LOCUS       NZ_JAAVIO010000013     10324 bp    DNA     linear   CON 03-AUG-2024
DEFINITION  Bacillus toyonensis strain M5 contig13, whole genome shotgun
            sequence.
ACCESSION   NZ_JAAVIO010000013
VERSION     NZ_JAAVIO010000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 134530)
  AUTHORS   Huang,Y., Palmer,J.S., Ding,Y. and Yu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) School of Food and Advanced Technology,
            Massey University, Private Bag 11222, Palmerston North,
            Manawatu-Wanganui 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012395365.1-RS_2024_08_03
            Annotation Date                   :: 08/03/2024 02:52:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,219
            CDSs (total)                      :: 6,078
            Genes (coding)                    :: 5,835
            CDSs (with protein)               :: 5,835
            Genes (RNA)                       :: 141
            rRNAs                             :: 17, 6, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 7, 6, 13 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 103 of 243
            Pseudo Genes (incomplete)         :: 148 of 243
            Pseudo Genes (internal stop)      :: 73 of 243
            Pseudo Genes (multiple problems)  :: 69 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:32:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 115758
            Orig. end                         :: 126082
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAAVIO010000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10324
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10324
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5324](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5324
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(61..74)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.92"
                     /tool="antismash"
     gene            991..2439
                     /locus_tag="HB668_RS27475"
                     /old_locus_tag="HB668_27475"
     CDS             991..2439
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-465). E-value:
                     1.2e-29. Score: 95.4. Matches aSDomain:
                     nrpspksdomains_HB668_RS27475_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="HB668_RS27475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27475"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471599.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.9e-29, bitscore:
                     95.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYFKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLSAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTTLADE
                     LDLMKHGVRWEKGMVVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MKYLLHYMEIKGVDISNKDILITNGFTEGLDIVLSSLAKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNDDGIDTTEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLSPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEKIHARTLLQKCYEKGVTFSPGDVFYSDGGGANTFRLGFS
                     RLKEKEIVSGIQIIGETLKNETWS"
     aSDomain        1426..2385
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HB668_RS27475_Aminotran_1_2.1"
                     /evalue="1.20E-29"
                     /label="HB668_RS27475_Aminotran_1_2.1"
                     /locus_tag="HB668_RS27475"
                     /protein_end="465"
                     /protein_start="145"
                     /score="95.4"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMKYLLHYMEIKGVDISNKDILITNGFT
                     EGLDIVLSSLAKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNDDGIDTTEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLSPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEKIHARTL
                     LQKCYEKGVTFSPGDVFYSDGGGANTFRLGFSRLKEKEI"
     gene            2444..3358
                     /gene="ddlB"
                     /locus_tag="HB668_RS27480"
                     /old_locus_tag="HB668_27480"
     CDS             2444..3358
                     /EC_number="6.3.2.4"
                     /codon_start=1
                     /gene="ddlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="HB668_RS27480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27480"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002038752.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.8e-23,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 6.4e-15, bitscore:
                     48.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1e-09, bitscore: 31.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 7.2e-53,
                     bitscore: 172.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.7e-07, bitscore:
                     24.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRY
                     EGVETPDWIELTKMADLKLDDLDKLGFPLVVKPNSGGSSVGVKIVNDKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSIFDGKHLPIVSIRHAAEFFDYNAKYDDVSTIEEVIELPT
                     EIKERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQSSLLPKSADAAG
                     INYSKLLDMIIETSLRVRKEEGF"
     regulatory      3432..3537
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3664..4353
                     /gene="tenA"
                     /locus_tag="HB668_RS27485"
                     /old_locus_tag="HB668_27485"
     CDS             3664..4353
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="HB668_RS27485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27485"
                     /product="thiaminase II"
                     /protein_id="WP_000144532.1"
                     /transl_table=11
                     /translation="MTFSQTLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITEEEKGHFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYKEWISAY
                     GSDWFRTLVEEQITRLDTIAEQVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     EVKDVIG"
     gene            5001..5324
                     /locus_tag="HB668_RS27490"
                     /old_locus_tag="HB668_27490"
     CDS             5001..5324
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108508.1"
                     /locus_tag="HB668_RS27490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27490"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_097880809.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.2e-38, bitscore:
                     123.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYFEDAARRCGGC
                     GRCGGCGGRCGGGGFRCGGFRCIGCFGCFGCGGCGGCGGCSNCFNGFNGITDTTGTIVT
                     YEY"
     gene            complement(5480..5752)
                     /locus_tag="HB668_RS27495"
                     /old_locus_tag="HB668_27495"
     CDS             complement(5480..5752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088127.1"
                     /locus_tag="HB668_RS27495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27495"
                     /product="hypothetical protein"
                     /protein_id="WP_000088127.1"
                     /transl_table=11
                     /translation="MSRKSDITAKDRKDAGKKALKSAVVKGWINVGKWGFFGDMVAFMD
                     RYYTGYKNYNLKKKRERRADSSSSFWFWCNKSAYKSQRVRQEYKT"
     gene            complement(6190..6537)
                     /locus_tag="HB668_RS27500"
                     /old_locus_tag="HB668_27500"
     CDS             complement(6190..6537)
                     /GO_component="GO:0043592 - exosporium [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343262.1"
                     /locus_tag="HB668_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27500"
                     /product="exosporium leader peptide-containing protein"
                     /protein_id="WP_000343263.1"
                     /transl_table=11
                     /translation="MDEFLSSAALNQNSIGPTLPPMQPFQFPTRPTGNTGPPGIVSVTF
                     NQSHIPATSPTNLVLPAISVGVNQIVKLEGVFRSDYVSLSPLTEFTVSSILALKRGVFT
                     IAVQMELQEDI"
     misc_feature    6661..6677
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
     misc_feature    complement(6661..6677)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.06"
                     /tool="antismash"
     misc_feature    6866..6879
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.16"
                     /tool="antismash"
     misc_feature    7527..7545
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.44"
                     /tool="antismash"
     gene            complement(7645..7944)
                     /locus_tag="HB668_RS27505"
                     /old_locus_tag="HB668_27505"
     CDS             complement(7645..7944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110270.1"
                     /locus_tag="HB668_RS27505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27505"
                     /product="hypothetical protein"
                     /protein_id="WP_079519334.1"
                     /transl_table=11
                     /translation="MSVFINYAITPKVFNPNLTYLPNNRRSFIPTQKESSKNICYLLET
                     YYLGKKIKQLLVNGTTLKASIFVGFHNRSRLATFIDENGDITVVNCERIDAILL"
     gene            complement(8025..8246)
                     /locus_tag="HB668_RS27510"
                     /old_locus_tag="HB668_27510"
     CDS             complement(8025..8246)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807684.1"
                     /locus_tag="HB668_RS27510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27510"
                     /product="hypothetical protein"
                     /protein_id="WP_000807684.1"
                     /transl_table=11
                     /translation="MAEHTKCVCEQLERLQLGTEVDVFLTGTTLEDLIFTNFNPDNFCV
                     TFVDNTTEPGSIIVLDCRDIQALRIEAE"
     gene            8736..9536
                     /locus_tag="HB668_RS27515"
                     /old_locus_tag="HB668_27515"
     CDS             8736..9536
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097507.1"
                     /locus_tag="HB668_RS27515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27515"
                     /product="C1 family peptidase"
                     /protein_id="WP_000097507.1"
                     /transl_table=11
                     /translation="MSSNESNFSFTSNKDIVKYLNVRKDPVDLRDKIFRSYLFSNEADL
                     PTQVDLRPKMSGIVNQWNIGSCTANAIVSGLREYLLLQSGRPFTCLSRLFLYYHEREIE
                     GHIQEDSGANVRDGMDVLKKIGVCPEEDFPYTENFRDKPSPKAESDATLYKIGTYHRIQ
                     DLSGLKAALAHGLVVVLGFAVPKSFMSEEVKKTGIVPVSGTDDVIVEDGQQAGHAVCAA
                     GYDDNKKTVIIRNSWGEEWGDKGYCYFPYELFENGIVQDMWTGR"
     gene            9819..9980
                     /locus_tag="HB668_RS27520"
                     /old_locus_tag="HB668_27525"
     CDS             9819..9980
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866154.1"
                     /locus_tag="HB668_RS27520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_27525"
                     /product="hypothetical protein"
                     /protein_id="WP_000866207.1"
                     /transl_table=11
                     /translation="MKVLKNQLREWKNQSKKKNKKKRKEKFSTREIEDLMGVHRPCYER
                     RRGALRQK"
ORIGIN
        1 tgtattggta gaatcaacag ttacaaatct taaagatgga aatccgggaa aaaaatatat
       61 aaaacaagcg attgagaaac atatggatat tgttgcaatt tcaaaaggtg cactcgttac
      121 taactggaga gaaataaagg cagcagcaaa agaagcgaat gtacgaattc gctatagtgg
      181 cgcaactgct gcagcactac cgacattaga tattggacaa tttagtttgg ctggctgcaa
      241 tattgaaaaa atagaaggaa tattaaacgg gacaactaat tatattctta caaaaatgta
      301 tgaagaagat gttacgtttg aagaagcact gaaagaagcg caaaataaag gaactgctga
      361 gacgaaccca atattagatg taagtggttc ggatagtgcg tgtaaattat tacttttggc
      421 aaacagcttg atgggtgttg agaatatgct tactgatatg catataaaag gaatcgaaaa
      481 tgttacaaaa caacaaattc gacatgctaa ggaacaaaac aattatatta aattaatagc
      541 ttcagcatat aaggatatgg atggtaaagt gaatcttaat gtagaaccgc ttgaaataga
      601 aaaagaccat ccactagcaa aagtaaatgg aactgaaaaa ggaataacat tctttacaga
      661 tacaatggga caagttacta caattggtgg agcttctaat ccacgcggag ccgcagcagc
      721 tgctttaaaa gatgtaatta acttatatcg tagagattta tagaagtaaa actcacacct
      781 tcaaaattcg gatggagaaa aaaagttagg tgggagcctt tttgcccata tatgtccgat
      841 tcgggctaaa aatcggtggg ggatgaataa aattcccacg gattaaagtt tcactttata
      901 agaccgtacc gctgttaata aaaaatacat atgtagtgga ccttttgaga atgaaataaa
      961 tatgcaaaaa taattgcagg gggtaagaaa ttgtttaaag attttaaagt tgtaaaagat
     1021 cgtcccgttt atattcaatt aaaggattat tttaaaaaga tgattatgaa agggcatttg
     1081 ctcggtgatc aaaagatccc atcaacaagg gaactgagtg aattattatc agtgagcaga
     1141 aatacagtac tctctgctta tgcggattta gaacaagaag gactcattta tgcagtaaaa
     1201 ggaaaaggga atttcgttgc gaaggtggat atatctaaca cttcgtctgt tgaaatagat
     1261 tggaaaaata aacttaatac agttaccaca ttagcggatg aattagactt aatgaagcat
     1321 ggagtccgct gggaaaaagg aatggttgtt tttaatagta tagccccgga cgaaaagcta
     1381 tttgatgttg aaaatttcaa aagagctttt cttactcgta tgtccattga aggggatatc
     1441 gtattgaact acggatatgc aaaaggttac cgcccgttaa tgaaatatct acttcattac
     1501 atggaaataa aaggtgtaga tatttcgaac aaagatattt taattacaaa tggatttaca
     1561 gaaggattag atattgtact atcttcctta gcgaaaaaat cagggcgtgt gatttgtgag
     1621 aatccaactc accatgctgc attgaaactt tttcgcttac atggacttga agttcatggg
     1681 atagatatga atgatgatgg tattgatacg actgaagtag aaaaaagttt acgtgaaaag
     1741 gattttgatt ttgcgtattt aattccttct tatcataatc caactggtat tgttacaagt
     1801 tcggaaaaaa gaacggaatt gatgaggtta ttttcgaaat ataaggttcc tattatagag
     1861 gacggattta atgaagaatt acgttattca ggttcacatt tatcgccact attaactttt
     1921 gcaggggctg gtaataatgt gatttatatt agtagctttt cgaaggtact tttccctggt
     1981 ttacgtgtag ggtggattat tgccgataaa gaactgattc attatttaga aagtgttaaa
     2041 agagcaagaa ccattcacac atctacgtta gatcaagctg ttttgtttca atatttacat
     2101 gaaggatatt ttgaaaaata tttaaaaaag gcaagatctg tttacaagaa aaaatatgag
     2161 ttagccgttg gggcttgcaa tcagtatatt ccgtttaaaa ggatgactgg agatggcgga
     2221 cttcatttgt ttatagagtt ggaagagaaa attcacgctc gcacactttt acaaaagtgt
     2281 tatgagaaag gtgtaacgtt ttctcctgga gatgtttttt attctgatgg agggggagcg
     2341 aatacattcc gcttaggttt ttcacgtttg aaggagaaag aaatagttag tgggattcaa
     2401 ataattggtg aaactttaaa aaatgaaact tggagttgag aaaatgagaa ttggcgttat
     2461 tatgggagga gtatcctctg aaaaacaagt atcaattatg actggaaatg aaatgattgc
     2521 acatttagat aaaaataagt atgaaatcgt tccaattacg ttaaatgaaa aaatggattt
     2581 aattgaaaaa gcgaaagaca ttgattttgc attgctagca ttacacggaa aatacggaga
     2641 agatggtacg gttcaaggga cgcttgagag tttaggtatt ccatatagtg gcagcaatat
     2701 gttatctagc ggtatatgta tggataaaaa tatttcgaaa aagattttgc gttatgaagg
     2761 agtagaaaca ccagattgga ttgaacttac aaaaatggcg gatctaaagc ttgatgattt
     2821 agataagtta ggatttccgt tagtggtaaa accaaactct ggcggctcga gtgttggcgt
     2881 aaagatcgtc aatgataaaa atgaattgat ctcaatgctg gaaactgtat tcgaatggga
     2941 ttctgaagta gtaattgaga agtatataaa aggtgacgag attacttgtt caatttttga
     3001 tggtaaacat ttacctatcg tttcaattcg acatgcagct gagttttttg actacaatgc
     3061 aaaatacgat gatgttagta caattgaaga agttattgaa cttccaactg aaattaagga
     3121 acgtgtaaat aaagcgtcat tggcttgtta taaagcatta aaatgtagtg tttatgcaag
     3181 ggttgatatg atggtgaagg acggaattcc atatgtaatg gaggttaata cattaccagg
     3241 gatgacccaa tcaagtttac tgccaaaaag tgcagatgcc gcggggatta attatagtaa
     3301 actattagat atgataattg aaacttcatt aagagtgagg aaagaagaag gtttttaaga
     3361 taattataca attaaaactt ttgatagttc cacgaagtat gctatgataa ttacaatacg
     3421 acaactaaat atttatccac taggggggcc ttttgtaggc tgagatcaaa cataattttg
     3481 agactcttag tacctgatct ggttaatacc agcgtaggga agtgggaaga cgattatatt
     3541 taaagaatac attaatattg tctatttcac tttctttacg cctgtaaaga aagttttttt
     3601 gttttacaga tttaataact gtggataaat acatatattt tatacattat tggaggaatt
     3661 taaatgactt tttcacaaac attacgtaaa gaagtagatt ctatttggga ggcaagtttt
     3721 catcatcctt ttgtaaaaaa acttggtgaa ggtacgttag atttggcaag ttttcgttat
     3781 tacgtacttc aagattcata ttatttaagt cattttgcta gagtacaaac tttaggagct
     3841 gcaaaggctc ttgaattaga aacgacagct cgcatggcac accatgctca aaatacatac
     3901 gaggcagaat tatcattaca tgagaatttc gcaaagaaat taggaattac ggaagaagaa
     3961 aaaggtcatt ttatccctgc cccaactgca tatgcgtata cttcacatat gtaccgtgcg
     4021 gcgtatgaag ggcatttagg ggatattatc gcagcaattt taccttgcta ctggctatat
     4081 tatgaaattg gtgaacgttt aaaagaatgt cagccagaag agccgattta taaagagtgg
     4141 atttctgctt atggatctga ttggttccgt actttagttg aagagcaaat tacacgatta
     4201 gatactatcg ctgaacaagt gacagagtct gatcggaagc gtatgaaaca gcatttcatt
     4261 attagtagtc aatatgaata ttcattttgg gaaatggctt atacattaga aaaatggcca
     4321 gtggatacag aagtaaaaga tgtaattgga taaagagtag aggtattata catatgtatg
     4381 gataatacct ctttatatga tttcaaaaaa ggggtatcgc aaaaaagacg tatcaatcta
     4441 agtgcgtctt tttttcaatt ttcttcaatt agcaatttca tggagagctt gctgaaaaag
     4501 tagaatgaaa agccctaatc agggcttttt ttgtaggaat ttcttaaccg tcattgagtc
     4561 ctgtcactag taggtgtttt gacgctacga cggactaaat cgattattcg gctagtgaag
     4621 tatataaaaa agttaggaat atctgaaggt gctctcagaa agtatatgga agtgttataa
     4681 cgagaggcat ttacagtaca gaaaaatcta agcggcatac gggaatacgt aggatacggc
     4741 atcatggtta ttgagaaatg aattgaactt agtaagcatg aagatatgac gcaagcgttc
     4801 acattttata atttctattt ataaaaatga gcccatagta aaaataggta tatgaacaag
     4861 gtgcaccgtc tagtgatagg tcatacttta taggtgtacg tattgaactt agtttatagc
     4921 gttatttcta cttagattta acgaacagaa atattggaaa gcatcgatga ggttaaagtt
     4981 tataaacgga ggtcatgaaa atgaataagt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattacca gtctggaaat attgtgtatt gggaccagca aaaccaatat ccatactact
     5101 attttgaaga tgctgctcgt cgctgtggcg gttgcggtcg ttgtggcggt tgtggaggtc
     5161 gttgtggtgg tggtggattc cgttgtggtg gattccgttg tattggttgc ttcggttgtt
     5221 ttggttgtgg cggctgtggc ggttgtggtg gttgttctaa ttgttttaat ggttttaatg
     5281 gtatcactga tactactggt actattgtaa catatgaata ttgataagag agaaaaggaa
     5341 tctactttgt aaagcgttat acaattttat aaaagagata aatcaaaaca cacgtagcat
     5401 cttttaattt agagagataa tttatccaac cttttataaa cattgtgact ttatttcaaa
     5461 tcaaaaatcg aatgtctttt tatgttttat actcttgacg cactctttga cttttataag
     5521 cagatttatt gcaccagaac caaaaagaag agctgctatc agcccttctt tctcttttct
     5581 ttttaagatt gtaattctta tagcctgtat aatatctgtc cataaatgca accatgtcac
     5641 caaagaatcc ccatttacct acatttatcc aacctttaac aactgccgat tttaatgctt
     5701 ttttccccgc atctttacga tcttttgctg ttatatctga ttttctgctc attctcatac
     5761 tttatatgaa atatgtgaaa atcagtatga atggtggaag tgagcgcaac tttaaaagct
     5821 atttattggg attaacaaat ttaataatct atttttatta ctattgatta attttgaaac
     5881 gtaacaaaac tacgtaactg tttgtataaa atgcactgct ctagtattca aaatttagaa
     5941 catggaatag ggtgctttgt ttctacaagc tgtttttgtt ttacttcata ttacgattta
     6001 tagaccggga attacacttt aaatgtttat tactgtagca gttatgtttc tttcactaat
     6061 agtgttagtg gatgtaccaa ttcccgtggc attcagagat agcctgtata cataagtacc
     6121 agggggaggc gtatctatcc agactattga aacgttgtgt gttaaatttg ttgaaacatt
     6181 tactggtttt taaatatctt cttgaagctc catctgaacc gcaatagtaa agacgcctct
     6241 ttttagagcg agaattgaag atacagtaaa ttctgtaagg ggacttaggg aaacataatc
     6301 agatctgaaa actccttcta actttactat ttgattaacg cctacactaa tagctggtaa
     6361 tacgaggtta gttgggctag ttgcaggaat atgactttgg ttaaatgtaa ctgaaacaat
     6421 gccaggtggt ccggtgttac cggtcggtct agtagggaat tgaaaaggtt gcataggtgg
     6481 gagtgttggt ccaatcgaat tctggtttaa tgcagcagaa gataaaaact cgtccaatat
     6541 aattcacctc taaaaaattt atattactga tagtaaatgt aggcgttgag caaagtgaca
     6601 tggacaagcg gttgtatgtt aaaaattaaa caaaatagtt attttattaa agaaaaaaca
     6661 tgcacaaaaa ttgcgcatgt ataataaaaa gctatttctt gtctatgtaa atatatgatt
     6721 ggcttataca aaccgtgagt gtgtttgaga aaaatggtta tttattaaca aatataaaga
     6781 gcgcttttaa ggcgctcttt aaccaaaact aatattaaaa aagaataccg caggatattt
     6841 tatgtatgtt cttaataaat gtgcgatttt taaacaaaat ccttatttta tcttaaataa
     6901 aaagagcaca catataagcg tgctctcaaa taagaaaggt agaatgctat gaatggaaag
     6961 cctcgataga ataacatatg cttgtccggt ttaaatgtga gaagttttta ataaaaacgc
     7021 tattttagta aggaacaagt ttaaaaggcc ctgattgaca caggaccttt taaaagggaa
     7081 caacacttga catgttaaaa attatgactg aaaggtattt tcatattacc attagtgggg
     7141 ttttaattcc aataatggtt gttgagaaat aaaaccttta ttttgcacaa caaagcagct
     7201 agctgaagta gctaattgct ttattgtaca attttagaag ttcactggat acagatatat
     7261 gttgtaacaa atagttacat ctatggtata aacaaatacc aaaatattat gcaggaaaga
     7321 aaactaaatt aaaacttcat tttataaaga gagaagcaca aaatattata tgtgtatcca
     7381 gtcaattgag tgcgttttta ctaacaaaga gcatctagca aaagctaact gttcggttct
     7441 ccaaggggga acaaggagaa agtaacttaa tgggttgtct acattattga cggaatctgg
     7501 aggcttattc ggtcaaataa aaaaggtact gtatataagt gcactttgga ccaagttttt
     7561 tatggtaaca gttattggta ttatgtgtaa gaactaacgc gtatcttgaa aataatgatt
     7621 atttatttaa atgtaaattc ataattagag tagaatcgca tcaatacgct cgcagttaac
     7681 tacagtgata tctccgtttt catctataaa tgtagcaagg cgggatctat tatggaatcc
     7741 tacaaatata gaagctttta atgttgtgcc gttaactaac agctgtttta ttttttttcc
     7801 taagtaatat gtttctaaaa gatagcaaat atttttagaa ctctcttttt gggttgggat
     7861 aaaagaacgt cggttatttg gaagatatgt tagattagga ttgaatactt tgggagtaat
     7921 agcatagttg atgaaaactg acatttttcc ggctcctcta ttttataatt tatattgaaa
     7981 tatgctgtgt gaaatagggc ttttttcaaa gccccatctt tactctactc agcttcaatt
     8041 ctaagcgctt gaatatcacg acagtccaaa acaattatag agccaggttc tgttgtgtta
     8101 tccacaaatg ttacacaaaa attatctgga ttaaagttag taaaaataag atcctctagt
     8161 gttgttcctg taaggaaaac atctacttct gtacctaact gtaatctttc taattgttcg
     8221 caaacacatt ttgtatgctc agccatataa aaacacctcc ttttctcaaa ttactattaa
     8281 tatatgtata aattaaatag aacgattgga gtaatagctt agtaatatta attattttgc
     8341 tagttaatat ttttataaaa taatcctttg aaatgaatat cgtccggcta gaaaactaga
     8401 ggacaccaat tcattaaagc agccattaaa gctgttttag gaataggtgt cctttttatt
     8461 ttgaaaaggg agatggggaa atgaatgtgt taagagatca attaagtgaa tagaaaaagt
     8521 aatcgaaaca aataaggaag aaaaaaaata tagagttcaa gtggttgtga gataaattga
     8581 aaatagaaat tgataattaa aaagataaat aattaaaaat ttggatgtag tgaatgcaaa
     8641 tgaaattaaa attaaaaagg tataatttta atttttaagt taagtataga aaagaaatga
     8701 atcatctaga tataaagaaa ggatggattt ttgcaatgag ctcaaatgaa tcaaatttca
     8761 gttttaccag taacaaagat attgtaaagt accttaatgt aagaaaagac cctgttgatt
     8821 taagagacaa aatatttcgg agttacttgt ttagtaatga ggcggatttg ccaacgcaag
     8881 tcgatcttcg tcctaaaatg tcaggtatag taaaccaatg gaatataggt tcttgtacgg
     8941 cgaatgcaat tgtttcaggg ttaagagaat acctattatt acaatcaggt cgtccattta
     9001 cttgcttatc tcgtttgttt ttatattatc atgagcgaga aatagaagga catattcagg
     9061 aagatagcgg agcgaatgtt cgtgatggta tggatgttct taaaaaaatt ggggtatgtc
     9121 ctgaagagga ttttccttat actgagaatt ttagagataa gccatcacca aaggctgaat
     9181 cagatgcaac gctttataaa attggaacct accatcgtat tcaagattta tcgggattaa
     9241 aggctgcact tgcccatgga ttagttgttg tacttggatt tgctgtgcca aaatctttta
     9301 tgtcagaaga agttaaaaag actggtatag ttccggtatc aggaacagat gatgtaattg
     9361 ttgaagatgg tcaacaggca ggacatgctg tttgtgctgc tggatatgat gataataaga
     9421 aaactgttat tattcgaaac agctggggag aagagtgggg agataaagga tattgttatt
     9481 tcccatatga attatttgaa aatggaattg tacaagatat gtggacaggt cgttaattag
     9541 ataaagaagt ggaagtatgg aaacaataaa acacgttata catgttacgg gtagtataaa
     9601 agagcttgaa aaagatgtgg aaataaaggg agtcaggttt gttacctaca acggaaaaag
     9661 atatgagtaa tatttttata caagctggta ttttaagtga acgaaaaaca aatatagtcc
     9721 ggctagaaaa ctagaggaca ccaattcatt aaagcagcaa ttcaagctgc tttaggaata
     9781 ggtgtccttt tttttatttt ggaaagggag atggggaaat gaaggtgtta aagaatcagc
     9841 tacgtgaatg gaaaaaccaa tcgaaaaaga aaaataagaa aaaacgaaaa gagaaattta
     9901 gtactcgtga aattgaggat ttgatggggg tgcatagacc ttgttatgaa cgcagacgtg
     9961 gagcattaag acaaaagtaa tttaaaaata aaaaggagtg gtcttacatg actaaacaat
    10021 tatctttctt accaaaaatt gatagaacag cgacacaaga ggaattagaa ggtgtgttgg
    10081 aaagtgtacg tatacataga caatttggga tgatgcgtaa agaaatgaaa gtcactcctt
    10141 cttatgaaat acgtgagcac ggtcctacac atacagttgg taagctgtta gaagatgttg
    10201 ctatagcaaa tattcaacaa agcaaacgag aagagtggct tgaaagaatg tcagtacgta
    10261 ttgatcagtt tctaaatcga ttagggaacg gacgtgcagg aagcattcaa agagatatta
    10321 ttta
//
