LOCUS       NZ_CP040605            22698 bp    DNA     circular CON 25-AUG-2024
DEFINITION  Saccharopolyspora sp. ASAGF58 chromosome, complete genome.
ACCESSION   NZ_CP040605
VERSION     NZ_CP040605.1
KEYWORDS    RefSeq.
SOURCE      Saccharopolyspora sp. ASAGF58
  ORGANISM  Saccharopolyspora sp. ASAGF58
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 8045912)
  AUTHORS   Guo,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2019) Grain Processing Research Group, Academy of
            National Food and Strategic Reserves Administration, Baiwanzhuang,
            Beijing 100037, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.15x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012277335.1-RS_2024_08_25
            Annotation Date                   :: 08/25/2024 04:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,606
            CDSs (total)                      :: 7,535
            Genes (coding)                    :: 7,265
            CDSs (with protein)               :: 7,265
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 228 of 270
            Pseudo Genes (internal stop)      :: 14 of 270
            Pseudo Genes (multiple problems)  :: 48 of 270
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:54:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6296808
            Orig. end                         :: 6319506
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040605.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22698
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="22"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22698
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22698
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12698](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12698
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            259..888
                     /locus_tag="FDZ84_RS29355"
                     /old_locus_tag="FDZ84_29480"
     CDS             259..888
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013122.5"
                     /locus_tag="FDZ84_RS29355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29480"
                     /product="PolC-type DNA polymerase III"
                     /protein_id="WP_210435202.1"
                     /transl_table=11
                     /translation="MVIDFETTTPAGHPAQPIEVAALALRYQEGAWTAVGRSSSLIRPP
                     AFAPVTPASTAQTGLTPEQLRQAPTPAEALGALDRRFTADNRYLLVAQHAATEANIIHN
                     QREHCPALARVDFLDTIPFAKHLVPGLPNYKLDTLLAHFSIQHPADRHRAYADVDVTAQ
                     VFLRLVNTADENAQPSDLAALVTIAGRTAKCNIQCNIPVQSGLFEI"
     gene            942..1580
                     /locus_tag="FDZ84_RS29360"
                     /old_locus_tag="FDZ84_29485"
     CDS             942..1580
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153288.1"
                     /locus_tag="FDZ84_RS29360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29485"
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="WP_305852021.1"
                     /transl_table=11
                     /translation="MSMIKQQADRDGVPFWFDPLCPWAWITSRWLLEVEQVRPVRADWR
                     IMSLAYLNLVQREGKGLSEDYQELMVKAWGPVRVCAAAAEYGGRGVLGPLYTAIGTRLH
                     NQRRRDDPTVMTEALAAADLPDSLAAAATSTEFDQVIKDSHDEAFNEVGLDVGTPVLRI
                     GGTALFGPVITPAPRGEAAGQLWDGLVMVAATDGFFELKRSRDRKPSFE"
     gene            complement(1732..2292)
                     /locus_tag="FDZ84_RS29365"
                     /old_locus_tag="FDZ84_29490"
     CDS             complement(1732..2292)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634935.1"
                     /locus_tag="FDZ84_RS29365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29490"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_168588507.1"
                     /transl_table=11
                     /translation="MAISDGDISSVLAAYLECYPEEAALLSEPARLLSQGGDFASRRSF
                     PMHVTVGALLVRGDAEILLVEHRAYGITLQPGGHLEPTDTALIDAAARELTEETGVDPD
                     RVFPALQTPVYIEYGPVPARPEKDEPEHYHLDIGYSFTTAHADVGRIQESEVTGAAWYP
                     LTAAERLVGNRIARAVSAPARIG"
     gene            complement(2528..2821)
                     /locus_tag="FDZ84_RS29370"
                     /old_locus_tag="FDZ84_29495"
     CDS             complement(2528..2821)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29495"
                     /product="hypothetical protein"
                     /protein_id="WP_168588508.1"
                     /transl_table=11
                     /translation="MDDSDCDTAPAQYTGSTPVLDVHPSPQRFWYYYRANGQTAPKVGD
                     RITGGGTFTTPVAAPGEPAPVIGRKGSIPVSGGPVQRGGFGINTNPGTTSGT"
     misc_feature    2795..2805
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    2841..2854
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     gene            complement(3385..3570)
                     /locus_tag="FDZ84_RS29375"
                     /old_locus_tag="FDZ84_29500"
     CDS             complement(3385..3570)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29500"
                     /product="hypothetical protein"
                     /protein_id="WP_168588509.1"
                     /transl_table=11
                     /translation="MLEKAIGAVIFVMVLCVGILALVAQVRMNMGEFYSFARAFSATTY
                     DAAQLIAPIGALLQKR"
     misc_feature    complement(3397..3399)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3741..3755
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.61"
                     /tool="antismash"
     gene            complement(3744..4367)
                     /locus_tag="FDZ84_RS29380"
                     /old_locus_tag="FDZ84_29505"
     CDS             complement(3744..4367)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="FDZ84_RS29380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29505"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_168588510.1"
                     /transl_table=11
                     /translation="MTGMHIHKRPSWKPDEWGPSQLSRLTPARRRIALSLLRDYNIDET
                     ARDTYLSAYTVKTHLCRIRRDLRARDRTHLVAALYETGFAFPSWAHQKVNVTYMRAVFD
                     APFNLIDTPFDPGQVNLLHDREIRTIKLVTQGLTNHAIAKLEYIEEDSVKSRLKEIRNK
                     LNLKNRHYFSVFAYESGLEHVELTLTRSSNRPAPAGRGTLPISK"
     misc_feature    complement(3825..3827)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4215..4217)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4403..>4546)
                     /locus_tag="FDZ84_RS29385"
                     /old_locus_tag="FDZ84_29510"
                     /pseudo=""
     CDS             complement(4403..>4546)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /locus_tag="FDZ84_RS29385"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29510"
                     /product="helix-turn-helix domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KENAQYVGASRETVNKALADFARRGWLRLEGKSVLILDPERLARR
                     CH"
     gene            complement(4504..4854)
                     /locus_tag="FDZ84_RS29390"
                     /old_locus_tag="FDZ84_29515"
     CDS             complement(4504..4854)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982421.1"
                     /locus_tag="FDZ84_RS29390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29515"
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="WP_246869248.1"
                     /transl_table=11
                     /translation="MTGNRGYHTSLSRPGDQGAAPCRTVQYSCPQSHKFAVPFSGDADV
                     PPTWECRMHGTASKRIDGSEPEQKKAKPPRTHWDVLLERRTIPELDELLHQRLAALRGK
                     KTRSTSAPRGRP"
     gene            complement(4860..5372)
                     /locus_tag="FDZ84_RS29395"
                     /old_locus_tag="FDZ84_29520"
     CDS             complement(4860..5372)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29520"
                     /product="hypothetical protein"
                     /protein_id="WP_168588512.1"
                     /transl_table=11
                     /translation="MNRLLPSLPRSFSEEAAEIETVEWVVSPSESVVHCRLPGTGDLAC
                     GHKLADELETCTGYLPAARWPRYQPHLACLASAPTWVDDLVEPRALLPYAEESVSGRHA
                     IETVSTLRIPNLVRHIKALELSMTGPPTRTGTENVPGSSYLPGTCRKSSDTSVVECTTL
                     HELGEVA"
     gene            complement(5369..5500)
                     /locus_tag="FDZ84_RS40175"
     CDS             complement(5369..5500)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS40175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_256413586.1"
                     /transl_table=11
                     /translation="MNDLAFTLLVVVGLAGALGAGLWWLITSPAETETQHLFAEGKE"
     gene            complement(5497..5706)
                     /locus_tag="FDZ84_RS29400"
                     /old_locus_tag="FDZ84_29525"
     CDS             complement(5497..5706)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29525"
                     /product="hypothetical protein"
                     /protein_id="WP_168588513.1"
                     /transl_table=11
                     /translation="MLNELRAVTCRDRTGQDRDVVVGHTPSTVWLRVAGEERLLDTTQA
                     EALYVAIGVQIAAVQSARRKAVGA"
     gene            complement(5815..6228)
                     /locus_tag="FDZ84_RS29405"
                     /old_locus_tag="FDZ84_29530"
     CDS             complement(5815..6228)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF027681.5"
                     /locus_tag="FDZ84_RS29405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29530"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_168588514.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 9e-12, bitscore:
                     43.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPPQLPALDRQLGGRRVLVTGGSGFIGSRVVEELCRPGAQVVSV
                     DRRRPWWRSPVSCRVEECDIRTNRLAELIVEFAPHVVVHLAAQVGVPESVRCPAVDAEV
                     NVRGTITVAEAAAAFHRDVSSRFMPWFQHRSPE"
     gene            6515..7873
                     /locus_tag="FDZ84_RS29410"
                     /old_locus_tag="FDZ84_29535"
     CDS             6515..7873
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696427.1"
                     /locus_tag="FDZ84_RS29410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29535"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_168588515.1"
                     /transl_table=11
                     /translation="MGAGRTSWAASEAPGELIRRKRKEKGLTLVQLGKLAGFSAAQVSR
                     YERGVSALTDVAVLRSFADALGIAPQALGLTSGPAGNGHAIAPADYPRLPTSKVQERSG
                     REDGEDPVRRRQLLANLAVTAATAAGAPLLLTTTGGPVSEAALGPVLITRLRDAMLGLH
                     PGTDNPLPPEVDLTRAYTDFRACRYGSLATRLPRLLHAGHAHAGGDGNTLLAQTYLLVV
                     RVLIKLDDQQLGWMAADRARQYAEATGDALFVAEAARHLAVLARKARWYEQAMNIALTA
                     AEHPELRPGGPEHAAGRGLLIQSAAYTAAKCGDAAGMRELTTEAATIADGLGRTLLPDH
                     GGFSTTTVRLHLISAELSAGDPAAALTAARMVAPQTLPSTERRARYYTDVATAFAQWGR
                     REQCLRALLTAESHAPEETHLRPAVKSLVTGLLVSGHTTAELRGLAARVGVPA"
     gene            complement(8012..8527)
                     /locus_tag="FDZ84_RS29415"
                     /old_locus_tag="FDZ84_29540"
     CDS             complement(8012..8527)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310046.1"
                     /locus_tag="FDZ84_RS29415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29540"
                     /product="integrase core domain-containing protein"
                     /protein_id="WP_168588516.1"
                     /transl_table=11
                     /translation="MEISVPRDRDGSFEPAIVKKRQRRLSGVDDLVISLSQVRNLLMDL
                     GDDVTEFRFLIRDRAGQFTASFDAILADVDIRVAKTPPHCLRANCFAERFVRTVRAELT
                     DRMLIFGERHLRAVLAEYVRHYNGRRPHRARELRPPRSSHPVANLSHKRIKTRPVLDGL
                     INEYERAA"
     gene            complement(8625..8813)
                     /locus_tag="FDZ84_RS29420"
                     /old_locus_tag="FDZ84_29545"
     CDS             complement(8625..8813)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29545"
                     /product="transposase"
                     /protein_id="WP_168588517.1"
                     /transl_table=11
                     /translation="MTEVKAEQASEAAAADLAVMDEQIQQLADRARAEGLRLTGEGGLL
                     GRLTKLVTEAALAGEME"
     gene            complement(9177..9815)
                     /gene="mftR"
                     /locus_tag="FDZ84_RS29425"
                     /old_locus_tag="FDZ84_29550"
     CDS             complement(9177..9815)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152874.1"
                     /locus_tag="FDZ84_RS29425"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="FDZ84_29550"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_168588518.1"
                     /transl_table=11
                     /translation="MERIPLAHNTADKNTRQVGRRKVTTAAELEHIAFELFDEQGFDST
                     TITDIAQAAGISRRTFFHYFPSKNDIPWGDFDHELARMREDFRALSANTPLMDAIRHCL
                     VNFNQVAPEQISAHRRRMQLILGVPALQAHSTLRFRAWRQVIAEYVGTRLGQPDNALAP
                     QTIAHATLGVALAAYEQWLNEDESELTELLDTAMRQLGTAFADISPAQD"
     gene            9894..10001
                     /gene="mftA"
                     /locus_tag="FDZ84_RS29430"
                     /old_locus_tag="FDZ84_29555"
     CDS             9894..10001
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947188.1"
                     /locus_tag="FDZ84_RS29430"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29555"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_168588519.1"
                     /transl_table=11
                     /translation="MADEMREASTVSAEPAIEEELLVEEVSIDGMCGVY"
     gene            10001..10273
                     /gene="mftB"
                     /locus_tag="FDZ84_RS29435"
                     /old_locus_tag="FDZ84_29560"
     CDS             10001..10273
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310366.1"
                     /locus_tag="FDZ84_RS29435"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="FDZ84_29560"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_168588520.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.8e-24,
                     bitscore: 84.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAAVEFDTSLGYRLNPQVALRPEPFGALAYHFGNRKLSFLKVPE
                     LVDLVRGLADHASVEEALQEVSEGRRAQFRRALASLAATDMIRPR"
     aSDomain        10034..10261
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_FDZ84_RS29435_Mycofactocin_RRE.1"
                     /evalue="6.80E-27"
                     /identifier="RREFam016.1"
                     /label="FDZ84_RS29435"
                     /locus_tag="FDZ84_RS29435"
                     /protein_end="87"
                     /protein_start="11"
                     /score="84.5"
                     /tool="antismash"
                     /translation="GYRLNPQVALRPEPFGALAYHFGNRKLSFLKVPELVDLVRGLADH
                     ASVEEALQEVSEGRRAQFRRALASLAATDMI"
     gene            10303..11496
                     /gene="mftC"
                     /locus_tag="FDZ84_RS29440"
                     /old_locus_tag="FDZ84_29565"
     CDS             10303..11496
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310365.1"
                     /locus_tag="FDZ84_RS29440"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="FDZ84_29565"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_168588521.1"
                     /sec_met_domain="PTHR43273 (E-value: 2.7e-40, bitscore:
                     137.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 3.5e-06, bitscore: 26.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4.5e-26, bitscore: 91.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.4e-07, bitscore: 30.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.9e-14, bitscore:
                     52.6, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 4.7e-05, bitscore: 22.6,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTAVCEPAPVPALVEQFKGGLDAPICLTWEWTYACNLACEHCLS
                     SSGRRDPRELSTAEMKAVIDELQRMQVFYVNVGGGEPTVRPDFWELLDYAVEHQVGVKF
                     STNGLRIDRERARQLAATDYVDVQISLDGATREVNDAVRGEGSFDMAMRALENLSEAGM
                     RDFKISVVMTRQNVSQLDEFKVIADRFGAQLRITRLRPSGRGADVWDELHPTDAQQHEI
                     YSWLVEHGDQVLTGDSFFHLNALGSTPLPGLNLCGAGRVVCLIDPVGDVYACPFAIHDA
                     FKAGNVREPGGFGSVWRESTLFEDLRRPQSGGACQSCSAFDACQGGCMAAKFFTGLPLD
                     GPDPECVKGHGQKALSVVDRSGLPKASQNHSRASRRKVSLGMPGVPAKPCDSSPLAS"
     gene            11523..12698
                     /gene="mftD"
                     /locus_tag="FDZ84_RS29445"
                     /old_locus_tag="FDZ84_29570"
     CDS             11523..12698
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947184.1"
                     /locus_tag="FDZ84_RS29445"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="FDZ84_29570"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_168588522.1"
                     /sec_met_domain="TIGR03966 (E-value: 3.7e-213, bitscore:
                     706.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASTRQWFETVAEARERARKRLPKSVYLALLAGSERRTTLDDNVE
                     AFAELGFRPRIADLPAMRSQTIEVLGQELSLPVILSPTGVQAVHPDGEIAVAKAAAASG
                     TAVGLSSFASKPVEDVVAANPKTFFQAYWLGSRESIQQRLDRAKRAGAKGLIVTLDWTF
                     ATARDWGSPPLPERVDIKTMLKFAPQVLARPGWLVQYLRSGSLPDLTTPNLVDPEEKGP
                     TFFGAYGEWMQTAPPSWEDLAWLREQWDGPFLLKGITHPDDARRAVDIGATAISVSNHG
                     GNNLDSTPATIRVLPSIVDTVGDQIEVLLDGGIRRGSDVVKALAMGARAVMIGRAYLWG
                     MAAGGERGVHNVIEILRSGIDSALLGLAKSSVHEVDRGDLVIPDGFTRGMR"
     gene            12796..13491
                     /locus_tag="FDZ84_RS29450"
                     /old_locus_tag="FDZ84_29575"
     CDS             12796..13491
                     /NRPS_PKS="Domain: PKS_KR (32-148). E-value: 2.4e-09.
                     Score: 29.3. Matches aSDomain:
                     nrpspksdomains_FDZ84_RS29450_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR04504.1"
                     /locus_tag="FDZ84_RS29450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29575"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_246868519.1"
                     /transl_table=11
                     /translation="MAVDRCSDDKRVPYHLATREELAALVGEFPGAVTDVVADVQDRAA
                     LADAVAVAEHEFGGLDAAVACAAVMAGGQPLWEADDDEWQVLFSVCVDGVANLARVAVP
                     SMLRHPEPRTGRFVALASAAGHRGLLNLAGYNAAKHAVVGLIKGLAHDLRGTGIGATAV
                     SPGSTRTAMLDATAQVYGLESTEELAQHQLVGRLLEPDEVAAAICWLCSADSSAVSGSV
                     IHADGGFTA"
     aSDomain        12892..13239
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FDZ84_RS29450_PKS_KR.1"
                     /evalue="2.40E-09"
                     /label="FDZ84_RS29450_PKS_KR.1"
                     /locus_tag="FDZ84_RS29450"
                     /protein_end="148"
                     /protein_start="32"
                     /score="29.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VTDVVADVQDRAALADAVAVAEHEFGGLDAAVACAAVMAGGQPLW
                     EADDDEWQVLFSVCVDGVANLARVAVPSMLRHPEPRTGRFVALASAAGHRGLLNLAGYN
                     AAKHAVVGLIKG"
     gene            13565..13837
                     /locus_tag="FDZ84_RS29455"
                     /old_locus_tag="FDZ84_29580"
     CDS             13565..13837
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014670.5"
                     /locus_tag="FDZ84_RS29455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29580"
                     /product="creatininase family protein"
                     /protein_id="WP_168588523.1"
                     /transl_table=11
                     /translation="MNLATKHWPHLAAGRGEHLLAVPLGATEQHGPHLPLGTDTTIAEE
                     LCRRLAQRMPKILVAPAIPYGSSGEHAGFPGTLSIGQEAMPLSLS"
     gene            13884..15125
                     /locus_tag="FDZ84_RS29460"
                     /old_locus_tag="FDZ84_29585"
     CDS             13884..15125
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612226.1"
                     /locus_tag="FDZ84_RS29460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29585"
                     /product="IS4 family transposase"
                     /protein_id="WP_168588524.1"
                     /transl_table=11
                     /translation="MVAGARLTDLVSLGVLASSVPRDVVDEVIVAAGRQAKRSDGKLPP
                     HVMVYFVMAMALFASEDYEEVAARLTDTLAAWGCWDQQWSLPTSGGITQARQRLGAEPL
                     AELFARVAVPVAGELTRGAFLGPWRLMAIDGFEWDAPDTKANGAEFGYGGSGESRSAFP
                     KVRVVSVSECGSHAMVDAEIGGVTGKGSGEQTLARRLYRRLQPGWLLIADRNFYTWQDW
                     CTAADTGAELLWRVKSNTRLPVLGSLYDGSYRSVLIEAKIRGRARQALIDAAARGEDLP
                     PDQARIVRVVEYTVPDRDGNGKGELIRLITTITDPTAAPAAALAESYHHRWEHETANGQ
                     IKTVLRGPGRVLRSKSPDMVRQELYGYLLTHYAISALICRAATEADIDPDRVKFTRTVR
                     IVRRRIADPAAFSP"
     misc_feature    complement(15251..15260)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     gene            <15364..15810
                     /locus_tag="FDZ84_RS29465"
                     /old_locus_tag="FDZ84_29595"
                     /pseudo=""
     CDS             <15364..15810
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014670.5"
                     /locus_tag="FDZ84_RS29465"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29595"
                     /product="creatininase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ELVLTELVRSADAFAGVILVNAHGGNLQPLQAVLRTLRSEGRRIY
                     AWMPSGANTDSHAGHTETSVMLHLQPDHVETALMRKGNTTPLPELIDRLRADGVAAVSP
                     DGVLGDPTHASADQGATILERWTEQLTTAVEKWSRIWRDASAAL"
     misc_feature    complement(16150..16164)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.19"
                     /tool="antismash"
     gene            16173..16742
                     /locus_tag="FDZ84_RS29470"
                     /old_locus_tag="FDZ84_29600"
     CDS             16173..16742
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.5"
                     /locus_tag="FDZ84_RS29470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29600"
                     /product="glycosyltransferase"
                     /protein_id="WP_246868520.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.5e-16,
                     bitscore: 57.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHPVYGDGQLRPVDVTVVVPVRDDADMVADLRMAAGDVAEVIVV
                     DDGSQIPIDGAALRNETARGPAAARNAGWRRARTALVAFLDADTALERGWLEPLLRHFE
                     DPTVVAVAPRVRSMDGDTILARYEYLRSPLDLGAVPGPVDSTWPNPERGNAVTPRVARA
                     GQGRQPSRRLPGHGLARLSCQQARSW"
     gene            complement(16888..17124)
                     /locus_tag="FDZ84_RS40180"
                     /old_locus_tag="FDZ84_29610"
     CDS             complement(16888..17124)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013584.5"
                     /locus_tag="FDZ84_RS40180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29610"
                     /product="amidase family protein"
                     /protein_id="WP_168590436.1"
                     /transl_table=11
                     /translation="MTGLPALQVPTGFTSTGLPMGVQIVGKPFAEDEGRSKRACGCSVW
                     LRRLIKPPQPPHWRLVTRTLRGFHRAPRSFVRC"
     gene            complement(17150..17353)
                     /locus_tag="FDZ84_RS40185"
                     /old_locus_tag="FDZ84_29615"
     CDS             complement(17150..17353)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS40185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29615"
                     /product="amidase family protein"
                     /protein_id="WP_168588526.1"
                     /transl_table=11
                     /translation="MLAVGEAVLATDYINTMRLRTQIQQDWCRMFDQIDVLLAPSSPGP
                     ALRRDDLYFRWDDGIVEGATSA"
     gene            17450..17767
                     /locus_tag="FDZ84_RS29490"
                     /old_locus_tag="FDZ84_29620"
     CDS             17450..17767
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="FDZ84_RS29490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29620"
                     /product="response regulator"
                     /protein_id="WP_256413587.1"
                     /transl_table=11
                     /translation="MFLGAFAALLDLQPDITVMATAGTGDEALAAVERHRPHILLTDIE
                     MPGRTGLEVAAERFTTSRRSFPGVAYTCSRRCFGHSRCSTKKFRMTILRDRLHPSPESV
                     L"
     gene            17899..19437
                     /locus_tag="FDZ84_RS29495"
                     /old_locus_tag="FDZ84_29625"
     CDS             17899..19437
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /locus_tag="FDZ84_RS29495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_29625"
                     /product="IS66 family transposase"
                     /protein_id="WP_168588527.1"
                     /transl_table=11
                     /translation="MPVPEAMTRDELIELVGEQGARIAALGEQIAVRDRQITVMATQMA
                     ELAEVNETLGERLAKLEHVLSRNSKNSSSPPSKDDGPGRTPPPAKAKRGGASKRRGKQP
                     GTPGANLAWTDSPGDHKDRFLRGVCECGTDLAGARDLGVVDRYQQHEIPPVSVKITQYD
                     QHAVRCGCGRVHTAARPEGARPGPVGYGPNLQAFAVYLMVVHFIPVHRCVELLASLTGA
                     APSVGFVHGMLTRAAGVLTEVDTRIRTLITLAYAVCCDETPLRVGPRTPKPGKKKAEKY
                     LLVACTELFTHYLLGDRDLATFKAFVIQDLAGSVIVHDRYQNYDSAELGELTHQLCCAH
                     LGRDLDGAGEVYPGAHWPTQIADALRGLIHEANLARATGRDAIEQTIKNELIKRFRDGV
                     RVGLSDTTSHGNRPGERKTRLLLEVLRDREPDVLRFAHDLKVPPTSNQAERDLRPAKVQ
                     QNISGRLTSEKRTKDRYRIRGYLSTASKHGRNMIEALREAIRGHPWMPPDPTPA"
     gene            complement(19503..19988)
                     /locus_tag="FDZ84_RS29500"
                     /old_locus_tag="FDZ84_29630"
     CDS             complement(19503..19988)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29630"
                     /product="hypothetical protein"
                     /protein_id="WP_210435203.1"
                     /transl_table=11
                     /translation="MSVSTPSDDQSGSSTPPGADPAPRPSRRVFSPEYKLAIVAEYENA
                     PNGEKGAILRREGLYSSHVIEWTRDRDAGVLTARSADAGSPPKRPKKTAEQLELEKLRR
                     ENEKLKSDLSKTRIALDIMGKAHALLEELSGSAENDEPPKSRKNREVILSKHSVGLP"
     misc_feature    complement(20118..20134)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.78"
                     /tool="antismash"
     gene            complement(20280..20714)
                     /locus_tag="FDZ84_RS29505"
                     /old_locus_tag="FDZ84_29635"
     CDS             complement(20280..20714)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS29505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_29635"
                     /product="hypothetical protein"
                     /protein_id="WP_168588529.1"
                     /transl_table=11
                     /translation="MSTSSDRPGQPAQQPDPAARPSRRVFSPEYKLAIVAEYENAPNGE
                     KGAILRREGLYSSHVIEWTRARDAGVPTARSDKAGGAAPKRAKKTAVQLELEKLRRENK
                     KLKSDLGKTRMAPPPTMASRTACTIRPSRSSGTSGQLAVP"
     misc_feature    complement(21152..21163)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.24"
                     /tool="antismash"
ORIGIN
        1 taccggtccc tgagtgaaac ctggacgggc gagcgcctct ggaacgcacc ggacctgcca
       61 gccgccacgc aggccgttac ccgactcatc agtcagtaac ggcacaccac gtcgtgacgg
      121 ctctaccacg gcggctggaa cccggcatgc tctcgtcgat gtggcgggcg cgggccggta
      181 aacgccacgg aatcaacaaa gaacggtgga tagatgggcg gcttgacaaa agaccccgct
      241 ttcctggcga cgaccttcgt ggtcatcgac ttcgagacga cgacccccgc gggccacccg
      301 gcccagccca tcgaggtcgc cgctctcgca ctccgctacc aagaaggcgc gtggacggcg
      361 gtcggcagaa gttcgtccct catccggccg cccgcgttcg ccccggtgac gccggccagc
      421 acggcccaaa ccggcctgac acccgagcag ttgaggcaag cccccacccc ggccgaggcg
      481 ctgggcgcac tggaccggcg attcacggct gacaaccggt acctgctggt ggctcaacac
      541 gccgccaccg aggcaaacat catccacaac cagcgcgagc actgccccgc gctagcccgc
      601 gtagacttcc ttgacacgat cccgtttgcc aaacacctcg tccccggtct gccgaactac
      661 aaactcgaca cattgctggc gcatttctcc atccagcatc cggcggatcg ccacagggcc
      721 tacgctgacg tcgacgtcac cgcgcaggta ttcctccggc tcgtcaacac cgcggacgag
      781 aacgctcagc ccagcgatct cgcggccctg gtcacaatcg ccggacgcac ggccaaatgc
      841 aacattcagt gcaacatccc agtccaaagt ggactgttcg agatctagcc tcccagtagg
      901 ggagggcaca ggcagaagcg gtctgagata ggagggtgtt catgtcgatg atcaagcaac
      961 aagcggaccg cgacggggtc ccgttctggt tcgatccgct gtgcccctgg gcatggatca
     1021 cctcgcgctg gctcctggag gtcgagcagg ttcgaccggt gcgcgccgat tggcggatca
     1081 tgtccctggc ctacctgaac ctcgttcagc gcgaaggcaa gggcctcagc gaggactacc
     1141 aggagttgat ggtcaaggct tggggtccgg tgcgggtctg cgcggcagcc gctgagtacg
     1201 gtggccgcgg cgtcctcggg ccgctgtaca ccgccattgg cacccggctt cacaaccaac
     1261 gacgtcgcga cgatcccacc gtgatgaccg aagctctcgc ggcggcggac ctgcctgatt
     1321 cgctggctgc ggccgcgacg agcacggagt tcgatcaggt catcaaggac tcacacgacg
     1381 aggcgttcaa cgaggtcggc ctcgacgtcg ggaccccggt tctccgcatc ggcggcacgg
     1441 ccctgttcgg ccccgtgatc acaccggccc cccgcggtga agctgccgga caactctggg
     1501 acggcctggt catggtcgcc gcgacggacg gcttcttcga gctcaagcgg agccgggacc
     1561 gcaaaccgtc tttcgagtga gtagacacca cagcacggcc gacgccggct gaggctgcga
     1621 tcgtcgatgc tccgcgttcg gtccaacaga ggcgaacacg gatcgcgcag cctcggccgt
     1681 gcgaactgtg agccggggct gctgtcgacc ggaagggctt aatgggtccg gtcagccgat
     1741 ccgggccggc gcgctgaccg ctcgtgcaat gcggttcccg actaggcgct cggccgcggt
     1801 gagcgggtac caggcggcac cggtcacctc ggactcctgg atgcgcccca cgtccgcatg
     1861 tgccgtcgtg aaggagtatc cgatgtcgag gtggtagtgc tccggctcgt ccttctccgg
     1921 tcgtgccgga acggggccgt actcgatgta gacgggagtc tgcaaggctg ggaagaccct
     1981 gtcggggtca acacccgtct cctccgtcaa ctcgcgcgct gccgcgtcga tcagtgcggt
     2041 atcggtcggc tcaaggtggc ctccaggctg caacgtgatc ccgtacgcgc ggtgctcgac
     2101 aagcaggatc tcagcgtcac cgcggacaag cagcgcaccg acagtcacgt gcatcgggaa
     2161 gctccgccgc gaggcgaaat ccccaccttg agacagcaaa cgcgccggct ccgagagcag
     2221 cgcggcctcc tccggatagc actcgaggta ggcggcaagc acgctcgaga tgtcgccgtc
     2281 actgatcgcc acggggcacc cccacaccag tccgagagca aagctctccg agccatcggg
     2341 cacggatgtg aaagacgaga accggcacag tcagactcca tagaaggcgc gcggcgtgcg
     2401 agaaggcgca gcctacaaga cgggccttat cggtgcacaa ttgccacgct gagcggctca
     2461 cgtcgacggc gaggtcgggt ctgcgggggc cgtcgcagcg atcgccgggc ggaccgcttt
     2521 ctggtggcta tgttccggag gtcgtgccgg gattggtatt gatgccgaag cctccgcgtt
     2581 gtacggggcc accggagacc gggatgctgc cctttcgacc gatcaccggg gccggttcac
     2641 caggagccgc gaccggtgtc gtgaaggtgc ctccgccagt gatccggtct ccgaccttcg
     2701 gtgcggtctg gccgttggcg cggtagtaat accagaaccg ctgcggtgac gggtgcacat
     2761 ccaacaccgg tgtgctgccg gtgtattgcg cgggtgcagt gtcacagtcg gagtcgtcca
     2821 agcgtttctc gctccgaggg tcgacgcaga tcgatcggaa gtcatactcg cgcggctgtg
     2881 gtggcgtgac cggcggagcc ggcgcactcc ttgacggtgg tggcagtggc ggcacggccg
     2941 gtagggacgg ttgcgcgggc atgcaggccg aggccccgta cagcccggca accagcggaa
     3001 cgattttcaa cgcgggtaac gtccctctca gtctcattcc catcagttcg ttcgttcgct
     3061 ttctcttcgc agtgttggcc gggtagccaa gtaagaagaa ggcggcacgc tggcctggtc
     3121 gttgcggtcg ccaatgacgt aaccgaccac gacggggcac cggaccggat ggccttgtgc
     3181 tgtgaacgcg gcagggagat acggggtctc ggccttggtg atcgaaccag ctcgcccgcg
     3241 tcggccgggt gccgcgtgat gccggacccc ggaggtaccg acatcgagcg gccatcaggc
     3301 ggggagccgt ctcgccgaca ggacctggcg cgacgagacg actcgaccct cacaggcgtg
     3361 cgccagtgat gcgggatccg cacctcagcg cttttgtaaa agcgccccga taggagcaat
     3421 gagctgtgcc gcgtcgtagg tggtggcgga gaacgctcgc gcgaaggagt agaactcccc
     3481 catgttcatt cgcacctgcg cgaccagggc gagtatgccc acgcacagaa ccattacgaa
     3541 gatcacggct ccgatggctt tctcaagcat gcgacctgtt ttcggttaga gagccagcgc
     3601 ggagcactgc gctccgcgct ggcgcgcatc aactactgaa atcgaagggg tagcgccccc
     3661 tagcggtcgt ttctacgatc cacgcgaagc aggcgggcgc gtcggtagcg atgaagaata
     3721 aaagtagccc ctcatcgatt ggctcatttt gaaataggca atgttcccct gcctgctggt
     3781 gcaggtcgat ttgatgaccg agtcagcgtc agctcgacgt gctctaatcc ggactcgtag
     3841 gcgaacacgg aaaaataatg tcgattcttg aggttcagtt tgtttctgat ctcttttagg
     3901 cgcgatttga cgctatcctc ttcaatgtat tcgagttttg cgattgcatg gttggtcagc
     3961 ccttgggtga caagtttgat tgttcttatt tcccgatcat gcaagaggtt tacctgacct
     4021 ggatcaaaag gggtgtcaat caagttgaag ggggcgtcga aaactgcgcg catgtacgtt
     4081 acgttgacct tttggtgtgc ccaactgggg aatgcgaacc cagtctcgta cagtgccgcg
     4141 acgaggtggg tgcggtcccg tgccctaagg tcgcggcgga ttctgcaaag atgggtcttg
     4201 actgtgtacg cgcttaaata tgtgtctctt gcggtctcgt cgatgttgta gtcacgaagg
     4261 aggctcaggg cgatacggcg tcgcgcggga gtcagacgag aaagctggct cgggccccat
     4321 tcatcgggct tccatgaggg gcgtttgtgg atatgcattc cggtcatttg ttctgatgtt
     4381 gtcgttcctg ttgtggggct gatcagtggc agcggcgggc cagccgctcg gggtcgagga
     4441 tgagcacgct cttaccctcg agccgcagcc aaccgcggcg cgcgaagtcc gctagcgcct
     4501 tgttcacggt ctcccgcgag gcgccgacgt actgcgcgtt ttctttcccc gcagcgcggc
     4561 caaccgctgg tggagcagct catcaagttc cgggatggtg cgccgctcca acagcacgtc
     4621 ccagtgagtg cgcggtggct tggccttctt ctgctccggc tcgctgccgt cgatgcgctt
     4681 cgacgcggtg ccgtgcatgc gacactccca ggtgggcggc acgtcggcgt cgccggagaa
     4741 cggcaccgcg aacttgtggc tctggggaca ggagtactgg accgtccgac atggggccgc
     4801 accctgatcg ccaggtctgg aaagactggt gtggtagcca cgattcccgg tcacagccgt
     4861 cacgcgacct cccccagttc gtgcagcgtg gtgcactcca cgaccgacgt atcgctagac
     4921 tttcggcacg tccccggcag atagctgctg ccggggacgt tctctgtgcc ggtcctggtt
     4981 ggcggcccgg tcatcgagag ttcaagtgcc ttgatgtgcc gcaccaggtt cggtatccgc
     5041 agcgtcgaca ccgtctcgat ggcgtgacgc cccgacaccg attcctccgc gtacggaagt
     5101 agagcgcgcg gttcgacaag atcatcgacc catgtcggag cgctggccaa acacgctaga
     5161 tgcggctggt agcgcggcca gcgggccgct gggagatacc cggtgcaggt ctccagttca
     5221 tctgccagtt tgtggccgca cgccaggtcc ccagtcccgg ggaggcggca gtgcaccacg
     5281 gactcactcg ggctcaccac ccactccact gtctcgatct cggctgcctc ctcggagaac
     5341 gaccgaggca gggacggtag caggcggttc attcttttcc ctccgcaaac agatgctggg
     5401 tttccgtttc agctgggctg gtgatcaacc accacaaacc cgctcccaaa gctccggcaa
     5461 ggcccaccac gaccaacaag gtgaacgcca gatcgttcac gcccccaccg ccttgcgtcg
     5521 tgcgctctga acggcagcga tctggacgcc aatcgcgacg tacaacgcct cagcttgagt
     5581 ggtgtccagc agccgctctt cacctgccac acgtagccac acggtcgacg gggtatgccc
     5641 gaccacgacg tcacgatcct ggccagtgcg atcacggcac gtcacggctc tcagctcgtt
     5701 caacatgctt tcctctcctt gggaaacggc cgggatgcca gggacgggga caccggcatc
     5761 ccggccgcgt tcggggatct ccccgcgtcg cggaagaacg acgcgggtgg ctgcctactc
     5821 aggcgatcga tgctgaaacc acggcatgaa ccgtgacgac acgtctcggt ggaaagcagc
     5881 ggcggcttca gcgacagtga tcgtgccgcg cacattcacc tccgcatcga ccgcggggca
     5941 ccgcaccgac tccggcacac cgacctgcgc agccaggtgc acgacgacat gcggagcgaa
     6001 ctcgacgatt agctcggcca gccggttggt tcggatgtcg cactcctcga cacgacagga
     6061 caccggagag cgccaccacg gccggcggcg atccaccgac accacctgcg cccccggccg
     6121 gcacagttcc tcgaccaccc gggatccaat gaaccctgat ccaccggtca ccaggacacg
     6181 gcgaccaccg agttgcctat ccagcgcggg aagctgtggc ggtgacatcg tggacctcct
     6241 tgtttcgatg aatccgcggg ataccctgcg ggtgtggatg tgaacccggc ccgttcgcgg
     6301 cgagaagagc tgccaattcc cttcgcacct gccgtggttg caaccgaggg accggcctcc
     6361 cgccctgtga ggagaccgca cccgtcctcg ccggcggcag gggtgctcac atcaggtgca
     6421 aaccggtttg catgcggtgc aaaacctctg gccactcccc gttcacgggg ctagggttgg
     6481 tcggcggcgc gatctcggct acgagcggaa acgaatgggt gcgggcagaa cctcatgggc
     6541 ggcgagcgag gctccgggcg agctgatccg ccgcaagcgc aaggaaaagg gtctaacgct
     6601 agtccagctc ggtaaactgg ccgggttttc agccgcgcag gtctcccgct atgaacgtgg
     6661 tgtgtccgcg ttgacggacg tggcggtgct gcggagcttc gccgacgctc tgggaatcgc
     6721 accacaggcg ctcggactca catccggtcc ggccgggaac ggacacgcga tcgcaccggc
     6781 cgactatccg cgccttccca cgtctaaggt gcaggagaga tccggacgag aggacggtga
     6841 ggacccggtg cggagacggc agttgctggc gaacctcgca gtcaccgccg caaccgccgc
     6901 aggcgccccg ctcctactaa caaccaccgg cgggccggta agcgaagcgg cactgggccc
     6961 cgtgctcatt acgcggctac gcgacgccat gctcggcctg caccccggca cggataaccc
     7021 gctcccgccc gaggtggatc tcacacgggc ctacaccgat ttccgcgcat gccgctacgg
     7081 cagcctcgcc acccgcctac cccgtctcct gcacgcgggc catgcccatg ctggcggtga
     7141 tggcaatacg ctgctcgcgc agacctacct gctagtggtg cgcgtgctca tcaaacttga
     7201 cgaccagcaa ctggggtgga tggccgccga ccgtgcccgg cagtacgccg aggccaccgg
     7261 cgacgcactc ttcgtcgccg aagccgcccg ccatctcgct gtcctggcac gcaaagcccg
     7321 ctggtacgag caagcgatga acatcgccct gaccgcagcc gagcacccag aactgcgacc
     7381 cggcggaccg gaacacgccg ccggacgcgg cttgctcatc cagtcggcgg cgtacaccgc
     7441 cgcgaaatgc ggcgacgcag caggcatgcg ggaactgacc accgaagccg ccaccattgc
     7501 cgacggtctc ggcagaacgc tcctacctga tcacggcggc ttcagcacga ccacggtgcg
     7561 gctgcacctg atctcagcgg aactatccgc cggcgacccc gccgccgcac ttacagccgc
     7621 acgcatggtg gccccgcaga cactgcccag cactgagcgc cgcgcccgct actacaccga
     7681 cgtcgccacc gccttcgctc agtggggacg gcgtgaacag tgcctgcgtg cactcctgac
     7741 cgccgaatcc cacgccccgg aagagaccca cctgagacca gcagtcaaat ccctggtcac
     7801 cggcttgctg gtctccggac acaccaccgc cgagctgcgc gggctagccg cccgcgtcgg
     7861 ggtgcccgca taacacggga gtcggcacca acacacatgc gataattgcc gagctcaagt
     7921 cggaggtctc gggtcaccac aactttcggt acgtgggctt cgacctgcct gtggggttcc
     7981 agaagtcggc tggaggcgct gatcagcagc tttacgctgc acgctcgtac tcgttgatca
     8041 ggccatccag cacaggtcga gtcttgatcc gcttatggct gaggttcgcg actgggtggc
     8101 ttgatcgtgg tgggcggagt tcgcgggcgc ggtgtggtcg gcggccgttg tagtgccgga
     8161 cgtactctgc gagcaccgca cgtaggtgtc gttctccgaa gatcaacatg cggtcggtga
     8221 gttcggctcg gacagtgcgc acgaaccgtt cggcaaagca gttcgcccgc aggcagtgcg
     8281 ggggagtctt ggctacgcgg atgtccacat cggcgaggat cgcatcgaaa gacgcggtga
     8341 actggccggc ccggtcccgt atgaggaatc ggaattcggt gacgtcatcg ccgaggtcca
     8401 tcagcaggtt gcggacctgg gacaacgaga tcaccagatc gtccaccccg gacaggcggc
     8461 gctgccgctt cttcacgatc gccggctcga aactcccgtc gcgatcccgc ggcacgctga
     8521 tttccaccgg aaccgccctc ggtcagcacc gtcttggccc gtgtgcgtta cgcgagttgc
     8581 cgccgtcgcg gccgaccggg tcgtgcttgc cgtagccgag atgatcattc catctcgccg
     8641 gctagcgcgg cctcggtgac cagcttggtc agccggccca gcagcccacc ctcaccagtc
     8701 agccgcagcc cttcagcacg ggcccgatcg gccagctgct ggatctgctc atccattacc
     8761 gccaggtctg ccgcggcggc ctcgctggcc tgctcggcct tcacctcagt cactgccgcc
     8821 tacatctcag gagttacacc gttaaaatta cagtcccgcc ggccggattt tggaccttgc
     8881 tgtgggagag tgtgctggac cgtgcctgac caggtcgttc tcttcgatgg ggttggtaca
     8941 cgatggctca ctcgtagggg tgcgcggtca taaagcatgg gtttcgcatg ggcacgaggc
     9001 tcggttcgac gtcgccttcg aggtgccacc gcgcagcatg accttgcacg gatgtggttt
     9061 tcggcaggtg cagcggtgca ggcatgagaa cagtctcgcc ggcagccgtc gccatcaatt
     9121 tcaggtgtcc cgtccaagag cgccgccttt cttggtgcgg gctgcgggac gagctctcag
     9181 tcctgcgctg gtgagatgtc ggcgaatgcg gtcccgagtt ggcgcatggc ggtgtcgagt
     9241 agttcggtga gctccgattc gtcttcgttg agccattgtt cgtaggcggc gagggcgaca
     9301 ccgagggtgg cgtgggcgat cgtctgcggg gcaagggcat tgtcgggttg gccgaggcgt
     9361 gtgccgacgt actcggcgat cacttgcctc caagcccgga accgcaacgt ggagtgcgcc
     9421 tgcagagccg gcacgccgag gatgagttgc atgcggcgcc ggtgcgccga gatctgctcg
     9481 ggagcgacct ggttgaagtt gaccaggcag tggcggatcg cgtccatcaa cggggtgttg
     9541 gcggacaggg cgcggaagtc ctcacgcatg cgggcgagct catggtcgaa gtcgccccac
     9601 gggatgtcgt tcttcgaggg gaagtagtgg aagaacgtcc ggcgcgagat gccggcggcc
     9661 tgggcgatgt ccgtgatggt ggtgctgtcg aagccctgct cgtcgaacag ctcgaacgcg
     9721 atgtgttcga gctccgccgc ggtggtcacc ttccggcgcc caacctggcg tgtgttcttg
     9781 tcagcagtgt tgtgcgccaa cgggattcgc tccatgcctt gataggtgca ctcggtgcat
     9841 atactctcac tgtcacgccg acggccgtcg gcccccgcga taaggagcat cgcatggctg
     9901 atgaaatgcg cgaagcgagc actgtgtccg ccgaaccggc gatcgaggaa gaacttctgg
     9961 ttgaggaggt ttcgatcgac gggatgtgcg gggtctactg atgactgccg cggtggagtt
    10021 cgacacgagt ctgggctacc ggttgaatcc gcaggtcgcg ttgcgtcctg agccgttcgg
    10081 ggcgcttgcg tatcacttcg ggaaccggaa gttgtcgttt ctgaaagtcc cggagctggt
    10141 ggacttggtg cgcggtctgg ccgaccacgc cagcgtcgag gaggcattgc aggaagtctc
    10201 ggagggccgg cgggcgcagt tccggcgtgc gttggcctcg ctggcggcta cggacatgat
    10261 ccggccccgg tgatccggag agatgtgcat gaagtggagt gaatggtgac tgctgtttgc
    10321 gaacccgcac ctgttccggc gttggtcgag cagttcaagg gcgggctgga tgccccgatt
    10381 tgcctgacgt gggagtggac ctatgcctgc aatctggcgt gtgagcactg cctgtcgtcg
    10441 tcgggtaggc gggatccccg ggagctgagc accgccgaga tgaaggctgt gatcgatgag
    10501 ttgcagcgga tgcaggtctt ctacgtcaac gtcggcggtg gcgagccgac ggtgcgtccg
    10561 gacttctggg agctgctgga ctacgcggtc gagcaccagg tgggcgtgaa gttttccacc
    10621 aacgggttgc ggatcgatcg tgagcgggcg cggcagctgg cggccaccga ttacgtggac
    10681 gtgcagatct cgctggatgg cgcgacgcgc gaggtcaatg acgcggtgcg gggtgagggt
    10741 tcgttcgaca tggcgatgcg cgcgttggag aacctgtccg aggcgggtat gcgggatttc
    10801 aagatctcgg tggtgatgac gcggcagaac gtctcccagc tcgacgagtt caaggtcatc
    10861 gccgatcggt tcggcgcgca gttgcgcatc acgcggttgc gtccgtcggg tcggggtgcg
    10921 gatgtgtggg acgagctgca tcccaccgat gcgcagcagc atgagatcta ctcgtggttg
    10981 gtggagcacg gtgatcaggt gctcaccggt gactccttct ttcacctcaa cgctttgggt
    11041 tcgacgccgc tgccggggtt gaatctgtgc ggggcgggtc gtgtggtgtg cctgatcgat
    11101 ccggtgggcg atgtgtatgc gtgcccgttc gcgatccacg atgcgttcaa ggcgggcaac
    11161 gtccgcgaac cgggtgggtt cggctcggtg tggcgggagt cgacgctgtt cgaggacctg
    11221 cgtcggccgc agtccggcgg cgcgtgccag tcgtgctcgg cgttcgacgc gtgccagggc
    11281 gggtgcatgg cggcgaagtt cttcaccggc ctgccgctgg atggcccgga tccggagtgt
    11341 gtgaagggac atggccagaa ggcactgtcc gttgtagacc gttccggctt gccgaaagcc
    11401 tcgcagaacc actcccgcgc ctctcggcgc aaggtgagct tgggcatgcc cggcgtcccc
    11461 gccaaaccct gcgactccag ccccctggcg agctgaccta agcaagagga aggaagccgt
    11521 cgatggcatc cactagacag tggttcgaaa ccgttgccga agccagggag cgggcgcgca
    11581 agcggctgcc gaagtcggtg tatttggctc tgttggcggg ttcggaacgt cgaaccacgc
    11641 tcgacgacaa cgtagaggca tttgccgagc tagggtttcg cccccgaatc gcggatctcc
    11701 cggccatgcg gagccagact atcgaggttt tggggcagga gttgtcgttg ccggtgatct
    11761 tgtcgccgac cggggtgcag gccgtgcatc ccgacggtga gatcgccgtc gccaaggcgg
    11821 ctgcggcttc cggcactgcc gtggggctgt cttcctttgc cagcaaaccg gtcgaggatg
    11881 tggtcgcggc caatccgaag acgttcttcc aggcctactg gctcgggagc cgggagtcga
    11941 tccagcagcg gctggaccgg gccaagcgcg cgggcgcgaa aggcctcatc gtgacgctgg
    12001 actggacatt cgccaccgcc cgggactggg gaagcccgcc gctgccggag cgggtcgaca
    12061 tcaagacgat gctgaagttc gcacctcagg tcctggcccg gcccggttgg ttggtgcagt
    12121 acctgcgcag cggcagtctg cctgatctga ccaccccgaa tctggttgat ccggaggaga
    12181 aggggccgac gttcttcggc gcctatgggg aatggatgca gacagcgcct ccgagttggg
    12241 aggatctggc gtggctccgg gagcagtggg acgggccgtt cctgctcaag gggatcacgc
    12301 accccgatga cgcgcgccgc gcggtggaca tcggcgcgac cgcgatctcg gtgtccaacc
    12361 acggcggcaa caacctcgac agcaccccgg cgaccatccg ggtgttgccc agcatcgtcg
    12421 acaccgtcgg cgaccagatc gaggtgctgc tcgacggcgg tattcggcgg ggcagtgatg
    12481 tcgtcaaggc actcgcgatg ggcgcccgcg cggtgatgat cggccgtgct tacctctggg
    12541 gcatggcggc cgggggcgag cgcggggtgc acaacgtcat cgagatcctg cgcagcggca
    12601 tcgactccgc gctgctcggg cttgccaagt cctcggtgca cgaggtggat cgcggtgatc
    12661 tcgtcatccc cgacggattc acccgtggga tgcggtgaga catgagggaa ccacgagtcg
    12721 ctgttgtcac cggcgccgca aaggggaatc ggtgcggcca ccgttcgccg gttggcgtcg
    12781 gcggggtggc gtgtggtggc tgttgaccgt tgctccgatg acaagcgggt tccctaccac
    12841 ctggccacgc gggaggagtt ggctgctctc gtcggggagt ttcccggggc ggtcacggat
    12901 gtcgtggccg acgtgcagga ccgagccgct ctcgcggacg ctgttgcggt cgccgagcac
    12961 gagttcggtg ggttggacgc ggcggtcgcg tgcgcggcgg tcatggccgg cgggcaaccg
    13021 ttgtgggaag ccgacgacga cgagtggcag gtcttgttct cggtctgcgt cgacggggtg
    13081 gccaacctcg cgcgcgttgc ggtgccgagt atgttgcggc acccggagcc ccgcacgggc
    13141 aggttcgtcg cgctcgcttc agcggccggg catcgcggct tgttgaacct ggctgggtac
    13201 aacgcggcca aacacgccgt ggtcggcctc atcaagggct tggcccacga cctacgcggt
    13261 actgggatcg gcgccaccgc ggtctccccg ggctccactc ggaccgcgat gctggacgcg
    13321 accgcgcagg tctacgggtt ggagtccacc gaggaactcg cgcagcacca gttggttgga
    13381 cgattgctgg agccggacga ggtcgccgct gccatctgct ggctgtgctc agcggactct
    13441 tccgcggtga gcggtagcgt catccacgcc gacggaggtt tcaccgcgtg agcgcttccg
    13501 gttctccgac aggcactccg gtgccgctgg tcccgcagtc caccggctca cccaggcacc
    13561 gacgatgaac cttgccacga agcactggcc tcacctggct gccggccgcg gcgagcacct
    13621 gctggcggtt ccgctgggtg caacagaaca gcacggccca catctgcccc tgggcaccga
    13681 caccaccatt gccgaagaac tgtgccgaag acttgcccag cgcatgccaa aaatccttgt
    13741 ggctccggcc atcccgtacg gttccagcgg cgaacacgcc gggtttccgg gtaccttgtc
    13801 gatcggtcag gaggcaatgc cgcttagctt gagttgatct tgcccttggt ggcggggatg
    13861 atctcgggtg tggtggatat ggcgtggtgg ctggtgcccg gttgacggat ctggtttcgc
    13921 tgggggtgct ggcctcgtcg gttcctcggg atgtggtcga tgaagtgatc gtcgcggcgg
    13981 ggcggcaggc caagcgctcg gatgggaagc ttccgccgca cgtcatggtg tatttcgtga
    14041 tggcgatggc gttgttcgcc tcggaggatt atgaggaagt cgcggcccgg ctgaccgata
    14101 cgctggcggc gtggggttgc tgggatcagc agtggagtct gcctacttcg ggtgggatca
    14161 ctcaggctcg tcagcgtttg ggggctgagc cgcttgcgga gttgttcgcc cgggtcgcgg
    14221 tcccggtggc cggggagctg acccggggtg cgtttctcgg gccgtggcgg ttgatggcca
    14281 tcgacgggtt cgagtgggat gcaccggaca ccaaggccaa cggcgcggag ttcggctacg
    14341 gcggctcggg ggagagccgg tcggcgtttc cgaaggtccg tgtggtgtcg gtcagcgagt
    14401 gcggctcgca tgcgatggtg gacgctgaaa tcggtggggt caccggcaag ggcagcgggg
    14461 aacagaccct ggcgagacgg ttgtaccggc gcctgcagcc tggctggttg ctgatcgccg
    14521 accgcaactt ctacacctgg caggactggt gcaccgcggc tgacacgggt gcggagctat
    14581 tgtggcgcgt caagtccaac acgaggctgc cggtgctggg atcgctgtat gacggctcct
    14641 accgctcggt gctgatcgag gcgaaaatcc ggggcagggc acggcaagcg ctcatcgacg
    14701 ccgccgcccg cggcgaggat ctccctcctg atcaggccag gattgtgcgg gtcgtggaat
    14761 acacggtgcc cgaccgcgac ggcaacggca agggcgaact gatccggctg atcaccacta
    14821 tcaccgaccc caccgcagcg ccggcggcgg cgttggccga gagctaccac caccgctggg
    14881 aacacgaaac cgccaacggc cagatcaaaa ccgtcctgcg cggaccggga agagtgctgc
    14941 ggtcgaaaag ccccgacatg gtccgccagg agctctacgg ctacctgctc acccactacg
    15001 cgatcagcgc gctgatctgc cgggcggcca ccgaagccga catcgacccc gaccgggtca
    15061 aattcacccg caccgtccgc atcgtccgcc gccggatcgc cgatccggcg gctttttccc
    15121 cctgaacacc agaaacgatc tctggccgct atcacctccg acatcaccgc cgcccgcaac
    15181 ctcaatcccc gccgccgcca ccgcagctac ccccgcgtga tcaaacgcgg ccgccacaac
    15241 gcctaccgag tcaaaaaacc caccgacacc ggcacccgcc acgacagccc gcccagcatc
    15301 caactcgccc ccacagcatc atgatcaaaa taggctaagc ggcattgtgg tcaggaggcg
    15361 gtcgaactcg tgctcaccga gctggtgcgc tccgccgacg ctttcgcggg cgtcatcctc
    15421 gtcaacgccc acggcggcaa tcttcaaccg ctccaggctg tgttgcgcac cctccgctct
    15481 gaagggcgcc gcatctacgc ctggatgcct agcggagcca acaccgactc gcacgcgggt
    15541 cacaccgaaa cgtccgtcat gctccacctc cagcctgatc acgtcgaaac agccttgatg
    15601 cgaaagggaa acacgacgcc gcttcccgaa ttgatcgacc gcttgcgtgc cgacggagtc
    15661 gcagccgtca gccctgacgg cgtgttgggc gaccccacgc acgccagcgc ggaccaggga
    15721 gccaccatcc tcgaacgctg gaccgaacaa ctgacgaccg cggtggagaa atggagccgg
    15781 atatggcgtg atgcgtcggc agctctgtag gcattgctga cgaagggagt gtgaaccggg
    15841 ttccgagtgc caggttgagg aatcgctatg acggcccgca gttacaccga tgcggcaacc
    15901 atgtgccgcc tgtgcggtga ggcggctgcg gtgatcaccg ggatccttgc tcacgcggat
    15961 aggaggttta cggcatgagc actctgcatt tgaggggaga ctccagcctc cagaggtggg
    16021 atggcggtcg gacactggtc ggcggatccc cactccgagt ggtgcgcctc agcgaagcgg
    16081 gtgcccggcg cgtggacgga tggttaaggg gtactccggt gcctgccgac ccggctagtc
    16141 gtggcctcgc tgaacgtctc gtccgtgcag ggatggcgca tcccgtttat ggggacgggc
    16201 aactgcgacc ggtggacgtg accgtggtcg ttccggtgcg ggacgacgcc gacatggttg
    16261 ccgacctgcg tatggccgcg ggtgacgtcg ccgaggtcat cgtggtcgac gacgggtctc
    16321 aaataccgat tgatggtgcg gccctgcgga acgagaccgc tcgcgggcct gccgctgcac
    16381 gcaatgcggg ctggcgccgt gctcgaacag ccctcgtggc gttcctggac gccgacaccg
    16441 ccttggaacg cggttggctg gaacctctgc tgcgtcactt cgaagatccc acggtggtcg
    16501 cggtggctcc ccgcgtccgc agtatggatg gtgacacgat cttggctcgc tacgaatacc
    16561 tgcgatcgcc cttggacttg ggagccgtgc caggaccggt ggattccacg tggccgaatc
    16621 cggagagggg taacgcggtg actccacgcg tcgcccgagc cgggcaaggt cgtcagccct
    16681 cgcgacgtct gcccggacac gggctggccc gtctctcctg ccagcaggcc cgaagctggt
    16741 gatctcggat gccttcacct tccaaggccc ccggcagctc ctcagcccac ttgaaccgtg
    16801 caccaccaaa gtggttgggg atcaggagat tcaggtcgat cctaagtgga tagtcatcgt
    16861 ctgcggtcgg cgcgccacac aggtttgtca gcaccgcaca aaactccttg gtgctctatg
    16921 gaatcctcgc agagttcggg tcaccagcct ccagtgtggc ggctgaggtg gcttgataag
    16981 ccgcctcagc cagaccgagc acccacaggc gcgtttactg cgcccctcgt cctcagcgaa
    17041 gggctttccg acgatctgta cgcccatcgg aagcccagtg gatgtgaacc ccgtcggcac
    17101 ctgcaacgcc ggcagaccag tgatatttcg ccggaatgca catgcgggac tatgcggagg
    17161 tggctccctc cactattccg tcgtcccagc gaaagtacag atcgtctcgc cgaagggccg
    17221 gtcctggcga cgacggagcc aaaaggacat caatctggtc gaacatccgg caccagtcct
    17281 gctggatctg ggtgcgtagc cgcatcgtgt tgatgtagtc ggttgccagg acggcttcgc
    17341 cgaccgcaag catttcccgc acgtcatcgt tgaacagctc ggggtgttgc gcgtcgcgtc
    17401 tgcgcgccgt ggctgatcat cggctatcgc gacggttccc gcgcgacgat tgttcctcgg
    17461 cgcgttcgcc gcgctgctgg acctgcagcc ggacatcacg gtgatggcga ctgcgggaac
    17521 tggcgacgaa gcgctcgccg cggtcgagcg gcaccggccg cacatcctgc tcacggacat
    17581 tgaaatgcca ggccgaaccg ggctcgaagt ggcggcggaa cgcttcacta catcgcggag
    17641 atcattcccg ggggtcgcgt acacatgcag ccgacgctgc tttggccaca gtcgttgttc
    17701 gacaaagaaa ttccgcatga ccatccttcg tgatcgacta cacccgagtc ccgaatcagt
    17761 attgtaaaca ttcagctggt gcactgtgtg tagtgccagc ggtgctggcg tagcggtctg
    17821 tgactgctgg ggctgtgtgg tttctgtgtc ggcgtgtcgc tggctgatct tctggttcgg
    17881 aactgctgtg atgctggggt gcctgtgccc gaggcgatga cccgtgacga gttgatcgag
    17941 ctcgtgggtg agcagggcgc gcggatcgcg gcgctaggcg agcagatcgc ggtgcgagac
    18001 cggcagatca cagtgatggc cacgcagatg gccgagttgg cggaggtcaa cgagacgctg
    18061 ggcgagagac tggcgaagct ggagcatgtg ctgtcccgga acagcaagaa ttcgtcatct
    18121 cctccgtcca aagacgacgg cccgggcagg acaccgcctc cggcgaaggc gaaacgcggc
    18181 ggcgcgtcga agcggagggg caagcagccc gggactccgg gggcgaacct ggcctggacc
    18241 gattccccgg gagatcacaa ggaccggttt ctccggggcg tatgcgagtg cggcactgac
    18301 ctggccgggg cacgggatct gggggtggtg gatcgctacc agcagcacga gatccccccg
    18361 gtgtcggtga agatcaccca gtacgaccag catgcggtgc gctgtggctg tggcagggtg
    18421 cacaccgctg cgcgcccgga aggcgcccgt cccgggccgg tgggctacgg cccgaacctg
    18481 caggctttcg ctgtgtatct catggtcgtg cacttcattc ccgtgcaccg ctgcgtcgag
    18541 ctgttggcat cgctgaccgg cgccgcgccc agcgtgggtt tcgtgcacgg catgcttacc
    18601 cgcgctgcgg gcgtgttgac cgaggtcgac acgcggatcc gcacgctgat caccctggcc
    18661 tacgctgtct gctgcgacga aaccccgctg cgagtggggc cgcgcacacc caaaccgggc
    18721 aagaagaaag ccgagaagta tctgctggtc gcctgcaccg aactgttcac ccactacctg
    18781 ctcggtgacc gggacctcgc cacgttcaag gcgttcgtga tccaggatct tgccggctcg
    18841 gtgatcgtgc acgaccgcta ccaaaactac gactccgccg aactgggcga gctgacccac
    18901 caactgtgtt gcgctcacct gggtcgtgat ctcgatggtg ccggcgaggt ctatcccggt
    18961 gcgcactggc ccacccagat cgccgacgca ctgcgcgggc tgatccacga agccaacctc
    19021 gcccgcgcca ccggccggga cgcgatcgag cagaccatca aaaacgagct gatcaagcgg
    19081 ttccgggacg gtgttcgggt cgggctgtcc gacaccacca gccacggaaa ccggcccggt
    19141 gagcgcaaga cccggctact gctggaagtc ctgcgcgacc gcgagcccga cgtgctgcgc
    19201 ttcgcccacg acctgaaggt tccacccacc tccaaccaag ccgaacgcga cctacggccc
    19261 gccaaggtgc aacagaacat ctccgggcgg ctgaccagcg agaaacgcac caaagaccgc
    19321 taccgaatcc gcggatacct ctccaccgcc agcaagcacg gccgcaacat gatcgaagcc
    19381 ctccgcgagg ccatccgcgg acacccctgg atgccaccgg accccacccc agcctgaccc
    19441 gcgccaaccc cgaccggcac caaacccatc gggcgatcac gccatgccat cacgcagacc
    19501 actcacggca atccaactga atgtttactc agtatcacct cacggttctt ccgagacttc
    19561 ggcggctcgt cgttctccgc gctcccggag agttcttcca gaagcgcgtg agcttttccc
    19621 ataatgtcca acgcgatccg ggttttcgag aggtcggatt tcagtttctc gttttcgcgg
    19681 cgtagctttt ccagttcgag ttgctcggcg gtcttcttcg ggcgtttcgg cggtgacccc
    19741 gcatcggcgg accgggcggt gagaacaccg gcgtcacggt cgcgtgtcca ttcgatgaca
    19801 tgggaggaat acaggccttc ccgccgcagg atcgcgcctt tctccccgtt cggggcgttc
    19861 tcgtactcgg cgacgatcgc cagcttgtac tccgggctga acacccgccg cgaaggccgc
    19921 ggcgccggat cggcacccgg tggcgtcgag gagccagact ggtcatcaga cggtgtggac
    19981 acggacacaa ggatctccga tcccgtcctc tgatcagtac ccggtactca caccgggtgt
    20041 ctcacctcag cctgacaaag atgggatcac ccacgccgac ctcaccaggg acatcacggc
    20101 gcgccagtcg ccacggctgc ccggaactcc agcacgaacc ggccgtccgc agccccacac
    20161 gccgcagcag tagcactggc cacccaacca cccaagctca acgagcacga tcttgggcca
    20221 accatactgg cacagtcacc gaacgggccg cattgaatgg caacgagccg acctgcgtgt
    20281 cacgggacag ccagctggcc gctggtgccg gaggatcgtg acggcctgat cgtgcaggcg
    20341 gtcctactcg ccatcgtcgg cggcggcgcc atccgggtct tgcccagatc agacttcagt
    20401 ttcttgttct cccggcgcag tttctccaac tcgagctgta ccgcggtctt cttcgcccgc
    20461 tttggcgccg ccccgccggc cttgtcggac cgggcggtcg ggacaccggc atcacgggcg
    20521 cgtgtccact cgatgacgtg ggaagaatac aagccctccc ggcggaggat cgcgcctttc
    20581 tcgccgttcg gggcgttctc atattcggcg acgatcgcca gcttgtactc cgggctgaac
    20641 acccgccgtg agggccgtgc ggcaggatca ggctgctgag cgggctggcc gggcctgtcg
    20701 gatgaagtgg acacgtctac caggatctcc gatcccgccc tggctccggt acccggcact
    20761 cacaccaggt gtctcatctc atcctgacaa agaagggcgt gcggttgacg ccggcgtgat
    20821 tgcgatctcg gtttccaacc acggcggtaa caaccttctt ggcgagacca gctgtgtctg
    20881 agcctgtcag acgccgcagt cacaccgaac gatccacatt ccgcgctgtg acgaatgtgg
    20941 cgatttgtgc gcgggaggtg aagccgagct tgcggaggac gtgttcgacg tgcgcctcga
    21001 ctgtgcgaac actgacgatg agtgtgtcag cgatctccct gttggtcagc ccatcggcga
    21061 ccagggaggc gatctgggct tcccgcttcg tcagagcgtg gtcgacgtct tgcctcttac
    21121 cttggggtgc ggcggcacca tgggtgtggc ctaaagcgat ttcgaccgcg tcgtcgggct
    21181 ttcgctcgcc ggcggagaag gccgcggtga aggcttcgga gccgagtgag ctctcggcac
    21241 gtgcggtgca ctgcgcgctc cactcctgta gacgggtgaa tcctgccatg tgcgagccca
    21301 gcggctccca cagagcacgg ctcgcaccca ggaatttcgc ggcctttgtg gcgtcgccgt
    21361 tggcctctgc agtccaagcg agcacgttga gcgccatggc cacgccgaga gggtctccga
    21421 gcgtctgctt gtaccggaga gcgtcgatga cagtcttttc cgctttcgtc tggtctccgc
    21481 ggagccagcg cgctatcccc aggtacagct ggctccacga catcatccat tgttcgttat
    21541 gggccgcaca cgcaatccgg cattcctcga ggatctcgag ggcacgatcg acgcggtcgc
    21601 cggagatcgc cgcgatgccc aaatttagct tggcgagggt gaggtgcagg ttgaacccct
    21661 tcgcctgggc ttcaagggcg acaccttctt ggagataggc ggtgccctcg tcgacgtttg
    21721 cgcggaacag ctcggcgagg ccgcagatgt aggtcgcatg agcgtaggag agtgggtcgc
    21781 cgatttcctg agcgagattg agacactcgt ggaggaccac tagagcctgg tcgggagcgc
    21841 cttcaacggt cagaagatac cccttcaccc agagcgcagg agctcgctcc ggataagggt
    21901 ccctgtttgc cttcaggagc tggtccagcc agcgcccgcc ttcccgcggg tggccacagg
    21961 cgatccagaa ggcccagagt gcactggcaa gggcaagtcc ctcgcggtct gcactgggtt
    22021 gggagaggga ggactcgagc gcaactcgaa ggttcgcgtg gcgccgctgc atatcttcga
    22081 acagcctgat ctcctccgga ccgaaccagg ccgactcgaa gtaacgggcg acggagaggt
    22141 agtgatcgcg atggcgtgct acgacctcct gctccatatt gatttcggcg agtcgttccg
    22201 cgccgtagtg gcgaatggtt tccagcattc ggtagtcggt gccgtcgtag ctgacgatgg
    22261 acttgtccac gagccccacc aatgcgttta ccacgttccc gtggtcggcg ctgacgggtt
    22321 cgcacactgc ctcgattgcc gccagctcaa tcggtccaga gaagaccgac aaatcggccc
    22381 agagccggcg ctcgtcttcg gtgcagaggt cgtagctcca gtccatcgcg gcccgcaccg
    22441 ttcgatgtcg atcggctact gccctatcgt cctggggcag gatgtcgtag ctggcgcgat
    22501 gtcgcatgag tagttcgtgc accgacatcg agcgcatcag tacactcgcg agttcgatcg
    22561 ccagtggcag accatcgaga cgcgtgcata gctccacgac ctcagaccga ttggcgtcgg
    22621 aaatagcgaa atgcggggcg gcctcgatcg ctcgctggac gaagaggttg accgcatcga
    22681 aacggatctc gtcgccag
//
