LOCUS       NZ_CP051206            20831 bp    DNA     circular CON 09-DEC-2024
DEFINITION  Dolichospermum flos-aquae CCAP 1403/13F chromosome, complete genome.
ACCESSION   NZ_CP051206
VERSION     NZ_CP051206.1
KEYWORDS    RefSeq.
SOURCE      Dolichospermum flos-aquae CCAP 1403/13F
  ORGANISM  Dolichospermum flos-aquae CCAP 1403/13F
            Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Dolichospermum.
REFERENCE   1  (bases 1 to 5151752)
  AUTHORS   Wu,V.
  TITLE     Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial
            Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5151752)
  AUTHORS   Fomenkov,A., Anton,B.P. and Roberts,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2020) Genome Biology, NEB, 240 County Rd, Ipswich,
            MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-DEC-2016
            Assembly Method                   :: HGAP v. 2.3.0
            Assembly Name                     :: Afl_HGAP3_4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114.08x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012516395.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:24:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,921
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 66
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 35 of 187
            Pseudo Genes (incomplete)         :: 162 of 187
            Pseudo Genes (internal stop)      :: 14 of 187
            Pseudo Genes (multiple problems)  :: 21 of 187
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:01:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4861068
            Orig. end                         :: 4881899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051206.1.
            Bacteria and source DNA available from CCAP 1403/13F.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20831
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="15"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20831
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20831
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10831](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10831)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    643..659
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 23.0"
                     /tool="antismash"
     gene            complement(650..1060)
                     /locus_tag="HGD76_RS23245"
                     /old_locus_tag="HGD76_23250"
     CDS             complement(650..1060)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861913.1"
                     /locus_tag="HGD76_RS23245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23250"
                     /product="hypothetical protein"
                     /protein_id="WP_015079964.1"
                     /transl_table=11
                     /translation="MNAFQPSKPPLQPVEQRRTVPRPKRHLRQRAYQIMALETTAKVAV
                     NIVISAAAVSALVQLLPYHWSQQDKLRTVRTEVKVMEGRVQNLQTEFSRNFDPGEAKSI
                     MQQKSFRFDPNQRPVILMNQEQTGTEQSNSAP"
     misc_feature    complement(1151..1165)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.63"
                     /tool="antismash"
     gene            complement(1304..1399)
                     /gene="psaM"
                     /locus_tag="HGD76_RS23250"
                     /old_locus_tag="HGD76_23255"
     CDS             complement(1304..1399)
                     /GO_component="GO:0009538 - photosystem I reaction center
                     [Evidence IEA]"
                     /GO_process="GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /gene="psaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141228.1"
                     /locus_tag="HGD76_RS23250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23255"
                     /product="photosystem I reaction center subunit XII"
                     /protein_id="WP_006196319.1"
                     /transl_table=11
                     /translation="MPISDTQVYIALAVALIPGILAWRLATELYK"
     misc_feature    1419..1433
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.81"
                     /tool="antismash"
     gene            1482..1694
                     /locus_tag="HGD76_RS23255"
                     /old_locus_tag="HGD76_23260"
     CDS             1482..1694
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS23255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_23260"
                     /product="hypothetical protein"
                     /protein_id="WP_168697167.1"
                     /transl_table=11
                     /translation="MLQDATKTGDLSVVHCPLSLGRNIPLLPLLPLPPLPPLLPLPPLL
                     PLPPLLPLLPLPPLLPLIYDFIAWY"
     gene            1672..2964
                     /locus_tag="HGD76_RS23260"
                     /old_locus_tag="HGD76_23265"
     CDS             1672..2964
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1210: glycerol kinase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079965.1"
                     /locus_tag="HGD76_RS23260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23265"
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="WP_168697168.1"
                     /transl_table=11
                     /translation="MTLSLGIDFGTSGARGVVIDEEGRILSEMRHCWQVGTVDWLKCWQ
                     QALWSLLGQIPGVLRGEIGAIAINGTSSTILLTDGAGKPVDAPLLYNDARGAVVLERLS
                     RIAPANHTVLSATSSLAKLLWMQELPDFAKARYLLHQADWLGFLLHGKLGISDYHNALK
                     LGYDVEKLQYPAWLEQLEIPLQLPQVLAPGTAIGELLPQIAADFGFKGDCLVVAGTTDS
                     IAAFLASGAKFPGEAVTSLGSTLVLKLLSRHRIEDARYGIYSHRLGDLWLTGGASNTGG
                     AILKHFFSDVELVRLSAEIDPTQASVLDYHPLLKPGDRFPINDPQLLPRLEPRPDQPQE
                     FLHGLLESMARIETRGYELLQNLGADPLTHIYTAGGGAANSTWTAIRQRYLKVPIVSSK
                     NTEAAYGTALLAMGNKLIEKRTTEDTEDAEE"
     gene            3030..3734
                     /locus_tag="HGD76_RS23265"
                     /old_locus_tag="HGD76_23270"
     CDS             3030..3734
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079966.1"
                     /locus_tag="HGD76_RS23265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23270"
                     /product="hypothetical protein"
                     /protein_id="WP_168697169.1"
                     /transl_table=11
                     /translation="MVMNLITKLVSQMYNNFKFPQILVFGLIITSLFACGLDVTENSTS
                     ANAKNPEQSQLNQNTLPKSISQAVLRDASRVSGIKSSDLQITQVTSTNFSNPCAFKFGE
                     VCTREYNPIPGWIVIVQVQKQSWTYHVNKSGSQILLDPKISSNQLPKKIANAVLSNASK
                     RSGLAVNSLKITQSIQKTFSNSCVFNFGEVCTQNFAPIKGWEVIVKVKSQFWTYHVDIT
                     GTRLVLDPQGMD"
     misc_feature    complement(3768..3785)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            3849..4268
                     /locus_tag="HGD76_RS23270"
                     /old_locus_tag="HGD76_23275"
     CDS             3849..4268
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS23270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_23275"
                     /product="hypothetical protein"
                     /protein_id="WP_168697170.1"
                     /transl_table=11
                     /translation="MLEAIANGFYQDEKLEQLPAGLQGYYESHWRIMGMTNKPLPKDKI
                     KIIYAICFAKTAISDDLIAKHSKQSNLKVQEVIRDWTQFLQIQKQENNQPYLYRFYHES
                     FRDFLQKQDIVKAAGVTLSEVKKEISDNISEGLYD"
     gene            4261..5517
                     /locus_tag="HGD76_RS23275"
                     /old_locus_tag="HGD76_23280"
     CDS             4261..5517
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1173: WD-40
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012618.5"
                     /locus_tag="HGD76_RS23275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23280"
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="WP_168697171.1"
                     /transl_table=11
                     /translation="MTKFIDWFNQLSEEGKKYYLGCLPETLLDGKDYTKLYKLLSDYEF
                     ITTKINHPLFGIQALIEDYDLLDNSKVRYNSEYAETVKALKLIQGTLSRSAHIIFQDSK
                     QLKGQLSARLTHFDLPEIKNLLAQIATDKNIGLYSLMGSLIPPGGGGLIRTLEGHSYSV
                     EAIAVTPDGKTVISGSDDKTIKIWDLRTGTEKFTLTGHSDWVNAIAVTPDGKTVISGSV
                     DKTIKIWDLVTGTEKFTVKGHSDFVNAIAVTPDGKTVISGSVDKTIKIWDLVTGTEKFT
                     LKGHSYSVKVIVFTPDGKTVISASTDNTIKIWDVETGTEKFTLKGHSSWVSAIALAPDG
                     KTLISASRDNTIEIWDLATRKEIATSTGESPINCCTVAPDGVTIVAGEESGRLNLLLEN
                     LSSTPPQPSPQLRWIYCGF"
     gene            5496..5717
                     /locus_tag="HGD76_RS23280"
                     /old_locus_tag="HGD76_23285"
     CDS             5496..5717
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS23280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_23285"
                     /product="hypothetical protein"
                     /protein_id="WP_210967678.1"
                     /transl_table=11
                     /translation="MDILRVLTAEETPAMSTVAISKIIKADVFDVAEIMETWLEFLQEI
                     QTNKGTKYQLYHHSFRLYLHDYSTLRNI"
     gene            complement(5824..6678)
                     /locus_tag="HGD76_RS23285"
                     /old_locus_tag="HGD76_23290"
     CDS             complement(5824..6678)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876604.1"
                     /locus_tag="HGD76_RS23285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23290"
                     /product="EndoU domain-containing protein"
                     /protein_id="WP_233466969.1"
                     /transl_table=11
                     /translation="MMLNLLSKTINVQSCKDLHEFRYLILAAELFFITTLFSPSLVSAE
                     EFLPFFDNTKNPVKVKFGGVQDLTPPPPVLNKFDKAVNQLCGEVGTVVKPASFKTMFNS
                     FPDVFSNIKNNLGGYIFPKRTSNQEFLEDLTNLWFNAKGFDHVFCGEPEKNKIGGLHFA
                     GRYLDLQNKRIAGILPKSVSTSEVKPGAVYSFGVRMKVGGSTVNDPLKGYGYTLNAEEI
                     LTIAAKAYKNNPNSETTSKACLLTVTDDNKTFVNVFVAKKGGVRTFYPDATPDYQKNPE
                     CQV"
     gene            complement(6783..7841)
                     /locus_tag="HGD76_RS23290"
                     /old_locus_tag="HGD76_23295"
     CDS             complement(6783..7841)
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080968.1"
                     /locus_tag="HGD76_RS23290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23295"
                     /product="J domain-containing protein"
                     /protein_id="WP_168697172.1"
                     /transl_table=11
                     /translation="MARKTKTASSTTVTPLALSDLHLQLAGLEKEHQSLLKQIKKKRTE
                     LNNFVEKMRSLATEVFHRVSPNMKTMAELDAEIHALFAEILNTRKMGKQTQKNIQSLYR
                     SLQMGGIISYKPIEEEEDDDDDEELDELFEDNDAQENHQRRRQFWEAEQDSESPTVART
                     DESRKIRQTFLRLAEIFHPDKVKDNETQMTHTEIMKEINKAYQDGDLARLLEIERKYEV
                     GETIDNNSEDDLSRRCKNIEQHNQILKNQYEKLKQELRLAKNTPEGSMVADYKKAAKQG
                     IDCIELMLETIQSQTKIVAEIRDFVQDFKDKKITIKEFFAGPESLRSVQEDMMEELLEK
                     MMEDFGGMMDFN"
     gene            complement(7926..8657)
                     /locus_tag="HGD76_RS23295"
                     /old_locus_tag="HGD76_23300"
     CDS             complement(7926..8657)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080965.1"
                     /locus_tag="HGD76_RS23295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23300"
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="WP_325064819.1"
                     /transl_table=11
                     /translation="MVSGDAAQEAIALAKNHPHLAVGLHLVLVCGKSVLPPAQIPNLVD
                     SQGNFSNNPTQAGLSYQFNQATRAELRLEISAQLEKFRDSGLNLAHVDGHLHLHVHPVI
                     LNILTEFAAEFQIKFIRLPSEELSKNLKIDNRNLFTKIIWSIVFGQLRRYGEVLLKANQ
                     IKFADRVYGLLQTGDMSEEYLLGLIPQIEAELVEIYSHPDLVNTEINNGGEVELAALLS
                     QQVREMLTVKGFKLSNYNQLI"
     gene            complement(<8701..9777)
                     /gene="hpnJ"
                     /locus_tag="HGD76_RS23300"
                     /old_locus_tag="HGD76_23305"
                     /pseudo=""
     CDS             complement(<8701..9777)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860052.1"
                     /locus_tag="HGD76_RS23300"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23305"
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /pseudo=""
                     /sec_met_domain="PF04055 (E-value: 6.9e-16, bitscore: 57.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTTLFLSPPSFDGFDGGAGARYQAKREITSFWYPTWLAQPAALV
                     PGSRLVDAPPHGQTVDDVLKIAKDYELVIMHTSTPSLANDVKCAETIKAQNPNVQIGFV
                     GAHVAVLPEATLRENPVIDFVCRNEFDYTCKELAEGKPWDEIKGLSYRDKLGNIHQTEE
                     RPLIHDWDAMPSVLPVYARDLDISKYFIGYLQHPYISFYTGRGCPAKCTFCLWPQTIGG
                     HLYRHKSPDAVGREMEEAKVLFGDKVREYMFDDDTFTIDKHRAIAISKHMKRLNLTWSC
                     NARANLDYDTLKTLRDNGLRLLLVGFESGNQEVLNNIKKGIKLEVAREFMKNCHKLGIT
                     VHGTFIIGLPIETKETVSD"
     gene            complement(10001..10831)
                     /locus_tag="HGD76_RS23305"
                     /old_locus_tag="HGD76_23310"
     CDS             complement(10001..10831)
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130356.1"
                     /locus_tag="HGD76_RS23305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23310"
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="WP_168697173.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.1e-25,
                     bitscore: 88.7, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 1.6e-08, bitscore:
                     32.3, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLRGDALQILKETSRTFYIPISILPSGLQEAVASAYLCMRAIDQ
                     IEDDPNLDNETKKRLLQNISLTLQAGVDGFPLDAFSVGFKGYENSLQEVSLRIREWSIL
                     APESIAPRIWDATAAMADRMAYWSDRNWQIETEADLDRYTFGVAGAVGLLLSDLWNWYD
                     GTQSNRTQAIGFGRGLQAVNILRNNGEDLTRGVNFYPAGWDNDHLQKYARRNLILADAY
                     TNSLPNGPALQFCQIPLALAHGTLDALASGKEKLSRSDVVSLIENLMGVNAKAS"
     gene            complement(11476..12378)
                     /locus_tag="HGD76_RS23310"
                     /old_locus_tag="HGD76_23315"
     CDS             complement(11476..12378)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF017256.5"
                     /locus_tag="HGD76_RS23310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23315"
                     /product="GUN4 domain-containing protein"
                     /protein_id="WP_325064846.1"
                     /sec_met_domain="Pkinase (E-value: 1.2e-10, bitscore: 39.3,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPQNILLRQNKQEAVLIDFGLTRSIATKSMTNALTEGYAPIEQY
                     RRKGSFGSHTDVYALAATLYYLLTADGLNKEDEVSPVPAQNRKYDDEPLPAPKHYNSRI
                     SQRVNDAILAGMEIEPENRTPTVLKFRENLGLVVQQPINFDIQQPINVETFHGTSLQTS
                     NMDYRKLRDYLAQGKWKEADGETKRVTLAVTKREKEGWLDVESINNFPCEDLRTIDHLW
                     VKYSDGKFGFSVQKRIYQSLKTREDSDIWEKFGDKVGWRKGGRWLYYTDITFDKKAPEG
                     HLPVFEWRRGGVLLSRRDL"
     gene            complement(12374..12529)
                     /locus_tag="HGD76_RS25665"
     CDS             complement(12374..12529)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS25665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_233466970.1"
                     /transl_table=11
                     /translation="MIIWQPGQEINNGRFIIQGKPLGSGGFGTTYKALEPSTGKLYRQR
                     QFTPRH"
     misc_feature    12763..12777
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 25.64"
                     /tool="antismash"
     gene            complement(12921..14297)
                     /locus_tag="HGD76_RS23315"
                     /old_locus_tag="HGD76_23320"
     CDS             complement(12921..14297)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080962.1"
                     /locus_tag="HGD76_RS23315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23320"
                     /product="DUF697 domain-containing protein"
                     /protein_id="WP_233467279.1"
                     /transl_table=11
                     /translation="MLDSWHDSIVQVGEFGLLSALAVGGGLWLFNKNQPQLGEQLNDIV
                     VNRADVDKAIAKTQVIINQLAQEAANHPHLAILRETLAKLPLELDRKEITLAVTGGKSV
                     GKSTVIEVLKTAPTIPGISLHFAETAPLFSVAGENSDVVTLSEMQKSDFVLFLTNGDLT
                     DSEFQVLQQLKATKQPSLLVFNKQDQYQPDERATVFQSLKQRIGANVVATAASPVPVKV
                     RKHEEDGSFQEWMEQPTPDIQQLTQQLAAVVGQQGEQLVCNTTNRKVLLLKAEAKNCLN
                     GVRRDQATPFIEQYQWIAAAAAFANPVPALDILATAAITAQMVIDLGNIYQQKISLEQA
                     QQVAGTMGSLMLKLGLVELSTRAVTGILKTNVATFVAGGMVEGVSAAYLTRVAGLSLVA
                     YFEQQEIALESGSALNLDKLRQVLQTVFQQNQKMAVLEAFVKQGVKRLLPEAKPVEVVA
                     "
     gene            14625..15683
                     /locus_tag="HGD76_RS23320"
                     /old_locus_tag="HGD76_23325"
     CDS             14625..15683
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320416.1"
                     /locus_tag="HGD76_RS23320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23325"
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_015080960.1"
                     /transl_table=11
                     /translation="MNKTASQLVLTFGILALTSSFTNTLGLAQNIQKPNEATKVTNSAT
                     DALIKVDGSSTVYPITQSIAKAYQANLKNNSQIQVNISGTSGGFEKFCTRKIDISNASR
                     PINQSEMAACKKNGVSYMELPIAFDAVTIAINPQNTWAKDITVAELKKIWEPAAEGKIT
                     KWNQVRDSWPDRPIKLYGPGGKSGTYDYFTEAIVGKAKASRKDYTASENDEVLVTGVNK
                     DPNALGYFGYAYYQENKDKLKVLAVNSGKGAVLPSPETVEKAKYQPLSRPLFIYVNLRS
                     SKNKTLVYNLADFYIKNAAKIVSSVGYVPLSEEAYKLNSIHLYKGKAGTVFGGKSEFNL
                     SLGQLLRKQKQF"
     gene            15699..16715
                     /locus_tag="HGD76_RS23325"
                     /old_locus_tag="HGD76_23330"
     CDS             15699..16715
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080959.1"
                     /locus_tag="HGD76_RS23325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23330"
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="WP_168697174.1"
                     /transl_table=11
                     /translation="MTIRVGINGFGRMGRLSVRAAWDYPEIEFVHINETKGGTEAAAHL
                     LKFDSVHGRWTPEVEAVKNQVVIDGKSLSFTEYSQPGEVPWEDFGVDIVLECSGKFRTP
                     ATLDPYFKRGVQKVIVAAPVKQEALNIVMGVNDHLYQADKHHLLTAASCTTNCLAPVVK
                     VIHEGLGIKHGVITTIHDNTNTQTIVDAPHKDLRRARATSMSLIPTSTGSATAIGLIYP
                     ELNGKLNGLAVRVPLLNASLTDCVFEVERSTTIAEVNDLLKTASAQKPLIGILGYEERP
                     LVSIDYKDDPRSAIIDALSTMVINETQVKILAWYDNEWGYVNRMTELARKVALSLQK"
     gene            16818..18080
                     /gene="arsJ"
                     /locus_tag="HGD76_RS23330"
                     /old_locus_tag="HGD76_23335"
     CDS             16818..18080
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="arsJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080958.1"
                     /locus_tag="HGD76_RS23330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23335"
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="WP_168697175.1"
                     /transl_table=11
                     /translation="MASTTASNSNFKNYILVTLAYWGFTLTDGALRMLVLLYFNKIGYT
                     PIQIASLFLFYEVFGIVTNFLGGWIGSQFGLKVTLYTGIGLQVFSLIMLSFLNPGWAQW
                     LSVLYVMVAQAFSGIAKDLTKMSSKSAIRLVVPQDAQSSLFKWVAVLTGSKNALKGVGF
                     FLGSALLSSVGFINSLWIMAGGLALIMFSGLMLPKAMGKIKKKVKFSQLFSKSAEINIL
                     SAARFFLFGSRDVWFVVALPVFLRGTLGWSFYQVGGFLACWVIGYGIIQFLAPTLMQRF
                     GSGRPPQSQTIRFWTFTLTAVPGAIALALQLGLPANIVIIAGLLVFGVVFAFNSAVHSY
                     LVLAFTDDDKVALNVGFYYMANSGGRLVGTVLSGLVYQIFGLVGCLWTSMFFVFAAGLV
                     SLKLPNPQPSKAIAWKTGDGE"
     gene            18307..19383
                     /gene="arsB"
                     /locus_tag="HGD76_RS23335"
                     /old_locus_tag="HGD76_23340"
     CDS             18307..19383
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0015700 - arsenite transport [Evidence
                     IEA]"
                     /GO_process="GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /gene="arsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080957.1"
                     /locus_tag="HGD76_RS23335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23340"
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="WP_325064820.1"
                     /transl_table=11
                     /translation="MEYVTKQPTIKRLSFLDRFLTLWIFLAMATGVGLGYFFPTIEVFI
                     NHFQIGTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNKKILGLSLSLTWIIAPIVMFT
                     LAILFLRDKPEYMTGLIMVGIAPCIAMVIVWSELAKGDTEYTAGLVAFNSIFQILFYSV
                     YAWFFLAILPPLFGLQGTIVNISIGEIANSVFIYLGIPFLAGFMTRFILVKAKGKQWYH
                     QQFIPKISPITLIALLFTIMVMFSLKGNLIVQIPLDVVRIAIPLIIYFLFMFLSSFYLA
                     WKIKADYAKAASVAFTAAGNNFELAIAVAISVFGIKSGVAFAAVIGPLVEVPVLISLVS
                     VSFWIKKHFFRASPPIGL"
     gene            19423..20211
                     /locus_tag="HGD76_RS23340"
                     /old_locus_tag="HGD76_23345"
     CDS             19423..20211
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080956.1"
                     /locus_tag="HGD76_RS23340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_23345"
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="WP_168697176.1"
                     /transl_table=11
                     /translation="MKVADLITWFEEWANPAWCESWDNCGWQVEPGVLSTEARVLVCLT
                     PTLAVMQEAIALHANLIFAHHPLIFHPPKSFRTGEALTEMVRLAFTHNIGIYTAHTNFD
                     QVADGTADVLSQLLELQQVSPIVPTQAGLGYGRVGMLKPTLTLQEVINNIQTQLNPPHL
                     IFSPMADLQQQISRVAVLGGSGAGYISAVVKTNAQVYLTSDCKFHQFQESRDRGIILID
                     AGHYATERPACDRLVQKFQALNLDWVKLSQQDEDFRQFLP"
     misc_feature    complement(20277..20290)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.95"
                     /tool="antismash"
ORIGIN
        1 tgaccatcat cccgaaaatg cggtcgatta caataaccga gggctgattt attttcaaag
       61 tggtgatagc caaaaagcta tctctgatta taacaaagcc ttagatttaa attctaaatt
      121 agctagtgcc tacaataatc gggctaatta ttacgctgct tgtggtgaat taacagccgc
      181 attggctgat tatgaacaag cattagatct aaatccccat catgttcggg cttggattaa
      241 tcgcagtatt actttacgcg acttgggaga ctatgcagaa gctattgaaa acttagaaat
      301 tgccatgctg tttggtcaac tcaaaggcaa catttgggcg gaacagggac ggacttatca
      361 tctctggggt gattggaatt gtgcaatcgc gaattatcgt cgcgcattgg cagaactacc
      421 atctttaaat catcaagatg tttccggtta tcgcttacgg ttacaaatcg aaaattggtt
      481 aaacgagttg ctcttttttg aagactaatt tagtagatgg cttaattcag atttttagca
      541 ttcaattggt aaaactgact caaaaatttg agtttcatga ctaaatataa agttgtatca
      601 tttggtagtc accctaataa ccgtgatgat taatatattt tctgcacttt tatggtgctg
      661 agttagattg ttccgttcca gtctgctctt gattcatcaa tataactgga cgctgattag
      721 ggtcaaagcg aaatgatttt tgttgcataa tacttttagc ttccccagga tcaaagttgc
      781 ggctaaattc tgtctgtaag ttttgcacac gcccttccat aacttttact tcggtacgaa
      841 ctgttcgcaa cttatcctgt tgtgaccaat gataaggtaa taattgtact aaagcagata
      901 ctgctgctgc actaataact atattaactg ctactttggc tgtagtttcc agtgccatta
      961 tttgataagc acgttggcgt aaatgtcgtt taggtctggg tacagttcgg cgttgttcta
     1021 cgggctgtag aggaggttta gatggttgaa acgcattcat ggtgctaaca aaaaactcgc
     1081 tgatttacgg taattaccgt caaaagcaaa caggtgctaa ttgcggctta atttagctgc
     1141 catattactc cattttctgg aaatttatac aagtatgaag atgaataaaa tattacctag
     1201 taaagtaggg gcgattccta aaattcccat agtttagaac tacaagtatt tgaaatctac
     1261 tcctacttat ggattaagca cctgtaacag cgttacactg gtgttatttg tatagttctg
     1321 tagctaaacg ccaagctaga attcctggaa ttagtgctac agctaacgca atgtaaactt
     1381 gggtatctga gataggcatg atagaaatcc tcctaagttg aacttgattt tcaagactcc
     1441 gtttactact tttccctgtt ttgccaaaat aaggaaacat cttgttacaa gatgcaacaa
     1501 aaactgggga tttgtcagtt gttcattgtc cgttgtcatt gggaagaaat attcccctac
     1561 ttcccctact tcccctacct cccctacctc ccctacttcc cctacctccc ctacttcccc
     1621 tacctcccct acttccccta cttcccctac ctcccctact tcccttaatt tatgacttta
     1681 tcgcttggta ttgactttgg tacttctggc gcacggggtg tggtaattga tgaagaaggt
     1741 cggattctgt ccgaaatgcg ccattgttgg caggttggga ctgttgattg gctgaaatgt
     1801 tggcaacagg ctttatggag tctattaggg caaattccgg gggtgttaag gggggaaatc
     1861 ggagcgatcg ctattaatgg gacttcttcc actattttac tgactgatgg ggctggaaag
     1921 cctgtggacg cgccactatt gtacaatgat gctcggggtg cagtggtttt agaacgattg
     1981 agcaggattg cgccagcaaa tcacactgta ttaagtgcta catctagtct agcgaaactc
     2041 ctctggatgc aagaattacc tgattttgca aaagccagat atttattgca tcaagccgat
     2101 tggctgggat ttctcttgca tggaaagtta ggtattagcg attaccacaa cgccttaaaa
     2161 ttgggttatg acgtggaaaa attgcaatat ccagcatggt tggaacagct agaaattccc
     2221 ctccagttac cgcaagtctt agcgccgggt acagccattg gtgaattact tccccagatt
     2281 gcggccgatt ttggctttaa aggcgattgt ttagtggttg ctggcacaac agatagtatt
     2341 gcggcttttc tagccagtgg agctaaattt ccgggggagg cggtaacttc tctcggttca
     2401 actttggtac taaaactttt aagtcggcat agaattgaag atgcgcgata tggaatttat
     2461 agccatcgtt taggagattt gtggttgact ggaggtgcat ccaatacggg tggtgctatt
     2521 ttgaagcatt ttttcagcga tgttgagttg gtgaggttaa gtgcggaaat tgatccaact
     2581 caagccagtg tgttagatta tcatccatta ttgaaaccag gcgatcgctt tcccattaat
     2641 gatccccaat tattacccag actagaacca cgtccagatc aacctcaaga atttctacat
     2701 ggtttattag aaagtatggc tagaatagaa actagaggat atgaactatt acaaaatcta
     2761 ggtgcagatc ctttaactca tatttataca gcaggtggtg gtgctgctaa ttctacttgg
     2821 acggctattc gtcaacgtta tttaaaagta cctattgttt catcaaagaa tacagaggca
     2881 gcttatggaa cggcactttt ggcaatgggg aataaattaa tagaaaaacg aaccacagag
     2941 gacacagagg acgcggagga atgaggaggg aaagaattat ttataagttg taaagttaag
     3001 tttaattcag tattcaattg ttaatttata tggttatgaa tctaatcact aagctagttt
     3061 cccaaatgta taacaatttc aagtttccgc aaatcttggt ctttggtttg attattacca
     3121 gcttgtttgc ctgtggttta gatgtcactg aaaatagtac atctgcaaat gctaaaaatc
     3181 ccgaacagtc acaattaaac caaaatactt tacctaaatc tatttctcaa gcagtgctaa
     3241 gggatgcttc tagagtttct gggataaaaa gttctgattt gcaaattact caagttacat
     3301 ctaccaattt tagtaatccc tgtgctttca aatttgggga agtttgcaca agggaatata
     3361 atcctattcc cggttggatt gtaattgtcc aagtgcaaaa acaatcttgg acttatcatg
     3421 tgaataaatc tggttcacaa attctgttag atccgaaaat tagcagtaat caattaccga
     3481 aaaaaattgc taatgcggtt ttgagtaatg cttctaaacg ttctggttta gcagtaaatt
     3541 ctttgaaaat tacccagtca attcagaaaa cttttagtaa ttcctgcgtt tttaattttg
     3601 gtgaagtctg cacccagaat tttgctccca ttaaaggttg ggaagtaatt gtcaaagtta
     3661 aaagccagtt ttggacttat catgtagata taactggtac acgcctagtt ttagatcctc
     3721 agggtatgga ttaacccgtc gagaacttgc agagtaatat caacaggtaa ccgttcaaag
     3781 gggtaattgg tgacttaaat accgtgaaat agaatgtgtg aagtagttat agctatgaaa
     3841 atcactattt gttagaagca attgcaaatg gattttatca ggatgaaaaa ctagaacaat
     3901 tacccgcagg tttacaagga tattatgaga gtcattggcg aattatgggc atgaccaaca
     3961 aacccttacc caaggataaa attaaaatta tttatgcaat ttgttttgct aaaactgcca
     4021 tatctgatga tttaattgct aaacattcta aacaaagtaa tctgaaagta caggaagtta
     4081 ttcgagattg gacacagttt ttacaaatac aaaaacagga aaataatcaa ccatatcttt
     4141 atcgctttta tcatgaaagt ttccgagatt tcttgcaaaa acaggatatt gtgaaagctg
     4201 cgggggtgac tttatcagaa gttaaaaaag aaatctccga caatatcagt gagggattat
     4261 atgactaaat ttattgattg gtttaatcaa ttatctgaag aaggtaaaaa atattacttg
     4321 ggttgcttgc ctgaaacatt attagatggt aaagactata caaaactgta taaattatta
     4381 tctgactatg aatttatcac tacaaaaatc aatcatcctt tatttggaat acaagcacta
     4441 attgaagatt atgatttatt agataattcc aaagttaggt ataattcaga atatgcggaa
     4501 actgtcaaag cattaaaatt aattcaaggc actttatcca gatcagcaca cataatattt
     4561 caagactcaa aacaactcaa gggacagcta tcagcacgac taacacattt tgatttacca
     4621 gaaattaaaa atttattagc acaaatagcc acagataaaa atataggttt atatagttta
     4681 atgggtagtc tcatacctcc tggtggtggt ggattaattc gcacccttga aggtcatagt
     4741 tactcggtag aagcgatcgc agtcacaccc gatggaaaaa ctgtgatttc tggttctgat
     4801 gacaagacta tcaaaatctg ggatttgaga acaggaacag aaaagtttac cctgactggt
     4861 catagtgact gggtaaatgc gatcgcagta actcccgatg gaaaaactgt gatttccggt
     4921 tcagtagaca agactatcaa aatctgggat ttggtcacag gaacagaaaa gtttactgtt
     4981 aaaggtcata gtgactttgt aaatgcgatc gcagtaactc ccgatggaaa aactgtgatt
     5041 tccggttcag tagacaagac tatcaaaatc tgggatttgg tcacaggaac agaaaagttt
     5101 actcttaaag gtcatagtta ctcggtaaaa gtgatcgtat tcacacccga tggaaaaacc
     5161 gtgatttctg cttcaacaga caacactatc aaaatctggg atgtggaaac aggaacagaa
     5221 aagttcactc ttaaaggtca tagttcctgg gtaagtgcga tcgcactcgc acccgatgga
     5281 aaaactctga tttctgcttc aagagacaac acaatcgaaa tctgggattt agcaaccaga
     5341 aaggaaatag caacttccac cggagaaagt cccataaatt gctgtacagt tgctcccgat
     5401 ggggtgacaa ttgttgcagg agaagaatca ggaaggttaa atttattgtt agagaattta
     5461 tctagcaccc ctccccaacc ctccccgcag ttgcgatgga tatattgcgg gttttaactg
     5521 ctgaagaaac tccagcaatg tcaacggtag ctattagtaa aattatcaaa gctgatgttt
     5581 ttgatgtggc ggaaattatg gaaacttggt tagaatttct gcaagaaata caaacaaata
     5641 agggaactaa atatcaatta tatcatcaca gtttccggtt atatcttcat gattacagca
     5701 ctttgcgtaa catttaaata caataattaa aggaataatt tgcaaaatca agatgaaacc
     5761 tgtctcagtc gcgcaaagtg ctgtatatca ttttagtttt cctggtttat taattgaaag
     5821 ttactaaacc tgacattctg gatttttttg ataatcagga gtagcatcag gataaaaggt
     5881 acgaacacca ccttttttag caacaaaaac attaacaaat gttttgttgt catcagttac
     5941 agtcagcaaa caagctttag atgtagtttc tgaattgggg ttatttttgt atgctttcgc
     6001 tgcaattgtt aaaatctctt ctgcattgag ggtgtaacca tatcctttta gtggatcatt
     6061 cacagtgctt ccaccaactt tcattctcac accaaaggaa taaacagcac ctggtttaac
     6121 ttcagatgta ctcacagact tgggtaaaat tccagcaata cgtttatttt gtaaatccaa
     6181 ataacgtcca gcaaaatgta aaccaccaat tttatttttt tctggttcac cacaaaaaac
     6241 atgatcaaat cctttagcat taaaccaaag attagttaga tcttccagaa attcttgatt
     6301 agaagttctt ttgggaaaaa tataaccccc aagattattt ttaatattgc tgaaaacatc
     6361 gggaaaactg ttaaacatgg ttttaaaact agcgggttta actactgttc ccacttctcc
     6421 acacagttga ttaactgctt tatcaaactt attcaacaca ggagggggag gagttaaatc
     6481 ttgtacacca ccaaatttaa ccttgacagg attctttgta ttgtcaaaga aaggtaaaaa
     6541 ttcctctgca ctcactaaac tgggactaaa tagagtggtg atgaaaaata gttccgctgc
     6601 taaaattaag taccgaaatt catgcaaatc tttacaactt tgaacattga tggttttaga
     6661 caagaggttt aacatcattg aaatctcact agtatttgag tatgattgtg aatacttaca
     6721 cattactaat atgaaactat atagtagtgt caattattct taataaatct taaaattatt
     6781 tgctaattaa aatccatcat ccccccaaag tcttccatca ttttttccag tagttcttcc
     6841 atcatatctt cctgtacaga acggagactt tctggacctg cgaaaaattc tttaatagtg
     6901 atctttttat ctttaaagtc ttgaacaaaa tcacgaattt ccgcaactat cttagtttga
     6961 gattgaatag tttccagcat taattcaata caatcaatcc cttgtttagc tgcttttttg
     7021 taatccgcaa ccattgatcc ttccggggta ttttttgcta accggagttc ctgtttcaac
     7081 ttctcatatt gatttttgag aatttgattg tgttgttcta tatttttaca tctgcgactt
     7141 aaatcatctt cgctattatt atcaattgtt tctccgactt catatttacg ttcaatttcc
     7201 aaaagtcgcg ctaaatctcc atcttggtaa gctttattaa tttccttcat tatttccgtg
     7261 tgagtcattt gggtttcatt atctttaacc ttatcagggt gaaagatttc cgctaaccgc
     7321 aaaaatgtct gtcggatttt tctagattca tctgttctcg ctactgtggg agattctgaa
     7381 tcttgttctg cttcccaaaa ttgacgacga cgttgatgat tttcttgagc atcattatct
     7441 tcaaatagtt catctaattc ttcatcatca tcatcatctt cttcttcttc aataggttta
     7501 taactaataa ttcctcccat ttgtaaactt ctgtacaatg attggatatt tttctgggtt
     7561 tgctttccca tttttcttgt atttaaaatt tcggcaaaca aagcatgaat ttctgcatct
     7621 aattcggcca ttgttttcat attcggacta actcgatgaa atacctctgt cgctaaagag
     7681 cgcatttttt cgacgaagtt atttaattct gtccgctttt tcttaatttg ctttaataga
     7741 gattggtgtt ctttctctaa acccgctaac tggagatgta agtcagaaag agctagggga
     7801 gttactgtag ttgaggatgc tgtttttgtt ttgcgtgcca tagcagatgc aattaatatc
     7861 agggttgata aacgaacaca attaatatta tattttaaat attgatattt atagaagatt
     7921 ttaaattaaa ttaactggtt ataattactt aatttaaaac ctttcacagt caacatttct
     7981 cgcacttgct gactcaacaa agccgctaat tctacctcac caccgttatt tatctcggta
     8041 ttcactaaat caggatgaga ataaatttct actaattccg cttcaatttg tggtatcaaa
     8101 cccagtaaat attcttcgct catgtcacca gtttgtaaca atccataaac cctgtcagca
     8161 aatttaattt gattcgcttt taataaaact tctccataac ggcgtaattg tccaaaaaca
     8221 atagaccaaa ttattttagt gaataaatta cggttatcaa ttttgaggtt tttgcttaat
     8281 tcttctgaag gtagacgaat aaatttaatc tgaaattccg cggcaaattc tgttaaaatg
     8341 ttcaaaataa caggatgtac atgaaggtgc aaatgtccat caacatgagc aagatttaaa
     8401 ccagaatccc gaaatttttc taattgagca gatatttcta accgcaattc tgcacgggta
     8461 gcttgattaa attgataact taatcctgct tgggtgggat tattagaaaa attaccttga
     8521 gagtctacta aattgggaat ttgtgcaggt ggtaaaaccg atttaccgca cactaaaacc
     8581 aggtgtaaac ccactgctaa atgagggtga ttttttgcca gtgcgatcgc ctcttgtgca
     8641 gcatcaccac ttaccattaa actagtgcta gttaaaatgc cttcctcatg ggcttgaata
     8701 atcgcttaca gtttctttcg tttctatcgg caacccgata ataaaagtcc catgtacagt
     8761 aataccaagt ttgtggcaat tcttcatgaa ttctcgcgcc acttctagct taataccttt
     8821 tttgatatta ttgagaactt cttgattacc agattcaaaa cctactaata acaaccttaa
     8881 accgttatcc cgtaaagttt tgagagtatc ataatctaaa ttagcacggg cattgcaact
     8941 ccaagttaaa ttgagtcgct tcatgtgttt actaatagcg atcgctcgat gtttgtcaat
     9001 ggtaaaagta tcatcatcaa acatatactc ccgcaccttg tccccaaaga gaaccttggc
     9061 ttcttccatt tctctaccta cggcatcggg gcttttatga cggtataaat gtccgccaat
     9121 agtttgcggc cacaaacaaa aagtacattt tgctggacaa ccacggccag tataaaaaga
     9181 aatgtaagga tgttgcaaat agccaatgaa atatttacta atatctaaat cacgggcata
     9241 aactggcaac acactgggca tagcatccca gtcatgaatt aaaggacgtt cttccgtttg
     9301 gtggatattt cccaacttat cccggtaact caaacccttg atttcatccc aaggtttccc
     9361 ttctgccaat tctttacagg tataatcgaa ttcattgcgg cagacaaaat caattactgg
     9421 attttcccgt aaagttgcct ctggtaaaac cgcaacgtgc gcccctacaa agccaatttg
     9481 cacattggga ttttgcgctt tgatcgtttc tgcacacttg acatcattag ccaaagaagg
     9541 cgtgctggtg tgcataatca ccaattcgta atccttggca attttcagca catcatccac
     9601 tgtctgccca tgaggaggtg catcaactaa cctactgcca ggaactaaag ccgcaggttg
     9661 tgctaaccaa gtcggatacc aaaaagaagt aatttctcgt tttgcttgat accgcgcccc
     9721 cgcaccacca tcaaaaccat caaaagaagg tggactcaaa aataaagtag ttttcatgaa
     9781 tatttttctt gtcagttgtt agttgttagt tgttagttgt caacttaagc caaccaggga
     9841 atgaattccc tgtctaaaag ctaaactcgg ttaaaaccga ctactttgac ataaatagga
     9901 tagtttttga ttttgagtta accagagtgc attttaacgc actttagctt ttagaccgga
     9961 acttaagttc cgggcggatt tgttccccat accctatttc ctaactagct ttagcattca
    10021 cacccatgag gttttcaatc aaggaaacaa catcactacg gctgagtttt tccttaccac
    10081 tagccaaagc atctagagta ccatgagcta aagccagagg aatttggcaa aattgcaaag
    10141 ccggtccatt tggcaaagag ttcgtatagg catctgctag gatgagattg cgacgggcat
    10201 atttttggag atgatcatta tcccaacctg ctgggtaaaa attgactcca cgagttaaat
    10261 cctcaccatt gtttcgcaga atatttactg cttgcaaacc cctaccaaat cctatggctt
    10321 gagtgcggtt gctttgagtt ccatcatacc aattccataa atctgagagc aacaagccca
    10381 cagcaccagc caccccaaag gtataacggt ctaaatccgc ctctgtttct atttgccaat
    10441 ttctatctga ccaataagcc attctatcag ccattgcggc tgttgcatcc caaattcgcg
    10501 gggcaatgct ttcaggtgct aatattgacc attctctaat gcgtaaactg acttcttgta
    10561 aagaattttc gtaaccttta aatcctacag agaaagcatc cagcggaaaa ccatctactc
    10621 cagcttgcaa tgtgaggcta atattctgca ataggcgttt cttagtctcg ttatccaagt
    10681 ttggatcatc ttcaatttgg tcaatggcac gcatacacaa gtatgctgat gccactgctt
    10741 cttgtaatcc tgatggtaaa atactaattg ggatataaaa agttctacta gtttctttaa
    10801 ggatttgcaa cgcatctcca cgtaaatcca ttttgttcac tcctctcttg atttgcacac
    10861 cacacctaat ttcggacttt tcaataatac tagatttagt attgattaaa tcttaaaaac
    10921 catagacact ctagaattta atctaggaat acatagttta taatgtcgca tttaacacaa
    10981 ttttccagct tatcaaagct tagtacagta tataaggttt tggtaacaat aaccttgtct
    11041 acaaggatag agataagaac agcaaagttc ttaggctgaa gtattgcaga agtgccgata
    11101 gagtcactta ttttaccagg atgtggattt tattgttgag tatatgattt tatgatttaa
    11161 gctgtattgt cctacagatt tttatttgtc agaatcagga tgtccaggat ttgaggattt
    11221 tcaggatgtt attggatgat tgttttttgg aagttgggga tttttcagga tgtttttgaa
    11281 tgattgtttt tgaataattt aaaccatcta taaattacca tcctgttaat cctttaatcc
    11341 tggttatcct gattcagaca aatgcaattt tcatcaccaa acaaaccatc tataaattac
    11401 catcctgtaa atcctttaat cctggttatc ctgattcaga cttaataatt ttctgtaacc
    11461 cttaaatgtt acaacttaca agtctcgacg cgagagaaga acccctcccc tcctccactc
    11521 aaaaaccggg aggtggcctt ctggcgcttt cttgtcaaaa gtaatatctg tgtagtacaa
    11581 ccatcttcct ccttttctcc atcctacctt gtccccgaac ttctcccaga tgtctgagtc
    11641 ttctcgcgtt tttaaacttt gataaatgcg tttttgcaca gaaaaaccaa attttccatc
    11701 actgtatttt acccacaagt ggtcaatggt gcgaaggtct tcacagggaa aattattaat
    11761 actttcaaca tctaaccaac cttccttttc ccgtttcgta accgctaacg ttacccgttt
    11821 tgtttccccg tctgcttcct tccatttccc ttgtgcgagg tagtcgcgca gtttcctata
    11881 gtccatatta gatgtttgta gagacgttcc atggaacgtc tctacattaa tgggttgttg
    11941 aatatcaaaa ttaatgggtt gttgaactac caaacccaaa ttttcccgaa acttcaatac
    12001 cgtcggagtc cggttttccg gttctatttc catccctgct aaaatagcat cattcaccct
    12061 ctgactaatg cgagaattat aatgttttgg tgcgggtaaa ggttcatcat catatttccg
    12121 gttttgtgcg ggtactggac taacttcatc ctctttgttg agtccatccg ccgtgagcag
    12181 ataatataaa gtggcagcta aagcataaac atcggtatga gaaccaaaac tccctttccg
    12241 acgatattgt tcaatgggtg cataaccttc cgttaaagca tttgtcatgc ttttagtagc
    12301 aatactgcga gttaaaccaa aatcaattaa aaccgcttct tgtttatttt gacgtaacag
    12361 aatattttga ggtttaatgt cgcggtgtaa actgcctttg gcggtataat ttacccgtgc
    12421 taggttctaa agctttgtag gtagtcccaa aaccaccgct acccaagggt ttaccttgaa
    12481 tgataaatct accgttatta atttcttgtc ctggttgcca aataatcata cccttgttag
    12541 tagatacggt ggagattaat ttaaatatat cattaccgag atccccgact gcttaaagaa
    12601 gtcggggatc ttgttttgga tgttattgga tgatttttgg tggggagttg gtgattttat
    12661 ttgtctgaat caggatactc aggattaaag gatttacagg atgttattgg atgatttttg
    12721 gtgggaagtt ggtgatttta tttgtcttat taaactctca tattttcaat ttgaaaattg
    12781 attattaaat attaaatgaa tatttaaaat atgacattta aaaccaacat cttgcaaatc
    12841 ctaaaatcct ggatatcctg attctgacaa ttgagagatt catatccccg acttctttaa
    12901 gcagtcgggg atatggagga ttaagccaca acttccactg gtttggcttc tggtaacaac
    12961 cgcttcacac cttgcttgac aaaagcttcc aaaacagcca ttttttgatt ttgctggaat
    13021 acagtttgca aaacttgacg cagtttgtcg agatttaacg cacttcctga ttctaaagca
    13081 atttcttgct gttcaaaata tgctactaaa cttaatcccg ctactcttgt gaggtaagca
    13141 gcactcactc cttctaccat tcccccagcg acaaaggtag caacattagt tttcagaata
    13201 cccgttacag ccctagttga aagttccact aaacctaatt tcaacatcaa acttcccata
    13261 gttcccgcta cttgttgcgc ttgttctaag gaaattttct gctgataaat attacccaaa
    13321 tctatcacca tttgagcagt aatcgccgca gttgcgagaa tatctaaagc aggtacggga
    13381 ttagcaaaag ctgccgccgc tgcaatccat tgatattgtt ctataaaagg tgtggcttga
    13441 tctcttctca caccatttaa acaatttttg gcttcagctt tgagtaataa taccttgcga
    13501 tttgtggtat tacaaactag ctgttctcct tgctgtccca caaccgctgc taattgttgt
    13561 gttaattgct ggatatctgg cgttggttgt tccatccatt cttggaaaga accatcttct
    13621 tcatgtttcc gcactttcac aggtacagga gaagccgcag tcgcaactac atttgcacca
    13681 atgcgctgtt ttaatgattg aaaaactgta gcgcgttcat caggttgata ttgatcttgt
    13741 ttgttgaaaa ccagcaaact tggttgtttt gtggctttta gctgttgtaa aacctgaaat
    13801 tctgaatctg ttaaatcacc gttggtcaga aataagacaa aatcagattt ctgcatttct
    13861 gataaagtga caacatcgga attttcacca gctacggaaa ataaaggcgc tgtttccgca
    13921 aaatgcaaag atattcccgg aatagttggg gcagttttta gaacttcaat aactgtactt
    13981 ttacctacgg atttaccacc agtaacggcc agagtgattt cctttctatc cagttctaag
    14041 ggcaatttag cgagggtttc ccggagaatt gctaggtgag gatgattggc tgcttcttgt
    14101 gccagttgat taattattac ttgggtttta gcgatcgctt tatccacatc tgcccgattg
    14161 acaactatat catttagctg ttcacctaat tggggctgat ttttattgaa caaccacaaa
    14221 cccccaccga cagccaaagc actcaggagt ccgaattctc ccacttgcac tattgaatca
    14281 tgccaactgt ctaacatcca cagggagaac gatagtccta gtcctcccac taaaatcggt
    14341 cgctgtaact tcacaattgt gattctccac gcgaggaaaa agcttttacc tcactttagt
    14401 ccaaaacctg ctactcggat tgaggttggt gaacaaacgt cttttgattt tgaagactcg
    14461 caagcggcta aaagcatttt ttttgctatg tataaacaac acatcaattt ttcttaatta
    14521 attgttgtat ctagtgataa agtcaatgtt aacatattaa cttttcttga aatgacaggt
    14581 agataggata aatagtctgc tcactaatta tacaggtgaa atcagtgaat aaaacagcaa
    14641 gccaattagt cttaactttc ggaatactag cattgacaag cagttttaca aatacgcttg
    14701 gtcttgccca aaatattcaa aaaccaaatg aagcgacaaa agttactaac tctgccactg
    14761 acgcacttat caaagtagat ggttctagta ccgtatatcc cattacccag tccatagcta
    14821 aagcatacca agccaaccta aaaaataata gtcaaataca agtaaatatt tccggtacta
    14881 gcggtgggtt tgaaaaattt tgtactcgta aaatagatat cagtaatgct tctcgaccaa
    14941 ttaaccagtc agaaatggca gcttgcaaga aaaatggcgt aagttacatg gaattaccca
    15001 tagcttttga tgccgtaacc attgctatta acccccaaaa cacttgggca aaagacatta
    15061 cagtagccga attgaaaaaa atctgggaac ctgcggctga aggaaaaatt acaaaatgga
    15121 atcaagtccg agattcttgg ccagaccgcc caattaaatt atatggtcct ggtggtaagt
    15181 ctggaactta tgattatttt accgaagcaa ttgtcggtaa agccaaagcc agtcgcaaag
    15241 attacacagc cagtgaaaat gatgaagttt tagttactgg tgttaataaa gatcctaatg
    15301 ctttgggtta ctttggttat gcctactacc aagaaaacaa agataagtta aaagttttag
    15361 cagttaatag tggtaaaggt gccgttttac catcaccaga aacagtagaa aaagccaaat
    15421 atcaaccact ttctcgacct ctattcatat atgtcaatct tcggtctagt aaaaacaaaa
    15481 ctttggtgta taaccttgcg gatttctaca ttaaaaatgc ggcaaaaata gtcagttctg
    15541 ttggctatgt tcccttatca gaggaagctt acaaacttaa ttctatccac ttgtataaag
    15601 gcaaagccgg aacagtattt ggtggtaaat cggagtttaa cctaagtctt gggcaattgt
    15661 tacgcaaaca aaagcaattt tagtaattaa taaaaatcat gacaattaga gtaggaatca
    15721 atggatttgg gcgcatgggc aggttatccg ttcgtgctgc ttgggattac ccagaaatag
    15781 aatttgtcca cattaatgaa accaaaggtg gaacagaagc cgcagcacat ttactcaaat
    15841 ttgattctgt acatgggcgt tggacaccag aagtagaagc ggtcaaaaac caagttgtta
    15901 ttgatggtaa atccctgagt tttactgaat attcccaacc aggtgaagtt ccttgggaag
    15961 attttggggt tgatatagtt ttggaatgtt ctggtaagtt tagaaccccc gctacccttg
    16021 acccctattt taaaagaggt gtgcagaagg taattgttgc tgcacctgtt aaacaagagg
    16081 ctttaaacat tgtcatgggg gtaaatgatc acctctacca agctgataaa catcatttat
    16141 taacagcagc ttcttgtact actaattgtt tagccccagt cgtaaaagta attcatgaag
    16201 gtttaggaat taaacatgga gtaattacca caattcacga caatactaat actcaaacta
    16261 ttgtagatgc acctcataaa gatttgcgtc gggctagggc tacaagtatg tcattaattc
    16321 ccactagcac aggttcagcc acagctattg ggttaattta tccagaattg aatggtaaat
    16381 taaatggttt agccgtgcgt gtacctttgt taaatgcttc tctcacagat tgtgtatttg
    16441 aagttgaacg ttcgaccaca atagccgaag ttaatgattt attaaaaact gcatctgcac
    16501 aaaaaccgtt aataggaatt ttgggttatg aagaacgtcc tttagtttct attgattata
    16561 aagatgatcc tcgttctgca attattgatg ccctttcgac aatggttatc aacgaaactc
    16621 aggtgaaaat attggcttgg tatgacaatg aatggggtta tgttaaccgc atgactgaat
    16681 tagcacgaaa ggtggcgtta agtttgcaga aataatggtt ttctaggggt gggttttcag
    16741 tcctacccca taaagatttt tgcacgcaga gagaatgagg gtttttagtt tgtatttaca
    16801 attttgaatt tttaatcatg gcttctacta ctgcttctaa ttctaatttc aagaattata
    16861 ttctggtgac actggcatac tggggcttca ctttgactga tggggctttg cgaatgttgg
    16921 ttttgctcta ttttaataaa attggttata cacctattca aattgcctct ctgttcctgt
    16981 tttacgaggt atttggaatt gttacaaact ttctcggcgg ttggattggt tcccagtttg
    17041 ggttaaaagt aactctttat actgggattg gattacaggt gttttcttta attatgctgt
    17101 cttttctgaa tccagggtgg gcgcagtggc tttcagtcct ttatgtgatg gttgcacagg
    17161 cattttcggg aattgctaag gacttaacga agatgagttc taaaagcgcc attcgcttgg
    17221 ttgtgcctca agatgctcaa tcttctttgt ttaagtgggt ggctgtactc acaggttcta
    17281 agaatgcttt gaaaggtgtt ggtttctttc ttggtagcgc ccttctcagt tctgtgggtt
    17341 ttattaattc cctatggatt atggctgggg gactggcttt aattatgttt tctgggttaa
    17401 tgctgcccaa agccatgggt aaaatcaaga aaaaggtcaa atttagccaa cttttctcta
    17461 aaagcgcaga aataaatatt ctctcggcgg cgcgattctt tttatttgga tctagagatg
    17521 tatggtttgt tgtggcttta ccagtgtttt tacgtgggac tttgggctgg tctttttatc
    17581 aagtgggggg atttttagct tgttgggtga ttggttatgg gattattcaa tttttagcac
    17641 caacactcat gcagcgattt ggttctggtc gtccacctca atctcaaact attcgattct
    17701 ggacatttac attaacggct gttcccggtg cgatcgctct agcactacaa ttaggtttac
    17761 ccgctaatat cgtaattatt gctggacttc tcgtttttgg tgtcgtattt gctttcaatt
    17821 ccgctgtcca ttcctatttg gttttagcat ttactgatga tgataaagtc gctttaaatg
    17881 tgggttttta ctatatggca aactccggtg gtcgtcttgt gggtacagtt ttatccggtt
    17941 tggtttatca aatatttggc ttagttggtt gtctgtggac atcaatgttt ttcgtattcg
    18001 cagcaggttt agtttctctg aagttaccta atcctcaacc tagtaaagct atagcttgga
    18061 aaactggaga cggagaataa tatttcccag tctaaagata tgctatgcaa acaggtatat
    18121 cctactgtag tgagatgacc ccaaaattta tttgagttta aatatggtag tgggcaattg
    18181 gtgacagttt ttgcctcttg cctaaatact aatttgtaat aaatacagat tttataatga
    18241 caacaagatc cccgacttct ctaagaagtc ggggatctga aactaacatt tatagaggtt
    18301 aattaagtgg aatatgtaac taaacaaccg actataaaac gcctttcttt tctggatcgc
    18361 tttttgacat tgtggatatt tctggcaatg gcaactggag tgggtttagg ctattttttc
    18421 cccacaatag aagtttttat caatcatttc caaataggaa caaccaacat tcccattgct
    18481 attggtttaa ttttgatgat gtacccaccc ttagcaaagg taaaatatga agaattagga
    18541 gatgtatttc gtaataaaaa aattctcgga ctatcattat cactaacttg gataattgcc
    18601 ccaattgtaa tgtttacact tgctattctt tttttacgcg ataaacctga atatatgact
    18661 gggttaatca tggtgggaat agccccttgt attgctatgg taattgtctg gagtgaatta
    18721 gctaagggag atactgaata tacagccgga ctggttgctt ttaatagtat ttttcaaatt
    18781 ctcttttaca gcgtttatgc ttggtttttt ctggcgattt taccgccttt atttggctta
    18841 caaggaacta ttgtcaatat cagtattggg gaaattgcca acagcgtttt tatctattta
    18901 ggtattcctt ttctggctgg atttatgact cgctttattt tagtaaaagc taaaggtaag
    18961 cagtggtatc atcagcagtt cattcccaaa atcagtccga ttactttaat tgctctttta
    19021 tttacaatca tggtaatgtt tagtttaaag ggcaatttga ttgtgcagat tcctctcgat
    19081 gttgtccgca ttgccattcc cctaattatc tatttcctat tcatgttttt gagtagtttt
    19141 tacctagcat ggaaaattaa agctgactat gccaaagctg ctagtgtggc atttaccgct
    19201 gctggtaata attttgaatt agcaatagct gtagctattt ctgtatttgg gattaaatct
    19261 ggtgtggctt ttgctgctgt gattggacca ttggtagaag tgccagtctt aattagttta
    19321 gtcagtgtat ctttctggat caagaaacat ttttttcgcg catctccacc aataggatta
    19381 taattaaata cgtttgatct actattgatt tatttaaatt taatgaaagt tgctgattta
    19441 attacctggt ttgaagaatg ggcaaatccc gcttggtgcg aaagttggga taattgcggt
    19501 tggcaggttg aacccggtgt tttgtctaca gaagcgcggg ttttagtgtg tttaactccg
    19561 actttagccg taatgcagga agcgatcgct ctccatgcta acctaatatt tgcccatcat
    19621 cccctcattt ttcaccctcc caaatctttc cgcactgggg aagctttaac ggaaatggtg
    19681 cggttagctt ttacccataa tattggaatt tataccgccc atactaattt tgaccaagtg
    19741 gcagatggaa ctgctgacgt tttatcccaa cttctggaac ttcaacaagt atcacctatt
    19801 gttcctaccc aagcaggttt aggttatggg cgtgtaggaa tgctaaaacc cactttgaca
    19861 ttacaagagg taattaataa tattcaaact caattaaatc cgccccattt aattttctcc
    19921 ccaatggctg atttacaaca gcaaatttca cgggtagctg ttttaggtgg ttctggtgca
    19981 ggttatattt cagccgtagt taaaactaat gcccaagttt acttaacgtc tgactgtaaa
    20041 tttcatcaat ttcaagaaag ccgtgatcgt ggtattatat taattgatgc tggacattat
    20101 gccacagaac gcccagcgtg cgatcgctta gtccagaaat ttcaagccct taacttagat
    20161 tgggtcaaat tgagccagca agatgaagat ttccgccagt ttttacccta gttagatcac
    20221 cgtaaactat ccagaccaag taatacttac atcttgcttc cgtaacgccc tggggatttt
    20281 attcaaaaat ttatacaaaa aaaattattc acggttaaaa aattagaaac aatgttatca
    20341 ttataataca gattttaacc ctggcgaaaa tcttttagag atcagccact ggtttgtctg
    20401 tacttggcta gatagtctag ttaaaaggca aaaagctatg ttatgttcag acgattttat
    20461 tgctaaagca gtcctgccag ggataagtaa ctttccctgt ggaaacgcca aatcaaagaa
    20521 aaatgtctgt agatgttaac agttgctata gctgttcaac taatgcggta aactaaagct
    20581 taagacccaa aatttcatct aacactaatg actaaaaata agtggtaaaa ttgagtcact
    20641 gaacttaccg ggttctcacc gcgaaattca cctcctagag gctgatatta tggggatgaa
    20701 acccgaattt gacaggttta actacgttgt ataaaaaggc agaagcacta catgctacac
    20761 cgcaagattt atcaactgtg ttgcgacggg cgggaggtat gtgttttttt gcgggatcag
    20821 caacgctgga t
//
