LOCUS       NZ_JAASAG010000028      3090 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. HNM0575 scfid28, whole genome shotgun sequence.
ACCESSION   NZ_JAASAG010000028
VERSION     NZ_JAASAG010000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. HNM0575
  ORGANISM  Streptomyces sp. HNM0575
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Xiao,K.
  TITLE     New actinomycetes from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Xiao,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) College of Tropical Crops Hainan University,
            Hainan University, Renmin Avenue, HaiKou, HaiNan 570100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2018
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012726265.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 05:29:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,992
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 69
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 15 of 126
            Pseudo Genes (incomplete)         :: 115 of 126
            Pseudo Genes (internal stop)      :: 5 of 126
            Pseudo Genes (multiple problems)  :: 8 of 126
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3090
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASAG010000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..3090
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:3070](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3070)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            complement(<1..3070)
                     /locus_tag="HCC61_RS29980"
                     /old_locus_tag="HCC61_29975"
     CDS             complement(<1..3070)
                     /NRPS_PKS="Domain: PKS_KR (383-552). E-value: 3.3e-49.
                     Score: 159.2. Matches aSDomain:
                     nrpspksdomains_HCC61_RS29980_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (657-729). E-value: 1.1e-30. Score:
                     97.7. Matches aSDomain: nrpspksdomains_HCC61_RS29980_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (751-1023). E-value:
                     1.9e-111. Score: 364.9. Matches aSDomain:
                     nrpspksdomains_HCC61_RS29980_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="HCC61_RS29980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29975"
                     /product="type I polyketide synthase"
                     /protein_id="WP_169141007.1"
                     /sec_met_domain="PKS_AT (E-value: 1.9e-23, bitscore: 69.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 6.4e-53, bitscore:
                     165.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 8.4e-11, bitscore:
                     27.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.7e-09,
                     bitscore: 23.3, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.6e-16, bitscore:
                     46.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 8.9e-138, bitscore:
                     445.7, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 7.3e-94, bitscore:
                     300.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDEFAGVVAGLEFHSPRIPLVSMVSGERVEALGPEYWVEHVRVA
                     VRFADTVQALAAEGVSTVLELGPDAVLSGMGQQLLPPDGSFIPALSQGEHEPGEPAALL
                     TALARLSVRGADVDWSELFPAARRVDLPTYPFQHRRYWLEDTSADTAWWDGLDLDDLPD
                     DLRTSLEPALPALAELRRRTENKSTVDGWCHQVAWVPVAGRSAGSGKPAEPTAPAEPTA
                     PAESGTWLVVQPPGFRPPAGIPEGSTTVELDTVDRAEVCERLRGLRPDRVVSLLALGDA
                     RRSGATLLALVQGLGDAGIDAPLWCVTTGEPLQALAQGLGRVVAAEEPGRWGGLVELIG
                     DDAWARLPEAVAGPEHEVSVRSEGSYVRRLVNAPGAAGGWEPSGTVLVTGGTGALGRRV
                     AHWLAAEGADRVVLVSRSGPEAPGAADLTALGVETAVVAADLTDPERARELMAEWRPTA
                     VVHAAGVLDDGVVDSLTPDRFEAVVGPKADALENLRQAAADETVFVVFTSVAGVVGSPG
                     QGNYAAANAACDALVASYRDQGLRAVSVAWGPWAEDGMAEGKRLGLDGLSPMPPHLALS
                     VLRRAAAPLTAVPSAASPMLAAPTAAASLLVADADWPLLAGQLASSQLFDELPGVSRTA
                     DVEAGASRFAGLSEAERRRAVLSLVRDETASVLGHGDAGDLRVDRPFRELGFDSLRAVQ
                     LRNRLAAATGLTLSSTIVFDHPTADDLTAYLEAELSGDTDTLEAASAGSGRAVVDEAVA
                     IVGMSCRFPGGVGSPDELWRLVDEGVDAVGAFPDDRGWSLSDIYDADPDRAGTTTVREG
                     GFLHDAGDFDAEFFGISPREAAVTDPQQRLLLETAWEAFENAGLAPASLRSGDVGVFVG
                     SNGQDYGSRVLAAGEEAAGYGATAASASVMSGRVAYALGLQGPALTVDTACSSSLVALH
                     LAAQSLRNGECGLALAGGATVMATPYTFVEFSRQRGLSSDGRCHAFSADADGTGWGEGA
                     GWLVLERLSEAERNGHRVLAVVRGSAVNQDGASN"
     source          1..3090
                     /collection_date="2018-06-19"
                     /country="China: HaiKou"
                     /db_xref="taxon:2716338"
                     /host="sponge"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. HNM0575"
                     /strain="HNM0575"
                     /submitter_seqid="scfid28"
     region          1..3090
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..3090
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        complement(2..817)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HCC61_RS29980_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.90E-111"
                     /label="HCC61_RS29980_PKS_KS.1"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="1023"
                     /protein_start="751"
                     /score="364.9"
                     /tool="antismash"
                     /translation="VAIVGMSCRFPGGVGSPDELWRLVDEGVDAVGAFPDDRGWSLSDI
                     YDADPDRAGTTTVREGGFLHDAGDFDAEFFGISPREAAVTDPQQRLLLETAWEAFENAG
                     LAPASLRSGDVGVFVGSNGQDYGSRVLAAGEEAAGYGATAASASVMSGRVAYALGLQGP
                     ALTVDTACSSSLVALHLAAQSLRNGECGLALAGGATVMATPYTFVEFSRQRGLSSDGRC
                     HAFSADADGTGWGEGAGWLVLERLSEAERNGHRVLAVVRGSAVNQDGASN"
     CDS_motif       complement(107..145)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0006"
                     /evalue="1.80E-02"
                     /label="PKSI-KS_m4"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="988"
                     /protein_start="975"
                     /score="8.6"
                     /tool="antismash"
                     /translation="FSADADGTGWGEG"
     CDS_motif       complement(287..334)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0005"
                     /evalue="4.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="928"
                     /protein_start="912"
                     /score="21.5"
                     /tool="antismash"
                     /translation="GPALTVDTACSSSLVA"
     aSDomain        complement(884..1099)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HCC61_RS29980_ACP.1"
                     /evalue="1.10E-30"
                     /label="HCC61_RS29980_ACP.1"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="729"
                     /protein_start="657"
                     /score="97.7"
                     /tool="antismash"
                     /translation="SLVRDETASVLGHGDAGDLRVDRPFRELGFDSLRAVQLRNRLAAA
                     TGLTLSSTIVFDHPTADDLTAYLEAEL"
     aSModule        complement(884..1921)
                     /domains="nrpspksdomains_HCC61_RS29980_PKS_KR.1"
                     /domains="nrpspksdomains_HCC61_RS29980_ACP.1"
                     /incomplete
                     /locus_tags="HCC61_RS29980"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(1247..1291)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0004"
                     /evalue="7.00E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="608"
                     /protein_start="593"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="PMLAAPTAAASLLVA"
     aSDomain        complement(1415..1921)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HCC61_RS29980_PKS_KR.1"
                     /evalue="3.30E-49"
                     /label="HCC61_RS29980_PKS_KR.1"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="552"
                     /protein_start="383"
                     /score="159.2"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGRRVAHWLAAEGADRVVLVSRSGPEAPGAADLT
                     ALGVETAVVAADLTDPERARELMAEWRPTAVVHAAGVLDDGVVDSLTPDRFEAVVGPKA
                     DALENLRQAAADETVFVVFTSVAGVVGSPGQGNYAAANAACDALVASYRDQGLRAVSVA
                     WGPWAE"
     CDS_motif       complement(1469..1564)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0003"
                     /evalue="1.60E-14"
                     /label="PKSI-KR_m4"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="534"
                     /protein_start="502"
                     /score="46.4"
                     /tool="antismash"
                     /translation="FVVFTSVAGVVGSPGQGNYAAANAACDALVAS"
     CDS_motif       complement(1820..1918)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0002"
                     /evalue="9.30E-14"
                     /label="PKSI-KR_m1"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="417"
                     /protein_start="384"
                     /score="43.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGRRVAHWLAAEGADRVVLVSRS"
     CDS_motif       complement(2927..2962)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HCC61_RS29980_0001"
                     /evalue="1.90E-03"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="HCC61_RS29980"
                     /protein_end="48"
                     /protein_start="36"
                     /score="11.8"
                     /tool="antismash"
                     /translation="YWVEHVRVAVRF"
ORIGIN
        1 cattcgacgc accgtcctga ttcaccgcag aaccccgcac caccgccaac acccgatgcc
       61 cgttccgctc cgcctccgac aaccgctcca acaccaacca cccagcaccc tcaccccaac
      121 cagtgccatc ggcgtcggcg ctgaaggcgt ggcagcggcc atcgctcgac aggcctcgct
      181 ggcgagagaa ctccacgaag gtgtacggcg tcgccatcac cgtcgcgccg cccgcgagcg
      241 ccagcccgca ctcaccgttg cgcaacgact gcgccgccag atgcaacgcc accaacgacg
      301 acgaacacgc cgtatccacc gtcaacgcag gcccctgaag acccagcgcg taggcgacgc
      361 ggcctgacat cacgctggcg ctggcagcgg tcgcgccata gccggccgcc tcctcgccgg
      421 cggccagcac gcgcgagccg tagtcctgcc cgttgctgcc gacgaacacg ccgacgtcgc
      481 cgctccgcag cgaggcgggg gcgagaccgg cgttctcgaa cgcctcccag gcggtctcca
      541 gcagcagccg ctgctgcggg tcggtcacgg ccgcctcgcg cgggctgatg ccgaagaact
      601 cggcgtcgaa gtcgcccgcg tcatggagga atccgccttc gcgaacggtc gtcgtgcccg
      661 cccggtcggg atccgcgtcg tagatgtcgg acaggctcca gccgcggtcg tcggggaagg
      721 cgcccacggc gtccacgccc tcatccacca gccgccacag ctcatccggc gaacccacac
      781 caccggggaa ccggcaactc atcccgacaa tggcaaccgc ctcatccacc accgcacgcc
      841 cactgcccgc cgaagcggcc tccagcgtgt ccgtgtcgcc ggacagttcg gcctcgaggt
      901 acgcggtcag gtcgtcggcg gtcggatggt cgaagacgat cgtggaggag agcgtcagcc
      961 cggtggccgc ggcgagccgg ttgcgcagct ggaccgcgcg cagcgagtcg aagcccagtt
     1021 cgcggaacgg ccggtcgacg cgcaggtcgc cggcgtcccc gtggccgagc accgacgccg
     1081 tctcgtcccg taccagcgac agcacggctc gtcggcgctc cgcctccgac aggccggcga
     1141 agcggctcgc gccggcttcc acatcggcgg tacggctgac gcccggcagc tcgtcgaaca
     1201 gctgcgagga cgccaactgc cctgccagca gcggccagtc ggcgtcggcg accagcagcg
     1261 aagcggccgc cgtcggagcg gccaacatcg gggaagccgc cgacggtacg gccgtcagcg
     1321 gggcggccgc tcggcggagg acggagagcg cgaggtgcgg cggcatcggg gacaggccgt
     1381 cgaggccgag gcgcttcccc tcggccatcc cgtcctcggc ccacgggccc cacgccacgg
     1441 agaccgcccg cagtccctgg tcgcggtagc tggcgacgag cgcgtcacaa gcggcgttgg
     1501 cggcggcgta gttgccctgc cccggcgaac cgacgactcc ggccacggag gtgaacacga
     1561 cgaacacggt ctcgtccgcc gccgcctggc gcaggttctc cagcgcgtcg gccttcgggc
     1621 ccaccacggc ctcgaagcgg tccggtgtga gggagtcgac gacaccgtcg tcgagcactc
     1681 ccgcggcgtg gaccaccgcg gtcggacgcc actcggccat caactcccgc gcacgttccg
     1741 ggtcggtcag atccgcggcc accacagcgg tctccacacc gagcgcggtc aggtcggccg
     1801 caccgggtgc ctccgggccg ctcctgctga cgagcaccac acggtcggcc ccctcggcgg
     1861 cgagccagtg cgccacacga cggccgagcg cgccggtgcc cccggtgacc agcacggtgc
     1921 cggacggctc ccaaccgccc gccgcgccgg gcgcgttcac aagccggcgt acgtagctgc
     1981 cctcggaccg gaccgagacc tcgtgctcag gtccggcgac ggcctcgggc agccgcgccc
     2041 acgcgtcgtc accgatcagt tcgacgagcc cgccccaccg gccgggttcc tccgccgcta
     2101 cgacccggcc gagtccctgt gccagtgcct gcaagggttc gccggtcgtg acgcaccaca
     2161 gcggagcgtc gattccggcg tcgccgaggc cctgtaccaa ggccagcagc gtggccccgc
     2221 tccgccgggc gtcgccgagc gcgagcaggg agacgacccg gtcgggacgc agcccccgca
     2281 gccgttcgca cacctcggca cggtcgacgg tgtccagttc caccgtcgta ctgccctcgg
     2341 ggatcccggc gggcggccgg aacccaggcg gttgcaccac gagccatgtg ccggactcgg
     2401 cgggcgcggt gggctcggcg ggcgcggtag gctcggcggg cttgccggac ccggcggacc
     2461 gcccggccac ggggacccac gccacctggt ggcaccagcc gtccacggtg gacttgttct
     2521 cggtacggcg gcgcagctcc gccagcgcgg gcagcgccgg ctccagcgaa gtccggaggt
     2581 cgtccggcag gtcgtcgagg tcgagtccgt cccaccaggc cgtgtcggcg gacgtgtcct
     2641 ccagccagta acgccggtgc tggaacgggt aggtgggaag gtcgacgcgg cgggctgcgg
     2701 ggaacagctc cgaccagtcg acgtccgcac cgcgtacgga cagccgggcc agggcggtca
     2761 acagcgcggc gggttcgccg ggttcgtgct cgccctggct gagcgcgggt atgaacgagc
     2821 cgtcgggtgg gaggagttgt tgtcccatgc cgctcagtac ggcatccggg cccagctcca
     2881 gcaccgtcga cacaccctcc gccgccagcg cttggacggt gtcggcgaac cgtacggcca
     2941 cccgcacatg ctccacccaa tactccgggc ccagcgcctc cacccgctca ccggaaacca
     3001 tcgacaccaa cggaatacgc ggcgagtgga actccaaccc ggcgacgaca cccgcgaatt
     3061 catcgagcat cggctccatc aacggcgaat
//
