LOCUS       NZ_CP040605            10411 bp    DNA     circular CON 25-AUG-2024
DEFINITION  Saccharopolyspora sp. ASAGF58 chromosome, complete genome.
ACCESSION   NZ_CP040605
VERSION     NZ_CP040605.1
KEYWORDS    RefSeq.
SOURCE      Saccharopolyspora sp. ASAGF58
  ORGANISM  Saccharopolyspora sp. ASAGF58
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 8045912)
  AUTHORS   Guo,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2019) Grain Processing Research Group, Academy of
            National Food and Strategic Reserves Administration, Baiwanzhuang,
            Beijing 100037, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.15x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012277335.1-RS_2024_08_25
            Annotation Date                   :: 08/25/2024 04:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,606
            CDSs (total)                      :: 7,535
            Genes (coding)                    :: 7,265
            CDSs (with protein)               :: 7,265
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 80 of 270
            Pseudo Genes (incomplete)         :: 228 of 270
            Pseudo Genes (internal stop)      :: 14 of 270
            Pseudo Genes (multiple problems)  :: 48 of 270
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:54:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2967014
            Orig. end                         :: 2977425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040605.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10411
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10411
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10411
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5411](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5411)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            200..664
                     /locus_tag="FDZ84_RS14040"
                     /old_locus_tag="FDZ84_14065"
     CDS             200..664
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS14040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_14065"
                     /product="hypothetical protein"
                     /protein_id="WP_168586257.1"
                     /transl_table=11
                     /translation="MLEWYHRSNEGAFLSGLIVSAIVFVILCVMDWGFDWIWTAPWLWM
                     IVVAVAPLWGLWIISRNRWGISAGADWLAIKSGYVDLYELTEVKVEGTSGGLSWDIELT
                     DKKATELWLDAKELQANQALWDLVYNGIRYSVQRGSAKTNPRALDKLKLR"
     gene            complement(665..931)
                     /locus_tag="FDZ84_RS14045"
                     /old_locus_tag="FDZ84_14070"
     CDS             complement(665..931)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308262.1"
                     /locus_tag="FDZ84_RS14045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14070"
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="WP_168586258.1"
                     /transl_table=11
                     /translation="MNGQRVPGGVVHELPADLRKALIANTTAFDAWKDITPLARNEFIC
                     WVEDAKQVITRERRIRRTQEELAEGKRRPCCWPGCKHRERTGK"
     misc_feature    complement(890..892)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1111..1404)
                     /locus_tag="FDZ84_RS14050"
                     /old_locus_tag="FDZ84_14075"
     CDS             complement(1111..1404)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS14050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_14075"
                     /product="hypothetical protein"
                     /protein_id="WP_168586259.1"
                     /transl_table=11
                     /translation="MHEHPYAATLSAIKSLHVQWSEEAELTSDAAHAAVLYRRLLELAD
                     TVARLFVYEQFHLEQASNTIPPQQLVLITGTEPGAGNPVRDETGSVPAGAAS"
     misc_feature    1390..1403
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.81"
                     /tool="antismash"
     gene            1670..2956
                     /locus_tag="FDZ84_RS14055"
                     /old_locus_tag="FDZ84_14080"
     CDS             1670..2956
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308266.1"
                     /locus_tag="FDZ84_RS14055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14080"
                     /product="hypothetical protein"
                     /protein_id="WP_168586260.1"
                     /transl_table=11
                     /translation="MSRQEHSDLLPPVHEAWLPREHPLHRPRHGRRQLAALLCAVLFFT
                     APVVTWVFGARAEQLENRPLAAFPSITDGWGFFTGLNAWATDNLPFRKDAVQSVDALSR
                     GVFGEPARLAGGSHTSPVGAGQVDEKPPIDENVFPDVIEGKNGWLYLGHDVSYRCVPKR
                     TTDQVIAGLRRWRSVVEASGRKFQLVIAPDKSTEYPENMPDDYAGKDCSTKAREEFWRR
                     VPAATGAVDLRAQLREVAARNQRPIYHDIDTHWTHEGAITMTYQLAERLQPGVTSSWKV
                     LPSRQYPHSADIPDLLGQDRTVPIQAYSLAPDGGAADNTQFKPSDFRQPLHLESRPKPG
                     MIAQPVRMVGDSFTQFASPYLAATFANITITHPDNVADDPVAAGELLAEGEVVTFELSE
                     RFVAGGRYPMLDPAVADQVGAVLAAHPVR"
     gene            complement(3891..4793)
                     /gene="thpD"
                     /locus_tag="FDZ84_RS14060"
                     /old_locus_tag="FDZ84_14085"
     CDS             complement(3891..4793)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="FDZ84_RS14060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14085"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_168586261.1"
                     /sec_met_domain="PhyH (E-value: 4.5e-36, bitscore: 123.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVADLSTQDRYPTRVDAESAMIRRSDPTVWPGVTAGPATAEELA
                     GHEAKGFHVVEGLLSPAEVQTYWQELERLTRDPEVRADERTIVERSSDEVRSIFEVHRM
                     SELIAELVRDPRVLDRARQILGSDVYVHQSRINYMPGYRGTGFYWHSDFETWHAEDGMP
                     AMRAVSISIALTDNYPFNGGLMIMPGSHRTFVSCVGETPDDNYKSSLKEQRVGVPTEDD
                     ITKLAYEHGIEQFTGQAGSALWFDSNCMHGSGNNITPFPRSNIFVVFNSVENALVNPFA
                     AGHPRPGFIASRNPTPILR"
     gene            complement(5001..5411)
                     /locus_tag="FDZ84_RS14065"
                     /old_locus_tag="FDZ84_14090"
     CDS             complement(5001..5411)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308276.1"
                     /locus_tag="FDZ84_RS14065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14090"
                     /product="ectoine synthase"
                     /protein_id="WP_168586262.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-54, bitscore:
                     181.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRHLSDIEDTDADIKTENWRSKRIVLAKEKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITEGEGEIEDKATGEVHQLKPGSLYLLNNHDKHQVRPRTTMRTV
                     CVFNPPVTGREVHDEDGVYPLVVEDDETPVAG"
     gene            complement(5518..6765)
                     /gene="ectB"
                     /locus_tag="FDZ84_RS14070"
                     /old_locus_tag="FDZ84_14095"
     CDS             complement(5518..6765)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (23-350). E-value: 2.1e-70.
                     Score: 229.3. Matches aSDomain:
                     nrpspksdomains_FDZ84_RS14070_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947388.1"
                     /locus_tag="FDZ84_RS14070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14095"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_168586263.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.8e-70, bitscore:
                     234.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDIFATLESEVRSYSRTWPTVFDRAGGSYLYDEDGTAYLDFFAG
                     AGALNYGHNNPLLKRKLIEYIERDGVTHSLDQSTVAKRAFLETFRDNVLKPRGYNYKVQ
                     FPGPTGTNSVESALKLVRKITGRESIISFTNAFHGMTLGSLSVTGNSMKRGGAGIPLVH
                     ATPMPYDDYFDGQVPDFLYFEKMLEDSGSGLNEPAAVIVETLQGEGGINSSRPEWLRGL
                     YDLCQKHGMLMIVDDVQMGCGRTGPFFSFEEAGIQPDIVCLSKSIGGYGLPMALTLIKP
                     EHDVWEPGEHNGTFRGNNPAFITATEALKQYWADDALEKSTLAKGERVGEVLEEIAAKY
                     GGAVAKGRGLARGLGFEDTNVAGKVSKAAFDRKLLLETSGPSSEVLKIMPPLTVSDDEL
                     EQGLQIVRESVQAVLA"
     aSDomain        complement(5716..6696)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FDZ84_RS14070_Aminotran_3.1"
                     /evalue="2.10E-70"
                     /label="FDZ84_RS14070_Aminotran_3.1"
                     /locus_tag="FDZ84_RS14070"
                     /protein_end="350"
                     /protein_start="23"
                     /score="229.3"
                     /tool="antismash"
                     /translation="DRAGGSYLYDEDGTAYLDFFAGAGALNYGHNNPLLKRKLIEYIER
                     DGVTHSLDQSTVAKRAFLETFRDNVLKPRGYNYKVQFPGPTGTNSVESALKLVRKITGR
                     ESIISFTNAFHGMTLGSLSVTGNSMKRGGAGIPLVHATPMPYDDYFDGQVPDFLYFEKM
                     LEDSGSGLNEPAAVIVETLQGEGGINSSRPEWLRGLYDLCQKHGMLMIVDDVQMGCGRT
                     GPFFSFEEAGIQPDIVCLSKSIGGYGLPMALTLIKPEHDVWEPGEHNGTFRGNNPAFIT
                     ATEALKQYWADDALEKSTLAKGERVGEVLEEIAAKYGGAVAKGRGL"
     gene            complement(6855..7421)
                     /gene="ectA"
                     /locus_tag="FDZ84_RS14075"
                     /old_locus_tag="FDZ84_14100"
     CDS             complement(6855..7421)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806913.1"
                     /locus_tag="FDZ84_RS14075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14100"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_246869004.1"
                     /transl_table=11
                     /translation="MTGDKPSHNIDNGNGRSGSAGRLEIDTPVVSDGPELYRITRDSAV
                     LDVNSSYAYLLWCRDFAQTSAVARIDGAVVGFVTGYVRPDAPDTLVVWQIAVDASQRGG
                     GVAGRLLGHLVDRVLPHGVRYLETTITADNAASISLFTALARKRAANLERSELFTADLF
                     PDAHLGEDLYRIGPFEPVPAAAAGS"
     misc_feature    7393..7410
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.97"
                     /tool="antismash"
     misc_feature    complement(7530..7542)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.72"
                     /tool="antismash"
     misc_feature    7648..7662
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.76"
                     /tool="antismash"
     misc_feature    complement(7649..7662)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.02"
                     /tool="antismash"
     misc_feature    7761..7774
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            8315..8806
                     /locus_tag="FDZ84_RS14080"
                     /old_locus_tag="FDZ84_14105"
     CDS             8315..8806
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS14080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_14105"
                     /product="hypothetical protein"
                     /protein_id="WP_168586264.1"
                     /transl_table=11
                     /translation="MAEPDSWSERERMEKRKAQDERVIQEWKRTNGGNSDALKHVRKFV
                     EHVRGDIGVDGVIVDVDAIPTNGGYEIKAGYYMELDPLNNLQALGSSAAQLSAQSAGGA
                     CVLGTSFEGDDMVPQGGVGRSYADGGPPSGGGQVGRRVRSEDGVDEAPHTASGSATASA
                     "
     gene            complement(9117..9464)
                     /locus_tag="FDZ84_RS14085"
                     /old_locus_tag="FDZ84_14110"
     CDS             complement(9117..9464)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FDZ84_RS14085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FDZ84_14110"
                     /product="hypothetical protein"
                     /protein_id="WP_168586265.1"
                     /transl_table=11
                     /translation="MKSSFRSVVRGVAISSALGAALVLGQAGIAHAATPEVFKLCSGGN
                     FDSFATFTERGGFSTFVVRRGTCQSFKYGGTHAERVDLKVANTGKYIGSLAYDGRVGAN
                     VRTVNDPSFTF"
     gene            complement(9693..10169)
                     /locus_tag="FDZ84_RS14090"
                     /old_locus_tag="FDZ84_14115"
     CDS             complement(9693..10169)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308295.1"
                     /locus_tag="FDZ84_RS14090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FDZ84_14115"
                     /product="DUF2127 domain-containing protein"
                     /protein_id="WP_168586266.1"
                     /transl_table=11
                     /translation="MAAETTMTDKLFRVAVLLKGLDGAVQLIGGVLLIFLPPDIVTRFA
                     HAVVTRDLLGPPAGSLAGHFEEAAQHFASGGSTFVIAYLIAHGVIKIGLVIGLLLKIMP
                     LYPVALTALGLFVIFEVLRAVQTRSIALPFFAALDVVIIILVLREYLELRRRSS"
     misc_feature    complement(10132..10146)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.94"
                     /tool="antismash"
     misc_feature    10376..10389
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    10377..10390
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     misc_feature    10377..10391
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.72"
                     /tool="antismash"
ORIGIN
        1 acatcgggaa gaagctcgaa gagatcaacc cgaagatgac ccggtaagga ctgctgtgac
       61 tgccacaccg ccacgtccga acccgaaaac cggcgaacca cggccacccc aagcgccaag
      121 gctaaccgtt tggggagatc taccgtcacg gtcaccgcgg ttcgcgcgtt tcccgccggc
      181 gccagaagga cgggggccag tgttggagtg gtaccaccgc tccaacgaag gagccttcct
      241 gtccgggctg atcgtttcgg cgatcgtgtt cgtgattttg tgcgtcatgg actggggctt
      301 cgactggatc tggacagcac cctggctgtg gatgatcgtc gtggcagttg cccccctctg
      361 gggcctctgg atcatctccc gcaaccgctg gggcatctca gcaggagccg actggctcgc
      421 gatcaagagc ggctacgtcg acctctacga actcaccgaa gtcaaggtcg aaggcacaag
      481 tggtgggctg tcgtgggata tcgagctgac tgacaaaaaa gccaccgagc tctggctcga
      541 cgccaaagaa ctccaggcaa accaggcgct ctgggacctc gtctacaacg gcatccgcta
      601 ctcagtccaa cgcggcagcg ccaagacaaa cccacgtgcg ctggacaaac tcaagctgcg
      661 gtaactactt gccggtgcgc tcgcggtgtt tgcatccggg ccaacagcag ggccgacgtt
      721 tgccttccgc cagctcctcc tgggtccggc gaatacggcg ctctcgggtt atcacctgct
      781 tggcatcctc gacccagcag atgaactcgt tgcgggccag gggcgtgatg tccttccagg
      841 catcgaatgc cgtggtgttg gctatcagcg ccttgcgcag gtctgcgggt aactcgtgga
      901 ccaccccacc gggcactcgc tggccgttca tagacccacg gtagcggacg aagcgagccc
      961 aagacggata cgctcagtcg ccgccacgcg ccagcatgta agggcgaccc cgcaggagcc
     1021 gccgtgatgg agatctgggc cgcggagacc tgactccact gaacctgggt gagccgtcga
     1081 agaacggcgc tgggcaggtc gccgaagtga tcagcttgcc gctccggctg gcaccgaacc
     1141 cgtttcgtca cgtacggggt tgcctgctcc tggttccgtt ccagtgatca aaacaagttg
     1201 ttgcggcggg atcgtgttgc tcgcttgttc gaggtggaac tgttcgtaga cgaacagccg
     1261 ggctacggtg tcggcgagtt cgagcaggcg acggtatagc acagcggcat gcgcggcatc
     1321 ggacgtcagt tctgcttctt cggaccactg gacgtgtagt gacttgatcg cggaaagcgt
     1381 tgccgcataa ggatgttcat gcattgccgt acgccttccc cagaccgctg ctacgagttg
     1441 ttgcaggtca ggagtaacac gctattgagg gacccgcaaa agggtcatct cggcacccga
     1501 tcacataccc gccgatcgga cgtcgtcggt tggcgccccg cgccgctgtg catgggggac
     1561 tacgacgact acgcgcggac gctaccgccg gacccgctcg cgcgccgctg tgcaggagat
     1621 ccgcgagttc tgggccggtc gcgctgcgtg cccggttaac gtgggttccg tgtcgcggca
     1681 ggagcactcc gatcttcttc ccccggtcca cgaggcgtgg ttgccgcggg agcatccgct
     1741 gcaccggccc cggcacggca ggcgccagct ggccgcgctc ctttgcgccg tgctgttctt
     1801 caccgcgccg gtggtgacct gggtcttcgg ggcgcgcgcc gagcagctgg agaaccgccc
     1861 gctggccgct ttccccagca tcaccgacgg ttgggggttc ttcaccgggc tgaacgcctg
     1921 ggccaccgac aacctgccgt tccgcaagga tgccgtgcag tcggtggacg cgctcagccg
     1981 cggcgtgttc ggcgagcccg cccggttggc cggcggctcg cacacctcgc cggtcggcgc
     2041 cgggcaggtg gacgagaagc cgccgatcga cgagaacgtg ttccccgacg tcatcgaggg
     2101 caagaacggc tggctgtacc tcggccacga cgtgtcctac cggtgcgtgc cgaagcggac
     2161 cacggaccag gtgatcgccg gactgcgccg ctggcggtcc gtggtggagg catcgggccg
     2221 gaagttccag ctggtcatcg cccctgacaa gtccaccgag tacccggaga acatgccgga
     2281 cgactacgcc gggaaggact gctcgacgaa ggcccgcgag gagttctggc gccgcgtgcc
     2341 ggccgcgacc ggcgcggtcg acctgcgtgc ccagctgcgc gaggtcgcgg cgcgcaacca
     2401 gcggccgatc taccacgaca tcgacacgca ctggacccac gagggcgcca tcaccatgac
     2461 gtaccagctc gccgagcgcc tgcagccggg cgtgacaagc agctggaagg tcctgccgtc
     2521 ccgccagtac ccgcacagcg ccgacatccc ggacctgctc ggacaggacc ggaccgtgcc
     2581 gatccaggcg tactcgctgg cgcccgacgg cggagccgcg gacaacacgc agttcaagcc
     2641 cagcgacttc cgccagccgc tgcacctgga gtcgcggccg aagccgggca tgatcgccca
     2701 gccggtgcgg atggtcggcg actcgttcac ccagttcgcc agcccgtacc tggcggcgac
     2761 gttcgcgaac atcaccatca cgcacccgga caacgtcgcg gatgacccgg tggcggcggg
     2821 cgaactgctc gccgaaggcg aggtggtcac gttcgaactg tccgaacgtt tcgtcgccgg
     2881 cggccgctac ccgatgcttg acccggcggt agccgaccag gtcggcgcag tcctcgccgc
     2941 ccaccccgtg cgctgatcct tccgggattt cggcgagctt ccatcgaaat tcgcaatgcg
     3001 gaacgtccgg tcagcttcag cgcaactcgg cagtttttgt agccggacac acgtactatt
     3061 tttgccgatc gccgtgtcgt gcgagaccgg tatccgatag tctgcccgcg cattgcgatt
     3121 gcggatcaac tgtccgtagc tgcacgtgca cgatgcgccc tggcggtgcg tcggctggga
     3181 tggggcgggc cgaagctggc acttcaaccc gccccggaag gggatcagct gtggtcgtcg
     3241 aaccgagtga cgagccacca caacgagacc cgcagtatca gcagcaggag cgcccgggtc
     3301 gtccgacgcg gccgggccgg gcgctttgct gcgccccgcc gacgacgagc ctgacgtgcc
     3361 ttccgggcct tcttgcggtt cacctgcatg ttcctttcgt tgttgatccc aacgttcggc
     3421 gtgcgccgaa cgcggcccga ctggtgccgg gcctgacaac aacaggacga gtcctgttga
     3481 ggcgctgcga caggacccat gccaggtggt accgcaggcc tcccttctga accccaggtt
     3541 ggaaacttcc cgacgatgac gggaattttg ataacggaca ccataggcca cggcgcgtaa
     3601 ccgtcgccac ccctatgggt gtgtcggata ctcttttctg gttggaatat tccgaagata
     3661 attacttacc gtcgagtaaa cccgctgggc ttcgatggtt tctcggctgg attagtcaaa
     3721 atggggtaat cgaacgattc cgaaattgac tgatcacgca gagctacaaa actatttgtg
     3781 atctgagtcg cggattggca accgaatcca tgtcacggcg cgaagcgaac gggcgagggg
     3841 cacccgcgac caaagtaact gggtcgcggg tgcccctcgc cccgggttcg tcagcgcagg
     3901 atcggtgtcg ggttgcggga tgcgatgaag cccgggcgcg ggtggcccgc cgcgaacggg
     3961 ttcaccaggg cgttctccac gctgttgaac accacgaaga tgttcgagcg cgggaacggc
     4021 gtgatgttgt tgccagatcc gtgcatgcag ttcgagtcga accacagcgc cgaccccgct
     4081 tggccggtga actgctcgat gccgtgctcg taggcgagct tggtgatgtc gtcctccgtc
     4141 ggcaccccga cccgctgctc cttcagcgac gacttgtagt tgtcgtccgg cgtctcgccc
     4201 acgcaggaca cgaaggtccg gtgcgagccg ggcatgatca tcaggccgcc gttgaagggg
     4261 tagttgtcgg tcagcgcgat cgagatgctc accgcgcgca tcgccggcat gccgtcctcg
     4321 gcgtgccagg tctcgaaatc ggagtgccag tagaagccgg tgccccggta tccgggcatg
     4381 tagttgatcc ggctctggtg cacgtacacg tcagatccga ggatctgacg ggcccggtcc
     4441 aggactcgcg ggtcccgcac cagctcggcg atcagctcgc tcatccggtg cacctcgaag
     4501 atcgagcgga cctcgtcgga gctgcgctcc acgatggtgc gttcgtcggc ccgcacctcc
     4561 ggatcccggg tgagccgctc cagttcctgc cagtaggtct gcacctcggc cggggacaac
     4621 aagccctcta cgacgtggaa gcctttggcc tcgtgccccg cgagctcctc cgcggtggcc
     4681 gggccggccg tcacgccggg ccagactgtg ggatcggagc gccggatcat tgccgactcg
     4741 gcgtccaccc gggtcgggta gcggtcctgg gtgctcagat cagcgacggt catggtttgc
     4801 gcctccttcg cgaattcccg ccggagcggc tggtgccggt ggcaccgccg ccttttctcg
     4861 gttcctcggt tagaagcggc gcattctgga tgcgcccgca attccccacg tccgcgcgtg
     4921 gggaagaccc gtcaacgcgc gtggcaggca accgtttccg gttacccgcc acgcgcggtc
     4981 aacaagctcg gtcgcccgga tcagcctgcg accggcgtct cgtcgtcctc gacgaccagc
     5041 gggtacacgc cgtcctcgtc gtgcacctcg cgcccggtca cgggcgggtt gaacacgcac
     5101 accgtgcgca tggtcgtgcg gggccggacc tggtgcttgt cgtggttgtt cagcaggtac
     5161 agcgaacccg gcttgagctg gtgcacctcg ccggtcgcct tgtcctcgat ctcaccctcg
     5221 ccttcggtga tgaacaccgc ctcgatgtgg ttggcgtacc agaagtcgtt caccgtaccg
     5281 gcgtagaggg tggtttcgtg caccgagaag ccgaccttct ccttggccag cacgatgcgc
     5341 ttgctgcgcc agttctcggt cttgatgtcg gcgtcggtgt cctcgatgtc cgagaggtgc
     5401 cggacgatca catttcctcc agtggatctt cgttctgctc tgccgcattg cagcgatcgg
     5461 cctgttcacg ccgtctggcg cggtgaacgg tccgatcgct gcgatgcggt tgccgagtca
     5521 ggccaggaca gcctgcaccg attcccggac gatctgtagc ccctgctcca gttcgtcatc
     5581 cgacacggtc aacggcggca tgatcttcag gacctcgctg ctgggtcctg aggtctccag
     5641 cagcagcttg cggtcgaacg cagccttgga gaccttgccc gcgacgttgg tgtcctcgaa
     5701 gccgagcccg cgcgccaggc cgcggccctt ggccaccgcg ccgccgtact tggcggcgat
     5761 ctcttcgagc acctcgccga cccgctcgcc cttggcgagg gtggacttct ccagcgcgtc
     5821 gtcggcccag tactgcttca aggcctccgt ggcggtgatg aacgccgggt tgttgccgcg
     5881 gaaggtgccg ttgtgctcgc ccggttccca gacgtcgtgc tccggcttga tcagcgtcag
     5941 cgccatcggc agcccgtagc ccccgatgga cttggacagg cagacgatgt cgggctggat
     6001 tcccgcttcc tcgaagctga agaacgggcc ggtgcggccg cagcccatct gcacgtcgtc
     6061 gacgatcatc agcatgccgt gcttctggca caggtcgtag agcccgcgca gccactccgg
     6121 ccgcgacgag ttgatgccgc cctcgccctg cagcgtctcg acgatcaccg cggcgggctc
     6181 gttgaggccg ctgccgctgt cctccagcat cttctcgaag tagaggaagt cggggacctg
     6241 gccgtcgaag tagtcgtcgt agggcatcgg ggtggcatgc accagcggga tgccggcacc
     6301 gccgcgcttc atcgagttgc cggtgaccga aagcgagccc agcgtcatgc cgtggaaggc
     6361 gttggtgaag ctgatgatcg attcccgccc ggtgatcttg cggacgagtt tcagcgccga
     6421 ctccaccgag ttggtgcccg tcgggccggg gaactggacc ttgtagttgt acccgcgcgg
     6481 cttgagcaca ttgtcccgga aggtttccag gaacgcacgt ttggccacag tggactggtc
     6541 gaggctgtgg gtcaccccgt cgcgctcgat gtactcgatc agttttcgtt tcagcaacgg
     6601 gttgttgtgc ccgtagttga gcgcgccggc accggcgaag aagtccaggt aggcggtgcc
     6661 gtcttcgtca tagaggtagc tgccgccggc gcggtcgaag accgtcggcc aggttcggct
     6721 gtagctgcgg acttccgatt ccagggttgc gaagatgtcg ttcacgatcc tccgttcggc
     6781 cgggcagcgc gcgcttcgcg cgccccggca ccctcaagaa caagattgcg tgccggtacg
     6841 gacggcggca gcggtcagga tccggccgcg gcggccggga cgggctcgaa cggcccgatg
     6901 cggtacaggt cctcccccag gtgcgcatcc gggaacaggt cggcggtgaa cagctcgctg
     6961 cgctccaggt tcgccgcgcg cttgcgcgcc agcgcggtga acaggctgat cgacgcggcg
     7021 ttgtccgcag tgatcgtcgt ctccaggtag cgaaccccgt gcggcagcac gcgatccacc
     7081 aggtggccca agagccgacc tgccacgcca ccaccgcgct gggatgcatc gacggcgatc
     7141 tgccacacga cgagcgtgtc gggggcgtcg ggccggacat acccggtcac gaatcccacg
     7201 accgcaccgt cgatgcgtgc caccgcggag gtttgcgcga agtcccggca ccacaacaaa
     7261 tatgcgtagg acgagttcac atccagcact gccgaatcgc gagtgattcg gtagagctca
     7321 gggccgtccg agacgaccgg agtgtcgatc tccaaccgtc cggctgaacc gcttctgcca
     7381 ttcccgttgt cgatgttatg gctcggttta tcgccggtca tgctttgcca acttaacaga
     7441 acatccgtaa cgcgccaggc tgtcggcgcg ataactatcg atctaccagg cgagacgcaa
     7501 ttttacacgt gagaagcact acagaagcgt tgcaatttgc ttgaagttga tgctacggag
     7561 cctgttgtcg attatgaatt tgatcatttg tcggctccct gacctgcgga aacgacgttt
     7621 ctaaagattc ccgaagtgaa ccttacccct ttttcgaaaa ttcaaactcc gatattcagg
     7681 tgaacatgag aagaggcggc tgccccaggc ttgcacgcgc ccgggcaggc cgcctcccgt
     7741 gagctgctac acgcccgcca tggactggat ccaatcgcgg tgcccggtga cgtcggggta
     7801 ggcgaacagc cgggccgtcc agggtgcgct gtccagggcg tgtgcaaggt tgacctgtcc
     7861 ggtttaggta gtttcggcta gggaggtggg tggcaggttg gccaggtggt ggaccgtttc
     7921 gataccggcg gctttgccgt tcgggcggcg ccagcgccat atccggatgc cctgccgggc
     7981 cccgagcaga tcggttcgca ggtttccgtc cagaacaagg atcttccagg ctgcgggccc
     8041 tggcacgtcc gtgcccgccc acgatttcca ttgaaattgc ggaggttgtc cacaggcatc
     8101 ggcgtgtcgg ggtgcgacac gccgacggtc cgcaagttcc attgaaatcg aaccttcgag
     8161 ttcagacgag gtcgtccggc agtgggtcag cggcggtgct ggcctggcga cgcccctgcg
     8221 tagcagccag gcgcgtttga accatgtgcg cctcgataca cgtgtaccga ctgacaggtc
     8281 ggagcgcaac gggaggactg gttccgtgaa ccatatggca gaacccgaca gctggagcga
     8341 gcgcgagaga atggagaaaa ggaaagccca agacgagcgc gtaattcagg agtggaaacg
     8401 aacgaatggc ggcaacagcg acgcattgaa gcacgtgcgc aaattcgtcg aacacgtgcg
     8461 cggggacatt ggggtggacg gcgtaatcgt cgacgttgac gccataccga ccaacggggg
     8521 ctacgagatc aaagcgggtt attatatgga gctggacccg ctgaacaatc tgcaagcttt
     8581 gggctcgtct gcggctcagt tgtcggccca gtcggctggc ggcgcatgtg tgctgggcac
     8641 gtcatttgag ggggacgata tggtgcccca gggaggagtg ggacggtcgt acgcggacgg
     8701 cgggccgcct tccggcggcg gtcaggtcgg tcggcgagtt cgcagcgaag atggcgtgga
     8761 tgaagcgccg cacacggcgt cgggttcggc taccgcatcg gcctgatgcc ggcggcacgg
     8821 agcggcaaag ccgcccgccg cgacccgggc ggcgttgccg gcacgtccgg gacacgagcg
     8881 ggccacgaag ctgagaaacg cgcggttgcg cgttgtggaa agtcccgaag atcccaccga
     8941 aagccaggcc taaatcgcca caatagaccg acgcgggaac aggccctgga ccgatggatc
     9001 aactggacgc gggttgaatg cactgaggtg atctcaataa gaaatggtgt cgtatagcaa
     9061 gagaagtgac actgtcactg aggcttactc ggttggggca tgcaattccg gtgtggtcag
     9121 aacgtaaagc tggggtcgtt gacggtgcgc acgttcgcgc ccacgcgccc atcgtaggcg
     9181 agagagccga tgtacttgcc ggtgttcgcc accttaaggt caactcgttc agcatgggtg
     9241 ccgccatact tgaacgactg gcaggtccca cgcctgacga cgaaagtcga gaacccgccc
     9301 cgctcggtaa atgtcgcgaa agagtcgaag ttgcccccgg agcacaactt aaagacttca
     9361 ggcgtggcgg catgggcgat gccagcctgc ccgaggacaa gagcggcacc gagcgcgcta
     9421 gagatagcca ctccgcgaac gaccgaacgg aaagatgact tcattatatt ctcctttggg
     9481 ttgtaacgta aagacgtttg aaacagagct cctggcagcc ggacaagaat cccaacgtga
     9541 agaggctatt cgtccctcgg aaactgccct atctaccatg aatggtcgac tggaaccaga
     9601 ggtgtaacca ccgccggccg aactgccccc ggtaacaaga cgtgcgctgt ccgcctaccg
     9661 gccaccgatg atcttggtcg agaggccgaa ggtcacgagg atcggcggcg caattcgagg
     9721 tattcccgga gcacgaggat gatgatcacc acatccagcg ccgcgaagaa cggcagcgcg
     9781 atcgacctcg tctgcaccgc gcgtagcacc tcgaagatca cgaacaggcc cagggcggtc
     9841 agtgccaccg ggtacagcgg catgatcttg agcagcaggc caatcaccag gccgatcttg
     9901 atcacgccgt gggcgatcag gtaggcgatt acgaacgtgc tgccgccgct ggcgaagtgc
     9961 tgcgcagctt cctcgaagtg cccggccagg gatccggccg gcgggcccag caggtcccgc
    10021 gtgacgacgg cgtgcgcgaa ccgggtgacg atgtcggggg gcaagaagat cagcagcacc
    10081 ccgccgatca gctgcaccgc gccgtccagt cccttgagca gcacggcgac gcggaacagc
    10141 ttgtccgtca tcgtggtttc ggcagccatg aagccatgat gcgcgctgaa tcccggacca
    10201 gcgatcttgc gcgttgccag ccaccggcgg cccgcatccg ggccgcccgc tcctcgaccg
    10261 gcaggtccgc gaatcggatc gtgcggcttc ccggccgatg atcgagaaga gttcggtgaa
    10321 cgacagggca aattggtaaa taccactttt cttcaaatct tgtttccggt cgcgaaattt
    10381 gtcataaaag ttcttggtgg acgacgagcg a
//
