LOCUS       NZ_JAASAG010000002     11008 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. HNM0575 scfid2, whole genome shotgun sequence.
ACCESSION   NZ_JAASAG010000002
VERSION     NZ_JAASAG010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. HNM0575
  ORGANISM  Streptomyces sp. HNM0575
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 934398)
  AUTHORS   Xiao,K.
  TITLE     New actinomycetes from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 934398)
  AUTHORS   Xiao,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) College of Tropical Crops Hainan University,
            Hainan University, Renmin Avenue, HaiKou, HaiNan 570100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2018
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012726265.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 05:29:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,992
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,797
            CDSs (with protein)               :: 5,797
            Genes (RNA)                       :: 69
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 15 of 126
            Pseudo Genes (incomplete)         :: 115 of 126
            Pseudo Genes (internal stop)      :: 5 of 126
            Pseudo Genes (multiple problems)  :: 8 of 126
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 180775
            Orig. end                         :: 191783
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASAG010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11008
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11008
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11008
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6008](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6008
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    840..853
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.96"
                     /tool="antismash"
     gene            complement(1175..1960)
                     /locus_tag="HCC61_RS04685"
                     /old_locus_tag="HCC61_04685"
     CDS             complement(1175..1960)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842350.1"
                     /locus_tag="HCC61_RS04685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04685"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_169133997.1"
                     /transl_table=11
                     /translation="MTAYTDSTSEANWQAWQDSWDRQQEFYLPDREERFRVMLDMTEAL
                     VGPEPRVLDLACGTGSISARVLRRFPAARCTGVDLDPALLTIARGSFEGDERVEFVSAD
                     LTGPEWTRALPHRSYDAVLTATALHWLRLPQLSALYGQLAGVLREGGVFMNADHMPDGS
                     TPRISEAAHAFARIRWERSGAGGSKPWIDWWQRIAGEPELADAVAERYRIFGNPVNGDH
                     ADGEVQSAGWHAETLRASGFAEARQVWCSPEDGMVLGLR"
     gene            2063..2701
                     /locus_tag="HCC61_RS04690"
                     /old_locus_tag="HCC61_04690"
     CDS             2063..2701
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326935.1"
                     /locus_tag="HCC61_RS04690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04690"
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="WP_169133998.1"
                     /transl_table=11
                     /translation="MELSYYSDLAVRLVNTEQPERDEDTLTSVEAVRELCGGNRQTARR
                     ATEADVARLRAVRTRLRAVFTAAGEGDETRAVDLLNALLMEFPTSPRISGHGDRAADGR
                     PRWHMHLAEHPVTAAAGYAATACIGLAFHLTDLGVDRLGICQAAACSNAYLDTSTNRSR
                     RYCSDRCATRANVAAYRARKRATDRAADGGATSGRTADSAHSSTGRSER"
     gene            complement(2596..3054)
                     /gene="sodX"
                     /locus_tag="HCC61_RS04695"
                     /old_locus_tag="HCC61_04695"
     CDS             complement(2596..3054)
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016485 - protein processing [Evidence
                     IEA]"
                     /GO_process="GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sodX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207366.1"
                     /locus_tag="HCC61_RS04695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04695"
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="WP_346777026.1"
                     /transl_table=11
                     /translation="MRGYGERRNGAVSGSGGLLKRIGLARVYNPSMLPTLRPGDRLVLK
                     YGSRVRPGAVVVVRHPLQHDLLIVKRAVERRAGGWWVIGDNPQVRNDSREFGPVPDDLM
                     VARAWVRLRPPRGPQRSLRPVLLWALSAVRPLVAPPSAARSVARLRAR"
     gene            3241..3636
                     /gene="sodN"
                     /locus_tag="HCC61_RS04700"
                     /old_locus_tag="HCC61_04700"
     CDS             3241..3636
                     /EC_number="1.15.1.1"
                     /GO_function="GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sodN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054839.1"
                     /locus_tag="HCC61_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04700"
                     /product="superoxide dismutase, Ni"
                     /protein_id="WP_028434210.1"
                     /transl_table=11
                     /translation="MLSRLFAPTVKVSAHCDLPCGVYDPAQARIEAESVKAIQEKYQAN
                     DDPHYRARATTIKEERAELAKHHVSVLWSDYFKPPHFEKYPDLHQLVNETLKALSTAKG
                     STDPATGQKALDYIAQIDKIFWETKKG"
     gene            complement(3812..4585)
                     /locus_tag="HCC61_RS04705"
                     /old_locus_tag="HCC61_04705"
     CDS             complement(3812..4585)
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005870.1"
                     /locus_tag="HCC61_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04705"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_169134504.1"
                     /transl_table=11
                     /translation="MVVSNVPHSARMWDYWLGGKDHYEVDRLAGEKAREAYPGIVDIAR
                     TSRALLSRVVRYLIAEAGVRQFLDIGTGLPTADNTHEIAQRAAPESRIVYVDNDPLVLT
                     HARALLTSAPEGATDYLHADVNEPGPILEEAARTLDLSRPVGLIMFGIIGHVPEHSDAV
                     SIARGLMDGLAPGSHLALYTGTDADPDLVEAERQVGEHGGVPYRLSNLEQVEEFFAGME
                     LVEPGVVPGTRWRPEGEVVESAADVASLGGLARKP"
     misc_feature    4592..4606
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(4626..4641)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.05"
                     /tool="antismash"
     misc_feature    complement(4913..4928)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.67"
                     /tool="antismash"
     gene            5001..6008
                     /locus_tag="HCC61_RS04710"
                     /old_locus_tag="HCC61_04710"
     CDS             5001..6008
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068156.1"
                     /locus_tag="HCC61_RS04710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04710"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_169134000.1"
                     /sec_met_domain="AfsA (E-value: 3.9e-20, bitscore: 67.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAGDPQGLSYATTVSRQLVHRAAVCEVFLTDSVRLSDTEFHVAA
                     QLPRVHSYYSDHAASPASYDPLLLLEVFRQTSILLTHKHLSIPQDSRFVFNSGDLRVID
                     TDALRVGPLPGHAVVDAAVVAEKRRDGQLVGVTLEMRLAIDERRAASMTMSIQWMPPGV
                     WNGLRERGRAGLDLSSPRPPSVGSRLTPESVGRTSPRNVVLREAVVLDTEVVAQVEVDQ
                     SHPALFDHPLDHIPGALLFEACRQTAVHAAHELLGLSPRLLTLHRCAAEFTRFGEFELA
                     TTCRAELVEDPERSALVFELQMLQDEAVISTAQIALKCTSPAGQRVVHAAAAAP"
     misc_feature    5499..5501
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6127..6783
                     /locus_tag="HCC61_RS04715"
                     /old_locus_tag="HCC61_04715"
     CDS             6127..6783
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068155.1"
                     /locus_tag="HCC61_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04715"
                     /product="HAD family phosphatase"
                     /protein_id="WP_169134001.1"
                     /sec_met_domain="HAD_2 (E-value: 0.00013, bitscore: 17.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPPMLWFDFGGVLSPPLDDLYDAYQAKTGIAPEQLKGAIKEVAA
                     AMDLPPLAPVELALLSEAEWGGRLRRVLSDRHPGLDLSRARLESFGEQWFDGVEANPVM
                     VCALRYARDNGFRVGVLSNNVVEWEEHWRRIIAPAGDLDCLVDSCKAGHRKPEAAVFDL
                     AARTAGADPGDCVLIDDLAENCDAARARGWRAVHFRQNRQALADLQQLTGLPSLL"
     misc_feature    6271..6273
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6835..8034
                     /locus_tag="HCC61_RS04720"
                     /old_locus_tag="HCC61_04720"
     CDS             6835..8034
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068154.1"
                     /locus_tag="HCC61_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04720"
                     /product="cytochrome P450"
                     /protein_id="WP_169134002.1"
                     /sec_met_domain="p450 (E-value: 6e-15, bitscore: 50.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATPAVTTTDIPHLDPPPAYRAHSTRSPGGLPQVVLPSGHTAV
                     HLTRYADVHKVLTDATFARAETNVETGPSFLPTVMPPELLLNLDVPDHARMRGFVAADY
                     SAAGVESLRPTVEAVIAERFTELRAAAAPDLFRTVLDPLPVTVNCRLLGIPASDIASFR
                     PYARTVQMASAEDISHLLDHFWKVYGYVTDLVTGARPVQPGGLVDRFVTGRDSAEPPLS
                     DEELTGLLLGSLLGADQNILSVLTKAVYALLCAPPLWRRLVDDPDLASRMTEELIRLMP
                     LGTISAFPRVATRDVAVGDGVIGTGEVVYADAFAANRDPRAFPDPLTIDPDRAGKRHLQ
                     FGYGMHHCMGAALARMQIPLVLARLAQEFPGLALDAEPRSLPWDHGVLLRRPTALPVRW
                     "
     misc_feature    6871..6885
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     misc_feature    7285..7287
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8039..9832)
                     /locus_tag="HCC61_RS04725"
                     /old_locus_tag="HCC61_04725"
     CDS             complement(8039..9832)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106290.1"
                     /locus_tag="HCC61_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCC61_04725"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_169134003.1"
                     /transl_table=11
                     /translation="MSASGAGSSGFGGPGSGAAGSDAPGASDAPGTPRTTVPGTTAPGE
                     PRHFEVAIVGGGPVGMLLAAELGLHGVRTVVLERNPATVDQPKAGTLHARTVQSLLRRG
                     YLPAPRDGRPYARATAPFHFAGLPGLVISAPASEGPPVVGRSQADLERAFERRARELGA
                     EVLRGHEVTALEQDDRRVLLTARDLHGQPVRVSALHAVGADGARGVVRTLTGIPSDETP
                     PTLAALLGSVRLGDPGGTPPGWRLTDRGWTVINVDPYGHSRVITFDFTGPHEDRGAPLG
                     LEELQATAGRIAGRAIPMTDLVFASRFSDYSRLVRTYRSGRVFLAGDAAHVHFPVGGQG
                     LNLGLQDALNLGWKLALTLRGFAGDRLLDTYHEERHPAGARVVENTRAQLALMRPGAET
                     APLRALFSELLGLEEVRSHLGDMISAQETSYGMPGEPSGQGRFLPNVPLTVGGRATSVA
                     EELHAARPVLLIFGANGSGTGGLAGEAAPWAAAVDVVHATSSVRMPWDAVLLRPDGYLA
                     WTPTGPVPEPGGLRAALTHLFGEPPAARAAAGGRSERDVQGVHGTQGGRAVQAVRSAGA
                     QGAQSPTQSPTQRPTQNPQRP"
     misc_feature    complement(9872..9888)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.19"
                     /tool="antismash"
ORIGIN
        1 cggaggaact gtccctcgcg tacacgcccg gcgtcgccaa gatttgcagc gccatcgccg
       61 aacagccgga gctggtcgcg gactacacct ggaagtcgca ggtggtcgcc gtggtcacgg
      121 acggcagcgc ggtgctcggt ctgggcgaca tcggagcgga ggcgtccctc ccggtgatgg
      181 agggcaagtc cattctcttc aagcagttcg gcggcgtgga cgcgttcccg gtcgcgctga
      241 actgccgcga gaccgacgag atcgtggaga ccgtcgcacg tctcgcaccg tccttcggcg
      301 gcgtcaacct ggaggacatc gcggcgccgc ggtgcttcga gatcgaggcc aggctcaagg
      361 agcggctgga cgtccccgtc ttccacgacg accagcacgg cacggccatc gtcacgctcg
      421 cggcacttcg caacgccgcg ctgctgacga agcggacgct gggcgacctg cgtgcggtca
      481 tctccggcgc gggcgcggcg ggattcgcga tcgccaagat cctcgtggag gcgggcatcg
      541 gcgacgtcgt cgtctgcgac cgcaagggcg tcgtctccga ggaccgcggc gatctcacgg
      601 acgtcaagcg ggagttggcc cggttcacca acaaggaggg acgcaccggc tcgctggagt
      661 cggtgatggc cggcgccgac gtcttcatcg gcgtcagcgg cggtacggtc ccggaggagg
      721 cggtggcgac gctggccgag gactccttcg tcttcgccat ggccaacccc aacccggaga
      781 tccacccgga cgtcgcctcc aagtacgccg cggtcgtcgc gacgggccgc agcgactacc
      841 cgaaccagat caacaacgtg ctcgccttcc ccggtgtctt cgcgggcgcc ctccaggtgc
      901 gggcgacgga catcaccgag gggatgaagc tcgcggccgc cgaggccctg gcggccgtgg
      961 tctccgacga gttgagcccc gagtgcgtga tcccctcgcc gttcgacgag cgcgtcgccc
     1021 ctgccgtgac ctcggcggtc gcggcggccg cgcgcgcgga aggcgtcgcg cggcgctgac
     1081 cggtcgccgg ccggcgccaa ggagcaagta cgcgaatcag gaagggcggt gggggcggaa
     1141 acgcccccac cgcccttccg cgttccggac gccgtcagcg cagcccgagc accatcccgt
     1201 cctccggcga gcaccacacc tgccgcgcct cggcgaagcc cgacgcccgc agcgtctcgg
     1261 cgtgccagcc ggcggactgg acctctccgt ccgcgtgatc gccgttcacc gggttcccga
     1321 agatgcggta gcgctcggcg accgcgtccg ccagctccgg ctcaccggcg atccgctgcc
     1381 accagtcgat ccagggcttc gagccccctg cgccgctccg ctcccagcgg atccgggcga
     1441 aggcgtgagc ggcctcgctg atgcgcggcg tggagccgtc cggcatgtgg tcggcgttca
     1501 tgaagacgcc gccctcgcgc agcacgcccg ccaactgccc gtacagcgcg ctcagttgag
     1561 gcaggcgcag ccagtgcagc gccgtggccg tgagcacggc gtcgtacgag cggtgcggca
     1621 gcgcgcgcgt ccactcgggg ccggtcaggt ccgcgctcac gaactccacg cgctcgtcgc
     1681 cctcgaagga cccccgcgcg atcgtgagca gcgccgggtc gaggtcgact cccgtgcagc
     1741 gtgccgcggg gaagcgccgc agcacccgcg cggagatgct gcccgtgccg caggccaggt
     1801 ccagcacccg cggttcgggt cccaccagcg cctcggtcat gtcgagcatg acccggaagc
     1861 gctcctcgcg gtcgggaagg tagaactcct gctgccggtc ccaactgtcc tgccacgcct
     1921 gccagttcgc ctcgcttgtg ctgtccgtgt acgcagtcac gccgccgtcc tcctgccggg
     1981 tggaaacgga tgtaataccc tcgcaccgat cgcactcatt accgacacgg tcgacgatag
     2041 accggtaccg taaggaccac aagtggaact cagctattac tcggacctgg ccgtgcgcct
     2101 ggtcaacacc gagcagcctg agcgcgacga ggacaccctc acgtcggtcg aggccgtacg
     2161 ggagctgtgc ggcggcaacc gccagacggc ccgccgcgca acggaggccg acgtggcgcg
     2221 tctgcgcgcc gtacggaccc gcctccgggc cgttttcacc gctgccggcg agggcgacga
     2281 gacccgcgcc gtggacctgc tgaacgccct gctgatggag ttccccacga gcccccggat
     2341 cagcggccac ggcgaccgcg ccgccgacgg gcgcccccgc tggcacatgc atctcgccga
     2401 gcacccggtc acagccgcgg ccggctacgc cgccacggcc tgcatcggcc tcgccttcca
     2461 cctcaccgac ctcggcgtgg accggctggg catctgccag gccgcggcgt gcagcaacgc
     2521 gtacctggac acctcgacga accgctcccg gcgctactgc tcggaccgct gcgcgacccg
     2581 cgccaacgtg gccgcctacc gcgcccgcaa gcgcgccacg gaccgtgccg ccgacggcgg
     2641 cgccaccagc ggccgcaccg ccgacagcgc ccacagcagc acgggccgca gcgagcgctg
     2701 aggcccccgc ggcggacgca gccgcaccca cgcgcgcgcc accatcagat cgtccggcac
     2761 cggcccgaac tccctgctgt cattgcgcac ctgcggattg tcaccgatca cccaccagcc
     2821 ccccgcacgg cgctccacgg cccgcttgac gatgaggaga tcgtgctgga ggggatggcg
     2881 caccacgacc acggccccgg ggcgcacgcg cgagccgtac ttcagcacca gccggtcgcc
     2941 gggacgcagc gtgggcagca tcgacgggtt gtagacccgg gcgagcccga tccgcttcag
     3001 caaaccgccc gaaccgctca cggccccgtt cctccgctct ccgtagcctc gcaccgcccg
     3061 ctcccgatcc gcgtccggca cttcctgcgc cggccgcatc cgtgccgctc acctcgtccc
     3121 cgtcctcacg ttcacccaac accggacttt tgtcctaagc ccccgggggc acacgcgaaa
     3181 gggcctcccc tacggagtaa tctcacaggt gagaagacga tcacgaggaa ggacggctct
     3241 atgctctccc gtctgttcgc gcccacggtg aaggtcagcg cccactgcga cctgccctgc
     3301 ggtgtctacg acccggcgca ggcccgcatc gaggcggagt cggtcaaggc catccaggag
     3361 aagtaccagg ccaacgacga cccgcactac cgcgctcgtg ccaccacgat caaggaggag
     3421 cgggccgagc tggccaagca ccacgtctcc gtgctgtgga gcgactactt caagcctccg
     3481 cacttcgaga agtacccgga cctgcaccag ctggtcaacg agacgctcaa ggcgctctcc
     3541 accgcgaagg gctcgaccga cccggccacc gggcagaagg ccctcgacta catcgcccag
     3601 atcgacaaga tcttctggga gacgaagaag ggctgaaacc gcaacggacg tccggcactt
     3661 cgggccggac gagcccgatc cgcccctgta cgacgggact cgaccgtcgt acaggggcgg
     3721 atcgctgagc ggcgccgggg caatcgcagg ggcccgccgg gcagttcggg gcccggtcgg
     3781 tcagggcccg ggaacgccgg gccccggagc gtcagggctt gcgtgcgagg ccgccgagcg
     3841 aggcgacgtc cgccgccgac tcgacgacct ctccctcggg acgccagcgg gtcccgggga
     3901 ccacgcccgg ctcgaccagc tccatgcccg cgaagaactc ctcgacctgc tccaggttcg
     3961 agagccggta ggggactccg ccgtgctccc ccacctgccg ctccgcctcg accaggtcgg
     4021 ggtcggcgtc ggtgcccgtg tagagcgcga gatgactgcc gggggcgaga ccgtccatga
     4081 ggcctcgtgc gatggagacg gcgtcggagt gctcgggcac gtgcccgatg atgccgaaca
     4141 tgatcaggcc caccgggcgg gacaagtcga gtgtccgcgc ggcctcttcg aggatcgggc
     4201 ccggttcgtt gacgtcggcg tgcagatagt cggtggcgcc ctcgggcgcg ctggtcagca
     4261 gcgcgcgggc gtgtgtgagc accagcgggt cgttgtccac gtagacgatg cggctctcgg
     4321 gcgcggcccg ctgcgcgatc tcgtgggtgt tgtcggcggt gggcagcccc gtgccgatgt
     4381 cgaggaactg ccggactccc gcctcggcta tgaggtaccg caccacccgg ctgagcagcg
     4441 cccggctcgt acgcgcgatg tcgacgatgc cgggatacgc ctcgcgtgcc ttctcaccgg
     4501 ccagccggtc gacctcatag tggtccttgc cgccgagcca gtagtcccac atgcgtgccg
     4561 agtgaggcac attgctgaca accatcggga catcacgact gacgatcagc gggacgtcaa
     4621 cccctggttc gccgccggct tccgcgtcgt ccgccatctc cgcacgctcc tcgctgtgtc
     4681 gacggtcacg tgatgctagc gcacggcgcg cagtgcggta ccggcccacc tcggagcggc
     4741 aactcggccc ggcgggaggg gagttgtaca tgcccgcacg tgccgcaccc ccgcggccgc
     4801 cacaacaagg cggctcctgc ggcgagttgg gcggtggccg gcttcggagc gtgccggcga
     4861 aacgcccgca aaccgcccgg caatgaactc ttaactcaca ggacgcaact cgtgtgactc
     4921 ccggcacagc ctaattccat cgaaccttgg tacgttcagt ttcgaaccta tcgccatgcc
     4981 gaacgaaaac ggggatcgaa atgtccgcag gtgacccaca aggtctgtca tacgccacga
     5041 ccgtctcccg ccagctggtt caccgggccg cagtctgcga agtcttcctg acggactcgg
     5101 tgcggctctc cgacacggag ttccatgtcg cggcccaact gccgcgagtg cacagctact
     5161 acagcgatca cgccgcgagc cccgcgtcct acgatccgct gcttctcctg gaggtcttcc
     5221 gccagacctc gatcctgctc acgcacaagc acctgtccat accccaggac agcaggttcg
     5281 tcttcaactc cggcgatctg cgggtgatcg acacggacgc gctgcgcgtc ggcccgctgc
     5341 ccgggcacgc cgtggtcgac gccgccgtcg tcgccgagaa gcgccgcgac ggccaactcg
     5401 tcggcgtgac cttggagatg agactggcca tcgacgagcg gcgcgcggcg tcgatgacga
     5461 tgtccatcca gtggatgccg cccggagtgt ggaacgggtt acgggagcgg ggccgtgcgg
     5521 ggctcgatct gagcagcccg agaccgccct ccgtcgggag caggctcact cccgagtcgg
     5581 tcggccgcac ctcgccgcgc aacgtggtgc tgcgcgaggc cgtggtcctg gacaccgagg
     5641 tcgtggccca ggtcgaggtc gaccagtcgc accccgcgct cttcgaccac ccgctggacc
     5701 acatcccggg ggcgctgctc ttcgaggcct gccggcagac cgccgtacac gccgcgcacg
     5761 aactgctcgg tctctccccg cgcttgctga cgctccacag gtgcgccgcc gagttcaccc
     5821 gcttcgggga gttcgaactg gccaccacct gccgggccga gctggtggag gaccccgaac
     5881 gctccgcgct cgtcttcgag ttgcagatgc tccaggacga agccgtcatc tcgaccgcgc
     5941 agatcgccct gaagtgcacc agccccgccg ggcagcgcgt cgtccacgca gccgccgcgg
     6001 cgccgtagga cgacggcaga gcgctgagaa ggcctgagcg cccgccgcat ctctctcccg
     6061 gtgccacatc tctcctggtg ccgcctcgct ttcccggtgc cgccctcctc agagaaggga
     6121 acccccatgt ccccacccat gctctggttc gacttcggcg gggtcctgtc cccaccgctg
     6181 gacgacctct acgacgcgta ccaggccaag accggcatcg cccccgagca actcaagggg
     6241 gcgatcaagg aggtcgccgc ggcgatggac ttaccgccgc tcgcgcccgt cgaactcgcc
     6301 ctgctgagcg aggccgagtg gggaggccgg ctcagacgcg tcctgtccga ccgccacccc
     6361 ggcctcgatc tctcccgtgc acggctggag agcttcgggg agcagtggtt cgacggtgtc
     6421 gaggccaacc ccgtcatggt gtgcgcactg cggtacgcgc gtgacaacgg attccgcgtg
     6481 ggcgtcctgt cgaacaacgt cgtggagtgg gaggagcact ggcgccggat catcgctccc
     6541 gccggtgacc tcgactgcct cgtcgactcc tgcaaggccg gccaccgcaa gccggaggcg
     6601 gccgtcttcg acctggcggc acggacggcc ggcgcggacc ccggggactg cgtgctcatc
     6661 gacgatctcg ccgagaactg cgacgcggca cgtgcccgcg gctggcgcgc ggtgcacttc
     6721 cgccagaacc ggcaggcgct ggcagacctc cagcaactca ccggtctccc gagcctgttg
     6781 tgaccgtccc tcgcccgttg tcaggaagcc cgcccgttgt caggaaggca gcccatgacc
     6841 accgccaccc ccgccgtgac gaccacggac atcccgcacc tcgatccccc gcccgcctac
     6901 cgcgcccaca gcacccgttc tcccggcggc ctgccgcagg tcgtcctgcc cagcgggcac
     6961 accgccgtgc acctgacccg ctacgccgac gtccacaagg tgctcaccga cgcgacgttc
     7021 gcccgcgccg agaccaatgt cgagaccggc ccgagcttcc ttccgacggt catgccgccg
     7081 gagctgctgc tcaacctcga cgtccccgac cacgcgagga tgcggggctt cgtcgccgcc
     7141 gactacagcg cggcgggagt ggagtcgctc cgccccaccg tggaggcggt gatcgccgaa
     7201 cgcttcacgg agctgcgcgc cgccgcggcc cccgacctct tccgtacggt gctcgatccg
     7261 ctgcccgtca ccgtcaactg ccgcttactg ggcatcccgg cctcggacat cgcctccttc
     7321 cgcccgtacg cccgcacggt gcagatggcc tccgccgagg acatatccca cctcctcgac
     7381 cacttctgga aggtctacgg ctatgtgacg gacctggtga cgggagcccg gcccgtgcag
     7441 cccggcggac tcgtcgaccg cttcgtcacc ggacgggaca gtgcggaacc accgctcagc
     7501 gacgaggagt tgaccggtct cctgctcggc tcgctgctcg gcgccgacca gaacatcctg
     7561 tccgtgctga ccaaggcggt gtacgcgctg ctgtgcgcgc cgccgctgtg gcggcgcctc
     7621 gtcgacgatc cggacctcgc gtcgcgcatg acggaggaac tgatccgtct gatgccgctg
     7681 ggcaccatct ccgccttccc ccgtgtcgct acccgcgatg tcgcggtggg cgacggcgtg
     7741 atcggcaccg gagaggtcgt ctacgccgac gcgttcgccg ccaaccggga cccgcgggcg
     7801 ttccccgacc ccctgaccat cgatccggac cgggccggca agcgccacct ccagttcgga
     7861 tacggcatgc accactgcat gggggccgcg ctggcccgga tgcagatccc gctcgtactc
     7921 gcccggctcg cccaggagtt ccccggcctg gccctggacg cggaaccccg ctccctgccg
     7981 tgggaccacg gcgtactgct gcgccgcccc acggcgctgc ccgtccgctg gtgacggcct
     8041 acgggcgctg cgggttctgc gtggggcgct gcgtggggct ctgcgtgggg ctctgcgcgc
     8101 cctgagcacc agcactccgc acggcctgga cggcccggcc gccctgtgtg ccgtggacgc
     8161 cctggacgtc ccgctcgctc cgtcccccgg cggccgcccg tgccgccggg ggctcgccga
     8221 acagatgcgt aagcgccgca cgcagacccc ccggctcggg caccggcccc gtcggcgtcc
     8281 acgccagata gccgtccggg cgcagcagga cggcgtccca gggcatcctc accgacgagg
     8341 tcgcatggac gacgtcgacg gccgcggccc acggcgccgc ctcgccggcc aggccgccgg
     8401 tgccggagcc gttcgccccg aagatcagca ggaccggccg ggccgcgtgc agctcctcgg
     8461 ccacggacgt ggcccgaccg cccacggtca gcggcacgtt gggcaggaag cggccctgcc
     8521 cggaaggctc gccgggcatg ccgtacgacg tctcctgcgc gctgatcatg tcgccgaggt
     8581 gggagcggac ctcttcgagt ccgaggagct cggagaagag cgcgcgcagc ggtgcggtct
     8641 ccgcgccggg cctcatcagg gcgagctggg cacgggtgtt ctcgacgacg cgggccccgg
     8701 ccgggtgccg ttcctcgtgg tacgtgtcga gcaggcggtc cccggcaaag ccgcgcaggg
     8761 tcagcgcgag cttccagccc aggttcagcg cgtcctgaag ccccaggttg agcccctggc
     8821 ctcccaccgg gaagtggacg tgcgccgcgt cgccggccag gaagacgcgc ccgctgcggt
     8881 aagtgcgcac gaggcgcgag tagtcgctga agcggctcgc gaagaccagg tcggtcatgg
     8941 gaatggcccg gcccgcgatg cggccggcgg tcgcctgcaa ctcctccagg ccgagcggag
     9001 cgccgcggtc ctcgtgcggc ccggtgaaat cgaaggtgat cacgcggctg tgcccgtacg
     9061 gatcgacgtt gatcaccgtc cagccccggt cggtgagccg ccacccggga ggcgtgccgc
     9121 cggggtcgcc gaggcgcaca ctgcccagca gggcggccag cgtcggcgga gtctcgtcgc
     9181 tggggatgcc ggtgagcgtc cgtacgacgc cgcgtgcgcc gtcggcaccg acggcatgga
     9241 gggcgctcac gcgcaccggc tgcccgtgca ggtcccgggc cgtcaagagc acccgccggt
     9301 cgtcctgttc gagcgcggtc acctcgtggc cgcggaggac ctccgccccg agttcgcgtg
     9361 cccgccgctc gaaggcgcgc tccagatcgg cctgggaccg tccgacgacg ggcgggccct
     9421 cgctcgccgg tgcgctgatg accaggcccg gcagcccggc gaagtggaag ggcgcggtgg
     9481 cccgtgcgta cgggcggccg tcgcgcggag ccggcagata accgcgccgg aggagggact
     9541 gcaccgtacg cgcgtgcagc gtcccggcct tgggctgatc gacggtggcg gggttgcgct
     9601 ccagcacgac ggtgcggaca ccgtgcagcc ccaactccgc ggcgagcagc atcccgacgg
     9661 gaccgccgcc gacgatggcg acctcgaagt gccgcggctc cccgggtgcc gtggtacccg
     9721 ggaccgttgt gcgcggagtg cccggtgcgt ctgaggcgcc cggggcgtcc gatccggcag
     9781 ccccggatcc gggaccgccg aatccggaac tgcccgcccc cgacgcggac atggcgcgcc
     9841 ccgcgctcaa cgggcgccgt gcggccggtc gttgcggccc gtgcgcagca gcaccgtgat
     9901 gacggcgagg aggaccaggc agacgacggc caggatccac agcacggtgt ggaacgcgga
     9961 gtcgtaggca ccggcgagga agtcggtgaa ccggccgcgc tcgcctgccg cgacggcctg
    10021 cgccggtccc gacacatcgc ctccggcgac ctggttggcc acgcgcgacg ggtcgccggc
    10081 gtccgcgtag gcgccgatgc cctcccgtac gcgggcggac agcacggtgg ccaggaggga
    10141 cccgtagacg gccacggcga ccgcttcgct gcccagccgc agcgtgttga ggaagcccgc
    10201 cgccatgccg gccttcccgg gctccacgat ctccagcgcc tgtccgtcga ccagcccggc
    10261 ggacagcccc atccccgcgc ccgtcacgag catcggcacg gccacgagga cgacggccac
    10321 gtccgcgctg aacaggttca gcgcgatgtc gccgacgacc aggcacacca ggctgagccg
    10381 gatcaccgtc aacgcccgta cgccgcggga gaccagcctg gccgcgagca tcgggcagag
    10441 cagaaccggg agggtgagca gcaccatcac cagtccggcc ttgccgctgc tgtaaccggt
    10501 gaccgtcgtc agatagctcg gaaggtacgt gagcatcgtg acgaatccga aggacgcggc
    10561 gaccggcacg aggcacagcg cgagaaagcg gcggtcggcc atgaggccca ggtcgagcat
    10621 cggggacgag tggcgcttct ccaccacggc gaaggccgcg aggaccaccg ccgcgcccgc
    10681 gaagaggccc agcaccccgg gaccggccca gccccactgc gagccctgga cgattccggt
    10741 ggtcagcaag gccatggccg ccacgaacag ggcgctgccg gcgacgtcga tccgggcgcc
    10801 ctcacggcgc gcggcggggg tgttgcgtgc catggcgggc acggcgagga gcacgacggc
    10861 cagggcggcg gcgtgcaggc cgaagaccga gcgccagccc aggccttcca cgagcaggcc
    10921 ggagagcgac ggcccgacgg cgaccccggc accggcgacg gtgccgaaaa gggcgaacgc
    10981 cctggcacgt gccgccccgt cgaacacg
//
