LOCUS       NZ_CP050976            11318 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6928449
            Orig. end                         :: 6939767
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11318
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="26"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11318
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11318
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6318](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6318)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            518..1270
                     /gene="hemQ"
                     /locus_tag="HEP86_RS30890"
                     /old_locus_tag="HEP86_30890"
     CDS             518..1270
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="HEP86_RS30890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30890"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_042160056.1"
                     /transl_table=11
                     /translation="MTDASTPASAPQTPAAPEKVPHAGKKAKDLNEVIRYTLWSVFKLR
                     DVLPEDRSGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQEAY
                     NLFRRTRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEQPRAYVSVYPFVRSYEWYLL
                     PDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGS
                     EARMHVREEIPFYTGRRKPVAELVAGLA"
     gene            complement(1371..1592)
                     /locus_tag="HEP86_RS41025"
     CDS             complement(1371..1592)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /locus_tag="HEP86_RS41025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_348772283.1"
                     /transl_table=11
                     /translation="MELQRRLPGSSLVTENGAGTHGVAFNDNACVNKYAEAYLLHGKIP
                     AHKVYCAPRAEPLPGQQATRAQHALPGK"
     gene            complement(1499..3058)
                     /locus_tag="HEP86_RS30895"
                     /old_locus_tag="HEP86_30895"
     CDS             complement(1499..3058)
                     /NRPS_PKS="Domain: Abhydrolase_1 (92-292). E-value: 2e-16.
                     Score: 52.3. Matches aSDomain:
                     nrpspksdomains_HEP86_RS30895_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="HEP86_RS30895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30895"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_248001375.1"
                     /sec_met_domain="PF00561 (E-value: 2e-22, bitscore: 78.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLLVTALATAPAGGATGAAAAPPAPVPHRIEFGR
                     CAAVEHLPSTVECGRLTVPLDYARPDGKKIHLTVSRIRATARGERQGALVFNPGGPGAS
                     SMEFPLYGALPKWRKVARAYDFVGYAPRGVGRSAPLSCQNPKEFAKAPTDSRMHPDAAF
                     KRKKRTEARAYARGCVRRAGADLPYYNSVNNARDLDMLRAALGEKRLTFMGASYGTYFG
                     ALYATLFPGHVRRMIFDSVVNPAPHKIWYRNNLDQDIAFERRWGDWLGWVARHNAQYHL
                     GTTKKAVQQAYNKAAGKLRRTPLNGKIGPGQLQSAFLKTGYNDAFWPMRAEALAGYLHG
                     NPKPLIAQALPRDTGAAADENSNAVYTAVECNDAPWPHDWRTWNRDNTEIARIAPFETW
                     DNAWMNLPCAYWPDRPASAAAELDDAVRRADHPATGRDLADVAGHVARTQVDEALHGRR
                     GPLDVHTEPGALPPVLLLAAERDAATPYRGRWSSSAGCPAPRWSPRTARAPTASRSTTT
                     PV"
     aSDomain        complement(2183..2782)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEP86_RS30895_Abhydrolase_1.1"
                     /evalue="2.00E-16"
                     /label="HEP86_RS30895_Abhydrolase_1.1"
                     /locus_tag="HEP86_RS30895"
                     /protein_end="292"
                     /protein_start="92"
                     /score="52.3"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMEFPLYGALPKWRKVARAYDFVGYAPRGVGRS
                     APLSCQNPKEFAKAPTDSRMHPDAAFKRKKRTEARAYARGCVRRAGADLPYYNSVNNAR
                     DLDMLRAALGEKRLTFMGASYGTYFGALYATLFPGHVRRMIFDSVVNPAPHKIWYRNNL
                     DQDIAFERRWGDWLGWVARHNAQYHLGTTKKAVQQAY"
     gene            complement(3143..3766)
                     /locus_tag="HEP86_RS30900"
                     /old_locus_tag="HEP86_30900"
     CDS             complement(3143..3766)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HEP86_RS30900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30900"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_348772255.1"
                     /transl_table=11
                     /translation="MAYLSGGPHRLADVVLVLMAQQRRLLLADTGWATVVDPVGKDAVE
                     RAALTAIGPDGQRRIPAIRDALVGDDAVRQVADRLVTDGLAVPAAVRPGVGSAVRLVQG
                     AALLVLLTAAASYWMAPAGTDAGLLLAWFSLPLILTLGTWAVARFELHPYSDWATTAGE
                     RQLPGVGHSPAAPATPASLVTLALRGPAALTDPALRAALHSSGA"
     gene            complement(3988..4995)
                     /locus_tag="HEP86_RS30905"
                     /old_locus_tag="HEP86_30905"
     CDS             complement(3988..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175676.1"
                     /locus_tag="HEP86_RS30905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30905"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_168481867.1"
                     /transl_table=11
                     /translation="MSYPQAVYAGIAASSLLLIVGALSVRLRTPSVPRTDVLAHDVWEM
                     AFLAGGPGRVVDAALAGMHEDGRLAVGGPGVVTVRQALARDAVEAAVLDAIARTPGGAL
                     AALRATAMRSPAVQAVGDGLAARGLLRRPQLGRGWRRWAGVQMTVCGALFFVGVLLAVT
                     GSGGGDGFAVPPVVTTAPALIGGLVVASVCRKAVTRRITKAGSFALHTAKVAQAHAGGG
                     GVWQPAGLVVALGGTALIADESLRQQFEEAQRAAAGSGSASSGSSASDTSGGSSGSDGG
                     SWCGSSSSGSGCGSGGSSCGGGSSCGGGSSCGGGSSCGGGGCGAAEPGGHLPAC"
     misc_feature    complement(4974..4990)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            complement(5001..6318)
                     /locus_tag="HEP86_RS30910"
                     /old_locus_tag="HEP86_30910"
                     /pseudo=""
     CDS             complement(5001..6318)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984740.1"
                     /locus_tag="HEP86_RS30910"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30910"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 9.3e-95, bitscore: 315.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAELPGVDWVEVVAENICPGHLPYALEE
                     LRERGTTVIPHGVSLGLGGADRPDPGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PRMEAGHLLPVPRTRDALDVLCANVRIAQDALPVPLALENIAALINWPGEELTEGQFLT
                     ELVERTGVRLLIDVANLHTNHINRGEDPETALDELPLDALAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAALCDRTTPPGVLLERDDDFPGGGELAGELDAIRTVLKEARHDRAA"
     gene            <6651..>7013
                     /locus_tag="HEP86_RS30915"
                     /old_locus_tag="HEP86_30915"
                     /pseudo=""
     CDS             6651..>7013
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_4 (36-120). E-value: 2.2e-08.
                     Score: 26.1. Matches aSDomain:
                     nrpspksdomains_HEP86_RS30915_Aminotran_4.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970024.1"
                     /locus_tag="HEP86_RS30915"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="HEP86_30915"
                     /product="aminotransferase class IV"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHGRTLPDDRVRAYLRTALRAGPADLSLIATVYSPAGEFTVTGPD
                     VEPELLVRTAPPATGPEGPLALAAVEHERELPAVKHVGEVAKTYRLRQAVEQGCDDAAF
                     VDRRGRLSEATIWNLAF"
     aSDomain        6759..7010
                     /aSDomain="Aminotran_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEP86_RS30915_Aminotran_4.1"
                     /evalue="2.20E-08"
                     /label="HEP86_RS30915_Aminotran_4.1"
                     /locus_tag="HEP86_RS30915"
                     /protein_end="120"
                     /protein_start="36"
                     /score="26.1"
                     /tool="antismash"
                     /translation="GEFTVTGPDVEPELLVRTAPPATGPEGPLALAAVEHERELPAVKH
                     VGEVAKTYRLRQAVEQGCDDAAFVDRRGRLSEATIWNLA"
     gene            complement(7159..7920)
                     /locus_tag="HEP86_RS30920"
                     /old_locus_tag="HEP86_30920"
     CDS             complement(7159..7920)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088892.1"
                     /locus_tag="HEP86_RS30920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30920"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_168481869.1"
                     /transl_table=11
                     /translation="MTSTETAEPSGNSAASPFAHAETDRDAAPQFVLPLVVRIEREAPP
                     ARTDALETAARAVLVLLSDERSAGDGEWAAAVRDWQDARIRKVVRRARRAEWRRATALP
                     GITVTGHEAEVRVFPPVPLDGWPKDLARLQVSGTELDDPQPPGAPADGPVLWFNPALEM
                     SAGKEMAQAGHGAQLAWWELSEEERASWRAAGFPLSVRSAEPAEWDALTRSGLPVVRDA
                     GFTEIAPGSCTVVADHPALRRALSAEGDGRF"
     gene            8146..9066
                     /locus_tag="HEP86_RS30925"
                     /old_locus_tag="HEP86_30925"
     CDS             8146..9066
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="HEP86_RS30925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30925"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_248001376.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGVRGPWVLREVDLDVPARALLRVEGTNGSGKSTLL
                     RLLAGIDRPSTGRITGRPRTAYVPERFPVALPFTAVGYLTHLGRIHGLPGPEATRRATE
                     WLERFGAAGHARTRLAELSKGTSQKVAVAQALVAEPELLVLDEAWTGLDGGAREVLDRA
                     VAERVADGGTVVFVDHDPQRLAGAADARYRVVDGRLLTHEESGPTAGMTAAAGPRVLIE
                     AEGAAGRLPDDGAPRLPGGPEIAPGPDGTTRLTVPAAHSDALLRALLTARPPWHIRSVT
                     AVPPPHAAALSATDDSPAPHEAPLP"
     gene            9063..9731
                     /locus_tag="HEP86_RS30930"
                     /old_locus_tag="HEP86_30930"
     CDS             9063..9731
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088894.1"
                     /locus_tag="HEP86_RS30930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30930"
                     /product="ABC transporter"
                     /protein_id="WP_168481874.1"
                     /transl_table=11
                     /translation="MIALLRYQTALLIGSHRWLPPVILYAAWLAIGVQSGEPILDAFGY
                     AAGGLLPAAVWLTRVCVTNEPPAARNCAAAAAGPGRVHLACVLTAAVTGLLLALVGTAV
                     VVLLSDPRSTDLRVAVPVPAAAFAGLLAALVCLLMGIAIGALSNWPVLRSTGWSIPTGL
                     LAALLLLVLAASPANAAVSGLVTGSVHGTINTPWLPLAATGLLAAAATAVACAISSRRG
                     "
     misc_feature    complement(10136..10148)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
ORIGIN
        1 tggcgctggt caccatggcc ttccgccgcg ccgacctgga ccgggtgccc ggcggcagcg
       61 gcttcctcgt accgcccgtc gacggccacc gcatcaaggc ctcgaccttc gccagccaca
      121 aatggggctg gatcggcgcc gccgaccccg acctcttcgt gctgcgcacc tccctcggca
      181 ggtaccagga caccgccgac ctccagcgcg acgactccga actggtcggc ctctcgctcg
      241 ccgacctcgg cgcggcggtc ggcctgaccg cccgcccggt ggcgaccacg gtcacccgct
      301 gggaaggcgg actgccgcag tacgccgtcg gccatctgga gcgggtcgcc gggatccgcg
      361 cggccctcgc ccggatgccg ggcgcgctgc gggtgtgcgg cgcggtctac gacggcgtcg
      421 gcatcccggc ctgcatcggc tcggcgcgcc gcgccgcgga cgaactgctc ggcaccctga
      481 cgccggacgc cgtgcccgcc gagtgagaat ggaccacatg acagacgctt ccactcccgc
      541 ttccgcgccg cagacccccg cggcgcccga gaaggtgccg cacgccggca agaaggccaa
      601 ggacctcaac gaggtcatcc gctacaccct ctggtccgtg ttcaagctgc gcgacgtcct
      661 cccggaggac cgcagcggct acgccgacga ggtcgaggag ctgttcgcgc agctcgcggc
      721 caaggatgtg gtggtccgcg gcacctacga cgtgtccggt ctgcgggccg acgcggacct
      781 gatgatctgg tggcactcgg agagctcgga cgccctccag gaggcgtaca acctcttccg
      841 ccggacccgt ctgggccgtg cgctggagcc ggtgtggtcg aacatggcgc tgcaccgccc
      901 cgccgagttc aacaagtcgc acatcccggc cttcctcgcc gacgagcagc cgcgcgccta
      961 tgtgagcgtc taccccttcg tgcgctccta cgagtggtac ctcctccccg acgaggaccg
     1021 ccgccggatg ctggccgacc acggcaagat ggcccgcggc ttcccggacg tgcgcgccaa
     1081 caccgtgccg tccttctcgc tcggcgacta cgagtgggtg ctggccttcg aggccgatga
     1141 gctctaccgc atcgtcgacc tgatgcgtca tctgcgcggc tccgaggcgc ggatgcacgt
     1201 ccgcgaggag atcccgttct acacggggcg gcgcaagccg gtcgccgagc tggtcgcggg
     1261 tctggcgtaa cgggacgcgc gggtccggcc gggcgcgtcc ggccgaggtc ccggacaccg
     1321 caccgcgggc cgccccctca gggacggccc gcggcgctgt gcggggtcgg ctacttcccg
     1381 ggcagggcgt gctgggccct ggtcgcctgc tggcccggca gcggctccgc gcgcggtgcg
     1441 cagtagacct tgtgcgccgg gatcttcccg tgcagcagat acgcctcggc gtacttgttc
     1501 acacaggcgt tgtcgttgaa cgcgacgccg tgggtgcccg cgccgttctc ggtgaccagc
     1561 gaggagccgg gcagccggcg ctggagctcc agcgcccccg gtacggcgtc gccgcgtccc
     1621 gctcggccgc cagcagcagc accggcggca gcgcaccggg ctccgtatgg acatcgagcg
     1681 gaccacgccg cccatggagc gcttcatcga cctgggtcct ggccacatgg ccggcgacat
     1741 cggccaggtc ccgcccggtg gcgggatggt ccgcgcggcg caccgcgtcg tcgagctcgg
     1801 ccgcggccga cgccggccgg tccggccagt aggcgcacgg cagattcatc caggcgttgt
     1861 cccaggtctc gaacggggcg atccgggcga tctccgtgtt gtcccggttc caggtccgcc
     1921 agtcgtgcgg ccagggcgcg tcattgcact ccacggccgt gtagacggcg ttgctgttct
     1981 cgtcggcggc ggcgccggtg tcccggggca gggcctgggc gatcagcggc ttggggttgc
     2041 cgtgcaggta ccccgcgagc gcctcggcgc gcatcggcca gaaggcgtcg ttgtagccgg
     2101 tcttcaggaa cgccgactgg agctggccgg ggccgatctt cccgttgagg ggtgtgcggc
     2161 gcagcttgcc cgcggccttg ttgtaggcct gctgcaccgc cttcttggtg gtgccgaggt
     2221 ggtactgcgc gttgtgccgg gcgacccagc ccagccagtc gccccagcgg cgttcgaagg
     2281 cgatgtcctg gtcgaggttg ttccggtacc agatcttgtg gggcgcgggg ttgaccacac
     2341 tgtcgaagat catccgccgt acgtggccgg ggaagagcgt cgcgtagagc gcaccgaagt
     2401 acgtcccgta cgaggcgccc atgaacgtca gcctcttctc gccgagcgcg gcccgcagca
     2461 tgtccaggtc acgggcgttg ttgacggagt tgtagtacgg caggtccgcg ccggcccggc
     2521 ggacacagcc gcgggcgtac gccctggcct ccgtccgctt cttgcgcttg aaggcggcat
     2581 cggggtgcat ccggctgtcg gtgggtgcct tggcgaactc cttggggttc tggcacgaca
     2641 gcggcgcgga gcggcccaca ccccgtggcg cgtagcccac gaagtcatag gcacgggcca
     2701 ccttgcgcca cttgggcagc gccccgtaga gcgggaactc catgctggat gcgccggggc
     2761 cgccggggtt gaagaccagc gcgccctgcc gttcgccgcg cgccgtggcg cggatccggc
     2821 tgacggtgag gtggatcttc ttgccgtcgg ggcgcgcgta gtcgagagga acggtgagtc
     2881 ggccgcactc gacggtcgac ggcagatgct ccaccgcggc gcagcggccg aattcgatgc
     2941 ggtgcggaac gggtgcgggt ggcgcggcgg cggcccccgt ggcgccgcct gccggggcag
     3001 tggcgagggc ggtcaccagc agcgacccga tggtgccgta caacgcaagt gctctcactg
     3061 gccgtccctt cgtgtgtgca tacaaaaggg atagttgggg cggcgattgt cgttgtaaag
     3121 cacctggtgc cggtgtcgga ggctatgccc cggaagagtg gagcgcggcg cgcaaggcgg
     3181 gatcggtgag ggcggcgggg cctcgtaggg caagagtgac gagggaggcg ggcgtggcag
     3241 gggcggcggg ggagtggccc accccgggca gttgccgctc gccggcggtg gtggcccagt
     3301 cgctgtacgg gtggagctcg aagcgggcca cggcccaggt cccgagggtg aggatcagcg
     3361 gcagcgagaa ccaggccagg agcagcccgg cgtccgtgcc ggccggtgcc atccagtagg
     3421 acgcggcggc ggtcagcagc accagcaggg cggcgccctg caccaggcgg acggcgctgc
     3481 cgacgcccgg acggaccgcg gccggcaccg ccagcccgtc ggtcaccagc cggtcggcca
     3541 cctgccgcac cgcgtcgtcg ccgaccaggg cgtcccgtat cgccggtatg cggcgctgcc
     3601 cgtcggggcc gatggcggtg agggcggcgc gctcgacggc gtccttgccg accgggtcga
     3661 cgacggtggc ccagccggtg tcggcgagca gcagccgccg ctgctgggcc atcagcacca
     3721 gcaccacgtc ggcgagccgg tgcgggccgc ccgacaggta cgccatctcg tacagggtca
     3781 gaccggggcc gcggcgcggc ggccgcgtcc gggacggtct cgggagccgg ccgcggggcg
     3841 ggcccggggg tggcgcgggc cgcggcgacc gccgcacggc agagcagcgc acaggacagc
     3901 accgccgcgg aacaggcgac gaggaagaag agcagccaca tgtcggagtt tttatgcgat
     3961 gtgccgcaac ggccgccaca aaatttctca gcatgcgggc aggtgtccgc cgggttcagc
     4021 cgccccgcag cctccgccgc cacagctcga cccgcccccg cacgacgaac cgcccccaca
     4081 gctcgacccg ccgccgcacg acgagccgcc gctgccgcac ccggacccgc tgctggagga
     4141 gccgcaccag gagccaccgt cgctccccga gctgcccccg gaggtatcgg acgcggagct
     4201 gccggacgac gcggagccgc tgcccgccgc ggcccgctgc gcctcctcga actgctgccg
     4261 cagcgactcg tccgcgatca gcgccgtacc gccgagcgcc accaccagcc cggcgggctg
     4321 ccagaccccg cccccgcccg cgtgtgcctg cgccaccttc gcggtgtgca gggcgaacga
     4381 ccccgccttc gtgatccgcc gggtgaccgc cttccggcac accgacgcca cgaccagacc
     4441 gccgatcagc gcgggcgcgg tcgtgacgac cggcggcacc gcgaacccgt cgcccccgcc
     4501 ggacccggtg accgcgagca gcacacccac gaagaagagc gccccgcaca ccgtcatctg
     4561 gacgcccgcc caccggcgcc agccccggcc gagctgcggc cggcgcagca gcccgcgggc
     4621 cgccagcccg tcgccgacgg cctgcaccgc cgggctgcgc atcgccgtgg cccgcagcgc
     4681 ggcgagcgcc ccgccgggcg tccgggcgat cgcgtccagc accgccgcct cgacggcgtc
     4741 ccgggcgagc gcctggcgca cggtcaccac accggggccg cccacggcca gccggccgtc
     4801 ctcatgcatc ccggcgagcg cggcgtccac cacccgcccg gggccgccgg ccaggaacgc
     4861 catctcccac acgtcatggg ccaggacatc ggtccgcggg acggacgggg tgcgcagccg
     4921 caccgatagg gcgccgacga tcagcagcag cgacgaggcg gcgatccccg cgtatacggc
     4981 ctgggggtag gacatcgcgg tcacccccgt cgccggaaga gccgcagggc gcgcaccagc
     5041 cgggccacgg gccgcgcgga cggcggcttc gggcccgcac gatcccgcca ccagcgcgtc
     5101 aactcccgcc gcgcgaccgg gtcttcgggc cgcccctggg ccagcaggta cgccgcgaag
     5161 tccagggcgt cctggcggta gccgccgctc atgggccggt tccgggcatg gtccgcgaag
     5221 gcggcccggt agtcagtgcc gaggatctcc ggcagttcgg gcgcgacctt ggcgaccacg
     5281 tcggcccgct tggcggccag cgcccggctc tgcacccgca gccgggcgtg gtcgaaaccc
     5341 tcgggggcgg gcgtcccggc gaccagcgcg gacagcagcg cggtctgcgc cagggccagc
     5401 cgccggcggg cgtctcgggt gtcggtgggg ctgtccactc cggcctccac tacggacgcg
     5461 atgtccggcg ccgcctcaag ccgcccggtc atggcgggcc tccttcagca cggtgcgtat
     5521 cgcatcgagt tcaccggcca gttccccgcc tccggggaag tcgtcgtccc gctccagcag
     5581 caccccgggc ggggtggtcc ggtcacacag cgcggcgagt acgtccagga cgggccgggt
     5641 caccggatgg gcgtggctgt cgtgccacac cccgtcgcgc tccaccccgc ccgcgacatg
     5701 gacgtaggcg agggcgtcca gcggcagctc gtcgagggcg gtctccgggt cctcgccccg
     5761 gttgatgtgg ttcgtgtgga ggttggcgac atcgatcagc agccgcaccc cggtccgctc
     5821 gacgagctcg gtcaggaact gcccctcggt cagctcctcg cccggccagt tgatgagcgc
     5881 ggcgatgttc tccagggcca gcggcaccgg cagggcgtcc tgggcgatcc gtacgttcgc
     5941 gcacagcacg tccagcgcgt cccgggtccg cggtaccggc agcagatgcc cggcctccat
     6001 acggggcgag gcggtgagcg gaccgcccgc ccgtacgaag gcgatgtgct cggtgaccag
     6061 cggtgcgccg agggcttcgg cgcgctcggc gagcgcggtc agccggccgg ggtccggacg
     6121 gtcggcgccc ccgaggccga gcgagacccc gtgcgggatc acggtcgtac cgcgctcacg
     6181 caactcctcc agggcgtacg gcagatggcc cgggcagatg ttctccgcga ccacctcgac
     6241 ccagtccacc ccgggcagct ccgcgatctc cgcggcgatt tccggccgcc agccgattcc
     6301 cgtacccagg cgctccatgg ctccccctct cgctccgtgg tgtcgtactc atgccgcgtg
     6361 gagcccggcc gaaccctgca ccggccgagt tcagaggttg atttgagctt caccggcgca
     6421 gcggcggtgc tgcgcggtgc cgcggcgggc tccactctgc tgcaccgcgc tgaccggcgc
     6481 gaacccgccc cgacggtggc ctgatcggac cgggtgccgg ccgggtggcg ccccggcccg
     6541 ctcgcctctc agccggtgcg cggcagcgcc ccacgggtgg gaccgatggg cgactgcgcc
     6601 aagtagccgg cgagagtgcc gcgggtgcgt cgaagggtgg ccatctgcgc gtccatgggc
     6661 ggacactgcc cgacgaccgg gtccgtgcct acctgcggac ggcgctgcgg gccggcccgg
     6721 ccgacctctc gctgatcgcc acggtctact ccccggcagg cgagttcacc gtgaccgggc
     6781 ctgatgtgga gcctgagctg ctggtccgta cggcaccgcc ggcaaccggc cccgaggggc
     6841 cgctggccct ggcggcggtc gagcacgaac gggaactccc ggccgtgaaa catgtcggcg
     6901 aggtggccaa gacgtatcgc ctccgccagg ccgtcgaaca gggatgtgac gatgcggcct
     6961 tcgtcgaccg gcggggccgg ctgagcgagg ccactatttg gaacctggcc ttctcttcgc
     7021 tgccggtgtg aggggcgggg cggggcgggc gtggtcctcc ccgcagggaa accgcgggcg
     7081 ggcaccgcac gcccatgccg ctcccgtatc gcctcgggac gcggtgacca cctacggccg
     7141 tcgtacgcgt gggagaggtc agaagcgacc gtccccctcg gccgacaggg ccctccgcag
     7201 cgcggggtgg tcggccacga ccgtgcagga acccggcgcg atctccgtga acccggcgtc
     7261 ccgcaccacg ggcagaccgc tgcgggtgag cgcgtcccac tccgcgggct cggcggaccg
     7321 caccgacagc gggaacccgg ccgcccgcca ggacgcccgc tcctcctcgg acagctccca
     7381 ccacgccaac tgcgcgccgt gcccggcctg cgccatctcc ttgcccgccg acatctccag
     7441 cgcggggttg aaccacagca ccgggccgtc ggcgggcgcc ccgggcggct gcggatcatc
     7501 cagctcggtg cccgagacct ggaggcgcgc caggtccttc ggccagccgt ccagaggcac
     7561 cggcgggaag acccgtacct cggcctcgtg gccggtgacc gtgatgcccg gcagcgccgt
     7621 cgcccgccgc cactcggcgc ggcgcgcccg ccgcaccacc ttccggatcc gggcgtcctg
     7681 ccagtcccgc accgccgcgg cccactcgcc gtccccggcc gaccgctcgt ccgacagcag
     7741 caccagcacc gcccgcgccg ccgtctccag cgcgtccgta cgggccggcg gggcctcccg
     7801 ctcgatccgg acgacgagcg gcagcacgaa ctgcggcgcc gcgtcccggt cggtctctgc
     7861 gtgcgcgaag gggctggcgg cgctgttgcc ggacggttcg gcggtctcgg tgctggtcac
     7921 ccgcccagtc tgccagccgc caccgacagc gccccggccc gtatccggcg ctccggccct
     7981 cgccgggcca cggcgagggc caactccgtc tgtccggcac gcggttgggg cgcgtacggt
     8041 ccggcgctgg acgcgccccc gggcaggcac ccggcggccg ctttggctac cgtcgggggc
     8101 ctggagcggc ggggccgcca ggaggcagcg gaggtgccgg gacgtatgag gctggacggg
     8161 gtggggcggc ggtacggggt gcgcggcccc tgggtgctgc gggaggtgga cctcgacgtg
     8221 cccgcccgcg cgctgctgcg cgtcgagggc accaacggca gcgggaaatc caccctgctg
     8281 cgactgctcg ccggaatcga ccggcccagc accggacgga tcaccggccg cccgcgcacc
     8341 gcctatgtgc ccgaacgctt cccggtcgcc ctccccttca ccgccgtcgg ctacctcacc
     8401 cacctcggcc gcatccacgg tctgcccgga cccgaagcga cccgccgcgc caccgagtgg
     8461 ctggaacgct tcggggcggc cgggcatgcg cggacccggc tggccgagct gtccaagggg
     8521 acgagccaga aggtcgcggt cgcccaggcg ctggtcgccg agcccgagct gctggtcctg
     8581 gacgaggcct ggacgggtct ggacggcggc gcgcgcgagg tgctcgaccg ggcggtcgcc
     8641 gaacgggtcg cggacggcgg caccgtcgtg ttcgtcgacc acgacccgca acggctggcg
     8701 ggcgccgccg acgcccgcta ccgggtcgtg gacggacggc tgctgaccca cgaggagagc
     8761 ggcccgacgg cgggtatgac ggccgcggcc ggtccccggg tgctcatcga ggccgagggc
     8821 gcagcggggc gtctgccgga cgatggagcg ccgcggctgc ccggcgggcc ggagatcgcg
     8881 cccggaccgg acgggacgac ccggctgacg gtgccggccg cccactccga cgccctgctg
     8941 cgcgcgctgc tcaccgcccg tccgccctgg cacatccgct cggtcaccgc cgtcccgccg
     9001 ccgcacgccg ccgccctgtc tgccaccgac gattcccccg caccccatga ggcgcccctc
     9061 ccgtgatcgc tctcctccgc taccagaccg ccctgctcat cggctcgcac cgctggctgc
     9121 cgccggtcat cctctacgcc gcctggctgg ccatcggcgt ccagagcggc gagccgatcc
     9181 tcgacgcgtt cggctacgcc gccggcgggc tgctgccggc cgccgtatgg ctgacccggg
     9241 tctgtgtcac caacgagccg ccggccgccc gcaattgcgc cgcggccgcc gccgggcccg
     9301 gccgggtaca cctcgcctgt gtactgaccg cggccgtcac cgggctgctg ctggcactgg
     9361 tcggcacggc cgtggtcgtg ctgctgtcgg acccgcgctc caccgacctc cgggtggccg
     9421 tcccggtccc ggccgcggcg ttcgccgggc tgctcgccgc gctggtgtgt ctgctcatgg
     9481 gcatcgccat cggcgcgctg agcaactggc cggtgctgcg cagcaccggc tggagcatcc
     9541 cgacgggtct gctcgccgcc ctgctgctgt tggtgctggc cgcctcgccc gccaacgccg
     9601 ccgtatcggg cctggtcacc ggctccgtcc acggaacgat caacaccccg tggctgcccc
     9661 tcgccgccac cggcctcctc gcggccgccg ccacggcagt cgcgtgcgcc atcagctcac
     9721 ggcgggggtg acgggggaag cccgcacgga aggggagctg acggggaaag cccgcgcgga
     9781 aggggctccc gagctctccc ccgccccctc cccctctccc ccttcggggg gagggggggc
     9841 ggtgggttcg gggtgggttc gggggtgggg ggagcggccc ccgccgcaac gtcagcggag
     9901 tccgggcgac ggcgcagcgt cagaggtacc aggaccccgg cacagcgtca cgggtaccag
     9961 gaccccggca caacgtcagt gatgcggggc ccagtcaccg cgtcagcgcc accagccccc
    10021 gcctcacctc agccatgcca gaggcccctc accgcccctc agtgatgcca aggccccgta
    10081 accgcgaacg tcgtccccgg tgtgtagcag ttgacgtaca tcgtgcggtg gccgggtgag
    10141 aacgtcacgc cggcgaactc gccccactcc ggtttctgcg gggtgccgat gttctgccgg
    10201 ccgcgggcca tcgggtagac ctcgccgcgc cgggtgaggc cgaagacgtg ctgggcgccc
    10261 tcgccgtcct cgcagaccat caggccgccg ctgggtgcga ggcagatgtt gtcgggggat
    10321 tcgccgggca gttggaggtc ggtgtccggg ccgaagacga cgacctgggt cagctgccgc
    10381 ttcttcgggt cgtacttcca gacctgtccg tagtggtcgg ccgccgagcc gtccgcgcgg
    10441 tgggcgaagc tggagatgaa gtagacgcag gagccgcccc agtagcagcc ctccagcttc
    10501 tgggcgtggg tgatgccctg ggggccgaag tcctggaagc ggatgggggt ctgacgggcc
    10561 agcgggtcgg gcacatcgac ccaggtgacg tggtcgaagg tggtgccggg ccgctggatg
    10621 gtggagaggt cgggcacccc ggggacgcgc agcgcctgga ggcggccgcc ggcccggagt
    10681 gaaccgcggc cgcccctcgg cttctcgggc agaaagcggt agaagaggcc gaacggcttc
    10741 tcgaaggcgt cctcggtctc gtagacgatg ccggtgccgg ggtccacggc gatcgcctcg
    10801 tgctggaagc ggcccatcgc ggtcagcggg acggctccgg tgcgccgcgg gtcgtagggg
    10861 tcgacctcga agatgaagcc gtggtccttg gtgtagttct cctcaccggc ccggagttcg
    10921 gtctcctcgc aggtcagcca ggtgttccag ggggtggggc cgcccgcgca gttggtggag
    10981 gtgccggcga tggcgacccg ctcggagagc accttgttgt cccggtcgag tgcgagggcc
    11041 gtacagccgc cctcgccacc ggggtcgtac gtcagatcgc ggatccgggg gacggcctgc
    11101 cgggagttcg tacggttctc gtggttgcgg accaggtggg tgccgccgct gcggccgggg
    11161 aaggccgaca tcccgtcaca gttgctgggg accttgccct ctcccgagtg cagtgggtcg
    11221 ccctcccggg acaggacccg gtagcggaag ccccggggga ggtcgagcag accgtccggg
    11281 tccggcacca gcgggccgta gccgcgctgt cccatgct
//
