LOCUS       NZ_JAASAG010000007     10405 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. HNM0575 scfid7, whole genome shotgun sequence.
ACCESSION   NZ_JAASAG010000007
VERSION     NZ_JAASAG010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. HNM0575
  ORGANISM  Streptomyces sp. HNM0575
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 443037)
  AUTHORS   Xiao,K.
  TITLE     New actinomycetes from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 443037)
  AUTHORS   Xiao,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) College of Tropical Crops Hainan University,
            Hainan University, Renmin Avenue, HaiKou, HaiNan 570100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2018
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012726265.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 05:29:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,992
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 69
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 15 of 126
            Pseudo Genes (incomplete)         :: 115 of 126
            Pseudo Genes (internal stop)      :: 5 of 126
            Pseudo Genes (multiple problems)  :: 8 of 126
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 367884
            Orig. end                         :: 378289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASAG010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            282..878
                     /locus_tag="HCC61_RS19050"
                     /old_locus_tag="HCC61_19045"
     CDS             282..878
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055058.1"
                     /locus_tag="HCC61_RS19050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19045"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_169136826.1"
                     /transl_table=11
                     /translation="MARISLDPPRSAMLRIAAWYSRRKYGKVMDPLRAAGHNPKVLGAV
                     SLYESRVAKWRALDPGLKHIAELVTASRIGCSWCVDFGYWLADDLGMPMEKIRYVPAWR
                     EHRERFTELELLVMEFAEAMTETEPAVTDGMTRELIGRLGEPAFVELTAIVAVENHRSR
                     INAALGLVGQGFSDQCAVRPVEPAGKGGASGPADS"
     gene            complement(916..1311)
                     /locus_tag="HCC61_RS19055"
                     /old_locus_tag="HCC61_19050"
     CDS             complement(916..1311)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356213.1"
                     /locus_tag="HCC61_RS19055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19050"
                     /product="DUF952 domain-containing protein"
                     /protein_id="WP_169136828.1"
                     /transl_table=11
                     /translation="MAELLHLTERSLWEAARESGTYEMSTRGHTLAEVGFIHCSQRHQL
                     PPVARMLYGGWDPAELVLLVIDSERLSVPVRYEAPEPAAACADDGGSGTPDGGAVEEFP
                     HIYGPLPVSAVVAEEAWSWDTPVSGGS"
     gene            complement(1368..2138)
                     /locus_tag="HCC61_RS19060"
                     /old_locus_tag="HCC61_19055"
     CDS             complement(1368..2138)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="HCC61_RS19060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19055"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_169136830.1"
                     /transl_table=11
                     /translation="MSAGETSASQVKSAVRTVELLEFFAGRPGMHSLADVQEAVGYPKS
                     SLYMLLRTLVDLGWVETDATGTRYGIGVRALLVGTSYIDGDEAVAAARPTLDRLSDDTT
                     ETIHMARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYDDEQVRKML
                     PETLSSLTENTLTDREALIEELALVREQGYAVDREENTLGLRCFGVAIPYRTPARDAIS
                     CSVPVARLTPAHEQTIKDALFDARDRLTLATRRL"
     gene            2260..3792
                     /locus_tag="HCC61_RS19065"
                     /old_locus_tag="HCC61_19060"
     CDS             2260..3792
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987789.1"
                     /locus_tag="HCC61_RS19065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19060"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_169136832.1"
                     /sec_met_domain="Aldedh (E-value: 1.8e-44, bitscore: 146.6,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAAPVWSVDPRTGNRREQVGTEATPGDVDAAVRAANAAREPLAD
                     RTVRAALLRAAAAQLDAASEDILAAADAETALGRPRLTGELARTQAQLRAFADEIEDGA
                     YLGVTINHKDADATPPCPDLRRYKIPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPV
                     VVKAHPDHPATSELTGAALRRAAAEVGLPEDVLSVVHGFEAGVELVKHPLVAAAGFTGS
                     VRGGRALFDAAASRPAPIPFHGELGSLNPVVVTSAAAAERAEQIGEGLAGSMTMGTGQF
                     CTKPGFVLVPEGESGDRLLEALREAVSKSAQGPLLDGGMRDAFLAGLGARAGLDGVEAP
                     VAGGPGDDEHSVSAGFLTVAAERLGQQQYELLLEECFGPVTVIARYQDDDEVTAALSRL
                     PGNLTATVHLGEAESAGEGGRGARLLAALTPLAGRVLVDGWPTGVAVAPAQHHGGPYPA
                     TTSTSTSVGATAIERWLRPVAYQDAPEALLPPELHDANPLGLPRRVDGRREN"
     gene            complement(3983..4882)
                     /gene="thpD"
                     /locus_tag="HCC61_RS19070"
                     /old_locus_tag="HCC61_19065"
     CDS             complement(3983..4882)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="HCC61_RS19070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19065"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_169136834.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-40, bitscore: 134.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVATPRMDPVVWSGAGAPGPIAAADLA
                     DYERDGFLAVDQLITPDEVETYRRELERLTTDPEIRADERSIVEPQSDEIRTVFEVHKI
                     SEVFAGLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLP
                     QMHTVSVSIALTRNYDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDES
                     LAKFADAHGIKLFTGDAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENEALEPFA
                     APVRRPDFIGAREFNPVR"
     gene            complement(5001..5405)
                     /locus_tag="HCC61_RS19075"
                     /old_locus_tag="HCC61_19070"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141824.1"
                     /locus_tag="HCC61_RS19075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19070"
                     /product="ectoine synthase"
                     /protein_id="WP_169136836.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.7e-53, bitscore:
                     173.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHIKSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTKTSMWYANHIEAVLCVEGESELTNDETGEKHLITPGTMYLLDGHEKHTMRPITDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPDDA"
     gene            complement(5485..6753)
                     /gene="ectB"
                     /locus_tag="HCC61_RS19080"
                     /old_locus_tag="HCC61_19075"
     CDS             complement(5485..6753)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 1.8e-75.
                     Score: 246.0. Matches aSDomain:
                     nrpspksdomains_HCC61_RS19080_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="HCC61_RS19080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19075"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_169136838.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-80, bitscore:
                     265.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIIQPDLSVFETVESEVRSYCRGWPTIFDRAQGSHMYDEDGHTY
                     LDFFAGAGTLNYGHNNPVLKRALLDYLERDGVVHGLDMSTTAKRAFLETFQEKILKPRG
                     MNHKVMFPGPTGTNAVEAALKLARKVKERESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLGGTTDDFVWFERLLEDQGSGLNKPAAVIVESVQGEGGINVARP
                     EWLRALSELCERHDMLLILDDIQMGCGRTGAFFSFEEAGIKPDIITLSKSIGGYGMPLA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATAAFETYWTDSQMEKQTLSRGEQVENVLR
                     AISEEHADDVADFRGRGLVWGLEFHDKSRANAVAKRAFELGLLIETSGPQSEVVKLLPA
                     LTITPEELDEGLRVLARAVRETA"
     aSDomain        complement(5659..6666)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HCC61_RS19080_Aminotran_3.1"
                     /evalue="1.80E-75"
                     /label="HCC61_RS19080_Aminotran_3.1"
                     /locus_tag="HCC61_RS19080"
                     /protein_end="365"
                     /protein_start="29"
                     /score="246.0"
                     /tool="antismash"
                     /translation="DRAQGSHMYDEDGHTYLDFFAGAGTLNYGHNNPVLKRALLDYLER
                     DGVVHGLDMSTTAKRAFLETFQEKILKPRGMNHKVMFPGPTGTNAVEAALKLARKVKER
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLGGTTDDFVWFERL
                     LEDQGSGLNKPAAVIVESVQGEGGINVARPEWLRALSELCERHDMLLILDDIQMGCGRT
                     GAFFSFEEAGIKPDIITLSKSIGGYGMPLALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATAAFETYWTDSQMEKQTLSRGEQVENVLRAISEEHADDVADFRGRGLVWGLEFH"
     gene            complement(6930..7457)
                     /gene="ectA"
                     /locus_tag="HCC61_RS19085"
                     /old_locus_tag="HCC61_19080"
     CDS             complement(6930..7457)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="HCC61_RS19085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19080"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_169136840.1"
                     /transl_table=11
                     /translation="MPEGLTLDAPRLEDGAALWRIARDSRTLDLNSSYSYLLWCRDFAA
                     TSVVARDAGGEPVGFITGYVRPARPRTLVVWQVAVDEAARGRGLAAAMLDGLAARTARE
                     LGTDRIETTVTPDNTPSNRLFTSFAERHGAAAEKEVLFDAGVFPESGHEPEVLYRIGPV
                     ASPDGLAPVPAR"
     misc_feature    complement(7455..7469)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.36"
                     /tool="antismash"
     misc_feature    complement(7509..7525)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(7665..7680)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7675..7690
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7675..7692)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    complement(7840..7856)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
     gene            7975..9087
                     /locus_tag="HCC61_RS19090"
                     /old_locus_tag="HCC61_19085"
     CDS             7975..9087
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468153.1"
                     /locus_tag="HCC61_RS19090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_19085"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_169136842.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.5e-18, bitscore:
                     61.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDRGDLQILHIKGHVLAGPEDIRDELWVVDGRITYERPTGTSSR
                     AARDIVTARGWVLPGMVDAHCHIGLDQQGPVDESTSEKQALTEREAGALLLRDAGSPSD
                     TRWIDDREDLPRIIRAGRHIARTKRYIRNYAHEIEPGDLAAYVRREARRGDGWVKLVGD
                     WIDRGEGDLASCWPRWALDEAIAAAHEEGARVTAHCFAEDSLRDLVEAGIDCIEHATGL
                     TEETVPLFAERGVAIVPTLVNTATFPQLAEGGAKYPRWSEHMLRLHERRYETVRAAYDA
                     GIPVYTGTDAGGSLAHGLVAAEVAELVTAGIPAAEAVSAATWGARRWLGRPGLEEGAPA
                     DLLVFDADPRGDVRVLGDPRHVVLRGRAVR"
     misc_feature    complement(9797..9806)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 gtcgtctccg gcatgggcgg ctcgccgcac accctcgccg ccgacgccgc acacgcccgc
       61 gacctgggcg ccgccgagct gcggctctac catgcgggcc tggcccccga cgccgacctc
      121 gcggccgtcg ggcaggccct gcgcgaactg gcctcgccgc acacggccgg gtgacccgtc
      181 ggcgcgtgtc acatccgcgc gccccgcccc gtcgtagacg tgtcagccgg ggcacacggt
      241 ccggcaggac gacgcgaacc agggacgaag gagcaccggt catggcacgc atctcgctcg
      301 acccgccgcg cagcgccatg ctgcgcatcg ccgcgtggta ctcccgccgc aagtacggca
      361 aggtcatgga cccgctgcgg gccgcggggc acaacccgaa ggtgctgggc gccgtgagcc
      421 tctacgagtc gcgcgtcgcg aagtggcggg ccctcgaccc cggtctgaag cacatcgccg
      481 agctggtcac cgcctcccgc atcggctgct cctggtgcgt ggacttcgga tactggctcg
      541 ccgacgacct gggcatgccg atggagaaga tccgttacgt gcctgcctgg cgggagcacc
      601 gcgagcgctt caccgaactg gagctgctcg tcatggagtt cgccgaggcg atgacggaga
      661 ccgagcccgc cgtgaccgac gggatgacgc gggagctgat cggcaggctg ggcgaaccgg
      721 ccttcgtcga gctgaccgcg atcgtggccg tggagaacca ccggtcccgc atcaacgccg
      781 cgctggggct ggtcggtcag ggcttctccg accagtgcgc ggtgcggccc gtggagcccg
      841 cggggaaggg cggcgcttcc ggaccggcgg actcctgacg gctggggctc gcgcgccgga
      901 acgctgcgcc gcggctcaac tgcccccgga caccggcgtg tcccagctcc acgcctcctc
      961 ggcgacgacc gccgagacgg gcaacggccc gtagatgtgc gggaattcct cgacggcgcc
     1021 gccgtccggc gtcccgctgc cgccgtcgtc ggcacaggcg gcggcaggct ccggcgcctc
     1081 gtaacggacg ggcacggaga gccgttcgct gtcgatgacg agcagcacga gctcggccgg
     1141 gtcccagccg ccgtacaaca tgcgtgccac tggcggcagt tggtggcgct gcgaacagtg
     1201 gatgaagccg acctcggcca gggtgtggcc gcgcgtggac atctcgtacg tgcccgactc
     1261 gcgggccgcc tcccagaggg accgctccgt caggtgcaac aactcagcca tgccgccaag
     1321 ttaccctccg gcgggcgccg ctcacccgtc gccggggtgc ctccgtctca cagccgccgc
     1381 gtcgccagcg tcagccggtc ccgtgcgtcg aagagcgcgt ccttgatcgt ctgctcgtgc
     1441 gccggggtga gtctggcgac cggcaccgag cagctgatgg cgtcccgcgc cggcgtccgg
     1501 taggggatgg ccacgccgaa gcagcgaagt ccgagcgtgt tctcctcgcg gtcgacggcg
     1561 tagccctgct cacgtacgag cgccagctcc tcgatcagcg cctcgcggtc ggtgagggtg
     1621 ttctcggtga gcgaggagag cgtctccggg agcatcttgc gcacctgctc gtcgtcgtac
     1681 gtggcgagca gcgccttgcc cagggaggtg gagtgggcgg gcagccgccg ccccacgcgc
     1741 gtgaagggac gcagatagtg ctgcgactgg cgggtggcga gatagacgac gttcgtgccg
     1801 tccagccgcg ccatgtgaat ggtctccgtg gtgtcgtcgg agagccggtc gagggtgggc
     1861 cgggccgcgg ccaccgcctc gtcgccgtcg atgtaggagg tcccgaccag cagggcgcgt
     1921 acgccgatcc cgtaccgtgt gcccgtggcg tcggtctcca cccagcccag atcgacaagc
     1981 gtgcgaagca gcatgtagag actggacttg ggatagccga cggcttcctg gacatcggcg
     2041 agggagtgca tccccggccg tcccgcgaag aactccagca gctccacagt gcgcacggcc
     2101 gacttgacct gcgatgcgct cgtctcgcca gctgacatcg tccttgaccc ctctgttgcg
     2161 gcgctcctag gctccccggc aggttcactg acagagatgt tgttcagcat atcgaacggc
     2221 ctctctgcgg agacaagcac gcggcaggag gaaatcacgg tggcagcagc accagtgtgg
     2281 agcgtcgacc cccggaccgg aaaccggcgt gaacaggtcg gcacggaggc cacacccggc
     2341 gacgtggacg cggccgtacg ggcggcgaac gccgccaggg agccgctcgc cgaccgcacc
     2401 gtgcgcgcgg ccctgctccg cgcggccgcc gcgcagctcg acgcggcgag cgaggacatc
     2461 ctcgccgcgg ccgacgcgga gaccgcgctc gggcggcccc ggctcaccgg tgaactggcc
     2521 cgtacgcagg cgcagttgcg tgccttcgcc gacgagatcg aggacggcgc gtacctgggc
     2581 gtcaccatca accacaagga cgcggacgcg acgccgccct gccccgacct gcgccgctac
     2641 aagatcccgc tcggcgtcgt cgccgtctac gcggcctcca acttcccgct cgccttctcc
     2701 gtgccgggcg gcgacaccgc cagcgccctc gccgcgggct gccccgtggt ggtcaaggcg
     2761 cacccggacc acccggcgac gtccgagctg acgggtgcgg cgctgcgccg tgccgcggcc
     2821 gaagtcgggc ttcccgagga cgtgttgagc gtcgtgcacg gcttcgaggc cggtgtcgag
     2881 ctcgtcaagc acccgctggt ggccgccgcg ggcttcaccg gttccgtacg cggcgggcgc
     2941 gccctcttcg acgccgccgc gtcacgtccc gcacccatcc ccttccacgg cgaactcggc
     3001 tcgctcaacc ccgtcgtggt cacgagcgcg gcggcggccg agcgcgccga gcagatcggc
     3061 gaggggctgg ccgggtcgat gaccatgggc accggccagt tctgcaccaa gccgggcttc
     3121 gtgctcgtgc cggagggcga gtcgggcgac cggctgctgg aggcgctgcg ggaggccgtg
     3181 agcaagtccg cgcaggggcc gctgctggac ggcggcatgc gggacgcctt cctcgccggt
     3241 ctcggcgccc gtgcgggact cgacggagtg gaggcgcccg tggccggcgg cccgggcgac
     3301 gacgagcact cggtgagcgc cggattcctc accgtcgccg cggagaggct cggacagcag
     3361 cagtacgagc tgctgctgga ggagtgcttc ggccccgtca cggtcatcgc gcgctaccag
     3421 gacgacgacg aggtcaccgc ggccctctcc cggctgcccg gcaacctcac cgcgaccgtg
     3481 cacctcggcg aggccgagtc cgcgggcgag ggcggccgtg gcgcaaggct gctggcggcg
     3541 ctgacgccgc tcgccggccg cgtactggtc gacggctggc ccacgggcgt cgccgtcgca
     3601 cccgcgcagc accacggcgg cccgtatccg gccacgacct ccacgtcgac atcggtgggg
     3661 gcgacggcga tcgagcgctg gctgcggccc gtcgcctacc aggacgcgcc cgaggcgctg
     3721 ctgccgccgg agctgcacga tgcgaacccg ctggggctgc cgcggcgtgt ggacggacgc
     3781 cgggagaact gaaccacgcg cgtgtaagtc gcctcgttcg ggccgctgcc ggggccgttc
     3841 cgggacctcg ctccgggacg ccgttccggg acccgaagaa gcccggaacg ggcccgaaga
     3901 gtgcggagaa aagccgaaaa ggggggagac cgcagcgcgc ggcctccccc tcttcagcgt
     3961 gtgcggagcg tgacgccccg catcacctca cggggttgaa ttcgcgcgcc ccgatgaagt
     4021 cgggacggcg gaccggcgcg gcgaagggct ccagggcctc gttctccacg ctgttgaaga
     4081 cgatgaaaac attgctccgc ggatacggag tgatgttgtc accgctcccg tgcatgcagt
     4141 tgcagtcgaa ccaggtcgcg gagccggcat cgcccgtgaa gagcttgatg ccgtgtgcgt
     4201 cggcgaactt ggccagggac tcgtcggagg gagtccccgc gtcctgcatc tgcaaggact
     4261 tcttgtagtt gtccttcggc gtctcgcccg cgcagccgag gaaggtcttg tgtgaccccg
     4321 gcatgatcat caggccgccg ttggtgtcgt agttccgtgt cagtgcgatc gagaccgaga
     4381 cggtatgcat ctgcggaaga ccgtcttccg cgtgccaggt ctcgaagtcg gagtgccagt
     4441 agaagccagt ggcaccgaag ccgggcttga cgttgatcct ggactggtgg acgtagacgt
     4501 ccgagcccag gatctgtcga gcccggccca caacacgcgg gtcccgcacc aggccggcga
     4561 agacctcgct gatcttgtgc acctcgaaca cggtccggat ctcgtccgac tggggctcga
     4621 cgatcgagcg ctcgtcggca cggatctccg gatccgtggt gagccgttcc agttccctgc
     4681 ggtacgtctc gacctcatcg ggggtgatga gctgatcgac cgcgaggaac ccgtcccgct
     4741 cgtagtccgc cagatccgcg gccgcgatgg gccccggagc acccgcgccg gaccacacga
     4801 cggggtccat gcgtggggtg gccacctcgg tggctccccg agtcgggtac aggtcggcgg
     4861 tgcgctcggg tgcggtggtc atggtgttgc cttcctctcc tctcgtacgg ccctgttcgg
     4921 tgggcgagcg gccgaagccg cgggcggcgc cggagccgcc ggacgagcct gagcaggccg
     4981 agccgtgacg ggctcagtcg tcaggcatcg tcaggctctg tgagaagcgg atagacgccg
     5041 ttctcgtcgt gatcttcccg gcccgttacg ggcggattga agacacagat gcaccggaag
     5101 tcggtaatcg ggcgcattgt gtgcttctcg tgcccgtcga gcagatacat ggtgccgggg
     5161 gtgatcagat gcttctcacc ggtctcatcg ttggtgagtt cggactcgcc ctctacgcag
     5221 agtacggctt cgatgtggtt tgcgtaccac atggaagtct tcgtcccggc gtacagcacg
     5281 gtctcgtgca gggagaagcc cacacgctcc ttcgccagca cgatccgctt gctttcccag
     5341 gtgccggatg cggatttgat gtgacggtcg gtgttctcga tgtccttgaa agaacgaaca
     5401 atcacgttct gactgacctt tctctattca ttacggggtc ggcccgcccc ggtcgcaacc
     5461 ggagcgggcc gtgagcggag tcggctaggc ggtttcgcgc accgccctcg ccagcacacg
     5521 cagaccctcg tccagttctt ccggagtgat cgtaagcgcc ggaaggagct tcacgacctc
     5581 actctgggga ccggaggtct cgatgagcag accgagttcg aaggcgcgct tggcgacggc
     5641 gttcgcacgg gacttgtcgt ggaactccag gccccacacg agcccgcggc cgcggaagtc
     5701 ggcgacatcg tccgcgtgtt cctcggagat ggcgcgcaac acgttctcga cctgctcgcc
     5761 gcgggagagc gtctgcttct ccatctggct gtcggtccag tacgtctcga acgcggcggt
     5821 ggcggtgacg aaggccggat tgttgccgcg gaacgtgccg ttgtgctcgc ccggctccca
     5881 cacgtccagc tcgggcttga acagcgtgag cgcgagcggc atcccgtaac cgccgatgga
     5941 cttggacagc gtgatgatgt ccggcttgat gcccgcctct tcgaaggaga agaaggcgcc
     6001 ggtgcggccg cagcccatct ggatgtcgtc gaggatgagc agcatgtcgt gccgctcgca
     6061 cagttcggac agggcgcgca gccactccgg gcgcgcgacg ttgatgccgc cctcgccctg
     6121 cacgctctcg acgatcacgg cggcgggctt gttcaggccg gagccctggt cctccagcag
     6181 ccgctcgaac cagacgaagt cgtcggtggt gccgcccaga tagtcgtcga agggcatcgg
     6241 cgtgccgtgc accagcggga tgccggcgcc ggcgcgcttg aaggcgttgc cggtgacggc
     6301 gagcgagccg agcgacatgc cgtggaaggc gttggtgaag gagacgatgg actcgcgctc
     6361 cttgaccttg cgggccagct tcagcgcggc ctcaacggcg ttggtgcccg tcgggcccgg
     6421 gaacatcacc ttgtggttca tgccgcgggg cttgaggatc ttctcctgga aggtctccag
     6481 gaaggcgcgc ttggcggtgg tgctcatgtc gaggccgtgc acgaccccgt cgcgctccag
     6541 gtagtccagc aacgcccgtt tcagcacggg gttgttgtgc ccgtagttga gggtcccggc
     6601 tccggcgaag aagtcgaggt acgtatggcc gtcctcgtcg tacatgtggg agccctgggc
     6661 ccggtcgaag atcgtgggcc agccgcggca gtagctgcgg acttcggact cgacggtctc
     6721 gaagacactg aggtcgggct ggatgatggt cacggcatgc tcctgggagt gagtcgcgtg
     6781 cgaagttcgt gggggcttcg gggagtcggg gcggtctctg gaagtcggtc actggtcggg
     6841 gtctggattc tgcggatcgg ccgcggggcc acggcgtcgc gcacggtccg tgcctccggg
     6901 gcgccgaacg gcgccgagag cacgcgggtt cagcgcgcgg gcaccggggc gaggccgtcc
     6961 ggggacgcca ccgggccgat ccggtacagc acttcgggct cgtgaccgct ctcggggaac
     7021 accccggcat cgaagagcac ctccttctcc gccgccgccc cgtgacgctc cgcgaaggag
     7081 gtgaagaggc ggttggaggg ggtgttgtcc ggggtgacgg tggtctcgat cctgtcggtc
     7141 cccagctccc gtgccgtacg ggccgcgagc ccgtcgagca tcgccgccgc cagtccgcgg
     7201 ccgcgtgccg cctcgtcgac tgcgacctgc cagacgacca gggtccgcgg gcgtgcgggg
     7261 cgtacgtagc cggttatgaa accgaccggc tcaccgcccg cgtcacgcgc caccacggag
     7321 gtggcggcga agtcgcggca ccacaggagg tagctgtagg aggagttgag atcgagcgtc
     7381 cgggagtcgc gggcgatgcg ccagagcgcc gctccgtcct ccagccgtgg agcgtcgagt
     7441 gtcagccctt ccggcatttc ctggaaatct ctgtgggcac gtgcatggtc tgcttgtgcg
     7501 gcggtcatgg gtattaaatt tacccagcag aatctgaaaa cgcacggcgg gacggggtta
     7561 cgcaagaggg gggtcctgtg ttatcgcgcg caggcgcgca cgtgagctca ggggcaccgt
     7621 ttcgccctgg catatcagca ctttccgggg cacaacgggc gtcctgtgga gtccgtcaca
     7681 ttgccgtaac gaccttgacc cggaggcgaa ttacgccacg ataaccggtg aaaaactaag
     7741 cgtttaacga agcggaaagc gggcagaaga atacgggcag ctacgctctt catagccacg
     7801 ggaaatgcat tctcccgtat cggctccgat gtcgcatctt tccgccgggt atgcaatgcg
     7861 gtcacctttt ggatcaccac cgtactggat caccactgtg cgtatgtgcc cgaagcgcgg
     7921 tgtccttgtc cggcgggtgc agcccgaggc ggcagggccc atagggtgcg gagcatgaac
     7981 gaccgcggcg atctccagat cctccacatc aaggggcacg tgcttgccgg gcccgaggac
     8041 atacgggacg aactgtgggt ggtggacggg cgcatcacct atgaaaggcc cacgggcact
     8101 tcctcacggg ccgcccgcga catcgtcacc gcacggggct gggtgcttcc gggcatggtc
     8161 gacgcgcact gccacatcgg cctcgaccag cagggccccg tggacgagtc gaccagcgag
     8221 aagcaggcac tgaccgagcg ggaggccggt gcgctgctgc tccgcgacgc gggctccccg
     8281 tccgacaccc ggtggatcga cgaccgcgag gacctgccgc gcatcatccg cgccggccgg
     8341 cacatcgccc gtacgaagcg ctacatccgc aactacgcgc acgagatcga gcccggcgac
     8401 ctcgccgcgt acgtgcggcg cgaggcgcgg cgcggcgacg gctgggtgaa gctcgtcggc
     8461 gactggatcg accgcggtga gggcgacctc gcctcatgct ggccccggtg ggcgctggac
     8521 gaggccatcg ccgctgcaca cgaggaaggc gcccgtgtca ccgcgcactg cttcgccgag
     8581 gactccctgc gcgacctggt cgaggccggc atcgactgca tcgagcacgc cacgggtctc
     8641 accgaggaga ccgtcccgct cttcgctgag cgcggcgtcg ccatcgtccc gaccctcgtg
     8701 aacacggcca cgttcccgca gctcgcggag ggcggcgcca agtatccgcg ctggtcggag
     8761 cacatgctcc ggctccacga gcggcgctac gagaccgtgc gcgccgccta cgacgcgggc
     8821 atccccgtct acaccggcac cgacgccggg gggtcgctgg cgcacggact ggtcgccgcc
     8881 gaggtcgccg aactggtcac ggccgggatc ccggcggccg aggcggtctc cgcggcgacg
     8941 tggggcgcgc gccgctggct cgggcggccg gggctggagg agggcgcccc ggcggacctg
     9001 ctggtcttcg acgccgaccc gcgcggggac gtacgggtgc tgggggaccc gcggcatgtg
     9061 gtgctgcggg ggcgggcggt gcgctaggcc gtgtccggcc gctcggtccg atgtggcggg
     9121 cggcgatcgg cgccgggcag aggcggggcg gcgtccggcg gtcggcgtcc ggctggggca
     9181 gggcggtgcg ctgtgtaccg ggcgccatcg gcccggaggc cgggggcgga ggaggaagga
     9241 gcgtcgtgca ggagatcgtc tcgtgggacg cccgcgaact gtggcgtgcc gtcgcggacc
     9301 gccgtgtctc gtgcgtcgag gtcatggagg cgtacctgga gcacatcggc aggatcggcc
     9361 cgcacgtcaa cgcagtcgtc gggctccagg caccggagac gtcgctgcgg ctggcccgtg
     9421 agcgggacga gcagctgcgg cgcggcgaac cgccggggcc gctgcacggt ttcccgcacg
     9481 ccgtgaagga cctgaccgag gccgaggggc tgccctggac ggagggctcg ccgctgtacc
     9541 gggaccgcgt cgggaagcag gacgcgctgc tcgtgaagcg cgtcaaggcc gcgggcgccg
     9601 tggtgatcgg caagacgaac acccctgaat tcgggctggg ttcgcagacg tacaacgagg
     9661 tgtggggcgc ggcggggaac gcctacgatc cctcccgcac ggcgggcggc agcagcggcg
     9721 gcgcggcggc ggcgctcgcc ctgcggatgc tgccggtcgc ggacggcagc gactacatgg
     9781 ggtcgctgcg caacccggcc gccttcaaca acgtcttcgg catgcgtccg agctggggtc
     9841 tcgtgccctc gcccgggttc acggcgcagc tggggactgc ggggccgatg gcccgtacga
     9901 cggccgacct ggcccggctg ctgtcggtca tggccggacc ggacgggagc gcgccgctgg
     9961 cggcggggca ggcacagggc gagcgcttcg cggagagcct cgaacgcccg gagggcgcct
    10021 tccgcggcac ccgcatcgcg tggacggggg actgggacgg gtatctggcc acggagccgg
    10081 gcgtgctgga ggtgtgccgc tcgtcgttcg ccgcgttcga ggccctgggg tgcgaagtca
    10141 ccgacgcacg gccggacttc gcgccggagg agatctggcg gacgtttctg acctggcgct
    10201 ggtgggcgaa cctggagcgg cacgagctgt acgcggatcc cgcgacgcgg gcgcggatga
    10261 agcccgaggc cgtgtgggag atcgagtgcg gactgccgct cggcgcgctc gacatcgcgg
    10321 aggcggcggt cgcacgtgac cgctggtacg aggcgctgcg gggcttcctc gacgactacg
    10381 actacgtgct cgccccggcc gcgca
//
