LOCUS       NZ_JAASAG010000019     11116 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. HNM0575 scfid19, whole genome shotgun sequence.
ACCESSION   NZ_JAASAG010000019
VERSION     NZ_JAASAG010000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. HNM0575
  ORGANISM  Streptomyces sp. HNM0575
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 70239)
  AUTHORS   Xiao,K.
  TITLE     New actinomycetes from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 70239)
  AUTHORS   Xiao,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) College of Tropical Crops Hainan University,
            Hainan University, Renmin Avenue, HaiKou, HaiNan 570100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2018
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012726265.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 05:29:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,992
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 69
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 15 of 126
            Pseudo Genes (incomplete)         :: 115 of 126
            Pseudo Genes (internal stop)      :: 5 of 126
            Pseudo Genes (multiple problems)  :: 8 of 126
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 38093
            Orig. end                         :: 49209
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASAG010000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11116
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11116
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11116
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6116](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6116
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            584..940
                     /locus_tag="HCC61_RS29065"
                     /old_locus_tag="HCC61_29060"
     CDS             584..940
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548475.1"
                     /locus_tag="HCC61_RS29065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29060"
                     /product="arsenate reductase family protein"
                     /protein_id="WP_169140679.1"
                     /transl_table=11
                     /translation="MEIWINPACSKCRSALSVLDEEGAEYTVRRYLDDPPAPAELVDVL
                     RRLGLEPWDIARTGEPAAAELGIASWPRDPESRDRWIDALAAHPSLIQRPIITADDGSA
                     VVGRSPESVRSVLE"
     misc_feature    complement(587..600)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(953..1429)
                     /locus_tag="HCC61_RS29070"
                     /old_locus_tag="HCC61_29065"
     CDS             complement(953..1429)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383220.1"
                     /locus_tag="HCC61_RS29070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29065"
                     /product="DoxX family protein"
                     /protein_id="WP_169140681.1"
                     /transl_table=11
                     /translation="MNQSADTPSGTPAEGPAASGPVARQGRGKEIALRVLQVVVGLFYA
                     LGSAVPKLIGQQMAVDTFQDIGAGMWLMYLVGVLELAGGIGLITPWLSGLAALGLIALM
                     IGAFITQLTVFHGDSAAFPLVMIVPLAVIAWGRRSTLKALPTLLHGKPGARPAP"
     gene            complement(1590..2060)
                     /locus_tag="HCC61_RS29075"
                     /old_locus_tag="HCC61_29070"
     CDS             complement(1590..2060)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /locus_tag="HCC61_RS29075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29070"
                     /product="DoxX family protein"
                     /protein_id="WP_169140683.1"
                     /transl_table=11
                     /translation="MANASGIPPTSQAGPSAARPSRPQGPARIALEVALWAVQLYLAYF
                     MITVGALPALTADSGAKGTFQDIGFGLWFMYLTGVLELLGAVGLITPWFSGLAALGLMF
                     VMSGACLTHLTLYDGRGVSTPALILIPLALVVIGRWSTVRKLGAWLTGGHRN"
     misc_feature    complement(2338..2351)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.89"
                     /tool="antismash"
     gene            complement(2356..3030)
                     /locus_tag="HCC61_RS29080"
                     /old_locus_tag="HCC61_29075"
     CDS             complement(2356..3030)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041196.1"
                     /locus_tag="HCC61_RS29080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29075"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_169140685.1"
                     /transl_table=11
                     /translation="MRQERAELTRQALTMAAAKSFDRLGYQRTTLFEVSRGAAMSKGAL
                     SFHFGSKEELADTVRREACTRSYRAAELLRDKAQPALQTLVDLTHLVAHQLEHDHLARA
                     AVKLARELDTPPDPEVNPFACWQQVFTDVAREAEKEGTLRGEATTEAVAELAFCLASGA
                     HSEQTSRTGDSGADVHQRLAGHWQLLLHGLVTQPGPSGLRSAGTEADWLRTVERQRIRE
                     AD"
     misc_feature    complement(3127..3147)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     misc_feature    3143..3152
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     misc_feature    complement(3164..3176)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.71"
                     /tool="antismash"
     gene            complement(3324..4277)
                     /locus_tag="HCC61_RS29085"
                     /old_locus_tag="HCC61_29080"
     CDS             complement(3324..4277)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="HCC61_RS29085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29080"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_169140687.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3e-13, bitscore:
                     41.2, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTARVVLTGASGFIGSAVLRRLTGQDADVRVLARRDLPLPAGVEQ
                     VHADLARPGTLTGACSGARVLVHAASHIGSDEQLCTRVNDLGTDALMREAERAGVERIV
                     SLSTTAVYGRGPHRGAAVGDLEPAPGSPASRSRLAGEAHALRHGALVLRTGLVTGAGDR
                     WVVPALAELVRRVPGHWRGGRAELSMIDVNDLARLVTTAALATTGPSGSGDPARGVRHA
                     VHPEPVRNRDLLGRLAQLGVLPPVRGELTWRECQDLLEAHPGRVGERQLRLLAFDHHYA
                     TDVWQACRSAPGEGPLAALGEAAPWYRAHLARTASS"
     gene            complement(4274..4915)
                     /locus_tag="HCC61_RS29090"
                     /old_locus_tag="HCC61_29085"
     CDS             complement(4274..4915)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628787.1"
                     /locus_tag="HCC61_RS29090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29085"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_240969390.1"
                     /transl_table=11
                     /translation="MAAARAKPMQQRAVETRQLILEAAAEVFDEGGYKAARITEIMQRA
                     GITQGALYFHFSSKLDLARTVMHAQRDAVVLPPGPDGLQRLIDTTLLLARELQTNKLLR
                     AGVRLAVEQEEIGFRDDEPYRQWAEVFRGQLAAAESRGQLRNGVEVERLAVLLVGCYTG
                     VQHFSKLSCRREDLPDRIADLWQYMLLGIAPPQVAAELVVNPETPLLAAG"
     gene            5001..6116
                     /locus_tag="HCC61_RS29095"
                     /old_locus_tag="HCC61_29090"
     CDS             5001..6116
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="HCC61_RS29095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29090"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_169140689.1"
                     /sec_met_domain="AfsA (E-value: 1e-22, bitscore: 71.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFTPDVPQLPENTAYSWPVPRELVHKTAHTEVLLTGWRQSGERE
                     FEVGAQWPRDHGFWRTDDGGVQDPMLLVETTRQALPLFAHAAFGVPAGHQLIWDHQHCV
                     FSVPFLAVSGSPAEVTLRLSASRPPEHGARKRQIALTMSVHCGERTLGTVSTVYSAHSP
                     AVYRRLRGPGTPAPPEAMAAALVPPRPLAPALVGRESEREVVLAAADDGDGDGDGTAVA
                     GRAGRVVNGRGGEGSWPRRQLRVLTSSPWLFDHPVDHAPGMLLMEAARQVSHLAAAPHR
                     PRLTRMDADFHRYVDLDEPGWVDVSPGPVAPAAGAPQPSGERTVTVEITQHGEKAFSAV
                     VGFVTEETPDAAGPAGRKGDTAPAAVPPGLG"
     gene            6291..7181
                     /locus_tag="HCC61_RS29100"
                     /old_locus_tag="HCC61_29095"
     CDS             6291..7181
                     /GO_function="GO:0008276 - protein methyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518540.1"
                     /locus_tag="HCC61_RS29100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29095"
                     /product="HemK family protein methyltransferase"
                     /protein_id="WP_169140691.1"
                     /transl_table=11
                     /translation="MSVPPPSDPPWVSCSALAARLREAGCVFAEDEAELMLSAAGTAAE
                     LAAMAERRATGLPLEHVLGRAEFCGMRMVVEPGVFVPRRRTEFLVRQAAAVTRPGSLVV
                     DLCCGSGALGAALAAAVAREHPPHEQGSGAAGAAGAAGAAGAVELHAADIDPAAVRCAR
                     RNVAAAGGRVHEGDLFAALPAVLRGRVDVLLANVPYVPTDSLGLLPAEARDHEPVIALD
                     GGADGLDVLRRVAAEVRHWLAPGGRLLVETSEDQVEAAARVLERGGLTVRTARREEPAA
                     TVVTGRRNASRDGRT"
     gene            complement(<7286..7681)
                     /locus_tag="HCC61_RS29105"
                     /old_locus_tag="HCC61_29100"
                     /pseudo=""
     CDS             complement(<7286..7681)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003766.1"
                     /locus_tag="HCC61_RS29105"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29100"
                     /product="cytosine permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="METNGLDIIGGTERKGRPAQLFWPWFASNVSVLGLSYGSFALGFG
                     IGFWQALAAGIAGIVFSFLLCGLVAVAGKRGSAPTMVLSRAAFGVRGNRLPSAVSWVLT
                     VGWETVLVILATLATATVFDRLGWGGGT"
     gene            8292..8639
                     /locus_tag="HCC61_RS29110"
                     /old_locus_tag="HCC61_29105"
     CDS             8292..8639
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HCC61_RS29110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HCC61_29105"
                     /product="hypothetical protein"
                     /protein_id="WP_169140693.1"
                     /transl_table=11
                     /translation="MGTATIQVRVLAVAGRRVWMAVSGTLSGATLRVLHYSLSARATDE
                     RTQIYLDLNAVRWDSSLSAAELLRLLPAGARLRFHIVGAHEDFRERLAADARCRLHSDV
                     ASAWATWSRQS"
     gene            complement(8779..9189)
                     /locus_tag="HCC61_RS29115"
                     /old_locus_tag="HCC61_29110"
     CDS             complement(8779..9189)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430555.1"
                     /locus_tag="HCC61_RS29115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29110"
                     /product="ATP-binding protein"
                     /protein_id="WP_346777436.1"
                     /transl_table=11
                     /translation="MSPRRPRHIARVEYTLPRRAASARWARQLTTGFLTGSHTPPEAAH
                     RVDEARLIVSELVTNAALHGQGRCRLRLSSGEGTVTVEVRDEGRRLPRVRRPARESESG
                     RGIAMVSSLARRMDVTAAPGGGKTVSAVLDAA"
     misc_feature    9336..9351
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.78"
                     /tool="antismash"
     gene            complement(9374..9748)
                     /gene="mnhG"
                     /locus_tag="HCC61_RS29120"
                     /old_locus_tag="HCC61_29115"
     CDS             complement(9374..9748)
                     /GO_function="GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mnhG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358520.1"
                     /locus_tag="HCC61_RS29120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29115"
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="WP_169140697.1"
                     /transl_table=11
                     /translation="MNWSGAADIAAMACMMAGAVLCLLAGIGLVRLPDVLTRMHAATKP
                     QVLGLLFVLVGAGLRLRSAADLGTLLLVGIFQLLTVPVAAHMVGRAAYRTGRTDREHLV
                     VDELAAAAEKSDKSPGGHSR"
     gene            complement(9745..10134)
                     /locus_tag="HCC61_RS29125"
                     /old_locus_tag="HCC61_29120"
     CDS             complement(9745..10134)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907071.1"
                     /locus_tag="HCC61_RS29125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29120"
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="WP_346777437.1"
                     /transl_table=11
                     /translation="MDTALSVVVGMLLASGALVTVRLVRGPSTLDRAVALDALAAVVMA
                     GIGVLTAARQIPYYLPALLVLSFLGFTGSVSIARFLALRDEAGSEDPDEGGGGGERAAA
                     GHDGPHGGRNGKRADGGAAEQGSGG"
     gene            complement(10119..10772)
                     /locus_tag="HCC61_RS29130"
                     /old_locus_tag="HCC61_29125"
     CDS             complement(10119..10772)
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468392.1"
                     /locus_tag="HCC61_RS29130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_29125"
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="WP_169140699.1"
                     /transl_table=11
                     /translation="MSEQSAAGPGTPDPAGGVPGGPPRGRSRIRAAQWPTVLGLTVLWV
                     LLWGTLSAANVLTGVLVGVVVCVIFPLPPIETQVRLRPVGVLRFTVRFVLDMAASSWRL
                     NRYILGTRPPVCAVLAVRMRSPSDLMLTATSVAVAAVPGSNVLDVHRASGTMYVHVMGA
                     EGETERERARYEVLRLEDRVVRAFGTRADVAALDARRGGVTGGSGGGSGPWTRP"
ORIGIN
        1 gcatcgggct catcggggac gacgagcgga tcttctccgt cgccgagcgc gtcagcggcc
       61 accgcgacgc gctgcgcgcc gggaacctgc cctacgaacc ggagtcgacg cgcctgggcg
      121 cgcgcaccgc cgaggaggcc atgcaggcgt gccgggagct cctcggctcc tcccggccgc
      181 ccacggcgct cttcacgctc aacaaccagt gcaccatcgg ggccgtgcgc gcgctgcgcg
      241 ccctcggtct gagcaggaag gtggcgctgg tcggcttcga cgacttcgag acggccgagc
      301 tgatcgaccc gccggtcacg gtgatctccc acgacgtcga ggagatcggg cggaccgccg
      361 cccggctgat gttcgagcgg atggcgggcc actcccccgc accggccagg acgacgctgc
      421 cgacgaagct gatcacccgc ggctcgggtg agattccggg gcctcaggcc gcggcgggaa
      481 gcggcccacg gtgaacggcc gcgcccggcc ccaccccgtt cgcggctgtt ctcagtgacc
      541 cccggcggcc ggcgcccggc cgccgaaccg aaaggccgag agcgtggaga tctggatcaa
      601 ccccgcctgt tccaagtgcc gttcggcact gtcggtgctg gacgaggaag gcgcggaata
      661 caccgtccgc cgctacctcg acgacccgcc ggctccggct gaactggtcg acgtactgcg
      721 gcggttgggg ctggagccgt gggacatcgc ccgcaccggc gagcccgccg ccgccgaact
      781 cggcatcgcc tcctggcccc gtgacccgga gagccgcgac cggtggatcg acgcgctcgc
      841 ggcccacccc tccctgatcc agcggccgat catcaccgcg gacgacggga gtgccgtggt
      901 gggccgctcg ccggaatccg tcaggtccgt actggaatga ggagcccgct gctcaagggg
      961 cgggccgggc acccggcttg ccgtgcagca gcgtgggcag agccttcagc gtgctccggc
     1021 gtccccatgc gatgacggcg agcgggacga tcatcaccag cgggaaggcg gccgagtcgc
     1081 cgtggaagac ggtgagctgc gtgatgaacg caccgatcat caaggcgatc agccccaggg
     1141 cggccagacc cgacagccac ggcgtgatca gccctattcc gccggcgagt tcgaggacgc
     1201 cgacgagata catcagccac atgccggcgc cgatgtcctg gaaggtgtcc accgccatct
     1261 gctggccgat cagcttggga accgcgctgc cgagcgcgta gaacaggccg accaccacct
     1321 gtagtacgcg cagggcgatc tccttgcccc gtccctgccg ggccaccggt cccgatgcgg
     1381 ccggcccctc ggccggggtg cccgacgggg tgtctgcgga ctggttcatg acgttctcct
     1441 ctcgggtgtg cactttccat accccgggag cagacccgtc gcgcacgggt ttctcatcgc
     1501 gacggctcgc aggggcccgt cgaagaggcg ccggacgccg ccgtcccggc caaggcggcc
     1561 aaagggggcg gaatccggcg tctggcaggt cagttccggt gtccgcccgt gagccacgcg
     1621 ccgagcttcc tcaccgtcga ccagcggccg atgacgacga gcgcgagcgg gatgaggatc
     1681 agcgccgggg tggagactcc gcggccgtcg tagagggtga ggtgcgtcag gcacgcgccc
     1741 gacatcacga acatcagccc gagcgcggcg agaccggaga accagggcgt gatcaggccg
     1801 acggcgccca gcagttcgag cacgccggtg aggtacatga accacaggcc gaagccgatg
     1861 tcctggaagg tccccttggc gccggagtcg gcggtcagcg caggcagcgc cccgaccgtg
     1921 atcatgaagt acgcgaggta caactgcacg gcccacaggg cgacttcgag cgcgatacgg
     1981 gccggtcctt gagggcggga gggacgtgcg gccgagggac cggcctggga ggtcggcggg
     2041 atgccgctcg cgttcgccat gatggactcc ttctgcggac ggtgcagacg cgattgccgg
     2101 ggcggcccgg cagctgacgt ctaccaccgg cacggcctcg gcggaactcc cccgccacgg
     2161 cagaagcgag ggcgccgccc ccgcgtggaa cgcggggcct gtgagatccg tatcacgtgc
     2221 ccggtacgca gcgcagacag ttgtccatca tggtcgacat ccggtccgcc agcctcccgt
     2281 acggcgggcg acggccgccc accgcacggc acccggtggc cctgcgaacg gtgttatgcg
     2341 cgtagcgtga cgggctcagt ccgcctcgcg gatccgctgt cgttccaccg tccgcagcca
     2401 gtccgcctcg gtgccggcgc tccgcaggcc ggacggtccg ggctgcgtca cgaggccgtg
     2461 cagcagcaac tgccagtgcc ctgcgaggcg ttggtgcaca tcggcgcccg agtcgcccgt
     2521 gcggctggtc tgttcggagt gggctccgga ggcgaggcag aaggcgagtt cggcgacggc
     2581 ctccgtggtg gcctcgccgc gcagcgtgcc ctccttctcc gcttcgcggg ccacgtcggt
     2641 gaagacctgc tgccagcagg cgaacgggtt cacctccggg tctggcgggg tgtccagttc
     2701 gcgtgcgagc ttcaccgcgg ccctcgccag atggtcgtgt tccagctggt gggccacgag
     2761 gtgagtcagg tcgacgaggg tctggagtgc gggctgcgcc ttgtcccgga gcagttcggc
     2821 ggcacggtag gaacgggtgc aggcctcgcg gcgtacggtg tcggcgagct cttccttgct
     2881 tccgaagtgg aaggagagag cgcccttgct catcgctgcg ccgcggctga cctcgaacag
     2941 cgtggtgcgc tggtagccga ggcggtcgaa cgacttcgcc gccgccatcg tcagtgcctg
     3001 ccgggtgagt tctgcgcgct cttgcctcat tcggctttct ttcccttcaa tgcgttccgg
     3061 tccgcgggga gacggaccgt caggggcggc agtgctgtgg ggagtcgaga aactgccgcc
     3121 tccacaaaca tacaaagtgt tctgtttttt tgcgccggaa ggggtgacag agtctcctga
     3181 cagcaggcgt cccggggtgc tgaagaggtg ccggcggtgc gggtggtgcc gggccgtacg
     3241 gcggacttca gggccgtagg gcggacttcg ggccgtacgt caggagtcag gggggcaggt
     3301 cagggggtcc aggggtccga aggtcaggac gaggccgtac gcgcgagatg ggcgcggtac
     3361 cagggggcgg cctcgccgag cgcggccaac gggccctcgc cgggggccga ccggcaggcc
     3421 tgccacacgt ccgtggcgta gtggtggtcg aaggccagca gcctcagctg gcgttcgccg
     3481 acgcggccgg gatgcgcctc cagcagatcc tggcactccc gccacgtcaa ctccccgcgg
     3541 acgggcggca gtacgccgag ctgtgcgagc cttcccagca ggtcccggtt gcgtacgggc
     3601 tcggggtgca cggcgtgccg gaccccgcgg gccggatcac cggagccgga cgggcctgtc
     3661 gtcgcgagcg cggcggtggt gacgagccgc gccagatcgt tcacgtcgat catggacagc
     3721 tccgcccgcc ctccgcgcca gtgacccggt acgcggcgca cgagttcggc cagagcgggg
     3781 acgacccagc ggtcccccgc cccggtgacc aggccggtcc gcagtacgag ggcgccgtgc
     3841 cgcagggcgt gcgcctcgcc ggcgagtctg ctgcggctgg cgggcgagcc cggagcgggt
     3901 tccaggtcgc cgacagcggc gccgcggtgg ggtccgcgtc cgtagacggc ggtggtggac
     3961 agcgacacga tgcgctccac gccggcccgc tcggcttcgc gcatcagcgc gtcggtgccc
     4021 aagtcgttga cccgggtgca cagttgctcg tcggagccga tgtgggaggc ggcgtgcacc
     4081 agcacgcgcg caccggagca ggccccggtg agggttccgg gccgggcgag gtcggcgtgg
     4141 acctgctcga ccccggcggg cagcgggagg tcccggcggg cgaggacccg tacgtccgcg
     4201 tcctgccccg tcaggcggcg caggaccgcc gagcctatga agccgctcgc gccggtgagc
     4261 accacccttg cggtcatccg gccgccagca gcggggtctc cgggttgacg acgagttccg
     4321 cggccacctg cggcggtgcg atccccagca gcatgtactg ccacaggtcc gcgatgcggt
     4381 cgggcaagtc ctcgcgccgg caggagagtt tggagaagtg ctgcacaccg gtgtagcagc
     4441 cgaccaagag caccgcgagt cgctcgacct cgactccgtt gcgcagctgg cctcttgact
     4501 ccgcggcggc gagctggccg cggaagacct ccgcccactg ccggtacggc tcgtcgtcgc
     4561 ggaagcctat ctcctcctgc tccacggcga gccgcacccc cgcgcgcaga agcttgttgg
     4621 tctggagctc gcgggcgagg aggagggtcg tgtcgatgag gcgctgcaac ccgtccggcc
     4681 ccggcggcag tacgaccgcg tcgcgctggg cgtgcatgac cgtacgggcc aggtccagct
     4741 tcgaggagaa gtggaagtag agggctccct gggtgatgcc cgcccgttgc atgatctccg
     4801 tgatccgtgc cgccttgtag cccccctcat cgaaaacctc agccgccgct tccaggatca
     4861 actggcgtgt ctccacggca cgttgctgca tcggtttcgc tctcgccgca gccatccgat
     4921 ctcgtcccca gtggttctat cgtctttcag gcacaggtct ctgtcatttt ctcggaccgc
     4981 acccgcccgg aggaccgcac atggctttca cccccgatgt gccccaactg ccggagaaca
     5041 cagcgtactc gtggcccgtg ccgcgggagc tggtgcacaa gaccgcgcac acggaggtgc
     5101 tgctgaccgg ctggcggcag tccggggagc gggagttcga ggtcggtgcg cagtggcccc
     5161 gggaccacgg tttctggcgt acggacgacg gaggcgtaca ggatccgatg ctgctggtcg
     5221 agacgacccg gcaggcgctt ccgctgttcg cgcacgcggc gttcggggtt ccggccgggc
     5281 atcagctcat atgggaccac cagcactgcg tcttctcggt gcccttcttg gcggtgtcgg
     5341 gcagcccggc cgaggtgacg ctgcggctgt cggcgtcccg gccccccgag cacggggccc
     5401 ggaagcgcca gatcgcgctg acgatgagcg tgcactgcgg ggagcgcacc ctcggcaccg
     5461 tgtccaccgt ctacagcgcg cacagccccg cggtgtaccg gcggcttcgc ggcccgggca
     5521 cgcccgcgcc gccggaggcc atggccgccg ctctcgtgcc gccgcgtccg ctggccccgg
     5581 cgctggtcgg gcgcgagagc gaacgggagg tggtgctggc cgcggccgac gacggggacg
     5641 gggacgggga cggcacggcc gtggcgggga gagccgggcg ggtcgtcaac gggcgtggcg
     5701 gggaggggag ttggcctcgc cggcagctgc gggtgctcac gtcgagtccg tggctcttcg
     5761 accaccccgt cgaccacgca cccggcatgc tgctgatgga ggccgcccgg caggtctcgc
     5821 acctcgccgc agctccgcac cggccgcggc tgacgcgcat ggacgcggac ttccaccgct
     5881 atgtcgacct ggacgagccc ggttgggtcg acgtctcccc cggtcccgtg gctccggcgg
     5941 cgggagcgcc gcagccgagc ggggagcgca ccgtgaccgt ggagatcaca cagcacggcg
     6001 agaaggcgtt cagcgcggtg gtcggcttcg tcacagagga gacgccggac gccgccggac
     6061 cggccgggag gaagggagac acggcgcccg ccgccgtccc gccgggcctc gggtagggtt
     6121 gcctccctca gcacacacgc cgtccgagga ggtgggagcc atcaccaccg tggaagttcc
     6181 cctcccgcgc ggcgaggtcc gcgccgctga cgctccgtcc cgctgaagcg ccgacgcagc
     6241 ccgcacggga gccgggagcc cggtccggac ccctgagagg cacgcgccgt atgtctgtcc
     6301 ctcccccatc cgatcccccg tgggtctcgt gctcggcact ggccgcccgc cttcgtgagg
     6361 cgggctgcgt attcgccgag gacgaggccg agttgatgct gtccgccgcc ggtacggccg
     6421 ctgagctggc cgccatggcc gagcgccggg ccacggggct ccccctcgaa cacgtcctgg
     6481 gccgggcgga gttctgcggc atgcggatgg tggtggagcc gggggtgttc gtgccgcgca
     6541 ggcgcaccga gttcctcgta cggcaggccg cggccgtcac ccgtcccgga agcctggtgg
     6601 tcgatctgtg ctgcggttcg ggcgcgctgg gcgccgcgct cgcggctgcg gtggcacgtg
     6661 agcacccgcc gcacgagcag ggcagcggcg cggcgggtgc cgccggtgcc gccggagccg
     6721 ccggtgcggt cgaactgcac gccgccgata tcgatccggc agccgtgcgg tgtgcacgcc
     6781 gcaacgtcgc cgccgccggc ggccgggtcc acgaaggcga cctgttcgcg gcgcttccgg
     6841 cggtgctgag gggccgcgtc gacgtgctgc tcgccaacgt gccgtatgtg cccacggatt
     6901 cgctggggct gctgcctgcg gaggcccgcg accacgagcc ggtgatcgcg ctggacggcg
     6961 gtgcggacgg gctcgatgtg ctgcgccggg tggccgccga ggtgcggcac tggctcgcac
     7021 cgggtggccg gctgctggtg gagacgagcg aggaccaagt cgaggcggcc gcaagggtgt
     7081 tggagcgcgg cgggctcacg gtgcggacgg cccgccgcga ggaaccggcc gccaccgtcg
     7141 tcaccggacg ccgcaacgcc tcccgcgacg ggcggacttg accggttgat cgtctgagcc
     7201 cggcaccggt cggccgccgg gccccgatcg ctcagccgcc ctcgtccgta ccggcttccg
     7261 tgcggctggc ggagacaccg gggccggtgc ctccgcccca gcccagccgg tcgaagaccg
     7321 tcgccgtggc gagcgtggcg aggatgacca gcacggtctc ccagccgacc gtcagcaccc
     7381 aggagacggc ggagggcagc ctgttgccgc gcactccgaa cgccgcccgg ctgagcacca
     7441 tcgtcggcgc ggatccgcgc ttccccgcca ccgcgacgag gccgcagagc aggaaggaga
     7501 agacgatgcc cgcgatgccg gcggccaggg cctgccagaa gccgatgccg aatccgagcg
     7561 cgaaggagcc gtaactcagg cccagcaccg agacgttgga ggcgaaccac ggccagaaca
     7621 gctgcgcggg ccgtcctttg cgctcggtgc cgccgatgat gtcgaggccg ttggtctcca
     7681 cggcgagcgc ggccttgcgg gcgggccgct cggcggcggc gtgcccgccg ggtgcgtgcg
     7741 gcggctgcgg cgagtcctgt gatcccgcct ccgtaccggc cacttggcac ccccgtccgg
     7801 cctgatccga cgcttggagc ctgctggcgg gtgcggtgcc gcgtcaagta cgcaccgtcg
     7861 gggcgctgtt ggggctgtcg ggcattccgg ccgcgagcac ggcgctgctg gacacggctc
     7921 tgccgggagg cccggacccc gggcctccgg gcctccgcgg ggcgcaccgc cccgtgacgt
     7981 gcgaagcaac cgcgttctgc catgaccgtc gagggcgcct ccggagtcgc ggcgtaccgc
     8041 cgagcaggtt ccggagcccc gcgcggcacc gcggcccggg aagaccagac aaccggaacg
     8101 agggcgttct cgtgaggaat tgagggggga acgtgccgtt cgttcacccg cctccccgaa
     8161 cgtcgacccg gccgtgatac ttgtgtgcgt cggccaggga acggcagtca gaacgatcga
     8221 tctgcgcggt ccgcagcagt acgggcgttg cccgtgccgc gcgcgaacgc gcagtctgga
     8281 caagggcttt catggggacg gcgacgatac aggtgcgagt cctggccgtc gcgggccggc
     8341 gcgtgtggat ggcggtgagc ggcacgctgt ccggcgccac gctcagagtc ctccactaca
     8401 gcctgagcgc ccgcgccacg gacgagcgga cgcagatcta cctcgacctc aacgcggtgc
     8461 gctgggacag ctccctttcc gcagccgaac tcctccggct gctgccggca ggcgcccggc
     8521 tgcgcttcca catcgtcggc gcgcacgagg acttcaggga gcggctggct gcggacgcgc
     8581 ggtgccgtct gcactcggac gtggcctccg cgtgggccac gtggtcgcgg cagtcctgaa
     8641 ctcccgccgc cgcggcagga gttggccatc cggccccccg gccgtcaccg gaacggttcc
     8701 gcggcggact tacggagcgg aggtccgccg gggccagagg ccttccgagc cagcgggcct
     8761 gcggcccggc gctcccctct aagccgcgtc cagaacggcg ctgaccgtct tgcccccgcc
     8821 gggcgcggcg gtgacgtcca tccggcgggc gaggctgctg accatcgcga tgcccctgcc
     8881 gctctcgctc tcgcgggcgg gccgccgcac acgcggcaac cggcggccct cgtcgcgtac
     8941 ttcgaccgtg acggtgccct cgccgctgct cagccgcagc cggcaacgtc cctgaccgtg
     9001 cagcgccgcg ttggtgacga gctcggagac gatcagcctc gcctcgtcga cgcggtgcgc
     9061 cgcctccgga ggcgtatggc tgccggtgag gaatccggtg gtcagctggc gggcccagcg
     9121 ggccgacgcg gccctgcgcg gcagtgtgta ctcgacgcgt gcgatgtgcc gcggccggcg
     9181 cggactcacg cgcatgacgt tcacttcccc tcggtgccgg cgtacgcccg gggctgccgg
     9241 ggccggctgc gcgaatcgag acctgcggac tgtctttccc ggcgggagcg cgggcattca
     9301 ccgcgcggcg cgaccgggac atcccgggcg aaagcttcac ttttccttga cacacggagg
     9361 ccgcggccgt gcctcaacgg ctgtgcccgc ccggagactt gtcggacttc tccgctgccg
     9421 cggcgagttc gtcgacgacg agatgctcgc ggtccgtcct gcccgtgcgg tacgcggccc
     9481 gcccgaccat gtgcgccgcc acgggcacgg tcagcagctg gaagatcccg acgaggagca
     9541 gcgtcccaag gtcggcggcg ctgcgcagtc tcaggcccgc cccgacgaga acgaacagca
     9601 ggccgagcac ctgcggcttg gtggcggcgt gcatccgcgt cagcacgtcc ggcagccgta
     9661 cgagcccgat ccccgcgagc aggcacagca cggcgcccgc catcatgcac gccatggccg
     9721 cgatgtccgc ggcccccgac cagttcatcc gccgctcccc tgctccgccg cgccgccgtc
     9781 ggcgcgcttc ccgttgcggc cgccgtgcgg gccgtcatga ccggcggcgg cccgctcgcc
     9841 gcctccgccg ccctcgtccg ggtcctcgct tcccgcctcg tcccgcagcg cgaggaagcg
     9901 ggcgatgctc accgagccgg tgaagccgag gaacgacagg accagcagcg ccggcaggta
     9961 gtacgggatc tgccgtgccg cggtgaggac gccgatgccc gccatcacca cggcggccag
    10021 ggcgtcgagg gccacggcac ggtcgagggt cgaaggcccg cgtaccagcc gtacggtgac
    10081 gagcgccccg gaggcgagca gcatccccac gacgacgctc agggccgtgt ccacgggccg
    10141 ctccctccgc cggaaccgcc cgtgaccccg ccgcggcgcg cgtcgagggc cgcgacgtcg
    10201 gcgcgggtgc cgaaggcccg caccacgcgg tcctccaggc gcagcacctc ataccgcgcc
    10261 cgctcgcgct cggtctctcc ctccgcgccc atcacgtgca cgtacatcgt tcccgaagcc
    10321 ctgtgcacgt cgaggacgtt gctgccgggg acggccgcga ccgcgacgga cgtggccgtc
    10381 agcatcaggt cgctggggct gcgcatccgt accgccagca ccgcgcacac cggaggccgc
    10441 gtgccgagga tgtatctgtt gagacgccag ctcgacgcgg ccatgtcgag cacgaaccgg
    10501 accgtgaagc gcaggacgcc gacgggacgc agccgtacct gggtctcgat cgggggcagc
    10561 gggaagatca cgcacaccac gacgccgacg agtacgcccg tgaggacgtt ggcggcgctc
    10621 agcgtgcccc acagcagcac ccacagcacg gtcagcccga gcaccgtcgg ccactgcgcg
    10681 gcccggatcc ggctgcggcc gcgagggggt ccgcccggca ctccgcccgc ggggtccggc
    10741 gtgcccggcc cggcggcgga ctgttcactc atcggccgat caccgccttc acatacgggg
    10801 tgcgggcggt catctcggcg gcgacgcggc tcgtcaggga cgacagcggc cccgccagca
    10861 cggtgaccgc cagcccgaag gcgaccagcg cggcggtcgg tacgagcatc gccgccgggg
    10921 gctgtccggt ggtgatggca cgtcctccga agctcgcggc ctcggcccgg cccgcgacga
    10981 cgtccacgtc gacggctccg gtgccgccgc cgagggccgc cccaccgccc gcttcgcccg
    11041 ccgctccacc gcccgatccg ccgcccgccg actcctgcgg aagttcctct tccgtctcgt
    11101 cggtctcgac ggtgtc
//
