LOCUS       NZ_JABAOB010000001     20996 bp    DNA     linear   CON 23-OCT-2023
DEFINITION  Chitinophaga sp. Ak27 Ak27-scaffold_1_length_1986630_cov_140.309,
            whole genome shotgun sequence.
ACCESSION   NZ_JABAOB010000001
VERSION     NZ_JABAOB010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga sp. Ak27
  ORGANISM  Chitinophaga sp. Ak27
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1  (bases 1 to 1986630)
  AUTHORS   Yin,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2020) State Key Laboratory of Plant Genomics,
            Institute of Microbiology, Chinese Academy of Sciences, Beichen west
            road, Bejing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-AUG-2019
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 246.8x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/23/2023 02:22:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,312
            CDSs (total)                      :: 6,224
            Genes (coding)                    :: 6,211
            CDSs (with protein)               :: 6,211
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:12:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 514774
            Orig. end                         :: 535770
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABAOB010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20996
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20996
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20996
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10996](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10996)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(44..64)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.85"
                     /tool="antismash"
     misc_feature    complement(493..513)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.53"
                     /tool="antismash"
     gene            complement(1015..1779)
                     /locus_tag="HGH90_RS02095"
                     /old_locus_tag="HGH90_02090"
     CDS             complement(1015..1779)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216350.1"
                     /locus_tag="HGH90_RS02095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02090"
                     /product="response regulator"
                     /protein_id="WP_168763355.1"
                     /transl_table=11
                     /translation="MTESILIVDDNEDILEFLSVVLGDSYQLHLAANGEIAQTVLDKEI
                     IHLIISDIMMPGIDGFELCRLIKSNVEYCHIPIILLTSKNTYKAHIEGLEVGADAYIQK
                     PFSSELLQVQIANLLKNRRKIKEHFASSPFEDVRVMAHSKTDEAFLKKLDEYIRENIKD
                     PNIDIDMLAGHMFMSRTTLYRKIKSLSSLSPKELIDITRLKKAANLIAQNEFSFYEISK
                     MIGYSSQSLFSRNFQKYFKMSPLEYFDSLPRQ"
     gene            complement(1781..4972)
                     /locus_tag="HGH90_RS02100"
                     /old_locus_tag="HGH90_02095"
     CDS             complement(1781..4972)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216351.1"
                     /locus_tag="HGH90_RS02100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02095"
                     /product="sensor histidine kinase"
                     /protein_id="WP_248276956.1"
                     /transl_table=11
                     /translation="MMRSFGHIVFLLNLLLLVAISPCYSQYYFKHYQVDDGLVHNAVTA
                     VIQDSTGLIWVGTRGGLNRFDGYTFKAYKNNNDKFGSLGNDVINCIAEDKNRMIWIATG
                     KGIFKYNPYKEVLTELETVPKEYTNHLVVDNDNNLWFLIQFSLYKYIQAENRVENLKIK
                     ASCIALDSNKNLWLGDNDGNIHIYNPKAKPGDRIRIVDRSLPVNARSISKIYPIGNNEL
                     LIGCFKQGLKSYNTTTGLVKSLSLDGDKHKDIFVRDIAVGNDGQYWIATESGIYIYDLA
                     INKSISLKKRPGDPYALADNAVYAICRDNQGGMWAGTFFGGLNYYSKVNARFEKYYPLP
                     GTNAISGNAISEICADKNGHLWIGTEDAGISKLDVKTGLFTNYTATGKKGSISYSNIHG
                     LFSWGNALFIGPFLQGMEIMDVRTGLVTNRFKLIGDKTGPMSDFVISIYRTKNNKLLVG
                     TAYRNAGLFEYDIQHKTFRRISQIPFNTYVYDIFEDSAGNIWTGSVKEGAFYYNPETGQ
                     RGNLRFGDTTRGKTINEFPVYNIFEDSDHALWFATTGSGLIKLNPDRKTTKRYTTANGL
                     PSNVLYSILEDNSKHLWVSSSKGLVCFDLHTEQVKVYTQANGLITDQFNYNSAYKHTDG
                     KMYFGSVKGMIAFDPALFSQREPSPPTYITGFQINNIEAVPGGHNSPLPKSILYTDTIV
                     LKYDQNNFSIEFAALNFSSPGATRYKYLMKGIDQPWTYLNTNRKAYFTDLSYGSYEFIV
                     QAESNTGSWVGRERRLFIKILPPFWKSNVAYILYLLLLGTVIFITDRLYRRYLQRKNLR
                     KLQLFEHEKEKEIYQAKIEFFTNITHEIQTPLTLIAGPIEWLSKKFDKEPHVKKSLKIA
                     EKNTRRLIELASQLLDFRKTEADQFSLNFVKTDIVRLITELVAGFKEQAAANHINLNIV
                     LPGKPFMAFVDREAFNKICTNIVSNAVKYAATRATISINTVNDTDEYFTISFSNDGKGI
                     PAEFKDQIFEPFVRLRGNDRPGTGIGLSLAKSLTELHNGSLRLISGETDLIIFELKLPI
                     HQKFEFQLSSWKKIK"
     misc_feature    complement(5180..5198)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.15"
                     /tool="antismash"
     gene            5252..5512
                     /locus_tag="HGH90_RS02105"
                     /old_locus_tag="HGH90_02100"
     CDS             5252..5512
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGH90_RS02105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGH90_02100"
                     /product="hypothetical protein"
                     /protein_id="WP_168763356.1"
                     /transl_table=11
                     /translation="MNKKFTFSQKPLDYVYALVWFGLFVAFAYYIFSGKMFQPDANGKI
                     HIVAILVAWVAEYLTQIGTSILVLLIGLYISGKMIVKKAVN"
     gene            5520..5723
                     /locus_tag="HGH90_RS02110"
                     /old_locus_tag="HGH90_02105"
     CDS             5520..5723
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGH90_RS02110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGH90_02105"
                     /product="hypothetical protein"
                     /protein_id="WP_168763357.1"
                     /transl_table=11
                     /translation="MQVNFIILLFTGIYLAGTLLYYRYAVKKGIAFRYKPITLIVVFLL
                     FLLALYGIITRQPYNEILPFIR"
     gene            complement(5750..6700)
                     /locus_tag="HGH90_RS02115"
                     /old_locus_tag="HGH90_02110"
     CDS             complement(5750..6700)
                     /EC_number="3.1.4.17"
                     /GO_function="GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218757.1"
                     /locus_tag="HGH90_RS02115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02110"
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="WP_169190272.1"
                     /transl_table=11
                     /translation="MKPVLTILLFACVLFCKTAFSQTSFKVIPLGVKGGIDESNLSAYM
                     IATTGSNDYVCLDAGTLHYGIEKAIQAKLLHGTAEEVLKKNIKGYLISHAHLDHLAGMI
                     INSPDDSSKNIYGLDYCLDVLKDKYFSWKSWANFADEGEKPALGKYHYTVLTPGIEMPL
                     QNTDMQVTAFSLSHGNPYQSTAFLVRHNEAYALYLGDTGADSVEHSDKMHQLWQYVAPL
                     LKAKKLKALFIEVSFANEQPDKQLFGHLTPRWLMSSLENLASLSGKEALNNFPVVITHI
                     KPGGNREQVIRTQLKAYNPLHVKLIIPEQGKLLTF"
     gene            6756..8336
                     /locus_tag="HGH90_RS02120"
                     /old_locus_tag="HGH90_02115"
     CDS             6756..8336
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024629.3"
                     /locus_tag="HGH90_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02115"
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="WP_169190273.1"
                     /transl_table=11
                     /translation="MKPVHLLYLLALVKLILPFFLQDPTWGPHRDEFLYLAEARHMAWG
                     YMEVPPLLSVFAWLTNLLGGATWAIKIWPSLGGALTYVLAGRLVLHLGGRWFALVLAWM
                     PFVAGVWLRIHFLFQPNFLDILFWTAMAYGLTRFQQSEKPIHLYMAGLSFGLGMLSKYT
                     MVFYATGLMAGLLFTWDRKILTNRHFYYAIGLGFLLFLPNLIWQVRNGFPVVYHMKALH
                     DGMLQHVSNSDFLKDQLLYNVATLFTWVTGLIWAARTKQYRFIAWAFFVTLTLLMVGHG
                     KSYYSQGAYPILFALGAPQLEAWANKWWKIGMIAFSLFIGIRLTPLLLPFKAPTPLAAY
                     YVRHPLASKVGFLRWEDLQDHSLPQDFADMLSWEEMTQKVANAYASLNNTEKAHTLLFC
                     DNYGEAGAVNYYGPKYHLPAAYSDNASFLYWMPRDFDQFEVLLLVTDDKQEMQHAFIKE
                     FKAVQLVDSITNPYAREHGSLIILMKGPSEAFRKAFRDKIDKSKLKTTADGAAQTLKPN
                     PLEDGGALH"
     gene            complement(8337..9245)
                     /locus_tag="HGH90_RS02125"
                     /old_locus_tag="HGH90_02120"
     CDS             complement(8337..9245)
                     /EC_number="3.8.1.5"
                     /GO_function="GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (38-287). E-value:
                     2.5e-21. Score: 68.3. Matches aSDomain:
                     nrpspksdomains_HGH90_RS02125_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584448.1"
                     /locus_tag="HGH90_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02120"
                     /product="haloalkane dehalogenase"
                     /protein_id="WP_169190274.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-14, bitscore: 50.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEHKANVQTIFSEKPFAEKKFIEIKGRRMAYIDEGEGDPIVFQHG
                     NPTSSYLWRNIMPYCRGLGRLIACDLIGMGDSEKLPGSGPEQYAYSVQRNFLFALWEEL
                     KLERNVTLVLHDWGSVLGFDWANQHRSRVQGIVHMEALAIPFKWDEFEPKVRDLFRALR
                     SPAGEELVLKNNVFIEKVLPGGILRELSEAEMAEYRRPYLNEGEDRRPTLSFPRQIALD
                     GSPKEVADVVTDYGNWLKESPVPKLWIHGNPGAVENDSLREFCSTWPNQAEITVKGKHF
                     LQEDSPGEIGEAIAGFVKKLR"
     aSDomain        complement(8385..9131)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HGH90_RS02125_Abhydrolase_1.1"
                     /evalue="2.50E-21"
                     /label="HGH90_RS02125_Abhydrolase_1.1"
                     /locus_tag="HGH90_RS02125"
                     /protein_end="287"
                     /protein_start="38"
                     /score="68.3"
                     /tool="antismash"
                     /translation="PIVFQHGNPTSSYLWRNIMPYCRGLGRLIACDLIGMGDSEKLPGS
                     GPEQYAYSVQRNFLFALWEELKLERNVTLVLHDWGSVLGFDWANQHRSRVQGIVHMEAL
                     AIPFKWDEFEPKVRDLFRALRSPAGEELVLKNNVFIEKVLPGGILRELSEAEMAEYRRP
                     YLNEGEDRRPTLSFPRQIALDGSPKEVADVVTDYGNWLKESPVPKLWIHGNPGAVENDS
                     LREFCSTWPNQAEITVKGKHFLQEDSP"
     misc_feature    complement(9290..9299)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(9307..9894)
                     /locus_tag="HGH90_RS02130"
                     /old_locus_tag="HGH90_02125"
     CDS             complement(9307..9894)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584452.1"
                     /locus_tag="HGH90_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02125"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_169190275.1"
                     /transl_table=11
                     /translation="MNDTIIKNLRRHIDPTPEETAIFLSLAVAKTVKRKEILLKEGDVA
                     RQQFFVINGCLRTYTIDASGKEHVLMFAPEDWWCSGDLYSFLSGQKSINNLEALEPTTL
                     LQISKDNLDILFRQAPTFERFFRILFQNAFVFHQNRINANLSQSAEGRYELFTKAYPDL
                     ESRISQKYIASYIGVTPEFFSQMKANMFRKKS"
     misc_feature    complement(9913..9926)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            complement(10001..10996)
                     /locus_tag="HGH90_RS02135"
                     /old_locus_tag="HGH90_02130"
     CDS             complement(10001..10996)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.2"
                     /locus_tag="HGH90_RS02135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02130"
                     /product="terpene synthase"
                     /protein_id="WP_169190276.1"
                     /sec_met_domain="T1TS (E-value: 4.9e-41, bitscore: 138.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAKMLSLFSYPFPTLKNPFADALQEITDNQWIDGEYLWLYKDDP
                     QTRQKYKKTKTAHIASQWFPTSSQERLKPVCRFMLWTLFNDDKYEEGTPEQIRFIHQQS
                     IAVLKGEVNAAQSGIPLGGLLASLREELLQFISEESLRRFITGLSRYFRGLELELEYKQ
                     KRTFPSIEECIAIRENSLCLYPFLELTDLETGIPLPPGVNEHPVLQRLRALAVRMMVYF
                     NEVQSVVKDEATGSIYYNVVKAIQYNKNLSLEEACLEDLRIHNEELKEFVRLQQSLPDF
                     GEWQDVVVNRVHYISMTLSGWKSVSSRLERYNSLQGFPSAQAIKKTVLPD"
     misc_feature    11090..11104
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.98"
                     /tool="antismash"
     misc_feature    complement(11091..11106)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.39"
                     /tool="antismash"
     gene            complement(11167..11709)
                     /locus_tag="HGH90_RS02140"
                     /old_locus_tag="HGH90_02135"
     CDS             complement(11167..11709)
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222387.1"
                     /locus_tag="HGH90_RS02140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02135"
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="WP_169190277.1"
                     /transl_table=11
                     /translation="MDRRELIKTIAVLTGAAFVGGELFLSGCKEQKGPLFSSDDIAFLD
                     EVAEAIIPRTDTPGAKDAEVGKFMALYAAGCYDNTQQQLLKQGITQLNTASKEKFKQAF
                     VQLPTKQKQSLLTGIDEAAKRQHNNDDAPHYFTLMKQLTLLGFFTSKAGATQVLRYLPV
                     PEKYEGCIPYNGETAWS"
     gene            complement(11710..13410)
                     /locus_tag="HGH90_RS02145"
                     /old_locus_tag="HGH90_02140"
     CDS             complement(11710..13410)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587088.1"
                     /locus_tag="HGH90_RS02145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02140"
                     /product="GMC family oxidoreductase"
                     /protein_id="WP_169190278.1"
                     /transl_table=11
                     /translation="MADNSDAVKQFDAIVIGSGISGGWAAKELCEKGMKTLVLERGRNV
                     EHVKDYPTANTPSWEFKYRGAVSKEVLKANPYISRAAGYDDSTAHFFVKDADHPYIQEK
                     PFEWIRGYQVGGKSLIWGRACARWSEMDFTAPHRYGYGIEWPIGYDDIAPWYSHVEKFI
                     GVCGNKEGIATMPDGEFQPAFELNCVEQHLSDVVREKFNRHYVSGRWAQVTQPQEVQKE
                     QHRQCLSRNLCMRGCPFGGYFSSNASTLPWAEKTGNLTIRPFSVVHSIIYDEQSGKATG
                     VKVIDAKTKEAMVFHAKIIFLNASALNTNLVLLNSTSKRFPNGLGNDNGLLGKYICFHN
                     YRAGMSGEFDGFEDKYYKVSKPSECIIPNFRNVYEKDTDFVGGYVIFSAAYREKLSDKN
                     LPAAVGAKFKEAISKPGKWGAYMYMQGETIPKESNHVRLSKDKKDEWGIPLLITSVDYD
                     DNDDRMVKDFIEQGKAMLTAAGAKNIAVYDNHQPPGLDIHEMGGVRMGKDPATSLLNEW
                     NQLHHCKNVFVTDGACMASTGNQSPSILYMSLTARAATYAVDQLKKGAF"
     gene            complement(13422..14300)
                     /locus_tag="HGH90_RS02150"
                     /old_locus_tag="HGH90_02145"
     CDS             complement(13422..14300)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012692.3"
                     /locus_tag="HGH90_RS02150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02145"
                     /product="ROK family protein"
                     /protein_id="WP_169190279.1"
                     /transl_table=11
                     /translation="MEKTQIIGVDLGGTNLRAGIVNEGICESVVAQPLYAQGSRAQILE
                     QIFGVIDKVISPGVKAIGIGVPGLVDPGSRIPYDIVNIPSLNETDLRTVLEQRYQVAVK
                     IENDANCFALGEFHFGAGRSCTSLVGLTIGTGLGAGIIVDGKLFTGKHGGAGEFGMIPY
                     GGKNIEYYVSGRFFSNVYQIAGEEAYRRALEGDPEAIKMYGDFGRHLGEAIKLIMYVLD
                     TECIIIGGAVKAAFPLYAKSMWATLGDFEFSRSLAALKIETSSLINGNILGAAALYLSA
                     ADGRPYSMNNQ"
     gene            complement(14302..15036)
                     /locus_tag="HGH90_RS02155"
                     /old_locus_tag="HGH90_02150"
     CDS             complement(14302..15036)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013356.3"
                     /locus_tag="HGH90_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02150"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_169190280.1"
                     /transl_table=11
                     /translation="MKIAIYQTYAELSAHVAEQVIQLMSAHEKPLLCPASGDTPAGLYK
                     ALAEKREQQQYEATDWSFVGLDEWVGLNGTDEGSCRYSIDKELFRPLDVSENNIYFFDG
                     RSADLRGECSAAEQFIRERNGIDVAVLGVGMNGHIAMNEPGCAIDGRTQVITLHPVTKQ
                     VGQKYFSQPQVLDKGITLGMGTLSESRHIILMVSGQHKAEIVRKMLEESVTNELPASLL
                     LQHPSVTVFLDAAAAASLSNKQ"
     gene            complement(15045..16142)
                     /locus_tag="HGH90_RS02160"
                     /old_locus_tag="HGH90_02155"
     CDS             complement(15045..16142)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014893.3"
                     /locus_tag="HGH90_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02155"
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_169190281.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 1.2e-31, bitscore:
                     106.5, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 5e-29, bitscore:
                     97.4, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVKVALLGAGFIADIHMESYTRFVPEAEVVAVYTRNAEKAKAF
                     ADRHHIAQHFSSIDELLQQVEVDVVDICLPNFLHKEATLKAAAAGKHIIIEKPLAVTLE
                     EADEMIAACEAANVKLMYAEELCFAPKYERARKLVEEGAVGEIYMLKQSEKHSGPHSDW
                     FYDVSQSGGGVLMDMGCHGIAWFRWMLKNSKAVSVTASMKTVFHKNRTRGEDNSVVIIE
                     FENGVTGVVENSWAKQGGMDDRSEIHGLDGVAYADLFTGNSVLAYSKNGYGYAMEKTDT
                     TIGWSFCIFEEAFNQGYPQELKHFIECVQHDREPLVTGKDGRAVLEIIYAAYASAGQGK
                     RITLPFNQQVAKPIDLWLHPTSTTK"
     gene            complement(16492..17304)
                     /locus_tag="HGH90_RS02165"
                     /old_locus_tag="HGH90_02160"
     CDS             complement(16492..17304)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012925.3"
                     /locus_tag="HGH90_RS02165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02160"
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="WP_169190282.1"
                     /transl_table=11
                     /translation="MKRSIYCLVLAWVGCCFIATSQAQSIKRHTKWKLTWSDEFNYKGL
                     PDSTKWGYQTGRSGWGNNELQFYTAADTNNAKVEKGKLSITARNGGSGDHQYTSARMIT
                     KNKGDWKYGKLEVSAKLPKGRGLWPAIWMLSTGDEYGGWPESGEIDVMEHVGYMKDSIF
                     GSLHSKTYNHVIGTQKTKGVYIKQPYDKFHVYAIEWTPDDITFLLDGKVYYQVANEHKG
                     HKGWPFDKKFYLLLNVAVGGNWGGKEGVDEAVFPAAMQVDYVRMYEAI"
     misc_feature    complement(17290..17305)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.45"
                     /tool="antismash"
     gene            complement(17337..18872)
                     /locus_tag="HGH90_RS02170"
                     /old_locus_tag="HGH90_02165"
     CDS             complement(17337..18872)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019592.3"
                     /locus_tag="HGH90_RS02170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGH90_02165"
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_168763369.1"
                     /transl_table=11
                     /translation="MRNYISILLIVLLLSSGCSKFLDRPLENKAQATNIDYTDLSLMYQ
                     PVSGVYRTAASGTFGKWISMAIRSSRSDDIEPGNDDAGQIGIHNFQTDVTVKSYWGIND
                     MWISMYAVVIAANNALYELDQFGKHIPASDADKTKLLARYQAEVRFYRALAQFWLGRSF
                     GAVPILGVETIDPAKLGSVGKSSLEDVNKYVMAEMDFCIPNLEDARPNAAAHVGGVTKY
                     TALMLKAKAAMDLAGNNNGSTYWDVVLDCTNQIVNSGKFSLFSNYYQLFKMPGKLCDES
                     ILELQYSDFGKSTGDIVISGGPGEEWGNFFFFQGPENTYSSVITGPGWMVPTQKAVDFL
                     KSRNDSVRLKMAIQYCGVNGAPGTYSVTPDGDTISGNGSRKKYFNGKSYTPRSQMTPGR
                     VDYYGANNNVRIMRYAEALLMNAEAKIRKGQSGDAQVNLVRNRVKLASINGVTLQQLLD
                     ERHAELICEWWGERFNDLLRTDQAATVLPGFIKGKSEYIPIPQAQEDINSLLK"
     misc_feature    complement(20646..20659)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.54"
                     /tool="antismash"
ORIGIN
        1 ggacctatgt ggaatatttc caccctggtt tctccggcag ggaaatttat gcaatgctac
       61 gcttcatcca gcaactccta ccgggccaac ctggattaca actcggcggt gggccgcggt
      121 atcgccatga tcagtcctac ctattttgcg cagcatgaac tttggaacgt aaatggcatt
      181 aatgataccg cagatttgcg gcataacagc cgggtgggca attgggcaag aatggactct
      241 ctgaaatata atgatccttc agatgcctta tactttggta aaaacctgag actctataat
      301 ggaacaaaac ttttgtgtac cgatacggtg cgtaattggt tcgactggcc acactataaa
      361 gtgtttgcaa atgatattga tcatattggc aatccggcga gtaataataa taggggcggg
      421 gcggccgact ggtactgtta tcgtttggcg gaaacctacc tgcttcgtgc ggaggcgcaa
      481 ttttacaaag ggaatatagc cgcagctaca gcagatgtaa atattgtaag gcaaagagca
      541 aaatgccagc aattatacac cgccgttaca attggcgaca tcgccaacga acgtgccagg
      601 gaactctgga tggaagaatg gcggcacatg gagctaagcc gtatgtccta cagcctcgcc
      661 ttaagcggca aagccgatga atggggcaat acatataacg tgaacaccct gtcagacaac
      721 agctattggt atcagcgcat acagcattac aacaattact acaacaaaaa caaaatcacc
      781 ataaaaggaa gatcctatac catggcgcca cataatatct attggccaat tccccaatca
      841 gctatcgatg ccaacttata tggcaagctg cgtcaaaatc ccggctataa cggctatgcc
      901 ccggatgtag agatgtggac gaaatgggaa aacgcggtga atgatgagga taaaaattag
      961 gaaaaaaagt taataccaat ttcccggcag taaaggcgaa gcagtgcttc acctttactg
     1021 ccggggcaat gaatcaaaat attcgagggg cgacatttta aaatacttct gaaagttacg
     1081 gctgaaaagg ctttgcgagc tataccctat catctttgaa atctcgtaaa acgaaaattc
     1141 attttgggcg attaaattgg cggccttttt aagtcgcgta atatcaatta attctttcgg
     1201 agacagggag gataaggatt taattttacg atacaacgta gtgcggctca taaacatgtg
     1261 tccggccagc atatcaatat caatatttgg gtctttaata ttctccctga tatattcatc
     1321 cagttttttc agaaaggctt catctgtttt ggaatgcgcc attacccgca catcctcaaa
     1381 aggcgaactg gcaaaatgtt cttttatctt gcggcggttt ttaagcaggt tggcaatctg
     1441 tacctgcagc agctccgacg agaacggctt ttggatataa gcgtctgccc ctacttccaa
     1501 tccttcaatg tgggctttgt aggtattttt agacgttaat aaaatgatgg ggatatggca
     1561 atattcaaca ttagacttaa ttagccggca cagttcaaaa ccatcaattc ccggcatcat
     1621 aatatccgat ataatgaggt gaataatttc tttgtccagc acagtttgcg caatttcccc
     1681 attggcggca agatgcagct ggtagctatc acccaaaact acagataaaa actccaggat
     1741 atcttcatta tcatcaacaa taagaatact ttcggtcata ctactttatc tttttccaac
     1801 tgctcaattg aaactcaaat ttttgatgta tgggcagttt cagttcaaat ataattaaat
     1861 cagtttctcc ggaaatcaat cgcaacgatc cattgtgcag ctcagtgagc gatttggcca
     1921 gcgatagccc gatgccggtt cccggccggt catttccccg tagccttaca aagggttcaa
     1981 atatctggtc tttaaactct gctggtatgc ctttaccatc gttgctaaag ctgatggtaa
     2041 aatattcatc cgtatcgttt accgtattta tactaatcgt ggccctggta gccgcatatt
     2101 taaccgcatt tgacacaatg ttggtacata ttttattgaa cgcttcccgg tctacaaaag
     2161 ccataaaggg ttttcctggt agtactatat ttaaattgat atggttagcg gcagcctgtt
     2221 ctttaaagcc tgctaccagt tcggttatca gcctaacaat atctgttttt acgaaattta
     2281 agctgaactg atctgcttct gtttttctaa aatcgagcaa ctggctggcc agctctatca
     2341 ggcgccgggt atttttttcg gcgattttga gacttttctt gacatgcggt tctttgtcaa
     2401 actttttact caaccactct ataggacctg ctataagcgt cagcggagtt tgtatttcat
     2461 gggtaatatt ggtaaaaaat tcaatcttgg cctggtaaat ttctttttcc ttttcgtgtt
     2521 caaataattg tagtttccgc aaatttttgc gctgcaggta tcggcgatat aaacggtctg
     2581 tgatgaatat cacggtgcct aagagcagta ggtataaaat gtaggcaacg ttgcttttcc
     2641 aaaaaggtgg cagaatttta atgaacagcc gccgctcccg gcctacccag cttcctgtat
     2701 tactttccgc ctgaacaatg aattcatagc tgccgtagct aaggtcggtg aaataggctt
     2761 tgcggttggt gttgagatag gtccagggct ggtcgatgcc tttcatgaga tatttatacc
     2821 gcgtggcccc gggcgatgaa aaatttaatg cggcaaattc aatactaaaa ttgttttgat
     2881 catatttcaa tacaatcgtg tctgtatata aaatagattt gggaagcggg ctgttatgcc
     2941 caccaggcac tgcctctata ttattaatct ggaaacccgt tatataggtc ggcgggctgg
     3001 gctctctttg actaaataaa gccgggtcga aggcaatcat ccctttaaca gagccgaagt
     3061 acattttacc atcggtatgc ttgtacgccg agttatagtt gaattgatct gttatcaatc
     3121 cgttggcctg tgtatatact ttcacctgtt cggtatgcag gtcaaaacaa acaaggcctt
     3181 tggacgaact cacccacaga tgctttgaat tatcttccag gatgctatat aatacattgc
     3241 tgggtagccc gtttgccgta gtgtatcttt tggtggtttt ccggtcgggg ttcagtttta
     3301 tcaacccgga gccagtggtg gcaaaccata aagcatggtc gctgtcttca aagatattgt
     3361 atacagggaa ttcgttgatg gtctttcccc gggtggtatc accaaaacgc aaatttcccc
     3421 gctgtccggt ttcagggtta taataaaatg cgccttcctt tacgctgccg gtccagatat
     3481 ttccggctga atcttcgaaa atatcgtaca cataagtatt gaatggtatc tggctgatac
     3541 gcctaaatgt tttgtgctgt atatcatatt caaatagccc ggcattacga taggcagtac
     3601 ctaccagcag tttattgttt tttgtccggt agatggaaat tacaaaatcg ctcatcggcc
     3661 cggtcttgtc gccgatgagt ttaaaccggt tcgtcaccaa tcctgtacgc acatccatga
     3721 tttccatgcc ctgcaaaaag gggccgataa ataaagcgtt tccccaggaa aacaatccgt
     3781 gtatattcga ataggagatg cttccttttt tgccggtagc tgtatagttt gtaaatagtc
     3841 cggtttttac atccagtttg ctgataccag catcttcggt gccgatccat aagtgcccgt
     3901 ttttatcggc gcatatctca cttatcgcat ttcctgaaat agcattagta cccggcaatg
     3961 gataatactt ctcaaatcgc gcattgactt ttgaataata attcagcccg ccaaaaaaag
     4021 taccagccca cataccaccc tggttatccc tgcatatggc ataaacagca ttgtctgcca
     4081 gggcataggg gtcgcccggt cgttttttca ggctaatgct cttgttgatg gccaggtcgt
     4141 aaatgtaaat tcccgattcg gtggcgatcc agtattgccc atcgttaccc acggcaatgt
     4201 cgcgaacaaa tatgtcctta tgcttatctc catccagtga taaggatttt acgagaccgg
     4261 ttgtagtgtt gtagcttttt aatccctgct taaaacaacc tatcaatagt tcgttgttac
     4321 cgatagggta tattttgctg atagaccggg catttacagg caaacttcta tcaacgatcc
     4381 ttattctgtc gcctggcttt gctttggggt tgtaaatatg aatattaccg tcattatctc
     4441 ccaaccataa attcttattg gaatccagcg caatacagga tgcctttatt ttcaggttct
     4501 ctaccctgtt ttctgcctgt atgtatttgt acagggaaaa ttggatgaga aaccacaggt
     4561 tattgtcatt atctactaca agatggttgg tatattcttt cgggactgtt tccagctccg
     4621 tgaggacttc tttataaggg ttatatttaa aaatgccttt gccggtggcg atccagatca
     4681 tccggttttt atcttcggca atacaattaa tcacatcatt gccgaggctg ccgaatttat
     4741 cgttgttgtt tttgtaggct ttaaaggtat atccgtcaaa acggttcaag ccgccacggg
     4801 tacctaccca tattaaaccg gtactatcct gtattacggc ggttaccgca ttatgcacca
     4861 agccgtcatc tacctggtaa tgcttaaagt aatattggct atagcaggga gaaatagcaa
     4921 ccagtaacag taaattcaac aaaaaaacaa tatgaccgaa ggatctcatc atcactaaat
     4981 ccaaatataa ataatgatat caaaatccta tagggccaaa gtcgaatcat accattgatg
     5041 gccgtacaga ccaccaattt ggtacgattc gtcaaaacat tgaacaaaac ctttatggtg
     5101 cgggatcttc cacaggctct tgatctaccg aataaggatg ttttcccaac gaatatgagt
     5161 ccattattcc cgaatatcca tttaaaggcg gtcgcattaa aaaatactta tatcattgag
     5221 gcagattaca cctacttaaa ataacattga aatgaacaaa aaatttacat tcagtcaaaa
     5281 gccattagac tatgtgtatg ccctcgtgtg gtttggactt tttgtagctt tcgcctacta
     5341 tattttttcc ggtaaaatgt ttcagcccga cgcaaacggg aagattcata tcgtagctat
     5401 tctggtagca tgggtggcag aatatttaac ccagatagga acatccatcc tggttttact
     5461 gataggatta tatatttccg gtaaaatgat tgttaaaaaa gccgtcaact aatcgatata
     5521 tgcaggtcaa ttttatcata ctgctgttta ccggcatata cctggcgggc accctactct
     5581 actacagata tgccgtaaag aaaggaattg cattcaggta taagcctatc accttaatag
     5641 ttgtatttct cctatttctg ctcgctctct atggtattat cacccggcag ccatataatg
     5701 aaattctgcc ctttatacgt tagaagcggg gagctgtttc tttgaactgt taaaatgtaa
     5761 gcagtttacc ctgctccggg atgatcaatt taacatgcag cggattgtaa gcctttagct
     5821 gcgtacggat cacctgctca cggttgccgc caggtttgat atgcgtaatc accaccggga
     5881 aattgtttaa cgcttcctta ccggaaagcg aagcaaggtt ctctaatgag gacattaacc
     5941 aacgtggggt aagatgcccg aataattgtt tatccggttg ttcattagcg aaagagactt
     6001 caataaaaag ggccttcaat ttttttgctt tcagcagcgg cgccacgtat tgccatagct
     6061 gatgcatttt atctgaatgt tctaccgaat cggcccccgt atcgcccagg tacagcgcat
     6121 aggcctcgtt atggcgcacc aggaaagcag tactttgata aggattgcca tgacttaatg
     6181 aaaaagcggt tacctgcata tcggtatttt gcaaaggcat ttctataccc ggcgtaagta
     6241 ccgtataatg gtatttgccc agtgctggtt tctcgccttc gtcggcgaag ttagcccagc
     6301 ttttccacga gaaatattta tccttgagca catccaggca gtagtcaagg ccataaatgt
     6361 ttttgctgct gtcatccggc gagttgatga tcataccagc caggtgatcc aggtgggcgt
     6421 gcgatatgag ataacctttt atatttttct tcaacacctc ttcggccgtg ccgtgcaaca
     6481 gtttggcctg tatggctttc tcaatgccgt aatgtaaggt acctgcgtcg aggcacacat
     6541 agtcattgct accggttgtc gcaatcatgt aggcagatag gttgctttca tcaataccgc
     6601 ccttcacgcc aagcgggatg actttgaacg aagtttgcga aaaagcggtt ttgcaaaaca
     6661 gcacgcatgc gaatagtaga atcgttaaaa caggcttcat cggctaaaga tcaaaatttc
     6721 cgttgaatac tactgatttt ttttatcttt ttcctatgaa acctgttcat cttctttatc
     6781 tgttggccct tgtcaagtta attttaccgt tcttcctcca ggatccaacg tggggtcctc
     6841 accgcgacga attcctttac ctggcagaag cccgtcatat ggcctggggc tatatggagg
     6901 tccccccact gctctctgtc tttgcctggc tcacaaacct cctgggcggc gcaacgtggg
     6961 ccataaaaat atggccttcg ctgggcggcg ccctgacata cgtcctcgca ggccggttgg
     7021 tgctccacct cggcgggcgt tggtttgctc tggtcctggc ctggatgccc ttcgtagcag
     7081 gcgtctggct caggatacat ttcctcttcc agccaaactt cctggatata ttattttgga
     7141 cagcgatggc ctatgggctt accaggtttc agcaatcgga aaagcccata catctttaca
     7201 tggcaggtct tagcttcggc ctgggcatgc ttagtaagta caccatggtc ttttatgcca
     7261 cgggcctgat ggcgggtctc ctcttcacct gggaccggaa gatcctcacc aaccgccact
     7321 tctattatgc gatcgggctc ggcttcttac ttttcctgcc caacctgatt tggcaggttc
     7381 gcaacggctt tccggttgtc taccatatga aagcgcttca tgatggcatg ctccagcacg
     7441 tgagtaactc cgattttctg aaggatcaac tgctatataa tgtggccacc ttgtttacct
     7501 gggtaaccgg cctcatttgg gcagcgcgaa caaaacaata ccgttttatt gcctgggcat
     7561 tcttcgtcac actcaccctc ctgatggtag gccatggaaa aagctattac tcgcaagggg
     7621 cttacccgat cctgtttgca ttaggcgctc cccagctgga ggcctgggca aataaatggt
     7681 ggaagatagg tatgatcgcc tttagtcttt tcataggcat ccgccttacc ccgctcctcc
     7741 tgcctttcaa ggcgcccacg ccactggcag catactatgt tcgtcacccg ttggcgagta
     7801 aagtgggatt cctccgttgg gaagacctcc aggaccactc cctgccccag gatttcgcgg
     7861 atatgttgtc ctgggaagaa atgacccaaa aggtggccaa cgcctatgct tccctcaaca
     7921 acacggagaa ggcccacacg ctgttgttct gcgataacta cggtgaagcg ggagccgtga
     7981 actattatgg cccgaagtac catttgccag ccgcatacag cgacaatgcc agttttcttt
     8041 actggatgcc ccgcgatttc gatcagttcg aagtattgct gctcgtcacg gacgataaac
     8101 aggaaatgca gcatgccttt atcaaagaat tcaaagccgt gcagctcgta gatagcatca
     8161 cgaatcccta tgcgcgggag catggctcgc tgatcatatt gatgaaggga cccagcgaag
     8221 cattccggaa agcattcagg gataaaattg ataaaagtaa actgaaaacc accgcagacg
     8281 gcgctgccca aacgctgaaa cccaacccgc tggaagacgg tggcgcactg cattaactag
     8341 cgcaacttct ttacaaatcc ggcaatggct tcccctattt ctccagggct gtcttcctgt
     8401 aaaaaatgct ttccttttac agttatctct gcctggttag gccaggtgct gcaaaactcc
     8461 cgaaggctgt cattttccac ggcgccgggg ttaccgtgaa tccagagctt gggaaccgga
     8521 ctctctttaa gccagtttcc ataatcagtc actacgtcag ccacttcttt gggcgatcca
     8581 tccaaagcaa tctgacgtgg aaatgataag gtaggacggc gatcctctcc ctcattaaga
     8641 tagggccgcc ggtattctgc catctctgcc tcgctgagtt cccgcaaaat tcctcccggc
     8701 aatacttttt cgataaacac attgttcttc aacaccagct cctcacccgc aggagagcgc
     8761 aatgcacgaa acagatcacg caccttcggt tcaaattcat cccacttaaa aggaatcgcc
     8821 agcgcttcca tatgaacgat accctgtacg cgggaacgat gttgattggc ccagtcaaag
     8881 cctaatacgg agccccaatc gtgcagcacc aacgtcacat tcctttccag tttcaactcc
     8941 tcccacaggg caaaaagaaa attgcgttgc accgaataag catattgctc cggtccggaa
     9001 ccggggagct tttcggagtc gcccatacca atcaaatcgc aggcaataag gcgcccaaga
     9061 ccccggcaat agggcattat gttccgccag agatatgagg aggtaggatt cccatgttga
     9121 aatacgatgg ggtcgccttc gccttcatca atataagcca tacggcggcc ctttatctca
     9181 ataaatttct tttctgcaaa aggtttttcg ctgaaaatgg tctgtacgtt tgctttgtgt
     9241 tccatgtttg taatgatttt atactacaaa gcaacgtcgc tgacatacgt caaaaaataa
     9301 ggtaccttaa gatttcttac gaaacatgtt cgctttcatc tggctaaaga attctggtgt
     9361 aacaccgata taggaggcaa tatatttttg tgaaatccgg ctttcaaggt caggataagc
     9421 cttggtgaat agttcatatc ttccctctgc cgattgtgaa agattggcat ttatcctgtt
     9481 ttgatggaag acaaaagcat tttgaaatag tatcctgaaa aaacgttcga atgtgggcgc
     9541 ttgccggaac aaaatatcca ggttgtcttt gctgatttgc aacagcgtgg taggttccag
     9601 cgcttccagg ttattaatgc tcttttgtcc ggacaaaaaa ctatacaaat caccgctgca
     9661 ccaccaatcc tccggcgcaa acatcaacac atgctccttc ccgctggcgt cgatcgtata
     9721 agtgcgcagg caaccgttga tgacaaaaaa ctgctgcctt gcaacgtcac cttctttcaa
     9781 caaaatttct tttcttttaa cggttttagc aactgcaaga gatagaaaaa tagcagtttc
     9841 ctccggcgtc ggatcaatgt gccgtcgtaa gttttttata atggtatcgt tcattgcagg
     9901 tgacagtcgt tgttttctct aaaaatacaa caaatatcag gtacgaggct gcttctgcca
     9961 gcagcgtatt tggcttcctt tgcatgctta ctttttaata ttaatcgggc aacacggtct
    10021 tttttattgc ctgggcggaa gggaacccct gtaaggaatt gtaccgttcc agccgcgatg
    10081 ataccgactt ccacccactc aatgtcatac tgatatagtg taccctgttt actaccacat
    10141 cctgccattc gccaaaatcg ggcagcgatt gttgcaggcg aacaaactcc ttcagctctt
    10201 cattgtgaat ccggaggtct tccaggcagg cctcttcgag cgaaagattt ttattatact
    10261 ggatggcttt cactacgtta tagtaaatgc tgccggtagc ttcatctttc acaacggact
    10321 gtacttcgtt aaaataaacc atcatccgaa cggcgagtgc tcgtaaacgt tgcagcaccg
    10381 ggtgctcatt aacgccgggg ggtaatggaa taccggtttc caggtctgtt agctccagga
    10441 aagggtacaa acaaagcgaa ttttcccgta tggcaataca ttcttctata ctgggaaaag
    10501 tccttttctg cttatattcc agttcaagtt ccagtcccct gaaatacctg ctgagtcctg
    10561 taataaaacg ccgcaacgat tcttctgaaa tgaattgcag gagttcttcg cggagagaag
    10621 ccagcaatcc ccccagggga atacctgatt gcgcggcatt cacctctcct tttaatactg
    10681 cgatagactg ctggtggata aagcggatct gctcaggcgt tccttcttca tatttgtcgt
    10741 cgttaaagag cgtccacagc ataaatcggc agacgggttt caggcgctcc tggctgctgg
    10801 taggaaacca ttgcgacgca atatgggctg tctttgtttt cttgtatttc tgacgggtct
    10861 gcggatcatc tttatatagc caaagatatt ccccatcgat ccattggttg tcggtaatct
    10921 cctgcaaggc atctgcaaaa ggatttttca gcgtgggaaa tgggtaactg aacagggaaa
    10981 gcatttttgc attcatggtt attttgattg agggtaaaca aattaatatc cagctagcat
    11041 ggatagcaat ttgcgctgaa tagggttctc atactattat atggggatat gaagataatt
    11101 ttcattactg caatataatc gcagtaacaa aaagttcgga aggaagctca gccgccgcta
    11161 ccgcgtttat gaccacgccg tttctccatt ataaggaata catccttcat atttctccgg
    11221 caccggaaga tagcgcagca cttgagttgc gccggcttta gaagtaaaga aacccagtaa
    11281 ggtgagctgc ttcattaacg taaaataatg cggcgcatcg tcgttgttat gctggcgctt
    11341 ggctgcttca tcgataccgg tcagcagcga ttgcttttgt ttcgtaggaa gttgtacaaa
    11401 agcctgcttg aacttctcct tcgacgccgt atttagctgc gtaataccct gttttaacaa
    11461 ctgctgttgc gtgttatcat aacaaccggc agcatataaa gccatgaact tacccacttc
    11521 ggcgtctttc gctcccggcg tgtcggtacg gggaataatg gcttctgcca cctcatccaa
    11581 aaaggcgatg tcatcactgc tgaaaagagg ccccttttgt tctttacatc cggagagaaa
    11641 tagttcgccg ccaacaaatg ctgccccagt cagcacggcg attgttttga ttaattcgcg
    11701 acggtccatt taaaacgctc ctttttttag ctgatccact gcataggtag cagctctcgc
    11761 ggttaatgac atataaagaa tagaggggct ttgattgcct gtactagcca tacaggcgcc
    11821 atctgttacg aaaacatttt tgcaatgatg caactggttc cattcattca gtaaggaagt
    11881 agcagggtct ttacccatgc gtaccccgcc catttcatgg atatccagtc ctggcggctg
    11941 atggttgtcg tacaccgcaa tattcttggc ccccgcagcg gtgagcatgg cttttccctg
    12001 ttcgataaaa tcctttacca tccggtcgtc attgtcgtca taatctacgg aggtgatcag
    12061 caagggaatg ccccattcat cttttttatc tttgctcagc cttacatgat tgctttcctt
    12121 gggaatcgtt tcgccttgca tatacatgta cgcgccccat ttccctggtt tgctgatcgc
    12181 ttctttaaac ttcgccccta ccgcagccgg caggttttta tcgcttaact tttcacgata
    12241 agcagcactg aagatgacat agccgcctac gaagtcggtg tctttctcat acacattgcg
    12301 gaagttggga ataatacatt ccgacggttt agatacttta tagtatttat cttcaaagcc
    12361 gtcgaactcc ccgctcatcc cggcccggta gttatggaag cagatgtatt tccctaacaa
    12421 tccgttgtcg ttccccaggc cattcggaaa ccgtttggag gtagagttca gcaaaacaag
    12481 gttggtattt aaagcagagg cattgaggaa aataattttc gcatggaaaa ccatcgcttc
    12541 ttttgtttta gcatcgatca cttttactcc tgttgccttg ccggactgct catcatagat
    12601 aatagaatgc actacagaga agggccttat ggtgaggttg cctgtttttt ccgcccaggg
    12661 aagtgtagac gcgttggagc taaagtagcc gccaaagggg catccacgca tacagagatt
    12721 gcggctcagg cactggcgat gctgttcttt ttgaacctcc tgcggctgcg ttacctgggc
    12781 ccatctgccg gatacatagt gacgattgaa cttttctctt accacatcgc ttaaatgctg
    12841 ttctacacaa ttcaattcaa atgctggctg gaattctcca tccggcatcg tagcaatgcc
    12901 ttccttgttt ccacaaacac caatgaattt ttcgacgtgg ctataccagg gcgcaatgtc
    12961 gtcatagccg atgggccatt caattccata cccatagcgg tgcggggcag taaaatccat
    13021 ttcgctccag cgggcacagg cccgtcccca tatgagcgat tttccaccca cctggtaccc
    13081 acgtatccat tcgaaaggtt tttcctggat ataaggatgg tcggcgtctt ttacaaaaaa
    13141 atgtgcggtg ctgtcgtcat atcctgccgc cctgctgata taaggattgg ctttcaatac
    13201 ttctttggat actgcgccac ggtacttaaa ctcccaggac ggggtattgg ccgtaggata
    13261 gtcctttaca tgttcaacat ttctcccccg ttccaacacc aatgttttca ttcctttttc
    13321 acacaattct tttgcagccc agccaccgct gataccactg ccaatcacaa tggcatcaaa
    13381 ctgcttaaca gcatctgaat tatcggccat acataaaaat tttattggtt attcattgag
    13441 tatgggcgcc cgtcggcagc tgacaaatac aatgcagccg cgcctaaaat attaccattg
    13501 attaaggaag atgtttcaat cttcagcgcc gccagggaac gactgaattc aaaatccccc
    13561 agtgttgccc acatactttt tgcgtaaagc gggaaagctg ctttcacagc accaccgatg
    13621 ataatacatt ccgtgtccag cacatacatg atcaacttaa tagcctctcc caaatgccgg
    13681 ccaaaatcgc cgtacatttt tattgcttcg ggatcacctt ccagtgcccg cctgtacgcc
    13741 tcttccccag ctatttgata tacatttgaa aaaaaacgtc cactgacata atactcgata
    13801 tttttccccc cgtagggtat cataccaaat tcacctgctc ctccatgctt gccggtaaat
    13861 aatttcccat cgacaataat tcccgctccc agtccggttc ctatcgttaa tcccaccagg
    13921 gaagtgcagg aacgcccggc gccgaaatga aattctccca atgcaaaaca gttggcatca
    13981 ttttcaattt tcacggctac ctggtagcgc tgctccaaca cagtccggag atctgtttca
    14041 ttcaacgatg gaatgttcac gatatcatag ggtatcctgc tgccgggatc taccagtcct
    14101 ggaacaccta tcccgatggc ttttaccccc gggctgatga ctttgtcgat gacgccaaaa
    14161 atctgctcca ggatttgcgc cctgcttccc tgtgcataaa ggggttgcgc cacaacagac
    14221 tcgcaaatgc cttcattgac aatgccggca cgtaaattag ttcctcccag gtcaacgcct
    14281 attatctgcg ttttctccat atcattgctt atttgataaa gaggctgccg cagctgcatc
    14341 caagaaaaca gttaccgatg gatgctgtaa cagcaaactg gccggcaact cattcgttac
    14401 agactcctcc agcattttac ggacaatctc cgctttgtgc tggccgctca ccattaaaat
    14461 gatatgcctg ctttctgaga gcgtccccat tcctaaggtg attcctttgt ccaatacctg
    14521 tggctggcta aaatacttct gacctacctg cttcgttacg ggatgcaatg ttattacctg
    14581 tgttctccca tctatggcgc atccgggttc attcatggca atgtgtccat tcatgcctac
    14641 gcccaggaca gccacatcta ttccattcct ttcacgaata aactgctccg ctgccgaaca
    14701 ttctccccga agatccgcgg agcggccgtc aaagaagtaa atgttatttt cggaaacatc
    14761 gagtggccgg aacaattctt tgtcaatgga atagcggcaa ctgccctcgt ctgttccatt
    14821 aagtcctacc cactcatcca accctacaaa gctccagtct gtcgcctcat attgctgctg
    14881 ctcacgtttt tccgccagcg ccttatataa gcccgccggc gtatcgccgg atgccgggca
    14941 tagcagcggc ttttcatgcg ccgacatcag ctgtatcacc tgctccgcta cgtgtgccga
    15001 aagctcggca tatgtttgat agattgctat tttcacccgc ctggttattt agtggtggat
    15061 gttgggtgca accataggtc aataggcttt gctacctgct ggttaaaggg aagcgttatc
    15121 cgtttgccct gccctgccga tgcataggca gcatagataa tttccagcac cgctcttcca
    15181 tcttttcctg ttaccagcgg ttcgcggtca tgctgcacac attcaataaa atgtttcaat
    15241 tcctgcggat atccctggtt aaaggcttct tcgaagatgc aaaagctcca tccaatagta
    15301 gtatctgtct tttccatagc atacccatag ccatttttac tgtaggccag cacagagttc
    15361 ccggtaaaga gatcggcata agccacaccg tccaggccat gaatttcact gcggtcatcc
    15421 ataccgccct gtttggccca ggagttttct acaacgccag tcaccccatt ttcaaattct
    15481 atgatcacca cggaattatc ttctcccctg gtcctgttct tgtggaatac tgtcttcata
    15541 gatgcggtaa cacttactgc tttgctgttc ttcaacatcc accggaacca ggcgatgcca
    15601 tggcagccca tatccatcaa tacaccgccg ccggattggc tcacatcgta aaaccaatcg
    15661 gaatgcgggc cagagtgttt ctctgactgc tttaacatat aaatctcgcc caccgcgcct
    15721 tcttctacca gtttccgggc gcgttcatat ttaggcgcaa agcaaagttc ttctgcatac
    15781 atcagcttta cattggccgc ttcgcaggct gcaatcattt catctgcttc ttccagcgta
    15841 accgccaggg gcttttcaat aataatgtgc ttccctgcgg cagcggcttt cagcgtcgct
    15901 tccttatgca ggaaattagg gagacaaata tctacgacgt ccacttcaac ctgttgcaac
    15961 aactcatcga tggaagaaaa atgctgtgca atatgatgcc tgtctgcaaa ggcttttgct
    16021 ttttcagcat ttctcgtata cacggctact acttccgctt ccggaacaaa ccgggtataa
    16081 ctttccatat ggatatctgc aataaatccg gctcctaaca gggcgacctt cactcgtttc
    16141 atagtttaat gttaaagtgg ttcactaaaa atattattga tggtagctgt ttgcaacctg
    16201 atgtcatcag acgcacttcc aatcatgatc tggcatgcta caggcagtac aactttctct
    16261 aatttttcat tgtaagaaca gcgttcatct tttgtaaata ctatggtaac tgctttagtt
    16321 tcgcccggtt gaggaaaact ttcttaaacc cttttaattc atttacggga cgcagtggcc
    16381 tggatacaat attatgaata tacaacaggg aagtttctta tcctgtataa ccacccgcat
    16441 ttttttcctg gaaggacact tttatgcttt cttttccggc tactttccat ttcagatcgc
    16501 ttcgtacatt cttacataat ccacctgcat agctgcaggg aataccgctt catccacacc
    16561 ttcctttccg ccccagttgc cacctacagc cacattcaac agcaaataga acttcttgtc
    16621 aaacggccat cctttatgac ctttgtgttc attggccacc tggtaataca ctttcccatc
    16681 cagcaggaag gtgatatcat caggggtcca ttctattgca tacacatgaa acttgtcgta
    16741 gggttgtttt atataaacac cttttgtttt ctgtgttccg ataacgtggt tataggtttt
    16801 gctgtgcaaa cttccaaaga tactgtcttt catatagccc acatgctcca ttacgtcaat
    16861 ctctccgctt tccggccagc caccgtattc gtcgcccgtt gacaacatcc agatagcggg
    16921 ccaaagacct cttcctttgg gaagcttcgc acttacctcc agttttccat atttccagtc
    16981 gcctttgttt ttggtaatca ttctggctga agtatattgg tgatctccgc tcccgccatt
    17041 cctggcggta atagacaatt tacctttttc cactttcgca ttattagtat ctgcggccgt
    17101 gtaaaactgc agctcattat ttccccagcc ggatctcccg gtttgatacc cccatttggt
    17161 gctatccggc aatcccttgt agttaaactc atcactccag gtcaatttcc atttcgtatg
    17221 acgtttaatg gattgggcct gggatgttgc gataaagcaa catcccaccc aggctaaaac
    17281 gagacaatag atacttcttt tcattagatt tagcgctttc tttattatca ttaaaattac
    17341 ttcaacaggg aattgatatc ttcctgtgcc tgtggaatcg gaatgtattc actttttcct
    17401 tttataaatc ccgggagtac ggtagccgcc tggtcggttc tcaacaggtc gttaaaccgc
    17461 tcgccccacc attcacagat cagctccgca tggcgctcgt ccaacagctg ttgcagggtt
    17521 acaccattaa tagaggccag ctttacacgg tttctgacca ggtttacctg ggcatcgccg
    17581 ctttgtcctt tacggatttt agcttctgca ttcattaaca gcgcttctgc ataacgcatg
    17641 atccgaacgt tgttattggc gccataataa tcaaccctgc ccggtgtcat ttgtgagcgt
    17701 ggcgtatatg atttgccatt aaaatatttc ttcctggatc cattcccgga gatcgtatcg
    17761 ccatccggcg taactgaata ggtgcccgga gcgccattaa caccacaata ctggatcgcc
    17821 atttttaaac gcacgctatc attacgtgat ttcaggaaat caaccgcttt ttgtgtgggc
    17881 accatccaac ctggaccggt aattactgaa ctgtaggtat tttcaggacc ctggaaaaaa
    17941 aagaagttgc cccattcttc gccgggaccg ccggaaatca cgatatcgcc ggtagatttt
    18001 ccaaaatcag aatattgtag ttctaaaatg ctttcatcac acaacttacc tggcatttta
    18061 aacagctggt agtaattact gaacagggag aatttaccgc tgttcacgat ctggttggta
    18121 caatccagta ccacatccca ataggtacta ccattgttgt tacctgccag gtccatagct
    18181 gctttggctt tcagcatcag tgcagtatat ttggtcaccc cgccaacgtg cgcagcagca
    18241 ttgggccggg catcttccag gttgggaata cagaaatcca tttctgccat cacatactta
    18301 ttcacgtctt ctaagctgct cttcccaacg gatcccagct tagccggatc gatggtttcc
    18361 acccccagga tgggtaccgc gccaaagctt ctgcctagcc agaactgggc aagcgctcta
    18421 tagaagcgta cctctgcctg gtatcttgcc aacagtttgg ttttgtctgc atcagaagcc
    18481 gggatatgct ttccaaattg gtccagttcg tataaggcgt tatttgcagc aattaccacg
    18541 gcatacatgc tgatccacat gtcgttgata ccccagtagc tttttacggt gacatctgtt
    18601 tgaaaattat ggatgcctat ttgtccggcg tcatcatttc ccggctcgat atcatcgcta
    18661 cgggaagacc gaatggccat gctgatccat tttccaaaag taccgctggc tgcggttcta
    18721 tatacaccag acactggctg gtacattaat gacaaatcag tatagtcgat attggttgcc
    18781 tgcgccttat tttccaatgg gcgatccagg aacttggagc aaccggaaga taaaagcagt
    18841 acgatgagca agattgatat gtagtttctc atgttttctt ttttaaattt tctataacgg
    18901 gtatcaagga aacttttcgt gtatccttat aagtgagctt tccttagaaa gccgctctaa
    18961 cacctaaact gtaggaagaa gaaaccgggt atacattggc atcatagcca gacaccgggt
    19021 aaccgccagc cgtattggta cccacttcag gcgtaaagcc gttgtaacgg gtaaagatga
    19081 atggtctgtc ggcggtagca aataccctca tagccacagg cgattgttta cccacattaa
    19141 agttatatcc cagctgaatg ttctgtacac gcaggtaagc accactttcc acaaagaagg
    19201 agttgctcac tttaaaccaa ccttgtccca gggcataagc agaaggatag gcattggttg
    19261 atcccgggcc agtccacagg tttgaggcaa atttttcatc caggttcagg gaagtggaag
    19321 ctttgtaaag ccgtccccta ttgaggttga agattttatt gccagatacg ccctggaatg
    19381 ccacgctgaa gtcgaacttc ttatacgtaa gtgctatatt gaaaccatag gtaacctttg
    19441 gtaaatagct gcccatgttg atacggtcgt tattatccaa taccccgtcg ccgttctgat
    19501 ctttatactt ggggaaacca ggaacaattt tcgtggcagc tgtctggttg tagttcttcg
    19561 caatcgggtc attgttgatt tcgtcctgcg tttgatatac gccggtaaat tcataaccat
    19621 agaaatagtt gatcggttgt cctacaacga tacggttggg gaattctgca gtccattcag
    19681 ggaagccgct ggtagaactg ggaagcgttc ccaaatcggt caccttgttt ttcagggtcg
    19741 acatattcgc gccgatctgg tatcccagct caccgatttt atcattccag ctcaatgcca
    19801 catcccaacc actgttcacc atacttgccc agttgccata aacatttctg taggtggcaa
    19861 agattggttt gttgaaggct gcctggctag ttttccggtt gaaatagtca acagagccct
    19921 ttaatcttcc cctcagcaac tcaaagtcaa caccaccatc ccattcagtg acaacctccc
    19981 aagtgataga agtaaagtac ctgtctaccg tatatccagg tacatatacg ccattagcac
    20041 ccgcggtgct accaaaaacc gcggagttac tgttacccgt tttgacgata gcgtaagcgg
    20101 cattgggatt taccccgtca tttccaagtt gtccccagct acctctcagt tttaagaaat
    20161 cgaagatttt ctggtttttc ataaatgcct ccctgctcaa tacccatcca aggcccactg
    20221 atgggaaata tccccatttg gtctggtatt tagaacttcc atctgcgcgg aaggtggccg
    20281 tcagcaagta tttattgtcg taatcatagg tgccacgtgt aaaataggaa aggccggcat
    20341 tacgggtacc atcttctcca taataagctg cgctcggcgt gccctggctc gcgtaccacg
    20401 cttcttccac attcggtaca tcgtcggctt cggtccgtgt ctgacgccac cgctcttccc
    20461 tggtagattg tcccagtaac aggctccagt gatgtagccc tttgctgtcc cgataggtaa
    20521 gcaggttatc caatatataa ttggtggttc tttcctggat cgatctcaga tgagatttaa
    20581 cagcgccatc agcaccgggg cccaggttcc tggcaggcag gtagtttgtt cccagcagcg
    20641 aaccatagat ctcgctcaac tgggacctga aagtgagttt attctgccag aaactggctt
    20701 cgccatatat agtcggtaat atctggaaag cctttgaccg gtcataatta taataggcgg
    20761 aggccatcgg gtttttatca tccgatcttc caatcacaga agaggcagtg aacttcaccg
    20821 ggaaggcatt cacgttggcg ttgtcataaa ccgggtacaa aggagaagcc gtataagcat
    20881 ccaggaaagc gccataattg ggattaaacg tagtagaatt attaaagata gcggagaagc
    20941 ctactttcag ccagctaaac gccttcgctt ccagttgtaa ccgtccatta tatctt
//
