LOCUS       NZ_CP050976            12947 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8910931
            Orig. end                         :: 8923878
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12947
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="39"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12947
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12947
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7947](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7947
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            858..1865
                     /locus_tag="HEP86_RS39150"
                     /old_locus_tag="HEP86_39150"
     CDS             858..1865
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091462.1"
                     /locus_tag="HEP86_RS39150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39150"
                     /product="chitinase"
                     /protein_id="WP_348772269.1"
                     /transl_table=11
                     /translation="MTAVSLLAGCSSEEPAKPKRPDPVTPSPHSAPPSSAAPTPKTGGF
                     SPYVDTSLKPSFDLVKAAQETGVKEYNLAFVSPGGGCVPKWGGRQPLAANPVARQADAL
                     RAQGGDVRISFGGQSGNELARVCGSVDKLVNAYRKVIEAYHLTKVDFDIEGPALTDTKA
                     NDLRAQAIARLQQDTKLDVSVTLPVLPSGLNRHAVAVLKSAKKNGARISSVNIMAMDYG
                     TYYDGDMGKYATAAATATQKQIKGALGIRDEATAWKSVGVTPMIGVNDVKGEVFRPDDA
                     AELKKFADEKGLGRLSMWSVTRDKPCPGGANLKAVATCSSLGKKTNEFVRAFTG"
     gene            2369..2809
                     /locus_tag="HEP86_RS39155"
                     /old_locus_tag="HEP86_39155"
     CDS             2369..2809
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEP86_RS39155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEP86_39155"
                     /product="hypothetical protein"
                     /protein_id="WP_168483300.1"
                     /transl_table=11
                     /translation="MLTPERPCGLALVTDRDGNYLAVIDPLSHRVVPTLSAPAPHMARC
                     DAGGPVVCTVVDGTDLAASRDSAALLGALSALRNTSVHGEGWRRFAAAAGPGLMAERNQ
                     FSGVFPDQGSTSGGPAGGHHRDGLRGRVGSGHAHRYGHLRLR"
     gene            2730..4730
                     /locus_tag="HEP86_RS39160"
                     /old_locus_tag="HEP86_39160"
     CDS             2730..4730
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /locus_tag="HEP86_RS39160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39160"
                     /product="Ig-like domain repeat protein"
                     /protein_id="WP_168483302.1"
                     /transl_table=11
                     /translation="MDITATVSAAGSGAGTPTGTVTFDFGDGTPTVTAPVTGGTATVTH
                     AWASTSGSPYALTAGYSGDADFSPSTATGMQTVGQAATTTAVASSPDPSVTGQPVSITA
                     TVSAAAPAAGTPTGTVTFDFGDGTPATLTSLSDGVARITHTYTAAGGPYTVTALYLGND
                     SFSSSSGGDTQTVGKAATTTAVVSSPDPTVVGQLTTVTASVASVAPGAGALSGTVTFNF
                     GDGSPTLTAPVSNGLAVVTHAYGSTAGSPFAITATYDGDANFTSSSGSDTQTVGKAATT
                     TSVISSPDPTVVGQSTTLTASVASVVPGAAAPTGTVTFSFGDGTPSTTTTLSDGIATVA
                     HTYTTTSGSPFPLTAIYNGDANFTSSSAGDSQTVGRAATTTAVVSSPDPSVAGQAVTLT
                     AMVAAVAPGAGTPTGTVTFSFGDGTSAGTATLSGGTATVTHTYATRSGSPFAITATYNG
                     DANFTASTGNDTQTLTKNASTTTVTSTPDPSVSGGTVTIRATVSSAAGTPTGTVTFSFG
                     DGTSTATATLSGGVATVTHPYTTTTGSPFTVTATYGGDASFNGSTGTDTQTVTRATTST
                     TVISTPNPSTTGDRVTVTATVVPVAPATGTPTGTVTLAITGRSPQTVPLVNGTASASFN
                     PLPKGTHLVTGNYNGDVSFAPSSGTTLQTVN"
     misc_feature    4979..4989
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    4982..4997
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.3"
                     /tool="antismash"
     gene            5001..6161
                     /locus_tag="HEP86_RS39165"
                     /old_locus_tag="HEP86_39165"
     CDS             5001..6161
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608091.1"
                     /locus_tag="HEP86_RS39165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39165"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_168483304.1"
                     /sec_met_domain="DAO (E-value: 7.6e-66, bitscore: 221.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSRRSSDVIVVGAGVVGAACAYYASRAGLSVTVVDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELDLALLSTGLWQELAELLPPQIEYEPKGGLVVAADETGMRAL
                     REFAAGQRAVGVRADEIPADRLRELEPHLTPVLAGGFHYPQDAQVMPALAAAHLLRAGG
                     DRVCLRLGEEVTGLLTTADGAVRGVRTTGGEVFAPYVVNAAGTWGGEFARLAGVELPVL
                     PRRGFVLVTEPLPRVVRRKVYAADYVADVASGSAALQTSAVVEGTAAGPVLIGASRERV
                     GFDRTLSTEVLRRLAVQATALFPVLAGVRAMRTYVGFRPYLPDHLPAIGPDPRVPGLLH
                     ACGHEGAGIGLAPATGLLIARSLSGAEPPLDPGPFHPARFEAEAAV"
     gene            6189..6503
                     /locus_tag="HEP86_RS39170"
                     /old_locus_tag="HEP86_39170"
     CDS             6189..6503
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091458.1"
                     /locus_tag="HEP86_RS39170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39170"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_168483306.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.6e-23, bitscore: 81.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTPAELAGANPGASFEITMDGHPVPALPGQSVAAALWSAGILA
                     WRTTRTTGRPRGAFCGIGQCYDCLATINGEPNRRACLVPASPGDAITTQEGHGHARIGV
                     "
     misc_feature    6234..6247
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            6484..7947
                     /locus_tag="HEP86_RS39175"
                     /old_locus_tag="HEP86_39175"
     CDS             6484..7947
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091457.1"
                     /locus_tag="HEP86_RS39175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39175"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_168483308.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.8e-24, bitscore:
                     84.3, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.5e-10, bitscore:
                     40.2, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPASASDPYDLAVLGAGAAGLAGAVTAAELGLSVALLDAAPQTGG
                     QFYRHPAPAVGATRPEALHHDWAAFAELRRRLERSDIRRLSGHHVWAVTKEDGDLWAVH
                     AVTGPDGNGEQAVRVRARAILLATGAYERQLPFPGWTVPGVVGAGGAQAMLKSGLVLPG
                     KRIVVAGSGPLLLAVASSLAAAGAQVPAVIEASGYLEYARHPGALAANPRKLVEAAVHG
                     AALLRHRVRLRTRSAVTEVHGTDRVAAVTVTRLDRDWRPVRGTGRQIACDALAVGHGLV
                     PQIELATALGCATRRTADGTSALALDALQETSVRGLWAAGETGGVGGAPLARTEGELAG
                     LAAAAWLHGRPADEGRVRALRRRRDRMRAFADAMAARHAPAAGWTGWLTGDTEVCRCEE
                     VTAGTIREAVTDLGARDARTVKLLTRAGMGWCQGRMCGTAAACLAAGDGEAEPPAERRP
                     FAVPVPLSALASSDDSTGGRPDAVPAVDD"
     misc_feature    7985..7995
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            8019..8927
                     /locus_tag="HEP86_RS39180"
                     /old_locus_tag="HEP86_39180"
     CDS             8019..8927
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091456.1"
                     /locus_tag="HEP86_RS39180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39180"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_168483310.1"
                     /transl_table=11
                     /translation="MTALTWNSTRPWRGIMVATALPLRDDLSVDHDAYAQHVRWLIEQG
                     CDGVVPNGSLGEYQTLTDEERARVVRTAVEAAGDGARVMPGVAAYGSAESRRWAEQAGE
                     AGCGSVLLLPPNAYRADERAVRAHYAEAAKAGVPVVAYNNPLDTKVDLTPALLARLHQD
                     GSIVAVKEFSGDVRRAYEIAELAPDLDLLIGADDVLLELAVAGAVGWVAGYPNAFPATC
                     AALYHAAVAGDLDTALPLYKSLHPLLRWDSKTEFVQCIKLSMDLVGRHGGPARPPRSPL
                     TGETETEVRAATEKALAAGHH"
     gene            8978..9982
                     /locus_tag="HEP86_RS39185"
                     /old_locus_tag="HEP86_39185"
     CDS             8978..9982
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="HEP86_RS39185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39185"
                     /product="proline racemase family protein"
                     /protein_id="WP_168484289.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGVLPGATMAERRLHFIEHL
                     DHLRTLLMYEPRGHSAMSGAILQPPTRADADYGVLYIEVSGLLPMCGHGTIGVATVLVE
                     SGMVPVTEPVTPVRLDTPAGLVAVDVHVADGVAKSVTLTNVPAFCAGLDRTVEVPGYGR
                     VTYDLAFGGNFYAFVELDALGLPFDRARKDDLLAAGLAVMAAINASPDRPVHPEQSEIA
                     GVKHVYLAAPGSDAQHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLDRDFVNE
                     SFIGTRFTGRLVAETEVGGRPAVVPTVTGRAWITGTAQYFLDPDDPFPGGFLL"
     gene            9979..11361
                     /locus_tag="HEP86_RS39190"
                     /old_locus_tag="HEP86_39190"
     CDS             9979..11361
                     /EC_number="1.2.1.-"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091454.1"
                     /locus_tag="HEP86_RS39190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39190"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_168483312.1"
                     /sec_met_domain="Aldedh (E-value: 2e-122, bitscore: 407.9,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAVSSVTSRNPADPSDVLVSVPAPGAFAAADAVERARAAQPGW
                     LAGGAAARSAALGAVATALETAADELAALAVREVGKPLTEARAEIARSVAIWRYYAQAP
                     YEPLGAVHETAVGPGLLLTRRRPYGVAGLLTPWNFPFAIPSWKAAPALAAGNAVVLKPA
                     PEATACARRLASLIEQALPEGVCTVLPGGATEGNALVSVADVISFTGSTTVGRSVVQAA
                     TARGIPVQAEMGGLNAALVLPDADIEQAARHIAAAIAGYAGQKCTATRRVIAVGAALGP
                     LREALVEALRAVPVGDPADPATVCGPLINEQARDRVSRARDGLPVLAGAAAPGLPGWYA
                     APTLVEQVPPGHQLLREEVFGPVAALLPADDLAHAVRITNSVPYGLVTSVHSAGLDPVL
                     HGLDQLDTGMIRVNAPSTGVDFHLPFGGAKESGHGPREQGRAALEFYTSSRTYTLSTVG
                     TM"
     gene            11409..12095
                     /locus_tag="HEP86_RS39195"
                     /old_locus_tag="HEP86_39195"
     CDS             11409..12095
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091453.1"
                     /locus_tag="HEP86_RS39195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_39195"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_168483314.1"
                     /transl_table=11
                     /translation="MGHLKHRDLNATRERLRDQVAHALRAALISGELQPGEVYSAPVLA
                     EDFGISATPVREAMLDLAREGLVEPVRNKGFRVTEVNERDLDQYTEIRALIEIPMIGRI
                     TRSAARADLEALRPVAEEIVRAARDHDLIGYLEADRQFHLDLLGLSGNDRLVETVGDLR
                     KRSRLYGLTALDERGELIPSAEEHLELLDLMLAGEAKAAEKCMAKHLGHVRSLWARSGT
                     AAKSGR"
     misc_feature    12160..12170
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    12229..12244
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.16"
                     /tool="antismash"
     misc_feature    12608..12622
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
ORIGIN
        1 ctggtggtcg gcggagacgc ccggtacgtc ggagagcagg acccgggtgg tgtccccggg
       61 gcgcggtgcc gggcgtacgg cggcgagctt gccctcggga tgggccttcc gggcgtcggc
      121 gacctgctgc gacagcggcc gctcggtctt gcccaccggc acgctcagct cctgcgcgta
      181 aaggagcttc tccgcctgga aggaaccggc gtacagcagc ccggtgaccg cggcgacgag
      241 caggaagggc gcgatcagta ccccggcgta gaagtgcaga cgcaacacca gcgggcggaa
      301 cgcggcccac ccggaacgcc gtaccgcccg ctcctccccg gagccggtgg tgtccgggga
      361 gtccggtgca ccgttcggtg cgagacgttg ttcagtgggc acgcggggct cccggctcgt
      421 cgatgcggtg gcccggcccg ctgccggtac cgctcaggga gtcggacgaa gcggagcacg
      481 agttcccgtg ggtgatgtga gctgtcccat acgggaggct gccgggcccg cacccacccg
      541 ccaggatgtc ggcaccggat ggcacgatgg cggccatgac aaacgggccg accttacctg
      601 ctgacgtgct gatgtcgcgc gtgctgtgtg ggctgctgcg ccgggtcctt ccctcccgca
      661 cacaccgccc gaagttcccg gatccggccc gtggcagacg gagtcttccg cccgtcccga
      721 ccccgtactc caggtccgaa cagcactgcc caccccccac cgttggaggc agcccgatga
      781 tgacgcaccc cgcagacgca cacgcttccc ggcggagccg caagcggaag ccgaccgccc
      841 ttgcggcggt gacggccgtg acagcggtct ctctgctcgc cggctgctcg tccgaggaac
      901 cggcgaagcc gaagcgcccg gacccggtaa caccttcccc ccactccgcg ccgccgagca
      961 gcgccgcccc cacgccgaag accggcgggt tctcgccata tgtggacacc tcgctgaagc
     1021 cctccttcga cctcgtcaag gcagcccagg agaccggtgt gaaggagtac aacctggcgt
     1081 tcgtctcgcc gggcggcggc tgcgtaccca agtggggcgg ccggcagccg ctcgccgcca
     1141 accccgtggc gcggcaggcg gatgcactgc gcgcacaggg cggcgatgta cggatctcct
     1201 tcggcggaca gagcggcaat gaactggcca gggtctgcgg ctcggtggac aagctggtga
     1261 acgcctaccg caaggtcatc gaggcctacc acctgaccaa ggtcgacttc gatatcgagg
     1321 gcccggcact gaccgacacc aaggccaacg accttcgcgc gcaggccatc gcccggcttc
     1381 agcaggacac aaaactggac gtctccgtca ccctcccggt gctcccctcc ggcctcaacc
     1441 ggcacgccgt cgcggtgctg aagagcgcca agaagaacgg tgcccggatc tcctcggtga
     1501 acatcatggc catggactac ggcacgtact acgacggcga catgggcaag tacgccaccg
     1561 cggccgccac cgccacgcag aagcagatca agggggcgct gggtatccgg gacgaggcca
     1621 cggcgtggaa gagcgtcggg gtcaccccga tgatcggtgt gaacgacgtc aagggcgagg
     1681 tcttccggcc cgacgacgcg gccgagctca agaagttcgc cgacgagaag gggctcggcc
     1741 ggctgtcgat gtggtcggtc acccgcgaca agccctgccc gggaggcgcc aacctcaagg
     1801 cggtggcgac ctgttcgagc ctcgggaaga agacgaacga gttcgtgcgc gccttcacgg
     1861 gctgaaccgg gagaggggga aggggcgggc cgggcaccgc cgtaccgggg tgacatgccg
     1921 gggtcggctc accgcggttc atcggtgacc gggttccgct cgcccgatcg ggtgagctgg
     1981 gtcccgctgt gttgcctgca tctcaccggg atggcttact gaatccgtcc gattccggtg
     2041 ggccgcccat cctttccggg ggagtggaag gcgccctgcc tgtttcggag caccggtctg
     2101 atgacggtgc cgttcgagcg ctcctccgtt ggggatgtgc aggaccggcc atgtcggcgc
     2161 gcgtacgcgg gcgtctcatg gtgcggggag cccgcaagtt ggctggagcc gtcatgcctg
     2221 ctgcccgcgc tgtgtgcccc gtccgcgtca tcgccctccc ttgggtggcc ccgccgggcc
     2281 ggtacgctcc cttcccccaa cttccctatg aagaaagccc cgttgaggcg aacaccgcgg
     2341 gcacggaggc atcggcggcg acaccgtcat gctgactccg gaacgcccct gcgggctcgc
     2401 cctcgtcacc gaccgcgacg gcaactacct cgccgtcatc gacccgctgt cccaccgggt
     2461 cgtccccacc ctcagcgcgc cggccccaca catggcgcgg tgcgatgccg gcggcccggt
     2521 cgtctgtacc gtggtcgacg gcaccgatct ggcggcgtcg agggacagcg ccgcccttct
     2581 gggcgccctc tccgcgctgc gcaacacctc ggttcacggg gagggttggc ggcgtttcgc
     2641 tgccgcggcc ggccccggcc tgatggccga gcgcaaccag ttctccggtg tcttcccgga
     2701 ccagggctcc accagcggtg ggccagccgg tggacatcac cgcgacggtc tccgcggccg
     2761 ggtcgggagc gggcacgccc accggtacgg tcaccttcga cttcggtgac ggcaccccga
     2821 cggtcaccgc acccgtgacg ggcggcaccg ccaccgtcac ccacgcctgg gcgagcacct
     2881 ccggcagccc gtacgccctc acggccggct acagcggcga cgccgacttc agcccctcca
     2941 cggcaaccgg tatgcagacc gtcggccagg ccgcgaccac caccgccgtg gcctcctcgc
     3001 ccgatccctc ggtgacgggc cagccggtga gcatcaccgc gacggtctcc gcggccgcac
     3061 cagccgcggg caccccgacc gggacggtca ccttcgactt cggtgacggc actccagcca
     3121 ccctgacctc gctctccgac ggcgtcgccc ggatcaccca cacctatacg gccgcgggcg
     3181 gcccgtacac ggtgaccgcg ctctacctgg ggaacgacag cttctcctcg tcgagcgggg
     3241 gtgacacgca gaccgtgggt aaggcggcca ctaccacggc ggtggtgtcg tcgccggatc
     3301 ccacggtggt ggggcagttg accacggtga cggcttcggt ggcgtcggtc gcgccggggg
     3361 cgggggcgct gtcgggtacg gtcaccttca atttcggtga tggcagtccg acgttgacgg
     3421 caccggtcag taatgggctg gcggtggtga cgcacgctta cggcagtacg gcgggcagtc
     3481 cgttcgcgat caccgcgaca tacgacgggg atgccaactt cacctcgtcg agcgggagcg
     3541 acacccagac cgtgggcaag gcggccacca ccacgtcggt gatctcctcg cccgatccca
     3601 cggtcgtggg tcagtccacc accctcacgg cgtccgtcgc ctccgtggtg cccggggccg
     3661 ccgcccccac cggtacggtc accttcagtt tcggcgacgg caccccctcg accaccacca
     3721 cgctctccga cggcatcgcg accgtcgcgc acacctacac caccacgagc ggcagcccct
     3781 tccccctcac ggcgatctac aacggggacg ccaacttcac ctcgtcaagt gccggtgact
     3841 cgcagacggt gggcagggcg gcgacgacga cggcggtggt ttcttcgccg gatccgtcgg
     3901 tggcggggca ggcggtgacg cttaccgcga tggtggcggc ggtggctccg ggggccggta
     3961 cgccgaccgg tacggtcacc ttcagtttcg gcgatggcac cagcgccggc accgcgactc
     4021 tctccggcgg caccgcgacc gtcacccaca cctacgcgac caggtccggc agccccttcg
     4081 ccatcacggc cacctacaac ggagacgcca acttcacggc gtccaccggc aatgacaccc
     4141 agacgctcac caagaacgcg tccaccacca cggtgacctc cactcccgac ccctcggtgt
     4201 ccggcgggac ggtgaccatc cgggcgacgg tctcctccgc ggccggtacg ccgaccggca
     4261 cggtcacctt cagtttcggt gacggcacca gcaccgccac cgcgaccctc tccggcggag
     4321 tcgccaccgt cacccacccc tacacgacca ccaccgggag cccgttcacc gtcacggcca
     4381 cctacggcgg ggacgccagc ttcaacggct ccaccgggac cgacacccag accgtcaccc
     4441 gggccacgac ctccaccacc gtgatctcca cgcccaaccc ctccacgacg ggtgaccggg
     4501 tcacggtcac cgcgaccgtg gtgcccgtcg cccccgcaac gggcacgccc accggaaccg
     4561 tcaccctcgc catcacggga cgcagccccc agaccgtgcc tctcgtcaac ggcacggcca
     4621 gcgcgtcgtt caacccccta ccgaagggaa cccacttggt caccggcaac tacaacggcg
     4681 acgtcagctt cgcgccgtcc tccggaacca ctctccagac ggtgaactga cggagcggtg
     4741 ccccggcccc ggctcccgtg agcggagccg gggcaccgct gtgttccggc cgcctcggga
     4801 agtgccaggc ggcctcccgc ggctctcccg gtgccgcgat ccggggcagc ggcgcctcgg
     4861 tccggttggc gcgtccgtta cttacccgcg cccggggagt cgtgaagaat ccggacgcga
     4921 cgacgcgctt cggcccggga gtggcgaccg gatcacgcgg cactcttggc agcgtcagtg
     4981 caatgtgaaa tattactgac gtgccctcga gaagaagctc ggatgtcatc gtcgtcggcg
     5041 cgggcgtggt cggcgcggcc tgtgcctact acgcgtcccg cgccggcctc tccgtcaccg
     5101 tggtcgaccg cggccccgtc gcgggcggca ccacgggcgc cggtgagggc aacctcctgg
     5161 tctccgacaa ggagcccggc cccgaactcg atctcgcgct gctctccacc gggttgtggc
     5221 aggagctggc cgaactgctc ccgccgcaga tcgagtacga gcccaagggc ggcctcgtgg
     5281 tcgccgccga cgagaccggg atgcgggccc tgcgcgaatt cgcggccgga cagcgcgcgg
     5341 tcggtgtgcg ggcggacgag atcccggcgg accggttgcg ggagctggaa ccgcatctga
     5401 ctcccgtcct ggcagggggc tttcactatc cgcaggacgc tcaggtgatg ccggccctgg
     5461 ccgccgccca tctgctgcgc gcgggcggtg accgggtgtg cctgcggctc ggtgaggaag
     5521 tcaccggcct gctgaccacg gcggacgggg cggtgcgggg agtgcgtacg acgggcggcg
     5581 aggtgttcgc cccgtacgtg gtcaacgcgg ccggcacctg gggcggcgag ttcgcgcggc
     5641 tcgcgggcgt ggaactcccg gtgctgccac gtcgcggctt cgtgctcgtc acggaaccgc
     5701 tgccgcgggt ggtccggcgc aaggtgtacg cggccgacta tgtggccgac gtcgccagcg
     5761 gctcggcggc gctccagacc tcggcggtgg tcgagggtac cgccgccggg cccgtcctga
     5821 tcggcgccag ccgggagcgg gtcggcttcg accggacgct gtcgaccgag gtgctgcgcc
     5881 gcctggcggt ccaggccacc gcactcttcc cggtcctcgc cggggtccgc gcgatgcgga
     5941 cgtatgtggg gttccgcccg tacctgcccg accatctgcc ggccatcggc cccgatccgc
     6001 gggtgcccgg tctgctgcac gcctgcggtc acgagggcgc ggggatcggc ctcgccccgg
     6061 ccaccgggct gctcatcgcg cggtccctga gcggggccga accgcccctg gacccgggcc
     6121 cgttccaccc cgcccgtttc gaagccgaag ccgccgtctg acccgtccgc tacgagagga
     6181 ggcccgcagt ggcccgtacc cccgccgaac tggcaggggc gaaccccggc gcgtcgttcg
     6241 agatcaccat ggacggccat ccggtccctg ccctgcccgg ccagtccgtc gccgcggccc
     6301 tgtggtccgc cggcatcctc gcctggcgca ccacccgcac caccggccgc ccgcggggag
     6361 ccttctgcgg catcgggcag tgctacgact gcctcgccac catcaacggg gagcccaacc
     6421 gccgggcctg tcttgtgccc gcgagcccgg gcgacgcgat cacgacccag gagggacacg
     6481 gccatgcccg catcggcgtc tgacccgtac gacctcgcgg tgctcggcgc gggcgccgcc
     6541 ggtctcgccg gtgccgtcac ggcagccgaa ctcgggctgt ccgtcgccct gttggacgcc
     6601 gcaccgcaga ccggcggcca gttctaccgg catccggcac ctgccgtcgg cgcgacccgg
     6661 cccgaggcgc tgcaccacga ctgggcggcc ttcgccgaac tgcgccgacg cctggagcgg
     6721 agcgacatcc gccggctgtc cgggcaccat gtctgggccg tcacgaagga ggacggcgac
     6781 ctgtgggcgg tgcacgccgt caccggaccc gacggcaacg gggagcaggc ggtccgggtg
     6841 cgggcccggg cgatcctgct cgcgaccggc gcctacgaac gccaactccc ctttcccggc
     6901 tggaccgtac cgggggtcgt gggtgccggg ggagcgcagg ccatgctcaa gtccggtctg
     6961 gtgctgcccg gtaagcggat cgtcgtggcg ggcagcggac ccctgctgct cgccgtcgcc
     7021 tcgtcgctgg ccgccgccgg tgcgcaggtc ccggccgtga tcgaggcgtc cggctatctg
     7081 gaatacgcac ggcatcccgg tgcgctcgcc gccaatccgc gcaagctcgt cgaggcggcg
     7141 gtccacgggg ccgccctgct gcggcaccgc gtccggttgc gcacccgcag cgcggtcacc
     7201 gaggtgcacg gcaccgaccg ggtggcggcc gtcaccgtca cccggctcga ccgcgactgg
     7261 cggcccgtac gggggacggg ccggcagatc gcctgcgacg ccctcgcggt gggacacggc
     7321 ctggtcccgc agatcgaact ggccaccgca ctcggctgcg ccacccgccg caccgcggac
     7381 gggacatcgg ccctggccct ggacgccctt caggagacct ccgtacgcgg gctgtgggcg
     7441 gcgggggaga ccgggggagt gggcggcgcc ccgctcgccc gtacggaagg ggagctggcc
     7501 gggctcgcgg cagccgcctg gctccatggg cgccccgccg acgaggggcg cgtacgggcg
     7561 ctgcggcgtc ggcgggaccg gatgcgtgcc ttcgccgacg cgatggctgc cagacatgca
     7621 ccggcggcgg gctggacggg ctggctgacc ggcgacacgg aggtctgccg ctgcgaggaa
     7681 gtgaccgccg gaacgatccg cgaagcggtc accgacctgg gcgcccgtga cgcccgcacc
     7741 gtcaaactcc tcacccgggc cggtatgggc tggtgccagg ggcggatgtg cgggacggcg
     7801 gcggcgtgcc tcgcggcggg cgacggggag gcggaaccac ccgccgaacg ccgcccgttc
     7861 gccgtgcccg ttccgctctc ggccctcgcc tcgtccgacg actccaccgg aggccggccg
     7921 gacgccgtcc cggccgttga cgactgaccg ccggccgtcc tcgtggcggc cacggacgac
     7981 ccaataaaat gtcacacgct accgaagggt tcaccggcat gaccgccctc acctggaaca
     8041 gcacccgccc ctggcgcgga atcatggtcg ccacggcact gccgctgcgc gacgacctgt
     8101 ccgtcgacca cgacgcctat gcccagcacg tccgttggct gatcgagcag ggctgcgacg
     8161 gcgtcgtacc gaacggctcg ctcggcgagt accagaccct caccgacgag gagcgggccc
     8221 gtgtggtgcg gaccgcggtc gaagcggcgg gcgacggcgc gcgggtcatg cccggcgtcg
     8281 ccgcctacgg cagcgccgag tcccggcgct gggcggaaca ggcgggcgag gcgggctgcg
     8341 gctcggtcct cctactgccc ccgaacgcct accgcgccga cgaacgggcc gtccgcgccc
     8401 actacgccga ggccgccaag gcgggggtgc cggtcgtcgc gtacaacaac cccctcgaca
     8461 ccaaggtgga tctgacaccc gcgctgctgg cccggctgca ccaggacggc agcatcgtcg
     8521 cggtcaagga attctccggc gatgtgcgcc gcgcctacga gatcgccgaa ctcgccccgg
     8581 acctcgacct gttgatcggc gcggacgatg tcctgctcga actcgccgtg gccggtgcgg
     8641 tcggctgggt cgccggctac cccaacgcct tccccgccac ctgcgccgcg ctctaccacg
     8701 ccgccgtcgc cggtgacctg gacaccgccc tgccgctcta caagtccctg cacccgctgc
     8761 tgcgctggga ctcgaagacg gagttcgtcc agtgcatcaa gctgtccatg gacctcgtcg
     8821 gccgccacgg cggccccgcc cgcccgccgc gctccccgct cacgggagag accgagaccg
     8881 aagtccgggc cgccaccgag aaggccctgg ccgccggaca ccactgaccg catgcgtcac
     8941 cactgaccgc atccgtgccg cttcgaaagg actcatggtg cgtacgcgtc atgtcttcca
     9001 cgccgtcgac tcgcacaccg agggcatgcc cacgcgcgtg atcaccggtg gcgtcggggt
     9061 gctccccggc gccaccatgg ccgagcgcag actgcacttc atcgagcacc tcgaccatct
     9121 ccggacgctc ctgatgtacg agccgcgcgg gcactccgcc atgagcggcg ccatcctcca
     9181 gccgcccacc cgcgccgacg ccgactacgg cgtcctctac atcgaggtct ccggcctgct
     9241 gccgatgtgc gggcacggca ccatcggggt cgccaccgtc ctcgtcgagt cgggcatggt
     9301 gccggtgacc gagccggtca ccccggtgcg gctcgacacc ccggccggtc tggtcgccgt
     9361 cgacgtgcac gtggcggacg gggtggcgaa gtcggtcacc ctcacgaatg tgcccgcctt
     9421 ctgcgccggt ctggaccgca ccgtggaggt cccgggatac gggagggtca cctacgacct
     9481 cgccttcggc ggcaacttct acgccttcgt cgaactcgat gcgctggggc tgccgttcga
     9541 ccgcgcccgc aaggacgatc tgctggccgc cggactggcc gtcatggccg cgatcaacgc
     9601 ctcgccggac cggccggtcc accccgagca gtcggagatc gccggggtca agcatgtcta
     9661 tctggccgct cccggctcgg acgcccagca ctcgcggcat gcgatggcga tccacccggg
     9721 ctggttcgac cgctcgccct gcggcaccgg cacctcggcg cggatggcgc agctgcacgc
     9781 ccgcggcgaa ctgcccctgg accgggactt cgtcaacgag tccttcatcg gcacccgctt
     9841 caccggccgc ctggtcgccg aaaccgaggt cggcgggcgg cccgccgtgg tgccgaccgt
     9901 caccggccgc gcctggatca ccggcaccgc ccagtacttc ctcgacccgg acgacccctt
     9961 ccccggaggc ttcctcctgt gaccaccgcc gtctcttctg tcacctcccg caatccggcc
    10021 gatccgtccg atgtgctggt gtcggttccg gcccccggag ccttcgccgc tgcggatgcc
    10081 gtcgagcggg cccgcgcggc ccagcccggc tggctggccg gcggtgccgc cgcccgctcg
    10141 gccgccctgg gcgcggtcgc caccgccctg gaaaccgccg cggacgaact ggccgcgctc
    10201 gccgtacgcg aagtgggcaa accactcacc gaggcacgcg ccgagatcgc ccgcagcgtc
    10261 gcgatctggc gttactacgc ccaggccccc tacgaacccc tcggcgccgt gcacgaaacc
    10321 gccgtcggcc cggggctgct gctgacccgc cgccgtccgt acggggtcgc cggcctcctc
    10381 accccctgga acttcccgtt cgccattccg agctggaagg cggccccggc actcgccgcg
    10441 ggcaacgcgg tggtcctcaa gcccgccccc gaggcgacgg cctgcgcccg gcgcctcgcc
    10501 tccctcatcg agcaggccct gccggaaggg gtgtgcacgg tgctgcccgg cggagcgacc
    10561 gagggcaacg cgctcgtctc cgtcgccgat gtcatctcct tcaccggctc gacgaccgtc
    10621 ggccggtcgg tcgtgcaggc ggccacggcc cggggcatcc cggtccaggc cgagatgggc
    10681 ggtctgaacg cggccctggt cctcccggac gcggacatcg agcaggcagc gcgccatatc
    10741 gccgccgcga tcgccggata cgcgggccag aagtgcaccg ccacccgtcg cgtgatcgcg
    10801 gtcggagcgg ccctcggccc cctgcgggag gcgctcgtgg aagccctgcg ggcggtgccg
    10861 gtcggcgacc cggccgaccc cgccaccgtg tgcggaccgc tcatcaacga gcaggcccgc
    10921 gaccgggtca gccgcgcccg ggacggtctc ccggtcctcg cgggcgcagc cgcgcccggc
    10981 ctccccggct ggtacgcggc cccgaccctg gtcgagcagg tgcccccggg ccatcagctg
    11041 ctgcgcgagg aggtcttcgg gccggtcgcg gcgctgctgc ccgccgacga tctggcccat
    11101 gcggtccgga tcaccaactc ggtcccgtac ggcctggtca cctcggtcca ctcggccggt
    11161 ctggacccgg ttctgcacgg gctcgaccag ctcgacaccg gcatgatcag agtcaacgcc
    11221 ccgtccaccg gggtcgactt ccatctgccg ttcggcggcg ccaaggaatc cggccacgga
    11281 ccgcgggaac agggccgcgc cgccctggag ttctacacct ccagccgtac gtacaccctc
    11341 tccaccgtgg gaacaatgtg aaattgtatg ctggtgatcc gctccgccag gaagggatca
    11401 ccaccgctat ggggcacctg aagcaccgcg atctcaacgc cacccgggag cggctgcgcg
    11461 accaggtcgc ccatgccctg cgagcggccc tgatctccgg tgaactgcag ccgggggagg
    11521 tgtactcggc gccggtcctg gccgaggact tcgggatctc ggccacgccc gtgcgcgagg
    11581 cgatgctcga cctggcgcgc gaaggcctgg tcgagcccgt ccgcaacaag ggtttccggg
    11641 tcaccgaagt caatgaacgc gacctcgacc agtacaccga gatccgtgcc ctcatcgaga
    11701 ttcccatgat cggccggatc acccgctccg ccgcccgtgc ggacctggag gcgctgcggc
    11761 cggtcgccga ggagatcgtg cgcgccgcgc gcgaccacga cctcatcggc tacctggagg
    11821 ccgaccggca gttccacctc gatctgctcg gcctcagcgg caacgaccgc ctcgtcgaga
    11881 ccgtcggaga cctgcgcaag cgctcacggc tctacgggct cacggccctg gacgagcggg
    11941 gcgagctgat cccgtcggcc gaggaacacc tcgaactcct cgacctgatg ctggccggcg
    12001 aggcgaaggc agccgagaag tgcatggcca agcacctggg ccatgtccgc tcgctctggg
    12061 ccagaagcgg gaccgcggcg aaatccggca ggtagtgggg cggcggcgag ccccgtccca
    12121 ctgcggacag cgatgccgcc cgcccttccg ccggtcccat gcaacgtcac acatcatgac
    12181 gcgcccccgg gccgtggaag ggacccatga accccgccta caccaccgtc gaccacttct
    12241 tcaccgtccc cctggaccac tccgccccca acggggaaca catacaggtc ttcgcccgcg
    12301 aggtcgtcga cccggcccgc gcggacgagc ggctgccctg gctgctgtat ctgcaaggcg
    12361 gtccgggcgg caaatccccc cgcccgtcgg ccggctcacc cggctggctg gaccaggccc
    12421 tccagaccca ccgggtgctc ctgctcgacc agcgcggcac cggccgttcc accccggtca
    12481 ccgcccgagc cgccgcccgc ttcacctcgc ccgcccaact cgccgcatac ctgggccact
    12541 tccgcgccga ctcgatcgtc gccgacgccg aactgatccg ccgccaactc tgccgggacg
    12601 aaccctggga gaccctcggc cagagctacg gcggcttcct caccctcacc tacctctccc
    12661 gggcccccga gggcctgcgg gcctgctacg tgaccggcgg tctgcccggc ctcaccgcca
    12721 ccgccgacga ggtctacgcc cgcacctacc cacgggtgcg ggaccgcgtc ctcgacttct
    12781 acgcccgcta cccggacgac gcccaccgcc tccgccacat cgccgatctc ctcacgacca
    12841 ccgaggtccg gctgcccgac ggagaccgcc tcaccacccg ccgactgcgc accctcggcc
    12901 tcgccctcgg catgggcgac ggtttcgaac ggctccactg gctgctc
//
