LOCUS       NZ_CP050976            22542 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7636782
            Orig. end                         :: 7659324
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22542
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="29"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22542
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22542
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12542](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12542)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(250..270)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.57"
                     /tool="antismash"
     misc_feature    complement(582..596)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.62"
                     /tool="antismash"
     misc_feature    complement(582..597)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.09"
                     /tool="antismash"
     misc_feature    1281..1294
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.25"
                     /tool="antismash"
     misc_feature    complement(1913..1927)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            complement(3835..7011)
                     /locus_tag="HEP86_RS33950"
                     /old_locus_tag="HEP86_33950"
     CDS             complement(3835..7011)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088776.1"
                     /locus_tag="HEP86_RS33950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33950"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_168482474.1"
                     /transl_table=11
                     /translation="MTTAMQPEVPEPGDADPAHPPYPTGAKPVLAPGAQVRIRDEQWLV
                     KSLGESRDGLMVEVSGVSPFVRGTDAVFYSGLDQIDVLDPRKTRLVPDGTSKHAKARLY
                     LEAVIRKTALPQTEHGIALAGSFLMDRQEHQLRPAELALSMRNPQPRLLIADVVGLGKT
                     LEIGVTLSELIRRGRGERILVVTPAHVLEQFQRELWTRFALPLVRLDSTGIQRIQQEIP
                     AGRNPFAHFKRVIVSVDTLKSATYAHHLEKITWDAVVIDESHNLVNKGTRNNRLARRLA
                     EQTDALILASATPHNGDAESFAELIGMLDPAAIADPKNYKVAALDHLYIRRTKSDREVR
                     DGLKDKPWAERGESLPVPAPATRKEVAVLEKLASEWTPGEPNRSSVCADPLVGYNFLKA
                     FLSSHVALRKTLTARRAYLDNPRSAMVKGKDGKKADSPERRAALAAEGAALAELEALVA
                     DFTDQDSAKLDALVRTLKDDLQVGPGCERRVVIFSERVHTLDWLAQEVPALLGFKKKPA
                     AKADKTRPWQAYGGVVEVMHGDTTNDQQQREIIDRFGRADEPVRLLFTGDIASEGVNLH
                     HQCHDLVHYDLPWSLIRIEQRNGRIDRYGQAVSPEFRAITLTADVPWRRDEESGRTLTL
                     DDRLVGARLLRREAQAHEIETGEGSAEAVTGLYDDKKEEDRLTRDLIKGGTVERSIKQS
                     QQESGGVLADLLAGANARLADPTAAPATLGTGSVPEADVPHVFADTKAYFHAAMDLIYP
                     QAERKALDWKPETAHGRIEFTPPGDLQYRFRELPKSYLEQEKILTTPKYDGTLRLTFDK
                     RYAADRLDAARNAKQGKTDQPTSQWPNVSYVSDIHPVLDWVTDKVLAKLRYDEAFVLAH
                     QPDADKAKRIDAALPEALTSPVYLLQGLYSNAAGKPTVVEWMAVTGLAEAAPRVWRMDS
                     AFLAACGVGPGMPGRGRPVDPDLLQALVPAAVDAAETHLRERRVDYDKQVDSYLAPYED
                     RVQVWEQGALIAVGNQQQRRKQVYDTAARRRKLVRRLRTDGDPMLRVLCVLEPLHTTTV
                     SAAHAEESAR"
     misc_feature    complement(6922..6924)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7176..8384)
                     /locus_tag="HEP86_RS33955"
                     /old_locus_tag="HEP86_33955"
     CDS             complement(7176..8384)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088777.1"
                     /locus_tag="HEP86_RS33955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33955"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_168482475.1"
                     /sec_met_domain="Pkinase (E-value: 2e-37, bitscore: 127.8,
                     seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="APH (E-value: 4.3e-06, bitscore: 25.7,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGRTLPPRIGPYLVERRLGAGGMGEVYLAYSVAGEPVAVKVIRP
                     DRTDPHTRARFEREATIARTISGTGRVARFIDADPFAEEPWLAMDYVPGRPLSDLVKDQ
                     GPLAAPLVASLGALLAEGLAAVHAVGLVHRDLKAQNVILGDFGPVIIDFGLGAFLGASK
                     GSLSQAGTVIGTVRCMPPEQALGEVDMTQAADVYGLGTVLLYAATEHYPYDGARWEAVA
                     AQVVNPEVDPDLSGLPKELASVVTPMLAHKGDDRPSLEEVTQQCADLMRLLGTSPARAR
                     RALIEETTAKDHSTMVLPQPDAPMLDRLDSLERLLREELVSGERNIPVPSVAGKPGTDT
                     AGQINVEISLSSESLTTLGSEPESGRPAEPVPQRGRPPASRRVADELRERYARRPALAW
                     SKR"
     misc_feature    complement(8028..8030)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8031..8033)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8690..8703)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.92"
                     /tool="antismash"
     gene            complement(9436..10029)
                     /locus_tag="HEP86_RS33960"
                     /old_locus_tag="HEP86_33960"
     CDS             complement(9436..10029)
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571595.1"
                     /locus_tag="HEP86_RS33960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33960"
                     /product="GNAT family protein"
                     /protein_id="WP_248001425.1"
                     /transl_table=11
                     /translation="MEPAISTSSDPTLWIGTDTCALGPYRADLVETYWRWEQDPTLLVG
                     YGRQAPESLEARTEGMAHQLRGGNIRFTIYDRTSGEPVPAGVATLLPDHAVRTAEYIIM
                     LAPEARGRGLGTQATRLALDYAFHITNLRMVWLKVLAPNAAGIRAYEKAGFHAAGNLRE
                     AGYWLGKACDEVIMDAVAGDFDGPSVVKSLLAGK"
     gene            complement(10001..10729)
                     /locus_tag="HEP86_RS33965"
                     /old_locus_tag="HEP86_33965"
     CDS             complement(10001..10729)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520044.1"
                     /locus_tag="HEP86_RS33965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33965"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_168482477.1"
                     /sec_met_domain="PF13471 (E-value: 3.5e-19, bitscore: 67.8,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIELPRFATAPAHVRALDFGHVTVLVNYRNGHVQCLLSAAAALWR
                     DAAATGRLDVMPSVLATRLLTTGLLVRTLTPIPWPAPLIAKGSSASWGSAEHPAGALRP
                     APTRTTTAAVAALATVLAIKRLGTPSTTMPRLTTALRRAVSTCRQPATHDQAQAAVLAV
                     RRAGWYSPARTACLEESAAAVLLLATRRLSATWCHGVAPDPVRLHAWVQTDNGGPAAEP
                     PSTLAYTAALTIGARHQHQL"
     gene            complement(10722..12542)
                     /locus_tag="HEP86_RS33970"
                     /old_locus_tag="HEP86_33970"
     CDS             complement(10722..12542)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457610.1"
                     /locus_tag="HEP86_RS33970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33970"
                     /product="albusnodin/ikarugamycin family macrolactam
                     cyclase"
                     /protein_id="WP_248001426.1"
                     /sec_met_domain="Asn_synthase (E-value: 2e-32, bitscore:
                     112.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFGGFSNPHNPHPRPAGAKPVAARTTTWRLGDGAARSASNDSGR
                     QRILILGLCGATSFEMQCLAGSKLPGDIGWRWPGSYAVVEEAEDGVALHTDPAAARPVY
                     LTAYRGGWAWCTSARALASLIGAPVDTQRLACSVFLPSAPALAGSRTFFTGVEQLPPGS
                     RIELPADGSSPRCTTLWRPDADSAGSPVQRLRSALTATVALRVATDPALSCDLSGGLDS
                     TSVAVLAAHALPPSGRLNAVTIHPESDTSGADLHYARLTAEAHDGRIAHHLLPMAPEHL
                     PYTRITAVPATDEPAPSTLTQARLTGQLRWMRTNLGSHTHLTGDGGDSVLFQPPLHLAD
                     LIRHRRWKRAANEAFGWARLRHHKVLPFLVDATRAARASRRQALANLARTVGTPGRNDH
                     GRVRWFPLLPFPAWAEPAARRLLAQAAREAVAAPDTLPGLDNSVRVLVDEIREVARTAA
                     ADAELAASCGIDLHNPFLDATVMDTVMRTPIDQRAPLHAYKPQLSQAMAGLLPPAVAAR
                     TTKGSFDADHYAGLRANLTGLLALTDGHLAGLRLISPASLGRALRGAAAGIPMPLATLE
                     QAMAAEAWLLAHHRDPAPVWTSEPARSTHD"
     misc_feature    complement(11931..11933)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(12105..12107)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(12613..12744)
                     /locus_tag="HEP86_RS33975"
                     /old_locus_tag="HEP86_33975"
     CDS             complement(12613..12744)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457608.1"
                     /locus_tag="HEP86_RS33975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33975"
                     /product="albusnodin family lasso peptide"
                     /protein_id="WP_168482479.1"
                     /transl_table=11
                     /translation="MPDQLNAPPIEENDLVPIEIGDAAELTEGQGGGNSEDKRRAYN"
     gene            13571..14848
                     /locus_tag="HEP86_RS33980"
                     /old_locus_tag="HEP86_33980"
     CDS             13571..14848
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019764472.1"
                     /locus_tag="HEP86_RS33980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33980"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_248001427.1"
                     /transl_table=11
                     /translation="MGMMAPDGIGAMLRGIRESAGLTRDEQALALQEAQSGKWFDPENL
                     KRWETERRLPTPVWHELLAEVYGRSVEEIVRAVAASRRYRRSQRRAGVEGEEDAAVKRR
                     QFLGVAAGATGAVALPGIAEARQGINAGLSNADTGDLDYLQGAFERHRGGYRGRPPGAV
                     LAQMRNDLDLLRDVLSRPHRASARADLARTAAGITGLVAIIQHDRGDQRDSLGWFATAE
                     QAARESGDRHMLAWSLARHAMVPLNYGAPNAAAELAIRARAEAGRTPTAAAALAAAVTA
                     RALASTGDHKGALRAVADARSIAERLDAAQGADTWFGYPLQKHHVHLSQAFTLMGRTRE
                     AHAEQETALFLTRSPSVMTRALLAMDAATCLKVDGDDSAAADAAADVWQRLPSAYRDGL
                     IRSRADALHQSLVGSAHSRLGDALAS"
     misc_feature    13661..13663
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            <14860..15012
                     /locus_tag="HEP86_RS41120"
                     /old_locus_tag="HEP86_33985"
                     /pseudo=""
     CDS             <14860..15012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482620.1"
                     /locus_tag="HEP86_RS41120"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="HEP86_33985"
                     /product="transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="FQALRITESCAA"
     gene            15009..15563
                     /locus_tag="HEP86_RS33990"
                     /old_locus_tag="HEP86_33990"
     CDS             15009..15563
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766317.1"
                     /locus_tag="HEP86_RS33990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33990"
                     /product="flavoprotein"
                     /protein_id="WP_168482480.1"
                     /sec_met_domain="Flavoprotein (E-value: 1.4e-09, bitscore:
                     36.7, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQPDQPKKPFLYVVVCASGITSDVHKLITAAQKQGWDVGVIAT
                     PLGLGFIDASAVEAQTGYPIRSAWRSPGDPRPLPPADAIAVAPATFNTINKWAAGISDT
                     LAVGILCEAYGLGIPTAVLPYLNTAQAAHPASRQSLDRLREMGVLIGSYDPHRPKAGGG
                     ADRFRWEEALELLAPEVSGRA"
     gene            complement(15565..17277)
                     /locus_tag="HEP86_RS33995"
                     /old_locus_tag="HEP86_33995"
     CDS             complement(15565..17277)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960876.1"
                     /locus_tag="HEP86_RS33995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_33995"
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_168482481.1"
                     /transl_table=11
                     /translation="MVPDVSRGSRTIGLLHYLYRTSDIEAHVDPHLVAAYDPHLPDPGR
                     DPNATFSELAADLDLWVNALGGKAPKKHVWHCPVRTAPGDRVLSDEEWATVAHRVLAAT
                     GIAPDGDDQACRWIAVRHAPDHIHIVATLVRADRTRPRHHKDGTRAQTECRKIEKELGL
                     RRLKEGDGTAAKRPTTAERRKAERLGQDATSRELLREHVHRALSGASDEAEFFDRLTAE
                     GIRIKKRIAPSGDVLGYSVAKVGDRNKAGEPIWFSGSKLAPDLSLPRIRERFAAATDMP
                     EPPVVLERSGASAPVRARHFAAEATDSALTSFTAGNDGAAAAQLIGVGEVLDALAQTSF
                     GPTQAELREAAVHFERATRSHIRAEKEEMYALRRAAHQIVHSGSNRGRGQDGAATALVL
                     DIMILTVIAAARWHAARGHAQQAEAAQQAAAHLRAAYQATAASPLATMRAYGQRLPAQS
                     RRHHAATARTALPHLAERLQAESGWDALTAVLDQAERTGHDTTALLAKAAAQRELDSAA
                     SISEVLVWRLQHLGYITQPASAPRSRRPRTEPAPAVPAGSGVDHQEANRLRRR"
     gene            complement(17283..17852)
                     /gene="mobC"
                     /locus_tag="HEP86_RS34000"
                     /old_locus_tag="HEP86_34000"
     CDS             complement(17283..17852)
                     /codon_start=1
                     /gene="mobC"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /locus_tag="HEP86_RS34000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_34000"
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_168482482.1"
                     /transl_table=11
                     /translation="MHNTNHEATSQYDSQEDRNSTTSGASCSTGPSKGRSRAHASAPEV
                     AEEAPRQGAQDRQLVAEGGHKQRVKQGRRRSRQPRQRSERLTFRASPTESEDIQHAADA
                     KGVSKARFIAQAVHSELHGRPHLDQDDALDRLEAARVQLVRAGNNLNQIAKVLNSGGDA
                     LHIDRAADAVAEAATEIRTAAQKLVS"
     gene            complement(18781..19629)
                     /locus_tag="HEP86_RS34005"
                     /old_locus_tag="HEP86_34005"
     CDS             complement(18781..19629)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483582.1"
                     /locus_tag="HEP86_RS34005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_34005"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_168482483.1"
                     /transl_table=11
                     /translation="MSHAAREWVWDHSTSKGTARMVLTLIADRCVDRGCVAYASLPTLM
                     KRANASRTAVRDALAKLIATGELEQLMGRKGPRGETYYRLPAAARFLADQAIDEGRNPT
                     LQGERIPTPEGTESDPAAPFEGGPESDPGERNPTPRGHGTQPSRSTDSDPQNSREPKVN
                     GKSSSSAPLIPATQWQIDEDTRAWLQHQGHLARLGDHALRTADEKWRSYRAVGIPRTAA
                     AWAADWRAWIARERTPTPGHPHLRALPGGAPTSTSAGGMTRAEAHTAALLAALDEPAET
                     E"
     misc_feature    19877..19892
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.84"
                     /tool="antismash"
     gene            20179..20474
                     /locus_tag="HEP86_RS41990"
                     /pseudo=""
     CDS             20179..20474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009329297.1"
                     /locus_tag="HEP86_RS41990"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="XRE family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAVRPLEIGLAGTRTTRNIEILRTERGPA"
     gene            20524..21426
                     /locus_tag="HEP86_RS34015"
                     /old_locus_tag="HEP86_34015"
     CDS             20524..21426
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262610.1"
                     /locus_tag="HEP86_RS34015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_34015"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_211117488.1"
                     /transl_table=11
                     /translation="MAAIVIVHGVGNQYLGKHTLHSSVAPALLDGVRLADGPPLKPDDV
                     EVAFYGHWFRPPGTTSVKGEPAYAHRDVMGPFETELLLALWREANRLEPDRVPAPDLTC
                     TADTGTARSKAAVPYSVQRALYALSRSSFLAGVADRFLIGVLKQVHRYLTDDLVRDSVQ
                     REIADCVTPETRVLVGHSLGSVIAYEALCAHPEWPVTALVTIGSPLGIPNLVFDRLRPA
                     PRAGRGAWPGGVRRWNNLCDRRDVVALAKRLAPLFPDPTRTVDDVLVDNGWRAHALEHH
                     LTAAETGAAIAEGLAGGAR"
ORIGIN
        1 caggttcggg gaagatccgc ctctgattga caaacccgcc gtgtacccgc aaccgctgga
       61 acgccgcagc cctcagagcg gcctctttgt cacccgcgaa atgggtgtcc ggatgcacca
      121 tcccgcccgt cccgccgcgc ccagcgtggt cccaaatctc gcacatgaaa gcccggtaca
      181 gatcgggccg agtgcctgcc acgagtgggt acaccggtgt cgccgccagg tacgacgcca
      241 tcgttaccgt attcgtcacc tcgccaagca gatactcctg cacctccccg cgcgcgagtt
      301 cgacgtcccg ccgccggttc ttctcctccg ccttcggctt ctcagccagc atgaaccacg
      361 gctcgtactc cgcgaggacc ggatcctcct tccactccgg ccgcacccac ggtggattgc
      421 ccacctgaag gtcgaagccc cctgcctccc cggcaaacac gccagcgaag tccaactccc
      481 agtgcaggaa tccctgttca tcggcgatat cgcgaaccac ccgcatccac ggataccgcg
      541 tctccaccgc cttacgggca tcgtccatgc ccatctcggc ctggaccaga tcttcaagag
      601 ccttcagctc ttccagggag tcgaccgtcg tggcaaaggt gtcctccggg atcgacgccg
      661 tgcccaccat ggattccagg aaggcgatcc agtcctccat gtccacgagc ggaatgacgt
      721 cccgccgacg cagcggcccg ccgggcttct tgggggcgga gggcgtacgc gccttcttga
      781 cctccctctt ctccacgagc tcgccgtcgt ccctggcctc gcccaaggac atcgggtcac
      841 cgtccagcgc gaacagcagc ccgttctcca cgaattgagg cgcgggcgtg ggctcgggca
      901 caggcggctg ctcgacctca acaggctgcc cggccaccga cgacagcaac gcatccaggt
      961 caacacccac ctgtgccgtc accaccggat ccgagtcgta ctccgcgtcc gacccgtcca
     1021 gcagtcccac cttctccagc ggccagaacc acagtgcaca ccatgcgtcc atgacctgct
     1081 tgaggcgcca gtaaggggag ccctcggccg cgaagaggtc tgtgaagacc tgctccttgc
     1141 tcagtggcag gctctgatcc gcgttctgct tgtcatggcg gaggaaggcg tactcctccg
     1201 cgtcctcctc acgatcggcg ccccacacat ggatggtgcg ggcgatctcg cgctcgctca
     1261 gctccatgcg cttggcgacg agtttccaca agaactccac ccggcaagcc gcagcctgca
     1321 accgggcgag ctggctgctc ttgccgcccg tcgtcttggg cttcttctgg acaccgttct
     1381 tccatgcctt cagctgctcg acctgcgccc cggcgagctg cgtgaccaac ttcttcgcat
     1441 ccccgctcgc ccccgcgacc gcaccccaac cgggcgtggg cagcaggaat tggtgcacgg
     1501 ccccgtccgg cagcgactgc tccgcatcgc cctccggcgc ccggaacggc agcttggtcg
     1561 gcgtctgcgc gccgatggtc cgctccttct tgatgcgctc ggcctcgtac acccaccgtc
     1621 gcgccccgat aagggagttg ccgcgccgca gatgcagacc gaaccacggc gcgcgcatgc
     1681 cggggtgcat ggtgttgagc cacagcgaca cctccgccag ctccacaccc gtcgagttca
     1741 ggtcgatccc gtacgcgttg tggagggcga tgtacgcctt caccttctgt tccgcttcga
     1801 gcgccttcga cgtgtccagg tggacgccca gttcatcctg gcggcgcttc agatactcct
     1861 tcgcgacctg gttgatcgcc tcgctgagga acgcgccgga gcccagggcc ggttcgcaga
     1921 tcgtgtagcg cagaagctcg ctcgcgcgag tccgaaccgt gttgccgtcc gcgtccttct
     1981 cctggtcgag gcggtgcttg agggccagtt cgacggtgac ctcggtgagg gactcgggag
     2041 tgtagtacga ggctgatgtc tcgcggtcgc ggccggccag acggtagacg aacctgccct
     2101 tggcgtattt cttggagccg cgcagaccgt actgttcggt gtcctgggtg gagtacacaa
     2161 cgtgcgtgtt gtccgggtac tccttcagcc gctcggcggg gatgagccag gatcccttct
     2221 ccgggtcgcc gtactgcttg tcgcccttct tcgcgccggg cttggcgacc tctgccagct
     2281 cgtccttggc gatgatgccg gtgtacgaca tcagcccctc gtacacggca ccgagctggt
     2341 tgatgcccag gttgcggtag gagatgaagc ctccctgtct gcccttctgg ccggcctgct
     2401 tcatggtcag caggcgcagc acctggtgca acgcctcgtt gcgcagccgc aggtccagcc
     2461 agcgcggcgg ctcgtccccg tcctcgtccc accgggggtc gaggacacgg cggccgatga
     2521 ggtcgatcgc cttcgggtcg aacagctcgc tgcgcagcgg ctcgaagcgc aggccgcgca
     2581 gttcactacg cttcttggcg ggcttgtcgt cgtccgcctc ggtgccatga ggtcggtggc
     2641 cgtagttgac cttgttgaag aggacgtcga gggaggcgta caggtggaag ccgcctcggc
     2701 tttcctcgtc gaccagcttc ctctcgcgag cgaccagttc acgcagccgg gcgatcgagt
     2761 acccagcctc gtacgtctcg tcgttggcag gaaggatgcc cagctcgggg cgagcctccg
     2821 cgtacaagag gaagaggatg cggtagaggt agcgcaggga ttcgcgggtg agctgcttgg
     2881 cgaacgggcc cttcttcagg tcctcgatct cccgtggctc gacctccgct ttgcgtaacc
     2941 gggcgagcac ctcgtttgcg atgatctcga cggagtgctg gatccccttg cgcagctcgg
     3001 agttgacgcc gacggcgttg tcacgggacg ccttcaggag gtcgtcgatg cggcggccct
     3061 tgccgtcgag ccggggcgcg agcatgtcgt gggagaagag ggcggcgagg agcgcgagtt
     3121 cgccggcctt cgcggtgtcg ttgcgggtga gggccgcgtc cagactggcg gcgagatagc
     3181 ggccttcggc ccaggcgcct cggtcggcga ggacgagtac gccgccgcag agcagcagga
     3241 cgaagcgggg tgcgttgcca ccgggctcgt gcagttcgct ctggaagagc cagtccgcca
     3301 gcttctcgcc gacttcgagg aggccgtcag cggatttgtg ggggtggagg agccgtccgg
     3361 ccttgtcggg gtcgagggcg tcgtcgagtt tggcggtcca gccgcagtcg agggcgacga
     3421 tgccgtcgcc gtgccaggcg acctggagtg cgtactccct gcccgcgttg tgcacggtga
     3481 gcggctcggg ccggtcaccg ccgtagccga gggcctcgag gacggtggcg tgccagatgg
     3541 tgaggcgttc gggccactca cccgtcaggt cgtcgtaggt gctggcgagt tcctcctgct
     3601 gggcgcggga ggccaggaag gggcgcacgg tggacaggta ttcaccgcgg agccggggca
     3661 ggagctcgcg cggggtggcc tgcgggtcgt gcgggtcggt ctcacgcccg gtccacaggg
     3721 tgaacagacc ctcgtcgcct gccttgctct tcttgagggt gttctccagc tcctcgctga
     3781 agtagtgggc ggagaggtac tcgccgcggt tggcgaagga gtcgtaggtg tagctcatcg
     3841 tgcggactcc tcggcgtgcg cggcggacac ggtggtggtg tgcaggggtt cgaggacgca
     3901 gaggacccgc agcatcgggt cgccgtcggt ccgcaggcgg cgtacgagct tgcggcggcg
     3961 ggcggcggtg tcgtacacct gcttgcggcg ttgctgctga ttgcccacgg cgatcagggc
     4021 gccctgctcc cagacctgta cccggtcctc gtacggggcc aggtaggagt cgacttgctt
     4081 gtcgtagtcg acacggcgct cgcgcaggtg ggtctcggcc gcgtcgacgg ccgcggggac
     4141 gagtgcctgg aggaggtcgg ggtcgacggg ccggccgcgg ccgggcatgc cggggcccac
     4201 accgcaggcg gcgaggaacg ccgagtccat gcgccatacg cgaggagcgg cctcggcgag
     4261 accggtgacg gccatccact ccacgaccgt cggtttcccc gccgcgttgg agtagaggcc
     4321 ttggaggagg tagacggggc tggtcagggc ctcgggcagg gcggcatcga tccgtttggc
     4381 cttgtcggcg tcgggttggt gggcgaggac gaatgcctcg tcgtaccgga gcttggcgag
     4441 gaccttgtcc gtcacccagt cgagcacggg atggatgtcg gagacgtagg agacgttggg
     4501 ccactgggaa gtgggctggt cggtcttgcc ctgcttggcg ttgcgggcgg cgtccaggcg
     4561 gtcggcggcg taccgcttgt cgaaggtgag gcgcagtgtg ccgtcgtact tgggagtggt
     4621 gaggatcttc tcctgctcca ggtacgactt cggaagctcc cggaagcggt actgcaggtc
     4681 gccgggcgga gtgaactcga tgcgcccgtg ggccgtttcg ggcttccagt cgagagcctt
     4741 gcgctcggcc tgcgggtaga tgaggtccat cgcggcgtgg aagtacgcct tggtgtcggc
     4801 gaacacgtgg ggcacgtcgg cctcgggcac cgagcccgtg ccgagggtgg ccggagcggc
     4861 tgtcgggtcg gcgagccggg cgttcgcgcc ggcgaggagg tcggcgagga ccccgccgga
     4921 ctcctgctgg gactgcttga tggagcgttc gacggtgccg cccttgatga ggtcgcgggt
     4981 gaggcggtcc tcttccttct tgtcgtcgta gaggccggtg acggcctcgg cgctgccctc
     5041 gccggtctcg atctcgtgcg cctgggcctc gcggcgcagc aggcgggcgc cgaccagccg
     5101 gtcgtcgagc gtgagcgtcc tgccgctctc ctcgtcgcgc cgccagggca cgtcggcggt
     5161 gagggtgatc gcacggaact cggggctcac cgcctgcccg tagcggtcga tgcgcccgtt
     5221 gcgctgctcg atgcggatga gagaccaggg gaggtcgtag tgaacgaggt cgtggcattg
     5281 gtggtgcaga ttgacgccct cggatgcgat gtcgccggtg aagagcagcc gcaccggctc
     5341 gtcggcccga ccgaagcggt cgatgatctc tcgctgctgc tggtcgttgg tggtgtcgcc
     5401 gtgcatgacc tcgacgacac cgccgtacgc ctgccagggg cgcgtcttgt cggccttggc
     5461 cgcgggtttc ttcttgaagc cgagcagggc cgggacctcc tgggccagcc agtccagggt
     5521 gtggacccgc tcggagaaga tcacgacgcg gcgctcgcag cccgggccga cctggaggtc
     5581 gtccttcaat gtgcggacga gcgcgtcgag cttggcggag tcctggtcgg tgaaatccgc
     5641 gaccagtgcc tccagttccg cgagcgctgc accctctgcc gccagggcgg cgcggcgctc
     5701 gggagagtcc gccttcttgc cgtccttgcc tttcaccatg gcgctcctgg ggttgtccag
     5761 gtaggcgcgg cgggcggtga gggttttgcg cagggcgacg tgggaggaga ggaacgcctt
     5821 caggaagttg tagccgacca gcggatcggc acacaccgac gaacggttcg gctccccggg
     5881 cgtccactcg ctcgcgagtt tctccaggac ggcgacctcc ttgcgcgtcg ccggagccgg
     5941 caccggcagg gactcgcccc gttcggccca gggcttgtcc ttgaggccgt cgcgcacctc
     6001 gcggtcggac ttggtgcgcc ggatgtagag gtggtcgagg gcggccacct tgtagttctt
     6061 ggggtcggcg atcgccgccg gatcgagcat cccgatcagc tcggcgaagg actccgcgtc
     6121 gccgttgtgc ggagtggcgg aggccaggat cagtgcgtcg gtctgttcgg cgaggcggcg
     6181 ggcgaggcgg ttgttgcggg tgcccttgtt cacgaggttg tgcgactcgt cgatgaccac
     6241 cgcgtcccag gtgatcttct ccaggtggtg ggcgtacgtc gccgacttga gggtgtcgac
     6301 ggagacgatg acccgcttga agtgggcgaa cgggttccgg cccgccggga tctcctgctg
     6361 gatgcgctgg ataccggtgg agtccaggcg caccagcggc agggcgaagc gggtccacaa
     6421 ctcgcgctgg aactgctcca acacatgggc gggtgtgacc acgagaatgc gctcgccgcg
     6481 cccgcgccgg atcagctccg agagcgtgac gccgatctcc aacgtcttac ccagaccgac
     6541 gacgtcggct atcagcagcc ggggctgtgg gttgcgcatg gacagcgcca gctcggcagg
     6601 gcgcagctga tgctcctgcc ggtccatgag gaaggagccg gcgagcgcga tgccgtgctc
     6661 ggtctgcggc agcgcggtct tgcggatgac cgcctccagg tagaggcggg ccttggcgtg
     6721 tttggaggtg ccgtcaggca cgagccgggt cttgcgcgga tcgagtacgt cgatctggtc
     6781 gaggcccgag tagaacactg cgtccgtgcc acggacgaac ggggaaaccc cgctgacctc
     6841 gaccatcagt ccgtcgcggg actccccgag ggacttcacg agccactgct cgtcgcggat
     6901 gcgcacctgg gcgccagggg ctaagaccgg cttcgcgccg gtcgggtacg gcggatgtgc
     6961 cggatcggcg tcgcccggct cagggacctc tggctgcatc gccgtggtca cgtggtgctc
     7021 ctctgtgaac ggtctactcg tgctgtccgg tgtgctggtg gggaagcctg cactgtcgtc
     7081 cggtgagctg ggtgcgtttt ctccagggcc atcccgacgc cccgagttcc gtgctgtgtc
     7141 ggctcgttgg cgggagaccg gaggagggca gcggctcagc gcttcgacca ggcgagtgcc
     7201 ggtcgtctcg cgtagcgctc gcgcagctcg tcggcaaccc tgcgggaagc ggggggacgg
     7261 cctcgctgcg gtacgggctc cgccggtctt ccgctctcgg gttcggaacc gagcgtggtc
     7321 aaggactcgg acgatagcga gatctcgacg ttgatctggc ccgcagtgtc cgtaccgggc
     7381 ttaccagcga cgctggggac agggatgttt cgctccccgg acaccaactc ttcgcgcagc
     7441 agtctctcga gcgagtcgag ccggtcaagc atcggcgcgt ccggctgcgg aagcaccatc
     7501 gtcgagtgat ccttcgccgt cgtctcttcg atcagagcgc gtcgcgcgcg ggctggactc
     7561 gtacccagca gacgcatgag atcggcgcac tgctgggtca cctcctccaa agaggggcgg
     7621 tcatcccctt tgtgcgccag catcggcgtc acgacggagg caagttcctt gggcagcccg
     7681 gacagatcgg ggtcgacctc ggggttgacc acctgcgcgg ccaccgcctc ccaccgggcg
     7741 ccgtcgtacg ggtagtgctc ggtcgctgcg tagagaagca ccgtgcccag gccatacaca
     7801 tcggcggcct gcgtcatgtc cacctcaccc aaggcttgct cgggtggcat gcagcgcacc
     7861 gtgccgatga cagtgccggc ctgggagagc gagcccttgg aggctccgag gaaggcaccg
     7921 agcccaaagt cgatgatcac tgggccgaag tcgcccagaa tgacgttctg cgccttcagg
     7981 tcgcggtgga ccaggcccac ggcgtgcacg gcagcaaggc cctcggctaa taatgcgccc
     8041 aggctcgcca cgagtggcgc ggcgagaggt ccctggtcct tcacgaggtc ggagagaggg
     8101 cggcccggga cgtaatccat ggccagccat ggctcttcgg caaacgggtc cgcgtcgatg
     8161 aaacgggcta cacggcccgt gccggagatc gtgcgcgcga tcgtcgcttc gcgttcgaag
     8221 cgggcgcggg tgtgcggatc cgtgcggtcg ggccggatca ccttcactgc gaccggttca
     8281 cccgcgacgg agtaggcgag gtacacctcc cccataccgc ccgcgccgag ccgccgctcg
     8341 acgaggtacg gaccgatacg cggcggcaaa gttctgccgt tcaccgcacc cccctgactt
     8401 ccgtaacgac agcgcgacct cgctcggtgc gtgcatccac gccgccgcac aagaacggcg
     8461 tgggtgacgt ggacaccgac gaatcactgc tacaagcgta ctgatcgaca tcccgccgta
     8521 ggaagcgatc acacagaccg gatcaccttc cggcctctac gtccgggacg ggccgctgcg
     8581 gaggggttcc ctgatctccg ggtacggcat tccatccggt tcagatgccg ctgcatggcc
     8641 ctcagagcgc ggcggaaggc gatgtcttgg tcgtcaaccg ggtcgggact tgttctgcat
     8701 cagagcattt cggaggcctg cacacagcgg atccctgaac ggcagcgtgc tcggcgagcg
     8761 cggagccttt gagcagcgca cggcacgcga gtaatgctgt agcgctgggg tcggcggccc
     8821 aggagtgacc gcgagaatcc cgaaacctgt gcagcatatg aagacgcttg atgcgccgac
     8881 gtgcggctcg cgcggttgcg cggggcggcg ggccccgcgc gggagaccgc gggactgcgc
     8941 ggtgcgcgag gcgctggtgg tggcgcgctg ccagcgggtg cgaaggggga cgatgccggc
     9001 cagggcgcgg cgacttccgg tgattagctg gtgcggcgtg ttggccggat cctcggcgta
     9061 ggcactgatc cgagccgcca cttcgaacgc ccgccttaac agggcgtggt gaagtatgtg
     9121 tttgccccag cactcagggg atcctgtcgg tgtagagaca agggccagca ggcaggcggc
     9181 agtgatgtcg tgagtgaggg ttccgcggtg ttgggcttcc cggagtacgg tgacgggtcc
     9241 acggactctc cgcggtgcac cagcgaggtg aggcactgcg agagcgtggt gtgcagaaga
     9301 gaaggccctg gctcgggtgc attggctgaa gatccgatct gaacgaaccg ccaacagccc
     9361 agagaaccat cgagcctcgt gactggacgg aaaaccctgt cgggtcggcg agcactttca
     9421 tgctggcgta gcgccttact tgccagccag tagggattta acaaccgatg ggccgtcgaa
     9481 atcccccgcg acggcatcca tgatgacttc gtcgcacgcc ttcccgagcc agtacccggc
     9541 ttcacggagg ttgccagcgg cgtggaagcc cgccttctcg taggcccgga tgccggctgc
     9601 gttcggggcg aggaccttga gccagaccat gcgcaagttg gtgatgtgga aggcgtagtc
     9661 gagggccagg cgggtggcct gagtgccgag accgcgcccg cgggcttcgg gggcgagcat
     9721 gatgatgtat tcggcggtgc ggacggcgtg gtcgggcagg agggtggcga ctccggcggg
     9781 tacgggttcg ccggaggtgc ggtcgtagat ggtgaagcgg atgttcccgc cccggagttg
     9841 gtgggccatg ccttcggtgc gtgcttcgag ggactcgggt gcctggcggc cgtagccgac
     9901 gaggagagtg gggtcctgtt cccagcgcca gtaggtctcg acgaggtcgg cgcggtaagg
     9961 gccgagtgcg caggtgtcgg ttccgatcca cagggttggg tcagagctgg tgctgatggc
    10021 gggctccaat ggtgagggcg gcggtgtagg cgagcgtcga gggcggctcg gcggccgggc
    10081 ctccgttgtc agtctgcacc caggcatgga gccggacggg atctggcgcg acgccgtggc
    10141 accaggtggc cgacagacgc cgggtcgcga gcaggagcac cgcggcggcg gattcttcca
    10201 ggcaggcggt tctcgccggc gagtaccagc cagcgcggcg gacggcgagg acggcggcct
    10261 gggcctggtc gtgggtggcg ggttggcggc atgttgacac cgccctcctc agcgcagtgg
    10321 tgaggcgggg catcgtggtg ctcggggtgc cgagtcgctt gatggcaagg accgtggcca
    10381 gagcggctac ggcggccgtg gtggtgcggg tgggtgcggg ccggagggct ccggcagggt
    10441 gttctgcgct gccccagctc gcgctggagc ccttcgcgat cagcggcgcc ggccacggga
    10501 tcggtgtgag ggttctgacc aggaggccgg tggtgagaag ccgggtggcg agaacggacg
    10561 gcatgacgtc caggcggccg gttgctgctg cgtcgcgcca gagcgccgcc gcggcggaca
    10621 gcaggcattg gacatggcca ttgcggtagt tgacgaggac cgtgacgtgc ccgaagtcga
    10681 gggctcggac atgggcgggg gcggtggcga agcggggcag ctcaatcatg tgtgctcctg
    10741 gcgggctcgc tcgtccagac aggtgcgggg tcgcggtggt gggcgagcag ccaggcttcg
    10801 gcagccatgg cttgttcgag ggtggccagt ggcatcggga tgcctgcggc ggccccgcgc
    10861 agggcgcgtc ccagggaagc cgggctgatc aatcggaggc cggcgaggtg gccgtcggtg
    10921 agggcgagga ggccagtcag gttggcgcgg aggccggcgt agtgatcggc atcgaagctg
    10981 cccttggtgg tgcgggctgc tacggctggc gggagcagtc ctgccatggc ttggctcagc
    11041 tgcggcttgt aggcgtggag cggtgcgcgc tggtcgatgg gtgttctcat cacggtgtcc
    11101 attaccgtgg cgtcaaggaa cgggttatgc aggtcgatac cgcaggaggc tgccagctcg
    11161 gcgtcggccg ctgccgtgcg tgcgacctcg cggatctcgt ccaccaagac tcgtacggag
    11221 ttgtccaggc ccggcagcgt gtctggtgca gcgacggctt cgcgcgctgc ctgggccagg
    11281 aggcggcggg ctgccggctc ggcccaggcg gggaacggca gaagagggaa ccagcggacg
    11341 cgcccgtgat cgtttcggcc aggcgttccg acggtgcggg cgaggttggc gagagcttgg
    11401 cgacggctgg ccctggcggc cctcgtggcg tcgacgagga acgggagtac cttgtggtgc
    11461 cgcaggcggg cccagccgaa cgcctcgttg gctgctcgct tccagcgccg gtgacgtatc
    11521 aggtcggcga ggtgaagggg cggctggaac aggacgctgt cgccaccgtc gcccgtcagg
    11581 tgcgtgtgac tcccgaggtt cgttcgcatc cagcggagct ggccggtcag acgggcctgc
    11641 gtcagggtgg agggggctgg ttcatcggtg gccggcacgg cggtgatgcg ggtgtacggc
    11701 aggtgttccg gtgccatcgg cagcaggtgg tgggcgatgc ggccgtcatg cgcctcggcc
    11761 gtcaggcgcg cgtagtgcag atccgcaccg gacgtgtcgc tttcggggtg gatggtgacg
    11821 gcgttgagac ggccggaggg cggcaacgcg tgcgctgcca gcactgcgac ggaagtcgag
    11881 tccagtcctc cggacagatc gcacgacagg gcaggatcag ttgccacgcg taatgcgacg
    11941 gtggccgtca gcgcactgcg cagacgctgc acgggcgatc ctgccgagtc ggcgtcgggc
    12001 cgccacaggg tcgtacacct ggggctgctg ccgtcggcgg gcagctcgat tcggctgccg
    12061 ggcggcagtt gctcaacgcc cgtgaagaac gtccgtgagc ccgctaacgc gggggccgag
    12121 ggcagaaata cggagcacgc cagccgctgg gtgtctacag gggcaccgat gagcgatgcc
    12181 agtgcccgag cagaggtgca ccacgcccac ccgcctcggt aggcggtgag gtagacgggg
    12241 cgggctgcgg ccggatcggt atgcagggcc acaccgtctt cggcttcttc cacgaccgcg
    12301 taggagccgg gccatcgcca cccgatatcg ccgggcagct tgctgcctgc caggcactgc
    12361 atttcgaagc tggtggcacc gcagagcccc aggatgagga tgcgctgccg accgctgtcg
    12421 ttcgatgctg agcgagcagc gccgtccccc agccgccatg ttgttgtccg tgccgcgacg
    12481 ggcttcgctc cggcgggtcg ggggtgggga ttgtgcggat tgctgaagcc tccgaagatc
    12541 actaattcct ccaagggggt tcggacgcag gtgcggtgcg gccccaggct ggggccgcac
    12601 cgcacttgtt gatcagttgt aggcgcgacg cttgtcctcg ctattgccgc cgccctgtcc
    12661 ctcggtcagc tcggccgcgt ctccaatctc gatgggcacc agatcgttct cttcgatcgg
    12721 gggcgcattc agctggtcgg gcatggagac tctccttctt tgtgtggaat atgcggtggg
    12781 tgttgctgcc aggccctctc gacggggtgg agtgtcgaag gggactgggg gctggggtgc
    12841 cgtccatggc gtgcgcgatg tcctgccggg cccggtctcg ggcgccggcg gggcgcggtc
    12901 aggcggcggg cgacggctcg ggcggcgcga ccggggcgaa gaatgagctg gcggcgcgtg
    12961 agccgctgtt cggggagggc gatctctgcc catacgcact tgccgcactt ggttcgttcg
    13021 gctccgtggc agacggacag acggtcgatg agcgccatac cgcgtccggt ctcgtcctcc
    13081 ggagccgcgc ggcgcgggaa ggatgcgcga gccgtcgtag acctcgacca ggacccgtcg
    13141 ccggctgagg ttggcgcgca ctccgatggt gagcatcgcg ccggtaccgt gccgcaccgc
    13201 gttggtgacc agttcggaga gcaccgtggt cagcgctgtg tcggaggctt cgttcaggcg
    13261 cagcccccag ttctcggcca aatcgtggag gcggcgacgg ctgagccgca ccgaggtcgg
    13321 gtcgcggacc agaaagaaac tttggctgta cacgcgcatg gcgaggacct tccaacggac
    13381 atcggaccgg ggattgaggc ggaggagccc ggaaggcggg ggattgaacg tcttccgcct
    13441 gcctgcttgg ctgaggcaca gtcaactcgc ctgcggcaga ccggtccggt gccagcgagg
    13501 ggtgcgattt cgcgcagggg gccacattgg ggggccagga atgggctcgg cactgtcaga
    13561 cgcttggggg atgggcatga tggcaccaga cggcatcggc gccatgctga gaggtatccg
    13621 ggaatcggcc gggctcacgc gcgatgagca ggctctggca ttacaagaag cccagagtgg
    13681 aaagtggttc gacccggaga acctgaagcg ctgggagacc gagcgccgtc tgccgacgcc
    13741 ggtatggcac gaactgctgg cagaggtcta cggacgctct gtggaagaga tcgttcgtgc
    13801 ggtggcggca agcaggcgct accgcaggtc gcagcgccga gccggtgtcg agggagagga
    13861 ggacgcggcg gtgaaacgac gacagtttct cggggtggcg gcgggagcga caggagcagt
    13921 cgccctgccg ggtatcgcgg aggcgcgaca gggcatcaac gccggcctct ccaacgctga
    13981 tactggcgat cttgactacc tgcaaggggc gttcgagcgg caccgcgggg gctatcgtgg
    14041 ccgcccgcca ggcgccgtcc tcgcccagat gagaaacgac ctcgacctcc tgcgggacgt
    14101 gctcagccgt ccacaccggg cgtctgctcg tgcagacctc gcacgcacag cggcggggat
    14161 caccggcctg gtggcgatca tccagcacga ccgcggggac cagagggact cgctcggctg
    14221 gttcgccacc gccgaacagg ccgcacgcga gtcaggcgat cggcacatgc ttgcctggag
    14281 cctggcccgg cacgccatgg tcccgctcaa ctacggcgcc ccgaacgcgg ccgctgaact
    14341 cgccatccgc gcccgcgccg aggccggacg gaccccaaca gccgcagccg cgctggcagc
    14401 cgccgtcacc gcgcgagccc tcgcttccac cggagaccac aagggcgcct tgcgcgcggt
    14461 cgccgacgcc cgctccatcg ccgagcgcct ggacgcggcc caaggtgccg acacctggtt
    14521 cggctacccc ctccagaagc accacgtcca tctctcccaa gcgttcacct tgatggggcg
    14581 cacccgggaa gcgcacgccg aacaggaaac ggcgctcttc ctcacccgat cgccttccgt
    14641 catgacccgc gccctgctgg ccatggatgc cgcgacctgc ctgaaggtcg atggggacga
    14701 ttccgcagcg gcagatgcgg ctgctgacgt atggcaacgg ctcccctcgg cgtaccgaga
    14761 cggcctgatt cgctcccgcg ccgacgccct gcaccagtcg ctcgtgggca gcgcccacag
    14821 cagacttggc gacgcgctgg ccagttgaac accgtcgttt tccaggccct gcggatcaca
    14881 gagagctgcg cggcctagga catcagccgg cttgccgtgc gggaactggt tgctgacatg
    14941 gccgcccatg accgccgccg caccctgccg gaactgcacc aagtcagcag ccaaccggga
    15001 gcccgcgcgt gagtgatcag cccgaccagc cgaagaagcc gttcctgtat gtcgttgtgt
    15061 gcgcgtccgg tatcacgagc gacgttcaca agctgatcac cgctgcccag aagcagggct
    15121 gggacgtcgg cgtcatcgcc actccactcg ggctcggctt catcgacgcc tcggcggtcg
    15181 aggctcagac cggctacccc atccgctcgg cctggcgctc acccggcgat ccgcggcccc
    15241 tgccgcccgc tgacgccatt gcggtcgcgc cggccacctt caacaccatc aacaaatggg
    15301 ccgcagggat ctccgacacc ctcgcggtgg gcatcctgtg cgaggcgtac ggcctcggca
    15361 tccccaccgc cgtgctcccg tacctgaaca cggcccaggc cgcccacccc gcgtcccggc
    15421 agagcctgga ccggttgcgc gagatgggcg tcttgatcgg ctcgtacgat cctcaccggc
    15481 cgaaggcggg cggcggagcg gaccgtttcc gctgggagga ggcgttggag ctgctcgcgc
    15541 cggaggtgtc ggggcgagcg tgaatcaccg ccgccggagc cggttcgctt cctggtggtc
    15601 gacgccggaa ccggcaggga cagcaggggc gggctcggta cgaggacgcc gggacctggg
    15661 cgccgacgcg ggttgggtga tgtagccgag gtgctgcaac cgccagacca ggacttcgct
    15721 gatagaggcg gcgctgtcca gttcgcgttg agcggcggcc ttggccagca gggcggtggt
    15781 gtcgtggccg gtgcgttcgg cctggtcgag cacggcggtc agcgcgtccc agccagactc
    15841 ggcttggaga cgctctgcga ggtgagggag ggcggtgcgg gccgtggcgg cgtgatgtcg
    15901 ccgggactgt gcaggcagac gttggccgta ggcgcgcatg gtggccagcg ggctggcggc
    15961 ggttgcctgg taggcggcgc gcaggtgggc tgcggcctgt tgggcggcct cggcttgctg
    16021 ggcgtggccg cgggctgcgt gccagcgggc ggctgcgatg acggtcagga tcatgatgtc
    16081 gaggaccaag gcggtggcgg caccgtcctg tccgcggccg cgattggatc cggaatggac
    16141 gatctggtga gccgcgcggc gcagggcgta catttcctcc ttctcggcgc ggatgtggga
    16201 gcgagtggcg cgctcgaagt gcactgctgc ctctcgcagt tcggcctggg tcgggccgaa
    16261 tgaggtctgg gcgagggcgt caagaacctc accgaccccg atcagctggg cagctgccgc
    16321 accgtcattg ccggccgtga aggacgtcag ggccgagtcg gtggcctcgg cggcgaagtg
    16381 gcgggcgcgg accggtgccg aggcgccgct gcgttccagg accacgggcg gctcgggcat
    16441 gtccgtggcg gctgcgaacc gctcgcggat ccggggcagg gacaggtccg gtgcgagctt
    16501 ggagccggag aaccagatgg gctcgcccgc cttgttgcgg tcgccgacct tggcgaccga
    16561 gtagccgagc acatcgccgg agggggcgat gcgtttcttg atgcggatgc cttcggcggt
    16621 gagccggtcg aagaactcgg cttcgtcgga ggcaccggac agggcacggt ggacgtgttc
    16681 gcgcagcagc tcgcgagagg tggcgtcctg gccaaggcgt tcggccttgc ggcgttcggc
    16741 agtggtaggg cgtttggccg ccgtgccgtc accttccttg aggcggcgaa gtccgagctc
    16801 cttctcgatc tttcggcatt cggtctgggc gcgggtgccg tccttgtggt ggcgggggcg
    16861 ggtgcggtcg gcgcggacga gggtggcgac gatgtggatg tggtcggggg cgtggcggac
    16921 ggcgatccag cggcaggctt ggtcgtcgcc gtcgggggcg atgccggtgg cggcaaggac
    16981 gcggtgggcg acggtggccc attcctcgtc ggacaggact cggtcgccgg gggcggtgcg
    17041 gacggggcag tgccacacgt gcttcttggg ggctttgccg ccgagggcgt tgacccacag
    17101 gtcgaggtcg gctgccagtt cgctgaaggt ggcgttcggg tcgcggccgg ggtcgggcag
    17161 gtgggggtcg taggcggcga cgaggtgcgg gtcgacgtgg gcttcgatgt cgctggttcg
    17221 gtagaggtag tggagcaggc cgatggtgcg gctgccgcgg gagacgtcgg ggaccaaggc
    17281 gttcagctca ccagcttctg tgcggccgta cggatctcgg tggcggcctc ggctacggca
    17341 tcggcggcgc ggtcgatgtg gagggcgtcg ccgccggagt tgaggacctt ggcgatctgg
    17401 ttcaggttgt tgccggcacg caccagctgg acgcgggcag cttccaagcg gtcgagggca
    17461 tcgtcctggt cgaggtgggg acggccgtgg agttcggagt ggacggcctg ggcgatgaag
    17521 cgggccttgg agacgccctt ggcgtcggca gcgtgctgga tgtcctccga ctcggttggg
    17581 ctggcgcgga aggtcagacg ttcggaccgc tgcctgggct ggcgggaccg ccgtcggccc
    17641 tgcttcaccc tctgcttgtg gccgccctcg gccacgagct gccggtcctg cgccccctgg
    17701 cgcggggcct cctccgccac ctctggggcg gaggcatgcg cgcgggaccg gcccttggac
    17761 ggtccagtgc tacagcttgc tccgctggtg gtgctgttgc ggtcctcctg gctgtcgtat
    17821 tgggaggtgg cctcgtggtt cgtgttgtgc actgagttcg tttccatggg gtgtgggagt
    17881 tcggtgtggg cgggcgttgc ggcggcatgg tccgtgtccg ctggtcggtg ggggacgcgc
    17941 agggagccat gcggcgctcg gcctctgacc gtggttcgca gcgcgggtcg aggctgctcg
    18001 tccggaggtc ggtctgacat tgcgagggtc ggggaccggg gaccgggcgg ggtcggtccc
    18061 ctgatggttg gtcctgcggc cgggggcccg gccccgtttc ggtgccgggg tggtcttggt
    18121 ccccaacgga tcggtccccg gatcggtgtg tcgcagggtc ctggtcccca ggttgtcggg
    18181 gaccgagtgt ggacggtccc cgatgctccc ggtccccggg ggatcgggga ccgggagcgt
    18241 cggtggcgcg ctgagcagcg gttttgcagg tatcggtgct ggatccgcga tgctgctggc
    18301 cttggggacc gtgctgtcac ggtcccttct ctcgatgttc gcgcggtccc cggtccccgg
    18361 tttcggcgcg ctcggtcccc gtgcgggttc ccgtcgggga ccgcgtcggg acggtccccg
    18421 acgggaccgg actgtcgagc tgtcgctggg gaccggggac cggggaccgt gtggagccgt
    18481 cggctgaggc ggtctcgtgc gtgcatccga tcgggggacc gacgggctgc cggtcagccg
    18541 accttgccct gctctccctc ctcgcgtgcc atgcctcccg gggcagagac atgtcagtgg
    18601 cggccggcgc tggggcggcg cgcgaccacc tactgcacga gccgcgacgc agctgagggc
    18661 cggcgcccac ggatgctcgc gcgatggcca ccgacatccg gtgggcgatc agccgccacg
    18721 ccggctccag cgccgctcgg gccacggagg cgactgcgcg acgttccaag gcacggcggg
    18781 ttactccgtt tccgctggtt cgtcgagggc ggcgaggagg gctgcggtgt gtgcttcggc
    18841 gcgagtcatg ccgcccgccg aggttgaggt gggggcgccg ccgggcaggg cgcgcagatg
    18901 cgggtgcccg ggggtggggg tgcgttcgcg ggcgatccag gcgcgccagt cggcggccca
    18961 cgcggcggcg gtgcgcggta taccgaccgc tcggtaggag cgccacttct cgtcggcggt
    19021 gcgcagggcg tgatcgccga gccgggcgag gtgcccctgg tgctgcagcc aggcccgggt
    19081 gtcctcgtcg atctgccatt gggtggcggg gatgagtggg gcagagctgc tgctcttacc
    19141 gttcaccttc ggttctctac tgttctgggg gtcggaatcc gtgctccttg agggttgggt
    19201 tccgtgcccc ctgggggttg gattccgttc cccggggtcg gattccggtc ccccttcgaa
    19261 gggggcggcg gggtcggatt ccgtcccctc gggggttgga atccgttccc cctggagagt
    19321 cggattgcgg ccctcgtcga tggcttggtc ggcgaggaat cgggcggcgg ccgggaggcg
    19381 gtagtaggtc tctccacggg gccccttgcg gcccatcagc tgctccagct cgccggtggc
    19441 gatcagcttg gccagggcgt cgcgcacggc ggtacgggag gcgttcgcgc gcttcatgag
    19501 ggtgggcagg gacgcgtagg cgacgcagcc gcggtctacg cagcggtcgg cgatcagggt
    19561 gaggaccatg cgggcggtgc ccttgctggt gctgtggtcc cacacccatt cgcgggcggc
    19621 gtggctcatc gggtgatggc tccttggtcg ggggcggtga ggcgcggcga gccctcttcg
    19681 ggcgaagcgc aggagggggt ggcacacggt tccggcccga acgtgccgga agggccgcgg
    19741 aggcaggaag gtgggttcgc ccggccccgg attggcatgc cggaatctct catgccctga
    19801 gctggcagcc cggggccgat atccaagaca gccacaacga cgtcgcgcaa gtccagtatt
    19861 ccgctgggaa atgaggaagc tggcagcgaa cacattctga gcggcggtcc caaaacgata
    19921 gccccggatt ggccgttgcc gaaatcagtt agcaccaggg cgagccagaa ggatcacatc
    19981 gagaaaccgt tgacccccgt tgcggcgcaa gctacaacac aacaccacac gtcagagagc
    20041 tttcggatgg ccggtgcagc aacatccgcc gacgcccaga acccagttcg gaggttcatt
    20101 cacggtcgcg cgccgggcga aacatagacg acgatcggcc gttaaccaaa ccggcgatca
    20161 cgagctataa ctgagcccat ggcagtgcgt ccccttgaaa tcggtctggc aggaacccgg
    20221 accacccgca atatcgaaat tctccgcacc gaacgcggtc ctgcttagcc tcagctggcc
    20281 gcccgcgtca ccgccctcgg ccgacccatg accgccactg cgctctcccg catcgaacgc
    20341 aaggcccgac gctgcgacat cgacggctgg tgatcatgga gacggcccgg acgctccagg
    20401 aacactggcc gaaggtgtcg gcgccggaag ggcgttggca gggggcgact cgcaccgctg
    20461 tgtcccatcg gtaggatctc gggcctactc ggcacttgac ggacctgcgg gggaagcaga
    20521 ccgatggcag ctatcgtgat cgtgcacggc gtcggaaacc agtacctggg caagcacacg
    20581 cttcacagca gcgtcgcgcc cgcgctgctg gacggcgtac gcctggccga cggcccgccg
    20641 ctcaagcccg acgacgtcga agtcgccttc tacggacact ggttccgacc gcccggcacc
    20701 acctccgtca agggcgaacc ggcgtacgcc caccgtgatg tgatgggtcc gttcgaaacc
    20761 gaactgctgc tggccctctg gcgagaggcg aaccggctgg agccggaccg ggtgcccgcg
    20821 ccggacctga cgtgcacggc ggacaccggc acggcacgga gcaaggccgc cgtaccgtac
    20881 tcggtgcaac gagcgctgta cgcgctcagc aggtcgtcgt tcctggcggg cgtggccgac
    20941 cgcttcctga tcggggtcct gaaacaggta caccgctatc tcaccgacga cctcgtccgc
    21001 gacagcgttc agcgggagat cgccgactgt gtgacccccg agacccgcgt gctcgtcggc
    21061 cactccctcg gatccgtgat cgcgtacgag gcgctgtgcg cacaccccga gtggcccgtc
    21121 acggccctgg tcaccatcgg ctctccgctc ggcatcccca acctggtctt cgaccggctg
    21181 cgaccggcac cccgggcggg ccggggcgcg tggcccggtg gggtgcgccg ctggaacaac
    21241 ctgtgcgatc gacgggacgt cgtcgccctc gccaaacggc tcgcccctct gttcccggac
    21301 ccgacgagaa cggtcgacga cgtcctcgtc gacaacggct ggcgggccca cgccctcgaa
    21361 caccacctca cggccgccga aaccggcgct gccatagccg agggcctggc cgggggagcg
    21421 cgatgacaga caccgccgta ccccgttggg cgcttgtcgc gggaaaccgg gagtacgaat
    21481 acctcgagca actgccgtac gtcgacctgg acctcgacgt catggccgga ctgatgtccc
    21541 ggctcggtta cggcaccgcg cgccggatct ccggctgcac gaccgaggga ctgcgcacgg
    21601 agctggccga ctgggccacg accgagtccg acgacggccg cggcgagggc actctggtcc
    21661 tctactacac aggccacggc gaccgcagcg ccgacgaccg ccactacctc ctgtgcaggg
    21721 acagccgccc cgggcgcctc aagggcacgg ccctggccac cgaggacgtc gtcggcatcg
    21781 tgacggagac cggcctgcgg cgcctattgc tgatcatcga cacctgctac gccggtcagg
    21841 gctccgtcga tgccgtccgt gaactggccc gcaacctgac cgcgacgcgc gaggccgacc
    21901 agcaccgtct cacgtcgttc tccgtcatcg ccgccacccg gccactcgaa ctcgccgcgg
    21961 acggcgcctt cgccaagtcc ctgcgcgacg ccgtcgacga cccgaccctc ggtggcccgc
    22021 gccagcgcaa gctgttcgtg gagcaggtcg tcgaccgggt caacgaactg ctcgtcgaac
    22081 gcagccccgg ccagcacgcg accttcggca ccctgtgcgg tgacgacgtc ccgctgttcc
    22141 cgaacccccg gtacgcaccg gacgctccgg cggagggcgt cgacctctcc gagcaacgcg
    22201 tcgcgctcag cgcggaaggg cgacggcggc gggaggaact gttcagccac ttcggaccac
    22261 ggggtcgggg aacgcaacgg gccgccgagc gggggacata cttcaccggg cggcatgaag
    22321 cgttgcgtgc cctgatggcg tgggtgaagg ggaaggagga tgacgacgag cacctcatgc
    22381 acagaatggt gactgtggac atccggccgc gccggacggt gttcagtgcc cagcgccaac
    22441 tcgtggtgac cggcagccca ggcgtgggca agtccgcact gctcggccgc cttgtcctgg
    22501 aggactccga ggtcacccgt cggcccgacc tgagcgaact ga
//
