LOCUS       NZ_CP050976            11935 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8102879
            Orig. end                         :: 8114814
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="33"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            739..1116
                     /locus_tag="HEP86_RS35845"
                     /old_locus_tag="HEP86_35845"
     CDS             739..1116
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057039.1"
                     /locus_tag="HEP86_RS35845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35845"
                     /product="DUF2267 domain-containing protein"
                     /protein_id="WP_168482754.1"
                     /transl_table=11
                     /translation="MDDKEFFRAVADRARLSRQEGADLTRATLETLALRLSDGEARDLA
                     LELPEPLRVSLKRERREMEIFGPDESIRRVRARTGLSASEADRGVRAVLGTLQEAISRK
                     EFGHAMSQLGKEYAQLVETTR"
     misc_feature    complement(1308..1322)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.7"
                     /tool="antismash"
     misc_feature    1397..1411
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.1"
                     /tool="antismash"
     gene            1622..2893
                     /locus_tag="HEP86_RS35850"
                     /old_locus_tag="HEP86_35850"
     CDS             1622..2893
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="HEP86_RS35850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35850"
                     /product="MFS transporter"
                     /protein_id="WP_168482755.1"
                     /transl_table=11
                     /translation="MTGTAPAVAPAAPQPHVRRDIRLFWWAGTCDALGSQTSGLVLPLL
                     LLTLGRSPAEVGALAGLSAVATLLLGPFAAVPADRGARKHLMVGSALVSAAAMGGVTAA
                     VAAGPVPLAVLIAAVLVERCATSCYEAAALGTVALVCPPGEYRRVLSRLQAGERGALVL
                     GPALGGLLFHAERWLPFLADALSYAVAAGCICAMRAPLAPPREPQSPAAPVRRGRRALV
                     AEAAAGLALIRREPFLRLALLWISAVNALLVALYYTTVFALQHDGRGAAPLGLVLALAG
                     AAGLAGALAAPRIAGRRSAGRVLVTVSWLMVPPAAALALARDAWHFGLLFGLLCLLTPV
                     ATVALQARIIQVIPPGLQARTGTVLATASGATAAVAPALAGLSADRAGAAVTLMGCAAL
                     LAVLALHTTCVAPSRTRCTGSEPA"
     misc_feature    2078..2080
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2890..4740
                     /locus_tag="HEP86_RS35855"
                     /old_locus_tag="HEP86_35855"
     CDS             2890..4740
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088741.1"
                     /locus_tag="HEP86_RS35855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35855"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_168482756.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 5.6e-35, bitscore:
                     119.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTTAHLTAQPPAGTGFRVYQPALPEVCPRDPARMALAADADGR
                     CEIFTWNAATGTARQVTDSPHGTLHCALDADARVWWFAEDRHGHGLWYFQDFEGGARQP
                     GLTGLAPGRPHGLAVSDAGTVALGLGDGSGMTVHLGRPGGPARQVWTVDGQALLTGISP
                     SGGLLALSGAAGSDRAVTLVTCSGKVLDQLSGHHGRLWALGFAPSPAGTELLLVQEHDN
                     RYRLATWRPGSGLLPQDWCRFDTEITARWYPAGRRVLIRQDRHGRSTLAAADLDRRTLT
                     PIPSPPGTLLDAAPHPGDEVHYLWTDTATPPRMCSTAGTRLPELATLPAPAPGRHRELW
                     TPGPDGPVHTLLSEPEPVTGRRPPLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVN
                     YRGSTGYGPRWRAAYGAGVGLTQVADLVAVRGDLLRRGLARADAIGLWGTSWGGYLALL
                     AMGTRPDLWQAGVAVKPVADCAAAYRTGTPALRALDERLFGGTPDTVPERYARSSPLHY
                     VADVRAPLLVIAATLDTKCPPGQVRSYLGALRRAGAAHDSMWLETGHDGYTGAHHVAVL
                     HRALGFLDQRLRRAPAAHRTPRPEVDGVSGGTGPSGAPGA"
     gene            5001..6935
                     /locus_tag="HEP86_RS35860"
                     /old_locus_tag="HEP86_35860"
     CDS             5001..6935
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088743.1"
                     /locus_tag="HEP86_RS35860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35860"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_168482757.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.1e-183, bitscore:
                     610.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGRADVLHANLEFT
                     DWITRRTEFTADDYQYYALSSYFMGCGDWVFSSALYDDPEWRVPEFTASMRGKLRDERL
                     RMSKELHRVVPEFVQETAERIVAAGPDVVGFTSTFQQNTAALAAIKYVKRLAPHIRTVM
                     GGANCDAEQGHATHRNFPFVDFVVRGEGEAAFPQLLTALDEGGDLSAVPGLCHRGADGE
                     SIANPMSTRPLPPATILPPDYRGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPETFYDEIMELARRHRVLDMYLVDNILDMGYLTTVLPRIIDSGYD
                     LRMHIEIKANMRRTQLRTLAQAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVSWNYLHGFPGESATDYEPVIAQIPALEHLDPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDMAYVFEAPERGITKPTVAALNEALAAWKKRHADSR
                     LTHADLGDRIVLVSRRHAFGWGALELTAPTEIAAFRLLDQPHAPAALTRKLAAKLPGHT
                     VDEAAVHALLQHWVTLGLVFTDGGQYVHLAPAAVNEDLLRLDFMRHTHAAAAAPERPDD
                     AVRALVAHV"
     gene            6983..7657
                     /locus_tag="HEP86_RS35865"
                     /old_locus_tag="HEP86_35865"
     CDS             6983..7657
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="HEP86_RS35865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35865"
                     /product="DUF5825 family protein"
                     /protein_id="WP_168482758.1"
                     /transl_table=11
                     /translation="MTSTATPPARALTLHAWRDYDQDACALPGMSLGAVDLTAPPDGQA
                     ARLWELGARRVEFTGEIDLTAVDDPADAARAVRSLCLIRDLTARAVLVQWQLRLPPEPD
                     DGWRDLSHLQPPRTLSGPTDPAAALAEWRNGHYLCKCLWRQGPGFVQIRDRRWGELRRF
                     TAEEPEYQQAIDRLSYGAPLRDVPPAIAADFLAEHLVSRTGPLLWWLPYRVNRWIQEAM
                     AV"
     gene            complement(7707..8765)
                     /locus_tag="HEP86_RS35870"
                     /old_locus_tag="HEP86_35870"
     CDS             complement(7707..8765)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /locus_tag="HEP86_RS35870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35870"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_168482759.1"
                     /transl_table=11
                     /translation="MRPTGHSGLRRTLTVFIAVLAVALGLGAQTGTAQADDGSGLNGLT
                     FTSVNNGRNLDVQNGNTGDGVFIVTNSAPGYHQKWNASLQADGSFTLVNDVTGKCVGAG
                     YPLKQQSCSGSSGLRWYFQPVSGTSDTFMIRNAGDNKCVDVVLGAQYNDAWTQTYGCNG
                     SKAQQWKIPSSATGAVFKAAVAYASKRCQKDAATCSWTKGSQAPAEPLPKQCVSPVWYN
                     GTEAPVPWTFSLNTSTGWSSQLGVSFTSTLGTGMASPVQTSVSLTLSGQVTYDLRTDLG
                     NSLVITVPSHQYGWVALSELATKVTGEWTFDANGYPWKAQDTVTVPLRSDDQGRSSIYL
                     AQTSPDFTNCNS"
     misc_feature    complement(8739..8741)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    8799..8814
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.01"
                     /tool="antismash"
     gene            complement(8857..9852)
                     /locus_tag="HEP86_RS35875"
                     /old_locus_tag="HEP86_35875"
     CDS             complement(8857..9852)
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088746.1"
                     /locus_tag="HEP86_RS35875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35875"
                     /product="DMT family transporter"
                     /protein_id="WP_248001469.1"
                     /transl_table=11
                     /translation="MGTAAVDGDAGSVSRADGRALAAAGVTVVLWASSFIAIRSSAADF
                     GPGALALGRLVVASVVLSALLLARRGGFPPREAWPGILASGVLWFGAYMVALNWGERHV
                     DAGTASLVVNVGPILMALLGGWLLKEGFPPKLMAGMAVSFAGAVVVGLSMSGGGTSSLV
                     GVLLCLFAALAYAVGVVLQKPTLKHATPMQVTTFGALAGAVACLPFAGQLVSQVSRAPL
                     SATLQIVYLGVFPTALAFTTWAYAVSRTTVGKMGATTYAVPVIVVFLSWLLLGEIPGWL
                     TLLGGLLCLSGVAVSRMRGKGRAQVPEAARGSRTAAQAEVSAECAQRTAG"
     gene            complement(10004..10639)
                     /locus_tag="HEP86_RS35880"
                     /old_locus_tag="HEP86_35880"
     CDS             complement(10004..10639)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035487.1"
                     /locus_tag="HEP86_RS35880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_35880"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_168482760.1"
                     /transl_table=11
                     /translation="MTRMPITNRNNTTNTPMTAAIDDAPLISRVLADAFDDDPMMRWFF
                     PDDAARPAGLVRYFSTLFTRQYGRHGVCERTGAAAAFWVAPEAMAKAVPDAETVEELQD
                     ILGDRAALFREAVEAAAEHAPQEPQWHLAVLGADPAAQGQGHGAALLRSGLAKADAAGM
                     PVALESSKRTNLPFYEHFGFTVRQELQLPGGGPTLWAMRREPRPSGDA"
ORIGIN
        1 tatctgttca cttccgccgg tggaaagaag gaccgcggcg ccggtctgct gtccggtgag
       61 ggattcggcg gcggtgagtc gcccgacgtc atcaccgtgc gcaccgcgta cccctatcag
      121 accctcctcg cggttcccga gaagtccgac ctcccgtcct tctccctctg ggcggcctac
      181 gtcccggagg acacggagca gcgcaccaat cacgcctttg gcctgctcat gatcgcgaag
      241 ccgccgatcc cgggagccct gcatctcgcc tggccgctga tccggcgctt caccgagcgg
      301 gtgttcgccg aggaccggat ggcggtcgag gccgagcagc gggcctggga cgagcagggc
      361 gaggactgga accaggaggt cttcccgctg atcctcgacg tccgtgacgt gctgcgcacc
      421 aacggggtcc cgatccgccc cgaatccgcc ctctgcggcg ccgccgcgct gtgcgacggc
      481 gcgccgcggg ccggcgccgc ctcggaggcc ggatccccgc agccgggctg accggcccgg
      541 ctcgtggtgg cggctgcccc cgctcgcggc gctgtcgtct cgaccggcag cgccgcaggt
      601 cagacggcct ccggcggtga cgttcggtgg gtgcgcgggt gcagccggtg gtgggccgac
      661 ggtgggcggg ggatcgtggc aaggtggtgg gtgaggtgcc tccctcgccc atcccctgat
      721 tccgatgtgg aggctcgcat ggacgacaag gagttcttcc gggcagtggc ggaccgcgcg
      781 aggctctcgc ggcaggaagg ggcagatctc acccgcgcca ccctcgaaac gctcgcgctg
      841 cgtctgagcg acggcgaagc ccgcgatctg gccctcgaac ttcccgagcc gctgcgtgtc
      901 tcgctgaagc gggagcggcg ggagatggag atcttcgggc cggacgagtc gatccgcagg
      961 gtacgagcac gcaccggcct gtccgcgtcg gaagccgacc gcggtgtacg cgcggttctc
     1021 ggcacgctcc aagaggccat ctcccgcaag gagttcggac acgccatgtc ccaactcggc
     1081 aaggagtacg cgcagttggt ggaaaccacg cgctgatgta gccgccttct cggtgccgtg
     1141 gtcccgtcgc cgcctacgga gtcgtcgccc tgctccgcgg cgaatggtcc gcggcccgaa
     1201 cggtgtggcc ggactccacg agggctccac gatcgcgcga acggccgggc ggtcgttgcc
     1261 tcttcgggct acgacctgcc cggccgcgcc gcgcgaagct gttcctcgcg cacaccttgt
     1321 ggcccacgtc actgtctggc taagctccgg cacgattcgg gcacatttcc tccgtaagtt
     1381 gacctattgg tgtgcctgat cttcatgatc tgcccacatg tgtgaggtgg cttcgcatgg
     1441 aacggtcaac ggcatccccg gcagacggcg caccctccga cttaccgtgc gccgcctccc
     1501 gggccgggac agcggacggc ctgacacgcg cccgcaccag cggccgtacg caccgcccgg
     1561 cacggacccc gcacggcgat gcccaccacg gcactgcgag accgtccccg ggccgttcct
     1621 gatgaccggg accgcgccgg cggtggcacc ggccgcaccg cagccccatg tccgccgcga
     1681 tatccggctg ttctggtggg cgggcacctg cgacgccctg ggcagccaga catccgggct
     1741 ggtcctgccc ctgttgctgc tgaccctcgg ccggtcaccc gccgaggtcg gcgccctcgc
     1801 cggactgtcc gcggtcgcga cgctgctgct cggcccgttc gccgccgtcc ccgccgaccg
     1861 cggggcgcgc aagcatctga tggtgggctc cgcactggtc tccgccgccg cgatgggcgg
     1921 ggtcaccgcc gccgtcgcgg cgggcccggt cccgctggcc gtgctcatcg ccgccgtgct
     1981 cgtcgagcgg tgcgcgacct cctgttacga ggccgcggcg ctcggcaccg tcgccctggt
     2041 gtgcccgccc ggggagtacc ggcgcgtcct gtcccgatta caggcggggg agcggggcgc
     2101 cctggtgctc gggcccgcac tgggcggtct gctcttccac gccgaacgct ggctgccgtt
     2161 cctcgcggac gccctctcct atgccgtcgc ggcgggctgt atctgtgcca tgcgggcgcc
     2221 cctcgctccg ccccgtgagc cgcagtctcc ggccgcaccg gtgcgccgcg gccgacgggc
     2281 cctggtcgcc gaagccgcag ccgggctggc gctgatccgc cgtgaaccct tcctgcggct
     2341 ggcgctcctg tggatctcgg ctgtcaacgc cctgctcgtc gcgctgtatt acacgacggt
     2401 gttcgccctg cagcacgacg gccggggtgc cgcaccgctg ggactggtcc tcgcgctcgc
     2461 cggggcggcc ggtctggccg gagcgctcgc ggcaccccgg atcgccggcc ggcgctccgc
     2521 gggccgggtg ctggtgacgg tttcctggct gatggtgccg cccgccgcgg ccctcgcgtt
     2581 ggcccgcgac gcctggcact tcggcctgct tttcggtctg ctgtgtctgc tgacgccggt
     2641 ggccaccgtg gcgctccagg cccggatcat ccaggtcatc ccacccggac tccaggcccg
     2701 caccgggacc gtgctggcca ctgcgtcggg cgcgaccgcc gccgtggcac ccgccctggc
     2761 cgggctgtcg gccgaccggg ccggagccgc cgtcaccctg atgggctgcg ccgcactgct
     2821 ggccgtgctc gcgctccaca cgacctgcgt ggcaccgtcc cgcacccgct gcaccggttc
     2881 ggagcccgca tgagcacgac cacggcacac ctcaccgcac agcccccggc cggcaccggc
     2941 ttccgggtct accagccggc gctgcccgag gtctgtccac gcgacccggc gcggatggcg
     3001 ctggccgcgg acgccgacgg ccgctgcgag atcttcacct ggaacgccgc caccggcacc
     3061 gcccgccagg tcactgacag cccgcacggc accctgcact gcgccctcga cgcggacgcc
     3121 cgggtgtggt ggttcgcgga ggaccgtcac ggccacggac tctggtactt ccaggacttc
     3181 gagggcggag cgcggcagcc gggcctgacc ggcctggcac cgggccgccc gcacggcctc
     3241 gccgtcagcg acgccgggac ggtggccctc ggcctagggg acggcagcgg aatgaccgtc
     3301 cacctcggga gaccgggcgg cccggcccgg caggtgtgga ccgtcgacgg gcaggcgctg
     3361 ctgacgggca tctcaccctc gggagggctg ctggccctgt ccggggcggc cggctccgac
     3421 cgggcggtca cgctcgtgac ctgctccggg aaggtgctcg accagctctc cggacaccac
     3481 ggccggctct gggcgctcgg cttcgcaccg tccccggcgg gcaccgagct gctgctggtc
     3541 caggagcacg acaaccgcta ccggctggcg acatggcggc ccggcagcgg actgctgccg
     3601 caggactggt gccgcttcga caccgagatc accgcacgct ggtaccccgc gggacgccgc
     3661 gtcctcatcc gccaggaccg gcacggccgt tccaccctcg ccgcggccga cctcgaccgg
     3721 cgcaccctga ccccgatacc gtcgccgccc ggcaccctgc tcgacgccgc cccgcacccg
     3781 ggcgacgagg tgcactacct ctggaccgac accgccaccc cgccccggat gtgctccacc
     3841 gccggcaccc ggctcccgga actcgccacc ctgcccgccc ccgcacccgg ccggcaccgc
     3901 gaactgtgga cccccggccc ggacggcccc gtgcacaccc tgctcagcga accggaaccg
     3961 gtcaccggcc gccggccccc gctggtcttc ctggtgcacg gcggccccgc cgaccacgac
     4021 cgggacgcct acgacccgga ggtgcactcc ctggtcgctt ccgggttcgc cgtcgcccgg
     4081 gtcaactacc gcggctccac cggctacggc ccccgctggc gcgccgcgta cggcgcgggc
     4141 gtcgggctca cccaggtcgc cgacctcgtc gcggtccgcg gcgatctgct ccgccgcggc
     4201 ctcgcccgcg cggacgcgat cggcctgtgg ggcacctcct ggggcggcta tctggccctg
     4261 ctcgcaatgg gcacccggcc ggacctctgg caggcgggcg tcgccgtcaa acccgtcgcc
     4321 gactgcgcgg cggcgtaccg caccggcacc cccgccctgc gcgccctgga cgagcggctg
     4381 ttcggcggca cgccggacac ggtgcccgag cgctacgccc ggagctcccc cctccactac
     4441 gtcgccgacg tccgcgcccc gctgctggtg atcgccgcca ccctcgacac caaatgcccg
     4501 cccggccagg tacggagcta cctcggcgcc ctgcggcggg ccggtgcggc acacgattcg
     4561 atgtggctgg agaccggcca cgacggctac accggcgccc accacgtggc cgtactccac
     4621 cgggcgctgg gcttcctgga ccagcgtctg cgccgcgctc ccgcggccca ccgaaccccc
     4681 cgtcccgagg tggacggggt ctccggaggc accggcccgt ccggtgcccc cggggcgtaa
     4741 gaaccgttcc gcaaccggca gaagaggaga acagccatgc agaaggacat catccacaac
     4801 gacccgctcg cgggtgacga ggagaaccgc agaccgagca tcggcgtcac ggtgaccgtc
     4861 ccgttccgta acgccgagga caccgaggag gactgaccct ccccggccgg cccggtggca
     4921 caccgggccg gccggaccgc cgcacctcac cggccggccg tcggccacct cccaccccac
     4981 ccgtcgtacg aggagccgcc atgcgcgtcc tgctggtcaa catgccctgg tccccgatcg
     5041 acctgccgtc cctcgccctc ggcatcctga gacgaagcgt cgacgagcgc acctccggcc
     5101 gggccgatgt cctgcacgcg aacctggagt tcaccgactg gatcacccgg cggaccgagt
     5161 tcaccgcgga cgactaccag tactacgcac tctcctccta cttcatggga tgcggcgact
     5221 gggtcttctc ctccgccctc tacgacgacc ccgagtggcg ggtgccggag ttcaccgcct
     5281 cgatgcgcgg caaactgcgc gacgagcggc tgcggatgtc caaggaactg caccgcgtgg
     5341 taccggagtt cgtgcaggag accgccgagc ggatcgtcgc ggccggcccc gacgtcgtcg
     5401 gcttcacctc caccttccag cagaacaccg ccgcgctcgc cgccatcaag tacgtcaaac
     5461 gcctcgcccc gcacatcaga accgtcatgg gcggcgccaa ctgtgacgcc gagcagggtc
     5521 acgccaccca ccgcaacttc ccgttcgtgg acttcgtggt ccggggcgag ggcgaggccg
     5581 ccttccccca gctgctcacc gcgctcgacg agggcggtga cctgtccgcc gtccccgggc
     5641 tgtgccaccg cggtgccgac ggcgaaagca tcgcgaaccc gatgagcacc cggccgctgc
     5701 cgcccgcgac catcctgccg cccgactacc gcggctactt cgagcggctg gcgtcctccg
     5761 tcgcccgcaa ctgggtggag cccaaacttg tcgtcgaggg ggcccgcggc tgctggtggg
     5821 gggagaagca ccactgcacc ttctgcggtc tcaacggctc cttcatgcaa ttccgcagca
     5881 agagccccga gaccttctac gacgagatca tggagctggc ccgccggcac cgggtgctgg
     5941 acatgtacct cgtcgacaac atcctcgaca tgggctacct caccaccgtc ctgccccgca
     6001 tcatcgacag cggctacgac ctgcggatgc atatcgagat caaggccaat atgcgccgga
     6061 cccagctgcg caccctcgcc caggccggga tgatctacgt ccagccgggc atcgagagcc
     6121 tcaacagccg ggtgctggac ctgatggaca agggcgtcag cggctgccag aacgtccgca
     6181 tgctccggga cggcgccgag accggactct ccgtctcctg gaactacctc cacggcttcc
     6241 ccggcgagag cgccaccgac tacgagcccg tcatcgccca gataccggcc ctggaacacc
     6301 tcgacccgcc ggtcgacctg tccgcccgta tcgccatcga acgcttcagc ccctacttca
     6361 accggcccga actcggcttc accggtctgc gccccgagga gcactaccgc ttcacctacg
     6421 acctgcccga atccgaactc ctcgatatgg cctatgtctt cgaggcaccc gaacgcggga
     6481 tcaccaagcc caccgtcgcc gcgctcaacg aggccctcgc cgcctggaag aagcgccacg
     6541 cggacagcag actcacccac gcggacctcg gcgaccgcat cgtgctcgtc agccgccggc
     6601 acgccttcgg ctggggcgcc ctggaactca ccgcacccac cgagatcgcc gccttccggc
     6661 tgctcgacca gccgcacgcc cccgccgccc tgacccgcaa gctcgccgcc aagcttcccg
     6721 ggcacaccgt cgacgaggcc gccgtccatg ccctcctcca gcactgggtg acgctcgggc
     6781 tggtcttcac cgacggcggc cagtacgtcc acctggcacc ggccgccgtc aacgaggacc
     6841 tgctgcgcct cgacttcatg cgccacaccc atgccgcggc ggccgcgccg gagcgccccg
     6901 acgacgccgt ccgggctctc gtcgcccacg tctgaccccg cccggcccgc agcaggactc
     6961 acatgccccg caggagaacg ccatgaccag caccgccacc ccgcccgccc gagcactgac
     7021 cctccacgcc tggcgggact acgaccagga cgcctgcgcc ctgccgggca tgagcctggg
     7081 cgcggtcgac ctgaccgcac caccggacgg ccaggccgcc cggctgtggg aactcggggc
     7141 acgccgggtg gagttcaccg gggagatcga cctgaccgcg gtcgacgacc ccgccgacgc
     7201 cgcccgggcc gtccgcagcc tctgcctgat ccgcgatctg accgcccggg ccgtactggt
     7261 gcagtggcag ctgcggctgc ccccggagcc cgacgacggc tggcgcgacc tcagccacct
     7321 ccagccaccc cgtaccctca gcggccccac cgaccccgcc gccgcgctgg ccgagtggcg
     7381 caacgggcac tatctctgca agtgcctgtg gcgccagggc cccggcttcg tccagatccg
     7441 cgaccgccgc tggggcgaac tgcgccgctt caccgccgag gagcccgaat accagcaggc
     7501 gatcgaccgg ctctcctacg gcgcaccgct ccgggacgtg cccccggcca tcgccgccga
     7561 cttcctggcg gagcacctgg tctcccgcac cggcccgctg ctgtggtggc tcccgtaccg
     7621 ggtgaaccgg tggatccagg aggcgatggc cgtctgagga ccgtagggcg ggcccgcacg
     7681 acccgtgcgg gcccacccct tctgattcag ctgttgcagt tcgtgaagtc cgggctggtc
     7741 tgcgccagat agatgctgga ccggccctgg tcatcgctgc gcagcgggac cgtgacggtg
     7801 tcctgcgcct tccacgggta gccgttcgca tcgaaggtcc actcgccggt caccttcgtc
     7861 gccagctccg acagcgccac ccagccgtac tgatgcgacg gcacggtgat caccaggctg
     7921 ttgccgaggt ccgtccgcag gtcgtaggtg acctggccgg agagggtgag gctgacgctg
     7981 gtctgcacgg ggctcgccat gcctgtgccg agggtggagg tgaaggacac tccgagctga
     8041 ctcgaccagc cggtggaggt gttcagcgag aacgtccagg gcaccggcgc ctcggtgccg
     8101 ttgtaccaaa cgggagagac gcactgcttc ggaagcggct cggccggggc ctgcgacccc
     8161 ttcgtccagg agcaggtcgc ggcgtccttc tggcagcgct tcgacgcata ggccaccgcg
     8221 gccttgaaca ctgcgccggt cgccgaggag gggatcttcc actgctgcgc cttgctgccg
     8281 ttgcagccgt aggtctgggt ccaggcgtcg ttgtactggg cgcccagcac gacatcgacg
     8341 cacttgttgt caccggcgtt acggatcata aaagtgtcgc tggtgccgct cacgggctgg
     8401 aagtaccagc gcagaccgga cgatccggaa caggactgct gcttgagcgg atagcccgcc
     8461 ccgacgcact tgcccgtgac gtcattgacg agggtgaacg acccatcggc ctgaaggctg
     8521 gcgttccact tctggtggta gcccggcgcg gagttggtga cgatgaagac accgtccccg
     8581 gtgttgccgt tctgcacatc caggttccgg ccgttgttga ccgacgtgaa cgtcagtcca
     8641 ttcaggcccg aaccgtcatc ggcctgagcc gttccggtct gcgcacccag gccgagggcg
     8701 acggcgagca cggcgataaa gacggtgagt gttctgcgta agccgctgtg tccggtcggt
     8761 ctcataccag aacaaccccc ttatccattc ggagtattgg tggtgtagtc caccgtgctc
     8821 cgcacgctat cgcccccgcc gccccggcgg cagtgctcag ccggcagtgc gctgtgcgca
     8881 ctccgccgac acctcggcct gcgcagccgt acgactcccg cgtgcggcct ccggtacctg
     8941 cgcccgcccc ttcccccgca tccgcgacac cgccaccccg gacagacaga ggaggccacc
     9001 cagcagcgtc agccagccgg ggatctcgcc gaggagcagc caggacagga acacgacgat
     9061 caccggtacc gcgtacgtcg tggcacccat cttcccgacg gtcgtacggg acaccgcata
     9121 ggcccacgtg gtgaacgcaa gggccgtcgg gaacacaccc aggtacacga tctgcaaggt
     9181 cgccgacagc ggagcccggc tcacctgcga caccaactgg ccggcgaacg gcagacaggc
     9241 caccgctccg gcgagcgcgc cgaacgtcgt cacctgcatc ggtgtggcgt gcttcagggt
     9301 cggcttctga agcaccacac ccaccgcgta cgccagcgcc gcgaacagac acagcagcac
     9361 gccgacgaga gacgacgtgc caccgccgga catggacagc ccgaccacca cggccccggc
     9421 gaacgacacc gccatacccg ccatcagctt cggcgggaag ccctctttca gcagccatcc
     9481 gcccagcagc gccatcagga tcggcccgac attgacgacc agggaagccg taccggcgtc
     9541 cacatgccgc tcgccccagt tcaacgcgac catgtacgcg ccgaaccaca gcactcccga
     9601 ggcgaggatg cccggccagg cctcgcgggg cgggaatcca ccacgccggg ccagcaagag
     9661 ggcactcaac acgacggacg ccaccaccag ccggcccagc gcgagcgcac ccggcccgaa
     9721 atccgcagcg gaactacgga tcgcgatgaa cgacgacgcc cacagcacca ccgtgacgcc
     9781 ggccgcggcg agcgcacgcc cgtccgcccg gctcacggac ccggcatcgc catccaccgc
     9841 tgctgttccc acccgccgac tcactcctgc gacctcccca agcccgttgc gtacgcccgg
     9901 cgcgcacaca tgagccgcca gcttgtaaga ggccgcaccg cactgacaag cgaataccgg
     9961 tcttgcccga gccatgccga ctcgcgcagc ctccggcctg accctaggcg tcgcccgacg
    10021 gacgcggctc ccgtcgcatc gcccacagcg tcggcccgcc gcccggcagc tggagctcct
    10081 gccgcacggt gaacccgaag tgctcgtaga acggaagatt ggtccgcttc gacgactcca
    10141 gagcgacggg catacccgcc gcgtcggcct tggccagccc cgaccgcagc agggcggccc
    10201 cgtgcccttg gccctgggcg gcggggtcgg cccccagcac cgccagatgc cactgcggct
    10261 cctgcggggc gtgctcggcc gccgcttcga cggcctcccg gaacagcgca gcccggtcgc
    10321 ccaggatgtc ctgaagctcc tcgacggtct ccgcatcggg cacggccttg gccatcgctt
    10381 ccggcgccac ccagaacgca gctgccgccc cggtacgttc acacacaccg tgccgcccgt
    10441 actgccgggt gaacagggtg ctgaagtaac ggacgagccc cgccggacga gcggcatcgt
    10501 cgggaaagaa ccagcgcatc atcgggtcgt cgtcaaaggc atcggcgagg acccggctga
    10561 tgagcggagc gtcgtcgatc gcggccgtca tcggggtgtt tgtggtgttg ttccggttgg
    10621 ttatcggcat acgggtcatt ctgcacgctc gatgatcttg agttgcaggg ggatcggttg
    10681 tggcgtagac ggcgacggcg ccccggccca ccccctgatc accggcctgg cctcggctgc
    10741 cgtggaccgt gtcggcgcgc ggtaagcgca gcaggccgcc gactcaagtg gagccggtcc
    10801 ctcccgggac ggacccggac actcccggat gagttccggc cattgggcgg cggaatcgtc
    10861 ccgaccggtc aggggtgcgg taggaaaggg gccggttcgc ggaaacctcc catccaggga
    10921 ggttgaggga gttctcggta cgtcacggac gggtgcgtgg catgcccaag gcggtgcagg
    10981 gcggtgcggg gagggcaacc gctccgcgcg gccgtgaggt gaggtgatcg gcccccgcct
    11041 ctcccgtggg gtaaggcgat cggccccgcc tctcccgtga gcgcaccgaa cgaacagctc
    11101 ccctccggcg ttgccgggtc cttcccgtcg tgccccaccg gctcaatccg gtgccgacgg
    11161 gggccattcc cgtgttctcg gaaacggaga ccatgatgcg aagacctatc cgtgccgcgg
    11221 tggcggtggt cgcgaccgct ttcctcatac ctctgctgac ggcgagtccg gcgcccgcct
    11281 tgtcgggccg gcccgccgac ggcgcccgta agcccgccga ttgtcggccc gccctccagg
    11341 tcctggcctc tctgccgggc tcaggtggca gccaggtcaa gggacttggg ccgactgccc
    11401 tggcggtggg ggtatcgcgc gggttgcccg tgtactggac gggggcccgg gtccatcgcg
    11461 taccgctgcc cgccggattc accggcggtg aggtcgcggc ggtcaacgcg aaggggctga
    11521 tggtcggttc gctgcacgcc gcgggccgta ccgccgcctt cagtttccgc gccggcgccc
    11581 gcgcggtgac cctgctgccg gaaggggcgc atgcggcgga cgtcagcgac caggggctga
    11641 tcgtcggtga cgcggtcaag ggcggctctc tcgtcggcct cgaatgggag ggcgcgcgga
    11701 tcctgcgtcg gctcgtgcca ccggccgggt actcgctgac ttcggtgacc gccctcaaca
    11761 acgccggtca gatcgccggt tccggtgagg ccgtcaagga cgacgagtcg tggtcggcgg
    11821 gcctcgtctg gcccgccagg aggagcgctg cggccattcc gctccaacgg ttctggcccg
    11881 cgggagtgcc ctacgacttc tggtaccccc gcgatatcga ccgcgccgga cgcat
//
