc
     1201 ctgcgccgga tcggccggga ctccgagctg ccccccaccg agctcggcga ggacgtcgcc
     1261 atcggcgcgt acgtcgccga gcgcatgggc cacgaggtcg tcgaccggct cgtcgagccc
     1321 ctcctcggcg gcgtctacgc cggcgacgcc taccggatct cgatgaaggc cgccgtcccc
     1381 gcgctcttcg aggccgcccg ccgccacccc agcctcaccg ccgccgtccg cgccgtccag
     1441 gccgcgggca gtgccctccc cgccgacgtc gcgggcgccg ccaccggcct cggcgggtcc
     1501 gtcttcgccg gcatctccgg cggcgtcggc accctccccg gggccgtcgc cgacgccgtc
     1561 cgcgccgccg gcggcaccat cctcaccggc accccggcga ccgccctgca ccggtcgggc
     1621 gccaccggct ggcgggtgga gaccccggag cggaccctcg acgcggacgc cgtcgtcctc
     1681 gccgcccccg cgcccgtcgc cgccgggctc ctcggcggcc acgcccccgg ggccgccgcg
     1741 ctcctcggca cgatcgagta cgcctccatg gccctggtca cgctcgcctt ccgccgcgcc
     1801 gacgtccccg agctgcccgg ctccggcttc ctcgtgccgc cggtcgacgg ccacaccatc
     1861 aaggcctcca ccttctccag ccggaagtgg ggctgggtcg gcgaggccgc ccccgagctg
     1921 ttcgtgctcc gcacctccgt cggccggtac ggcgaggagt cccagctcct ccgcgacgac
     1981 gaggacctgg tcgccgcctc cctcaaggac ctcggcgccg ccaccggcct cgccacccgg
     2041 cccgtcgcca ccaccgtgac ccgctggacc ggcggcctgc cgcagtaccc ggtcggccac
     2101 ctggaccggg tcgcccgggc ccgcgccgcc ctcgcggacc tgcccggagt gcgcctcgcg
     2161 ggcgccgcgt acgacggcgt cggcatcccc gcctgcgtcg cctccgccca ccgcgcggcc
     2221 gacgccctgg ccgacgagat catcgccacg tcggaaacgg ggcaccccgg tgcggggcac
     2281 tcctcatgac gggcgagaat aggcgtatga gtgcgcccga aaagatcccg aacgccggta
     2341 agaaggcgaa ggacctcaac gaggtcatcc gctacaccct gtggtctgtc ttcaagctgc
     2401 gcgacgtcct gcccgccgac cggagcggct acgccgacga ggtccaggag ctcttcgacc
     2461 ggctcgcgga gaaggacatc accatccggg gcacgtacga cctgtccggt ctgcgcgccg
     2521 acgccgacgt catgatctgg tggcacgccg agacgagcga ccagctccag gaggcgtaca
     2581 acctcttccg ccgtacgaag ctgggccgcg ccctggagcc ggtctggtcg aacatggcgc
     2641 tgcaccgccc ggccgagttc aacaagtcgc acatcccggc cttcctggcc gacgagaccc
     2701 cgcgcgacta cgtctcggtc taccccttcg tccgctccta cgactggtac ctgctgccgg
     2761 acgaggaccg ccgccggatg ctcgccgacc acggcaagat ggcccgcggc tacccggacg
     2821 tccgggccaa cacggtcgcc tccttctcgc tcggcgacta cgagtggctc ctcgccttcg
     2881 aggccgacga gctgtaccgc atcgtcgacc tcatgcgcca cctgcgcgcc tccgaggccc
     2941 ggatgcacgt ccgcgaggag gtccccttct acaccggccg ccgcaagagc gtcgcggacc
     3001 tggtggcggg cctggcctga cggccccccc gaacctggcg gcgccccggc tcccagccgg
     3061 ggcgttcgcc cctcgacccg gaagatcaga cggccgccac gccatggccg gccgctccag
     3121 cgacaccggg tccggcaccg ggtgcggcgc gcgcgagcgc gccgcaccgg cgtcccttcc
     3181 ctctcagggc ccgcccagcg cggcccgcag ctccggctcc aggaccgccg gcccccgggt
     3241 ggcgagcgcg gtgagcggat cctccgccga gaggcccgcg agcagccgct gaccggcgtg
     3301 cgaggcccag ggactgcgcg gatacgactc cgcccgcccg atcagcagac agaaccccga
     3361 ggtcgtcagc ggcagcagga acccggcgat cacccgcgcc cgctccgcct ccggcaccag
     3421 cggcaccgcc accgccgcca gcgcgagcgc cagcaggaac gcaccccgca ccgcccgcag
     3481 cccggccgcg acctcccgcc gccccggcgc cggcaccgcg agcccccgct ccaccagacc
     3541 ctcctcgatc cggcgtacgc acgcggcggc ggcgaccgcc ggacgcagcc ccggcaccgg
     3601 cgcctgcccc tccgggccga tcgcgccgag caccgcccgc tccagctcgt ccccgccgtc
     3661 cgagccgtcc acgaccgtgg cccagccggt gcgcgcgagc agcagccgcc gggcccggtg
     3721 catcgacacc agcgtcagct cggcgacccg cccgggaccg ccggcgagga acgcggcctc
     3781 gcccagcgtc agtccgccgt ccccgaccgc ccccgcggcg gacccgccgg cggcgatcga
     3841 ggcccggcag agccgggcgc aggcgacggc cgtcacgacg ccggcgacga ggagcagcgg
     3901 gatccacacc atgatcgagt tgtacgtcag gacccccgcc cccgccaccc gaacggctca
     3961 gccgctggac ccgcacgagg acgacgacga ggagccgcag gacgaggagg acgacgaaca
     4021 ggacgaggac gacgacgaac aggacgagga ggacgacgag caggacgacc cgcccgaccc
     4081 gcaccccgag cccccgccgg acccggaccc ggaccccgag tccgaacccc agccgtcccc
     4141 gccgcccatg tccgcgcccg cgcaccacat caccacggcc gcgccggcgg tggagtgcga
     4201 ggagtgcgag gagtgcgagg agtacgagga ccgggaggag gtaccgctcc ggccgccgga
     4261 cagcatccgg ccgtcccggg aggcgtcccg cagccgctcc cacagctcgc ggtcggacag
     4321 cgcccggatc ccgccgaggg cgacggcgtg gacgcccccg gtgccgtgct cgcgccggta
     4381 ggcggcgagc gcggcggtac ccgccgggct cacctgccgg ctgtaggcgg agccgcagac
     4441 cagcgcggtg gcgaagcaga cgaagaacag cggcaggatc ttcacgaaga acggcaccga
     4501 gggcccgtcc agggcgaacc cggtgaccgt ccccacgatg cccagcggga agagcaggaa
     4561 ggcacccagc atcaggccga cgcaccaccg tcgcacggcg cggcgcgggc ccggcagggt
     4621 caccaggccg cgcgcggcga ggccgtcgcc gatctcctgc acggccgggt gggtcatcac
     4681 cccgagccgc agctggtgca gggcgccgct cggggccgcc gcgtggacgt cgagcacggc
     4741 gcgctccacc gggtcgtggg cgatccggtg gacgacggcg accacgccgg gaccgccgat
     4801 gacgatccgg ccgtcgaggt gcatggcggc gagcgccgtg tcggcgaccc ggccggggcc
     4861 gccgccgagg aacgcggcct cgtagacgtc catgaccggt ccgccgggac cgagccgggt
     4921 gcggcgggcc agcacccaca gccgggccag caggacgaag gcgacgaggt ggagcgccag
     4981 gaggaacggg tccatgccgg tcaccgcccc ccgaggaggc gccgcgccag ccgccccacg
     5041 aacccgccga ccggccggct ctcggcccgc tcccgccacc agcgggtcag ctcccgccgc
     5101 tcgggccggt cctccaggag cagccgctcg acgaagtcga gcgcgtcccg ccggtagttg
     5161 cccggcaggg gccggctcct ggcgtacgcg aggaacgcgg gccggaaccc gtcgccgagg
     5221 atctccggca gctccggcgc gatccgcgcc acgacggacg cccgcttggc ggccagcgcc
     5281 cgcccctgca cccgcatccg ccgcacatcg aacccctcgg gcaccggagt ccccgcaacc
     5341 aacgccgaca acacagccgc ctgcgccacc ccgacccgct gccgcaccgc accctcggcc
     5401 tcggtcccgg tcccggtccc ggtcccgggc ttcggcccgg ttcccgtttc cgcgtccggg
     5461 cggggcaggg tggctaccgg gccgccggac ggggccggcg cgtggtcggc tgcggtgatc
     5521 ggtgtcggtg tcggtgtcgg tgtcggtgtc gggcctgggt gggtggaggg catcgggccg
     5581 cccgagacgc gggcgccggc gccgatgtgg agcggggcgc cgcccgtgag ggcgcggccc
     5641 gcgcgcaggg cggcggtgcc gacgccgtgg cgcgcggcga gcgggccggc cgccgcccgg
     5701 gcggcgtgga cgtcccccgc gcctccggcg gtgagggtgt cggctgcggt ctcgtcggcg
     5761 gcctgggggg ccgccgcgtc ttctgtggtg ggtgccgggc ggcggcgggg cgccgtcgtc
     5821 agggtcgtgc ggatcgtgga gagttccgcg gcgagttccg tcgcgggcgg gaagtggtcg
     5881 tcccgttcca ggaggacgcc cggcggggcg atccggccgg cgaggtcggc gaggatgtcc
     5941 aggaccggct gcgggaccgg gtgggcgtgg gtgtcgtgcc agacgccgtc ccgttcgagg
     6001 ccgccggcca cgtggacgta cgcgagggcc tcgacgggca gttcggcgag cgcccgcgcc
     6061 gggtcctcgc cgcggttgac gtggttggtg tggaggttgg cgacgtcgat gaggagccgt
     6121 acccccgtac gctccaccag ctccgccagg aaccggccct ccgtcagctc ctcgtccggc
     6181 caggagaaca gcgcggcgat gttctccagc gccagcggca ccggcagcga ctcctgcgcg
     6241 atccgcacgt tctcgcagag cacgtccagg gcctcccggg tacggggcac gggcagcagg
     6301 tgcccggcct ccagccgcgg ggtcgcggtc agcgggccgc cggcccggac gaacgcgatg
     6361 tgctcggtga cgagcggcgc acccagcgcc tccgccttcg cggcgagcgc ggccagccgc
     6421 tccgggtccg gccggtccgc cccgccgagc cccagcgaca ccccgtgcgg cacgaccgtg
     6481 acgccccggg cccgcagccg ggccagcgag tcgggcaggt ggtcggcgca cacgttctcc
     6541 gcgacgacct ccacccagtc gaccccgggc agctcctcga ccgcgtccgc gatctcggga
     6601 cgccagccga tgccgatgcc gagttccgtc atgtccccct ccttcggtcc tcagtcatcc
     6661 acgtgatgac cccgccggag ccggtcgaac ccaggagggg caggttcaga gcttcatttg
     6721 aggtttccgg gcgccacggc caggacaccg gggtacgccg acaccaccgt cgcgctgccc
     6781 ggcgcgatct ccgtgaagcc cgcgtccctg accaccggca ggccgctcgc ggcgagcgcg
     6841 ggccactccg cctccggcgc cgtgcgcacg gccagcggga agcccgcctc ccgccaggcg
     6901 gcgcgggcgt cgtcgtccag ggcccaccac agcagctggg cgccgtgccc ggcctgggcc
     6961 atcgccttgc cggccgacat gccgagcccc gggttcatcc acagcaccgc cgcgccttcc
     7021 ggcacgggtc ccggcgcggc cgggtcgtcg aggtcggtgc cggagacctg gagcttggcc
     7081 agctccttgg gccagccgtc cagggggacc ggcgggaaga cgcgtacctc cgcggtctcg
     7141 ccggtcaccg tgatgccggg cagggcctcg gcgcgccgcc actccgcgcc gcgggcccgc
     7201 cgtacgacct tgcggatgcg ggcgtcctcc cagtcggcga cggccgtcgc ccactcgccg
     7261 tcgccgagcg accgctcgtc ggtgaggagc gagagcacgg cgcgggccgc ggtctgcagg
     7321 gcgtcggtgc gggccggggg cgcgtcgcgc tcgatccgta ccaccagggg caggacgaac
     7381 tgcggcttct cgtcgcgggg atcggtgtcg ttgctgctca cccggcaagt ctgccaccgg
     7441 cccccgggag gcggatcttg cggcccggtc cggggccggg cgaggatccc ctccatgagc
     7501 acgatcgtcg gagcgcggga gccggtgcgg ctcacggggg tggggcgccg gtacgggccg
     7561 cgcggcccgt gggtgctgcg gggcgtcgac gcggagctgc ccgccggccg gctcgtccgg
     7621 gtcaccggcg cgaacggcac cggcaagtcg acgctgctgc gcctggtcgc cggtgtcgac
     7681 gcgcccgccg agggccggat caccggccgc ccggccagga ccgcgtacgt ccccgagcgc
     7741 ttcccgcccg ccctgccgtt caccgccctc gactacctgg tccacctggg ccgcctccag
     7801 ggcctgcgga cggccccggc ccgggcccgc gcgggggagt ggctggcccg cttcggcgcg
     7861 gacgcgtacg ccgggacccc gctggccgcc ctctccaagg gcaccgccca gaaggtggcc
     7921 gtcgcccagg ccctgctcgc ggaccccgaa ctcctcgtcc tggacgaggc ctggaccggc
     7981 ctcgaccccg aggcccgcgc ggaactcgac gcggcggtcc gcgagcgcgt cgcggccggc
     8041 gccaccgccc tcttcgtcga ccacgacccc cgccgcttgg cgggggaccc cgacacgacc
     8101 ctgaccgtcg cggagcgccg cgtccgcccg gacccgggcc gcctggcggg cgaccccggc
     8161 gcgcccctgt ccgtcgcgga gggccgcgtc tgcccggacc cgcgccgcct cgcgggcgaa
     8221 cccgacacga ccccgaccgt cgtggagggt cgcgaccagc tggacccggg ctgcttggtg
     8281 ggcgaccccg gtgcgcccct gtccgtcgtg gagggccgcg accacctgga cccgcgccgc
     8341 ttggtgggcg aacccgacac gaccccgacc gtcgtggagg gtcgcgacca cctggacccg
     8401 cgccgcttgg tgggcgaacc cgacacgacc ccgaccgtcg tggagggtcg cgaccacctg
     8461 gacccgggct gcctggtggg cgaccccggt gcgcccctgt ccgtcgcgga gggccgcgac
     8521 cacctgggcc cgcgccgcct cgcgggcgaa cccgacacga ccccgaccgt cgtggagggt
     8581 cgcgaccacc cgcacccgcc cggccacgct cccggccacg cccccgttcg cgtcgtcgcc
     8641 tccggtgccc gtcccctgcc cgaccccctt cccggcggcg ccaccagggc gccgcgtgcg
     8701 gccggcgggt ctgtggtgtt ccacgtcgcc gcgccgcact ccgacgacct gctgcgcgcc
     8761 ctgctcgccg ccgcctggca cgtccatcgc gtcgaacccg accgggccgg ggcggagggg
     8821 gaacgcccat gacggcgctg ctgcggtacc actccgccct gttcctgcgc tcccagcggt
     8881 ggctcgctcc gctgctcctg tacgcggcgc tggtcgcggt cggcgtccgg cccggggatc
     8941 ccgtcctcgg gtcgctcggg atcgcggcgg ccgggctcgt gccggtcgcg gcctggtcgg
     9001 tgcggatctg cctgacgcag gaggaggccg ccgcgcgggc ggtgaccgcc gcggccggcg
     9061 ggcggaaccg cgcgcacctg gccgccctgc tcaccggcgc cggggggccc gtcctcgtgg
     9121 gcgcggtcgc ggtcggcgtg gtgacgggca cgggtgacct cgccgggacg ggcggagcgg
     9181 ccgccctgct cgccgggctg ccggccacca ccgcctgcgc gctcaccggc gcggctgtcg
     9241 gggcgcttgc ctcgcggccg ttcgtgaagg cgccgggctg gtcggtggcc gggctgctgc
     9301 tcggctcgct gctcgccctg gtgaccaccg gctcgcccgc gaagcacgcg gtcaccgccc
     9361 tcgtcgcgtc cgccggagcg ccgtgggggg cctgcgccgc cgccgtcctc ctcgccgccg
     9421 cgacggcggc cgcggccgtg agagccgccg cgcctggagt gaggcggagt agcctcggtg
     9481 tcatgaccac ggaatccgcc gcgtactgcg cccccgagcc cgcgccccgg ccgcccaagg
     9541 ccgccgaggg gccgtacgcg cgctgcgtgc tgtgcctgga gccgaccgag tacgcggaat
     9601 cggtcaaggg gatcgtgctg tgcccggtct gcgagtggca ggaggcccag cgcaccgcct
     9661 gctccggctg aggcaccgcc ccgtcagcct tcccgcatca gctccgacac cttcacgaag
     9721 cggtagccgc gtgcccggag ttcggggacc acgcggcgca tggcccgctc ggtggccggg
     9781 gcggcgctgc gggtgcagtg caggacgacc acggagcccg ggcggacgcc ggagaggacc
     9841 tgctcggcca ccgcgtcggg gtccttcgcg aaggcgtcgc cgccgaccac gtcccactgc
     9901 acggcggtga cccgggcggg ggcgagggcg tgcagcgcgg cgtcgtcgta gcagccgccg
     9961 gggaagcgga agtacggcac gacgttgacg gccccggcgt tccggaagga gtcgaaggcg
    10021 cgcttcacgt cggccgccat gtccggctcg gcgaccgccg gcaggccgta gcagggctcg
    10081 gcgaaggcgt agtggctgta ggagtggttg gcgatctcga agcgggggtc ggtgccgatc
    10141 gagcgggcct ggtcggggta ctcctccgcc cagcggcccg tcatgaagat cgtggcgtcc
    10201 accttcaggc ggcgcagcgt ggcgatgagc tccgggttgt cgaagcgttc gccgcgcgcc
    10261 gcccgtgggc cctggtcggc ggtcatgtcg gcgtcgaagg tgagcgcgac gaccttctcg
    10321 gcgctccggc cggtccgccg ttcgtacacg ggggtacggc cctgggggcc ggccgccagg
    10381 gtgggcggcg cggcgggccg cgcggcgggc gagggcttcg cggcgggtgc gggcttcgcg
    10441 cccggcgcgg cggtcggctt cgtgggggag gcggccgcgg tcgtcggacc cgccgcgggg
    10501 gtgtcggcgc ccccgccgca gccgaccaga acggcgccca gcacaccgag cgccgccact
    10561 ttacgtacag aaatggtcac gaacggaagc tatccgatcg gactcggagg tccgtcgtca
    10621 cacccccgga agggcgccct cccgtcaccc gtcaggtcgc gagcaggcgc ctccggcacc
    10681 ccgccacgtc gaagccggcc cgcggatagc gcgggtccag cgcctccagg tgctccagga
    10741 gcagcgtgct caccgcccag ttccggtacc acttccggtc cgccgggacc acgtaccagg
    10801 gagcctccgg cgacgagcag cgctccaggg ccagctcgta cgcctcctgg taggcgggcc
    10861 agcgggtccg ctcctcgatg tcggccgggt cgaacttcca gtgcttctcc gggttgtcca
    10921 ggcgctccag gagccgcgcg cgctgctcct cgtacgagag gtgcaggaag accttcacga
    10981 gcgtcacccc gtcctcggcg agcgcccgct cgaagcggtc gatctcctcg tagcgccgct
    11041 ccacctccgc gcgcgggacc agttccctga cccgggcgat gagcacgtcc tcgtagtggg
    11101 agcggtcgaa gatgccgatc tcgccgggcc gcggcagcgc ccgggtgacc cgccacagga
    11161 acgggtgggc gcgctcctcc tcggtgggcg ccttgaaggc ccggatccgg cagccggacg
    11221 ggttgaagtg gccgatgacg tgcttgaccg tgccgccctt gccgctggtg tccatgccct
    11281 ggaggaccag cagcagccgc cttcggtcgc ccgccgtgct cgccgcgtac agccgctcct
    11341 ggagcgcggc gagccgcggc gcgaggagcg cggtggcggc gcgcccggcc gtcttgccgc
    11401 ccgggccgtc ctcgccgccc gggccgccgg gggtggcccg ggtgtcgtac gcggagaggt
    11461 ccacgcgctc gcccggcgga agccgcagcg ccgcgctcag gggtacggcc cggcccccgt
    11521 caccgtccgg tccgtgccgg gtcccgccgc gctcctccgc caccgtgcgt cccttccttc
    11581 ccgccgcgcc cggggtcagc gccacgggcc ggtgacggcg aacgtggtgc cg
//
