LOCUS       NZ_JAASAG010000029      2809 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. HNM0575 scfid29, whole genome shotgun sequence.
ACCESSION   NZ_JAASAG010000029
VERSION     NZ_JAASAG010000029.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. HNM0575
  ORGANISM  Streptomyces sp. HNM0575
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Xiao,K.
  TITLE     New actinomycetes from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Xiao,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) College of Tropical Crops Hainan University,
            Hainan University, Renmin Avenue, HaiKou, HaiNan 570100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2018
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012726265.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 05:29:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,992
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 69
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 15 of 126
            Pseudo Genes (incomplete)         :: 115 of 126
            Pseudo Genes (internal stop)      :: 5 of 126
            Pseudo Genes (multiple problems)  :: 8 of 126
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2809
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASAG010000029.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2809
                     /collection_date="2018-06-19"
                     /country="China: HaiKou"
                     /db_xref="taxon:2716338"
                     /host="sponge"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. HNM0575"
                     /strain="HNM0575"
                     /submitter_seqid="scfid29"
     gene            <1..>2809
                     /locus_tag="HCC61_RS29985"
                     /old_locus_tag="HCC61_29980"
     region          1..2809
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2809
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2809
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:>2809](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2809
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             1..>2809
                     /NRPS_PKS="Domain: PKS_AT (292-594). E-value: 6.3e-92.
                     Score: 300.0. Matches aSDomain:
                     nrpspksdomains_HCC61_RS29985_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="HCC61_RS29985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29980"
                     /product="type I polyketide synthase"
                     /protein_id="WP_169141009.1"
                     /sec_met_domain="mod_KS (E-value: 3.5e-84, bitscore: 269.1,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.1e-93, bitscore: 300.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTAPNGPSQQRVIRQALSAAGLAASDVDAVEAHGTGTALGDPIE
                     AQALLSTYGQERESDRPLLLGSVKSNLGHTQAAAGVAGVIKMVEALRHGELPRTLHVDS
                     PSSHVEWDAGAVELLRERVAWPQVGRVRRAGVSSFGISGTNAHVILEQAPVSEPGGESD
                     SVPDSSDSVSGSPDSVSGSVSVLPDSDVPGSGFGGVVPWVVSGRSEGALDGQLGRLESF
                     VGGVSGGVRPVDVGFSLMSSRSVFEHRAVVVREVESGEGSVSDSGGVSSSVSSSVCVRG
                     VVGEVGSGPVFVFPGQGSQWVGMAVELLDSSPVFGWWMGECDRVVGGLAGFSVVGVLRD
                     SLGGGAGVSVLERIEVLQPVLFSVMVSLAELWRSCGVVPSAVVGSSQGEIAAAYVAGVL
                     SLEDAARVVVLRSGLFARELVGGGAVASVALPVGRVRGLLEGWSGRLSLAGVNGPSAVT
                     VAGELGALEEFVSVCEERGVRARVVGSSVASHSVQVEPLREELLGLLEGVECVSGGVPL
                     YSTVSGGVVDGGELGVEYWFANAREPVDFAGAVRSLVGDGYRVFVECSAHPVLMGAVEE
                     TAEESGVEVVALGSLRRGQGGGVRFVTSLAEAWVRGVPVEWGRLFEGSGARSVELPTYA
                     FQRERFWPKAPVVVEGGSHPVDAALWGLVESADDDVLARVLGVDSVTARQVASGLVEWR
                     GRERVRSRTGGWCYRESWKPLNLNRIDTTSLNSNGSTPESPDADSAVSGVWLVVVPAGG
                     GVPVGWVSLVVGLLGAGVVCLEVEEGVERAVLAARVGEVLPSGGECAGVLSFLPVGDGG
                     VGSLSSGVSGVPGGLWGSAVLVQALGDAGVVGPVWGVTCGAVAAVPGDVVVHPVQAAVW
                     GLGRVAALELPHRWGGLVDLPADPDSWNEEIGRGVAGLLASGAGEDQVAVRDTGIYGRR
                     LVPTEL"
     CDS_motif       91..135
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0001"
                     /evalue="2.10E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="45"
                     /protein_start="30"
                     /score="13.3"
                     /tool="antismash"
                     /translation="VEAHGTGTALGDPIE"
     CDS_motif       196..240
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0002"
                     /evalue="6.90E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="80"
                     /protein_start="65"
                     /score="12.1"
                     /tool="antismash"
                     /translation="GSVKSNLGHTQAAAG"
     CDS_motif       871..927
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0003"
                     /evalue="4.70E-10"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="309"
                     /protein_start="290"
                     /score="31.0"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWVGMAVELL"
     aSDomain        877..1782
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: False"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HCC61_RS29985_PKS_AT.1"
                     /evalue="6.30E-92"
                     /label="HCC61_RS29985_PKS_AT.1"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="594"
                     /protein_start="292"
                     /score="300.0"
                     /specificity="substrate consensus: pk"
                     /specificity="PKS signature: Ethylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWVGMAVELLDSSPVFGWWMGECDRVVGGLAGFSVVGV
                     LRDSLGGGAGVSVLERIEVLQPVLFSVMVSLAELWRSCGVVPSAVVGSSQGEIAAAYVA
                     GVLSLEDAARVVVLRSGLFARELVGGGAVASVALPVGRVRGLLEGWSGRLSLAGVNGPS
                     AVTVAGELGALEEFVSVCEERGVRARVVGSSVASHSVQVEPLREELLGLLEGVECVSGG
                     VPLYSTVSGGVVDGGELGVEYWFANAREPVDFAGAVRSLVGDGYRVFVECSAHPVLMGA
                     VEETAEESGVEVVALGSLRRG"
     CDS_motif       1051..1122
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0004"
                     /evalue="1.80E-10"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="374"
                     /protein_start="350"
                     /score="32.8"
                     /tool="antismash"
                     /translation="LERIEVLQPVLFSVMVSLAELWRS"
     CDS_motif       1126..1236
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0005"
                     /evalue="1.00E-17"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="412"
                     /protein_start="375"
                     /score="55.9"
                     /tool="antismash"
                     /translation="GVVPSAVVGSSQGEIAAAYVAGVLSLEDAARVVVLRS"
     CDS_motif       1681..1710
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0006"
                     /evalue="4.40E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="570"
                     /protein_start="560"
                     /score="9.8"
                     /tool="antismash"
                     /translation="FVECSAHPVL"
     CDS_motif       1945..1968
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0007"
                     /evalue="9.90E+01"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="656"
                     /protein_start="648"
                     /score="-3.3"
                     /tool="antismash"
                     /translation="VEGGSHPV"
     CDS_motif       2581..2613
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29985_0008"
                     /evalue="9.00E+01"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="HCC61_RS29985"
                     /protein_end="871"
                     /protein_start="860"
                     /score="-3.0"
                     /tool="antismash"
                     /translation="VVVHPVQAAVW"
ORIGIN
        1 ggtttgacgg cgccgaatgg tccgtcgcag cagcgggtga ttcggcaggc gttgtcggct
       61 gcgggtcttg cggcgtcgga tgtggatgcg gtggaggcgc atggtacggg gacggcgttg
      121 ggtgatccga tcgaggcgca ggcgttgttg tcgacgtatg ggcaggagcg ggagtcggat
      181 cggccgttgt tgttggggtc ggtgaagtcg aatctgggtc atacgcaggc ggctgcgggt
      241 gtcgcgggtg tgatcaagat ggtggaggcg ttgcggcatg gtgagttgcc gcggacgttg
      301 catgtggatt ccccgtcgtc gcatgtggag tgggatgcgg gtgcggtgga gttgcttcgg
      361 gagcgggtgg cgtggcctca ggtggggcgt gtgcggcgtg cgggggtgtc gtcgttcggg
      421 atcagcggga ccaatgccca tgtgattttg gagcaggccc cggtttccga gcccggtggg
      481 gaatccgatt ctgttccgga ctcctctgat tctgtttccg gcagccctga ttctgtttct
      541 gggtctgttt ctgtgcttcc ggattcggat gttccgggtt ctggttttgg tggtgtggtt
      601 ccgtgggtgg tgtcggggcg ttctgagggt gctttggatg gccagttggg tcgtttggag
      661 tcgtttgttg gtggggtgtc ggggggtgtg cggccggttg atgtcgggtt ttcgttgatg
      721 tcgtctcggt cggtgtttga gcatcgtgcg gtggtggtgc gtgaggtgga gtcgggggag
      781 ggctctgtct cggattccgg tggggtttcg tcgtcggttt cttcttctgt gtgtgtgcgg
      841 ggggttgtgg gtgaggtggg ttctggtccg gtgtttgttt ttccgggtca gggttcgcag
      901 tgggtgggga tggcggtgga gttgttggat tcttcgccgg tgtttgggtg gtggatgggt
      961 gagtgtgatc gggttgttgg ggggttggct ggtttttctg tggtgggggt gttgcgggat
     1021 tcgttgggtg gtggtgcggg ggtttctgtg ttggagcgta ttgaggtgct tcagccggtg
     1081 ttgttttcgg tgatggtgtc gttggcggag ttgtggcggt cgtgtggggt ggtgccgtct
     1141 gcggtggtgg ggtcgtcgca gggggagatt gcggcggcgt atgtggcggg ggttttgagt
     1201 ttggaggatg cggcgcgggt ggtggtgctg cgttctgggt tgtttgcgcg tgagttggtg
     1261 ggtgggggtg cggtggcgtc ggtggcgttg ccggtggggc gtgttcgggg gttgttggag
     1321 gggtggtctg gtcgtttgtc gttggcgggg gtgaatggtc cgtcggcggt gacggtggcg
     1381 ggtgagttgg gggcgttgga ggagtttgtt tcggtgtgtg aggagcgggg ggtgcgggcg
     1441 cgggttgtgg ggtcgtctgt ggcgtcgcat tcggttcagg tggagccgtt gcgtgaggag
     1501 ttgttggggt tgttggaggg ggtggagtgt gtttcgggtg gggtgccgtt gtattcgacg
     1561 gtgtcggggg gtgtggttga tggtggtgag ttgggtgtgg agtattggtt tgcgaatgcg
     1621 cgggagccgg ttgattttgc tggtgcggtg cggtcgttgg tgggtgatgg gtatcgggtg
     1681 tttgtggagt gcagtgcgca tccggtgttg atgggggctg tggaggagac ggctgaggag
     1741 tcgggtgttg aggttgtggc gttggggtcg ttgcggcgtg gtcagggggg tggggttcgt
     1801 tttgtgacgt cgttggctga ggcgtgggtg cggggtgtgc cggtggagtg gggccggttg
     1861 tttgagggct ctggtgcgcg ctcggtggag ttgccgacgt atgcgtttca gcgggagcgt
     1921 ttttggccga aggctcctgt ggtggtggag ggtgggtcgc atccggtgga tgcggcgttg
     1981 tgggggctgg tcgaatccgc cgatgatgat gtgctggcgc gggtgctggg ggtggattcg
     2041 gttacggcgc ggcaggtggc gtcgggtctg gtggaatggc gcggtcgtga acgcgtgcgt
     2101 tctcgtacgg gcggctggtg ctaccgcgaa tcctggaaac ccctcaacct gaatcgtatt
     2161 gacaccacct ctctgaatag caacggttcc acgccggaat ctcccgatgc cgattctgct
     2221 gtttccggtg tgtggttggt ggtggtgcct gctggtggtg gtgtgccggt ggggtgggtg
     2281 tcgttggtgg tggggctttt gggtgcgggt gtggtgtgcc tggaggttga agagggtgtg
     2341 gagcgggccg tgttggcggc tcgtgtgggg gaggtgcttc cttccggtgg ggagtgcgct
     2401 ggtgtgttgt cgtttttgcc ggttggtgat ggtggtgtcg gttccctgag ctcgggtgtg
     2461 tcgggtgtgc cgggtgggtt gtggggttcg gcggtgttgg tgcaggcgtt gggggatgcc
     2521 ggtgtggtgg ggccggtgtg gggtgtgacg tgtggtgcgg tggctgcggt gccgggggat
     2581 gtggtggtgc atccggtgca ggcggcggtg tggggtttgg gtcgtgtggc ggcgttggag
     2641 ttgccgcacc gctggggtgg tttggtggat ctgcccgccg accccgactc gtggaacgag
     2701 gagatcgggc gtggtgtggc tgggctgttg gcgtctggcg cgggtgagga tcaggtcgct
     2761 gtccgcgaca cgggtatcta cggccgccgc ctcgtaccca ccgaactga
//
