LOCUS       NZ_JABAOB010000006     10927 bp    DNA     linear   CON 23-OCT-2023
DEFINITION  Chitinophaga sp. Ak27 Ak27-scaffold_6_length_543828_cov_146.194,
            whole genome shotgun sequence.
ACCESSION   NZ_JABAOB010000006
VERSION     NZ_JABAOB010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga sp. Ak27
  ORGANISM  Chitinophaga sp. Ak27
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1  (bases 1 to 543828)
  AUTHORS   Yin,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2020) State Key Laboratory of Plant Genomics,
            Institute of Microbiology, Chinese Academy of Sciences, Beichen west
            road, Bejing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-AUG-2019
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 246.8x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/23/2023 02:22:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,312
            CDSs (total)                      :: 6,224
            Genes (coding)                    :: 6,211
            CDSs (with protein)               :: 6,211
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:12:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 98971
            Orig. end                         :: 109898
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABAOB010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10927
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10927
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10927
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5927](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5927)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(97..110)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: strong, score: 23.52"
                     /tool="antismash"
     gene            complement(366..641)
                     /locus_tag="HGH90_RS20090"
                     /old_locus_tag="HGH90_20090"
     CDS             complement(366..641)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGH90_RS20090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGH90_20090"
                     /product="hypothetical protein"
                     /protein_id="WP_145661455.1"
                     /transl_table=11
                     /translation="MSKESLNVLLDIHTKLSGLPVAFRERVCEECNWSTPTFYRKMRGK
                     DKPNPNEKGKIIPALSNAEKQKIIEVMDEVYALGQEYFEKYQRSSK"
     misc_feature    complement(774..787)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.47"
                     /tool="antismash"
     misc_feature    775..788
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.11"
                     /tool="antismash"
     misc_feature    complement(911..925)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 22.77"
                     /tool="antismash"
     gene            1120..1719
                     /locus_tag="HGH90_RS20095"
                     /old_locus_tag="HGH90_20095"
     CDS             1120..1719
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024835.3"
                     /locus_tag="HGH90_RS20095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20095"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_168765130.1"
                     /transl_table=11
                     /translation="MQSSLIHMGHRLKQILKQKKIKIVDFAAMAGFTNQIAHYHLRKND
                     MKRATMERFCTLIDITPDEFMRWNGAMQLSQGKNIHHGDRLQQIIAEKGLNKSKLAGRL
                     DMSRRTMYNLFEKESFSPDELERVAKGLDMTVEGFLNPGTVQESRVTDSEEMMLMREKY
                     YKLLEEHTQLLKSYATIKDELLQVKKEITTLRKQKD"
     gene            complement(1797..2330)
                     /locus_tag="HGH90_RS20100"
                     /old_locus_tag="HGH90_20100"
     CDS             complement(1797..2330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788595.1"
                     /locus_tag="HGH90_RS20100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20100"
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="WP_168765131.1"
                     /transl_table=11
                     /translation="MARIAIDMDNVMADLQQHVLAVYAKEYGEVIDPRRLDNVPEGSVF
                     PEGVMAEMLNTPGFFRNIPVMKGSQEVVKALAAQHEVFIVSAAMEFPFSLAEKLAWLNE
                     HFPFIHWRNIVFCGSKTIVEADYMIDDHDKNLRSFKGTPLLFSAPHNLPLTGYKRLNNW
                     EEVATFFELSEVAA"
     misc_feature    complement(2345..2358)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            2410..3159
                     /locus_tag="HGH90_RS20105"
                     /old_locus_tag="HGH90_20105"
     CDS             2410..3159
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788596.1"
                     /locus_tag="HGH90_RS20105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20105"
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WP_247679922.1"
                     /transl_table=11
                     /translation="MMLKEERLEYILKKLQTDHKVLQAELSSDLQVSEDTVRRDLESLA
                     QNGQLIKVRGGAIPHSPNPYSFKERIQYHEEDKKHIATKALSFLRNGQTIMLDGGTSTL
                     MLAKLIPANLHLTVITNSVPIVSQLMDHPGIELIFTGGLIGKNSQTSGGMDTIRMLEKL
                     RADICFLGICSLHPVAGVTGLDLGEADVKSVMVESANKAIALATSDKMGTAEPFKVCDI
                     TGLDTIITDNPDLPSLKPYINLGIQVI"
     gene            3186..4340
                     /locus_tag="HGH90_RS20110"
                     /old_locus_tag="HGH90_20110"
     CDS             3186..4340
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789819.1"
                     /locus_tag="HGH90_RS20110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20110"
                     /product="MFS transporter"
                     /protein_id="WP_168765132.1"
                     /transl_table=11
                     /translation="MLQDLSMVATNKRATRIAVSALFFLTGLCFSSWASRIPDIQQALG
                     LNDAGLGSLLLALPVGSLISLPVAGLLVSKYGSRQVLLIAGVAYAAVLPALGLAQHAWE
                     LSIFLVLFGFCGNIANIAVNTQAVGVEVIYGRSIMASFHGLWSVAGFTGAAIGAGMSGL
                     HLAPVYHFLIIAGIAWTLIVLTMNKLVTTVPNENNAPSLFGKPDAFLITLGIIAMCSMI
                     CEGTMFDWSGVYFRRVIAVKDGLSGAGYAAFMSTMASGRFIADRLTTRLGVKKMLLISG
                     SLTAAGLLVAVIFPYFYTAMFGFMLVGAGVSAVVPLVYSAAGKSKTLSPGMALATVSTI
                     GYLGFLFGPPLIGFVAQATNLGVAFTMIAVMGAAIAVMSARIKL"
     gene            complement(4343..4996)
                     /locus_tag="HGH90_RS20115"
                     /old_locus_tag="HGH90_20115"
     CDS             complement(4343..4996)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGH90_RS20115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGH90_20115"
                     /product="hypothetical protein"
                     /protein_id="WP_169191777.1"
                     /transl_table=11
                     /translation="MSLLSFQKAITDLIASPQLCLALREQPAEILSRYDLTPREQMRLQ
                     TVVRQPGMSVSCTLYRVNRITPIYTMLPYTCFLLGTSLMPLVEAFWRICYKSDLQFKRE
                     INVFGDFLLEKIAAGALENPYLQEILLMELAINDLKFLPKEALLHTPAEEEALHPLIRL
                     VPFDHSPEPLLTALAGMQLPPALPATGEFLLLVDYRGEELSFSTLPRKSGAVAV"
     gene            complement(5001..5927)
                     /locus_tag="HGH90_RS20120"
                     /old_locus_tag="HGH90_20120"
     CDS             complement(5001..5927)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.3"
                     /locus_tag="HGH90_RS20120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20120"
                     /product="DUF692 family protein"
                     /protein_id="WP_169191778.1"
                     /sec_met_domain="DUF692 (E-value: 6.6e-52, bitscore: 171.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQVISPARTTQLYQQTIPRLGVGLLYNPSLELYLEACPGICDYV
                     EIIPDMFWTDRGNRQQERFEEIESWMIVLDKIAATYPLTAHNISYSLGSAGIFDKTYLQ
                     KMQEWHQRYAFKWHSDHLSFVKVHNDQQEEHNAGLAVPVPYDYEVLDMISGRINAIERA
                     ISTPFLVENNVYFIDLPEQELSETAFLNELSDRTSCGLLLDIHNVYTNAVNHHFDPKEF
                     INQLQLDKVIEIHIAGGNEMAGMYTDSHAGPCPPEVWDLLDYTLPLVHNLCGITFEFHE
                     SYFHLLQFEGITRELNRAKATWNLYHT"
     gene            complement(5980..6219)
                     /locus_tag="HGH90_RS20125"
                     /old_locus_tag="HGH90_20125"
     CDS             complement(5980..6219)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGH90_RS20125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGH90_20125"
                     /product="hypothetical protein"
                     /protein_id="WP_168765135.1"
                     /transl_table=11
                     /translation="MRTAEVHYGANGHIDLTVPKGTKLEDVTKLQALVLRQINPRGCQA
                     CLSGVHFNIREKLEEVINVDLEKMAITKAAVAQH"
     gene            6586..9402
                     /gene="carB"
                     /locus_tag="HGH90_RS20130"
                     /old_locus_tag="HGH90_20130"
     CDS             6586..9402
                     /EC_number="6.3.5.5"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="carB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586614.1"
                     /locus_tag="HGH90_RS20130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_20130"
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="WP_168765136.1"
                     /sec_met_domain="ATP-grasp (E-value: 2.4e-08, bitscore:
                     28.6, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 4.5e-08, bitscore:
                     28.2, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 8.4e-09,
                     bitscore: 30.0, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPKDSSIKSVLIIGSGPIIIGQACEFDYSGSQAARSLREEGIKVI
                     LINSNPATIMTDPMMADKVYLLPLTVESIEQILEENQIDAVLPTMGGQTALNLCKEVDE
                     LGIWEKNNVRLIGVDIKAIDKAEDREQFRQWMIQLGIPVAPARTANSFLEGKEFAQEIG
                     FPLVIRPSFTLGGTGGGFVHSKDDLDEALDRGLKASPIHEVLVEKAVLGWKEYELELLR
                     DKNDNVVIICTVENLDPMGIHTGDSITVAPAMTLSDTAFQEMRNKAMMMMRDLGNFAGG
                     CNVQFSLNPETEELIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYTLDELENQIT
                     KTTSAFFEPALDYVIVKMPRWNFDKFKGADDTLGLQMKSVGEVMSIGRTFPEALQKACQ
                     SLENDALGLGYYGKSLLKSEQLIEKLKRPTWDRIFRIKDALMAGVSVKHIHQLTYIDRW
                     FLHQIQDIVNLEKQLLELDLQSVSVEMLKDAKKMGFSDKQLAILFGDCDEDEVYAKRKE
                     LGITRTFKMVDTCSAEFEAKTPYFYSTFDTENESKVTDKKKIIVLGSGPNRIGQGIEFD
                     YCCTHGLQAIQDCGYEAIMVNCNPETVSTDFDMADKLYFEPVFWENLWEIIELEKPEGV
                     IVQLGGQTALKLAKRLEEKGVKIIGTSFDNMDIAEDRGRFSDMLKELGIPYPKYGTAYN
                     TDDAIEVAKEVGYPVLVRPSYVLGGQRMRIVINEEELEESVLSLLRHLPGNKILIDHFL
                     DRCQEAEIDGIFDGTDFHVMGVMEHIEPAGIHSGDSHALLPAFNLSPIEVTTMEYYAEK
                     IARALNIQGLINIQFAIKDGNVYVIEANPRASRTTPFIAKAYQVPYLNIATKVMIGAKK
                     LKDFTIEKKLTGFAIKEPVFSFNKFPGVNKELGPEMKSTGEAIRFIKDLRDPYFRQLFK
                     EKSMYLSK"
     misc_feature    9405..9422
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: strong, score: 21.55"
                     /tool="antismash"
     misc_feature    9674..9689
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.59"
                     /tool="antismash"
     misc_feature    complement(10102..10120)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.79"
                     /tool="antismash"
     misc_feature    complement(10367..10380)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(10485..10497)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    complement(10731..10747)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
ORIGIN
        1 gagtaacgat aattgcgaaa tattgttttc ctgtaccctt ggttgcaggt agccgatggc
       61 ctggtcgtat tcctggtgct gcaggtattc catcagttga tcgcggtcaa tgggctgctg
      121 gcccagtacc gtttctaaag aacatagcag cagtagcagc gctaggccgc gggttaaaat
      181 ggtgtacata aatatcgttt ttctttttaa aactaagaat ttagttttaa aaagaaaaac
      241 ttagttgata aagataagta aataaaaaag ccttgtaatc ccgggtaggg gtacaaggct
      301 ttattatatg aggatgccat ttaggagtaa gtttctacag acagcgaaag aaatattacc
      361 tcatattatt tagaagatcg ctggtacttt tcgaagtact cttgtcccaa tgcgtacacc
      421 tcgtccatta cttcgatgat tttttgcttt tcagcgttgc taagtgcagg aataatcttg
      481 cctttctcat taggatttgg tttgtcttta ccgcgcattt tacgataaaa agtaggtgta
      541 ctccaattgc attcttcaca cactctttct ctaaaggcca ccgggagtcc ggagagtttt
      601 gtgtgaatgt caagcagaac attcagtgat tctttgctca taatgggtat tattttctaa
      661 ttattggtta tggttaacaa aaagattgac gattatttta agcatataaa acaatatttt
      721 atgtgtaaac aaatatattc ataatcgtgc aatttataat gataaaacat tactttcttt
      781 aaaaaaaata atgccccatg taatatagtg tggtaaacgg tttgaggctg tttgtaaagg
      841 ttagttgatg tgtatcgttt acacaataat tatcagtcat tttattgtga ttatcactac
      901 gatttttatg ttgtcatatt gataaatgtt tgttgttacc atattgcgta tatacgttac
      961 cgttatataa ttgttatggt tgattaatgc tgccgacagg tgtatatttt tttgttaaat
     1021 tgatatattt atttacaaaa tgaaatatta attgttaaat tatacgataa tacaacaggg
     1081 gccatcctgt ctgtagcatc ataatttaaa acataaagga tgcagtcgag cctgatacac
     1141 atgggacacc gcttgaagca gatattgaag caaaagaaga taaagatcgt agactttgct
     1201 gccatggccg gttttaccaa ccagatagcc cattatcatt tacgcaaaaa tgatatgaaa
     1261 cgtgctacca tggagcgttt ctgcacatta atagatatta ctcccgacga gtttatgcgg
     1321 tggaatggcg ctatgcaact tagccagggc aaaaatattc accacggcga ccgcttacag
     1381 cagattattg ctgaaaaagg ccttaataag agtaaactgg ccggccgcct ggatatgagc
     1441 cgtcgtacca tgtacaatct cttcgaaaag gaaagcttct cgcccgatga gctggagcgt
     1501 gtcgccaaag gattggacat gaccgtagaa ggcttcctga atccaggtac ggtacaggag
     1561 tcaagagtta ctgacagtga agaaatgatg ttgatgaggg agaagtatta caaattgctt
     1621 gaagagcata cccagttact gaaaagctat gccaccataa aagatgagtt gttacaggtg
     1681 aaaaaagaaa taaccacact gcgtaaacaa aaagattaaa gaaatacacc ggtcactata
     1741 ataatattca ttataaagcg gggtttcttt aatagctaac ctgtactggg acaccgttag
     1801 gcggctactt cacttaattc aaaaaatgtt gctacttctt cccagttgtt taaacgtttg
     1861 taaccagtca atggtaaatt atgcggagcg ctaaataata agggagtgcc cttgaatgaa
     1921 cgcaggtttt tgtcgtgatc atctatcatg taatccgcct ctacaatagt ttttgagccg
     1981 cagaacacaa tattccgcca gtggataaac gggaagtgct catttagcca ggccagtttc
     2041 tccgctagtg aaaaagggaa ttccatcgcc gcagaaacaa taaatacttc atgttgcgct
     2101 gccagggctt ttaccacttc ctggctacct ttcatcaccg ggatgttcct aaaaaagccg
     2161 ggtgtattca acatttctgc cattactcct tcagggaaca cgctgccttc cggcacatta
     2221 tccaaacgcc tgggatctat tacttccccg tattccttag cgtacacggc taatacatgt
     2281 tgttgcaggt ctgccattac attgtccatg tcaattgcta ttcttgccat aatttgctgt
     2341 tttttgcaaa gttatgcagt atgttgcaga ttattgcata atctttcata attttgcaca
     2401 aaacggcaaa tgatgttaaa ggaagaaagg ctggaatata tcctgaagaa gctgcaaaca
     2461 gatcataaag tactgcaggc agaactcagc agcgatttgc aggtgtcgga agatacggta
     2521 aggagagacc tcgaatctct tgcccaaaac gggcaattga ttaaagtacg gggaggggct
     2581 attccccatt ctcccaaccc ttattcgttc aaggaacgca tacaatatca cgaagaagat
     2641 aaaaaacata ttgccaccaa agccttgtca ttcttgcgta atggacaaac cattatgctt
     2701 gatggaggta cttccacgtt aatgttggcc aaattaattc ccgcaaacct gcacctgacg
     2761 gtcattacca acagcgttcc gattgtttcg cagttgatgg atcatccagg tattgaactg
     2821 atctttaccg gcggattaat tggcaagaac tcgcagacct ccggtggaat ggataccatt
     2881 cgtatgctcg agaaattgcg ggccgacatt tgcttcctcg gtatttgcag tctccatcct
     2941 gttgccggcg taaccggtct ggatcttgga gaggcagatg ttaaaagcgt gatggtggaa
     3001 tcggccaaca aggccattgc actggccacc agcgataaaa tgggtacggc ggagcctttt
     3061 aaagtatgtg atattacagg tttggatact attattaccg ataatcccga cctgccttca
     3121 ttaaagccct atatcaacct gggaatccag gtgatctgat tatttaaatt tttatcttaa
     3181 caaagatgtt acaggatcta tcgatggtgg cgacgaataa gagagcaacg cgtattgccg
     3241 tcagcgcttt atttttcctt actggtttat gtttttccag ctgggcctcc cggataccgg
     3301 atatccagca agccctgggc ttaaatgacg caggactggg tagcctgtta ctggcattgc
     3361 cggtaggatc cctgatttcc ctcccggtgg cgggactact ggtatcaaaa tacggcagcc
     3421 ggcaggtatt attgattgcc ggtgtagcct atgccgccgt attacctgcc cttggcctgg
     3481 cccagcatgc atgggagctg agtattttcc tggtattgtt cggcttctgc ggcaacattg
     3541 ccaatatagc ggtaaacaca caggcagtag gtgtagaggt aatatatgga cggtctatta
     3601 tggcttcctt ccacggcttg tggagcgtag cggggtttac aggcgccgcc ataggagccg
     3661 gcatgtctgg gctacacctg gcaccggtat atcacttctt aatcattgcc ggtatcgcct
     3721 ggacgctgat tgtattaacc atgaataagc tggtaactac cgtgcccaac gaaaataatg
     3781 caccttctct atttggaaaa cctgatgctt tcctgattac gttgggtatc attgccatgt
     3841 gttctatgat ttgtgaggga acaatgttcg actggagcgg tgtatatttc cgtagagtga
     3901 tagccgtgaa ggatggttta tccggtgcag gctatgctgc gtttatgagc actatggcgt
     3961 ccggacgatt tattgccgat cggcttacca ccaggctggg tgtaaagaag atgctgctga
     4021 tcagcggaag ccttaccgca gcagggttac tggtagccgt tattttccct tatttctaca
     4081 cagcaatgtt tggctttatg ttggtaggcg ccggcgtatc agccgtagta ccgctggtat
     4141 acagtgctgc cggcaaatca aaaacactat ctcccggtat ggcgttggct accgtatcca
     4201 ctatcggtta cctgggattc ctctttggcc cgccactgat tggatttgtg gcccaagcta
     4261 ctaacctggg agttgccttc accatgattg ctgtaatggg agcagctatc gccgtaatgt
     4321 ctgccaggat aaaattataa acttacactg ccactgcgcc gcttttgcgg ggcagtgtgc
     4381 tgaaggagag ttcctctccg cggtaatcta ctaacagtaa aaattccccg gtggcaggaa
     4441 gagccggcgg cagttgcatg ccggcaaggg ctgtcagtaa aggctccggt gaatgatcaa
     4501 aaggaaccag tcttattaat gggtgtagtg cttcttcttc cgcaggtgta tgcagtaaag
     4561 cttctttagg caaaaatttc aggtcattaa tggctagttc cattaacaaa atctcctgca
     4621 aatatgggtt ttcaagggcc cccgcagcta tcttctcaag taaaaaatca ccaaatacat
     4681 tgatctcccg cttaaactgc aaatcggatt tatagcaaat acgccagaat gcttctacaa
     4741 gcggcatcaa tgatgtgcct aataaaaaac aggtataggg aagcatcgta tagatagggg
     4801 tgatcctgtt tacacgatat aaggtacaac tcactgacat gcccggttgg cgtactaccg
     4861 tttgcaaacg catttgctcg cggggggtaa gatcataccg ggagagaatt tccgccggct
     4921 gctcccgtaa cgccaggcac aattgcgggg aggcaattag atctgtgatc gctttctgga
     4981 aagagagtaa tgacatatca tcaggtatga taaaggttcc aggtagcttt cgcccggttt
     5041 agttcgcggg taataccctc aaactgcaac aggtgaaagt aagattcatg aaattcaaag
     5101 gtaatgccgc ataggttatg cactaacggt aatgtataat cgagcaagtc ccatacctcc
     5161 ggtggacaag ggcctgcatg agaatcagtg tacatgccag ccatctcgtt gccgccggct
     5221 atatggattt cgatcacttt atccaactgt agctggttga taaattcctt gggatcaaaa
     5281 tgatgattaa cggcattagt atatacgttg tgaatatcga gtagcagccc acaggaggta
     5341 cgatctgata actcattcag gaaagccgtt tcgctgagtt cctgctccgg caaatcgata
     5401 aagtatacat tgttctctac caggaaaggg gtactgatag ccctttcgat ggcattgata
     5461 cggccgctga tcatatccag cacttcatag tcgtacggca ccggtactgc gagtcctgca
     5521 ttatgttctt cctgttgatc gttatgtact tttacaaaag acaggtgatc actatgccat
     5581 ttaaatgcgt aacgctggtg ccattcctgc attttctgaa ggtatgtttt atcaaaaatg
     5641 ccggcgctgc caagcgagta agatatgtta tgagcggtta atgggtaggt agcggctatc
     5701 ttatccagca caatcatcca ggattcaatc tcttcaaacc gttcctgctg gcggtttcca
     5761 cgatctgtcc agaacatgtc gggaatgatt tccacataat cgcaaatccc cggacaagct
     5821 tccaggtata gctccaggga ggggttgtat agcagcccta cgcctagccg gggaatagtt
     5881 tgttggtaca gctgggttgt acgggcaggt gatattactt gtaacatgac aggaacattt
     5941 aaataccgtc ctggcacagg ccaggacggt atcaccggat taatgttgcg caacggctgc
     6001 cttggtaata gccatttttt ccaggtctac gttaatcact tcttccagtt tttcccggat
     6061 attgaagtgt acgccagaca ggcaggcctg gcaaccgcgg gggttaattt gtctcagcac
     6121 caatgcttgt aatttggtga cgtcttccag tttagtgcct ttgggaacgg tgaggtcgat
     6181 atgaccattt gcgccgtaat gaacttcagc tgtacgcatg acgtttgatt tttggggtgt
     6241 aaaatgtatg tgtaaaaaac gaaaacgatt ctttggggta gaagctcttg tattcgttgc
     6301 gccagtcttt tgagaatgct gtaccataaa gttaggagaa taaatgaata tctcgataag
     6361 tatttatacg gaaagtggat gccggtcaaa actacgttag gccgaccgga gtagatatta
     6421 gtaattaaat ctatattagt tattagacta tcgtcaaaaa aaacttccat ttttttaaat
     6481 tttttgcgct aaaaagattc taatcacgta actttgcacg atttttttgg tccctcacac
     6541 ggcataaaga tcaatttggt ttttcatttt caatagatta cacaaatgcc aaaagactca
     6601 tctatcaaat ctgttctcat tatcggttct ggacccatta ttattggtca ggcttgcgaa
     6661 tttgattatt ccggatctca ggcagcgcgt tcgctgcggg aagaaggaat taaagtgata
     6721 ttgattaatt ccaacccggc taccatcatg acggatccaa tgatggccga taaggtttat
     6781 ttgctgccat tgaccgtgga aagcattgag cagatcctgg aagaaaacca gatagatgcc
     6841 gtattaccca ccatgggcgg ccaaactgcg ctgaaccttt gtaaagaagt agacgaactg
     6901 ggcatctggg aaaagaacaa tgttcgcctg atcggggtag acattaaagc gattgataaa
     6961 gcagaagatc gtgaacaatt ccgtcagtgg atgatccaac tgggtatccc ggtggcgccc
     7021 gccagaacag ctaactcttt cctcgaagga aaagaatttg cacaggaaat aggtttcccg
     7081 ctggtaatcc gtccttcctt taccctaggt ggtaccggtg gtggatttgt acacagcaaa
     7141 gacgacctgg atgaagcgct ggaccgcggt ttgaaagctt ctcctatcca tgaagtgctg
     7201 gtagaaaaag cggtactcgg atggaaagaa tatgaactgg aactgttgcg cgacaagaat
     7261 gataacgtag taatcatttg tactgtggag aacctcgacc cgatgggtat ccacacaggt
     7321 gattccatta cggtggcgcc agcaatgacc ctgagcgata cggctttcca ggagatgcgt
     7381 aacaaagcga tgatgatgat gcgcgacctg ggcaattttg ccggtggttg taacgtacag
     7441 ttttcgctga acccggaaac agaagagctg attgctatcg aaatcaaccc tcgtgtgagc
     7501 cgttcctctg cattggcctc taaagcaacg ggttacccga ttgcgaaaat tgctgccaaa
     7561 ctggctattg gttatacact ggatgaactg gaaaaccaga tcaccaaaac tacttccgca
     7621 ttctttgaac cagcactgga ctacgtgatc gtaaaaatgc cgcgctggaa cttcgataaa
     7681 tttaaaggag ccgatgatac cctgggcctg caaatgaaat cagtgggaga agtaatgtct
     7741 atcggacgta ctttccctga agcattacag aaagcctgtc agagtctcga aaatgatgca
     7801 ctcggcctcg gctactatgg taagtctctc ctgaagagtg agcaactgat cgagaaatta
     7861 aaacgtccta cctgggatcg tattttccgc atcaaagatg ctttgatggc aggagtttcc
     7921 gtaaagcata ttcaccagct cacttatatc gaccgctggt ttctgcacca gatccaggac
     7981 atcgtaaacc tggaaaaaca attgctggag cttgatctgc agagcgtttc agtagaaatg
     8041 ctgaaagatg ccaagaaaat gggcttctct gacaagcaac tggcgattct gtttggtgat
     8101 tgcgatgaag acgaagtata cgctaagcgt aaggaactgg gcattacccg gactttcaaa
     8161 atggtagata cctgtagcgc ggagtttgaa gcaaaaacac cttacttcta ctctacgttc
     8221 gataccgaaa atgaaagcaa ggtaaccgat aaaaagaaga tcattgtatt ggggtccggt
     8281 cctaacagaa tcgggcaggg tattgagttc gactactgct gtacacacgg tttacaggcg
     8341 atccaggatt gcggttatga agctatcatg gtgaattgta atcctgaaac ggtttccacc
     8401 gacttcgaca tggccgataa actatacttc gagccggtat tctgggaaaa tctctgggag
     8461 atcattgagt tggaaaaacc ggaaggcgtg attgtgcagt tgggtggaca aaccgcgttg
     8521 aagctggcca aacggctgga agagaaaggg gtgaaaatca tcggtacctc tttcgacaac
     8581 atggatattg cagaagaccg cggtcgtttc tccgatatgc tgaaagaact gggtattcct
     8641 tatcctaaat atggcactgc ttacaataca gacgacgcga tagaagtggc gaaagaagta
     8701 ggttacccgg tattggtgcg tccttcctat gtattagggg ggcagcgtat gagaatcgtt
     8761 atcaatgaag aggagctgga agaatctgta ctgagcctgt taagacactt gcctggtaac
     8821 aagatattga tcgatcactt cctggatcgc tgccaggaag cagaaataga tggcatcttt
     8881 gatggtactg atttccatgt aatgggtgtg atggagcata ttgagccggc gggtatccac
     8941 agtggcgata gtcatgcgtt gttaccagct ttcaacttgt cgccgatcga agtaactacc
     9001 atggaatact atgcagaaaa gattgcgcgt gcactgaata tccagggctt aattaatatc
     9061 cagtttgcaa ttaaagatgg caatgtatat gtgattgaag cgaatccgcg tgcttcccgt
     9121 actacaccgt ttattgctaa agcctaccag gtgccttacc tgaacattgc taccaaagtg
     9181 atgattggcg ccaaaaagct gaaagacttt accattgaaa agaaacttac cggttttgct
     9241 atcaaggagc ctgtattctc tttcaacaag ttcccggggg taaacaagga actcggacct
     9301 gaaatgaaat caaccggaga agcaatacgc tttatcaaag atctgcgcga tccatatttc
     9361 agacaactct tcaaagaaaa gagtatgtac ctgagtaaat aatattgtta gctaagtgtt
     9421 aacgaaaagc cctcctcgct atagcgggga gggcttttta tataaagtaa ggttgtcaaa
     9481 aaaacagagc ctgataaaaa tcaggctcct tcttttccct caaaataacc attaaagaca
     9541 cgactattga atcttgatac aattcatata gcgtgaccgg taatgcagtt tacaaataat
     9601 attatattta aaagagatgt taactaatca ataacagatg aaataatata taatatttta
     9661 ataattgatt attaattgtc tagacaagag acatttgtta ccgatttcag ccatttttct
     9721 cattataagg agaagttttc aagacatcat ataaagatat tatctattaa ctaaacccat
     9781 ttttaacatt tttgaaacac tttacaggat gttaaaagtt aggtttgttt ttgataaaag
     9841 ttaaccccac atgcattttg agtctacagc atttactgtg atgtgctgga aacaagcacc
     9901 attcttacgc cttattttac ccttcctggc ggggataagt atacaatttt atacagcgtt
     9961 gccgccactt ttgtggctat gtacagtggt tgtttgtacc tgttgtgcat ggcagtttcg
    10021 ctatctgcct gtacaatggc ggtttaaaca ggcgtggttg ccaggtatgc tgttacagat
    10081 aagcgtggcc ggaagtgggg ccctggtttt atataaagca gatacccggc atcaacaggg
    10141 ctttttccag gataacagaa cggatacatc cctggtttta ttgcagatag aagagccgtt
    10201 gcaaccgaaa gcaagatcat ttaaatccat tgccagggtg atagcgctat ttatacaaca
    10261 ccggtgggtg cccgcaaaag gcaatttgct gctttatatt gcccgggata ctatgcctcc
    10321 gctgcttacg tatggcaacc ggctcgttgt ccgtaaaaag ccagaattga tcaggaacaa
    10381 cggcaatccc ggaggcttca actatctgca atattgtgaa tctcaacagc tttatcagca
    10441 gctgtattta caggctaccg attattacaa attatccggc acgggtggca gtgtcataaa
    10501 tcgttggttg ttgcatgcga gagattactg tttatcctgt tttaggagat atattggggc
    10561 agggccggaa gccggaatgg cagaagcatt gctgataggg taccgacagg atctggataa
    10621 ggcagtggtg caagactata gtaacactgg tattgtgcac attatcgcta tttccgggat
    10681 gcacctggcg ctgttgtatg gtacgctcct ctggtgtttg cgctggttgc cgcccggcaa
    10741 atgggcaggt gcattgaaag cgattcttat tttgggagtg ctatggggat ttgcactgct
    10801 taccggggca tcggcttctg tgctccgttc cgcggtgatg tttaccggca ttacgcttgg
    10861 ccgttttata ctggagcgtt acagcagcat ttataatacc ctggctgcat ccgcgttttt
    10921 gttatta
//
