LOCUS       NZ_CP051206            22099 bp    DNA     circular CON 09-DEC-2024
DEFINITION  Dolichospermum flos-aquae CCAP 1403/13F chromosome, complete genome.
ACCESSION   NZ_CP051206
VERSION     NZ_CP051206.1
KEYWORDS    RefSeq.
SOURCE      Dolichospermum flos-aquae CCAP 1403/13F
  ORGANISM  Dolichospermum flos-aquae CCAP 1403/13F
            Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Dolichospermum.
REFERENCE   1  (bases 1 to 5151752)
  AUTHORS   Wu,V.
  TITLE     Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial
            Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5151752)
  AUTHORS   Fomenkov,A., Anton,B.P. and Roberts,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2020) Genome Biology, NEB, 240 County Rd, Ipswich,
            MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-DEC-2016
            Assembly Method                   :: HGAP v. 2.3.0
            Assembly Name                     :: Afl_HGAP3_4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114.08x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012516395.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:24:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,921
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 66
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 35 of 187
            Pseudo Genes (incomplete)         :: 162 of 187
            Pseudo Genes (internal stop)      :: 14 of 187
            Pseudo Genes (multiple problems)  :: 21 of 187
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:01:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4350670
            Orig. end                         :: 4372769
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051206.1.
            Bacteria and source DNA available from CCAP 1403/13F.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22099
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="azole-containing-RiPP"
                     /region_number="13"
                     /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or
                     PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or
                     thiostrepton or TIGR03602)"
                     /tool="antismash"
     cand_cluster    1..22099
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((YcaO or TIGR03604) and (((thio_amide
                     and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /kind="single"
                     /product="azole-containing-RiPP"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22099
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12099](-)"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12099)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
     misc_feature    complement(177..188)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.15"
                     /tool="antismash"
     misc_feature    516..531
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.76"
                     /tool="antismash"
     misc_feature    complement(519..534)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.64"
                     /tool="antismash"
     gene            664..2301
                     /locus_tag="HGD76_RS20790"
                     /old_locus_tag="HGD76_20795"
     CDS             664..2301
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278824.1"
                     /locus_tag="HGD76_RS20790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20795"
                     /product="IS1634 family transposase"
                     /protein_id="WP_168697410.1"
                     /transl_table=11
                     /translation="MEIQNIDHLGIVAGIIDSIGIVEIINELIGVEKDEKVNAGQVVKA
                     MIINGLGFVSKPLYMFPKYFETIACEHLIGAGVKPEYLNDDKLGRVMDKLFIKGLDTIF
                     FIIALKAAQKFGVSLSTSHLDSSSMHVHGQYNTSLPFVIFESQKVGNNQELEELAVKSP
                     KEITITYGYSRDHRPDLKQFIIEMICSGDGDIPIFLKLASGNQADSSCFGKIAVEYQKQ
                     LEVNSLMVADAALYTESNLKMMSELRWLCRVPLSIKAAKSLISTLAESEFIDSTIPGYK
                     LASKIQNYAGIEQRWLVVQSQERKESDLHKLTQKITKAESKAVQDLKKLSQERLACVAD
                     AIKALSKLSKQFKYHQIHESTVTQVKSNKKDTSGEISYQISATVSQDESKINTELLSAG
                     RFIIATNVLDSKELSNDSMLREYKAQQSCERGFGFLKDPLFFADSIFLKSPERIESLGM
                     IMGLCLLVYTLAQRHIRNALLESKSTIKNQLGKATNRPTLRWIFQCFQCIHLVTLNQEK
                     HISNWNKDRDFILSLLPDDCLRYYQLVS"
     gene            complement(2782..3627)
                     /gene="folP"
                     /locus_tag="HGD76_RS20795"
                     /old_locus_tag="HGD76_20800"
     CDS             complement(2782..3627)
                     /EC_number="2.5.1.15"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080066.1"
                     /locus_tag="HGD76_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20800"
                     /product="dihydropteroate synthase"
                     /protein_id="WP_168696899.1"
                     /transl_table=11
                     /translation="MSNNLTIRDHCFTWGKRTYLMGILNVTPDSFSDGGKFNTTSAALT
                     QAQAMVSAGADIIDIGGQSTRPGAEQISLEAELERVLSVLELLRPVIDIPISIDTTRSE
                     VAKAAIIAGADIINDISAGTFDPQMLPTVASLNVPIVLMHIQGTPQTMQKCTDYEDLIA
                     DIYNFLSEQITKATLLGIDKNKIIIDPGIGFAKNHEQNLEIFRRLESLKTLNSPILVGA
                     SRKSFIGNILHQPDPKLRVWGTAAACCAAIFNGADILRVHDVQEMQEVTLVADAIYREG
                     "
     gene            complement(3675..4400)
                     /gene="tpiA"
                     /locus_tag="HGD76_RS20800"
                     /old_locus_tag="HGD76_20805"
     CDS             complement(3675..4400)
                     /EC_number="5.3.1.1"
                     /GO_function="GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tpiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080065.1"
                     /locus_tag="HGD76_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20805"
                     /product="triose-phosphate isomerase"
                     /protein_id="WP_015080065.1"
                     /transl_table=11
                     /translation="MRKIVIAGNWKMFKTQAESAEFLSGFLPHLEETPSEREVVLCPPF
                     TDLSLLSKSLHGSRVNLGAQNVHWEEHGAYTGEIAAPMLTEIGVRFVIVGHSERRQFFG
                     ETDVTVNLRLKAAQKHGLTPILCVGETKQQRDAGETESIISNQLKKDLVDIDQTHLIIA
                     YEPIWAIGTGDTCESKEANRVIGLIRSQLTNPLVPIQYGGSVNPNNIDEIMAQPEIDGA
                     LVGGASLKADSFARIVNYQ"
     gene            complement(4632..5294)
                     /gene="nblB"
                     /locus_tag="HGD76_RS20805"
                     /old_locus_tag="HGD76_20810"
     CDS             complement(4632..5294)
                     /codon_start=1
                     /gene="nblB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080064.1"
                     /locus_tag="HGD76_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20810"
                     /product="phycobilisome degradation protein NblB"
                     /protein_id="WP_015080064.1"
                     /transl_table=11
                     /translation="MSVSADSIRELLRSDNLGDRLRAVNQIRELEPQIGLELVQIAIQD
                     KSARVRYSAVSQMDTLGTQDLPLSLNILRGLLHDPEADVQAAAADCLGALKLHDAFEDL
                     QQLYQETPEWLVQFSIIATLGELGDPRAFELLTQALASDNGLIQTAAISSFGELGDPTA
                     VPLLVPYSTNPDWQVRYRVVQALTRLGSDEAKSILATLVNDEVEAIAKEAKAGLQLV"
     gene            complement(5434..5892)
                     /locus_tag="HGD76_RS20810"
                     /old_locus_tag="HGD76_20815"
     CDS             complement(5434..5892)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080063.1"
                     /locus_tag="HGD76_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20815"
                     /product="CBS domain-containing protein"
                     /protein_id="WP_168696900.1"
                     /transl_table=11
                     /translation="MSKTVADVMSRDPILVHPQTPLKEAIQILAEKHISGLPVIDDAGK
                     LVGIISETDLMWQETGITPPAYIMILDSVIYLQNPATYERDLHKALGQTVGEVMSKNPL
                     TISPDKPLREAAKLIQDHKVHRLPVLDNAGKVIGILTRGDIIRTMAAE"
     gene            complement(5983..6132)
                     /locus_tag="HGD76_RS20815"
                     /old_locus_tag="HGD76_20820"
     CDS             complement(5983..6132)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_20820"
                     /product="hypothetical protein"
                     /protein_id="WP_168696901.1"
                     /transl_table=11
                     /translation="MVKKVGQHKISKKSASLVEWLKLIEKLGFYGTMALLKMLFQQKQR
                     LFLK"
     gene            6226..6522
                     /locus_tag="HGD76_RS20820"
                     /old_locus_tag="HGD76_20825"
     CDS             6226..6522
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS20820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_20825"
                     /product="hypothetical protein"
                     /protein_id="WP_168696902.1"
                     /transl_table=11
                     /translation="MRTTPTNNINGTSSNQIEGIIGVIHSFRSPDQKNAETNRQLIDIQ
                     KQRSESEIASNYIKAIADACQAKDSIRSELSAKGLARIWNVDYKTLLHPSCGS"
     gene            6681..6842
                     /locus_tag="HGD76_RS20825"
                     /old_locus_tag="HGD76_20830"
     CDS             6681..6842
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HGD76_RS20825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HGD76_20830"
                     /product="hypothetical protein"
                     /protein_id="WP_168696903.1"
                     /transl_table=11
                     /translation="MANEKSPDIQNTNNADNKEHKIECSSCQCKGRIQPYGSRRLSKCQ
                     KCQGTGFC"
     gene            complement(6920..7342)
                     /gene="mutT"
                     /locus_tag="HGD76_RS20830"
                     /old_locus_tag="HGD76_20835"
     CDS             complement(6920..7342)
                     /EC_number="3.6.1.55"
                     /GO_function="GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="mutT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080059.1"
                     /locus_tag="HGD76_RS20830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20835"
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="WP_168696904.1"
                     /transl_table=11
                     /translation="MNESSSSIPHKIIGVAVIWNDQKQILIDRRLPTGTMGGLWEFPGG
                     KIEIGETIQECIKREISEELGIEIVVGEHLITIDHTYAHLRVTLIVHHSQHLAGVPQAL
                     ECDEIRWVSLDELDNFTFPEANREIIAALRKYHQEN"
     misc_feature    complement(7594..7607)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.9"
                     /tool="antismash"
     gene            complement(7694..9709)
                     /locus_tag="HGD76_RS20835"
                     /old_locus_tag="HGD76_20840"
     CDS             complement(7694..9709)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080058.1"
                     /locus_tag="HGD76_RS20835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20840"
                     /product="family 10 glycosylhydrolase"
                     /protein_id="WP_168696905.1"
                     /transl_table=11
                     /translation="MVSSSIRFSDIQGHWSQGFIEALAARRILSGYSDGTYRPNNPVTR
                     AEFAAIIAAIFSLPVKREYVPFVDVPQTHWAINAIKKVYETGFLTGYPNRRFGIDEGIA
                     RGDALVAMVNGLELAGQVDLDLVSKLAEIYQDGSLIPYYGINQVALATRAGWVVSYPNK
                     KILNYKTAATRAEVAVMVYQALVYLGKAEKIPTDYIVVPPTIPKPTTAHTVKLNHRREF
                     RGAWVASVWNSDWPSRPGLAVEQQKAELLAIIKQLQALNFNALVLQVRPEGDALYASEL
                     EPWSAWITGTQGKAPSPFYDPLEFAIAECHQRNIELHAWFNPYRARANSRVSTVRPHIA
                     VTNPEVVYQWGTQQWMDPGAKIVQDRAYNVIIDVVRRYDLDAIHLDDYFYPYPISGQPF
                     PDHKTYAAYQTSGGKLNIEDWRRENVNQMVLRLSQGIKATKPHVKFGISPFGIYRSGEP
                     AGIVGLDAYSVLYADSKKWLQQGWIDYIAPQLYWRTDQTKQAYQTLLQWWTEINTKQRH
                     IYAGNNLGQLDGKEWKNSEIAKQIQISRNLAGNLSLGNIFFSMTGIQENRQGIADQFKT
                     YYPTPALIPTMSWQSSITPPPPKNLKFMDGKLNWEAGDDQLVRSWTLYLQNSNNWLIQR
                     ILSAGTTFATVLPGTYAVCAVDRLGNESAGMMITVT"
     gene            complement(10001..10729)
                     /locus_tag="HGD76_RS20840"
                     /old_locus_tag="HGD76_20845"
     CDS             complement(10001..10729)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080057.1"
                     /locus_tag="HGD76_RS20840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20845"
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="WP_168696906.1"
                     /sec_met_domain="PF00881 (E-value: 1.4e-23, bitscore: 82.0,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTANEPLELALLYHLNSPVPKSYVKRVPATELRYLPEAPFLELP
                     KAPRDNPLSELLARRTSVRSFERQVMPLVALAQLLDSGCGLNGLREVDGQCYEARNSPS
                     AGGLYPLEVFVSTQAVAGLVSGLYHYEPRGHGLHWVNDAVPTDFVEPLLQQDYILNANA
                     LFVFTSVFMRSMGKYGARGYRFALLEAGHQAENICLMAGQLGLDSLCIGGFDDMSLNAM
                     LGIDGERHAALYCVAVGRKG"
     gene            complement(10789..12099)
                     /locus_tag="HGD76_RS20845"
                     /old_locus_tag="HGD76_20850"
     CDS             complement(10789..12099)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080056.1"
                     /locus_tag="HGD76_RS20845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20850"
                     /product="YcaO-like family protein"
                     /protein_id="WP_168696907.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-73, bitscore: 245.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 1.4e-93, bitscore:
                     311.9, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTAIARPRWQDLVSPHTGVIRSLDRFTKPYTEFDFPVLWQAELA
                     NFQLRKQPDDLRYGVGRGMTDEQAIFGAVGEAIERYCGGIVDYRQIIVSSYEELGNRAV
                     PSTVFSPFSNQQYSHPTFPFPAFNSTTQTSWMNALSLTSKQQVLVPAFLVYLDWGGDQP
                     GDYILPVSSNGMASGPSWEFAAYRGLCELIERDAFIITWLNRLPAPRIYFDHLPGIETE
                     IARHYARFGIELVVFELTTDIQVPVFMAMLIDRSGKGPAVSTGMGCHLDAPTAFHKAVF
                     EVCQARFGDIERMKTGAGANLHQYSDIQNLDDHNAFFYTTARLGELDFLFHHDQSCQVE
                     YLPTYESESEAENLQSVVAKLNSVGVEPYVVEITAPDIASLGFRVVRTLASEIVPIYFG
                     YGQEPLGTRRLFEVPERLGYGGRRTKNDLNPCPHPMA"
     gene            complement(12111..13289)
                     /locus_tag="HGD76_RS20850"
                     /old_locus_tag="HGD76_20855"
     CDS             complement(12111..13289)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137446.1"
                     /locus_tag="HGD76_RS20850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20855"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_168696908.1"
                     /sec_met_domain="ThiF (E-value: 1.4e-23, bitscore: 81.6,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLNDGQRLRLLEHVVINVMPADCSGEESMIFNTTRRTLRVHGQA
                     LHNVENIVLPLLDGSRTIGEIRNAIGEQLTDSSLNQCLEFLMDNRLVEESLENTNDLDS
                     RAYLLPQISLYHELGFDQKDARKNLANARIAVFGLGGSGLIAAINLATAGIGFVRLCDD
                     ACILPADSLMMSGSGLNKIGTFRSVEAARQIESIGGITEAEIVTDGLNDDATINSLLED
                     VDLVIVATDAVSVNLAYRLNRLCLKMQRPLLPGGAAGVEGTVGPLVFSQDKPCYLCYRM
                     RSMACSKFPEAELAVEQLLNRERRSQPRLRENLPIGQMLVGSYLALEAVKMMLGLAIAT
                     DGKLLHIDLLGTKLTHNVVLKKPGCPHCSTQKGNTSTSSVHRREEGTGNGKR"
     gene            complement(13483..13854)
                     /locus_tag="HGD76_RS20855"
                     /old_locus_tag="HGD76_20860"
     CDS             complement(13483..13854)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951270.1"
                     /locus_tag="HGD76_RS20855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20860"
                     /product="hypothetical protein"
                     /protein_id="WP_168696909.1"
                     /transl_table=11
                     /translation="MLAEKTLDQPIYVEMRLVDQADKLERLLLSEEEIAALEKLAMSGE
                     FDSEITQRWFANCGGCGGGGCGGCGGCRGCRGCRGCGGCGCRGCGGCRPCGGCGGCRFA
                     DHGTASNLMGDIDELGFGY"
     gene            14850..16661
                     /locus_tag="HGD76_RS20860"
                     /old_locus_tag="HGD76_20865"
     CDS             14850..16661
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080052.1"
                     /locus_tag="HGD76_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20865"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_168696910.1"
                     /transl_table=11
                     /translation="MSDLRVWFGDYTRILFRSLPLLWTAAPQEMVFLIAVTLLQGFLPG
                     VSVWITKLVVDTVATALTTGQALDDSILWYLVAAWVGALLLENLLYPWILALQGNLNDK
                     LAAHISLLLMRKADSFADLSRFEDAEFYDELQILQQQVSYKPLNLVENLVELSRSLITL
                     IVVIGLLIPLAFWIPLVIAIATIPQIVVSSQYGREIWITLFENSPQARKMQYYTSLMLT
                     DTYAKEVRLFQLGAFFMQLYGQAFQSLHQSMGHLRGKQAFWSSSLAILSTLGNGFSFYW
                     VVKQAFRGLLSPGSVLLFVQSLTYFQNSLERFVSHWLDLFENIIYMQQFFNFLDSPTPM
                     VLNIPGEKIPTPIRAGITFEQVDFHYPDGRLALKDISFTLYPGQTVAIVGENGAGKTTL
                     VKLLTRLYDPTTGRIIVDGIDLRDLNLEQWRQQIAGVFQDFGHYALTLGENIALGNLAA
                     LEHREILRYAVEKADIVKLVDDFPSGEDTQLGKQFGGTELSGGQWQKLALARAFVRKEA
                     QLLLLDEPTAALDPRSECDFYLRFVELAEGKTTILITHRLASVRMADRILVLKDGHLIE
                     DGTHQELLQLGGEYTALWNMQVEQYGV"
     gene            16759..18081
                     /locus_tag="HGD76_RS20865"
                     /old_locus_tag="HGD76_20870"
     CDS             16759..18081
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080051.1"
                     /locus_tag="HGD76_RS20865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20870"
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="WP_168696911.1"
                     /transl_table=11
                     /translation="MNIKNPIPWLIGLTAFGLSGMGIAYFSHWYQPSKNDITDLTVPVT
                     TSNLNIKIKTNGVVQPVRKINISPREAGRIAKLYVDEGSSVQKGQLIAEMERQAFQAQV
                     NQYRALLWKAKAELQEKRKGYRPEEIAIAQADVRKYTAQVREAQSRLALASQRVKRRQY
                     PTSQGAVSRDDLDAVLNEEKSARDNLQQAKFSLISAQQQVKKLLSGYQTEEITKAGAEV
                     AQATAQLQFYENQLENTFIRAPFAGLITRRFAQAGDFVTPNTSVSTNEGGTSASIAELS
                     SGLEIEAKVPEVNMAQIQKNQPVEIRLDAFPDQVFQGKVRLIAPRGVKEENVTFMRVKI
                     ALATGQDKLKVGLNVKLTFLVNDIRNALVIPSAAVVSGKGGQVGVLLPDKDNQAKFHPI
                     QVGITSGDKTQILGGLSEGERVFIQPPANQLIDGIDRVTGS"
     gene            18213..19418
                     /locus_tag="HGD76_RS20870"
                     /old_locus_tag="HGD76_20875"
     CDS             18213..19418
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080050.1"
                     /locus_tag="HGD76_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_20875"
                     /product="ABC transporter permease"
                     /protein_id="WP_015080050.1"
                     /transl_table=11
                     /translation="MNLLESVNMAFATLAANKLRSGLTMLGIIIGNASVISMIGIGQGA
                     QNLVLGKLEAFGANQLTVYSTFEDDEGMKFPDAQLVLSDAEAIKAQVPSVSKVAPIDEQ
                     KMSISFNNKLTSTTVKGTTFDLLAVQNLTLVTGRMFNQIQQQEQAQVTVLGAETSRKLF
                     GDENPVGQEVLINNISFQVIGLLQSKGAFGGENSDQTAIVPLTTFANRLVGRRSISDIP
                     LSYILVTAKNRDSIRAAGFQITNILARLHGKKDVAVFANKKFQELVVQVTGVLSLLLAL
                     IAAISLLVGGIGIMNIMLVSVTERTQEIGLRKAVGATQNDILAQFLIEAIILSLSGCLL
                     GIIVGVGGTMPLAFFTPLTPQFPLYAIVVAVGISGSIGLIFGVFPARQAAMLDPIVALR
                     SG"
     misc_feature    21323..21337
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.87"
                     /tool="antismash"
ORIGIN
        1 gaacctattg acgcggcttt gtgctgtggt agtgcggggg tttataatat gttacaaccg
       61 gaagttgcgg aagaattagg taaacaaaaa gcccagaatt tattaaatac tggtgcagat
      121 ttaattgctt ctcctaatcc tggttgtagt ttgcagatta gtaaatattt gcaggggaaa
      181 actatttctg tcatgcaccc aatggagtta ttagattatt caattcgggg tgataagttg
      241 gagatttaga acattataat atccagatcc ccgacttctg agattaattt ataatgtatt
      301 tacaagatcc agaaagaagt cggggatctt atttcagttc attattattc aagactaaca
      361 actacagctt gacctttcac taatgtagat aaaatatcta aaatctttgg aactaaaggt
      421 tttaacccac attccgcacc ccaaactgtc acagatcaaa aaagccctta aagtagtaca
      481 taataactaa agtcaattca aaaattaaga tgcttaatga taataaatag cattaaaagc
      541 gaaatggtgt gagaaagtga aacttggtaa caaaagaaaa aaatgaattg ataaccaaat
      601 aagaaataat tgataacaac agaagtatga gaggaaactg ttgtgaaatt aaaacctcaa
      661 gaaatggaaa ttcagaacat agaccatcta gggatagtag caggaatcat agattcaatc
      721 ggaatagtag aaataataaa tgaattaata ggagtcgaaa aagacgaaaa agtaaatgca
      781 ggtcaagtgg taaaagccat gataataaac gggttaggat ttgtctccaa accgttatat
      841 atgtttccca aatattttga aacaatagcc tgtgagcatt taataggagc aggagtaaaa
      901 ccagaatatc tcaacgacga taaattgggg agagtcatgg ataaactgtt tataaaaggc
      961 ttagatacaa tcttttttat catagcctta aaagctgctc aaaaatttgg agtatcacta
     1021 tcaacatcac atctagactc atcatcaatg cacgtgcatg ggcagtataa cactagctta
     1081 ccattcgtaa tatttgagag tcaaaaagta ggaaataacc aagaattaga agaattagca
     1141 gtaaaatcac caaaagaaat aaccatcacc tacggttatt ctcgtgacca tcgtccagac
     1201 ttaaaacagt ttatcataga aatgatatgt tcaggagatg gagacatacc aatattttta
     1261 aaactagcat cgggaaacca agctgactca tcatgttttg gtaaaatagc agtagaatat
     1321 caaaaacaat tagaagttaa tagtctcatg gttgctgacg ctgctttata tacagaatca
     1381 aacctgaaaa tgatgtcaga attacgttgg ttatgtcgag taccattaag cataaaagca
     1441 gcaaaatcat taatatcaac attagcagaa tcagaattta ttgatagtac aataccagga
     1501 tataaattag catcaaaaat ccaaaattat gcagggatag aacaaagatg gttagtagtg
     1561 caaagtcaag aaagaaaaga atcagacctg cataagctca cacaaaaaat taccaaagca
     1621 gaatcaaaag ctgtgcaaga tttgaaaaag ttatcacaag agagacttgc atgtgttgca
     1681 gatgctatca aggcattatc aaaattatca aaacaattca aatatcatca aattcacgaa
     1741 agtacggtga ctcaagtaaa atctaataaa aaagatactt caggagaaat atcctatcaa
     1801 atatcagcta cagtctccca ggatgaaagt aaaattaata cagaattgct gagtgcggga
     1861 cgttttatta ttgcgacaaa tgttttagat tcaaaggaac taagcaatga ttctatgctc
     1921 agggaatata aagctcaaca atcatgtgaa agagggtttg gttttctcaa agacccatta
     1981 ttttttgccg acagtatttt cctcaaaagt cctgagagaa tagagtcttt gggaatgatt
     2041 atgggtttat gtctactggt ttatactttg gctcaacgtc atattagaaa tgctcttttg
     2101 gagtctaaat caacaattaa aaatcaatta ggcaaagcaa ctaatcgtcc tactttacgc
     2161 tggatttttc aatgctttca gtgtattcat ttggttacac tcaatcagga gaaacatatt
     2221 tctaattgga ataaggacag agattttatc ttgagtcttt taccagatga ttgtttacgt
     2281 tactatcaat tagtcagcta attatcatct ctatcaattt aatttctatg agtaaagatg
     2341 agctaatctc tttgatttat agctccattt tactatgtct ataatttcgt tttttacttt
     2401 aattgattag tctaacttat aattatttct tgaatatctc ctttcgctta ttcattgacc
     2461 tctccaggtg gtgtgcattc ttattgcttc ttattgagaa tagattttga tgacattttt
     2521 ggtgatttac tgtttctcaa atgccttttt tcactttatc tgtttgtttt gatgctccct
     2581 ttgattttca tctccgtaat ttccctctgt aacattttgg ggtgcggaat gtgggttgta
     2641 gggaaggggg caggggggtt aggtttttag agattatggg tttcatctaa tactttatgg
     2701 ctaacgccac gcttcgctat caaacaacct ctaaacatca accccttttt ctgtttctat
     2761 attctttctt gttcctgact cctaaccttc acgataaata gcatctgcta ctaaagtaac
     2821 ttcttgcatt tcctgaacat cgtgaactcg gaggatatct gcaccattga aaatggctgc
     2881 acaacaggct gcggctgttc cccaaactcg caatttcgga tctggttgat gaagaatatt
     2941 acctataaaa cttttacgag atgctcccac caaaattgga gaattgagtg tttttaatga
     3001 ttctaaacgg cgaaaaattt ctaaattctg ctcatggttt ttagcgaaac caataccagg
     3061 atcaataatt attttatttt tgtcaattcc caaaagtgta gcctttgtaa tttgctcaga
     3121 taaaaagtta taaatatcag caattagatc ttcataatca gtacattttt gcatagtttg
     3181 gggagttcct tggatgtgca ttaagacaat cggcacattt aaactagcaa cagttggcaa
     3241 catttgcggg tcaaatgttc cagcagaaat atcattaatt atatctgctc cagcgataat
     3301 tgctgcttta gctacttcgc ttctagtagt atctatagaa ataggaatat caataactgg
     3361 acgtagcaac tccaagaccg atagcacccg ctcaagttct gcttctaggg atatttgttc
     3421 tgcccctgga cgggttgatt gtccaccaat atcaataata tcagcaccag ccgaaaccat
     3481 tgcttgtgcc tgggttaaag ccgctgaggt ggtgttaaat ttaccaccat cactaaaact
     3541 atccggggtg acattcaaaa ttcccatgag ataagtccgc tttccccagg taaaacagtg
     3601 atctcggata gttaagttgt tagacatgaa attttttaac ttttaattga tgtatcccca
     3661 atccccaatc cccattattg atagttgaca attctggcaa aactatcagc ttttagacta
     3721 gcgccaccaa cgagagcgcc gtcaatttct ggttgtgcca taatttcatc aatattattc
     3781 gggttgactg aaccgccata ttggatagga actaggggat tagttaattg actgcgaatt
     3841 aagccaatta cccgatttgc ttccttggat tcacaggtat caccagtacc aattgcccaa
     3901 attggttcat aagcaataat taggtgagtt tgatcaatat ctaccaagtc tttttttaat
     3961 tggttgctaa ttattgattc tgtttcccct gcgtctcgtt gttgtttagt ttccccaacg
     4021 caaagaatgg gtgtgagtcc atgcttttgg gcggctttaa gacggagatt gacagttacg
     4081 tcagtttctc cgaagaattg ccgtctttca ctgtgtccaa caatgacaaa acgcacaccg
     4141 atttccgtga gcatgggggc agcaatttcg cctgtatatg ctccatgttc ttcccagtgg
     4201 acgttttgtg cgcccaaatt tacacggcta ccatgtaaag atttggacag caggcttaag
     4261 tcggtgaagg gaggacataa taccacttct cgttctgagg gggtttcctc caggtgaggc
     4321 aaaaatccgc ttaaaaattc tgcggattct gcctgggttt taaacatttt ccagtttcca
     4381 gcaataacga ttttccgcac aggtgattta atcaagattt taatgctact agatgcactt
     4441 ttcaatgtac tactttttta ggactattca ctaagcagaa ttcactgact cacagaaata
     4501 gtcgtcagta gtaatatagg ctgatggttg gttttttaac ttagatagag tttttgtatc
     4561 aatcaaaaat taagctattt gccaaaatta ggtgagcagt tgcgcttgat tacgtaaacg
     4621 caactactaa attaaactaa ctgcaaacca gcttttgctt ctttggcaat tgcctctact
     4681 tcgtcattta ccaaggttgc tagaatagat ttagcttcat cactacccaa acgagttaaa
     4741 gcttgtacta ctctgtaacg tacttgccaa tctggattag tgctgtatgg aactaggaga
     4801 ggaactgctg ttggatctcc caattcacca aaggaactaa tggccgcagt ttgaattaag
     4861 ccattatcgg aagcaagtgc ttgggtaagc aattcaaagg ctcgcggatc tcctaattct
     4921 cctaatgtcg caataatgct aaactgtacc agccattctg gtgtttcttg gtaaagctgt
     4981 tgtaagtctt caaaagcgtc atgtaatttc agcgcaccta aacagtctgc tgcggctgct
     5041 tgaacatctg cttctggatc atgaagtaaa cctcggagga tattcaagga taatggtaaa
     5101 tcctgtgttc ccagggtatc catttgactt acggccgaat aacggacacg cgcactttta
     5161 tcctgaatgg caatttggac taattctaaa ccgatttgtg gttctagttc ccgaatttga
     5221 ttgactgcac gcaagcgatc gcctaaattg tcagaacgca gtaattctct tatactatca
     5281 gcagacacac tcatttaatt gtcctttgtc agttgttaat tgttagtagg ggcgaagcat
     5341 ttggaagata aattatcagt cattgccaaa aatagttctc caaatgcttc gcccgtacag
     5401 ttgttagttg tcagataata tttctctcct ttgctactct gcggccatag tgcgaataat
     5461 atcaccacga gttagaatac caattacctt acccgcattg tcaaggactg ggagacgatg
     5521 aactttgtga tcttgtatta attttgctgc ttctcttaat ggtttatcag gagaaatagt
     5581 taggggattt ttactcataa cttcccctac tgtttgtcct aaagctttat gtaaatctcg
     5641 ttcataggtt gcaggatttt gtaaataaat cacactatcc aaaatcatga tatatgcagg
     5701 gggagttata ccagtttctt gccacatcaa atctgtttct gagataattc cgactaattt
     5761 acctgcgtca tcaattacag gtaaaccgct aatgtgtttt tctgccagaa tttggatagc
     5821 ttcttttaag ggagtttgag gatggacaag gataggatca cgactcatta cgtcagcaac
     5881 ggttttagac atttttttac ttacaccttt tataaaagtc tttttaacta ttgtaggaaa
     5941 ttattggtaa caaataaaat acagttaata gattgttact tttcatttta aaaataaacg
     6001 ttgtttttgt tgaaaaagca tttttagcaa tgccattgta ccataaaagc ctaatttttc
     6061 aattaatttt aaccattcga ctaaggaagc agattttttt gatattttat gctgtccaac
     6121 tttttttacc aattgtcaac aggctcaaat aattatgcaa acgtcaactc taatatagga
     6181 tctctaaact atccaaactg cggcggtagt tgtggctttg taattatgcg tactactccc
     6241 actaataata ttaatggtac aagtagcaat caaattgaag gaattattgg tgtaattcat
     6301 tcctttcgct cacctgatca aaaaaatgct gaaactaatc gtcaattaat agatattcaa
     6361 aaacaaagaa gtgaaagtga gattgcgagt aattacatca aagcaattgc cgatgcttgt
     6421 caagccaaag attcaattag atcagaacta agtgctaaag gattagctcg aatttggaac
     6481 gtagattata aaaccttact tcaccctagc tgcggctcat aatattgaga attaatattt
     6541 cactttcttt ttcttttatc atttttgaat aataattttg tgattcatta tatcaacaat
     6601 gtgattttaa ctaatattgt ttcaactaaa taaacccgat attcccatat caaattaagc
     6661 cctaaataaa agcaaaaatc atggctaatg aaaaatcacc agacattcaa aatactaaca
     6721 atgccgataa caaagaacat aaaattgaat gttcttcctg tcaatgtaaa gggagaatcc
     6781 aaccttatgg ttcacgcaga ttatccaaat gtcaaaaatg ccaaggaaca ggtttttgtt
     6841 aaaatcaaaa ttgataatta aattcagatt tcgtaggggt ttagcattgc taaacccttc
     6901 ctaataacta tctaatttat caattctcct gatgatattt cctcaaagcc gcaataattt
     6961 ccctattcgc ttctggaaaa gtaaaattat ccaattcatc caaacttacc cagcgaattt
     7021 catcacattc tagggcttga ggaacacccg ctaaatgttg agaatgatga actatcaaag
     7081 tcacccgcaa atgagcataa gtgtggtcaa tagtaatcag atgttctcca actacaattt
     7141 ctatacccaa ctcttccgaa atttctcgtt taatacattc ttgaatagtt tcaccaattt
     7201 caatttttcc ccctggaaat tcccataaac cacccattgt cccagtagga agtcgtctat
     7261 caattaaaat ctgtttttgg tcattccaaa taacagcaac accaataatt ttatgaggaa
     7321 tagaagaact actttcattc atataattac tcagtaccaa taaataaaaa aactcagtag
     7381 actaattata tatctaccga gtttatattt attttgagaa ttttgctaaa tgttagtttg
     7441 atgtgttaat aaattaaata aaaaataccc cttggattat aatcaaaatt attgtaaaaa
     7501 aaatagttga gaatatcaag tcctgaaatg aacgttttta ggggcaatta ttgagaatat
     7561 tctcaagatt attgacaata gagaagaatg cgcttttttt tgaaaatctg attgaaaaaa
     7621 cttataggtt tcgggaaaaa ccataagtta aaaagtagtt gggttgactg gagaaaaccc
     7681 aacttgctaa ttatcaagtt acggtaatca tcatacctgc actttcattc cctaatctat
     7741 ccacagcaca gactgcataa gttcctggta gaactgtcgc aaaggtagta ccagcggata
     7801 aaatgcgttg aattagccaa ttattgctat tttgcaaata cagagtccaa gaacgcacaa
     7861 gttgatcatc tcctgcttcc cagtttaatt taccatccat aaacttgagg tttttgggag
     7921 gtggtggcgt aatggaactt tgccatgaca tagtgggaat taaggcaggt gtgggataat
     7981 aggttttaaa ttggtcagcg atgccttggc gattttcctg aatgccagtc atactaaaaa
     8041 agatattacc taaagacaaa ttacctgcta aattccgaga aatctgaatt tgcttggcaa
     8101 tttcgctatt tttccattcc ttaccatcta attgtccgag attattacct gcatagatgt
     8161 gacgctgttt agtattgatt tcggtccacc attgcagtaa agtttgatag gcttgtttgg
     8221 tttggtcagt acgccagtag agttgaggag caatataatc tatccagcct tgctgtaacc
     8281 attttttaga atctgcatag aggacactgt aggcatctaa accgacaatt cccgcaggtt
     8341 ctccagaacg ataaatacca aaaggactaa ttccaaactt tacatgaggt ttggtggctt
     8401 taattccttg agaaagtcgc aataccattt gattgacgtt ttcgcgtcgc caatcttcta
     8461 tatttagttt acccccactg gtttgatatg ctgcgtaggt tttatgatca ggaaaaggtt
     8521 gaccagatat gggatagggg taaaaataat catctaaatg aatagcatct aaatcataac
     8581 gacggacaac atcaataatg acgttgtatg ctctgtcctg aacgattttt gcaccggggt
     8641 ccatccattg ttgagtaccc cattgataaa caacttcggg gtttgtaact gcaatgtggg
     8701 gacgaacggt agaaactcga ctatttgctc tagcacgata aggattaaac caagcgtgta
     8761 attcaatatt gcgttgatga cattcggcga tcgcaaactc caaaggatca taaaatggtg
     8821 atggggcttt tccctgagtt cccgttatcc aagcactcca aggttctaat tccgaagcat
     8881 ataaagcatc accttctggt cttacctgca aaactaaagc attgaaattc aaagcttgta
     8941 actgtttaat aatcgctaat aattccgctt tttgctgttc cacagccagt cccggtcgag
     9001 aaggccaatc actattccat acagacgcaa cccaagcccc ccgaaattcc cgccgatgat
     9061 ttagcttaac agtatgagca gttgtaggtt tagggatggt tggaggaaca acaatataat
     9121 cagtcgggat tttttccgct tttcctaaat atactaaagc ctgataaacc attactgcga
     9181 cttctgcacg agtagcggct gttttgtaat taagtatttt tttgttcgga taactaacta
     9241 cccaaccagc acgagttgct aacgcgactt gattaatccc atagtaagga attaaagatc
     9301 catcttgata aatttctgcc agtttactta ctaaatctag gtcaacttga ccagcaagtt
     9361 ctaagccatt gaccattgct actaaagcat cacctctagc aataccttca tctatcccaa
     9421 agcgccgatt tggataaccc gttaaaaatc cagtttcata gaccttttta atcgcgttta
     9481 tcgcccaatg cgtctgggga acatctacaa aaggtacata ttcccgtttg acaggcaggg
     9541 aaaaaattgc ggcgataata gctgcaaatt cagcacgagt cacagggtta ttagggcgat
     9601 aagttccatc agaataccca ctgagaattc gccgtgctgc taaagcttca ataaaccctt
     9661 gggaccaatg accttgaata tccgagaatc ttatagaact agataccata ttgaatgaga
     9721 tagttgcatc ctttgttaat ttagcaatat ttaagtcata gctgctaagg gacttccggg
     9781 aaataaatta tcccaataaa taaataaata aatgaataaa cgaaccacag aggcacagag
     9841 aacacagaga gagaagaaat agagagggtt tttgcgttag ttttgggaca tttttttatt
     9901 tggaagtccc taatgacagt aaagaaaaat tcctcaaact cctcctctct gagcctctga
     9961 gactctgcgt gttagcgaag cggggcgaag cctaaaaaaa ttatcccttc ctcccaacag
    10021 caacacaata aagagcagca tgacgttctc catcaatacc caacatagcg tttaaactca
    10081 tatcatcaaa tccaccaata cagagactat ccagtcccaa ttgcccagcc attaagcaga
    10141 tattttcggc ttgatgacca gcttctaaaa gagcaaagcg gtagccccgc gccccatact
    10201 tgcccattga gcgcataaaa accgatgtga acacaaacag agcattggca ttgagaatgt
    10261 aatcctgctg caataatggt tcgacaaaat ccgttgggac ggcatcattt acccaatgta
    10321 acccgtgtcc gcgaggttca tagtgatata gtccgctaac caaacctgct accgcttgtg
    10381 tggatacgaa cacctccaga ggataaagtc cccccgcaga aggagagtta cgagcctcat
    10441 agcattgacc atccacttcc cgcagtccgt tgagtccaca tcctgagtcg agcagttgag
    10501 ctaaggcgac cagtggcatt acctgacgct caaatgatcg gacagaagta cgtcgcgcta
    10561 aaagctcaga cagcgggtta tcacgtggtg cttttggcaa ttccagaaaa ggagcttctg
    10621 gcagatatcg cagttcagta gccggaaccc gcttaacata acttttaggg actggagagt
    10681 ttaaatgata gagaagagct aactccagag gttcattggc tgtgtgcatt gtcctaattt
    10741 cctcgtttgt ttaatcaaca acccgctaac ggctcgctga cggctgtttt aagccatagg
    10801 atgtgggcag ggatttaagt catttttagt ccgtcggcca ccataaccca acctttctgg
    10861 gacctcgaat aaccgccgag tccccagtgg ttcttgtcca taaccaaaat agatagggac
    10921 tatttcactg gctaaagtcc gcaccactcg aaaaccaaga gaggcgatat caggggcagt
    10981 aatttccact acataaggtt caacacctac tgaatttaac ttggcaacta ctgattgcaa
    11041 attctctgct tcagactcag attcgtaagt tggtaaatat tcaacttggc aagattggtc
    11101 atggtgaaat agaaaatcca actcacctag ccgagccgtc gtgtaaaaaa aagcgttgtg
    11161 atcatctaaa ttctgaatat cactatattg atggagatta gccccagcgc cagttttcat
    11221 ccgttctatg tcaccaaagc gagcttgaca aacttcaaaa actgccttgt ggaaagccgt
    11281 tggtgcatca agatgacatc ccataccagt ggacacagcg ggacctttgc ccgaacggtc
    11341 aattaacatg gccatgaaaa ccgggacttg aatatcagta gttaactcga agacaactaa
    11401 ctcaatgcca aagcgggcat agtgacgggc aatttccgtc tcaattcctg gcaggtggtc
    11461 gaaatagatg cgtggggcag ggaggcgatt taaccaggta ataatgaaag catcacgctc
    11521 aattaattcg cacaaacccc ggtaggcagc aaactcccaa gatggtccac tagccatacc
    11581 gttagaacta acaggtaaaa tataatctcc tggttggtcg ccaccccagt ctaaataaac
    11641 caaaaaggct ggaactaaaa cctgttgctt actggttaag gaaagggcat tcatccacga
    11701 agtctgcgtt gtggaattaa aggccgggaa agggaaagtt gggtgagagt attgttggtt
    11761 agaaaaagga gaaaaaacgg tcgaagggac ggcacgatta cctaattctt cgtagctact
    11821 aacaattatt tgccgataat caacaatacc accgcagtat ctttctatag cttcgcccac
    11881 agccccaaaa atggcttgtt catccgtcat gcccctaccg acaccgtagc gtaaatcatc
    11941 aggctgttta cgaagctgaa aattggctaa ttcggcttgc cacaaaacgg gaaaatcgaa
    12001 ctctgtataa ggcttggtga atctatctaa ggagcggata acgccagtat gggggctgac
    12061 tagatcttgc caacggggac gtgcaatcgc tgtattcact gtgttctctc ctatcttttt
    12121 ccgttccctg ttccctcttc cctgcgatgc actgagcttg tcgaagtgtt ccctttctgt
    12181 gttgaacaat ggggacaacc tggttttttg aggacaacgt tatgagtaag ttttgtgcca
    12241 agtaagtcta tatgtagtag ttttccgtca gtagcgatcg ccaaaccgag catcattttc
    12301 acagcctcaa gagctaaata gctacccacc aacatctgac ctatcggtaa attttcccgt
    12361 agccgtggtt gagaacgccg ttctcggttg agcaactgtt caaccgccaa ttctgcctct
    12421 ggaaacttgg aacaagccat agaccgcatc cgatagcata gataacaggg tttgtcctgg
    12481 gaaaaaacca acggaccgac agttccctcc accccagccg ctccacccgg tagcaatggg
    12541 cgctgcatct tcaagcatag tctgttcaga cgataggcta gattcacaga tacggcatca
    12601 gtagcgacaa ttaccaggtc aacgtcctcc agcaaagaat tgatggttgc gtcgtcattt
    12661 aaaccatctg tgacaatctc agcctcagtg attccgccaa tggactcaat ctgtcgcgct
    12721 gcttccacac tccgaaaagt gccaatttta ttcaatcccg aacctgacat catcagggaa
    12781 tccgccggca ggatacaggc atcatcacac aaacggacaa aaccaatccc agcagtagct
    12841 aggttaattg ctgctatcag tcccgaacct cccagtccaa aaacagcaat tctcgcattg
    12901 gctaagtttt ttcgagcatc tttttggtca aagcctaatt catgataaag gctgatttga
    12961 ggaagtaaat aagcacgact atctaaatca tttgtatttt ctagagactc ttctactaag
    13021 cgattgtcca tcaaaaactc taaacattga tttagggaag aatctgtcaa ttgttcgcca
    13081 attgcattgc ggatttctcc tatggtgcga gagccatcta gtaaaggtaa aacaatattt
    13141 tccacattat gcagagcttg accatggact ctcagggttc gtcgtgttgt attaaatatc
    13201 atactttctt cgccactgca atctgccggc atcacgttaa taacaacgtg ttctagtagt
    13261 cggagccgct gaccatcatt taatttcata gactttatta ttgccaaggt ttaatcctgc
    13321 tataaatagc gatcaaaaaa attaaaaacc ctgtttctga caaccgaaaa tattgaataa
    13381 aaacagggtt tctgttgatt tttttggacg aatttgatga ttttaacctc aagaaactcg
    13441 gttgatcaaa ttactaaatc cctattctgt cttcaggttt agttagtatc caaatccaag
    13501 ctcatcaata tctcccatca aatttgatgc tgtcccatgg tcggcaaatc tacaaccacc
    13561 acagccaccg caaggtctac aaccaccaca gcctcgacag ccgcagccac cacagcctcg
    13621 acagcctcga cagcctcgac agccaccgca accgccgcag ccaccaccac cacagccacc
    13681 gcaattagca aaccatcttt gagtgatttc agagtcgaat tctcccgaca ttgctaattt
    13741 ttctaaagct gctatttcct cctcagacaa cagaagtctt tctagtttgt ctgcttgatc
    13801 taccaaacgc atttctacat atattggttg gtcgagtgtt ttttctgcaa gcattgtcac
    13861 ctactccttt caacttaaat ttaataacct ggttgcaaat ctctatttga gttgataaaa
    13921 agctggaatg atgtttttaa atcaaccagt tttcacaaat tcttcaaaat tacactagtc
    13981 cacaacggtg taagtcaact aaccattaaa gattcatgaa aagcttatac aatctgcttt
    14041 tttaattttt aattgttaat ttttaattcc gccctgcggt attagtccac aaaagagcaa
    14101 aaccactttc tgattagtgt ggggaactat cagatgggat ttatattctg tgaaaccttg
    14161 tcttttatga aaattgctct tttcagggtc gccaattatg tctttgtttg cttatccgat
    14221 tcttacaaga atatcttttt ctagataatt ttcttctttc ttaagtagta ttattttatc
    14281 tcttgataga ggaggaatca atttatgaaa ttgtgctaat ggtgaatgaa aattcttgta
    14341 acagagaata attttatagt taaagcgaat ttaaaataat gcttatgacc gattgaaaag
    14401 cgtagctcta tttctctata attactcctt tagtaaggtt atcagacttg attacttttc
    14461 aggatttatc taaaaatagc aaatcaaata tctgatcatc ctttaatgat caaggttata
    14521 ttggttctag gctgttttga tatccgtgaa aattctgaac aacagacatt tgaaaatttt
    14581 tgaaatattc atccctaaga agtggagaac aaaatttttc aaaagtagaa tattaaagta
    14641 atagatagca gaatccgaaa aaattcctga tcaactttgc agcgactgat tgctggttaa
    14701 gttggaaaag gataaaattt gatcactgca tattttattc ttgaaaaata aactctccca
    14761 agtgataagt ctagttatta ctcagaaaat atcaaacttt tggaaatcag agaattatct
    14821 aaccctttat aaaaattcag gtttaattaa tgagcgattt aagagtttgg tttggagact
    14881 acactagaat tttatttcgt tctttaccct tattatggac agcagcacct caagaaatgg
    14941 tgtttttaat tgctgtgact ttattgcaag ggtttcttcc tggggttagt gtttggatta
    15001 ccaaactggt agtagatact gtggctacag ccttaacaac tggtcaagca ttagatgatt
    15061 caattttgtg gtatttggta gcagcatggg tgggagcttt gctattagaa aaccttctct
    15121 atccttggat attggcactt caaggaaatc tcaatgataa attagcggct catattagtt
    15181 tattattaat gcgaaaagct gatagttttg ccgacttgag ccgttttgaa gatgctgaat
    15241 tttatgatga gttacaaatt ctgcaacaac aagttagcta taaaccctta aacttagttg
    15301 agaacttggt agaattaagt cgctcattga ttactttaat cgtagtaatt gggctattaa
    15361 ttcccctggc tttttggata ccattggtaa ttgcgatcgc tactataccc caaatagtag
    15421 tttcttcgca atatggtaga gagatttgga tcactttatt tgaaaacagt cctcaagccc
    15481 gaaaaatgca gtattacacc tctttaatgc tgactgacac ctatgccaaa gaagttagat
    15541 tatttcagct aggtgcattt tttatgcagc tttacggaca agcatttcaa tctttacatc
    15601 aatccatggg tcatttgcga ggtaaacaag cattttggtc atctagttta gctattttga
    15661 gtacattagg aaacgggttt tctttctatt gggtagtcaa gcaagctttt agaggactgt
    15721 taagcccagg aagtgtactt ctgttcgtac aatcattaac ttacttccag aatagtttag
    15781 aaagatttgt cagtcattgg ttggatttgt tcgagaatat tatttatatg cagcagtttt
    15841 tcaactttct cgacagtcct acccccatgg tattgaacat acccggagaa aaaatcccca
    15901 ctccgattcg tgcaggtatt acttttgagc aagttgattt tcactatcca gatggtagat
    15961 tagcactcaa ggacatttct tttacccttt acccaggaca aacagtcgcc atagttggcg
    16021 aaaatggcgc aggtaaaacc actctagtca aactattaac caggctttac gaccccacca
    16081 caggacggat tatcgttgat gggatagacc ttagagattt aaatttagaa cagtggcggc
    16141 agcaaattgc gggggttttt caagactttg gccattacgc actcaccctg ggagaaaata
    16201 ttgcattagg aaatttagca gcgttagaac atcgagagat tcttagatat gcagtagaaa
    16261 aagccgatat cgtcaaacta gttgatgatt ttcccagtgg ggaagataca caattgggta
    16321 aacagtttgg cggtacagaa ctttctggag gacagtggca aaaattagct ctagctcgtg
    16381 cttttgttcg caaagaagcc caactactac ttttagatga acctaccgca gcccttgacc
    16441 cccgcagcga gtgtgatttt tacctgcgtt ttgtggaact agcggaaggc aaaaccacca
    16501 ttctgattac ccacagatta gcctcggtgc gaatggctga ccggatttta gtcctcaaag
    16561 atggtcattt aattgaggat ggtactcatc aagaactttt acaactggga ggagaatata
    16621 ccgcgctttg gaatatgcag gtagaacagt acggagtgta atacaaaaaa acttaattct
    16681 aggacttacg cagaaatcat cttccactct catttctctg tgtcctctgc gcctctgtgg
    16741 ttcgttattc catcagtcat gaacatcaaa aatccgattc cttggctcat tgggctgact
    16801 gcatttggtt tatctggaat gggtattgct tacttcagcc attggtatca acccagcaaa
    16861 aatgatatta cagatttaac agtccctgtc actaccagca acttaaacat taaaatcaaa
    16921 accaatggag ttgtccaacc agttcgcaaa attaatatta gcccaagaga agccggtcga
    16981 attgccaaac tctatgtaga tgaaggtagt tctgtccaaa aaggacaatt aatcgcagaa
    17041 atggagcgtc aagcctttca agcccaggtg aatcagtatc gagcactgtt atggaaggca
    17101 aaagccgaat tacaagaaaa gcgtaagggt tacagaccgg aagaaattgc catagctcaa
    17161 gctgatgtgc gtaagtacac ggcgcaagtg agggaagctc aatctcgctt ggcattagcc
    17221 tctcaacgag tcaagcgcag gcaatatcct acatctcaag gagcagtatc ccgtgatgat
    17281 ttagatgccg tcttgaacga ggaaaaaagc gctagggata acttacaaca agctaaattt
    17341 agtctgatat ctgcccaaca acaagtgaaa aaactacttt ccggttatca aactgaggaa
    17401 attaccaaag ctggtgctga agtagctcaa gcaactgccc aattacaatt ttacgaaaat
    17461 cagttagaaa atactttcat tcgcgcccct tttgctggct taattacccg acgttttgcc
    17521 caagcggggg acttcgtaac tccaaatact tctgtttcta ccaatgaagg gggaactagt
    17581 gcctccattg ctgaattatc cagtggtttg gaaatagaag ctaaagttcc cgaagtgaat
    17641 atggcgcaaa ttcagaaaaa tcagcctgta gaaattcgcc ttgatgcttt tcctgatcaa
    17701 gtttttcaag ggaaagtccg tttgattgct cctagaggag ttaaggaaga aaatgtcact
    17761 tttatgcgag tcaagattgc tttggcaacg ggtcaagata aactcaaggt agggctaaat
    17821 gtgaagttaa cttttttggt gaatgacatt cgcaatgcct tggttattcc ttcggctgcg
    17881 gtggtttctg ggaagggagg gcaggtgggt gtactgttac cagataaaga taatcaagca
    17941 aaatttcatc cgatacaggt aggaattact tctggtgata aaacccaaat tttaggcgga
    18001 ctttccgaag gtgaacgggt ttttattcaa ccaccagcta atcaattaat tgatggtatt
    18061 gatcgtgtta ctggatctta gcctactctc tttttactgg aagaatattg attaaatgaa
    18121 ccacgaagga gcgaagaaca cgaagaaaga aggaagaaga aagaaggaag gaagaaatta
    18181 agtaatttta tgctgggaag ggattatgaa ttatgaattt gttagaaagt gtgaacatgg
    18241 cttttgctac tctcgctgct aataaacttc gtagcggact aactatgttg ggaataatta
    18301 ttggcaatgc ttcagttatt agtatgattg ggattgggca gggcgcacag aatcttgtgt
    18361 tgggtaaact tgaggcgttt ggagcaaatc aactgacggt atatagcact tttgaagatg
    18421 atgaaggcat gaaatttcct gatgctcaat tggtgttatc agatgcagaa gcaattaaag
    18481 ctcaagttcc tagtgtgagc aaagttgcgc ctatagatga gcaaaaaatg tcaattagtt
    18541 ttaataacaa gttaacatca acaactgtta aaggcactac ctttgatttg cttgctgtgc
    18601 agaatcttac tcttgtcact ggcagaatgt ttaaccaaat acaacagcaa gaacaggctc
    18661 aagttactgt tttgggtgca gaaactagtc ggaaactttt cggcgatgaa aatcctgtag
    18721 ggcaagaagt tctcattaat aatatatcgt ttcaagtgat tggtttattg caatcaaagg
    18781 gtgcatttgg tggggagaat tctgatcaaa ctgctattgt tcccctcaca acatttgcta
    18841 ataggttagt gggacgcaga tcaatatctg atattcctct cagttatatt ttagtgactg
    18901 ccaaaaatag agatagtatt cgcgccgcag gttttcaaat tactaatatt ctggctcgac
    18961 tacatgggaa aaaagatgtg gctgtttttg ctaataagaa gtttcaagaa ttggtagttc
    19021 aggtaactgg tgttttgtca ttattattag ctttaattgc ggctatttct ttgttagtgg
    19081 gtggcattgg tattatgaat attatgttag tttctgtgac tgaacgtact caagaaattg
    19141 ggttgcgtaa ggcagttggg gcaactcaga atgatatttt agcgcaattt ttaattgagg
    19201 caattattct ctctctctct ggctgtctgc tgggcataat tgtaggagtt gggggaacta
    19261 tgccgttagc cttcttcacg cctttgactc ctcaatttcc tctatatgcg attgttgtcg
    19321 ccgtgggaat atccgggagt ataggcttga tttttggtgt atttccagcc cgacaagcag
    19381 caatgttaga cccaattgtt gccctaagaa gtgggtgatt attgagattt tttaggtcac
    19441 tccttccaat tcctcatcag tcaaatcata taactttcgc aacttatcca acttatctcc
    19501 atcagcttgc cataaaccgc gtcccgatgc ttccaacatt ctccccacaa tatttctaaa
    19561 agcttcagga ttggccttgc gtaatttttc cgccatttct ggatctaaag cataagtttc
    19621 tgcggcttga tcataaaccc aatcatcttg aaaatcagca gtaccacccc aaccaattaa
    19681 agccgtcatg cgttgggaaa tttcaaaagc accaccagaa ccttgattag ccattgcgtc
    19741 agcccatttg ggatttaata acttagttcg gtattccatt cgcaataaat catctaaatt
    19801 gcggggtgtg gtgtctttag aaaaactttc gacaaaactg gcctgtactt tttttccact
    19861 ttgcttttcg gcagcttttt tcaaacctcc cgtattggca taatattcct gaatatcagt
    19921 taaaccatat tctaccgaat ctatttcttg aacaatgcga tcgctagttt ttaataatgt
    19981 ctgcaatatt tctggtctag cttgtccttt atcatttcta ccataactaa aaacattgcg
    20041 actttcccaa gtatccccca actcttctcc agattcccaa ttaccatcaa caactctatc
    20101 attaaccaaa gaaccaaaat caccagaggg attagaaaac aatcttgcgg aagaattttc
    20161 cacaccttgg gcttttaaaa tcaaagcgtg ttttctaata aaattcattt cctccagttc
    20221 atcaatttcc gcagcccgtt gaaataaatc atctaacaat tcaatgatat tcacaaaact
    20281 atcccggaaa atacccgata gatttgctaa cacatcaatg cgcggatgtc caacatcagc
    20341 aagaggtttt aaatcatatc taacaatgcg tccagttccc tcctttacag gttcagcacc
    20401 caccaattcc aagagaattc ccaaagattc acccttagtc ttaatagcat ccaaacccca
    20461 taataaaact gctacagttt cgggatattt cttattttca tctaaagact gagaaataat
    20521 tttcttagca atttccttac cccgtgcaaa agcagcagga gaaggcattc tataaggatc
    20581 taaagcatga atattcctac ccgtaggtaa caccccagca ccatccctta acaaatcccc
    20641 accaggtgca ggaggaataa actcaccatt taaacccctt aaaagattag ttaactcatc
    20701 agtagattga tttaataacc ttgtaatttc ctcctcctct ctgtgttctc tgtgcctctg
    20761 tggttcgttt ccaaaataag catccaaata accagttaac tcctcctgat ttggtgcttc
    20821 acctaaagta tgtaaaccag aagaaaaaag acgattttcg agaacttgga gatactcata
    20881 caacttcacc aaataatgat caaaagcgtg attactaaac atcctcacat tttccggcgt
    20941 aaagggaata cccaaccttt tcgcatcatc aaaaggacaa tccacctcca aaccagtatc
    21001 aactatcttt ttacaaatgc cttcttttaa cagataattc ttttctggat cttctcgata
    21061 ttctgcaatt aaatcgcgga tatttaccaa ttccttatac aaaccagcgc gtccataggg
    21121 agggacattg tgagaaatta acacgccata accgcgacgt ttggctaaaa ttgattctga
    21181 aggattattt gcggcataaa tgtagagatt gggcagattt cctaagagaa tatcagacca
    21241 agaataacca gtatttccta atggtgaacc tggcaaccat tcaacagttc cgtgcattcc
    21301 aaaatgaaca atagcatcag cttgaaaatc gttttgtaac catttataat aagctgcata
    21361 ttgaggatgg ggggttagat ctctttcaaa cattaaccgc atgggatctc cttgaattcc
    21421 caaaggtggc tggacaccta tccacacatt tcctaattgt acaccaccaa tattaaactc
    21481 gtctccatag gttttaatgc cgctaccagt cagatatttc cattgttttt caatgcgaga
    21541 tgtaaaaaga tagcctaacc atttttctaa tgttctagca ttaacagttg tgatgtcttt
    21601 tatgttattt tctcccaggg gattaatttc atctgctgct ttaatttggc gaattaattc
    21661 ttcaccatct tcgggaattt caccaactgt ataaccctgt gctttcagtg catgaagtaa
    21721 tttaattaaa ctgcgaggaa cgtttaataa tgcagcagtt cctacagcac cataaccagg
    21781 gggaaaacca tataaaataa tggcaatttt ccgttgagaa ataggttttt ggcgcaggga
    21841 aatccagctt ttaactctat tagttaatcg ctgtattcgt tcaggaacta aataaatttg
    21901 ttcccccact aatccaccga gaggaattgt atcaattgcg ccgtctaatt ctggtaaagc
    21961 atataagaca acactttgca gaccgcctac gccttgcctt gtccaagaat gaatatcttg
    22021 aattaataag ggtgcagcga cgatatacgg gacattttta gctgtgagaa tgcgtttagc
    22081 tacttccacc tgtcttccg
//
