LOCUS       NZ_JAAVIO010000006     10267 bp    DNA     linear   CON 03-AUG-2024
DEFINITION  Bacillus toyonensis strain M5 contig6, whole genome shotgun
            sequence.
ACCESSION   NZ_JAAVIO010000006
VERSION     NZ_JAAVIO010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 283856)
  AUTHORS   Huang,Y., Palmer,J.S., Ding,Y. and Yu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) School of Food and Advanced Technology,
            Massey University, Private Bag 11222, Palmerston North,
            Manawatu-Wanganui 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012395365.1-RS_2024_08_03
            Annotation Date                   :: 08/03/2024 02:52:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,219
            CDSs (total)                      :: 6,078
            Genes (coding)                    :: 5,835
            CDSs (with protein)               :: 5,835
            Genes (RNA)                       :: 141
            rRNAs                             :: 17, 6, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 7, 6, 13 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 103 of 243
            Pseudo Genes (incomplete)         :: 148 of 243
            Pseudo Genes (internal stop)      :: 73 of 243
            Pseudo Genes (multiple problems)  :: 69 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:32:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 206319
            Orig. end                         :: 216586
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAAVIO010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5267)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    878..891
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.47"
                     /tool="antismash"
     misc_feature    complement(1012..1027)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.2"
                     /tool="antismash"
     gene            complement(1074..1517)
                     /locus_tag="HB668_RS20670"
                     /old_locus_tag="HB668_20670"
     CDS             complement(1074..1517)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757631.1"
                     /locus_tag="HB668_RS20670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20670"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757645.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGKNGSYYTLPGGSVKFGETL
                     EEAVIREVKEETGLHITVKGICSISEAFFEERGHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEQLLDFLQRKETVPYFFNGTIVHQSL"
     gene            complement(1672..2088)
                     /locus_tag="HB668_RS20675"
                     /old_locus_tag="HB668_20675"
     CDS             complement(1672..2088)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="HB668_RS20675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20675"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706728.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHLSNEEKTPSEKVSFIVKNE
                     KEEIVGGITAITFWHHVHVDFLWVSEECRHEGYGSKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGFIVIGVSEDHPKGHNHYYLEKRL"
     gene            complement(2222..3430)
                     /locus_tag="HB668_RS20680"
                     /old_locus_tag="HB668_20680"
     CDS             complement(2222..3430)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="HB668_RS20680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20680"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000815141.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQRKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDSLGVS
                     QAFLYWGIIVLLLVLIGSLFLREAIVSNTVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAVFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGVSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCAISFIISILIRPPNVEKKKEIKQLHR
                     KIA"
     gene            complement(3711..3854)
                     /locus_tag="HB668_RS20685"
                     /old_locus_tag="HB668_20685"
     CDS             complement(3711..3854)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="HB668_RS20685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20685"
                     /product="hypothetical protein"
                     /protein_id="WP_000653682.1"
                     /transl_table=11
                     /translation="MKDKVSKDKKQNIKDVNTEQNAIFSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            complement(3886..4902)
                     /locus_tag="HB668_RS20690"
                     /old_locus_tag="HB668_20690"
     CDS             complement(3886..4902)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="HB668_RS20690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20690"
                     /product="serine hydrolase"
                     /protein_id="WP_000288924.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTASSQFNVASARKSYLGFMVAYALYDGKINSIDDEAIKYFKDFDPALLDKTTI
                     RHLVTHSHGLGETDDGTIFREFEAGQDWAYRDINVRMMTHLIYRLYNKSFPELLKERVF
                     TPANFQETGWRIQQNENLVNVVNNPNEDAISEVGTVDDGTEKNLFVSAREFAYWGNLHL
                     NQGMINGKQVVPKEVIKIATSLQSPAYKNNELPQNGLFWFVQNEPKQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFTSCNRA"
     gene            complement(5001..5267)
                     /locus_tag="HB668_RS20695"
                     /old_locus_tag="HB668_20695"
     CDS             complement(5001..5267)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="HB668_RS20695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20695"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071376.1"
                     /sec_met_domain="TIGR03601 (E-value: 6.4e-49, bitscore:
                     158.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCVGCAGCFSCFNCWNWWII"
     gene            complement(5465..5855)
                     /locus_tag="HB668_RS20700"
                     /old_locus_tag="HB668_20700"
                     /pseudo=""
     CDS             complement(5465..5855)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="HB668_RS20700"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20700"
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIAEYIGHHYFLPGGHVEIGESAENALIRELREGA"
     gene            complement(5936..6394)
                     /locus_tag="HB668_RS20705"
                     /old_locus_tag="HB668_20705"
     CDS             complement(5936..6394)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="HB668_RS20705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20705"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098657912.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALQLQVKEDQRKFVPAVAVSLAKVYIKPDG
                     DNVEYIPFAIYVSNLMVGFIMHAVVRETSNMYWINGFIIDQKQQGNGYGKSAVQESIYL
                     IKNKFKACKEIRLTVHKENISAKKLYEHYGFQPLGQDYDGEQVYRLFV"
     gene            complement(6471..7394)
                     /locus_tag="HB668_RS20710"
                     /old_locus_tag="HB668_20710"
     CDS             complement(6471..7394)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="HB668_RS20710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20710"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_000416770.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEISTYYPEIHGLEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLILNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFQQAKNLFIFPLNVTNKAVLTPNVFNYIQANSKNPFHTIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFAHRTVNVDTYGDTKGQTFADFRPS
                     SISKGARIALEIDEQKFIQDFIKIML"
     gene            complement(7558..8919)
                     /locus_tag="HB668_RS20715"
                     /old_locus_tag="HB668_20715"
     CDS             complement(7558..8919)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080598.1"
                     /locus_tag="HB668_RS20715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20715"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_098631434.1"
                     /transl_table=11
                     /translation="MNRKRNIFICLSIIGIGLASVSNSSFAANVTDSSVQHSNPVMNQQ
                     VAVATEMKPFPQQVNYADVIKPNHVTQESLNASVISYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMVITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADNKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGSVNYLKEAQNMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFNN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESKYTNSYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSNNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKETQNLINDEMYEGYDK"
     gene            complement(9058..9972)
                     /locus_tag="HB668_RS20720"
                     /old_locus_tag="HB668_20720"
     CDS             complement(9058..9972)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="HB668_RS20720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_20720"
                     /product="DMT family transporter"
                     /protein_id="WP_097896126.1"
                     /transl_table=11
                     /translation="MLNKKWDLQIICAHAFTILIWGTAFPAIRMGLESYTPEHLTLLRL
                     LIASFILLVFSFIYKLRLPDLKDIPAIFIFGALGFTIYHIALNYGEKTVNAGSASLIIS
                     VTPIVTAILASVFLNEKMKLNGWIGGVISFIGIALISFSQGGSIQFNSGGLFILLAAVS
                     ESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGIYTETLAAPLEVNVSVIYLGL
                     FPTVLPYLALAYIISRAGASEATSSLYLTPVTACFIAWVWLGEVPTFISVIGGGITILG
                     ILIAHIRVVRKEKHRNLVNNKSI"
ORIGIN
        1 cttaaagtaa ccatttcttc tgttattgcc cttaaagcat ctaccataaa tcgtccgtcg
       61 aaagatatat ttaattgttt ttcaccttga atcgcatcta cttgttgtgt ttcagctatt
      121 ttaccaatct gagaagcgtt agaagaaatt ttaattgtgg attcattgat gatttctaag
      181 ttgacgttgt tatttgccca ttcacttgct aataagctcg atcgatctac accttgtaaa
      241 atcttttttc tttctacgtt aataaccgtt tgaaattcat ttggaatgag attagatata
      301 ttaggatatt tcccttcaat gagtcttgaa tataacgtag ttgttccgaa tgtaaaaata
      361 acgtgactgt ctgaaaggta tatgtatacg aattccgtat tactgttcat taattttaga
      421 agttcactaa tagttgcgct tggtacgata caatttgctt tcgcatctga agaaattggc
      481 gtttcacgta tagctagtct atgtgagtca gtagcagcgc aaactaattt attgtgatct
      541 aactctatat gtactccggt gagaacaggt ctggattcat ttttagctac tgcaaaaaca
      601 gtttgtttaa acacttcaat caattgttct gtttctactt gtatttctgc atgatcccct
      661 ataagtggta cattcggaaa ctcatttgct ggaagtccat ttaagtttaa tgtgatttct
      721 tctgattgta tcgtaatgat ttgctcattt ttacttttta ttactatatc acttggcatt
      781 ttctttataa tttcaatgaa atattttgca ggtacaacaa tagttcctgc ttgtaatata
      841 gttgcaatct gttcgtcttc gattgaatta ggtataaatt ttttaataaa aacattcgag
      901 ttacttgcga ttaaagtaat tccagattga tcggctgtta ttttgatacc agataatata
      961 ggaattaaaa cttttgtaga tatagcttta cttacttctg aaagtgcttg agtgaaatgt
     1021 ttgtgactaa cgataaactc cattttttcc ccgccttaaa tgaaataatt tatttataaa
     1081 gattgatgaa cgattgttcc gttaaaaaag taaggtactg tttctttcct ttgtaaaaaa
     1141 tctagtaact gttcaggtat acgtaaatac ggtgctgcta tatgtaattc catccaagta
     1201 atctcttcga tttcttttgg acgggatata tatgtctctc cgccgattat ttcacctaaa
     1261 aaattaaaga aaatggcatg atggcctctc tcctcgaaaa aggcttcgct tatggaacaa
     1321 atccccttta cagttatatg tagccctgtt tcttctttta cttctcgaat gactgcctct
     1381 tctaacgttt ccccaaattt aactgagcca cctggtaatg tgtaataaga cccatttttt
     1441 cctttgtttt ttaccattaa gattttttcg ttcgttttat cgtacaaaag cgcgtatgta
     1501 acattaacct ttttcatatt taccccttta ttaataagaa ttttctgcct ctatttcatt
     1561 ttaccagata catacaaatt aaaaagaacg tattttgata tagatgattc aaaatacgtt
     1621 ctttttaatt tattgaattt tatatattga gattaattaa ttctatacac tttataacct
     1681 tttctctaaa taataatgat tatgtccttt tggatgatct tcactcactc caatgactat
     1741 aaatccatgt tttttataaa aatccggagc ttgaaagcta aacgtatcta agttgattag
     1801 cctacatcct ttttcaattg caaattcttc gataagtttt attaatttac ttccgtatcc
     1861 ttcgtgtctg cattcttcag aaacccatag gaaatcaaca tgtacatggt gccaaaatgt
     1921 tatagctgtt atcccaccta ctatttcctc tttttcattt ttcactataa aactaacctt
     1981 ttccgaaggt gttttctctt catttgaaag atgtttttga ttatattcaa ttactttctc
     2041 tcgtatgtaa tcactatctt cttgaaccca ttgctttact atcttcattt tacatatcac
     2101 actcctttta ttttatctat tccatttcat aattgtattg tcctgtcttt actaatcaat
     2161 gaaaggagca tagttaacgt tgtaatgaaa aagagcccta cgataaggcc tctttttaaa
     2221 actaagctat ttttcgatgt aactgcttga tttccttttt cttctcgaca tttggcggac
     2281 gaattaatat agaaataatg aaagaaatcg cacataaaac acctattgta ataaaggttg
     2341 gctgaaatcc accaagtaat gctccaatga atgaacctgc aagtgcacca aatccgaaac
     2401 cttgatatac aatcccgtaa tttgtactat gattttttaa accgaagaaa tctccgacaa
     2461 tggctgggaa tattgtaata ttaccgccga agcaaaaggc gacacttgct acacaagcaa
     2521 aataaatccc gtaatttaat ggaatgaaac taagagtaaa gacggatact ccaataataa
     2581 taaatgttgc agagacaatt ttcattctac caattttatc tgataacgtt ccaagaatga
     2641 ttctacctac tgtattaaag actgcaatca ttgcgacggc gttagcggcg gttgctgcgc
     2701 taagtccaac gagttgtacg ccgatatctt ttaccatacc gattaagtac agaccaccca
     2761 tacacgatgt gaataacatg aaaaacaata gatatacttg tttcgttctc atcatttcac
     2821 gtggcgtata atcattgtgt aatgtttctg ttaccgtatt acttacaatc gcttcacgta
     2881 agaagagcga tccgattaat actaaaagta agacgataat gccccaatat aaaaatgctt
     2941 gtgatacacc taggctatcg ataagatttc cgtttatata tctaaaaatt aaactgccca
     3001 ttccatatgc tgaaacagat attccagaaa taagtccttt tcgatttgga aaccatttaa
     3061 ttaaattaga tagtgacgta atataagctg ttccatccgc atacccaaca acaacaccag
     3121 ctaataaata tagtaatggt aaggaagaaa cttgtgaact aagtattaaa ccgagtccta
     3181 gaacaattcc tgcagtagcg ataagtttac gaagtcctaa ttttcgctgc aactttgctg
     3241 caaacaaggt tgaaaatgat aaagaaaagc ttgttatgga gaaagttatc gcaactgaat
     3301 tgagattcca tccgaactta cttacaaggg gttgattaaa taaactccat gtataaattg
     3361 ttccaaggcc aatttgaaca atgattgtac ctagaacaat taatagcgga tttatagaag
     3421 tttgtttcat actaactgtc cccttctttt atcttgacat gaattttcac agttagtata
     3481 tccatcataa attgcaagat actgtacgaa aaatggaaat taagtttatg tacacaaaaa
     3541 gacacttcat catgaagtgt cttttcgcag gttatatcag atacatttaa agaaagcttt
     3601 gttaagataa gccattttca agtgcttatg tatatatgtt atcaaatatt ttcttacatg
     3661 tgatgggact taagacgtat tttttattcg tcatatagtc ggatgagaaa ttaatattcg
     3721 atttcatcaa aatctttcgg ctgcggtaca gtttgcatgt tagctgcatc tttcggatcg
     3781 ctaaaaatcg cattttgctc tgtatttaca tcttttatat tttgcttttt atctttgctc
     3841 actttatctt tcaaattctt tcactccttt ttgttatagg agtaattatg ccctattaca
     3901 gctagtaaat gtatcggcga ctaaattgct aaattcacgt aaataatata agtaattatc
     3961 accgccgtag ttatatcttt tattgtacat tttcgcaaca actacattat actcaggtat
     4021 tactaaaatg gttggtccag taattcctaa tatttgatac gaaccttttg gaacacgttc
     4081 accaagttca cttaattgtt ttggttcatt ttgaacgaac caaaacaacc cgttttgtgg
     4141 tagttcatta tttttataag ctggactctg caagctagta gcaattttta taacttcttt
     4201 tggaacaact tgtttaccat ttatcatgcc ttgatttaaa tgaaggttgc cccaataagc
     4261 aaactctcta gctgagacaa acaggttttt ttcggtaccg tcatctactg taccgacctc
     4321 actgatcgcg tcttcatttg gattattaac aacgttaact aaattttcat tttgctgtat
     4381 tctccatcct gtttcttgga aattagcagg agtaaataca cgctccttta acaattcagg
     4441 aaaactttta ttataaagcc gataaatgag atgcgtcatc attcttacat taatatctct
     4501 atatgcccaa tcttgtccag cttcaaattc acggaaaatt gtcccgtcat ccgtttctcc
     4561 tagaccgtgt gaatgagtta ctaaatgcct aatcgttgtt ttatcaagta atgcaggatc
     4621 gaagtcttta aaatacttta tcgcttcatc atcaatagag tttattttcc catcataaag
     4681 cgcatacgct accatgaatc ctaaataact ttttctcgca gaagcgacgt tgaactgtga
     4741 agatgctgtt acttttttac tagtagatgt atttgaatga tatccactat aatgctctag
     4801 tacaattttg ttatccttta taatacatag tgctgtagca gaactatgat ttttttcttt
     4861 tatattctct acccaagaaa tcaacttttc gtatgtatac aaataaatcc cccttaatac
     4921 tcataaaaaa tcacaacatg aaagaaaagt attactataa acacctttct ttcatgtgaa
     4981 atattattga ttttctatac ttaaatgatc caccagttcc agcaattaaa gcaactgaaa
     5041 catcctgcgc aacctacaca tccgccgcaa cgtcctgcac aaccaccgca tctgccgcca
     5101 catccaccac agcgtccacc acaacctccg catccaccgc aacgacgtgc atcgtcttga
     5161 atataatagt atggatattg attttgttgt tgatcccagt aaactacatt accagattga
     5221 taatcattaa ggtttaatga ttgtagttct tgttgaaact gattcattac gaacccctcc
     5281 atttataagt aattccgccc aaatgtaaaa agtttatata tttttgtaaa ccaaatagaa
     5341 acaaagctac agactaaata cgtacatcaa caaaatatga acttacacta ggtgatgtac
     5401 cttgtttttt agcctatttt ctttatatcc tttcgaagga cgtatttact atacatagag
     5461 tgatttaaaa attacttatc caacaattta gtagttcatc atttaatttt ctttctagta
     5521 attctttaac ggagggcatt ggcatgattt tataagtttt taaagattct ttactataat
     5581 ctatccaatg aaatgctaaa tgagattctc tagaatttgg tggtaaatca atttgtaact
     5641 cttttgaaga tacttcaaaa atgtggttga tttcatgatg aagtatttct ttatcttgcc
     5701 attggttttc tataactcct aaaaatcgtt ttatactgca gtttacttca agctccttct
     5761 cttagttctc ttattaatgc attctctgct gattccccaa tttcaacatg gccgccaggt
     5821 aaaaaatagt gatgcccaat atattcagca atcaataatt tttcatcttt ctagtcattc
     5881 actattacct tgattaaagt agttattttc atttcacgct aatacttaat aaaagttaaa
     5941 caaataaacg atatacttgc tcaccgtcat aatcttgccc taatggttga aaaccgtaat
     6001 gttcatatag tttctttgca gagatattct ctttatgtac tgttaatcta atttctttac
     6061 acgccttaaa tttatttttt attaaataaa tgctttcttg tactgctgat tttccataac
     6121 cattgccttg ctgcttttga tcaataataa acccgtttat ccaatacata tttgatgtct
     6181 ctcttacaac cgcatgcata ataaaaccaa ccataagatt actaacgtat atagcaaatg
     6241 gtatgtactc tacattgtca ccatctggct ttatatatac tttagcaagt gaaactgcta
     6301 cggcggggac aaatttcctt tgatcttctt taacttgtaa ctgtaatgcc tcttcccaat
     6361 tctctctcgt aacaaccttt aactggacat tcaatattgt aagctccttt ataaagagga
     6421 tatattttta tattcaacgt agtgaaataa ttcccttttt cgacaacagc ttataacatg
     6481 atttttataa aatcttgaat aaatttttgt tcatctattt ctaatgctat acgggcccct
     6541 ttagatattg agctagggcg aaaatcagca aatgtttgtc ctttcgtatc tccgtatgta
     6601 tctacattca ctgtacgatg cgcaaattca aaaaaacttg ggttaactaa tccacttata
     6661 gcaaccacat catgtagcaa aggcccttcg atagatggat taagtttttg gtatgcagat
     6721 aaatagtaat catacatcgg tttcattatc gtatgaaatg gattctttga attagcttgg
     6781 atataattaa atacatttgg agttaacaca gctttatttg ttacatttaa agggaatatg
     6841 aataaattct ttgcttgctg aaaaacaatt ttacttgcta taggatctcc atacacattt
     6901 gcctctgcta atggagtaac gtttcctact tctaaaaaga caccacccat gaaatatatt
     6961 ccttttacat ttagtattaa gtcattccat aaattaaaag caatcgctaa tgaagtagat
     7021 cttcctacat caacaattgt taaatctttt ccatacttta caagaattgc agcaatacta
     7081 ccaaaattat atatcggaat agataatacg ctttctggaa catgtatggg ccctaatcct
     7141 tctaatccat gaatttcagg atagtacgtt gaaatttctt cagtaagtgg ttttgttgcg
     7201 ccgctaatga caggaatgtc ttgcctattt gctaactgta aaatatacgc cgcattatgt
     7261 gcagcatgta tatgctcaac atttccatat ccagttacaa taccgacaat ttcaatttcg
     7321 ggatgcagta aaccatacat aatggcaaat gagtcatcaa ttcctggatc tccaaaaaat
     7381 aatacttttt ccaagaaata ctctccttcc ataaaaaaat aaagcaaaca atagttatgt
     7441 atatgaaaaa tgcaagtata tctatgcaaa ttttagaatg gataatgtgt tacgggatat
     7501 taaaaaggaa ctagcgcatg ctaattcctt ctcccagcta accattatca aattctatta
     7561 tttatcatat ccttcataca tttcatcatt tattagattt tgtgtttctt tatcatcagg
     7621 tacaggcttc caccaatttc ccgtaataaa tagcatcgtt aataaattat aactatcact
     7681 aaaatagctt tctcgcttat tcttcatcca atcccaacca ctatttaccc acttttgatt
     7741 attattactc gttatacttg cagcaataaa tggtgaaacg aatacagcag ttgaataact
     7801 accaatattt gatccgttta attgatagcc atccacaatt ttagaaggat ttccattcgt
     7861 cttattttga atccatgaag agactttatc agagatgact ttacttcttt tctcgccgta
     7921 catcgcataa tccattacaa ttcttaatgg tacccgacta gcattgtaat aatatgaatt
     7981 tgtatatttt gactcatcta agaaatcttt aggtgcgggt tgtggtggat tttttacaac
     8041 aaaatctgaa ataagccctg tatttggaga gtacttatta ttaaattgcg tatatacatc
     8101 gtacaaatta ttaataacag taagccaagt tttatcacct gtaaattcat aaaatgctct
     8161 aaggtgtgac atcatccaat cagatggcct tgtatcaagt gaacttttag aatcccaatc
     8221 tcctaaattt agtcggttat tattcgtaac attactagct ttaatacctt ttgtaatcat
     8281 gttttgtgct tctttcaaat aattaactga tccatttgat ccccactgct tatgagcaag
     8341 gagaagtgaa tatgcaatat ctaaatctcc atcagtagca gaatcaaaat gtccttgtgc
     8401 ttttttatta tctgctacaa cccaccccat taaattagga ttttgagagc ttttaaaagt
     8461 tcttgctgtt ttaaataaac catcatagat tttttgggca ttcgaatcat aaccagccat
     8521 taatactgta attaccatcc catacccttg tccctctgaa gttccaagtg gcttaaaccc
     8581 atcggcatca cccgtaatct ctccttttac ataataacca ccaggtaaag aagataaatc
     8641 atttttcaaa tatttctttt tccaattatc gtagtaactt attacagaag catttaagct
     8701 ttcctgtgta acatgattcg gttttataac atctgcataa ttaacttgct gaggaaatgg
     8761 tttcatttct gttgcaacag ctacttgttg attcatgaca gggttagaat gttgtactga
     8821 actatccgtt acatttgctg caaaactaga attagaaaca ctagctaatc cgatacctat
     8881 aatagaaagg catataaaaa tatttctttt tctattcatt atgttgtcat ctccttttaa
     8941 aatgtttact atataaaatg taacatacaa tatttaactg aaatacaaaa tattatattt
     9001 atcaacataa gaaagaaact atatcaaaaa gtatcataaa atcattatct aattaagtta
     9061 tatcgattta ttatttacta gattcctatg tttttctttt cttactactc gtatatgagc
     9121 aatcaatatc ccaagtatag taatcccacc tccaattaca gaaataaaag tcggtacttc
     9181 acctaaccat acccaagcta taaagcacgc agtgactggc gttaaatata gagaacttgt
     9241 tgcctcggaa gcaccagcac gagatataat atacgctaag gcaagatacg ggagtactgt
     9301 tggaaataag cctaaatata taacactcac attcacttca agaggagctg ctagtgtttc
     9361 tgtataaatt cccggtaaga aaataagcat acatacagtg ctagataaaa ttgtatatat
     9421 ggtaaatggc aaaaagccgt actttttcaa gtaagaagtt tggaaaacga aaaataggct
     9481 ttctgaaacg gctgctaata atataaataa tcctccacta ttaaattgaa tagaacctcc
     9541 ttgactaaat gatatgagag caattccaat aaaactaatt acaccaccga tccagccatt
     9601 taatttcatt ttttcattca aaaaaacaga agcaagaatt gccgtcacta ttggagtgac
     9661 tgaaattatt aaacttgcag atccagcatt tactgttttt tcgccgtagt ttaatgcaat
     9721 gtgataaata gtaaacccta aagcaccgaa tataaaaatc gctggaatgt ctttcaaatc
     9781 aggtaatcgc agtttgtata taaaggaaaa cacaagaagt atgaatgaag cgattaacaa
     9841 gcgtagtaaa gtaagatgtt caggtgtgta tgattcaagc cccatacgaa ttgccggaaa
     9901 tgcagttccc catattagaa tcgtaaaagc gtgagcacat atgatttgta aatcccattt
     9961 tttattaagc atgttttctc ttcctctcca ataaaccaac tattttcaaa ttgccaatta
    10021 tttcttttca tcatatttaa tagttgatac aatgtcttca accagttatt tatcgaaaaa
    10081 accaaccact tttcattttt aaataaacga gggagttttt tatatgacaa tcgataaaaa
    10141 ggtgcccaaa tatcggcaga taatagactg tatgaaagaa aaaattgaaa acggagaatg
    10201 gccaatcgga agtaagattc ctagccagcg gcagttagca aaattatttc atgtaaatcg
    10261 gagtact
//
