LOCUS       NZ_CP051206            21914 bp    DNA     circular CON 09-DEC-2024
DEFINITION  Dolichospermum flos-aquae CCAP 1403/13F chromosome, complete genome.
ACCESSION   NZ_CP051206
VERSION     NZ_CP051206.1
KEYWORDS    RefSeq.
SOURCE      Dolichospermum flos-aquae CCAP 1403/13F
  ORGANISM  Dolichospermum flos-aquae CCAP 1403/13F
            Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Dolichospermum.
REFERENCE   1  (bases 1 to 5151752)
  AUTHORS   Wu,V.
  TITLE     Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial
            Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5151752)
  AUTHORS   Fomenkov,A., Anton,B.P. and Roberts,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2020) Genome Biology, NEB, 240 County Rd, Ipswich,
            MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-DEC-2016
            Assembly Method                   :: HGAP v. 2.3.0
            Assembly Name                     :: Afl_HGAP3_4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114.08x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012516395.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:24:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,921
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 66
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 35 of 187
            Pseudo Genes (incomplete)         :: 162 of 187
            Pseudo Genes (internal stop)      :: 14 of 187
            Pseudo Genes (multiple problems)  :: 21 of 187
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:01:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1529359
            Orig. end                         :: 1551273
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051206.1.
            Bacteria and source DNA available from CCAP 1403/13F.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21914
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="7"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21914
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21914
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11914](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11914)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     rRNA            1365..1482
                     /db_xref="RFAM:RF00001"
                     /gene="rrf"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="HGD76_RS07515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="HGD76_07515"
                     /product="5S ribosomal RNA"
     gene            1365..1482
                     /gene="rrf"
                     /locus_tag="HGD76_RS07515"
                     /old_locus_tag="HGD76_07515"
     gene            complement(1543..2367)
                     /gene="larE"
                     /locus_tag="HGD76_RS07520"
                     /old_locus_tag="HGD76_07520"
     CDS             complement(1543..2367)
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="larE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080313.1"
                     /locus_tag="HGD76_RS07520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07520"
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="WP_168695398.1"
                     /transl_table=11
                     /translation="MSWIEKLEQLKELFTEMEQALIAYSGGVDSTLVAKIAYDVLGDRA
                     LAVTAVSPSLLPEELEDAKIQAATIGISHKIVQTQEMDNPNYTSNPVNRCYFCKSELHD
                     TLKPLALELGYPYVVDGVNADDLHDYRPGIQAAKERGARSPLAEIGVTKAEVRQISQQL
                     GLPWWDKPAQPCLSSRFPYGEEITIAKLQRVGRAEIYLRNLGWQNLRVRSEGDTARIEL
                     SPDKIKDFVSNTDLPTLVSAFQTWGFIYVTLDLEGYRSGKLNQVLPQIALSK"
     misc_feature    complement(2539..2555)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
     gene            2717..3589
                     /locus_tag="HGD76_RS07525"
                     /old_locus_tag="HGD76_07525"
     CDS             2717..3589
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /locus_tag="HGD76_RS07525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07525"
                     /product="ISAs1 family transposase"
                     /protein_id="WP_233467087.1"
                     /transl_table=11
                     /translation="MQSLVEKLGVEVVAIDGKTHRGSYDRECKIKALHTVSAWASEHRL
                     ILGQTKVSCKSNEITAIPALLETLDLSGCIITIDAMGTQKSIAEQIIAGNADYILSLKD
                     NHPTLHQQVKNWFKTAQSLNFKGVDVSISQRVEKGHHRIENRTVYTVPISQLPALYEQN
                     QWAGLTTVVMVVRKVQHWNKTTHEIQFYITSLDSDANKIGSAIRQHWGIENSVHWTLDV
                     TFHEDECRIRSMHSPQNFALLRRIALNALDREPTFRRSIRQKSRRAAMNDQYMVSVLAA
                     SIANYSPLL"
     gene            3723..5045
                     /gene="trmFO"
                     /locus_tag="HGD76_RS07530"
                     /old_locus_tag="HGD76_07530"
     CDS             3723..5045
                     /EC_number="2.1.1.74"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="trmFO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080636.1"
                     /locus_tag="HGD76_RS07530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07530"
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="WP_168695399.1"
                     /transl_table=11
                     /translation="MTQQPIQVIGGGLAGTEAAWQIAQAGVPVILHEMRPKRFSPAHHT
                     ENLAELVCSNSFGAMASDRAAGLLHEELRQLGSIVISKADEHAVPAGGALAVDRGQFGE
                     DLTQTLANHPLIDFRRGEVKEIPQGIVVLASGPLTSPDLAADLQRFTGMEYLNFFDAAS
                     PIIVGDSINKDIAFMASRYDKGEAAYLNCPMNKEQYLQFRSALCQAEQTELKDFEKETA
                     KFFEACLPIEEMARRGEDTMRYGPLKPVGLSDPRNGERNYAVIQLRQEDKAGQLWNMVG
                     FQTNLRWGEQKRVFQMIPGLEKAEFVRLGVMHRNTFLNAPQLMSASLQFKERPTLLAAG
                     QLIGTEGYTAASAGGWLAGTNAARLALGKEPLILPITTMMGALFEFIRSAAPKHFQPMA
                     PNFGIVPDLGVKIKSKPEKYGRYRDRSLADLATWKMENLAV"
     gene            complement(5065..5358)
                     /locus_tag="HGD76_RS07535"
                     /old_locus_tag="HGD76_07535"
     CDS             complement(5065..5358)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214131.1"
                     /locus_tag="HGD76_RS07535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07535"
                     /product="hypothetical protein"
                     /protein_id="WP_041458064.1"
                     /transl_table=11
                     /translation="MKTRPGILLLTLAIPGFLVVLISLYYFGTDYDALIKAENYLEKLV
                     KEEKPNERTLQFAYHRALAHRINVFADATWGLLGGVITAVGIHGLVMLKEKD"
     gene            5633..6874
                     /locus_tag="HGD76_RS07540"
                     /old_locus_tag="HGD76_07540"
     CDS             5633..6874
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080633.1"
                     /locus_tag="HGD76_RS07540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07540"
                     /product="pitrilysin family protein"
                     /protein_id="WP_168697392.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 9.7e-25, bitscore:
                     85.5, seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M16_C (E-value: 2.5e-26,
                     bitscore: 90.9, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFPGSVIKLDNGLTFIHQEISTTPVVIADVWVRAGANLEPEPWFG
                     MAHFLEHMIFKGTPNLLPGEFDYHIEKIGGVSNAATSHDYAHYSITTAANYLEHTLPHL
                     GELLLNAAIPDDEFIRERDVVLEEIRSYNDDPDWVGFQSLVKNVYQQHPYGRSVLGTEP
                     ELMQHSPEAMRCFYRSYYQPENMTVVVVGGIGEKAARDLVNQTFVDFPQRDHCPQFTKI
                     AAPVISGIRRHELILPRLEQARLIMGWKVPGVEEFRAVHGLELLSALLGEGRISRLVSD
                     LREEKQLVQDLCCDFSLQQESSLFTITAWLESEYLERVEELICDHLQELQNQEISKPEI
                     NRIQRFLCNDYAFSTETPDQLTNFYGYYHTIANAELAVTYPQEIQSFNPQELQKLAQQY
                     LSPANYAVTILKPC"
     gene            6889..8160
                     /locus_tag="HGD76_RS07545"
                     /old_locus_tag="HGD76_07545"
     CDS             6889..8160
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080632.1"
                     /locus_tag="HGD76_RS07545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07545"
                     /product="pitrilysin family protein"
                     /protein_id="WP_168695400.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 1.1e-12, bitscore:
                     46.4, seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M16_C (E-value: 4.3e-38,
                     bitscore: 129.3, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTINSRSLIHRTVLSNGIVLLVSENQAADIIAGRIFIRAGSCHE
                     QREKAGLAHLLSAVMTKGCDGLSSLEIAEKVESVGAGLGTDAATDYFLLSLKTVTADFI
                     DILTLAGQLLRSPTFPENQIELEKRLAIQDIRSQKEQPFGLAFEQLRQVMYPNHPYSMS
                     VLGDETTMSSITRDDLVEYHQTHFRPDNIVISIAGRITPAQAEKLVNKIFGDWQIPNST
                     KPTLNLPPITGSPKSCLKPLNTQQSIIMLGYLGPSVTNPEYAALKLLATYLGNGLSSRL
                     FVELREKQGLAYDVSAIYSTRLFPASFVVYIGTAPENTKVAFNGLRQEVELLSTTELSA
                     EALQTAKNKIIGQYALGKQTNGHIAQIYGWYEILGLGIEFDQKFPELINNVTVTEAMTT
                     ACKYLQAPYLSLVGPEEAIQTATF"
     gene            8320..9090
                     /locus_tag="HGD76_RS07550"
                     /old_locus_tag="HGD76_07550"
     CDS             8320..9090
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080631.1"
                     /locus_tag="HGD76_RS07550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07550"
                     /product="peptidoglycan-binding protein"
                     /protein_id="WP_168695401.1"
                     /transl_table=11
                     /translation="MKFSLIASIPNYMSANSWYCLLLLTTTPALISSSAFISTAAAIKI
                     AQVNTEVKINRPPLNLGSQGERVTELQAALKLLGFYSGAVDGTYQQSTIIAVAQFQQAA
                     GLNPTGNVDNITWQKLFPSPSNIITSAPNAASSFTTVPTQPTRFTKPPTTKRTTNQPNS
                     SIQPTPLNQQMPGIKYTPQGWPILHLGTSGPEVIKLQKQLKILGFLNGKIDGDFGRSTE
                     AAVKAAQTRYGLKPDGVVGGGTWKVFLNRTSQSR"
     gene            9112..9465
                     /locus_tag="HGD76_RS07555"
                     /old_locus_tag="HGD76_07555"
     CDS             9112..9465
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996899.1"
                     /locus_tag="HGD76_RS07555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07555"
                     /product="phage holin family protein"
                     /protein_id="WP_015080630.1"
                     /transl_table=11
                     /translation="MKHFLLTWIGTAVALLITAKIVPGIILTSFVAALIAAVVIGLVNA
                     IVRPILQILAFPITLITLGLFTLVINALTLWFASAITPGYGFEIRGFIPAFLGSIVLSI
                     VSGIINQIVNAVD"
     gene            complement(9462..9887)
                     /locus_tag="HGD76_RS07560"
                     /old_locus_tag="HGD76_07560"
     CDS             complement(9462..9887)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080629.1"
                     /locus_tag="HGD76_RS07560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07560"
                     /product="cobalamin biosynthesis protein"
                     /protein_id="WP_168695402.1"
                     /transl_table=11
                     /translation="MVIKKLWVGIGCQKGISQQLINLVIKKVFQENQLIYHEIAGIATI
                     DKKASEIGLLEFCNLEKLPLKTFSAELLNNVFVPNPNNTITKLVGTSSVAEASAILAAS
                     EITSKEIMLLVPKQIFRLPEAPATITVAVAKSENLIF"
     misc_feature    9959..9974
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(10001..11914)
                     /gene="shc"
                     /locus_tag="HGD76_RS07565"
                     /old_locus_tag="HGD76_07565"
     CDS             complement(10001..11914)
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0016866 - intramolecular transferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080628.1"
                     /locus_tag="HGD76_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07565"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_168695403.1"
                     /sec_met_domain="T2TS (E-value: 2.2e-134, bitscore: 447.1,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTQDRIKVKQVVDAIAASQKHLLSIQNPDGYWWAELESNVTITS
                     EAVLLHKIWGTEKTRPLHKVENYLRSQQREHGGWELFYGDGGDLNTTVEAYMALRLLGV
                     PATDTALLKAKSLILAKGGISKTRIFTKLHLALIGCYNWRGLPSLPPWVMLLPDNFFFN
                     IYELSSWARSSTVPLLIVFDRKPIFNIDQPINLDELYAEGVNNVCWELPKNGDWSDIFN
                     ILDDGFKLAESINFVPFRNEGIKAAEKWILERQEVTGDWGGIIPAMLNSLLALKCLDYD
                     ANDPVIERGLKAVNNFAIETENSYCVQPCVSPVWDTAWAIRALIDSGFAPNDPAIVKAG
                     AWLIEKQILDYGDWNVKNKQGKPGAWAFEFENRFYPDVDDSAVVVMALHQAKLPNEELK
                     KQAIDRALNWIATMQCKPGGWAAFDLDNDQEWLNSVPYGDLKAMIDTNTADVTARVLEM
                     LGACNLSIQPDNLEKSLDYLLKEQEIEGCWFGRWGVNYIYGTSGVLSALALINPLKYAR
                     DINQGAAWLVKVQNSDGGWGETCFSYNDPSLKGKGDSTASQTAWALIGLIAAGEATDKL
                     AIDSIEKGINYLLETQKLDGTWDEEYFTGTGFPCHFYLKYHLYQQYFPLMALGRYQAKC
                     SS"
     gene            12068..13016
                     /locus_tag="HGD76_RS07570"
                     /old_locus_tag="HGD76_07570"
                     /pseudo=""
     CDS             12068..13016
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080627.1"
                     /locus_tag="HGD76_RS07570"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07570"
                     /product="glycosyltransferase"
                     /pseudo=""
                     /sec_met_domain="Glycos_transf_2 (E-value: 5e-12, bitscore:
                     44.2, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATIIFSLTLLSLVIWIFLLLFWGQFWRVNHQLEANKDKDIDNNI
                     LPTVCVIIPARNEADVIPVSLRSLLLQDYPGNFTIFLVDDQSSDGTANVAQEVAHNLDK
                     TQQLQIVTSTSLPPGWTGKLLSIFLGK"
     misc_feature    13507..13521
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 21.78"
                     /tool="antismash"
     misc_feature    complement(13993..14008)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            14190..17111
                     /locus_tag="HGD76_RS07575"
                     /old_locus_tag="HGD76_07575"
     CDS             14190..17111
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321388.1"
                     /locus_tag="HGD76_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07575"
                     /product="DUF4114 domain-containing protein"
                     /protein_id="WP_168695404.1"
                     /transl_table=11
                     /translation="MEPNINATTLADNTLIEDTTAIEDISSNVGITSEQDPTAIEDISS
                     NVATTSEQDPIPLENTTSIVDTTSEQDISSNVDISSNIDSTSTTNPTPIETSFLTLIDP
                     VTGETSLIDPVTGEIIPTVNKDGFVISGASDDQLGYSVSNAGDINGDGIGDVIIGSPLS
                     DPNDKFNSGKTYVVFGSKENFQQVIDPSNLNATTGFVINGAEVGDDSGRSVSNIGDING
                     DGVDDLVIGAPFSNINGDNSGAAYVVFGSKDPNHFSNPIELSNLEPSQGFTIKGNQSGD
                     LAGWAVSSAGDFNGDGVKDLLIGATHPSDDGTANKGEAYIIFGKEGGFDSSLNLANIDS
                     LGLKIISDNPNNLGYSVSEAGDINGDGLDDIILGAPSANTNGDNSGSSFVIYGRKTDPT
                     NPLTSKIINVSNLNSSDGFTINGQAGDQSGFSVSKAGDINGDGIGDLIIGAKDGNPNDK
                     ESAGRSYVVFGNKNGFGSGINLANLNGSNGFTINGVSALDNSGWSVSGLGDFNDDGIDD
                     IILGAKKATDNAGQGYIIYGSKQGFGATFELSTFDTPEYDGEKGFIIDGAENSNLGQSV
                     SGAGDVNGDGVNDLIIGAPLANSQAGEAYVIFGTPAATSAGTPDVNPTSTGTGTVSDFL
                     TLVSDNVFNIKGNNGKATLEVKLTGPNSGRNEIGVFTVDDASGKVDGIAPDEAGYAEKA
                     LAKGQVVFSTLTNLPAGFDPTSLDRLLRFNSNDNLRFYLVKDGSTDSVLSNNTSIGNVL
                     FANPANLKITDLGSDQYSLDWEDGSGNPSGFEDFPLTIQASDRPTALGTSLQNKPQGES
                     LDLRGITGAVTANFVVNREASYNNFVGFYRVTDANGGIDTNGDGKADILPGQDGYVQAA
                     INGRLSDINLAVNNGGTANFNNTLQGGSIYVPFLVVNGTPDALLDSNPNNNPDIYFTFL
                     GANSDGVDHVRMLGDNTFGFEDLRGGGDKDFNDVIVKVNLAVA"
     gene            complement(17147..17848)
                     /locus_tag="HGD76_RS07580"
                     /old_locus_tag="HGD76_07580"
     CDS             complement(17147..17848)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080624.1"
                     /locus_tag="HGD76_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07580"
                     /product="phosphorylase"
                     /protein_id="WP_168695405.1"
                     /transl_table=11
                     /translation="MVTILVPQGAEYQAVCRGLSRVPSSQAKVLAIPMGIEPVRKYLQQ
                     FTHGQANRMLIMGLCGSLSQKYQIGDIVLYQNCLYQGNLQEGNNSFTADIHNQLGDHVS
                     LVKGLTSDRLLYKATEKRQLYEQSGADVVDMEGYAFLEFFQQPVSPQVQIAILRVVSDD
                     AHHDIPNITSAIGADGSLQPLPLAWEFIRQPLAATRLITGSLKGLKTLTAVTQLLFTPN
                     KIPDFLEKSGI"
     gene            17939..20686
                     /locus_tag="HGD76_RS07585"
                     /old_locus_tag="HGD76_07585"
     CDS             17939..20686
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1049:
                     AcrB/AcrD/AcrF family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080623.1"
                     /locus_tag="HGD76_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07585"
                     /product="efflux RND transporter permease subunit"
                     /protein_id="WP_168695406.1"
                     /transl_table=11
                     /translation="MVKTNNSESARESFNISKLAIKFSWLTVCFWIGIAVAGVLALSSL
                     KYALFPDITFPVVVVNVQAPLTSALDTEEKLTKPLEESLKSLVGLEDIRSSTYPGQTAV
                     VSSFVVGTNLETATNKISQIVNQLNLPKNATKKIIPLNLNESAAVSYAIESSSGKIDNL
                     QQLVKDKIVPSIAKLPGVLKVSLLGEVSTNAPPPTATNGLPPASTLIRFNGKQALAFQV
                     IKKGDANTLEVVNRVEKEVQQLRSGLKDVTLTLAATQAEYIRNATHSTIDALIEAIILS
                     IVVIFPFLWNWQATLISALAIPISLLGTFIVMAIYGFNLETITLLALALVIGSIVDDAI
                     VDVENIMRHIEEGKPPREAALIATNEIGLTVTAATLTAVAVFLPIGLMGGVIGQFFKPF
                     GITVSAAMLTSMLVARTLSPVLAIYWLKAKPSSSPQKQSKIWLEFDQAYRNLLAWSLQH
                     RLIVVGLAISSLIAGLALIPLIPKGFIPKLDRGEFNIVYTAPLPSLPGDLAQGVQGGLG
                     QPGGQAGQLGQMGQKDKLGNSSLSAAALAASSSSIPFAIPLNDSLDIAKKLEEVVRRFP
                     AVATVFTTVGSREGEPNKGTLYVKLKAEREVKTAEVQDQLRAALPKLAGVTTSVEDIQF
                     VDTGGQKPLQIALQGNDIKALTTAAKAVKARIEKISGFADVTITGASHQQDGILQIERL
                     NNQRVVYIGANLGQNLTLGNATDQVVAEAKAVMPAGVSLNLGGDSARQNQVLGSFGTTL
                     GLSALCIIVVLIWLFKSWVDPIVIGLSLPLAVVGALLALLFTKSDFGMISLIGFVFLLG
                     ITNKNAILIVDYINQLRDSGLERTEAILKAGPVRLRPIMMTTAATILGMVPIALGLGAG
                     SELRSPMAVSIAGGLVSSTILSLFVVPVFYAILDDWFPRKTVKG"
     gene            20759..21655
                     /locus_tag="HGD76_RS07590"
                     /old_locus_tag="HGD76_07590"
     CDS             20759..21655
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080622.1"
                     /locus_tag="HGD76_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07590"
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="WP_168695407.1"
                     /sec_met_domain="PALP (E-value: 2.4e-10, bitscore: 38.4,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSIFFPPLIQQINSEICANADVKLFGLRLDLMHSQINGNKWFKL
                     KYNLVEAKERKLSTILTFGGAYSNHIYATAAAGNLFGFRTIGLIRGEENIPLNPTLEFA
                     VAQGMQLVYIDRQTYKKRHTEELQNQLKQRFGEVFMIPEGGCNLNGMRGCTEILQTVKG
                     FDTICLACGTGTTLAGMTLSLNQPQKVIGFPVLKGGDFLKEDINNLLTNYLASDLPTPV
                     NFPAPWQLISDYHFGGYAKVTNKLKLFCQDFQQQHDIPLDYVYTGKMFYGVMDLIAKGF
                     FQSESLLLIHTGGLQGN"
     gene            complement(21652..21912)
                     /locus_tag="HGD76_RS07595"
                     /old_locus_tag="HGD76_07595"
     CDS             complement(21652..21912)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308989.1"
                     /locus_tag="HGD76_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HGD76_07595"
                     /product="hypothetical protein"
                     /protein_id="WP_168642767.1"
                     /transl_table=11
                     /translation="MSRINPYTLQMQITQMFAQGQSFFALTKVQDWLKEHNQNPLDFEI
                     LFHKKPAPPGSKEVMVIEIELKRKDGQPVDPWLQEQANLHA"
ORIGIN
        1 gcaaaatggc cccgtaactt cgggagaagg ggtgcccacc tcagacgtgg gtcgcagtga
       61 agagatccag gcgactgttt accaaaaaca caggtctccg ccaactcgca agaggacgta
      121 tgggggctga cgcctgccca gtgccggaag gttaaggaag ctggtcagcg aaagtaaagc
      181 tggcgactga agccccggtg aacggcggcc gtaactataa cggtcctaag gtagcgaaat
      241 tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatctggatg gtgtctcaga
      301 gagagactcg gcgaaatagg aatgtctgtg aagatacgga ctgcctgcac ctggacagaa
      361 agaccctatg aagctttact gtagcctgga attgtgttcg ggcttcgctt gcgcaggata
      421 ggtgggaagc ggtgaactat tccttttggg gaatagggag ctaacggtga gataccactc
      481 tggcgaagct agaattctaa cccacgaccg tcagccggtt ggggaacagt ttcaggtggg
      541 cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcgcaaag gttccctcag
      601 cacgcttgga aaccgtgcga cgagtgtaaa agcaaaaggg agcttgactg caagactgac
      661 aagtcgagca ggtacgaaag taggctttag tgatccgacg gcgcagagtg gaatggccgt
      721 cgctcaacgg ataaaagtta ctctagggat aacaggctga tctcccccaa gagtccacat
      781 cgacggggag gtttggcacc tcgatgtcgg ctcatcgcaa cctggggcgg aagtacgtcc
      841 caagggttgg gctgttcgcc cattaaagcg gtacgtgagc tgggttcaga acgtcgtgag
      901 acagttcggt ccatatccgg tgcaggcgca agagtattga gaggagtcct ccttagtacg
      961 agaggaccgg gaggaacgca ccgctggtgt accagttatt gtaccaacag taaacgctgg
     1021 gtagccaagt gcggagagga taaccgctga aagcatctaa gtgggaagcc cacctcaaga
     1081 tgagtactct cactacaatt tgtaggtaag gtcacgggca gaacacccgt taataggctc
     1141 taagtggaag tgcagtaatg tatgaagctg aggggtacta atagaccgag ggcttgacct
     1201 ctattcaatt aaaaattgat aattaaaaat taaaaatgaa ggtttttggt ggttaattat
     1261 cactgttgat tctcgttctt tttgcagtct tcagggttgt gaatacccta agttaaaaat
     1321 cggtaattaa tttttaattc tgaattttta atttttaatt gtttcctggt gtctatggcg
     1381 cggtggaacc acactgatcc cttcccgaac tcagaggtga aacgctgctg cggcgacgat
     1441 agtttagggg tagccctccg tgaaaatagc tcgatgccag gtttattatt taacaacaat
     1501 gccccttcta ttcttttaga cggggttttg ttttttgggt tgttatttac tcaaagcgat
     1561 ttgaggtaaa acctgattta atttaccact acgataacct tctaaatcta aggtgacata
     1621 gataaatccc caagtttgaa atgcagatac tagagttggt aaatctgtgt tcgagacaaa
     1681 atctttaatt ttatctggtg ataattcaat tctggctgta tcaccttcgg aacggactcg
     1741 taaattttgc caccctaaat ttcgcaaata aatttcggct cttcctactc gctgcaattt
     1801 ggctatggta atttcttcac cgtagggaaa gcgcgaactt aaacagggtt gagcgggttt
     1861 atcccaccaa ggtaaaccta attgttggga aatttgccga acttcggctt tagtcacgcc
     1921 aatttctgct aaaggcgatc gcgcacctct ttcttttgca gcttgaattc cgggacgata
     1981 atcatgtaaa tcgtctgcat ttacaccatc taccacatag ggataaccta gttctaaagc
     2041 caaaggtttg agggtgtcgt gtaattcact tttacaaaaa taacaacgat ttactgggtt
     2101 agatgtgtaa ttaggattgt ccatttcctg tgtttggaca attttatgag atattccgat
     2161 agttgcggct tgaatttttg cgtcttctaa ttcttctggt aatagagaag gagaaaccgc
     2221 agttacagcc aaagcgcgat cgcctaaaac atcataagcg atcttagcca ctaatgtact
     2281 gtcaaccccc ccagaatagg ctattaaagc ctgctccatt tctgtaaata actcttttaa
     2341 ctgctctagt ttttctatcc aactcatctt cctaaaaccc cataacgcct aaccacttct
     2401 attttaatca ttagtcattc ttagatcacc aatattttca agaatttatc acctaatatg
     2461 tcaactacgg attacaagat caaaataagc aaactcaatt tatatttgat acagaggatg
     2521 attattaccc agggcaaacg cacctaaatt ctgtaatcca tgcccagcaa gggttttaac
     2581 ttttagttac aaaacttaac aatcgtcaaa acctttgtcc agtaagcatt ctaaaaataa
     2641 gatgcgcttg ccctgattta gaagagtatt tgagaaaata aaccccaaag aatttgagca
     2701 atgttttcgg aattgggtgc aatcattagt ggagaaattg ggtgtagaag tagttgctat
     2761 agatggtaaa actcatcgag ggtcatatga tcgagaatgc aaaataaaag ccttacatac
     2821 agtgagtgca tgggcaagtg aacatcggtt aattttaggg caaacaaagg tcagttgtaa
     2881 atcaaatgaa atcaccgcaa ttccagcact attagagact ctggacttat ctggctgtat
     2941 tatcactatt gatgcaatgg gtacacaaaa atcaattgcc gaacaaatca tagctggaaa
     3001 tgctgattat attttaagcc taaaagataa tcatcccacc ctacatcaac aagtgaaaaa
     3061 ttggtttaaa acagcacaat ctctaaactt taagggtgtt gatgttagta ttagtcaacg
     3121 ggtggaaaaa ggacatcacc gcatcgaaaa tcggacagtt tatactgttc caatttcaca
     3181 attaccagca ctttatgaac aaaaccaatg ggcaggatta acaacagtag tcatggtagt
     3241 tcgtaaggtt cagcattgga ataaaactac ccatgaaatt caattttata ttactagtct
     3301 tgatagtgat gctaacaaaa ttggtagtgc aattcgacag cattggggga ttgaaaactc
     3361 tgttcattgg acattggatg tcactttcca tgaagatgaa tgtcgaattc gttccatgca
     3421 cagtccacaa aactttgcct tactacgtcg tattgccctc aatgccttag acagagaacc
     3481 aacttttcgt cgcagtattc gacaaaaatc acgacgagct gccatgaatg atcaatacat
     3541 ggtttctgtg ttagcagcat ccatagcaaa ctattctcct ctactgtaat tcttctgtca
     3601 atagcgtttg agatgcgcta accctgcaaa attgatgaat ttaacaagag gattaagcat
     3661 ctaaaattct gatctgctct taattgttat tataaccaaa ccaagtaatt aaaaaaacaa
     3721 ctatgacaca acaaccgata caagtaattg gcggcggact ggcgggaaca gaagcagcat
     3781 ggcaaatagc tcaagctggt gtgcctgtga ttctccacga gatgcgccct aaacgcttca
     3841 gccctgccca tcatacggaa aatttggccg aattggtctg tagcaactct ttcggggcta
     3901 tggcgagcga tcgcgctgcc ggtctattac acgaagaact ccgccaactc ggttctattg
     3961 tcatctccaa agcagatgaa cacgcagtcc ctgctggtgg ggctttggcg gtagacagag
     4021 gacaatttgg cgaagatcta acccaaacct tagcaaatca tcctttaatt gatttccgac
     4081 gcggtgaagt taaagaaatt ccccaaggta tcgttgtatt agcttctgga cctttaacca
     4141 gtcccgattt agccgcagat ttacagcgat ttacggggat ggaatatctc aacttttttg
     4201 atgctgcgag tccaattatt gtcggagatt ctatcaataa agatattgca tttatggctt
     4261 ctcgttatga taaaggtgag gcagcttatc taaattgtcc gatgaataaa gagcagtatt
     4321 tacaatttcg atcagcactt tgtcaagcag aacaaacaga attaaaagac tttgaaaagg
     4381 aaacagcgaa attttttgaa gcttgtttac caattgaaga aatggcacgc agaggggaag
     4441 acaccatgcg ttatggaccc ctcaaacctg tgggtttatc tgatcctcgc aatggagaac
     4501 gtaactatgc ggtaattcaa ttacgacaag aagataaagc cggtcaactt tggaatatgg
     4561 tcggatttca aactaattta cgctggggtg aacaaaaaag agtttttcaa atgatacctg
     4621 gattggaaaa agccgaattt gtcagattag gagtaatgca ccgcaatact tttttaaatg
     4681 caccacagtt aatgtctgca agtttgcaat ttaaagaacg tccaacttta ctagctgcgg
     4741 gacaattaat aggaacagaa ggttatactg ctgcatctgc gggaggttgg ttagcgggaa
     4801 caaatgcagc tagattagct ttaggaaaag aacctctaat tttacctatt acaacgatga
     4861 tgggggcatt atttgagttt atcagatccg ctgcaccaaa acattttcaa ccgatggctc
     4921 ctaattttgg aattgtgcca gatttgggtg tgaaaattaa gagtaaaccg gaaaaatacg
     4981 gacgttaccg cgatcgctct ttagcagatt tagcaacttg gaaaatggaa aatttagccg
     5041 tttaaattaa taggaggaag tgttttaatc tttctccttc aacatcacta aaccatgtat
     5101 tcccactgct gtaatcacac ctcccaataa tccccaagta gcatcagcaa aaacattaat
     5161 tcgatgggct aaagctcgat gatatgcaaa ttgcaaagtt ctttcattag gcttttcctc
     5221 ttttaccaat ttttctaaat aattttcagc ttttattagt gcgtcataat cagtaccaaa
     5281 ataataaagg gatatcaata ctaccaagaa accagggatg gctaaagtaa gtaataaaat
     5341 tcctgggcgt gtcttcataa aaacgatatt attaaatacc aactaacaac tgatgaaaaa
     5401 ggcaagctga ctgctactaa cgcatctatc gaggagaaaa tgccagcgtg gatagtcgtt
     5461 tttctcattc tatcaatatt acctatttgg ggaacattat ttatccataa aacttgttga
     5521 tttactaaat ttctacaatt tggctttctt ttccgttaaa ttcaaggtta gaatgacaaa
     5581 attaaattgt ggttgaaact agaaaaaatt gctaaaacaa ctaactaaca acgtgtttcc
     5641 cggttcagtt atcaaattag ataacggctt gacattcatt caccaagaaa tatccactac
     5701 acccgtagtc atagctgacg tttgggtgcg tgcgggagct aacctagaac cagaaccttg
     5761 gtttggaatg gcacattttc tagaacacat gattttcaaa ggtacaccaa acttgctgcc
     5821 tggagaattt gattatcata ttgaaaaaat tggtggtgtg agtaatgccg ctaccagcca
     5881 cgattatgcc cattattcga ttacaaccgc tgctaattac ctagagcata ctctaccaca
     5941 tttgggagaa ttactcctga atgcagccat tcctgacgat gaatttatca gagaacggga
     6001 tgtggtgcta gaagaaatcc gttcttacaa cgatgatcct gattgggtag gttttcaatc
     6061 tcttgttaaa aatgtttacc aacagcaccc ttacggacgt tctgtattgg gaactgagcc
     6121 agaattaatg cagcattccc ccgaagctat gcgctgtttt tatcgcagtt attaccaacc
     6181 agaaaatatg actgtggttg tggtgggggg aattggtgaa aaagctgcga gggacttggt
     6241 aaaccagaca tttgtagatt ttccgcaacg tgatcattgt ccccaattta ccaaaattgc
     6301 agcaccggta atttcaggaa ttcgtcgtca tgagttaatt ttacctagac tggaacaagc
     6361 gcggttaatt atgggttgga aagtgccagg agtagaagaa tttcgggctg ttcatggttt
     6421 agaattatta tcagcactgt taggagaagg gagaatttct cgattagtat ctgatttgcg
     6481 ggaagaaaag caacttgttc aagacttatg ctgtgatttc tctttacaac aagaatcaag
     6541 tttatttaca attacggctt ggttagaatc agaatatctg gaacgcgttg aagagttgat
     6601 ttgtgatcat ttacaagaat tgcaaaatca agaaattagc aagccagaaa taaatcgtat
     6661 tcagaggttt ttatgtaatg attatgcctt ttctacagaa acaccagatc agctaactaa
     6721 tttttatggt tattatcata ccattgctaa cgctgaatta gctgttacct atccccaaga
     6781 aatccagtct ttcaatccgc aagaactgca aaaattagct caacaatatc tttccccagc
     6841 taattatgct gtgacaattc tcaaaccttg ttaattatta agtaaaaaat gcaaactatt
     6901 aatagtcgct ctctgattca tcgcaccgta ttaagtaatg gcattgtttt attagtctca
     6961 gaaaatcaag ctgctgatat tattgcggga cggattttta ttcgcgctgg tagttgtcat
     7021 gaacagcgag aaaaagctgg tttagcgcat ttattatcag cagtaatgac aaagggatgt
     7081 gatggtttat cgagtttaga aattgctgaa aaagttgaat ctgtaggagc tggtttaggt
     7141 acagatgcag ctacagatta ttttttgcta tcgttgaaaa cagtaactgc tgattttatt
     7201 gatattttaa cattggctgg acaattattg cgatcgccta cctttcccga aaaccaaata
     7261 gaattagaaa agcgattagc aatccaagat attcgttccc aaaaagagca accttttgga
     7321 ctggcttttg aacaactgcg tcaggtaatg tacccaaatc atccctactc tatgtcagtt
     7381 ttaggtgatg aaacaaccat gagcagcatt acccgtgatg acttagtaga atatcatcaa
     7441 actcatttcc gtcctgataa tattgtcatt agcattgctg gacgcattac accagcacaa
     7501 gcagagaaat tagtaaataa gatttttggt gattggcaaa taccaaattc caccaaacct
     7561 actcttaatt taccaccaat tactggatca cctaaatctt gcctgaaacc cttaaataca
     7621 caacaatcaa ttatcatgtt gggttattta ggaccatcag taaccaaccc agaatatgca
     7681 gccctcaagt tacttgctac ctatttaggg aatggtcttt ctagtcgttt atttgtagaa
     7741 ttgcgagaaa agcaaggttt agcttatgac gtatctgcta tttattcgac tcgacttttt
     7801 cccgcttcct tcgtggttta tattgggaca gcaccggaaa acaccaaagt tgcttttaat
     7861 ggacttcgcc aagaagtaga attattatct accaccgaac tgtcagcaga agcattacaa
     7921 actgctaaaa acaaaattat cggtcaatat gcgttgggta aacaaactaa tggacatatt
     7981 gcacagatat atggttggta tgaaattttg ggtttaggaa ttgaatttga tcaaaagttt
     8041 cccgaattaa ttaataatgt cactgtaacg gaggcaatga caaccgcttg taaatattta
     8101 caagcaccat acttatctct cgttggacca gaagaagcca ttcagacagc tactttctaa
     8161 aaccttaaat tgcgtaggtt gggttgacgt aaggaaaccc aacaaaatca agtcttggaa
     8221 atattcgatt atgttttatc ttcacaccaa aaagattaaa ggcaaacaaa gcgatcgcca
     8281 cagggtaaga tcagcatcgg aaagttatac aaaaattcta tgaaatttag tttgatagca
     8341 agtattccca actacatgag cgcaaattct tggtattgct tactcttgtt aaccaccaca
     8401 cccgcgttaa ttagttcctc tgcttttata tcaacagcag cagcgattaa aattgcccaa
     8461 gtcaacacag aagttaaaat taatcgtcct cctctaaact taggtagtca gggtgaacga
     8521 gttacagaac ttcaagccgc tttgaagctt ttgggctttt attcaggtgc tgtggatggt
     8581 acatatcaac aaagtaccat tattgccgtt gcccaatttc agcaagctgc tggcctaaat
     8641 cccactggta atgtagataa tatcacttgg caaaaactgt ttcccagccc atcaaatatt
     8701 attacttccg cacctaatgc tgccagtagt tttacgactg ttcctaccca accgaccaga
     8761 ttcacaaaac caccgactac taaacgcacc accaatcagc ctaattctag tattcaaccc
     8821 actcctctca atcaacaaat gcccggtatt aaatatactc cccaaggatg gccaatttta
     8881 catttaggta caagtggacc ggaagtaatt aaattgcaaa aacaactcaa aatcctgggt
     8941 tttttaaatg gtaaaataga tggagatttc ggaagatcca cagaagccgc agtcaaagct
     9001 gctcaaactc gttatggttt aaaacctgat ggtgttgtcg gtggtggtac atggaaagtg
     9061 ttcttgaacc gcacttccca gtcacgttaa tcttgttctc gtcatcaaac tatgaagcac
     9121 tttcttttaa cttggatcgg gactgcggta gcattattaa ttacggctaa aattgttcct
     9181 ggaatcattc tcactagttt tgttgctgcc ttaattgccg ctgttgtgat tggtttagta
     9241 aatgccattg tgcgaccaat tttgcagatt ttagcatttc ccatcacctt aattactttg
     9301 ggtttattca ccctggtgat taatgcctta acgctatggt ttgccagtgc cataactccc
     9361 ggctatggtt ttgaaattcg gggttttatt cctgcttttt taggttcaat tgttttatca
     9421 attgtttctg gtattattaa tcagattgtt aatgcagttg attaaaatat caggttttca
     9481 cttttagcaa cggctactgt gattgttgct ggtgcttctg gtaatcggaa aatctgttta
     9541 ggaactaata acattatttc cttagatgtg atttctgaag ctgcaagaat cgcggaagcc
     9601 tctgctacgc tagatgttcc cactaactta gtaatagtat tattaggatt gggaacaaat
     9661 acattattta aaagttccgc actaaaggtt tttaaaggta gtttttctaa gttacaaaat
     9721 tccaataaac caatttctga ggctttttta tcaatagttg caattccagc tatttcatga
     9781 tatataagtt gattttcctg aaaaactttt ttgattacta aattaattaa ttgttgggaa
     9841 attccttttt gacaaccaat tcctacccat aattttttta taaccatatt atccactttc
     9901 tttgacaaca ctaataaatg gggtttagaa atagcgggca agatgcccac actacaagat
     9961 tgataatatt tctccagaaa aatggcaaag gtattgctat ttaactcgaa cattttgctt
    10021 gatatcgtcc caaagccatt aaaggaaaat actgctgata aaggtgatac ttcaaataaa
    10081 aatggcaagg gaagcccgtt ccggtaaagt attcttcatc ccaagtacca tctaattttt
    10141 gtgtttctaa taggtaatta atcccctttt caatactatc aatcgctaat ttatccgttg
    10201 cttcacctgc tgctattaac ccaattaaag cccatgctgt ttgggatgct gtactgtcac
    10261 cttttccttt taaactagga tcattgtaac taaaacaagt ttctccccaa ccaccatcag
    10321 aattttgtac tttcactaac caagctgcac cctgatttat atctctagca tatttcaaag
    10381 gattaattaa agccaaagct gataaaacgc cgctagttcc ataaatgtaa tttacacccc
    10441 aacgtccaaa ccaacaacct tctatttctt gttctttcaa aagataatct aaagactttt
    10501 ctaaattatc tggttgaatt gataaattac aagcccccaa catttccaat actctagcag
    10561 tgacatcagc cgtatttgta tcaatcatcg ctttcaaatc accatagggg acagaattga
    10621 gccattcttg atcattatca agatcaaaag ccgcccaacc acctggttta cattgcatcg
    10681 tcgcaatcca atttaaagcc ctatcaatag cttgtttttt tagttcctca tttggcaatt
    10741 ttgcttgatg taaagccatg acaaccacag ccgaatcatc cacatctggg taaaaacgat
    10801 tttcaaattc aaacgcccaa gcaccgggtt ttccctgctt gtttttgaca ttccaatcac
    10861 cataatcgag aatttgtttc tctattaacc atgctccagc tttaacaata gccggatcat
    10921 ttggggcaaa acctgaatca atcaaagccc gaatcgccca agctgtatcc catacaggag
    10981 aaacacaagg ttggacacag taactatttt ctgtttcaat ggcgaaatta ttaactgctt
    11041 ttaaaccccg ttcgatcacc ggatcattag catcataatc taaacacttt aaagctaaca
    11101 gagaatttaa cattgcggga ataattcccc cccaatcacc tgtaacttct tgacgttcta
    11161 gaatccattt ttctgcggct ttaattcctt catttctaaa agggacaaaa ttaatacttt
    11221 ctgctaattt aaagccatca tcaaggatat taaaaatatc tgaccaatca ccattttttg
    11281 gtaattccca gcagacatta tttacacctt cagcatataa ttcatctaaa ttaattggct
    11341 gatcaatgtt aaaaattggt tttcgatcaa agacaattaa taatggcaca gtgctagaac
    11401 gcgcccaact cgaaagttca taaatattga agaaaaaatt atcgggtagt aacattaccc
    11461 aaggtggcaa agaaggcagt ccgcgccaat tataacagcc aattaaagct agatgtaact
    11521 ttgtaaaaat ccgagttttg ctaataccac ctttagctaa aattagagat ttcgctttca
    11581 gcaatgcggt atctgtagcg ggtacaccta acaatcttaa agccatatag gcttctacag
    11641 ttgtgtttaa atctccacca tcaccataaa acaattccca acctccatgt tctcgttgtt
    11701 gagaacggag ataattttca actttatgta aaggtctggt tttctcagtt ccccaaatct
    11761 tatgtaataa aacagcttct gaagtgatgg taacattaga ctctaactca gcccaccaat
    11821 aaccatcggg attttgaata gaaagtagat gtttttgact tgcggctatc gcatcaacaa
    11881 cttgcttgac ttttattcta tcttgtgttt gcatcaaata cctacctcag caccaaacaa
    11941 tatttaaata acataaccga attacgagaa aattcttccg caaaatcagc aaacattgta
    12001 ttctatccaa gtcagtaaaa gaattaaatt tgtcttttaa attttaaatt ggagtacagc
    12061 aacagttatg gcaacaatta tattcagttt gacccttcta tccttagtca tttggatatt
    12121 tttactctta ttttgggggc agttttggcg agttaatcac caattagaag caaataaaga
    12181 taaagatatt gataacaaca tcttaccgac cgtgtgcgta ataattcctg cccgtaatga
    12241 agccgatgtc attcctgtta gcttgcgatc gctcctcctt caagactatc ctggtaattt
    12301 caccatcttt ttagtagacg atcaaagtag cgatggtaca gccaatgttg cccaagaagt
    12361 agcccacaac ctcgacaaaa cccagcaatt gcaaattgtt accagcacct cattaccccc
    12421 aggttggaca ggcaaattgt tatccatttt cctgggtaaa taatcccaaa aaagctactg
    12481 ctggcgctgc tggaggttgt attctcattc actcagaagc cctaaataga attggtgggt
    12541 tgcaaataat tcgtcaagcc ttaattgatg attgttccct agccaaagcc gttaaatcaa
    12601 ctcacggaaa aatctggcta ggattaagca ccctaaccta tagcttacgt ccttacgact
    12661 ccctgaaaac aatttgggat atggtcgcca gaagtgctta cacacaacta aattattccc
    12721 ctttcctgct aataggcagc ctgttgggaa tgactttagt ttatttatta ccaccgatag
    12781 caattatttt cggtctggta ttggggaatt ggataattac cctgatttct ttcatcggat
    12841 atctactgat gacttttgct tatttcccaa ttattcgttt ttataaatgt tctccagttt
    12901 ttgcttttag cttacccatc attgcttttt tatatacttc tatgacaata gattcagcat
    12961 tacaacattg gcaaggacgt ggaggtgcat ggaaaggtag agtatatacc cgttgacaag
    13021 ataagagaag caagagaagc agagaaagaa atttccttct tcttccttct tcttccttcc
    13081 tcctgatagc gaagcgtggc gttagccata ctcctgatag cgaagcgcgg cgttagccat
    13141 actcctgaac tccttcttcc cgagtttacc ccgttgacaa ccagtagcag acattttttc
    13201 cccaaattcc cattctcacc cattaaacac cagccccagc attacccaca cattaggatt
    13261 actggtaaag acagtagatc aaaagtagtc agaatattta aaagaagtcc ggattggaca
    13321 ttagcaatta gggattaagt tataggaagc gggacttgag aacagggaag agaaattttt
    13381 tccattaaca tcaagtatca taccgcagac taaatttgat tgtcatgttt acagccaatg
    13441 ataaaagcca aactcagaaa tttgcgtgtt attgacctca agttcacaga agcttcacaa
    13501 ctaatagcac aatatctagg ctgtactgtt ttcgggcaat caaagtaaac tttctcgtta
    13561 gtgaaaagct aagagtcctt aaatcaaatc aggtataaac ttcagcatct tccttcgttt
    13621 gggtgtcgtc ggcaaaaacc caagtcttaa atagacctgg atttgatcat aagaggtgaa
    13681 aacttttgta tataagatgg atgatgaagg tttgatctcc catagaagtt aaccggatct
    13741 gaccatctca aaaaaaatta tctataccaa ctctggtaac acaactacag tccagagaaa
    13801 tttcagcata ccagggcatt aactactatt cccctatttc catcctcagt tactctaatg
    13861 actcaaactt agaaacaaac acgcatacag cacttcccgg tgttatgagg tacatatcta
    13921 gcgggcaaga tgcccgcact acaagagttt catgattcaa ctttgtacct cataagagcg
    13981 gaaaccgctg tatttacccc aggggtatga aattctatcc attattatca tgaacaccaa
    14041 ctctagcaac tcaactacag cctagagcta tttcttaacg cagatttata gcaaatgttt
    14101 gtgtaactaa ggtggaacaa cccattacac ctgctttgct gtaaccgaaa tttatcaaac
    14161 attcaaaaaa ccaataacac tacaccatca tggaaccaaa tattaacgct actacactcg
    14221 cagataacac tctcattgaa gacaccactg ccattgaaga tatcagttct aatgtaggca
    14281 tcacctccga acaagatcct actgccattg aagatattag ttctaatgta gccaccacat
    14341 ccgaacaaga tcctatccct cttgaaaata ccacctccat tgtagacacc acctccgaac
    14401 aggatattag ctccaatgta gatattagtt ccaacataga tagcacttcc accacaaatc
    14461 ccacacctat tgaaacctca tttctaaccc ttatagatcc tgtaactggc gagactagcc
    14521 ttatagatcc tgtaacgggc gagattatac ccacagtcaa caaagatggc tttgtcatca
    14581 gcggtgcaag tgatgatcaa ttaggctact cagttagcaa cgccggtgat atcaacggtg
    14641 atggcattgg ggatgtaatc atcggctcac ctttgagtga tcctaatgac aaatttaact
    14701 ccgggaaaac ttatgtagtc tttggcagca aagagaactt tcaacaagtc atagaccctt
    14761 ccaatctcaa tgctactaca ggctttgtga ttaatggggc agaagttggt gatgactcag
    14821 gccgttccgt tagcaatatt ggggatatta acggtgacgg tgttgatgat ctagtcattg
    14881 gcgcaccctt ttctaacatc aatggggata attccggggc tgcttatgtc gtctttggca
    14941 gtaaagaccc caaccatttt agtaatccca ttgaactatc caaccttgaa cctagccagg
    15001 gttttaccat caaaggtaat cagtctggtg atcttgcagg ttgggctgtt agcagtgccg
    15061 gcgattttaa tggtgatggc gttaaagacc tattaattgg tgcaactcat cctagtgatg
    15121 acggaacagc taataaagga gaagcctata tcatctttgg gaaagaaggt ggttttgatt
    15181 ccagcttaaa ccttgctaat attgacagct taggcttaaa aatcatcagt gataacccaa
    15241 ataacctggg ctattccgtt agtgaggccg gagatatcaa cggtgatgga cttgatgaca
    15301 tcattcttgg tgcgccctct gctaatacca atggtgataa ttctggaagc agttttgtta
    15361 tttatggacg gaagactgat cccacaaatc cccttacttc caagattatc aacgtctcta
    15421 acctcaatag cagcgatggt tttaccatca acggacaagc cggtgatcaa tcaggcttct
    15481 ctgttagcaa agctggagat atcaacggtg acggcatcgg agacttgatt attggggcaa
    15541 aagatggcaa tcccaatgac aaagaatctg ctggcagaag ctatgtagtc tttggtaata
    15601 aaaatggttt tggttctggc atcaatcttg ctaaccttaa tggtagcaat ggctttacca
    15661 tcaacggtgt ttccgccctt gacaactccg gctggtctgt cagtggtttg ggagatttta
    15721 acgatgatgg tattgatgac atcattcttg gggcaaaaaa ggctactgat aacgctggac
    15781 aaggctatat tatttatggc agcaaacaag gctttggcgc tacctttgaa ctctcaactt
    15841 tcgatacacc tgagtacgat ggtgaaaagg gctttatcat tgatggtgct gaaaatagta
    15901 acttaggtca gtcagtcagt ggtgctggag atgtcaatgg tgatggtgtt aatgatttga
    15961 ttattggcgc tcccttggct aattctcaag caggtgaagc ctatgtgatc ttcggaactc
    16021 ctgctgccac atctgctggt acacctgatg ttaatccaac ttccactggc accggcactg
    16081 ttagcgattt cctaacgcta gttagtgata atgtcttcaa tatcaaaggc aacaacggca
    16141 aggcaacact ggaagtaaaa ttaacaggac ccaattccgg tcgcaatgaa attggagtat
    16201 ttactgttga tgatgcgtct ggtaaagttg atggtattgc ccccgatgaa gcgggttatg
    16261 cagaaaaagc attagcaaaa ggtcaggtag ttttctctac ccttactaat cttcctgctg
    16321 gatttgatcc tactagcctg gacaggttat taagatttaa ctccaatgac aatttgagat
    16381 tctacttggt taaagacggt tcaaccgatt ctgtactgag taataacacc tctattggca
    16441 atgtcctgtt tgccaaccct gctaatctca agattacaga tttgggatct gatcagtatt
    16501 ccctagattg ggaagatggt tctggtaatc ctagcggctt tgaggatttt ccgttaacaa
    16561 ttcaggccag tgatcgacct acagctttgg gtacatcttt gcaaaataaa ccacaaggcg
    16621 aatcgctgga tttacgaggt attactggcg cagtaaccgc taattttgtg gttaaccgag
    16681 aagcaagtta caacaatttt gttggtttct atcgcgtgac tgatgcgaat ggcggtattg
    16741 atactaatgg tgatggtaag gctgatatcc tcccaggaca agatggttat gttcaagcag
    16801 ctattaatgg tcgtctttct gacattaatt tagctgtgaa caacggtgga acggcaaatt
    16861 tcaataatac gctccaaggt ggctctatat atgtaccatt tcttgttgtg aatggtacac
    16921 ctgatgccct acttgatagt aatcctaata ataatccaga tatttacttt accttcttgg
    16981 gtgcgaactc agatggtgta gatcatgtgc ggatgttagg tgataatacc tttggttttg
    17041 aggatttacg tggtggtggt gataaagatt ttaatgatgt aattgttaaa gtcaatttgg
    17101 ctgtagcgta aaagatattt ctcaggtttg agaatattca tcagaatcag atccccgact
    17161 tctctaagaa gtcggggatc ttgttaggag taaataataa ttgtgtgact gctgttaaag
    17221 ttttgagtcc ttttagtgaa cctgtaatta atcgagtcgc tgctaggggt tgacggatga
    17281 attcccaagc taagggtaaa ggttgtaatg aaccgtctgc accaatggct gatgtgatat
    17341 tggggatgtc atgatgagca tcatcgctaa ctactcgcag gatggctatt tgtacctgtg
    17401 gagaaactgg ttgctggaaa aattctagaa aagcgtatcc ttccatatct acaacgtcag
    17461 caccggattg ttcatataac tggcgttttt ctgtggcttt atataagagg cgatcgctcg
    17521 ttaagccttt gactaaagat acatgatcac caagttgatt atgtatatct gcggtgaagg
    17581 aattattgcc ctcttgcaga tttccttgat aaagacaatt ttgatataaa acaatatctc
    17641 caatttgata tttttggctc aagctaccgc acaaacccat gatcagcatc cgatttgctt
    17701 gaccatgggt gaactgttgt aaatatttcc gcactggttc gattcccatt ggtatcgcta
    17761 ataccttggc ttgggaactg gggacgcgac ttaatccgcg acagacggct tgatattctg
    17821 ctccctgggg gactaagatc gtaaccattg ttatcctaga agttctatac taacctgtta
    17881 gttgtcagtg atcaaaagac cgatgactaa tgaccaagga cttaaataat tttatcagat
    17941 ggtaaagact aataactcag aatctgcccg cgaaagcttc aatatttcca aattagcgat
    18001 taagttttct tggttgacgg tgtgtttttg gataggtata gcggtagctg gtgtcctggc
    18061 cttgagttcc ctcaaatatg ctttatttcc agatatcacc tttccggtag tggtggtaaa
    18121 tgtccaagca cctttaacat cagccctaga tacggaagaa aaactcacta aaccactgga
    18181 ggaaagtctc aaatcactgg tgggacttga ggatattcgt tcatcaactt atcctggtca
    18241 aactgctgta gtttcctctt ttgtggtggg tacaaattta gaaactgcga ctaataaaat
    18301 ttctcagatc gttaatcaat taaatctacc taagaatgcc actaaaaaaa tcatcccttt
    18361 gaatctgaac gaatcagcgg ctgttagcta tgccattgaa agttcttctg ggaagattga
    18421 taatttacag caactggtaa aagataaaat tgtccctagt atagctaaat taccaggagt
    18481 tctgaaagtc tctctgttag gggaagtgag tacgaatgca ccaccaccaa cagcaaccaa
    18541 tggattaccc ccagccagta ctttaatcag gttcaatggt aaacaggctt tagcctttca
    18601 ggtcattaaa aaaggtgatg ctaatacctt agaagtagtt aatcgggtag aaaaagaagt
    18661 acaacagcta aggtctggct taaaagatgt tactctcacc ttagctgcta cccaagctga
    18721 atatatccgc aacgccaccc attctaccat agatgccctg atcgaagcga taattttatc
    18781 aattgtcgtc atctttccct ttttatggaa ttggcaagct accctaattt ctgccttagc
    18841 tattcccata tctttgctag ggacatttat cgtcatggcg atatatggct ttaacttaga
    18901 aactatcacc ctgttagcct tagctttagt cattggctcg atagtagatg atgccatagt
    18961 tgatgtagaa aacatcatgc ggcatattga agaagggaaa cccccccgcg aagccgcttt
    19021 gatagccact aatgaaattg gtttaactgt caccgcagcc actttgacag cggtagcagt
    19081 atttttgcct attggtttaa tgggtggagt cattggtcag tttttcaaac cctttgggat
    19141 taccgtttct gctgccatgc tgacatctat gttagtagcc cgaaccttat caccggtctt
    19201 agctatttat tggcttaaag ctaaaccatc cagttctcct cagaagcaaa gtaaaatttg
    19261 gctagaattt gatcaagctt atcgtaactt gttagcctgg tctttgcagc accgcttgat
    19321 agttgttggg ttagctataa gtagtttaat tgctggtctt gccctgattc ccctaattcc
    19381 caaaggattt attcctaaac ttgaccgagg agaatttaat attgtctata ctgctcctct
    19441 acctagtctt cctggggatc ttgcacaagg ggtacaagga ggactaggac aaccaggagg
    19501 acaagcagga caactgggac aaatgggaca aaaagataaa ttaggaaatt cgtctttatc
    19561 tgctgcggct ttggctgctt catcatcttc tattcctttt gctattcctt taaacgattc
    19621 tttggatatt gctaagaaac tggaagaagt agtgagaagg ttccccgcag tggcaacggt
    19681 atttactact gtgggttctc gtgaaggtga accaaacaag gggacgctat acgttaagct
    19741 caaagcagag cgagaagtga aaacggcaga agtacaagac caactccgtg ccgctttgcc
    19801 aaaattggcg ggtgtaacca caagtgtaga ggatattcaa tttgtagaca ccggtggaca
    19861 aaaacctctg caaatagctt tgcaaggtaa tgatatcaaa gctttgacga cagcagctaa
    19921 agcggtaaaa gctcgaattg agaaaatatc tggttttgct gatgtgacta ttacaggtgc
    19981 gtctcatcaa caagatggga ttttgcaaat tgaaaggttg aataatcagc gagtagttta
    20041 tatcggtgct aacctgggtc aaaatttaac tttaggtaat gcaactgatc aagtcgtagc
    20101 tgaagctaaa gctgtgatgc cggctggtgt gtctttgaat ttagggggag attctgcccg
    20161 tcaaaatcag gttcttggga gttttggcac aactttggga ttatcggcac tttgtattat
    20221 tgtggtgcta atttggttgt ttaaaagttg ggtagatccc atagttattg gtctttcttt
    20281 acctttagca gtggttgggg cgttgctggc actgctattt acaaaaagtg attttgggat
    20341 gatatctctg attggttttg tgtttctgct ggggattact aacaaaaatg cgattttaat
    20401 cgtagattac attaatcagt tacgggattc tggactagaa cggactgagg caattcttaa
    20461 agctggacct gtacgtttac gtcccattat gatgacaact gcggcaacaa ttttagggat
    20521 ggttcctatt gctttgggtt taggtgcggg ttcggaattg cgatcgccta tggctgtatc
    20581 tatagctggt ggtttagtaa gttctactat tctcagcttg tttgttgtcc cggtattcta
    20641 cgccatttta gatgattggt ttccgaggaa gacggtgaaa ggataaatct ttcagatccc
    20701 cgatttttta tctaagtcgg ggatcttagt attttataaa gcttaacttt tcctgaaaat
    20761 gtcgtcaatt ttttttcctc cacttattca acaaattaat agtgaaatct gtgctaatgc
    20821 agatgttaag ttattcggat tacgcctgga tctcatgcac tcccagatca acggtaataa
    20881 atggtttaag ctgaagtata acctggtaga agctaaagag aggaaattat cgacaattct
    20941 cacctttggt ggtgcttatt ctaatcatat ttatgctacc gctgccgcag gaaatctgtt
    21001 tggttttcgc actattggct taattcgtgg agaagaaaat attcctttaa atcctactct
    21061 agaatttgca gtagcacagg gaatgcaatt agtatatatt gatcgtcaga cctataaaaa
    21121 acgccataca gaagaattac aaaatcagtt aaaacagcga tttggggaag tatttatgat
    21181 tcctgaaggg ggttgtaatt taaatggtat gcgtggctgt acagaaatat tacaaacagt
    21241 taaaggattt gatactatct gtttggcttg cggtacagga acaacattag ccgggatgac
    21301 actatcatta aatcaaccac aaaaagtaat tggttttcct gtgttgaaag gtggtgattt
    21361 tctcaaggaa gatatcaata acttattaac aaattatcta gcttctgatt taccaacacc
    21421 tgttaatttt cctgcacctt ggcaacttat atctgattat cattttggtg gttacgctaa
    21481 ggtgacaaat aaattaaagc tattttgtca agattttcaa caacaacatg acataccttt
    21541 agattatgta tacacaggaa aaatgtttta tggagttatg gatttaattg caaaagggtt
    21601 ttttcaatcg gaatctttgc tattaatcca tactgggggt ttacagggga attaagcgtg
    21661 taaattagcc tgttcttgta accaaggatc tacaggttgt ccgtctttgc gtttaagttc
    21721 tatttctatt accatgactt ccttagaacc gggaggtgcg ggttttttat gaaagagaat
    21781 ctcgaaatct aaggggtttt gattatgttc ttttaaccaa tcttgaactt tggttagggc
    21841 aaaaaatgat tgtccttggg caaacatttg ggtaatttgc atttgtaagg tataaggatt
    21901 aatgcgactc attt
//
