LOCUS       NZ_JAAVIO010000005     10288 bp    DNA     linear   CON 03-AUG-2024
DEFINITION  Bacillus toyonensis strain M5 contig5, whole genome shotgun
            sequence.
ACCESSION   NZ_JAAVIO010000005
VERSION     NZ_JAAVIO010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 293069)
  AUTHORS   Huang,Y., Palmer,J.S., Ding,Y. and Yu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) School of Food and Advanced Technology,
            Massey University, Private Bag 11222, Palmerston North,
            Manawatu-Wanganui 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012395365.1-RS_2024_08_03
            Annotation Date                   :: 08/03/2024 02:52:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,219
            CDSs (total)                      :: 6,078
            Genes (coding)                    :: 5,835
            CDSs (with protein)               :: 5,835
            Genes (RNA)                       :: 141
            rRNAs                             :: 17, 6, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 7, 6, 13 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 103 of 243
            Pseudo Genes (incomplete)         :: 148 of 243
            Pseudo Genes (internal stop)      :: 73 of 243
            Pseudo Genes (multiple problems)  :: 69 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:32:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 187713
            Orig. end                         :: 198001
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAAVIO010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            480..635
                     /locus_tag="HB668_RS19150"
                     /old_locus_tag="HB668_19150"
     CDS             480..635
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056873.1"
                     /locus_tag="HB668_RS19150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19150"
                     /product="hypothetical protein"
                     /protein_id="WP_000056873.1"
                     /transl_table=11
                     /translation="MSLPTQKKMLQRVKKEATSYCNVDPLAFAIGIRNGFLMVMPFWLV
                     VLYLVI"
     gene            666..788
                     /locus_tag="HB668_RS31325"
     CDS             666..788
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562068.1"
                     /locus_tag="HB668_RS31325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000562068.1"
                     /transl_table=11
                     /translation="MIASNGIIDIHSKKQAQEFTMEMTKQMLHETVGFKWGRNA"
     gene            1041..1322
                     /locus_tag="HB668_RS19155"
                     /old_locus_tag="HB668_19155"
     CDS             1041..1322
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053163.1"
                     /locus_tag="HB668_RS19155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19155"
                     /product="hypothetical protein"
                     /protein_id="WP_000053166.1"
                     /transl_table=11
                     /translation="MSLITPHYLLVGGCSKDKVLQAHKKAKEIFNQNNEINKLISPLKS
                     DSFFILYDGSHHRWKNEDEYMKAKTAYIQYLVESDIQFVEMATQELIS"
     gene            1889..2584
                     /locus_tag="HB668_RS19160"
                     /old_locus_tag="HB668_19160"
     CDS             1889..2584
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207761.1"
                     /locus_tag="HB668_RS19160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19160"
                     /product="response regulator transcription factor"
                     /protein_id="WP_000207755.1"
                     /transl_table=11
                     /translation="MTTILVLEDDITIRSFITLNMKRAGFNVLETDTGEKALELLEEHN
                     VDIVILDVMLPGIDGFRVCKSIRKNNEDIGIIILTGLVQEKDQVHGLTIGADDYIKKPF
                     SIIELVARVQSLLRRIQKNKKDLELIHSGPFQLNLLLGELYRNEIVIDLTPTEHIILRY
                     LMENSMKPISRNELLNKIWGINCVGNTKVIDVNINRLRQKIETSPSNPEFLLTVRGKGY
                     RWNENKHCN"
     gene            2848..3159
                     /locus_tag="HB668_RS19165"
                     /old_locus_tag="HB668_19165"
     CDS             2848..3159
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637520.1"
                     /locus_tag="HB668_RS19165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19165"
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="WP_000637486.1"
                     /transl_table=11
                     /translation="MIVTTTNTIQGKEIIEYIDIVNGEAIMGANIVRDIFASVRDVVGG
                     RSGAYESKLKEARDIAMEEMKQLATQKGANAIVGIDVDYEVVRDGMLMVAVSGTAVRV"
     misc_feature    3304..3318
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
     gene            3680..3967
                     /locus_tag="HB668_RS19170"
                     /old_locus_tag="HB668_19170"
     CDS             3680..3967
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107136.1"
                     /locus_tag="HB668_RS19170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19170"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000935902.1"
                     /transl_table=11
                     /translation="MLNYEKDAEVFKALAHPVRIEILKELIIRGACNVTQIVGILGIPQ
                     STVSQHLAKMKSQKLIRNDRKGLEVYYSAQNKTINSIVEILLDYKSTNCK"
     gene            <4010..>4118
                     /locus_tag="HB668_RS31510"
                     /pseudo=""
     CDS             4010..>4118
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259872.1"
                     /locus_tag="HB668_RS31510"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MS"
     gene            complement(4270..4404)
                     /locus_tag="HB668_RS31330"
     CDS             complement(4270..4404)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653179.1"
                     /locus_tag="HB668_RS31330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653179.1"
                     /transl_table=11
                     /translation="MKDIYELLQEIHINLTEFEERKCSEVEKEKVKGFVKELIKKKED"
     misc_feature    complement(4737..4750)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            5001..5288
                     /locus_tag="HB668_RS19175"
                     /old_locus_tag="HB668_19175"
     CDS             5001..5288
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768886.1"
                     /locus_tag="HB668_RS19175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19175"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000768886.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.2e-17,
                     bitscore: 56.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKKTVSSLVLSAAILGSGATAAFAATQNVGGGTWNYGTGGGSV
                     WSNYYHGSKVHKSSVIGQYYYSSPWTNPGYTSYASAPDTAGVDESYWSVK"
     gene            5466..7685
                     /locus_tag="HB668_RS19180"
                     /old_locus_tag="HB668_19180"
     CDS             5466..7685
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154969.1"
                     /locus_tag="HB668_RS19180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19180"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_168387781.1"
                     /transl_table=11
                     /translation="MKKILITLVLVAYAVSSFVFTKHFEKREMVNINFSDRNGIQVVEL
                     NKDERPEDKLRKIEELSKKEKINIYKLSYKPIKTSKKQKVIIYAAIGDETQFKQKYPLE
                     WGKIFNQDHSVASYLSSSDTKDKEQVGKINIFSPELMVEIKPIEAAKDENLRGAYFVDS
                     NNPNIVQKVKEKLNTEIGFKTEESERAGAMNILENQGVDKYFIIVLTIVLLLIFLSFLY
                     YILLNYKSLAIRKLFGYTDRELIFKHLLKESIQIHTLSLMVVILGQVIYLFFYNGMSKF
                     LDFGSTWIVWQLIFSGISILVSLLPSLMIYHIKILELLKNKKPLKLVQFLNYSSKFIFS
                     IFLIIFSFNLINYYQDLSVQNSNQEQWDTTKNYSFYEYQSNIIGDRTNWEYETGIKSKK
                     LFGITNQKGALLIKPSDGITYKKQLEYNNSLNNTIGDVKEYDPHGGNTLQVNSNYLHDN
                     PVYDLNGKKINIDADYGDYLVILVPNKYRSNEADILKMYKEWYQFTRFIDEDKHKESIG
                     EKIEPHKQVEIKLTYIQDNQKHFLYNPMLEVKNQNYSNGAILIVVNSENMGGDSYLSYL
                     TGRYFFPKVDNLSQPYKELKEDIKIAGLQDIILTTPSLYSIVDDYLFKLKSELQFTLLF
                     VTLLLSIEIIITCFTILNYLERNKFIHAVQRINGFDFYKRHSKICFLLSLFWLIIYLGA
                     LTFKLATYTIISLIIPTYWLVELLIVYFIIRFVEKKKNKDVLKGA"
     misc_feature    complement(5493..5513)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.98"
                     /tool="antismash"
     gene            7691..8317
                     /locus_tag="HB668_RS19185"
                     /old_locus_tag="HB668_19185"
     CDS             7691..8317
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402086.1"
                     /locus_tag="HB668_RS19185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19185"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000402084.1"
                     /transl_table=11
                     /translation="MEIELKNISKKYENKQVLDNFNLVVNKGEMMAITGQSGTGKSTLL
                     NIVGLLEEPDSGDVIIQGIENAWKSEKKQIELFRYTIGYLFQNYALIDNETVSKNLDVA
                     LEYVKLPNKDDKKKEVLEKVGLLDKLNSKIYQLSGGEQQRIALARLMLKKNDIILADEP
                     TGSLDEVNRDQVLSILKSLNNEGKTILIVTHDPEVSKICTNVVTL"
     misc_feature    8530..8541
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(9092..9105)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.07"
                     /tool="antismash"
     gene            9143..10264
                     /gene="tnpB"
                     /locus_tag="HB668_RS19190"
                     /old_locus_tag="HB668_19190"
     CDS             9143..10264
                     /codon_start=1
                     /gene="tnpB"
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090861.1"
                     /locus_tag="HB668_RS19190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HB668_19190"
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_168387782.1"
                     /transl_table=11
                     /translation="MMINKAYKFRIYPNKAQTTLINKTIGCSRFVFNHFLSLWDHAYKE
                     TGKGLTYGTCSAKLPAMKKEFVWLKEVDSIAIQSSVRNLADAYTRFFKKQNSAPCFKSK
                     KNNVQSYTTKQTNENIAVIGNKIKLPKLGLVRFAKSREVKGRIVNATVRRNPSDRYFVS
                     LLVETEVQELPKIHSYIGMDVGIKDFAILSDGTTYKNPKFFRSLEEKLAKAQRILSRRM
                     KGSSRWNKQRVKVARIHEYMTNARKDYLDKISTEIIKNHDVIGIEDLQVSNMLKNHKLA
                     KAISEVSWSQFRIMLEYKAKWYGKQVIVVSKTFASSQLCSCCGYQNKDVKNLNLRKWDC
                     PSCRTHHDRDINASINLKNEAIRLLTARTAEIA"
ORIGIN
        1 accgccagta ttttggaaca accaagcttc ttaaatgaaa agatttctag cgtacgatat
       61 tcgcctttag gctaggaaga tattaatttc ttaggagaat ataagtttgg gagattacac
      121 ctaataagtt tacattgctt atgtcatttg aatattaaag agccgatttg ctcataacga
      181 ggtaaattgg gctcttttct tctcttaata ttcttagctt ggtttttttc taaaataaac
      241 gttaattatg aagagagaat gccgatgctt agaaaatgca gaaatataag gagcttatta
      301 ttaattctta ctttgctaat tatagcttgt gtattacagg ccttgggaga ggagaatggg
      361 gaatgacgga tgcaattata gaaattttta tgaaattcat tgtgatttat tgtataggct
      421 gttttggtgg tagtttgtta tggaaaacga aaacagattg aaagataagg agattcacta
      481 tgagccttcc cacccaaaaa aagatgttgc agcgggttaa aaaagaggca acgtcatact
      541 gtaatgttga tcctttagct tttgcaatag ggattagaaa tggattttta atggtaatgc
      601 cattttggtt agttgtattg tatttagtta tataaaaacg ccataactac gaaagggtgg
      661 gagaaatgat tgcttcgaat ggaattatag atatccattc gaaaaagcag gcccaagaat
      721 tcacaatgga aatgacaaaa caaatgcttc atgaaaccgt tggattcaag tgggggagaa
      781 atgcataatc atttttaagt ggaaccatct ttgatattaa agctaagtat aaagaacaag
      841 ctcatttatc cgaacttaaa gtgtttgttg tacaaagtga gaagaaggac agctttttag
      901 aagatatcac tagccatttg agattatcct tcctctcatt catgcttttt aaatcatgtt
      961 agcttgaaga atcaatacgt gctctttgtt cctatccttt ctggatcgta tatgagggca
     1021 atagaggggg gaataacaaa atgagtttaa ttactccaca ttatttgctt gtaggtggat
     1081 gttcgaaaga caaagtgtta caggcacata aaaaagcaaa agaaatattt aatcagaata
     1141 acgaaataaa taaattgata agtccgttga aaagcgactc cttttttata ctttatgatg
     1201 gcagccatca cagatggaaa aatgaagatg aatatatgaa ggcgaaaacg gcatatattc
     1261 aataccttgt agagtctgat attcagtttg tagaaatggc aacacaagaa ttaatttctt
     1321 gaaagcatgc aaaaggtcca aaaaaactag aacagcacat ctatcatgta gaaagaaaaa
     1381 aacggcgcat cattaatcag ctaatcgatt ggggagtttc aagaggctca actttattca
     1441 ggtgaatcag aagagagctt gtattatcta gtcaaagcgt tttgaggtgt atataaaaac
     1501 ctgggaattt aagagcaaac gatgaaaaat atttcatttt cacgtaataa attatgtggt
     1561 ggtagagttg acaggagaat gagattaagg acgaaaaagt cttatcacct aattaatata
     1621 gggcgaataa actagggata ccactccatg tccaccaact taagaatctt tactagttcc
     1681 acgttattct ttctaaaagg ctaaatagaa gggatgacag ttttttacta gatttttaag
     1741 ttgatggaca tatatagtga ctcctaggaa ttgatacata taaaaaagag ctcatgtata
     1801 tacaattttt taaaaataaa ctactagaat cttagaactt atatcaaatc tatagccgtt
     1861 tatcaacaag agtaaaggag cggtgagtat gacaacaatt ttagtattag aagatgatat
     1921 aactatccgt agttttatta cattgaatat gaagcgtgct ggtttcaatg ttttagaaac
     1981 cgatacaggt gaaaaagctc ttgagctttt agaagagcat aatgttgata ttgtaatact
     2041 tgatgttatg ttaccaggaa tagatggatt tcgggtatgc aaaagcatac gaaaaaacaa
     2101 tgaagatatt ggtattatta tattaacagg acttgttcaa gagaaagatc aggtacacgg
     2161 tttgacaatt ggggcagatg attatattaa aaagccattt agtataattg aattagtagc
     2221 acgtgtacaa tcattgctaa gaagaattca aaaaaataaa aaggatttgg aattgattca
     2281 ttcggggcca tttcaattaa atcttctgct aggagaatta tataggaatg agatagtaat
     2341 tgatttaact ccaacagagc atattatttt acgatattta atggaaaatt ccatgaaacc
     2401 aatttcacgt aatgaactct taaataaaat atggggtata aattgtgtag gtaatacaaa
     2461 agtcatcgat gtgaatatca atcgtcttcg tcagaaaatc gaaactagtc cttctaatcc
     2521 tgagtttctc cttacggtac gaggaaaagg atatagatgg aacgaaaaca aacattgcaa
     2581 ctaatttggc agaaaatcga ttcgcttata ctttttttta attacaaaag tatttacaaa
     2641 acttacaaaa actattttta ctattgaaag cattttaata ttagtgttaa gaaaacaagt
     2701 tttttgtgga tagcttgttt tctttcaccc ttacagaacc gtcatcgtta tgtaaggtta
     2761 ttagatagtt aatttagctt ttaaatttaa taataggatt atagcaatca acaataactt
     2821 tttaaaaact ataggagtgt gttaataatg atcgtaacaa caactaatac tattcagggt
     2881 aaagaaatta ttgagtatat agacatcgta aacggtgaag caattatggg tgcaaatatc
     2941 gtacgcgaca tcttcgcttc tgttcgtgac gttgtaggtg gccgttctgg tgcttatgaa
     3001 agtaaattaa aagaagctcg tgacattgcc atggaagaaa tgaaacaact tgcaacgcaa
     3061 aaaggagcaa atgcaattgt tggtatcgat gtagactacg aagtcgttcg tgatggaatg
     3121 ctaatggttg cagtaagcgg tacagctgta cgtgtctaaa tagaaagcaa aatataacct
     3181 aattggtgca ctgcaattct aattgttaga cataactaac aattagagtt gcagtttttt
     3241 atagaaaaat actctttgaa aaaaattagt tacatagagg ttctggcgaa tagctaacaa
     3301 tgtatcaaat aagtcgatac attaggtgta gggtttacac aatcgctatt aactattgga
     3361 atttctggca tatcccaaaa aggattcttg cgaaagtaca tgaagggagt caattcctac
     3421 tactagaaac cactgattcg gttaagcaaa gttacttagg taagaaacca tcaaagatga
     3481 tcttggaatt atgtcttata tgaaaaagcc aaatgcaaca ttttcagatg aattaaagga
     3541 agtaaaaaaa agagaataag agtgcctcaa gcataccagg gtatgttgaa aaaataaaaa
     3601 actaattaaa tcaaaaagat gatgcagaat aaattattaa tattataaac atatatcgat
     3661 tcaaaaggag atgtttagtt tgttaaatta tgaaaaagac gcagaagttt ttaaggcatt
     3721 agctcatcca gtaagaattg aaattttgaa agaattaatt ataagagggg catgtaatgt
     3781 aacacaaatt gtaggtatac tgggtattcc acaatctact gtttcacagc atttagcaaa
     3841 gatgaaatct caaaaattaa ttagaaatga tagaaaagga ttagaagtct actatagtgc
     3901 ccaaaacaaa actattaatt ccattgttga aatactacta gattacaaat ctactaattg
     3961 taagtaaaaa ataataatct ttgttccaga accccctacg tcaatcaagg attcttagcg
     4021 gattgatgag acatatgtaa aaattaaagg tcaatggatg tactaatacc gtgctgttat
     4081 ttaaagttaa tacaattaat tttcatgttt caaatacgtc aatgactcct tatgattaaa
     4141 ctgcccccac tttcacttct tttagaagag ggggattcct aagtaaagag ttaggctatc
     4201 tccacagtcc ttgcggttag aagccttatc gcttcattct ttagattgat acttgcgtta
     4261 taaacatttt cagtcttcct tctttttaat taactctttt acaaaacctt ttactttctc
     4321 tttttctacc tcactacatt ttctttcttc aaattcagtt aggtttatat ggatctcttg
     4381 taatagttca taaatatctt tcatactatc ccacctccta aattctttct tattttaatt
     4441 ttttatgcaa ttaagaaagt gaatctaagg cgtatttagt aaaaatttat tatttacata
     4501 ttatagaaat atttcatttg taatatgtaa cattaataat ggatagacaa tttcttatac
     4561 cttatataac gtattaaaca atgtgatttc tcacacttac ctcaaatttt tttaaattgt
     4621 attatttaaa acactttcca attactcccc tctatatttg taaatattta cttgattgaa
     4681 aattttattt ttaagtatca ctatttttat atcaaataaa tactgaaaat tcagaatttt
     4741 atttaaaaaa ttttgaatat gtctttcttg aaaaaaactg tgataatatt aaaaaaaacg
     4801 atttgaagtt aatatttccc accctatttt taattgaatt attttttcat ttgagtatct
     4861 ttaccttatt tacttcctat ttgttagttt attgtattct gttctatata tttatttttt
     4921 aatgcggttg tttaaaaata aaaaggtagt cagtctgcaa tatctaaata aattaattag
     4981 atgttaaagg gggaaaaaaa ttgaaattga aaaaaactgt aagttcatta gtattaagtg
     5041 ctgcaatact tggttcagga gcaacagcag catttgctgc aacacaaaat gtaggggggg
     5101 gaacatggaa ttatggaacc ggtggtggtt cagtttggtc aaattattac cacggaagca
     5161 aagttcacaa atcttcagta atcggacaat attattatag ttctccatgg acgaacccag
     5221 gttacacttc atatgcaagt gcacctgata cagctggtgt agatgaatct tattggtctg
     5281 ttaaataaaa agtaagaaaa atcctagaac tcgtgggtta ttacctgcat gttctaggat
     5341 tctattttat gttacttata tacatataga taagtaaatt atttttatta atatttgaag
     5401 acaaaggata ttctatcttc atttgaattc aaaatgaaaa tagaatacgg aaaatggaga
     5461 ctggtatgaa aaaaatatta attactttag tacttgtagc ctatgctgta tcttcatttg
     5521 tatttactaa gcactttgaa aaaagagaaa tggtaaatat taatttttct gatagaaatg
     5581 gaattcaagt ggttgaattg aataaagatg aacgtccgga agataaactg agaaagattg
     5641 aggaattatc caaaaaggaa aaaatcaaca tctataaatt atcatataaa ccgattaaaa
     5701 cttcaaaaaa acaaaaagta attatctatg cagctatagg ggatgaaaca caattcaaac
     5761 aaaaatatcc actagaatgg ggaaagatat tcaatcaaga tcattccgta gcaagttatt
     5821 tgtcatcaag cgatacgaaa gataaagagc aggttggaaa gataaacata ttttcacctg
     5881 aacttatggt agaaattaaa cctatagaag cggcaaaaga tgaaaacttg agaggcgctt
     5941 attttgttga tagtaacaat ccaaacattg ttcaaaaggt aaaagagaaa ttaaataccg
     6001 aaatcgggtt caaaacggaa gagtcagaac gagcaggtgc tatgaacatc ctagaaaatc
     6061 agggagttga taagtacttt attatagttc ttactatagt tctgttactc atcttcctga
     6121 gttttttata ttatatatta ttaaattata aaagtttggc aattagaaaa ctgtttggat
     6181 atacagatag agaactgatt tttaagcatc tattaaaaga aagtattcaa attcatacgt
     6241 tatccttaat ggtagttatt ctaggacaag taatatactt gtttttttat aatgggatga
     6301 gtaaattctt agattttggt agtacatgga ttgtgtggca attaattttt tcaggtattt
     6361 ctattctagt tagcctactt ccttctttaa tgatatatca tataaaaatt ttggaactat
     6421 taaaaaataa aaaaccatta aaactagttc aatttttaaa ttatagttca aagtttatct
     6481 tttcgatttt tctaattatc ttttctttta atcttataaa ttattatcaa gatctatctg
     6541 tacaaaactc taaccaggaa caatgggata caacaaaaaa ttatagtttt tatgaatatc
     6601 aaagtaatat tataggcgac cgcacaaact gggaatatga aactggaatt aaaagcaaga
     6661 aactatttgg aataactaac caaaaagggg ctttgcttat aaaaccatca gatggcataa
     6721 cgtacaaaaa acagttagag tataacaatt ctttaaacaa tactatagga gatgttaaag
     6781 agtatgatcc tcacggcggt aacacccttc aagtgaattc taattattta catgataatc
     6841 cagtttatga tttgaatggt aaaaaaatca atatcgatgc tgattatggt gattatttag
     6901 ttatattagt tcccaataaa tatcgcagca atgaagctga tattttaaaa atgtataaag
     6961 aatggtatca attcacaagg tttattgatg aagataaaca taaggaaagc ataggagaaa
     7021 aaattgaacc acataaacag gttgaaatta aacttaccta tatccaagat aaccaaaaac
     7081 attttttgta taatcctatg ctagaggtta aaaaccaaaa ttatagcaat ggcgctatat
     7141 tgattgttgt taactcagaa aatatgggtg gagacagcta tttaagttat ttaactgggc
     7201 gttatttttt cccaaaagta gataatctaa gccagcctta taaagaatta aaagaagaca
     7261 ttaaaatcgc aggtcttcaa gatatcattc ttacaacgcc gtcattatat agtattgtag
     7321 atgattatct ctttaaatta aaaagcgaac ttcaatttac tcttcttttt gtaacattat
     7381 tacttagcat agagattatt attacttgtt ttacaatttt aaattattta gaaagaaata
     7441 aatttattca tgccgttcaa agaatcaatg gctttgattt ttataagcga cacagtaaaa
     7501 tctgttttct cttaagtctt ttttggttaa taatttattt aggtgcatta acatttaaat
     7561 tagcaactta tacaattatt tcacttataa ttccaactta ttggttagtt gaattattaa
     7621 ttgtttattt catcattcga tttgtagaaa agaagaaaaa taaggacgta ttaaaaggag
     7681 cgtagttcaa gtggaaattg agctaaaaaa tatatctaag aaatatgaaa ataaacaagt
     7741 gcttgataat tttaatttgg ttgtaaataa aggagaaatg atggctatta cgggtcaaag
     7801 tggaaccgga aaatcaacgc tattaaatat agtaggtctt ttagaagaac cagactctgg
     7861 ggatgtaatc atacaaggaa tagaaaacgc ttggaagagc gagaaaaaac aaatcgagct
     7921 tttcagatat acaataggct atctctttca aaattatgca ttaattgata atgagactgt
     7981 gagcaaaaat ttagatgtag cactagaata tgttaagtta cctaataaag atgacaagaa
     8041 aaaagaagtt ctagaaaaag ttgggctact agacaaatta aacagtaaaa tttatcaact
     8101 tagtggtgga gaacaacaaa gaatagcatt agctaggctc atgttaaaaa agaatgatat
     8161 catcctagca gatgagccaa ctggatcatt agatgaagtg aatcgggatc aggtgcttag
     8221 tatattaaag tcattaaata atgaaggaaa aacaattcta attgttactc atgaccctga
     8281 agtgtctaaa atatgtacta acgttgttac actataaatt ttgaaaataa ggtattaacg
     8341 gttatggtgc aaaaatttca aaataactgt aatgaaaaat cccccataga tcttatacct
     8401 atgagagctg cctaagggta tttcatattt gacttagttc tattgtaaca ggctttcgcc
     8461 atacatgaga aacataaaga cccaaaaact aacaatcata acaaaaaatt ctttacacct
     8521 ttgctgaaag gaaatgtacg cttatcttat aaaaaagaat gctcctttaa ataacaaaaa
     8581 gggcattctt tttttatgga gtctttattc tccagtatcc aacttcattt taaatgaggc
     8641 tttagccttt tctggtagat gtcctttaac tccgagtatg aaaggtttcg tttttgtgca
     8701 actatttgaa ttaacgcaat tttgtgataa acctaaaacc acatgcccat caacttaaga
     8761 aagtaaatac cccaaaacga gaaaattggc atctccacat aaaaatatac aatttttttg
     8821 ttttggggat gcttcaaaat attagcttga tagcaatgga attcgtacat atgaataata
     8881 aacaaacaga tttcagtttc taattcatat tagaagtaat gtacctattt cttctgtact
     8941 taaaccattt aattttatca ttttagccaa gaagactcct tcctcaagga acgcgaattg
     9001 agtaggtggg agatgaattg gcttcgaaca agggatggca ggcagccatc ttaattgttc
     9061 gacatacata aactgttact tcactaccta tcgaatatat gtttgttgta taatagacac
     9121 atatcgaaat ggggtgaaac gaatgatgat caataaagca tataaatttc gtatctatcc
     9181 aaataaagca caaacgaccc taatcaataa aacgattggc tgttctcgtt ttgtattcaa
     9241 tcatttccta tctctatggg atcatgcata caaagaaaca ggaaaaggtt tgacatatgg
     9301 tacatgctct gccaaacttc ctgccatgaa gaaagagttt gtttggctaa aagaagtgga
     9361 tagtattgcg attcagtcgt ctgttcgcaa ccttgcggat gcttatacac gctttttcaa
     9421 aaaacaaaac agtgccccgt gtttcaaatc taagaaaaac aacgtacaat cttacaccac
     9481 aaaacaaaca aatgaaaaca ttgccgttat aggaaacaaa attaagttgc caaaactagg
     9541 tcttgttcga tttgccaaaa gtcgtgaagt aaagggacgt attgtaaatg ctacagttag
     9601 acggaaccct tctgacagat actttgtatc attgttagtt gaaacagaag tgcaagaact
     9661 tccgaaaata cattcttaca ttggaatgga tgtgggaata aaagatttcg ccattttgtc
     9721 agatggaaca acctataaaa atccgaagtt ttttcgatca ttagaagaga agttggcgaa
     9781 agcacagcgt attctttcta gaagaatgaa aggatcttct cgatggaata aacaacgggt
     9841 aaaagtagct agaattcatg aatacatgac gaatgctaga aaagattact tggacaaaat
     9901 ctcgactgaa atcatcaaaa atcacgatgt tatcggtata gaggatttgc aagtatcgaa
     9961 tatgttaaag aatcataagt tagcaaaagc aattagtgaa gtatcatggt cacagtttcg
    10021 aatcatgttg gaatacaaag caaaatggta cggcaaacaa gtcattgtcg tatcgaaaac
    10081 atttgcttcc agccaattat gttcttgttg tggatatcaa aacaaagacg ttaaaaatct
    10141 aaacctacgt aaatgggact gcccttcttg tcgtacacac catgataggg atattaacgc
    10201 aagtatcaat ctaaagaatg aagcgataag gcttctaacc gcaaggactg cggagatagc
    10261 ctaataaatt agagttcgat agaactct
//
