LOCUS       NZ_CP040605            10990 bp    DNA     circular CON 25-AUG-2024
DEFINITION  Saccharopolyspora sp. ASAGF58 chromosome, complete genome.
ACCESSION   NZ_CP040605
VERSION     NZ_CP040605.1
KEYWORDS    RefSeq.
SOURCE      Saccharopolyspora sp. ASAGF58
  ORGANISM  Saccharopolyspora sp. ASAGF58
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 8045912)
  AUTHORS   Guo,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2019) Grain Processing Research Group, Academy of
            National Food and Strategic Reserves Administration, Baiwanzhuang,
            Beijing 100037, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.15x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012277335.1-RS_2024_08_25
            Annotation Date                   :: 08/25/2024 04:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,606
            CDSs (total)                      :: 7,535
            Genes (coding)                    :: 7,265
            CDSs (with protein)               :: 7,265
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 228 of 270
            Pseudo Genes (internal stop)      :: 14 of 270
            Pseudo Genes (multiple problems)  :: 48 of 270
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:54:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5403343
            Orig. end                         :: 5414333
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040605.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5990)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            247..1290
                     /locus_tag="FDZ84_RS25485"
                     /old_locus_tag="FDZ84_25530"
     CDS             247..1290
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.5"
                     /locus_tag="FDZ84_RS25485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25530"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_168587931.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 7.3e-12, bitscore:
                     45.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVKRFVAALAVALVAVGLPVLAPPEASAQAIVDFPVSFQVQNVN
                     RTTSQCTADGHTYTIRGHLTAPASVLSRPNPAVTLYVHGTNTGEWIWRLDSPGHDYVRT
                     LAEKGHASVTIDRLGYGSSPIPNGFATCSGAHADIADQIVRQLRAGTYTIDGAAPARFT
                     RVFMAGHSSGALVAETAAFSFGNVDGIVLTGWAAIGLTEDTMRRFFSAYDRCLEGGEAG
                     YPPGFVHFDGSLDGFLAGGLGPDAEPRVREAITPLYPANPCGVMVSEPVGIMSDLTRIG
                     EIKVPVHLIFGAKDVLRQGVESYPGLFTSSDDVTATTVPDAGHFVTLDVNSSVVTDGIA
                     DWLDRHQ"
     gene            1311..3590
                     /locus_tag="FDZ84_RS25490"
                     /old_locus_tag="FDZ84_25535"
     CDS             1311..3590
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418458.1"
                     /locus_tag="FDZ84_RS25490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25535"
                     /product="MMPL family transporter"
                     /protein_id="WP_246868413.1"
                     /transl_table=11
                     /translation="MSTSPPQDQDQDPGSGTTRPPALRRLGRYIAHRPRVIALVGVVLF
                     IISLVIGSGALNRLMLSRFESPGSQSLQVAEQLQQQFDTGKPQFMLLVTAKQGTVDDPQ
                     PAAAGRALEQELANHVGVLDVSSYWSRGGSPALRSNDSRQALIIGRLEGDVTQARTELG
                     RSISPDFTREDALVRVQVGGADEVFRQAAQQARTDFFRGELIALPMALILLFAIYRRFR
                     VAALTLGMGFFAVVATLALLRGVTYVTEVSTFAANLTLVMGLGLGIDYSLFIINRYRES
                     LRAGRTVQQSVEDAVATAGKTVAFSGVTVAVSLACLMLFPFPFLRSFAYSGVGVVATSV
                     FAALVILPAVLALLGRAADRRKPPRNTSGGAWHTMAVRMMKRPLVYGVPALVVVLALGS
                     PVLGLQFGLPDDRILPPETSSRVTQQSIRDNFGQEEMDAIQLLTDDVPGTVASSAEIER
                     YATALSNVPGVAQVDALTGSYRQGQQILEPNESSQRFADEQGTWFSAVPTNEVLAGGDV
                     ATLVSQLRSAMSGVDVGVGGYPSELNDFRTALVERLPVVLVLIFVVTFIILFLMTGSVL
                     LPAKATVLNVLSMSVMFGALVWVFQDGNLAGLLDFTPTGQVEPTFPILMFCIAYGLSMD
                     YEVFMMSRIKEEHDRTGDNVQSVAVGIERSGPLITAAAVILALAFASYGVSGVMYLKML
                     GIGMALVILVDATLIRAVLVPVFMRLAGNANWWAPAPLRKLHERFGITEQETSRSEPRV
                     PSGAAH"
     gene            complement(3668..4372)
                     /gene="fabG"
                     /locus_tag="FDZ84_RS25495"
                     /old_locus_tag="FDZ84_25540"
     CDS             complement(3668..4372)
                     /EC_number="1.1.1.100"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (3-154). E-value: 6.1e-20. Score:
                     63.8. Matches aSDomain:
                     nrpspksdomains_FDZ84_RS25495_PKS_KR.1"
                     /codon_start=1
                     /gene="fabG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /locus_tag="FDZ84_RS25495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25540"
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="WP_168587932.1"
                     /sec_met_domain="adh_short (E-value: 9.9e-36, bitscore:
                     122.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRSVLVTGGNRGIGREIAQSFAKAGHRVAITYRSEPPPEDFLAV
                     RADITAPDDVEQLFRRIEEEHGPVEVLVANAGITRDKLLPRLSDDDFSDVLETNLTGSF
                     RVARRAARKMFSARWGRIIFVSSIVGFTGAPGQINYSASKSGLTGLTRSLAWELGSRGI
                     TANLVAPGLIETEMTAELTTSRREEVLRMTAMRRTGSPDEVAGVVEFLASDAASFITGA
                     AIPVSGGFGMGY"
     CDS_motif       complement(3887..3937)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FDZ84_RS25495_0004"
                     /evalue="1.80E+00"
                     /label="PKSI-KR_m1"
                     /locus_tag="FDZ84_RS25495"
                     /protein_end="162"
                     /protein_start="145"
                     /score="1.3"
                     /tool="antismash"
                     /translation="GLTGLTRSLAWELGSRG"
     aSDomain        complement(3911..4363)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FDZ84_RS25495_PKS_KR.1"
                     /evalue="6.10E-20"
                     /label="FDZ84_RS25495_PKS_KR.1"
                     /locus_tag="FDZ84_RS25495"
                     /protein_end="154"
                     /protein_start="3"
                     /score="63.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="SVLVTGGNRGIGREIAQSFAKAGHRVAITYRSEPPPEDFLAVRAD
                     ITAPDDVEQLFRRIEEEHGPVEVLVANAGITRDKLLPRLSDDDFSDVLETNLTGSFRVA
                     RRAARKMFSARWGRIIFVSSIVGFTGAPGQINYSASKSGLTGLTRSL"
     CDS_motif       complement(3947..4006)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FDZ84_RS25495_0003"
                     /evalue="1.80E-06"
                     /label="PKSI-KR_m4"
                     /locus_tag="FDZ84_RS25495"
                     /protein_end="142"
                     /protein_start="122"
                     /score="20.5"
                     /tool="antismash"
                     /translation="IFVSSIVGFTGAPGQINYSA"
     CDS_motif       complement(3995..4045)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FDZ84_RS25495_0002"
                     /evalue="2.30E+01"
                     /label="PKSI-KR_m1"
                     /locus_tag="FDZ84_RS25495"
                     /protein_end="126"
                     /protein_start="109"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="AARKMFSARWGRIIFVS"
     CDS_motif       complement(4289..4363)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FDZ84_RS25495_0001"
                     /evalue="6.10E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="FDZ84_RS25495"
                     /protein_end="28"
                     /protein_start="3"
                     /score="22.1"
                     /tool="antismash"
                     /translation="SVLVTGGNRGIGREIAQSFAKAGHR"
     gene            complement(4369..5004)
                     /locus_tag="FDZ84_RS25500"
                     /old_locus_tag="FDZ84_25545"
     CDS             complement(4369..5004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012905.5"
                     /locus_tag="FDZ84_RS25500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25545"
                     /product="HAD family phosphatase"
                     /protein_id="WP_168587933.1"
                     /sec_met_domain="HAD_2 (E-value: 5.9e-07, bitscore: 28.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFRAVWTDFGGVLTPPVNESFAGFAAQAGAPPAVLWAAMQNVAA
                     RYGVDDVMAPLDTPLTTEADWARQVTAVVREDTGAEVDLSRLGERWFAGRPANSSWVGT
                     LRKLREQGVFVGLLSNMPPSWDEQWRRMVSPELFDSVVLSYEVGSRKPEAAIFGLAVER
                     CGFPVGECVLVDDMAKNCAGARETGWQAVHFTSSDDAAAELDRQRVGT"
     gene            complement(5001..5990)
                     /locus_tag="FDZ84_RS25505"
                     /old_locus_tag="FDZ84_25550"
     CDS             complement(5001..5990)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="FDZ84_RS25505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25550"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_168587934.1"
                     /sec_met_domain="AfsA (E-value: 3.5e-14, bitscore: 51.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDAGELAPPVGAFSHTVAREMVHRWALSEVFITGFNAAGRREF
                     LVDAQLPLYHAYYTDHAARPELFDPLLLLECCRQGGTYGTHELLGLPMNSTMLVKTASI
                     RITDLDGLVIGTRPGELRMSGVFTDVEMRAGIPRNMGMDAELTLSGRSIGTARIEATAV
                     AKTTFRALRTRQRTGEPPTSSELPATPAGVPLSPHLFGRKLPENVVLTNARRDHHSTSA
                     QLGVNPRNRSLFDHEYDHYPAMSLFEAARQLSLLALDDGTGAAATRVRCTSFSAEFIRF
                     AELDSPVDLRIVGEHSNELDVHFVQDGTVIATTTVGFDQVGTSSEVAA"
     misc_feature    complement(5967..5969)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5987..6700)
                     /locus_tag="FDZ84_RS25510"
                     /old_locus_tag="FDZ84_25555"
     CDS             complement(5987..6700)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225016.1"
                     /locus_tag="FDZ84_RS25510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25555"
                     /product="response regulator transcription factor"
                     /protein_id="WP_246868414.1"
                     /transl_table=11
                     /translation="MLLHQSVARALQPFGNLEVVEDPSAISAFDGAADPRPPDVALVCS
                     TTSVNYLMANIARIRLTSPQTRILALPPMRDTELLGELRTVGVTSFGDAQTTLEELLTL
                     LDQIRIAPPPGQVTTEQIELIRARMHCHHKLSAREREIVNLVAQALSNRQIASTLRITE
                     GTVKRHMRNIFTKLGANSRIDAVNKATAGTCNCSSKQGRICSENFAKSSHCTLSGEHCG
                     TEHRPLVTAFPGGKR"
     misc_feature    complement(6667..6680)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.65"
                     /tool="antismash"
     gene            7151..8692
                     /locus_tag="FDZ84_RS25515"
                     /old_locus_tag="FDZ84_25560"
     CDS             7151..8692
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.5"
                     /locus_tag="FDZ84_RS25515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25560"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_168587936.1"
                     /transl_table=11
                     /translation="MSIKSGGTLVVGAGPTGLTVATELIRRERPVRIIDRGERPHPHSR
                     AIVLWPRGLEALRRCGAAEEIVERALPLKAGNYYTGGRRVARLVFSGGLAGTRYQAPLS
                     LPQRDTEEVLRRHFEQLGGKIEYGVSMRSLRQEAEKVHVGLGTTSGTADEEFSWVVGCD
                     GAHSGTREQLGVEFEGQAYQETFALSDGECETPLAHNEAHYFMSPHGVLVVVGLPGGLY
                     RVFVSLRPGTDQSDLNGLVQEAAQQRCPVPLRLVGEQQSGSFQVNRRVAKRFHEGRVML
                     AGDAAHVHSPAGGQGMNTGIEDAQSLGWRLASADPGTAGVAGVRAWAAERRHVAQQVVA
                     DTDRQTQMWMLKGWRRRARDGALGIAARTGLLNRVLPPRLAQLALAYPSSGTGVGMLKP
                     GVRLPDEKLGDKWLHDMLNGNALVLGFAVDRRETGNLPVVRAAADALAQQWAAQLGSEA
                     DVAIVHSGAPDMPGDLLDETGQVHRRFGITGPALVVVRPDAIVAHAERLTDDIGR"
     gene            8728..9000
                     /locus_tag="FDZ84_RS25520"
     CDS             8728..9000
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (16-81). E-value: 3.5e-11.
                     Score: 35.2. Matches aSDomain:
                     nrpspksdomains_FDZ84_RS25520_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.5"
                     /locus_tag="FDZ84_RS25520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_168587937.1"
                     /sec_met_domain="PP-binding (E-value: 3.7e-06, bitscore:
                     26.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGNENVLDGTDDVMDELKKLVTELTDLPAGALTSAASLATDLGL
                     DDLAVAQILVEAELRFGVQIPDRIVRPGLQELADYIRAEARKENT"
     misc_feature    8740..8751
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.93"
                     /tool="antismash"
     aSDomain        8776..8970
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FDZ84_RS25520_PP-binding.1"
                     /evalue="3.50E-11"
                     /label="FDZ84_RS25520_PP-binding.1"
                     /locus_tag="FDZ84_RS25520"
                     /protein_end="81"
                     /protein_start="16"
                     /score="35.2"
                     /tool="antismash"
                     /translation="ELKKLVTELTDLPAGALTSAASLATDLGLDDLAVAQILVEAELRF
                     GVQIPDRIVRPGLQELADYI"
     gene            8997..9977
                     /locus_tag="FDZ84_RS25525"
                     /old_locus_tag="FDZ84_25570"
     CDS             8997..9977
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012517.5"
                     /locus_tag="FDZ84_RS25525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25570"
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="WP_168587938.1"
                     /transl_table=11
                     /translation="MNLRLGLQLTSFSYPSTPPASLFERIVETARTAEQAGFDLFAVMD
                     HLMQLPVQGAPDEPMMEPYTLLGGVAARTARIQLGTLVTGVTYHNPARLAKTVTALDIV
                     SGGRALLGIGAAWYDEEHRRYGYSFPSVRERFAWLDEALQICRSMFEGKSTSFSGEHFS
                     VSDALNEPQPVHVPPILIGGAGEQRTIPLAAKHADVLNLNCGFPDVPRKLEVLREKCAE
                     LGRDYDAINKNVLAVVFLGRTNEEAVAKRDAVLARQRSGPAATGAGLPAALGSRMIVGD
                     ADTVGEQISGLIDLGLDGVIVNLTGDSHNPERLAHAGEVLRAALP"
     gene            complement(10141..10749)
                     /locus_tag="FDZ84_RS25530"
                     /old_locus_tag="FDZ84_25575"
     CDS             complement(10141..10749)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418457.1"
                     /locus_tag="FDZ84_RS25530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_25575"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_246868415.1"
                     /transl_table=11
                     /translation="MADQPPQRRRQARGERRIELLLDAAAEVFGEVGYAAATTNAIAAR
                     AASSPGTLYQFFPNKGEIARALSERYLRQLQQTQRKALAVEHADLPLTELIDAMVDPVI
                     DFNLSNPGFQALFNAPDEQVATKPLHVREAVIERLSAIFAARAPELPKDQRVRSARVLV
                     GVFRGVLPLIIGTKGAERAAMIGELKRICRDYLTPLIGA"
ORIGIN
        1 gaggccgtcg tggtcgacct ccagcacctg aacgagatca ccgtgccggt gctgatggtc
       61 cagggcgagc aggactcccg gatcaccggc ggtgagcaga acctcgcacc gttcaccggc
      121 accaccgaca agcagctgct gaccgtgccc ggcgccggcc acttcatggg gctgggggag
      181 cgggcgcagg tgctccacga cggcatggcg tcgtggctgg accggcactg agaaaggaaa
      241 ccgagcatgt tcgtcaaacg cttcgtcgcg gcgctggcgg tggcgctggt cgccgtcggg
      301 ctgccggtcc tggccccgcc ggaagccagt gcgcaggcca ttgtggactt tccggtctcc
      361 ttccaggtcc agaacgtcaa ccgcaccacc agccagtgca ccgccgacgg tcacacctac
      421 accatccgcg gccacctgac cgcgccggcg tcggtgctct cgcggccgaa tccggcggtc
      481 acgctctacg tgcatggcac caacaccggt gagtggatct ggcggctcga cagccccggc
      541 cacgactacg tgcggacgct ggcggagaag ggccacgcgt cggtgaccat cgaccgcctc
      601 ggctatggct ccagccccat ccccaacggg ttcgccacct gcagcggggc acacgccgac
      661 atcgccgacc agatcgtgcg gcagctgcgc gcgggcacct acaccatcga cggcgccgca
      721 ccggcccgtt tcacccgcgt gttcatggcg ggccactcca gtggcgcgct ggtcgccgag
      781 accgcagcgt tctccttcgg caacgtcgac gggatcgtgc tgaccggctg ggccgcgatc
      841 ggcttgaccg aggacaccat gcggcggttc ttctccgcct acgaccggtg cctggaaggc
      901 ggagaagccg gctacccgcc tggtttcgtc cacttcgacg gcagcctgga cggcttcctg
      961 gccggcggtc tcggaccgga cgccgaaccg cgcgtcaggg aggcgatcac tccgctatac
     1021 ccggccaacc cgtgcggggt gatggtgtcc gaaccggtgg gcatcatgtc cgacctgacc
     1081 cgcatcggcg agatcaaggt cccggtgcac ctgatcttcg gtgcgaagga tgtcctgcgc
     1141 cagggcgtcg aaagctaccc cggcctgttc accagcagcg acgacgtcac ggctacgacg
     1201 gtgcccgatg ccgggcactt cgtgaccctg gacgtcaact cgtcggtggt caccgacggg
     1261 atcgcggact ggctcgatcg ccaccagtaa acccaggaag gaagtggcac gtgtccacat
     1321 cgccacccca ggaccaagac caggatccgg gctccgggac cacccgcccc ccggcgctgc
     1381 gcaggctggg gcggtacatc gcacaccggc cgcgcgttat cgcgctggtc ggtgtcgtgc
     1441 tgttcatcat ttcgctggtg atcggctccg gtgcgctgaa ccggctcatg ctgagccggt
     1501 tcgaaagccc cggctcgcag tcgctacagg tcgcagagca gctgcagcag cagttcgaca
     1561 cgggcaaacc gcagttcatg ctgctggtca ccgccaagca gggaaccgtg gacgatccgc
     1621 aaccagccgc ggcgggccgc gcgctggagc aggagctggc caaccacgtc ggggtgctcg
     1681 acgtctcgtc gtactggtcc cggggcggtt cgcccgcgct gcgcagcaac gactcccggc
     1741 aggcgctgat catcggccgc ttggaaggcg atgtgacgca ggcgcgcacc gagctgggcc
     1801 gcagcatctc gccggacttc acccgcgagg acgcactggt cagggtgcag gtcggcggcg
     1861 ccgatgaggt cttccggcag gccgctcagc aggccaggac cgacttcttc cgcggtgagc
     1921 tgatcgcgct gccgatggcg ctgatcctgc tgttcgccat ctaccgccgg ttccgggtcg
     1981 ccgcgctgac cctcgggatg ggtttcttcg cagtggtggc gacgctggcg ctgctgcgcg
     2041 gggtcaccta cgtcaccgag gtctccacgt tcgcggcgaa cctgaccctc gtgatggggc
     2101 tgggcctggg aatcgactac agcctgttca tcatcaaccg gtaccgggag tcgctgcgcg
     2161 cgggccgtac cgtccagcag tcggtcgagg acgccgtggc cacggcgggc aagacggtgg
     2221 ccttcagcgg tgtgacggtc gccgtctcgc tggcgtgcct gatgttgttc ccgttcccct
     2281 tcctgcgttc gttcgcctac tccggtgtcg gcgtggtggc gacgtcggtg ttcgcggcac
     2341 tggtgatcct tcccgcggtg ctggcgttgc tgggccgcgc cgccgaccgc cgcaagcccc
     2401 cgcgcaacac ctcgggcggc gcctggcaca ccatggcggt gcgcatgatg aagcgcccgc
     2461 tggtctacgg cgtgcccgca ctcgtcgtgg tgctcgcact cggttcgccg gtgctgggcc
     2521 tgcagttcgg cctgcccgac gaccggatcc tgccgccgga gacctccagc cgggtcaccc
     2581 agcagtccat ccgggacaac ttcggtcagg aggagatgga tgcgatccag ctgctgaccg
     2641 acgacgtgcc ggggacggtg gccagcagcg ccgagatcga gcgctacgcc accgctctgt
     2701 ccaatgtgcc cggtgtcgcg caggtggatg cgctcactgg ttcctaccgg caggggcagc
     2761 agatcctgga gcccaacgag agctcgcagc gcttcgctga cgaacagggc acctggttct
     2821 ccgcggtgcc caccaacgag gtgctggccg gcggtgacgt cgccacgctg gtctcgcagc
     2881 tgcgctcggc gatgtccggg gtcgacgtcg gtgttggcgg ttacccgtcc gagctcaacg
     2941 acttccggac cgcgctggtc gagcggctgc cggtcgtact ggtgctcatc ttcgtcgtca
     3001 cgttcatcat cctgtttctg atgaccggca gcgtgctgct gccggcgaag gctaccgtgc
     3061 tcaacgtcct gagcatgtcg gtgatgttcg gcgcgctggt ctgggtgttc caggacggca
     3121 acctcgccgg actgctggac ttcaccccga ccggacaggt cgagccgacc ttcccgatcc
     3181 tgatgttctg catcgcgtac gggctgtcca tggactacga ggtgttcatg atgtcccgga
     3241 tcaaggagga gcacgaccgc accggcgaca acgtgcagtc cgtggcggtg ggcatcgagc
     3301 gcagcggacc gctgatcacc gctgccgcgg tgatcctggc gctcgccttc gccagctacg
     3361 gcgtctccgg cgtgatgtac ctgaagatgc tgggcatcgg catggcactg gtgatcctgg
     3421 tcgacgccac gctgatccgg gcggtgctgg tcccggtgtt catgcgcctg gcgggcaacg
     3481 cgaactggtg ggcgcccgcg ccgctgcgca agctgcacga gcggttcggc atcaccgagc
     3541 aggagacgtc gcggtccgag ccgcgcgtcc catccggcgc cgcgcactga gccggcgcag
     3601 acccaatcgg aaaaagggaa ccgtcctggc acccgcggca ggacggttcc cttcttggtc
     3661 tcgaagctca gtagcccatg ccgaagccgc cgctgaccgg gatcgccgcg ccggtgatga
     3721 aggaggcggc gtcgctggct aggaactcca ccaccccggc cacctcgtcg ggactgcccg
     3781 tacgacgcat cgccgtcatc cgcaggacct cttcgcgcct ggatgtggtg agctcagcag
     3841 tcatttcggt ctcgatcaga cccggtgcga ccaggttcgc ggtgatgccg cgcgagccga
     3901 gctcccaggc cagcgagcgg gtgagaccgg tcagcccgga tttggatgcg ctgtagttga
     3961 tctgcccggg cgctccggtg aacccgacga tcgaggagac gaagatgatc cggccccatc
     4021 gcgcgctgaa catcttgcgc gcggctcgcc gcgcgacccg gaacgaaccg gtcaggttgg
     4081 tctccagcac atcggagaag tcatcgtcgc tgagccgggg caacagcttg tcacgggtga
     4141 tcccggcatt ggccaccagc acctcgaccg gcccgtgttc ttcttcgatc cggcgaaaca
     4201 gttgctcgac gtcgtcgggg gcggtgatgt ccgcgcgcac ggcgaggaaa tcctccggtg
     4261 gcggctcgga tcggtaggtg atcgccaccc ggtgcccggc cttcgcgaag gactgggcga
     4321 tttcccggcc gatgccccgg ttcccgccgg tgaccagcac tgacctagtc atgttcccac
     4381 cctttgccgg tccagttcgg cagctgcgtc gtcgctgctg gtgaagtgca ctgcctgcca
     4441 gccggtttcg cgcgcacccg cgcagttctt ggccatgtcg tcgacgagca cgcactcgcc
     4501 gaccgggaaa ccgcagcgct caacggcgag cccgaaaata gcggcttccg gtttgcggct
     4561 gcccacctcg taggacagta cgacggagtc gaatagctcc ggcgacacca tgcgccgcca
     4621 ctgctcgtcc cagctcggcg gcatgttcga cagcaacccg acgaacactc cctgctcgcg
     4681 gagcttccgc agcgtgccga cccaggagga gttcgccggg cggccggcga accagcgctc
     4741 cccgagccgc gagagatcca cctcggctcc ggtgtcctcg cgaaccacgg ccgtcacctg
     4801 ccgagcccag tcagcttcgg tggtcagcgg agtgtccagc ggtgccatca catcgtccac
     4861 gccgtagcgg gcagcgacgt tctgcatggc tgcccacagc actgcggggg gagcgccggc
     4921 ctgtgccgcg aaccccgcga agctctcgtt gaccggtgga gtcagcaccc cgccgaagtc
     4981 ggtccacact gcccggaacg tcatgcagcc acctcgctgg acgtgccgac ctggtcgaac
     5041 ccgacggtgg tggtggcgat cacggtgccg tcctgcacga agtgcacgtc gagttcgttg
     5101 ctgtgctctc cgacgatcct caggtcgaca ggggagtcca gttccgcgaa gcggatgaac
     5161 tcggcgctga acgacgtgca ccgcacccgg gtcgcggccg cgccggtgcc gtcgtcgagc
     5221 gccagcagcg agagctggcg tgccgcttcg aacaggctca tcgccgggta gtggtcgtat
     5281 tcgtggtcga acaggctgcg gttgcgcggg ttgacgccca gttgcgccga cgtggagtgg
     5341 tggtccctgc gggcgttggt cagcaccacg ttctcgggca gcttgcgccc gaagaggtgc
     5401 gggctgagcg gcaccccagc cggggtcgcg ggcagctcgg acgatgtcgg cggttcgccg
     5461 gtgcgctgcc gggtccgcag ggcccggaag gttgtctttg cgaccgcggt ggcttcgatg
     5521 cgcgcggtcc cgatcgatcg cccggacaac gtaagctccg cgtccattcc catgttgcgg
     5581 ggtattccgg cgcgcatctc gacgtcggtg aacacgccgc tcatccgcaa ttcaccgggc
     5641 cgggtgccga tgacgagtcc gtccaggtcg gtgatccgga tgctcgccgt ctttactagc
     5701 atcgtggagt tcatcggcag gccgagcagt tcgtgggtgc cataggtgcc gccctggcgg
     5761 cagcactcca gcagcagcag cggatcgaac agctccggtc gagccgcgtg atcggtgtag
     5821 taggcgtggt agaggggtag ctgcgcatct accaggaact cgcgtcggcc agccgcattg
     5881 aatcccgtga tgaacacctc ggacagcgcc caccgatgta ccatttctcg ggcgacagtg
     5941 tggctgaagg cgcccacagg cggtgctaat tcaccggcat ccttgctcat cttttacctc
     6001 ctggaaatgc cgttaccagc ggccgatgct cggtgccgca atgttcgccg gacaaagtgc
     6061 aatgtgaaga ttttgcgaag ttttcgctgc aaatcctgcc ctgcttcgac gagcaattgc
     6121 acgttcctgc ggttgctttg ttgaccgcat ctattcttga attcgcgccc agctttgtaa
     6181 atatgtttcg catatgccgt ttgactgtgc cctcggtgat gcgcagggtg ctggcgattt
     6241 gccggttgct caacgcctgc gcgacgaggt tcacgatctc ccgctctcgc gcggacagtt
     6301 tgtggtggca gtgcatgcgg gcccggatca attcgatctg ctcggtggtc acctggccag
     6361 gcggcggagc gatccggatc tggtccagta gcgtgagcag ttcctcgagc gtggtttgcg
     6421 cgtccccgaa ggaggtgacg cccacggtcc gcagttcgcc cagcagctcg gtgtctctca
     6481 ttggaggcag ggccaggatc cgggtctgcg gcgaagtcaa ccggatccgc gcgatgttgg
     6541 ccatcaggta gttgaccgag gtcgtcgagc agaccagggc cacatccggc ggccgcgggt
     6601 cggccgcgcc gtcgaacgcg gagatcgcgc tcgggtcttc caccacttcc aggttgccga
     6661 aaggctgcaa tgcacgtgcc accgattggt gcaacagcac gttctcgatg cataagagta
     6721 atcgggtact aaggacgaac cctggatcgc tcataagtgc ccccaggcat gattgtgtgt
     6781 ggtacggttg aaagtgtgat cgtatgcagt cgctgaccaa aggctagggc acgacaacgc
     6841 gacgacttta gctcaatgcg agcgatcgct cgcaagtttg tgattcacat catgtaactg
     6901 cctgggttct tgactgaagc ttcggagttg ttcagctctg cccggccggc cgatgtcacc
     6961 taagtagtat tccgtactgg atttccggcc gtaagaatgg cctctcgggg aaccgctggg
     7021 gtttcctgga cgcatcttcg tggtgcgcgc gggccggcta tggggacgtc aatgtcgccg
     7081 tcctatgcgt caatcggagc gctccatacg gcgaaataga gtaatcgaag agttgtgcgg
     7141 gaggctgtgc gtgagcataa agtcgggcgg cacgttggta gtcggagccg ggcccaccgg
     7201 gctcaccgtc gccaccgagc tcatccgccg agagcgtccg gtacgcatca tcgaccgagg
     7261 agaacgcccg cacccgcatt cgcgggcgat cgtgctctgg ccgcggggcc tggaagcact
     7321 gcgccgctgc ggtgccgccg aagagatcgt cgaacgtgcg ctgccgttga aggcagggaa
     7381 ctactacacc ggcgggcgcc gggtggcccg actggtgttc tccggcgggc tggccggaac
     7441 ccgctaccag gccccgctat cgctgccgca gcgagacacc gaagaggtgc tgcgccgcca
     7501 cttcgagcaa ctcggcggca agatcgagta cggcgtgtcc atgagatcgc tgcgtcagga
     7561 ggcggagaag gtccacgtcg ggcttgggac cacttccggt acggccgatg aggaattctc
     7621 ctgggttgtg ggctgcgatg gtgcgcacag cggcacccgg gaacaactcg gggtggaatt
     7681 cgaaggccag gcgtaccagg agacgttcgc gctttccgac ggtgaatgcg aaaccccgct
     7741 ggcgcacaac gaggcgcatt acttcatgag cccccacggc gtgctcgtcg tggtggggct
     7801 gcccggcggc ctgtaccggg tgttcgtgag cctgcgaccg ggaaccgatc agtccgatct
     7861 gaacggtctg gtgcaggaag cagcgcagca gcgctgtccg gttccgctgc gcctggtcgg
     7921 cgagcagcag tccggtagct tccaggtcaa ccgccgcgtc gcaaagcgat tccacgaagg
     7981 ccgggtgatg ctcgccggcg atgctgcgca cgtgcacagc ccggccggtg gtcagggtat
     8041 gaacaccggc atcgaggacg cccagtccct cggctggcgc ttggcgtccg ccgatcccgg
     8101 cacggcggga gtggccggag tgcgtgcatg ggcggccgag cgtcgccacg tcgcacagca
     8161 ggtcgtcgcc gataccgacc ggcaaacgca aatgtggatg ctcaagggct ggcgccgccg
     8221 agcccgcgac ggggccctgg ggatcgccgc ccgaaccggc ctgctcaacc gcgttctgcc
     8281 accgcgtctg gcccagctcg cgctggccta tccgagcagt ggaaccggtg tcggcatgct
     8341 caaacccggc gtgcggctgc cggacgaaaa gctgggcgac aagtggttgc acgacatgct
     8401 gaacggcaac gccctggtcc tgggcttcgc ggtcgatcgg cgggaaacgg gcaacctgcc
     8461 cgtggtgcgt gctgccgcgg atgcgctcgc ccagcagtgg gccgcgcaac tggggtccga
     8521 ggccgatgtc gcgatcgtcc acagcggcgc accggacatg cccggcgacc tgctcgacga
     8581 aaccgggcag gtgcaccgcc ggttcggcat caccgggcct gcgctggtag tggtccgccc
     8641 ggacgcgatc gtggcgcacg ccgaacggtt gaccgacgac atcggacgtt gacgctgaca
     8701 tcactgacga ccaatcccgg gggcgaaatg cgcggcaacg aaaacgttct ggacggaacc
     8761 gacgacgtta tggacgaact caagaagttg gtcaccgagc tcaccgatct accagccggg
     8821 gcgctgacgt cggccgcgtc gctggcgacg gacctcggcc tcgacgatct ggcggtcgcg
     8881 cagatcctcg tcgaggcaga gctccggttc ggtgtccaga tcccggatcg catcgtgcgc
     8941 ccggggttgc aggaactcgc tgactacatc cgagccgagg cgcggaagga aaacacatga
     9001 acctgagact gggcctgcaa ctgacgagct tcagctaccc cagcaccccg ccggcgagcc
     9061 tctttgagcg gattgtggaa accgcccgaa ccgccgaaca ggcgggcttt gacctgttcg
     9121 cggtgatgga ccacctgatg caactgcccg tgcagggggc cccggacgaa ccgatgatgg
     9181 agccctacac gctgctgggc ggggttgcgg cgcgtactgc gcggatccag ctgggcaccc
     9241 tggtcaccgg cgtgacctac cacaacccag cgcggctggc caagaccgtt accgcgctgg
     9301 acatcgtctc cggtgggcgg gcgttgctcg gcatcggcgc cgcctggtac gacgaagagc
     9361 accgccgcta cgggtactca tttccgagcg tgcgggaacg gttcgcctgg ctcgacgagg
     9421 cgctgcagat ctgccgatcg atgttcgagg gcaagtcgac gtcgttcagc ggcgagcact
     9481 tctcggtgtc cgacgcgttg aacgaaccgc aaccggtgca cgtgccgccg atcctcatcg
     9541 gcggggccgg cgagcaacga acgatcccct tggctgccaa gcacgccgac gtcctcaatc
     9601 tgaactgcgg tttccccgac gtgccacgga agctcgaggt tctgcgggag aagtgcgcgg
     9661 agctcggccg cgactacgac gcgatcaaca aaaacgtgct cgcggtggtg ttcctcgggc
     9721 ggacgaacga ggaagcggtg gccaagcgcg acgccgtgct ggcccggcag cggtccggcc
     9781 ccgccgccac gggcgccggg ctccctgcgg cgctggggag ccggatgatc gttggcgacg
     9841 cggacacggt gggcgagcag atcagcggtc tgatcgacct cggtctcgac ggtgtgatcg
     9901 tcaatctgac gggcgacagc cacaacccgg agcggctggc ccatgcaggt gaagtgttgc
     9961 gggcggcgct gccttaatac tgcgacggca ggccgcaagc caggcgaggg cgattcctcg
    10021 accctccacc gggatccggc gacccagacc agattcagca gggtgcagcg gaggggcccg
    10081 tggcggtgca agctcagtcc cacaccgaat gcggcttgcg ccgcattccg aagcgatttc
    10141 tcaggccccg atcagcggtg tcaagtagtc gcggcagatc cgcttgagct cgccgatcat
    10201 agcggcgcgt tccgcgccct tggtaccgat gatcaacggc agcacgcccc ggaacacccc
    10261 gaccagcacc cgcgcgctgc gcacccgctg gtccttgggc agttccgggg cacgcgcggc
    10321 gaagatcgcg ctcagccgct cgatcaccgc ctcgcggacg tgcagcggct tggtcgccac
    10381 ctgctcgtca ggggcgttga acagggcctg gaacccgggg ttggacaggt tgaagtcgat
    10441 caccgggtcg accatcgcgt cgatcagctc ggtcagcggc agatcggcgt gctcgacagc
    10501 gagggccttg cgttgagtct gctgcaactg ccgcaggtac cgctcggaca gcgcacgggc
    10561 gatctccccc ttgttcggga agaactggta gagcgtgccg ggggaagacg cggcgcgcgc
    10621 ggcgatcgcg ttggtcgtcg cggcggcgta gccgacctcg ccgaacacct cggcggcggc
    10681 gtcgaggagc agctcgatcc gtcgttcacc ccgtgcctgc ctccggcgct gcggcggctg
    10741 atccgccatg accgtcacct cccgcgcatc cgcctggtgg taatgaagcg aaccttcact
    10801 cgtgttctcc cccgacacta acgcgtcgcg ccacacttcg ggatcttcgg ggaaggaccg
    10861 gcaccccgct gtcttcgact gctgcgtcgc caacatgccc ggtgatgttc cgcaggcctc
    10921 cacgcgtgcc ctgaccaacg cgacgccgcg acgaacgact ggggatgcgc cattgcgatc
    10981 ctccaattca
//
