LOCUS       NZ_CP050976            11041 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8578612
            Orig. end                         :: 8589653
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11041
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="36"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11041
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11041
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6041](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6041)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(207..812)
                     /locus_tag="HEP86_RS37655"
                     /old_locus_tag="HEP86_37655"
     CDS             complement(207..812)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971478.1"
                     /locus_tag="HEP86_RS37655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37655"
                     /product="HD domain-containing protein"
                     /protein_id="WP_168482995.1"
                     /transl_table=11
                     /translation="MTMDDLVIPDTRACRAALEVASAYCSPALLNHSVRAYLWAAAYAS
                     AQDIAFDPELLYVSAMFHDLGLVKEFDSHTVPFDEAGGHVAWVFAAGAGWPVERRVRAS
                     EVVVRHMWDEVNVAMDPEAHLLVYSTSLDISGRRPDTLSADLRTEVLARYPRLGLGEEF
                     LACFRDQAARKPDSSAAAAVRQGIAARIAANPLDAYGA"
     gene            937..1164
                     /locus_tag="HEP86_RS37660"
                     /old_locus_tag="HEP86_37660"
     CDS             937..1164
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="HEP86_RS37660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37660"
                     /product="hypothetical protein"
                     /protein_id="WP_042158238.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     TGPAGSIALDDPRLVEWRGGSLDDWPMPPA"
     gene            1309..1479
                     /locus_tag="HEP86_RS37665"
                     /old_locus_tag="HEP86_37665"
     CDS             1309..1479
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEP86_RS37665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEP86_37665"
                     /product="hypothetical protein"
                     /protein_id="WP_168482996.1"
                     /transl_table=11
                     /translation="MSDDSGNGDDGSDSGYATRSGGGSGSFPDLSVLRRAMRWETFVLL
                     AALVILMAVAS"
     gene            1654..3174
                     /locus_tag="HEP86_RS37670"
                     /old_locus_tag="HEP86_37670"
     CDS             1654..3174
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092055.1"
                     /locus_tag="HEP86_RS37670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37670"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_168484247.1"
                     /transl_table=11
                     /translation="MNEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VAFFPTAAAAQGSGFRACRRCRPDAVPGSAEWNARADVVGRAMRLISEGVVDREGVAGL
                     AARLGYSARQVQRQLNAELGAGPVALARAQRAHTARVLLQTTALQAAEIAFAAGFASVR
                     QFNDTIKEIYALTPSELRAARPGKGTRFGPVAPPGTPGVLPLRLAFRGPYAARQLFDHL
                     QQRAITGIEEVSGEPGARTYRRTLRLPHGAGIAEVDEAAGDGWLDCRLHLSELRDLTTA
                     SQRVRRLFDLDADPYAVAERLRADDVLARLVDRHPGLRSPGAPAPDELAVRAVLGQQVS
                     VAAGRTLGGALVAAYGEPLPQPAGALTHLFPRPGDLAQAALAELGMPESRRATLRTLST
                     ALADGTVVLDAGADRDEAERALLGLRGIGPWTAAYIRMRALGDPDVLLTGDVAVLAGMR
                     RAGAPTAGLRERANAWRPWRSYAMHHFWNAPVTEPASPRPTTTESTGP"
     gene            3171..3683
                     /locus_tag="HEP86_RS37675"
                     /old_locus_tag="HEP86_37675"
     CDS             3171..3683
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="HEP86_RS37675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37675"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_168482997.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGTALASLSVPGQKGGATVQ
                     DGWTYAPAAFTGIAHQLRAYFDGELTRFALAYAPTRGTDFQRRVWQALEAIPYGTTTTY
                     GRIAADIGAARGAVRAIGTAIGANPLLVVRPCHRVIAANGSLSGYAGGPARKQQLLGLE
                     GAWPEVH"
     gene            <3824..4024
                     /locus_tag="HEP86_RS37680"
                     /old_locus_tag="HEP86_37680"
                     /pseudo=""
     CDS             <3824..4024
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092053.1"
                     /locus_tag="HEP86_RS37680"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37680"
                     /product="2OG-Fe(II) oxygenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="LLVEQRPRAQSRGTSTVLPPGHGLIFTTRDRPVRSARGWSAGPVR
                     HGVIIIRSGRRHSLGLVFHDA"
     gene            complement(4064..4828)
                     /locus_tag="HEP86_RS37685"
                     /old_locus_tag="HEP86_37685"
     CDS             complement(4064..4828)
                     /NRPS_PKS="Domain: PKS_KR (7-176). E-value: 8.1e-12. Score:
                     37.4. Matches aSDomain:
                     nrpspksdomains_HEP86_RS37685_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="HEP86_RS37685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37685"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_168482998.1"
                     /transl_table=11
                     /translation="MGDLNGKTALVTGSSRGIGRSIAQRLAQDGALVAVHYGSNDTAAK
                     ETAESITTAGGRAFLVGAELGVPGDVEALFAGFDAELAACGAEPGLDILVNNAALNFPG
                     RIDQVTPEEFDRTIAVNIKAPFFIAQHGLRRMRDGGRIINISSAVTSTAHPAQIAYSVS
                     KGGVDTLTLALAKDLGARGITVNTLSPGWVETDVTAARRATPEGRAALAAYSVFNRIGK
                     PQDIADVAAFLASDESRWITGQRIDVTGGSML"
     aSDomain        complement(4301..4807)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEP86_RS37685_PKS_KR.1"
                     /evalue="8.10E-12"
                     /label="HEP86_RS37685_PKS_KR.1"
                     /locus_tag="HEP86_RS37685"
                     /protein_end="176"
                     /protein_start="7"
                     /score="37.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRSIAQRLAQDGALVAVHYGSNDTAAKETAESIT
                     TAGGRAFLVGAELGVPGDVEALFAGFDAELAACGAEPGLDILVNNAALNFPGRIDQVTP
                     EEFDRTIAVNIKAPFFIAQHGLRRMRDGGRIINISSAVTSTAHPAQIAYSVSKGGVDTL
                     TLALAK"
     CDS_motif       complement(4736..4801)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEP86_RS37685_0001"
                     /evalue="1.90E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="HEP86_RS37685"
                     /protein_end="31"
                     /protein_start="9"
                     /score="17.3"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRSIAQRLAQDGA"
     gene            complement(5001..6041)
                     /locus_tag="HEP86_RS37690"
                     /old_locus_tag="HEP86_37690"
     CDS             complement(5001..6041)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="HEP86_RS37690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37690"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_348772267.1"
                     /sec_met_domain="AfsA (E-value: 2e-17, bitscore: 61.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRILTAPDLIPLRRLGAVMSDTMHVSGAVQPRTPPQTQPKRNPV
                     GSAGHRHGPGVPQEFVHRPFIDDILVTSWHRRDDSHFSLTAQWPHDHGYFTPVHGRHHL
                     ILTGETIRQAGLLLCHTELGVPVGHHFILGDLVYTTHPEHLAVGGGPTRLTIDVTCSRM
                     RMRAGRLTNGHFDMTIRKAGRIVATGHSDVTVASPAVYRRIRGERLTARRSLAPLPPAV
                     PHHLTGSADDRDVLLSPTGRPDRWRLRIAPGHAAMVNPANDHIPGMLLLDAAQQAARVL
                     TAPQEFVPYAFGTEFRRYAEHGMPCDIEARLVPSALPCTTTVQVTGFQDGRPVFVSTLT
                     ALDDRR"
     misc_feature    complement(6003..6005)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6027..6029)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6046..6059)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     misc_feature    complement(6129..6142)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.56"
                     /tool="antismash"
     gene            6160..6846
                     /locus_tag="HEP86_RS37695"
                     /old_locus_tag="HEP86_37695"
     CDS             6160..6846
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="HEP86_RS37695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37695"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_168483000.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRLILEAAASVFDDLGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLDEHVLDIAVEPQKIKLQEFVDSGQVLAYRLRSDPIQRGAARLA
                     VEQGSNHLDRKQSMLAWTRFVVELLNQARDRGEVMESVVVPETAELFVGAFAGIQLMSH
                     ALTNQADLSQRLTIFFQHTLPSIAVPAILAKLNLDPERGEKLDATLQGKALAHAGADAG
                     ADVVTG"
     misc_feature    6197..6206
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     misc_feature    complement(7256..7272)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.6"
                     /tool="antismash"
     gene            7463..9895
                     /locus_tag="HEP86_RS37700"
                     /old_locus_tag="HEP86_37700"
     CDS             7463..9895
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="HEP86_RS37700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37700"
                     /product="MFS transporter"
                     /protein_id="WP_168483001.1"
                     /transl_table=11
                     /translation="MTRTTTDQLPARAAETAATHPGPDPAVGGRRRSPGGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPVLLNTAPSNATWVMTATLLAGAVSTPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLVCGFTSDLVTMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGL
                     MSSSIGVGGGLALPVAALVAQHADWHTLFFGAAGLGVLSMGLTLLVVPETSVRAQGRFD
                     VAGALGLSAGLVALLLPLTKGSDWGWGSPTTLILFGVGVVILVLWGVMELRLADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCMAPLGL
                     TMMLVAPLYARIAARRGPKVSLLLGMLVIAIGYGAGIGLMDAAWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDASETGAANGLNTLMRSIGTSVSSAVIGMVLADMSQKMGPATVPTM
                     AGFRVSFLIATAAVLVGVALACFLPSQRKAAGPTLVAESTDGAVDAESAVELTSVDGQM
                     EPLAKSAPSWARPVTEPHQALTRPTPEATTASVDAVGFRGRVADTSGSPVPGASITLID
                     RQGRQAGLATADADGHFTLDAPTAGTYVLTGAAPGHTPHAASATYRGAGAATRVDLVLT
                     GTGRLGGTMLGGAEGTPLADGSIVVTDAEGAVVARTASGADGAWDISSLPPGHYTVVLS
                     AAGHQPQARAVDLSGGAPERQDARLQPTATVRGTVRGPHGRPVADAAVTLVEDGTVAGH
                     TVTGPDGAFAFSGLSGADYTLTAAGYPPHTAPISLTAGTHETLDLELDLAQPPIPGG"
     gene            complement(10083..10667)
                     /locus_tag="HEP86_RS37705"
                     /old_locus_tag="HEP86_37705"
     CDS             complement(10083..10667)
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298: putative carboxymuconolactone decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528860.1"
                     /locus_tag="HEP86_RS37705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_37705"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_168483002.1"
                     /transl_table=11
                     /translation="MPARIAPTEAPHPSEVAEVLHRMMPGGEEPIALFRLYARNLPLTE
                     ALHAWGSYQLSRRLSLGLRDREILIDRTCARCGCAYEWGVHIARFAPRAGLTPAQITSL
                     THGSSADTCWTTDRDRLLLDAADALHTGHDIDDTLWERLSAEFSPEQLLDLLMLCGWYH
                     AISFTARATRLAPEPGTPTFDDFLPPDPPKR"
ORIGIN
        1 gggcgggcgg ggggcgtgcc gtgcgcgagc ggggtgacgg tcatcacccc gccggcctgg
       61 aacgcgcagc tgttgttcac cgttcaattg ggtgtgggtg ctgcggcacc cctcccgagc
      121 cgccccggcc agcatccccc cgtctggccg gggcttcctc gtgccgcggg cgacggccat
      181 ggcccgcggg ccgcaggggg cgtccgctac gccccgtacg catccagcgg attggcggcg
      241 atccgcgcgg cgatgccctg gcggaccgcg gcggcggcgg aactgtccgg cttgcgcgcg
      301 gcctggtccc ggaagcacgc caggaactcc tcgccgagcc cgagccgcgg gtagcgcgcc
      361 agcacctcgg tacggaggtc cgccgacagc gtgtcgggac gccgcccgga gatgtccaac
      421 gaggtggagt acaccagcag atgggcctcc ggatccatgg ccacgttcac ctcgtcccac
      481 atgtgccgga cgaccacctc ggacgcccgc acccgccgct ccaccggcca cccggcgccc
      541 gccgcgaaca cccacgccac atggccaccc gcctcgtcga agggcacggt gtggctgtcg
      601 aactccttca ccagcccgag gtcgtggaac atggccgaca catagagaag ctccggatcg
      661 aaggcaatgt cctgggccga ggcgtaggcg gccgcccaga gatacgcccg cacggagtgg
      721 ttcagcagcg cgggggagca gtaggcgctc gccacctcca gcgcggcacg acaggcgcgg
      781 gtatcgggaa tcacaagatc atccatggtc atggccacga ttgtcgccca tgggtcatgg
      841 tccacgggcg agatcgccgg tcaccctcca agggccgttg acgcgcacgg cggctgccga
      901 aatgcgcgag cacggacccc ggcgcaggct ggatgcatga ccgagcatcc gcctgtcatc
      961 gtgcacccgc cgacgccgtc cggtggccga cgggtgaccg tgcgtgggca gatcgtcggc
     1021 ctggcccatg gccgcggcga tgttgccgag ttcctccgcc gcgcgggcgt caccggcccg
     1081 gccgggagta tcgcgctgga cgacccgcgc ctcgtcgaat ggcgcggtgg ctcgctggac
     1141 gactggccga tgccaccggc ctgaaggccc gcttgcggac tcgccccggc cctcgtctcc
     1201 gtccccgtag ccgtcacgcg gtgccgcacg gcagggcgat acgctgacga ccgacgatcg
     1261 gaaggtcgtc gtggctggtc aggaacggca ggagagcggg ggcagggcat gagcgacgac
     1321 agcggaaacg gcgacgacgg ttccgacagc gggtacgcga cgcgcagtgg cggcggatca
     1381 gggagctttc cggacctgag tgtcctgcgg agggcgatgc gctgggagac cttcgtgctg
     1441 ctggcggcct tggtgatcct gatggcggtg gcctcgtagt acgagtggga tacggcggaa
     1501 cttctgaact ccggttgatc tcttgggagt tgagccgttg cctgctgtcc ggtccgggtg
     1561 cccagggtgc gccgcgcagc cccacaccct ccgaacgctc cgtccgattt ccgccagcgc
     1621 cgccgtctga cctgctgcac agtggacact gtgatgaacg aggacagcag gtacgaggcg
     1681 gtgcgcagca gggacgcccg attcgacggt gccttcttct tcgcggtgtc cacgaccggg
     1741 atctactgcc ggccgagctg tcccgccacc acgccccgac gccggaatgt cgccttcttc
     1801 ccgaccgccg cggccgccca gggctccgga ttccgcgcct gtcggcgctg ccgccccgat
     1861 gcggtgcccg gctcggcgga gtggaacgcc cgcgctgatg tcgtcggacg ggcgatgcgg
     1921 ctgatcagcg agggtgtggt cgaccgggaa ggcgtcgccg ggctggcggc ccggctcggc
     1981 tacagcgccc ggcaggtcca gcggcagctg aacgccgagc tgggcgccgg gccggtggct
     2041 ctggcgcggg cacagcgcgc ccacaccgcc cgggtgctgc tccagaccac cgcactccag
     2101 gcggccgaga tcgccttcgc ggcgggcttc gccagcgtgc ggcagttcaa cgacaccatc
     2161 aaggagatct acgcgctcac cccgagcgag ctgcgtgccg cccgccccgg taagggaacg
     2221 cgcttcggcc cggtggcacc gcccggcacg cccggggtgc tgccgctgcg gctggccttc
     2281 cgcgggccgt atgcggcgcg gcagctcttc gaccacctcc agcagcgcgc gatcaccggt
     2341 atcgaggagg tcagcggcga gcccggcgcc cgcacctacc ggcggaccct gcggctgccg
     2401 cacggcgcag ggatcgccga ggtcgacgag gcggccggcg acggctggct ggactgccgg
     2461 ctgcacctgt ccgagctgcg ggacctcacc accgcgagcc agcgggtgcg ccgcctcttc
     2521 gacctggacg ccgatccgta cgcggtcgcc gaacggctgc gcgcggatga cgtcctggcg
     2581 cggctggtgg accggcaccc cggtctgcgt tccccgggcg ctcctgcccc ggacgagcta
     2641 gccgtccgtg ccgtactcgg ccagcaggtc tcggtcgcgg ccggacgcac cctgggcggc
     2701 gccctggtcg ccgcctacgg agagccgctg ccccaaccgg ccggtgcgct cacccacctc
     2761 ttcccccgcc ccggcgacct ggcgcaggcc gccctcgccg aactcgggat gcccgagtcc
     2821 cggcgcgcga ccctgcgcac cctgagcacc gccctcgccg acggcacggt cgtcctggac
     2881 gccggagccg accgggacga ggccgaacgc gcgcttctcg ggctgcgcgg catcggcccg
     2941 tggaccgccg catacatccg gatgcgcgcc ctcggggacc ccgatgtcct gctgaccggc
     3001 gatgtggccg tacttgccgg catgcgccgg gccggtgccc ccacggccgg tctgcgggag
     3061 cgggcgaacg cctggcgccc ctggcgctcg tacgcgatgc accacttctg gaacgcgccg
     3121 gtcacggagc cggcgagccc ccgccccacc accacggaaa gcaccggacc atgacgctct
     3181 acaccaccct cgacagcccg ctgggtgaac tcctgctggt gggcgaggaa tcggccaccg
     3241 caccgggcgg caccgcgctc gcatcgctgt ccgtacccgg gcagaagggc ggggcgaccg
     3301 tccaggacgg ctggacatac gcccccgccg ccttcaccgg catcgcccac cagctccgcg
     3361 cctacttcga cggagagctg acccgcttcg ccctcgccta cgcccccaca cggggcaccg
     3421 acttccagcg gcgcgtgtgg caggcactgg aggccatccc gtacggcacc accaccacgt
     3481 acgggaggat cgccgccgac atcggtgcgg cgcgcggtgc ggtacgcgcc atcggcacgg
     3541 cgatcggcgc gaacccgctg ctcgtcgtcc ggccctgcca ccgggtgatc gccgcgaacg
     3601 gttccctgtc gggctacgcg ggcgggcccg cacgcaagca gcaactcctc ggcctcgagg
     3661 gcgcttggcc ggaggtccac tgagccgccc cgtactcccg cgaccgccga gcgccgcacc
     3721 gacccacgcc cacaggagcg ctccatgacc accacgacca ccaagcgtca cgcccgcccc
     3781 ggcacactcg cccggcgtgt cgcggccgcc gactggacgg cgcctgctcg tcgagcagcg
     3841 gccgcgcgcc cagtcccgcg gcaccagcac cgtcctgcca ccgggccacg gactgatctt
     3901 cacgacccgt gaccgccccg tacgctccgc ccggggctgg tcggccggcc ccgtgcgcca
     3961 cggagtgatc atcattcgct ccggccggcg gcactccctg ggcctggtct tccacgacgc
     4021 ctgaagcggc cccggccgca agcggaccca ggggccggcg gcctcagagc atggagccgc
     4081 cggtgacatc gatgcgctgg ccggtgatcc agcgggactc gtcggaggcg aggaaggccg
     4141 cgacgtcggc gatgtcctgc ggcttgccga tccggttgaa caccgaatag gccgccagtg
     4201 cggcccgccc ctcgggcgtc gcccgccgcg ccgcggtcac atcggtctcg acccagccgg
     4261 gggacagggt gttcaccgtg atcccgcgtg cgccgaggtc cttggcgagg gcgagcgtga
     4321 gggtgtccac gccgcccttg gagacgctgt aggcgatctg cgccgggtgt gccgtactcg
     4381 tcaccgccga cgagatgttg atgatgcgtc cgccgtcccg catcctgcgc agaccgtgct
     4441 gggcgatgaa gaacggggcc ttgatgttga ccgcgatggt gcggtcgaac tcctcggggg
     4501 tgacctggtc tatgcgcccg gggaagttga gtgccgcgtt gttgacgaga atgtcgaggc
     4561 cgggttccgc accgcaggcg gcgagttcgg cgtcgaagcc ggcgaagagg gcttccacat
     4621 caccggggac ccccagctcc gcgcccacga ggaaggcccg cccacccgcg gtggtgatgc
     4681 tctcggccgt ctccttggcc gcggtgtcat tgctgccgta gtggacggcg accagggcac
     4741 cgtcctgggc gaggcgctgg gcgatgctcc ggccgatacc ccggctggat cccgtcacca
     4801 gcgcggtctt tccgttgagg tcacccatga gtgtgattgg ccttccgtgt acctgtggcg
     4861 ggcgcgctgc cgccgttgat catcaattgc tgtgctgcgg tggggcagtc ggggcggcgg
     4921 cccctgtcca gccgcttccg cctcctggcc cgtacggtgc ggataccggt cgcgcggcag
     4981 gcggctcgtg atccgtgtgc tcagcgccgg tcgtccagcg ccgtcagggt ggagacgaac
     5041 accggtcggc cgtcctggaa gccggtgacc tggaccgtcg tggtgcaggg gagcgcggac
     5101 gggacgaggc gcgcctcgat gtcgcagggc atgccgtgct cggcgtagcg gcggaattcg
     5161 gtgccgaagg cgtacggcac gaactcttgc ggcgcggtca ggacgcgggc cgcctgctgt
     5221 gcggcgtcga gcagcagcat ccccggaatg tggtcgttcg cggggttcac catcgcggca
     5281 tggccgggcg cgatacgaag ccgccagcgg tccggccgcc cggtggggga gagcaggaca
     5341 tcgcggtcat cggcgctgcc ggtcaagtgg tgcggtacgg caggggggag cggggcgagc
     5401 gagcggcgcg cggtcagccg ttcgccgcgg atccggcggt agacggcggg ggaggcgacg
     5461 gtgacatcgg agtggccggt cgcgacgatc cggcccgcct tgcggatggt catgtcgaag
     5521 tgcccgttgg tgagcctgcc cgcccgcatc cgcatccggc tacaggtgac atcgatcgtc
     5581 agccgggtgg ggccgccccc aacggccaga tgctcggggt gggttgtata caccaggtcc
     5641 ccgaggatga agtgatggcc caccgggaca ccgagttcgg tgtggcacaa cagcagcccg
     5701 gcctggcgaa tcgtctcacc ggtgagaatg agatggtgac ggccatgtac cggagtgaaa
     5761 tacccgtgat cgtgcggcca ctgtgcggtc agcgagaagt gggagtcgtc ccggcggtgc
     5821 catgaggtga ccagaatgtc gtcgatgaac ggacggtgca cgaattcctg cggaacgccc
     5881 ggaccgtggc gatgcccggc gctgccgacg gggttccgct tcggctgcgt ctgaggtgga
     5941 gtcctgggtt gaacagcgcc gctgacgtgc atcgtgtcgc tcatgactgc ccccaggcgt
     6001 cttaatggga tgaggtccgg cgctgttaag atacgtacca tccggtttta ttttcaatgc
     6061 cgggcggcga aacggagcaa gtagctggtc caggcgcgac ttgagcggta gctaactgat
     6121 gcggaaagtg aaaatacttc gagagacagg aggcgtcctg tggcgcaaca ggaccgcgcg
     6181 atccggaccc ggcgactgat tttggaggcg gccgcctcgg tctttgacga cctcggctac
     6241 gaccgcgcga ccatcgccga agtccttgag cgtgccggtg tgacgaaggg cgccctgtac
     6301 ttccacttcg cctccaagga gcagctcgcg ctcgccgtac ttgacgagca tgtgctggac
     6361 atcgcggtgg agccgcagaa gatcaagttg caggagttcg tcgactcggg ccaggtgttg
     6421 gcctaccggc tgcgcagcga cccgatccag cgcggggcgg cccggctggc cgtcgagcag
     6481 ggctccaacc acctcgaccg caagcagtcg atgctcgcct ggacccggtt cgtcgtggag
     6541 ttgctgaatc aggcgcggga ccgcggtgag gtcatggaga gcgtcgtggt gcccgagacc
     6601 gccgaactct tcgtcggtgc cttcgcgggc atccagttga tgtcgcatgc cctgaccaat
     6661 caggccgacc tcagccagcg cctgaccatc ttcttccagc acaccctccc cagcatcgcg
     6721 gtgccggcca tcctcgccaa gctcaacctg gatccggagc gcggggagaa gctcgatgcc
     6781 accctccagg ggaaggccct ggcgcacgcc ggggccgacg ccggggccga cgtcgtcacg
     6841 gggtgagtcc ggcccggccg acggctgggg ccgggtcgag tggaggcgat ccgggcggca
     6901 tcctgaggac ggcaaaaaca taccgcgcag acggtgttga atgatgaagt gtgtacgagt
     6961 catggatgac ttgtgcaggt cagaggcgtc gcggagggtt cgcggcgcct ctcgccattg
     7021 gcgggtgcgg tagtggaggc gcagccgtga gcgccggcga cgggggtggg tcgggggtct
     7081 cgccgttgcc gaacgggatt gtcagtggcg tgctgttcac tggtctcagc gggttcggtg
     7141 gagtccgcat cgagggtgag atcagaggga ggctcgatga gtggggcggg agacggcgcg
     7201 ggggagtggt gcggggtgag ggagatcacc gtcgtcgggg gcagacgcgg ggccgcgcag
     7261 cggaatagtg agcacgggta aaacgttcgt catgcacgta tcagtctggt gcgtatcggc
     7321 ccgccatccg tccacggcct gatcgtgacc gtgcccgcag acgacgcccc acgaccgaac
     7381 gcctgccgcc cgccgcttgc ttcaccgcgg cttcaccgct ggacgacacc cacccacctc
     7441 tgccgccctg gaggccgcac gcatgacccg gacgacgacg gaccagctcc ccgcacgggc
     7501 ggcggagacc gcggccaccc acccggggcc cgacccggcc gtgggcgggc ggcggagatc
     7561 ccccggcggc atcgtcccgg tcctcgcctt cgcgggcatc gtcgtcgcgg tgatgcagac
     7621 gctgctggtg ccggtcatca aagacctgcc ggtgctgctc aatacggcgc ccagcaacgc
     7681 cacatgggtg atgaccgcca ccctcctcgc cggtgccgtc tcgaccccca tcatggggcg
     7741 cctcggcgat ctgtacggca agcggcggat gctgctgacc agcctcgcgg tcatggtcgt
     7801 cggctcgctg gtctgcggct tcaccagtga tctggtcacc atgatcgtgg gccgggcgct
     7861 ccagggcttc gccatgggcg ccattcccct cggtatcggc atcatgcgtg acgagctgcc
     7921 gcgcgagcgg ctcggctcgg ccatgggcct gatgagctca tcgatcggtg tcggcggtgg
     7981 actcgccctg ccggtcgcgg ccctggtcgc ccaacacgcc gactggcaca ccctgttctt
     8041 cggtgccgcc gggctcggcg tgctgtcgat ggggctcacc ctcctcgtcg tcccggagac
     8101 ctcggtacgg gcccaggggc gcttcgacgt ggcgggcgcc ctcgggttgt cggccggtct
     8161 ggtggcgctg ctgctgcccc tcaccaaggg cagcgactgg ggctggggtt caccgaccac
     8221 cctcatcctc ttcggtgttg gggtcgtgat cctggtgctg tggggcgtga tggagctgcg
     8281 tctcgccgat ccgctggtcg acctgcggac caccgcgcgc cgcgaggtgc tgctgaccaa
     8341 cctcgcctcg ataacggtcg gtgtcgcctt ctacgccatc tctctggtgc tgccgcagct
     8401 gctccagctg cccacgtcga ccggctacgg actcggccag tcgatggtgg tggccgggct
     8461 gtgcatggcg cccctgggcc tgacgatgat gctcgtcgcc ccgctgtacg cccggatcgc
     8521 cgcccggcgc ggccccaagg tctcgctgct gctcggcatg ctcgtcatcg cgatcggcta
     8581 cggcgcgggc atcggcctga tggatgccgc ctggcagacc gtcatcatcg cggtggtcgt
     8641 cggcgccggc atcggcctgg cgtactcctc gctgcccgcc ctgatcatcg gggccgtcga
     8701 cgcctcggag accggcgcgg ccaacggcct gaacaccctg atgcgctcca tcggcacctc
     8761 ggtgtccagc gcggtgatcg gcatggtgct ggccgacatg tcgcagaaga tgggtccggc
     8821 cacggtgccc acgatggccg gcttccgggt ctcctttctc atcgccacgg ccgccgtcct
     8881 cgtcggcgtc gccctggcct gcttcctgcc gtcacagcgc aaggcggccg gcccgaccct
     8941 ggtcgccgag agcactgacg gtgcggtgga cgcggagagc gccgtcgaac tcacctccgt
     9001 ggacgggcag atggagccat tggcgaagtc ggcgccgtcc tgggcgcggc cggtcaccga
     9061 accccaccaa gccctcaccc ggcccacccc cgaggcgacg accgcgagtg tcgacgccgt
     9121 tgggttccgg ggccgggtgg cggacacctc cggcagcccg gttcccggcg cgagcatcac
     9181 cctgatcgac cggcagggcc gccaggccgg cctcgccacg gctgacgccg acggtcactt
     9241 cacactcgac gcccccacgg ccggcaccta cgtcctcacg ggcgccgcgc ccggccacac
     9301 tccgcacgcg gcctccgcca cctaccgtgg cgcgggcgcc gcgacccggg tcgacctggt
     9361 cctcaccggc accggccgac tgggcggcac catgctcggc ggcgccgagg gcaccccgct
     9421 ggccgacggg agcatcgtcg tcaccgatgc cgaaggcgcc gtggtggccc gtacggcctc
     9481 cggcgccgac ggggcctggg acatctcctc tctgccgccc ggccactaca ccgtggtcct
     9541 cagcgccgcc ggtcaccagc cgcaggcccg cgccgtggac ctgtccggcg gcgcaccgga
     9601 acggcaggac gcccgcctgc agccgacggc taccgtccgt ggcacggtgc gcggcccgca
     9661 cggtcggccg gtcgccgacg ccgccgtgac cctcgtcgag gacggcacgg tcgccgggca
     9721 caccgtcacc ggaccggacg gcgccttcgc cttctccggc ctgagcggcg ccgactacac
     9781 cctcacggcg gccggttacc cgccacacac cgccccgata tccctcaccg ccggcaccca
     9841 cgaaaccctg gacctggaac tggacctggc acagccaccg atacccggcg gctgacggac
     9901 tccggcacgg ggccgcaggc tcgcagacgc acacccgcag acacatgcag gcgccctgcc
     9961 gagtgccact cacccggcag ggcggcggct tctcagccgc ggtcccaagt cgactgctcc
    10021 gcctgcgacg tgacccacga atctagccgt ccgccatcca tcccgccgtg ccgcgggatg
    10081 cttcagcgct tcggcgggtc cggagggaga aagtcgtcga acgtcggtgt gccgggttcc
    10141 ggggcgagtc tggtggcgcg ggcggtgaag ctgatggcgt ggtaccagcc gcacagcatc
    10201 agcaggtcca ggagctgttc cgggctgaac tccgcgctga ggcgctccca tagcgtgtca
    10261 tcgatgtcgt ggccggtgtg cagggcgtcg gccgcgtcga ggaggagtcg gtcgcggtcg
    10321 gtcgtccagc acgtgtccgc gctgctgccg tgcgtcaggg aggtgatctg cgccggggtc
    10381 agtccggcgc ggggggcgaa gcgcgcgata tgcacgcccc attcgtacgc gcagccgcag
    10441 cgtgcgcagg tgcggtcgat gaggatctcg cggtcccgca gaccgaggct gagtcgccgg
    10501 ctgagctggt acgagcccca ggcgtgcagc gcctcggtca gcgggaggtt gcgtgcgtag
    10561 agccggaaga gcgcgatcgg ctcctcgccc cccggcatca tgcggtgcag tacttccgcc
    10621 acctctgacg ggtgcggcgc ctcggtcggt gcaatccggg caggcatgac tccccttccg
    10681 cttgatgtgc ttttcgaagc agcggaagga ggctagtgct tctgaaatcg aagcgctagg
    10741 ggtgccggtg cgcccctcag gcctggtact ccgtgagatc gatgctgtgc accgtcagcg
    10801 catggccgta ctccggatgt gtggcctccc gttcgaaggt catgccgagc ttgcgtacga
    10861 cgttcaggga ggactcgtgg ccgagcgggt tgacggcgat gacgcggtcg aggccgcggt
    10921 cctggagggc gaactccaag acggcctggg cggcttcgga ggcgtagccc tggccccagt
    10981 actgcgggcc caggcgccag ctgatcgcca cctcgtgttc ggcctccggg aggaacgcgg
    11041 g
//
