LOCUS       NZ_CP051206            20933 bp    DNA     circular CON 09-DEC-2024
DEFINITION  Dolichospermum flos-aquae CCAP 1403/13F chromosome, complete genome.
ACCESSION   NZ_CP051206
VERSION     NZ_CP051206.1
KEYWORDS    RefSeq.
SOURCE      Dolichospermum flos-aquae CCAP 1403/13F
  ORGANISM  Dolichospermum flos-aquae CCAP 1403/13F
            Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Dolichospermum.
REFERENCE   1  (bases 1 to 5151752)
  AUTHORS   Wu,V.
  TITLE     Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial
            Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5151752)
  AUTHORS   Fomenkov,A., Anton,B.P. and Roberts,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2020) Genome Biology, NEB, 240 County Rd, Ipswich,
            MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-DEC-2016
            Assembly Method                   :: HGAP v. 2.3.0
            Assembly Name                     :: Afl_HGAP3_4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114.08x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012516395.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:24:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,921
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 66
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 35 of 187
            Pseudo Genes (incomplete)         :: 162 of 187
            Pseudo Genes (internal stop)      :: 14 of 187
            Pseudo Genes (multiple problems)  :: 21 of 187
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:01:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4573675
            Orig. end                         :: 4594608
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051206.1.
            Bacteria and source DNA available from CCAP 1403/13F.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="14"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20933
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10933](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10933)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    510..525
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.09"
                     /tool="antismash"
     gene            632..811
                     /gene="psb34"
                     /locus_tag="HGD76_RS21855"
                     /old_locus_tag="HGD76_21860"
     CDS             632..811
                     /GO_process="GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="psb34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455811.1"
                     /locus_tag="HGD76_RS21855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21860"
                     /product="photosystem II assembly protein Psb34"
                     /protein_id="WP_168697023.1"
                     /transl_table=11
                     /translation="MPYTNEEGGLLNNFAKEPKLYQAEPPTNSQKRNYIILGIAAMLLI
                     GGVIFVAFTVSNVS"
     gene            1025..3184
                     /locus_tag="HGD76_RS21860"
                     /old_locus_tag="HGD76_21865"
     CDS             1025..3184
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083314.1"
                     /locus_tag="HGD76_RS21860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21865"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_168697024.1"
                     /transl_table=11
                     /translation="MYTENRAKTQLNTSVNETVHTENISLNRQVYQRLKLALSLGLRRQ
                     ILFAICDNLHLRNRIAARLHSTLAYPVGKVIYQRANVGDFSTPAYPRLVTLRLNLSDPN
                     PIAQINQWLANYPPPKVGGSTDNPGRPLPMPGFQIVGVEMLTKEPVAVQRLFLNYLRLG
                     EQHLSSQESSHFVESSLLFWVSRPWLSAIQQSAAKFWHCRTGVFVFAGEPTPTIDNRGY
                     PEKFADSYNLGQENLEHLVVNEEEVSQDIKQTNEFDLPLTSTTDLTQIPDKFPVQSPQI
                     TTNQHFPSLSHINQELQGLIKATINADDSDQIQQLLWEIEQLHIQTAKGEELGIAYQNL
                     GNFYRLQIEQGQATVENLMIAILAYQEAVNYDENSPQLPDILNDLGTLYWMLHRIPDNS
                     EEAKIYIQQGVDFYKLALKIITGDTQPETYTRIQNNLGTAYGDLAKFAEPVENWQSAVI
                     AYDEAIRYRQEEIEPLKYAACQNNLGTAYWHLGQYDQPVENLTKAISAYKLAVVHYQQT
                     NDPLKYAMIQNNMGTAYWNLSQYEQPIENLQLAIQVYNEALKYRTSVDVPQSYAATQNN
                     LGIAYWHLANQPQTIKEDQQKFVKLCINAYKEAVNTAHSLSSLPLSFDLWMTHNNLGLA
                     HYYLVINLSFNGDKKMLSQQLEAALENHLQALSGFDRQTENYQTTISYIISTIRAFHNE
                     LGIQGQNLALSKLPGHLLPNVLPKL"
     gene            3215..5071
                     /locus_tag="HGD76_RS21865"
                     /old_locus_tag="HGD76_21870"
     CDS             3215..5071
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083313.1"
                     /locus_tag="HGD76_RS21865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21870"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_168697025.1"
                     /transl_table=11
                     /translation="MKETVLGVRNLQVEFISDSSKVKAIDDISFQLHQGETLGIVGESG
                     SGKSVTALAIMGLLQYPGKVSGGKIFFSRTSGQPLDLLALSPQEMQLYRGGDIAMIFQE
                     PMSSLNPVYTIGFQLQEAIMRHQNVNAIEAKRIAIAGLQEVKLLASDEQIEEQYLDHNL
                     SASGSFKLAQLVKEHKEAMLERYPHELSGGQLQRVMIAMAISCNPSVLIADEPTTALDV
                     TVQATILALLYELQQSRNMAMIFISHDLGLIAEITDQVAVMYKGKIVEYGAAAQIFNNP
                     QHPYTKGLVACRPSLNRRPHKLLTVSDYMRVTEDEFGKVTIQSKEPAQPAETTRDETNA
                     RLANISSQTPLLTVSNLEVGFPVRGVFGSTKRYHKAVNGVSFDVFPGETLGLVGESGCG
                     KTTLGRTLLRLIEPMSGKILFNGQDITHLTGKTLQHLRREMQIIFQNPFSSLNPRIKIG
                     EAIVEPLLIHGVGKSKQQRQARVVELLERVGLSADDMKKYPHQFSGGQRQRVCIARALA
                     LNPKFIICDESVSALDVSVQAQVLNLLKELQEDFQLTYIFISHDLSVVKFLSDRILVMN
                     QGKIVESGTSDSIYLEPKEEYTQKLIAAIPTGSPERIRNRIG"
     gene            complement(<5132..5356)
                     /locus_tag="HGD76_RS21870"
                     /old_locus_tag="HGD76_21875"
                     /pseudo=""
     CDS             complement(<5132..5356)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953123.1"
                     /locus_tag="HGD76_RS21870"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21875"
                     /product="dynamin family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQQKYQGYRELIDILKSTCKVLNLDRNSQLHQDVNYICDYLVNPN
                     FQIAVFGLFNHGKSTLLNAMLGNRTLPIDL"
     gene            complement(5600..5905)
                     /locus_tag="HGD76_RS21875"
                     /old_locus_tag="HGD76_21880"
     CDS             complement(5600..5905)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953121.1"
                     /locus_tag="HGD76_RS21875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21880"
                     /product="hypothetical protein"
                     /protein_id="WP_168697026.1"
                     /transl_table=11
                     /translation="MKSPHEHNQDIQRQLEKRLREMDDQINAIDQPIYKTQKHQPEQSQ
                     KPWLRKIILGGKFFGLAVATIVAVRVASVLAGIIIFGALAFLFYKLFLESQIKNSK"
     gene            6183..7157
                     /locus_tag="HGD76_RS21880"
                     /old_locus_tag="HGD76_21885"
     CDS             6183..7157
                     /EC_number="2.5.1.47"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083311.1"
                     /locus_tag="HGD76_RS21880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21885"
                     /product="cysteine synthase A"
                     /protein_id="WP_168697027.1"
                     /sec_met_domain="PALP (E-value: 1.1e-68, bitscore: 230.0,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDIKNGFVGTIGNTPLIRLNSFSEETGCEILAKAEYLNPGGSVKD
                     RAALYIIEDAEKKGLLKPGGTVVEGTAGNTGIGLAHICNVKGYKCLIIIPNTQSQEKID
                     ALTTLGAEVRPIPAVPYKDPNNYVKLSGRIAAEMENAIWANQFDNLANRIAHYETTGRE
                     IWQQTNGRIDGWVASTGTGGTYAGVAMYLKEQNPGVKCVVADPLGSGLYSYIKTGEIKI
                     AGNSITEGIGNSRITANMEGAPIDDAIQIDDSEALRVVYQLLRKDGLLMGGSTGINVGA
                     AVALAKQLGPGHTIVTILCDSGSRYQSRIFNSAWLASKGLVIN"
     gene            7346..7657
                     /locus_tag="HGD76_RS21885"
                     /old_locus_tag="HGD76_21890"
     CDS             7346..7657
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015215865.1"
                     /locus_tag="HGD76_RS21885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21890"
                     /product="ferredoxin"
                     /protein_id="WP_148763768.1"
                     /transl_table=11
                     /translation="MSNVPTQEQPLPISVKVCQHRTCRKQGAEEVLAALQALPAPNVTI
                     AASGCLGQCGNGPMVLVLPEMVWYCRVLPQEIPRLVEQHLLAGKIVKEMLYYRFHPQG"
     misc_feature    complement(7680..7698)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.54"
                     /tool="antismash"
     gene            7715..8305
                     /locus_tag="HGD76_RS26025"
                     /old_locus_tag="HGD76_21895"
     CDS             7715..8305
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083309.1"
                     /locus_tag="HGD76_RS26025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21895"
                     /product="DUF5331 domain-containing protein"
                     /protein_id="WP_015083309.1"
                     /transl_table=11
                     /translation="MDIQHLRQALKMKWLIYYEQNRSWLVKMRIWKDYAGIRRPSSGYI
                     LATLSTLEPELKKILPFILDLNNDPDRIIAALCLHFNPEQELNLLKSQHSTAKNEIVST
                     SPAHIALTDSPVPKERKQVLLKMTTGISVIAVATPIHHHSSVKLPVGLAREQIDKRLIT
                     TGIPSPNITGISSTYSNIFPSWLDELCPGRGNG"
     gene            complement(8325..9305)
                     /locus_tag="HGD76_RS21895"
                     /old_locus_tag="HGD76_21900"
     CDS             complement(8325..9305)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318464.1"
                     /locus_tag="HGD76_RS21895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21900"
                     /product="folate-binding protein YgfZ"
                     /protein_id="WP_168697028.1"
                     /transl_table=11
                     /translation="MLTSAIDTNDINTIQAVREGVVVRDRSDWGRIRISDDDRLRFLHN
                     QSTNDFQSRKPGQGCDTVMVTSTARTIDLVTAYVLDDAVLLLVSPHRRQELMAWLDRYI
                     FFADKVKLTDATEETATLSLIGPQSHAIVENLGAGDLIGQPDGNHILVDRVIVAVGSGL
                     AAPGYTLILPIAEKQIWWEKILGLGAVELSDRAWEMLRILQGRPATDSELTDDYNPLEV
                     GLWQTVSFSKGCYIGQETIARLNTYKGVKQYLWGIRLSAAAEPGTVITIGEEKVGKLTS
                     YTTTPDGHFGLGYIRSKAGGVGLKVQVGETEGEIIAIPFVSHEYP"
     misc_feature    9328..9345
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 25.17"
                     /tool="antismash"
     tRNA            complement(9459..9546)
                     /anticodon=(pos:complement(4583185..4583187),aa:Ser,
                     seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HGD76_RS21900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HGD76_21905"
                     /product="tRNA-Ser"
     gene            complement(9459..9546)
                     /locus_tag="HGD76_RS21900"
                     /old_locus_tag="HGD76_21905"
     gene            complement(10001..10933)
                     /gene="crtB"
                     /locus_tag="HGD76_RS21905"
                     /old_locus_tag="HGD76_21910"
     CDS             complement(10001..10933)
                     /EC_number="2.5.1.32"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140868.1"
                     /locus_tag="HGD76_RS21905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21910"
                     /product="15-cis-phytoene synthase CrtB"
                     /protein_id="WP_168631877.1"
                     /sec_met_domain="phytoene_synt (E-value: 6.3e-97, bitscore:
                     322.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 3.5e-86,
                     bitscore: 287.3, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQLPDSPPRMKTLVSIDESYNLCRELTAKYAKTFYLGTMLMSPA
                     KRQSVWAIYAWCRRTDELVDGPASAITTPETLELWEKQLESIFAGCPLDNYDVALVDTL
                     QRFPLDIQPFRDMIAGQRMDLYRSRYATFEDLYLYCYRVAGTVGLMSTTIMGIDDNIYT
                     APWHQGQQPYLPIEEAIALGIANQLTNILRDVGEDARRGRIYIPLEDLAKFNYTEEECI
                     RGVVDDRWRSLMRFQIDRARQFYIQADKGISYLSADARWPVWAASMLYEQILEVIERNN
                     YDVFSQRAYVPQWKKLRTLPAAWMRSQVL"
     gene            complement(10917..12356)
                     /gene="pds"
                     /locus_tag="HGD76_RS21910"
                     /old_locus_tag="HGD76_21915"
     CDS             complement(10917..12356)
                     /EC_number="1.3.5.5"
                     /GO_function="GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pds"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083306.1"
                     /locus_tag="HGD76_RS21910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21915"
                     /product="15-cis-phytoene desaturase"
                     /protein_id="WP_015083306.1"
                     /transl_table=11
                     /translation="MRVAIAGAGLAGLSCAKYLTDLGHTPIVLERRDVVGGKVAAWKDA
                     DGDWYETGLHIFFGAYPNMLQLFKELDIEDRLQWKEHTMIFNQPEAPGTYSRFDFPDLP
                     APINGMVAILRNNDMLTWPEKISFGIGLLPAILQGQKYVEEMDKYSFREWLQKQNVPPR
                     VEKEVFIAMSKALNFIDPDEISATVLLTALNRFLQEKNGSKMAFLDGSPTERLCQPIID
                     HITERGGEVRLNAPLKEILLNEDGTVKGFLLRGLNGAEDEIFTADTYVSALPVDPLKPI
                     LPISWKEMPFFQKLEGLEGVPVINVHIWFDRKLTDIDHLLFSRSPLLSVYADMSNTCRE
                     YANPHRSMLELVLAPAKDWISKSDEDIVAATITELEKLFPQHFGGDNPTKMLKYHVVKT
                     PRSVYKATPGRQQYRPSQETPITNFYLTGDYTMQRYLASMEGAVLSGKLTAQAIFQAQT
                     VANPSNLQTPTRPPAKNAATA"
     gene            12911..14140
                     /locus_tag="HGD76_RS24995"
     CDS             12911..14140
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /locus_tag="HGD76_RS24995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="WP_210967665.1"
                     /transl_table=11
                     /translation="MISTGIIWATSFTGGTLVLTANLPLGLIAAFSPIQLPVNLVKPQA
                     QFLIISQEYKTAWQKTLISKTSLTFDWINRRLSSYRDRSMTLPSTTLKAKETKFCASVP
                     ENIPAQLHWQNSTNFRQIIFTQVSVPKLDLSPATVVRSLESFFNIINNPIEPNGSYDYS
                     PVLIVKRNSSSYEVWVNNSFVARLPDQITANALQNRLQQLVISPTEKPTQLQPGLVDKT
                     PSLMIGNRLLFAIDRKVSDELGRSGDILAMEWTNNLRIALHTKPLTLIQGQMEMYGLQS
                     SNTKLSGIASWYGGYFHGRLTANGEIYNQDDFTVAHRSLPFNTFLKVTNLQNNKSVIVR
                     VNDRGPYIAPRSLDLSRTAARCLDSEHTGVVAYQAVVLKQNAPQMTLKPSPPKTENMAN
                     AKSELLVFNF"
     gene            14342..15427
                     /gene="nagZ"
                     /locus_tag="HGD76_RS21920"
                     /old_locus_tag="HGD76_21925"
     CDS             14342..15427
                     /EC_number="3.2.1.52"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083304.1"
                     /locus_tag="HGD76_RS21920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21925"
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="WP_168697029.1"
                     /transl_table=11
                     /translation="MSISQLSQSFGNHLILGISGTSLNDDDKRALNELKPIGVIFFAKN
                     FLDGVPYPVWLEKFKTLIDEVHQYSERDSIFTTLDHEGGRVVRTPLPITRFPYAYLLQS
                     HAYEVAKATALELKSLGINLSWSPVADIFSNPQNPIIGSRAFGNTPQTASEGAREYYRG
                     LREEGILGSAKHFPGHGDTSTDSHLELPILNLSREDLRNRELIPFQTLIQDKIPLIMTA
                     HILFPQIDPEVPATLSKIILNDILRKELGFEGVIVSDDLDMKAVSEIFMQSGTVARAFH
                     AGCDLFIVSRNINSSSLERTYKIAEDFSASLNNGSLDAGVVEAARERVEKLLRITPQYP
                     VSTLDKETLVKNAELAISCCL"
     gene            complement(15464..>15823)
                     /locus_tag="HGD76_RS25000"
                     /pseudo=""
     CDS             complement(15464..>15823)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869213.1"
                     /locus_tag="HGD76_RS25000"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="amino acid ABC transporter permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="AYIAEIVRGGIQSIEIGQSEAAKSLGLNSVQTMTYVIFPQALRRM
                     IPPLGNEFISLLKDTSLVAVIGFEELFRKGQLIVSENYRAFEVYAAVAVIYLCLTLICS
                     QLFSRLEIWMNPMSK"
     misc_feature    16096..16111
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.04"
                     /tool="antismash"
     gene            complement(16295..16786)
                     /locus_tag="HGD76_RS21930"
                     /old_locus_tag="HGD76_21935"
     CDS             complement(16295..16786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994191.1"
                     /locus_tag="HGD76_RS21930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21935"
                     /product="Imm30 family immunity protein"
                     /protein_id="WP_168697031.1"
                     /transl_table=11
                     /translation="MNQNNLLATLQENRLMQTEEQVTNFENALTEIAESPDENKLSAYH
                     LILDDQCQQPEVMFSLIHFLESFDMEKQITAFIHVVPQLMITAPEWTRIIHNRILNDVS
                     ACGVYRHGLELINRKNPHHFIYDLLEESVINQINYHIEAPRGILVGELAEPHSLTRLPS
                     "
     gene            complement(16798..17076)
                     /locus_tag="HGD76_RS21935"
                     /old_locus_tag="HGD76_21940"
     CDS             complement(16798..17076)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994190.1"
                     /locus_tag="HGD76_RS21935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_21940"
                     /product="hypothetical protein"
                     /protein_id="WP_168697032.1"
                     /transl_table=11
                     /translation="MKARVSLNYTTDQGYAIMMEHLSPGKGGRHRQTMSYGKRPNLNLS
                     SREALAQEIWDVRCIYLRQGLYNREIRESLQTLIRQNKYTWPWIFEK"
     misc_feature    18091..18105
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.44"
                     /tool="antismash"
     misc_feature    20620..20634
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.7"
                     /tool="antismash"
ORIGIN
        1 gaattataaa gagaatcaat tatttcatct ttaatagatt taaatataaa aatagattta
       61 ctaattgtac ttttaccact tgcttggggt ccaattaaca gcattatatc cgtaacatca
      121 gcctcaaatt ttttaacagg accaaaattt tctatagaga ttctttgcat aattatgatg
      181 ttttgattga gattcaaaaa tataaatcac tatgaacagt gtaacaaaaa tttgatggca
      241 aatagtcatg taactgaact tcgcgcttaa gaggatgttt gaaaagtggt attccgtaat
      301 tttcatcaca ttgctacccc cctttcccct tgtaaagggg ggaaacaata aaaatccagt
      361 tccctcccct ttacaagggg agggttaggg tggggtaaaa aatatttgat acatcaacca
      421 taacttttca aacaccctct aattaggaat gaattctctt cctgaattga cagtcaggtg
      481 aaatagaata gcacaaaatt aaacataaat tgtctatttt gtcaagacag cagccaaaat
      541 cccagaaact ttgattaatg taactatttt tatgagagtg ctgtaaaatt tatttaacat
      601 taagtaaaga aagataataa ggaatcactt catgccttac acaaacgaag agggtggtct
      661 tctcaataac tttgctaaag aacccaagct ttatcaagct gaaccaccaa caaactccca
      721 aaagcgtaat tatattatct taggaattgc agcgatgctt ttaattggag gggtaatttt
      781 tgtcgctttc actgtctcta atgttagcta attggaaaac tgccctttag tagcctttcc
      841 tgatttcagg ttcttatttt gatcaagagc ctgatttttt gttagaataa aaaaatgcaa
      901 gtataattat cgtctttttt taagattcta gcccacaaaa cttgattcat cctgaaaaag
      961 aaaacatctc tgtgaaaatc caaattataa gatgtgacat taatatttaa tttattcagt
     1021 caggatgtat acagaaaata gggctaaaac tcaactaaat acaagcgtga atgaaactgt
     1081 acatacagaa aacattagtt tgaatcggca agtatatcaa cgcctaaaac ttgctctgag
     1141 tctgggttta cgtagacaaa tcctatttgc tatatgtgat aatttacatt taagaaatcg
     1201 gatagcagcg cggttacact ccactctagc ttatccagtg ggtaaggtca tatatcagcg
     1261 agctaatgtg ggagatttta gcacgccagc ttatccgcga ttagtcactc tgcgtctaaa
     1321 tttaagtgat cctaatccta tagcgcaaat caatcaatgg ttagctaatt atccaccccc
     1381 taaggtggga ggatcaacag ataatcctgg ccgtccctta ccaatgccgg gatttcagat
     1441 tgtcggggta gaaatgctga ctaaagaacc agtagcagta caacgtttat ttttaaatta
     1501 tctccgttta ggtgaacagc atttatcaag tcaggaatct agtcactttg tagaatctag
     1561 tcttttattt tgggtttctc gtccttggtt gtctgcgatt cagcaatcag cagcaaaatt
     1621 ttggcattgt cgaaccggcg tatttgtatt tgcaggagaa ccgacaccaa ctatagataa
     1681 tagaggttat ccagaaaaat ttgcagattc ctataattta ggtcaagaaa atttagagca
     1741 tttagttgtt aatgaagagg aagtttctca agacatcaaa caaacaaatg agtttgattt
     1801 gcctttaact tcaaccacag atttaacaca aataccagat aaatttccgg ttcaatctcc
     1861 tcaaataaca actaatcagc atttcccttc cttatctcat attaaccagg agttgcaagg
     1921 attaattaag gcaacaataa atgctgatga tagtgatcaa attcaacaat tactctggga
     1981 aattgaacag ttacatattc aaacagctaa gggagaagag ttagggatag cttatcagaa
     2041 tcttggtaat ttctatcggt tgcagattga gcaaggacaa gcaactgtgg aaaatttgat
     2101 gatagcaatt ctagcttatc aggaagcagt taattatgat gaaaattctc cacaacttcc
     2161 agatattttg aatgatttgg ggacacttta ctggatgttg caccgcatcc ctgataattc
     2221 tgaagaagca aaaatttata ttcaacaagg ggtagacttt tataaattag cactcaagat
     2281 aattacaggt gatactcagc cagaaactta tacgcggatt caaaataacc tgggtactgc
     2341 ctatggtgac ttagcaaaat ttgctgaacc tgtagaaaat tggcagtccg cagttattgc
     2401 ttatgatgaa gccattcgtt accgacaaga agaaatagaa cctttaaaat atgctgcttg
     2461 tcagaacaat ttaggtacag cttattggca tctagggcag tacgatcaac ctgtagaaaa
     2521 tctcaccaaa gcgatttctg cttataagtt agcagtcgtt cactatcaac aaacaaatga
     2581 tcccctaaaa tatgcgatga ttcaaaataa tatgggtacg gcttattgga atctttccca
     2641 atatgaacaa ccaatagaaa atctccagtt agcaattcag gtttataatg aagccctaaa
     2701 atatcgaaca tctgtggatg ttcctcaatc ctatgctgct actcaaaata atctgggtat
     2761 cgcttactgg catctagcaa atcagccaca aacaataaaa gaagatcaac aaaaattcgt
     2821 aaaattatgt attaatgctt ataaagaagc tgtgaatacg gctcactcac ttagtagtct
     2881 acctttaagt tttgatttat ggatgacaca caataattta ggattagctc attattattt
     2941 agtgataaat ctgtctttta atggggataa aaaaatgctt tctcaacagt tagaagccgc
     3001 tttagaaaat catttacaag cgttaagtgg atttgataga caaacagaaa attatcaaac
     3061 aacaatcagc tatattatta gcacaattcg ggcttttcac aatgagttag gaattcaagg
     3121 acaaaattta gctttatcta agcttccagg acatttattg ccaaatgtct tacctaagtt
     3181 gtaatttggt aattggtaat tggtaattgg taatatgaaa gaaacagttt taggggttcg
     3241 caatctacaa gttgaattta tcagtgacag cagtaaagta aaagctattg atgatattag
     3301 ttttcagcta catcagggtg agactttagg aatagtggga gagtcgggaa gtggaaagtc
     3361 ggttacggct ttagcaatca tgggtttgtt gcaatatcca ggaaaagtga gtggagggaa
     3421 aattttcttt tctcggacaa gtggacaacc gctggattta ttagcattat cacctcaaga
     3481 aatgcagctt tatcgaggtg gtgatattgc gatgattttt caagaaccaa tgagttcttt
     3541 aaatccggtt tatactatcg ggtttcagtt gcaagaagct attatgcgac atcagaatgt
     3601 aaatgcaatt gaagcgaaaa gaattgcgat cgcgggttta caagaagtta aacttttagc
     3661 tagtgatgaa caaattgagg aacagtatct tgatcataat ttatctgcat caggtagttt
     3721 taaattagca cagttggtga aagaacacaa agaagctatg ttggaaagat atccccatga
     3781 gctatctgga ggacaattgc aacgagttat gattgccatg gctatctctt gcaatccatc
     3841 tgtattaatt gctgatgaac caactacagc cttagatgtg actgtgcaag cgacaattct
     3901 agcattgtta tatgaactgc aacaaagtcg caatatggca atgattttta ttagccatga
     3961 tttggggtta attgcggaaa ttactgacca agtagcagtg atgtataaag gtaaaattgt
     4021 cgaatatggt gcagcagcac aaattttcaa taacccccaa catccttata cgaaaggact
     4081 tgtagcttgt cgtcctagtt tgaatcgtcg tccccacaaa ctgctgactg tttctgacta
     4141 catgagggtg acagaagatg aatttggaaa agtgacaatt cagagtaaag aacctgcaca
     4201 accagcagaa actactaggg acgagactaa cgcaagatta gcaaatatta gttcacaaac
     4261 acctctgttg actgttagta acttagaagt gggttttcca gtccgggggg tatttggcag
     4321 cacaaaacgt taccataagg ctgtaaatgg ggtttccttt gatgtttttc cgggagaaac
     4381 cttgggattg gtgggagaat ctggttgcgg taaaactact ttaggtagaa cattgctgcg
     4441 attaatcgaa ccgatgagcg gtaaaatatt atttaatgga caggatatta ctcacttgac
     4501 tgggaaaact ttgcagcact tacgacggga aatgcaaatt attttccaaa atccttttag
     4561 ttccctcaac ccccggatta agattgggga agcgattgta gaaccgctgt taattcatgg
     4621 tgtcggtaag tcgaaacaac agcgacaagc tagggtagtg gaactgttgg aacgggtggg
     4681 tttaagtgca gatgatatga aaaaatatcc tcatcagttt tccggtggtc agcgtcaacg
     4741 ggtttgtatt gctcgcgctt tggctttaaa tcctaagttt attatttgtg atgagtcagt
     4801 ttcagcttta gacgtatctg tgcaagcgca agttctcaat ttattgaaag aattacaaga
     4861 ggattttcaa ctaacttaca tttttatttc ccatgattta agtgtagtca aatttttgag
     4921 cgatcgcatt ttagtgatga atcaagggaa aatagtggaa tcagggacat ctgacagtat
     4981 ttacttagaa ccaaaagaag aatatacaca aaaactaatc gctgcaattc ctacaggtag
     5041 tccagaaagg atcagaaata gaatcggata atgtcagatt tagatccccg acttctccaa
     5101 gaagtcgggg atcttattga tgatgatcat ttaaatcaat gggtaaagtc cgatttccta
     5161 acatcgcatt tagtaaagtt gacttaccat gattaaatag tccaaataca gcaatctgaa
     5221 aattaggatt aactaaataa tcacaaatat aatttacatc ttgatgtagt tgagaatttc
     5281 tatctaaatt caatacttta caggtagatt ttaaaatatc tattaattcc ctataacctt
     5341 gatatttttg ctgcatgatt attatttgtc aattatattt tattaatact cttgtttctc
     5401 cagttccttc tgctttggcg cctctgcgtc tggagcgtga gaaaaaatct gtattgggtt
     5461 tccttgcgtc aacccaacct acttataatt agctgtaata agctaaacaa atagcaattt
     5521 ctaaacgaac ctgagaaacc cgttctaaat catttataaa ttgctcagtt cccacttgat
     5581 aattcatgat gattatactt tatttagagt ttttaatttg cgattctaag aaaagtttgt
     5641 aaaaaagaaa agccaaagcc ccaaaaataa taattccggc taaaactgag gctactcgaa
     5701 ctgcaactat agttgcaaca gccaaaccaa aaaattttcc accaagaatg atttttctta
     5761 gccaaggttt ttgagattgt tctggttgat gtttctgagt tttataaata ggttgatcaa
     5821 tagcattgat ttgatcatcc atttctcgga gtcgcttttc tagctgacgt tgaatgtctt
     5881 gattgtgttc atggggagat ttcattaatg tccaccttgt ttatgaaatc atttagtata
     5941 ttgtatgata aattacgcac agaaacaacc aaagcaatat taacattgct agattgagtt
     6001 taaattcaat cttttgtact tgcttttcag aaattttcgg gaagaagatc ccattagaat
     6061 tgagattata gcaactttaa tgtatttctt ccttagcgtc tttgctcctt agcgtctttg
     6121 cgcgaaacga aattacataa aaatgtcaaa atttgattgg gaaaaactca ggtagatgca
     6181 aaatggatat caaaaatgga tttgttggta caattggtaa caccccatta attcgtttaa
     6241 acagttttag cgaagaaaca ggttgtgaaa tcttagccaa agccgaatat ctgaaccctg
     6301 gtggttctgt caaagatcgg gctgcgctgt atattatcga agatgcagaa aaaaaaggac
     6361 ttctcaaacc cggtggtaca gtagtcgaag gaacagcggg aaatacggga attggactgg
     6421 cacatatttg caacgtcaaa ggttataaat gcttgattat tattcccaat acccaatccc
     6481 aagaaaaaat agatgcttta actaccttgg gggcggaagt tcgtcctatt cctgcggtcc
     6541 cttacaaaga tccgaataat tacgttaaac tatctggtag aattgcggcg gaaatggaaa
     6601 acgccatttg ggctaatcag tttgataatt tagccaatcg tatagcccat tatgagacca
     6661 caggtagaga gatttggcaa cagacaaatg gtagaattga cggctgggtg gcatcaactg
     6721 gtactggtgg tacttatgct ggtgtggcaa tgtacctaaa agaacaaaat ccgggcgtta
     6781 aatgtgttgt tgctgaccct ttaggtagtg gactatatag ctatatcaaa actggtgaaa
     6841 tcaaaatagc aggtaattcg attactgaag gtattggtaa tagtcgcatt accgccaata
     6901 tggaaggcgc acctattgat gatgctatcc aaattgatga ttctgaagcc ttgcgtgttg
     6961 tttatcaact attaagaaaa gatggtttat taatgggtgg ttcaacggga attaatgtcg
     7021 gtgcggctgt ggctttagct aaacagttgg gtccaggaca tactattgta actattttgt
     7081 gtgacagtgg ttctcgctat caatcacgaa tatttaatag tgcatggtta gccagtaaag
     7141 gattagtgat caattagcat ttcttagcga tcgccacata taccaaatat agtatcatag
     7201 tggcgattta gatgaagccc agtgcttggg tgttacttct gattctatta ttaaattatg
     7261 gctggctgaa cgtcttgata aaaaagttac taggaagtaa aaacagtaaa tatttggata
     7321 ttttcagtga attaactgta aacaaatgtc aaacgtccca actcaggaac aacccctgcc
     7381 tatttctgta aaagtatgtc aacatcgtac ttgtagaaaa caaggtgcag aagaagtatt
     7441 agcagcttta caagctttac ctgctcctaa tgtgacaatt gcagctagtg gctgtttagg
     7501 tcaatgtggt aatggtccaa tggtgctagt tttacccgaa atggtttggt attgtcgggt
     7561 tttacctcaa gaaataccca gattagtgga acaacattta ttagctggta aaatagttaa
     7621 agaaatgctt tattatcggt ttcatcccca gggataaaga tcactgaaaa tatataaatg
     7681 ctgtaattta gtacagtaag aagtgaggga atgaatggat attcagcatc tacgtcaagc
     7741 attaaaaatg aagtggctaa tttattatga acaaaatcgt tcttggctag ttaaaatgcg
     7801 aatctggaaa gattatgctg gcatccgcag accttcttct ggttacattt tagcaacttt
     7861 atccacttta gaaccagagt taaaaaaaat cctccctttc attttggatt taaataatga
     7921 tcctgatagg ataattgcag ctttgtgttt gcacttcaat cctgaacagg aattaaactt
     7981 attaaaatcc cagcattcta ccgcaaaaaa tgagattgtg agtacctctc ctgctcatat
     8041 agccttgaca gattcacctg tacccaaaga acgcaaacag gtgttattga aaatgactac
     8101 aggcatttct gtgattgcag tggctacacc gattcatcat cattcttcag ttaagctacc
     8161 tgtcggattg gccagggaac aaatagataa acgattgata actacgggaa ttcccagccc
     8221 aaatattacc gggatttcat ctacttatag caatattttt ccgtcttggc tagatgaatt
     8281 gtgtccaggt agaggaaatg ggtaatggac aattaggcat tacttcatgg atattcgtgg
     8341 gaaacaaagg gaatagcaat tatttctcct tctgtttctc caacttgtac ttttaagcca
     8401 actccaccag ctttacttct gatgtaacct agtccaaaat gaccatcagg agtggttgta
     8461 tagctggtga gtttacctac tttttcttcc ccaatggtga tgactgttcc tggttcagcc
     8521 gcagcactga gacgaattcc ccaaaggtat tgctttacac ctttgtatgt attaagtctg
     8581 gcaatggttt cctgaccgat ataacaacct ttgctaaagg aaactgtctg ccataaaccc
     8641 acttccaagg ggttataatc atccgtgagt tctgaatcgg tcgcgggacg accttgtaat
     8701 attcgtaaca tttcccaagc gcgatcgctc aattccacag cccccaatcc tagtattttc
     8761 tcccaccata tttgtttttc ggcaatcggt aaaatcaatg tatatccagg tgcagccagg
     8821 ccactaccca ctgcaactat cacccgatca actaaaatat gattaccatc tggttgacca
     8881 attaaatcac cagctcctaa attttcgacg atagcatggc tttgaggtcc aatcaggctt
     8941 aatgttgccg tttcctcagt cgcatcagtt aatttaacct tatctgcaaa gaagatatag
     9001 cgatctaacc atgccataag ttcctgacga cggtgagggg aaactaacaa taaaacagca
     9061 tcatctaata cataagcagt cactaaatct atcgtccgag cggtagatgt caccatgaca
     9121 gtatcgcagc cctgaccagg tttgcgagat tgaaaatcat tagtgctttg attatgtaaa
     9181 aagcggagac gatcatcatc agaaatccga atacgccccc aatcagagcg atctctgacc
     9241 acgacccctt cccttaccgc ttgaatagta tttatatcgt tagtatcaat tgcagatgtt
     9301 aacatagtgg tacagagttg ataaaattat caccataagc ggatagagga tgtaggttaa
     9361 catcaagctc tttcaaaact atagtttcca acttggatct agtttatact cagataccgg
     9421 acttctttaa gaaatccggt atgtagcttc tgtaaaaatg gagagggggg gattcgaacc
     9481 cccgaaggga tataatccct taacagatta gcaatctgcc gctttcgacc actcagccac
     9541 ctctccaggt catgagtaat aatcgtagca gaagtttttg agcttgtcaa tagttatttt
     9601 gtaaatagat tcattgacca ttgaccaatg accaagtttt gacactctcc ggtctaaaga
     9661 cgcggagatt ctatagagag tttcagtcta tatccctcag ttatcccagt ttcaggcagg
     9721 gttccttaaa tatttgggtt cttgccctga tttcgtttgc cctggcgggt gaaatcatat
     9781 ctgacaagcg taactttccg agagtccctc ggtagctttt tatgtcttta gtgacaattt
     9841 agttgtatca cgaatatgga ttaaaatttg aaataatttc aaattttcaa attaacgtca
     9901 atgcaggata aaggaaagcc tctagccttc atctcttgtc taaagcccaa atgtggctga
     9961 ctcagtcagc cacattttca agggttttcg acgttcttcc ttataaaact tgagatcgca
    10021 tccaagctgc tggcaaagta cgtaactttt tccattgggg aacataagct cgctgactga
    10081 acacatcata attattgcgc tcaatcacct ctaaaatctg ctcatagagc atggatgcag
    10141 cccaaacagg ccaccgagca tcagcagaaa gataactaat acctttatct gcttgaatat
    10201 agaattgacg ggcgcgatca atttgaaacc gcatgagtga acgccaacgg tcatccacta
    10261 cgccgcgaat acattcttcc tcggtgtaat taaatttggc caaatcttct aggggaatat
    10321 agattctgcc tcgacgggca tcttcaccaa catcacggag gatattcgtg agttgattgg
    10381 caattcccaa agcgatcgct tcttctatag gaagataagg ctgttggcct tgatgccaag
    10441 gggctgtata gatattatca tctataccca taatcgttgt tgacatcaaa cctacagtcc
    10501 ccgctactcg gtagcagtag aggtataaat cctcaaaggt tgcatagcga ctgcgataca
    10561 aatccatccg ctgaccggcg atcatatccc gaaagggttg aatgtctagc ggaaagcgtt
    10621 ggagagtatc tactaacgct acatcataat tatccaatgg gcatcccgca aaaatggatt
    10681 ccagttgctt ttcccatagt tctagggttt ctggcgtggt aatagcagat gcaggaccat
    10741 ccactaattc atctgtgcgg cgacaccagg cataaattgc ccaaacagat tgacgcttgg
    10801 ccggactcat caacattgtc cccaagtaaa aagtcttggc gtacttagct gtgagttccc
    10861 gacaaaggtt gtatgactca tctatagaga ccagcgtttt catgcgcggg ggggaatcag
    10921 gcagttgcag cattcttggc gggcggtcgt gttggcgttt gcaggttaga gggatttgct
    10981 accgtctgtg cttggaatat cgcctgcgct gtcagtttac cagaaagtac agccccttcc
    11041 atgctggcta gatagcgctg catggtgtaa tccccagtca gatagaaatt agtaatgggg
    11101 gtttcctggg aaggacggta ctgttgacga cctggggtag ccttgtaaac tgaacgtggt
    11161 gtttttacca catgatattt cagcattttg gtaggattat cccccccaaa gtgttgggga
    11221 aagagttttt ctaactcagt aatagttgca gcaacaatgt cctcatcgga tttagatatc
    11281 caatcttttg ccggtgctag aactaattcc agcatggaac ggtgaggatt ggcgtattca
    11341 cggcaagtat tactcatatc agcataaacg ctgaggagag gagaacgcga aaatagcaga
    11401 tgatcaatat ctgttaattt gcggtcgaac cagatatgga cattaatcac tgggacccct
    11461 tctaaaccct ctaatttttg gaaaaatggc atttctttcc aagaaatggg caaaatcggc
    11521 tttaaggggt caactggaag ggcagagaca taagtatccg ctgtaaatat ttcatcttct
    11581 gccccattta aaccgcggag gaggaagcct ttcacagttc catcctcatt gagtaaaatc
    11641 tctttcaagg gcgcattcag acggacttca ccaccccgtt cagtaatgtg gtcaataatg
    11701 ggctgacaca atctttctgt gggtgaacca tcaaggaagg ccatttttga gccgtttttc
    11761 tcttggagaa agcgattcaa agccgttaag aggacagtag cagaaatttc atcggggtca
    11821 atgaagttca aggctttgga catcgcaatg aatacttctt tttctaccct gggggggacg
    11881 ttttgttttt gcagccactc ccggaaagaa tatttgtcca tttcttcgac atacttttgc
    11941 ccttggagaa tggcaggtag taatccgatg ccaaaactga ttttctccgg ccaggtcagc
    12001 atatcgttgt ttctgagaat tgctaccatc ccattgatag gtgctggtaa atcggggaaa
    12061 tcaaagcggc tgtaagtccc cggcgcttcg ggctgattga agatcattgt gtgttctttc
    12121 cactgcaacc gatcttcaat atccaattct ttgaataact gcaacatatt gggataagcc
    12181 ccaaagaaaa tatgcagtcc tgtttcgtac cagtccccat cagcatcctt ccacgctgcc
    12241 actttccccc ccactacgtc tcggcgttcc aaaacgatgg gtgtgtgacc tagatccgtg
    12301 agatattttg cacaggaaag tcctgctaga cccgctcccg cgattgctac gcgcatttaa
    12361 acctactatt atttaatatt tcttaactgt tttgtcgttc taattatacg ttgcaatccg
    12421 ttacatttgg cgattctagt ttgattgctt cccaaaaaat tcccaaaatc ttccgccaac
    12481 tatctaattt accctaatca tcagtccagg gattaacaca aatttttttg aagtcaaaaa
    12541 tatttagtgt agtttatgac tcatcatcaa tccgtgtata tttttgggta actgcttgtc
    12601 acaaatacaa aacctgattt ttttattaag tattaatatg caagatgcct atgtgactta
    12661 gtaaagtaaa tggcaggata aatcgctatt cataagcaaa agtaatggaa atattagtaa
    12721 tacctgaaaa ctagatttaa aacttttatt tatttcaatc tctattacgg tatatgatga
    12781 tcccagaggg tttaatcata attaagtctg aaatcacata gaaattatgt aattaaaaaa
    12841 actctctttt tattcaacct gggaattatt tctctaggaa ttgtgcatct atctggagaa
    12901 taaataaact atgatatcaa cgggaataat atgggcgaca tccttcacag gtggtacttt
    12961 ggtgttgaca gcaaatttac cgctgggttt aatagctgca ttttctccaa tacaacttcc
    13021 agtcaactta gtcaagcccc aggctcaatt tctgatcatt tctcaagaat ataaaactgc
    13081 ttggcagaaa accttaatat caaaaacctc tttgacattt gattggataa ataggcgatt
    13141 atcatcatat agagataggt ctatgactct accttcaaca accttgaagg caaaagaaac
    13201 aaaattttgt gcctctgtgc cagaaaatat acccgcacaa cttcactggc aaaattctac
    13261 taactttcga cagataatat ttactcaagt tagtgttcct aaactagatt tgtcaccagc
    13321 tactgttgtg cgatcgctcg aaagcttctt taatatcatc aacaatccaa ttgagccaaa
    13381 tggaagttat gattactcac cagtattgat tgtcaagcgg aattcatcca gttatgaagt
    13441 ctgggttaat aacagctttg ttgcccgact acctgatcaa attacagcta atgctttaca
    13501 aaaccgctta cagcaattag taatatcacc tactgaaaaa cctacgcaat tacaaccagg
    13561 tttagttgat aagactcctt cgctgatgat aggtaatagg ttactatttg caatagatcg
    13621 aaaagtctct gatgaacttg gtcgcagcgg tgatatttta gcaatggaat ggacgaataa
    13681 tttgcgaata gccctccata ctaaaccgct gacactgata caagggcaaa tggaaatgta
    13741 tggattacag tcctcaaata caaaactttc tggaatagcc tcttggtatg gtggctattt
    13801 tcatggtcgc ttaaccgcaa atggtgagat atataatcaa gatgatttca ctgttgccca
    13861 tcgtagccta ccttttaata ccttcttaaa agtaacaaat ttacaaaaca ataaatccgt
    13921 gattgtgcgt gtaaatgatc gcggtccgta tattgcacca agaagcctag atttgtctcg
    13981 aacagcggct cgttgtctcg atagtgaaca tactggagtc gtagcttatc aggctgtggt
    14041 tttaaaacaa aatgcgccac aaatgacttt gaaaccatct ccaccaaaaa ctgaaaatat
    14101 ggctaatgct aaaagtgagt tattagtttt taacttttag taacctggaa ggataaagta
    14161 gagaaaatgc gttgcataat ggcagtatga attctgtttc acgcaaagag gcaaaggagc
    14221 aaagacgcaa aggtaagagt tttaattgtg tagcgtaagc tataagttca atcaagagaa
    14281 tttcatacct gaatttagca acaccagaaa atactttatc ctttacttaa atcaccaaat
    14341 tatgtcaata tctcaattat cacaaagctt tggtaatcac ctaattctgg gtatttctgg
    14401 gacaagttta aatgatgatg ataaacgggc gttaaatgaa ttaaaaccga ttggtgttat
    14461 cttttttgct aaaaactttc ttgacggtgt accatatcca gtctggttag aaaagtttaa
    14521 aaccctcatt gacgaagttc accaatattc tgaacgcgac tccattttta ctactttaga
    14581 tcatgaaggt gggcgagttg tgcggacacc tttaccaatt actcgctttc cttacgctta
    14641 tttattgcaa tctcatgctt atgaagttgc caaagccaca gctttagaat taaaatctct
    14701 gggaattaat ctttcttggt ctccggttgc ggatattttc tctaatcccc aaaacccaat
    14761 tattggaagt cgtgcatttg gaaatacacc gcaaaccgct agtgaaggtg cgcgggaata
    14821 ttaccgagga ttgcgggaag agggcatttt aggttctgct aaacactttc ctggccatgg
    14881 agatactagc actgattccc atttggagtt accaatatta aatttgagtc gggaagattt
    14941 gcgaaaccgg gaattgattc cttttcaaac attaattcag gacaaaattc cgttaattat
    15001 gacggcgcat attttgtttc cgcaaattga tccagaagtg ccagcaacct tatctaaaat
    15061 cattttaaat gatattttgc ggaaagagtt aggttttgaa ggtgtgatag tttccgatga
    15121 tttagacatg aaagctgtct cggaaatttt tatgcaatcg ggaacagttg cgcgggcttt
    15181 tcatgctggt tgtgatttgt ttatagtttc tcgtaatatc aattcttctt ctttagaacg
    15241 aacatataaa atagctgaag atttttctgc tagtttgaat aatggtagtt tagatgcagg
    15301 agttgtggaa gctgcacggg agagggtgga aaagttattg cggataactc ctcaatatcc
    15361 ggtttcgact cttgataagg agaccttggt gaagaatgcg gaattagcta ttagttgttg
    15421 tttgtaaggg aattgaataa aaatcaggag tgtgggtgat agcctactta ctcattggat
    15481 tcatccaaat ttccaacctg ctaaaaagtt gggaacaaat caaagttaaa cacaaataaa
    15541 ttacagccac agccgcataa acttcaaaag cgcgataatt ttccgaaaca attaattgtc
    15601 cttttctaaa taattcctca aacccaatta cagcaactaa actagtatct ttcaataaac
    15661 taataaactc attacctaaa ggtggtatca tgcggcgtaa tgcttgagga aaaatcacat
    15721 aagtcatagt ttgcacagaa tttaaaccga gtgatttggc tgcttctgat tgtccaattt
    15781 caatagattg aattcctcca cgcacaattt ccgcaatata agcgaactca aaaggattat
    15841 ctgcccaaca gggttcgggt actaaccaaa ccaatttgct tttaatctcg tcttcaattt
    15901 catcgctatc gtctctatca aatagcgaag ttaaagcttc ccaatcgcgt tctcttaagg
    15961 agttcaaaga ggaaacattt acctgtagat tattagagta attcacaagc aggtttttta
    16021 gttgactttt acaaaaagta agcatcattg cttaaagact aatatttact tgattttact
    16081 aaatattaca gcaatattga taattattct aacattcatc attctagaga caggaagatt
    16141 tttagttcaa agagaccact taaacagaac tagaaaagct gttagagagt taggttttta
    16201 cacaagttga ttaccttttc tggttaaaaa tagtataaca ccaccagtcc ccaggtgata
    16261 aatcctttat agagttttcc cttcgagtac atcttcaaga aggaagtctt gtcaaagaat
    16321 gaggttctgc tagttctcct actaaaatac cacgaggtgc ttctatatga taatttattt
    16381 gattaataac actttcttct aacagatcat agataaaatg atgagggttt ttacgattta
    16441 ttaattctaa accgtgacga taaactccgc acgctgatac atcatttaaa atccggttgt
    16501 gaataattct cgtccattct ggtgctgtta tcattaattg aggcacaaca tgaataaatg
    16561 ctgtaatttg tttttccata tcaaaagatt ccagaaaatg aattaagcta aacatcactt
    16621 ctggctgttg acattgatca tctaaaatta gatgataggc agataattta ttttcatcag
    16681 gactttcagc tatttcagtt aaagcatttt caaaatttgt aacttgttct tctgtttgca
    16741 ttaagcgatt ttcttgcaag gttgccaata aattattttg attcattgtt ttgtccctta
    16801 tttctcaaaa atccatggcc aagtatattt attctgtctg attaatgttt gcaaactttc
    16861 cctgatttct ctattataca aaccttgacg aagataaata caacgtacat cccaaatttc
    16921 ttgtgctaat gcttctctgg acgataggtt taaattaggg cgtttaccgt aagacattgt
    16981 ttgccgatga cgacctcctt ttcctggtga taaatgttcc atcataatgg catatccttg
    17041 atctgttgta taattaagcg aaactctggc tttcataaaa gcatccgcag gtatatgatg
    17101 aggtgtgagg ttatcacctc tacgtccctg tttgattaag tctccataag tgcctacaat
    17161 tccttctcct ggaagtagtt gaggttgatt attaataatc tatttactcc tgtttggcta
    17221 tttctattgt ttagtatttt gattataaag gaaatcgaat aataagtaaa ttgtctgata
    17281 gcgtagcgtg gcgcaagcca tatcaggata tccaccgatt agaggattaa caggatgttt
    17341 ttaggaaata taggtttttg tgaatgatag aaaatatatt ttcctctgaa tcataattta
    17401 taatcattaa atattcacag aaaatctaca aactacaatc ctgtacatcc ttaagtcctg
    17461 gattagagga ttaacaggat gaagaaatag ataatttagc ttgatatcat ccatgaatat
    17521 aaatattttg ttgcaaaagt ttatcctata tattacaaat atttacaatc taaatgattt
    17581 tcatgggtgt atcatttcaa tatctatgct aaaatgaaca atgattaaat aataaaaata
    17641 aagtaaatac ttatgtctag ttttgagcag tttcaaaatg atttcttcaa tattttcaat
    17701 aatcagaact tctatgaagg caaaacttat ttagaatata ttgccgaacc acaaactaat
    17761 gatgaagata acattgttga tactaaaata gttttgccat tgctgcttgc tttaggtttt
    17821 gattcaggag atatagctaa aaatacgaca ggtaatgcta aagatagtag ccgtccagat
    17881 tttgaagtaa aattagcaag tggaaatatt cgatgttttc tagttgagga taaaaatact
    17941 gcatataatc ttaaaagatc tgatccatta caacaattac gtggttatgc aacgagtcga
    18001 ggttatgaac tgggattaat ttgtaatggt aagttgctat taggttatga tttttctaac
    18061 ccaagttatc cgaatcctgt tttacatctt gatattcaga aaattataga aacttatcat
    18121 tcaactggaa ttgaaaattt aacaattgaa caaaatcaag acttaaaaag tttatatcgt
    18181 agatttaatc agcaaagttt tgaaaatatt gagaatttca ttcaagactt gagtaaacct
    18241 gaaaatgaat ggttgaattg tgcaaaatct caagataata ctcctaactt tgatgaatta
    18301 ttaattgcag acctcaaaca agctattaat cttttagaag aagatgtttt atatcaattg
    18361 caattatcac tggaagaata tgatcaatat tgtcaagcta aatatttacc caatagtatt
    18421 gataaaataa atatatcaga aaatgaaaca ataactaaaa ctttagaagg tttacggaat
    18481 aaaattaaaa atcatctaag agtgtatgga gttctagaag ttggagaatt ttctgatgct
    18541 ggtgaaaaat taattgaatt tgccgaaaaa ccacaaggtt cgattcacga ttttgaacaa
    18601 atatttttag ataaattaag aaatgctcaa gccagaaaac aagctcaaaa agataaaaaa
    18661 cctagtaaaa atcaaaaggt agtgcaatta gatttaattc ctaatatttc cgaatcaaaa
    18721 caagaagaac taggaattaa aattgctgat gaaaataaaa ttaaaaaact tgaccacact
    18781 aagctaagtg aaattttaaa agaatattat agaattgttt tggattggaa agcatgggaa
    18841 gccaatcaaa atttaactca tgcaaatgca attaaaatac atcaatattt tatatcttgg
    18901 cgtaatttag ttagtaaaac cattttacaa ggtgcggatg aagataaatt aaaaacagaa
    18961 tttgctcgac aaactgctta tgtttatgtc actcgtcttt taattgtcag aatttgtgaa
    19021 gacaagaaat taattaatcg taaattctct aatggagggt ttaaatattg gaaagaagaa
    19081 gttgaaccgc gatatttaga tttagcgcaa ggaatgtcaa tggattatct tttagaaatg
    19141 tcctatcgtt ctgctcaaaa tatctatact cacttcttta ataatgctga tttatttaat
    19201 tggtatcgga taaattccaa tactttaatt aaagttttgc acatcctgaa tgggtttaac
    19261 ttacaacaaa ttgatagtga tattattggg atggtttatg gtagatatgt tgaagaagga
    19321 aaacatgaac agggtagata ttttacacca aaaccagtag ttgaatatat tctagactgc
    19381 ataggatata aatctgataa tcctgatatt cgtgacaaaa agttattaga ccccgcagga
    19441 ggttctggga gctttttagt tcatgctgcg cgtcgtttga ttgattccta tcgttcacgg
    19501 agaacaaaca caattcccgt tgaaaatgtt cctgctatta ttcaacaggt gaaaaattgc
    19561 ctattttgtt tagatattaa tccgtttgct tgttatttag ccgaaacaaa tttattaatt
    19621 caagtgattg atttactaaa acaagcgaaa gaagcagata aattaaaaga ctgtactatt
    19681 gatagattta atgtttataa tacagattct cttttattag caaaatcgca agaaattaga
    19741 actacattac tcaaccctat tcttgattta gaactttcta cggttgctca aatcaaaaca
    19801 caaacaggaa tgtttgcaga tggttttgat tttgtagttg ctaatcctcc ttatgttaaa
    19861 gctgatgaac ctggagcaga tatttatcgt cgagaaatcg aaagttctgg acaatttgaa
    19921 acacttcatg aaaagtggga tctttttgtt ccatttattg aactttcttg taaagctact
    19981 aaggatttag gtaaagttgg tttgattgtt tctaggggta ttcaaactaa taattatgga
    20041 gagttactcc gagaatatat tgctaaaaat ctcactatta atcaagtaga tttttttaat
    20101 aatgtgcgaa ttttctctga tgctgttgtt aataatacgg ttcttttttt agaaaagatt
    20161 tcaccaggag atgatcatca ggtaaaaaga gttttacaca ctggttcatt tacacaaatt
    20221 caagaaatta atcctttgaa tcaatctgaa tatcaaggaa aagtatttag acagtttgta
    20281 gttaatgata attttgaaga tgttacatat ttagaagata tttgttattg tactgtagga
    20341 atggttttga atgctgatga aaccaaagct aaaggagcat ttaaaaaaga tattctttta
    20401 agttcacaag tagataaaat tcattctaag aaatatattg atggagaaaa tattattaga
    20461 gaactagcaa ttgataatat tcgttatctg gaatgggata ctgaaagagt tcctggacaa
    20521 attcgtagag caacaattcc tgaattatat aattatccta aaattttatt tggaatgact
    20581 tcatttccca cctatgatcg aggaatagca gaaggagatg gattttacgt tcctgatagc
    20641 gtgagaattt gtgtaagatg ggatgatgtt tataaagtta agcgtttaga gaaagaaaaa
    20701 agacagatgt atgagctttc taaaaagcgt caaaaactta ttggtgatgg aaaaggtaag
    20761 aaattacaaa tttatcaata tgcagaagag aaaataaaaa tagcagaaaa atttgattta
    20821 agatatattg ctgctattct tgcatctagt tttggtaaac gctttttact attaaacaat
    20881 cgtgatgaaa atattatgag tgtcaatagt gaaactcaaa tagctaaaag tcg
//
