LOCUS       NZ_CP050976            10978 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6861998
            Orig. end                         :: 6872976
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10978
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="25"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10978
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10978
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5978](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5978)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            963..1331
                     /locus_tag="HEP86_RS30620"
                     /old_locus_tag="HEP86_30620"
     CDS             963..1331
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEP86_RS30620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEP86_30620"
                     /product="hypothetical protein"
                     /protein_id="WP_168481812.1"
                     /transl_table=11
                     /translation="MVTAGRGGGGGAGVGVGVGAVSGPPGLLRPLHRDLAVGLRRAARR
                     AVRRPLRRAAARRATVRCHHHSPRLPQRDLIAQARHEQRAVVVYLDPAARWPLFHHQLD
                     GGGAAGVLERVGDQFRGQ"
     gene            complement(<1221..1466)
                     /locus_tag="HEP86_RS41020"
                     /pseudo=""
     CDS             complement(<1221..1466)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312159.1"
                     /locus_tag="HEP86_RS41020"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLEPPTPATWRIALPRGATAVPIARAMVRTALQDLRVTADRTTAQ
                     LLTAELVTNALKHTCGAAPIELVVEQRPSGCRIEVHD"
     misc_feature    1670..1690
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            1770..2597
                     /locus_tag="HEP86_RS30625"
                     /old_locus_tag="HEP86_30625"
     CDS             1770..2597
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 2e-50. Score:
                     163.3. Matches aSDomain:
                     nrpspksdomains_HEP86_RS30625_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445228.1"
                     /locus_tag="HEP86_RS30625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30625"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_168481813.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTETWQHLRVTRQDGVATVTLARPDKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RAVRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISDLAEEGRADEAAAALAKRLADGPAMAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSDDYAEFHAAFTEKRAPKWQGK"
     aSDomain        1827..2570
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEP86_RS30625_ECH.1"
                     /evalue="2.00E-50"
                     /label="HEP86_RS30625_ECH.1"
                     /locus_tag="HEP86_RS30625"
                     /protein_end="267"
                     /protein_start="19"
                     /score="163.3"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARPDKLNALTFEAYADLRDLLAELSRERSVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRAVRECPFPVIAAVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISDLAEEGRADEAAAALAKRLADGPAMAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSDDYAEFHAAFTEK"
     gene            2600..4891
                     /locus_tag="HEP86_RS30630"
                     /old_locus_tag="HEP86_30630"
     CDS             2600..4891
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="HEP86_RS30630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30630"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_168484079.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAVLLKRLDPAREITVWERNAPEDTFGFGVV
                     LSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRRLLAVLH
                     RRCRDLGIELRFRTEAPPAAELAGAYDLVIAADGVHSATRTAHADAFRPRLATHHCRYI
                     WLAADFAFDAFRFEIAETEHGVVQLHAYPFARPAGPAAGASTVIVEMREEVWRSAGLDR
                     CDERESAEWCAKVFTDALRGRPLHGNNSSWTAFRTVVNDRWSHGNTVLLGDAAHTAHFS
                     IGSGTKLAVEDALALAACLEEQPDTARALAAYEEERRPVVASTQRAARASLAWFEELAT
                     YLDQPPHQFAFNLLTRSRRVTHDNLRLRDPGFVAAVQDEAGTPPGTPPMFLPFRLGELT
                     LRNRVVVSPMDMYSAADHNGTPGDFHLVHLGARALGGAGLVMTEMVCVSPEGRITPGCT
                     GLYAPEHETAWRRVTDFVHTSAPGTAIGVQLGHSGRKGSTRLMWDGIDQPLPDGNWPLV
                     APSALPYRPGVNQTPHALTEPELATLREQFVRAAESAARAGFDLLELHCAHGYLLSGFL
                     SPLTNHRTDAYGGDLTGRLRFPLEVFDAVRAVWPAGRPMTVRISATDWADGGTTAADAV
                     AMAQAFAAHGADAIDVSTGQVVSDERPDFGRSYQTPFADRIRNTLGVPVIAVGAISSWD
                     DVNSLVLAGRTDLCALARPHLYDPHWTLHAAAEQGYAGPGAPWPLPYQAGSRTPPTGRL
                     DAPKPRLTLR"
     gene            complement(5001..5978)
                     /locus_tag="HEP86_RS30635"
                     /old_locus_tag="HEP86_30635"
     CDS             complement(5001..5978)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="HEP86_RS30635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30635"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_168481814.1"
                     /sec_met_domain="AfsA (E-value: 2e-22, bitscore: 77.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTPYLRDQRHEVGGGSADGIRPSSLTTTVPREYVHRNALS
                     EVFLTNWRRGGADSWVVSAQWPRAHTFYGPLHGMHDPILLIETVRQAGILLSHVAHQVP
                     LDHPIIWQRVRYDLAPQALRTAQHPAEVELHITDRDMVHRGKRLVSALQEFQIRCDGAE
                     LASATLDYSCHSPAVYRRLRGEYSDLALANSRTLPLPEAVRPQLVAREHDRDVVLSPTD
                     RPDRWQLRVDTSHPVLFDHPVDHAPGMLMVEAARQAAQAATPGVTLPVSMECSFSRYAE
                     LDAPTWVQARTTGRGDDDRHQVEVAVEQHGQPVVSAQVSCVPTP"
     misc_feature    complement(5694..5696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6120..6716)
                     /locus_tag="HEP86_RS30640"
                     /old_locus_tag="HEP86_30640"
     CDS             complement(6120..6716)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="HEP86_RS30640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30640"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_248001370.1"
                     /transl_table=11
                     /translation="MRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGAVYFHFTS
                     KDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIAVETTFSE
                     DPLVPYQAWTDIITTMFTEARDSGELLPGVAPDRAAEFFVAAYMGVQLFSRAATNRADL
                     PERVTALWKHTLPGLASPGALSHLDPHGRCVTIPV"
     misc_feature    complement(6327..6329)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6833..6851)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     gene            6944..7971
                     /locus_tag="HEP86_RS30645"
                     /old_locus_tag="HEP86_30645"
                     /pseudo=""
     CDS             6944..7971
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="HEP86_RS30645"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30645"
                     /product="NADP-dependent oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSPTVVPAIPAVHREVRLATQVEGELTARHFTIAEVPVPEPRAGE
                     VLVRNRVMAVTAVMRSQMTGIRLPMPSFVPGKALWGAAVGEVVAAPGGGFTPGELVHHP
                     CGWREFAVVDASRVRSLGSDALPTAAAHLSQGRPPGVPCAGPPRSGRETPCSSVVRRAA
                     WAAWPGSSHGGWAPGGSSAAPARSAKPRGCAPSWATTTRSCAAPGRSSGSCGGPRRAVS
                     MCCWTPWAANS"
     gene            complement(8004..8636)
                     /locus_tag="HEP86_RS30650"
                     /old_locus_tag="HEP86_30650"
     CDS             complement(8004..8636)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="HEP86_RS30650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30650"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_168481816.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALEALQELEDTFTRSTQPLTIAVRDFTTALFSRVESDAVLR
                     AGLQLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGPAGPHATDTEPRRTAQLLTSLV
                     VGLLHLGSEDGTWWDHEGIVDLWALLPAAPGRIPAAVPAPAQLPAAG"
     misc_feature    8884..8898
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    8910..8924
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.22"
                     /tool="antismash"
     gene            9088..9852
                     /locus_tag="HEP86_RS30655"
                     /old_locus_tag="HEP86_30655"
     CDS             9088..9852
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602521.1"
                     /locus_tag="HEP86_RS30655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30655"
                     /product="HAD family hydrolase"
                     /protein_id="WP_168481817.1"
                     /sec_met_domain="HAD_2 (E-value: 2.7e-27, bitscore: 94.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPVRGVRGAGGETIRAAVFDLDGTLANTLPAISKLL
                     IKVASEQGCSVTARQAAAAIGKPPGPAFGRLLGRPEDDSRVQAAISRYRELFAYEVLCL
                     GPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVIGQDMVRRG
                     KPHPEMVLRAAGRLGVAAWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAGVAG
                     TRMCGSFKDVVSVIAAARPRSGVKVGAQQRRR"
     gene            complement(9872..10723)
                     /locus_tag="HEP86_RS30660"
                     /old_locus_tag="HEP86_30660"
     CDS             complement(9872..10723)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088832.1"
                     /locus_tag="HEP86_RS30660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_30660"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_168481818.1"
                     /transl_table=11
                     /translation="MNDPHTQRTPRSLIVTFYGAYGRGEPGAPGGPAAPVPVAALIRLL
                     GALGVDPPSVRSAVSRLKRRELLTPARTATGAAAYGLSDAARQLLDDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLGRLGFGTAAPGVWIAPAQLYEETRHTLERLQL
                     DPYVDLFTGTHAGFAPTADAVARWWDLDAIAAQHRAFLDAQEPVLQRWSRRRSVPPEAA
                     YRDYLLALDAWRRLPYADPGLPSPLLPEDWPGGRAADVFGRLHAKLRDAGAPYAHEAMA
                     GA"
ORIGIN
        1 acctgtcgcc gttcgccccg gagggtgtcg ccggcatcac ctccggcgcc tcgctcgcgt
       61 tcttctcgta catcggcttc gacgcgatca ccacggccgg tgaggaggcc agggacccgc
      121 ggcggaacat ccccgtcgcg atcatggtct gcatcggtgt ggtcaccgtc ctgtactgcc
      181 tggtcgcgct ctccgccatc ggcgcgctct ccgccggcca ggtcggcggc cggcccgccg
      241 cgctgtcgct gatcgtcaac cacgtcaccc actcgacgct cggcggcggg gtgatcgcgt
      301 tcggcgccgt cgtcgccatc gcctcggtcg tcctcgcggt catgtatggc cagacccgca
      361 tcctgatgtc gatggcgcgg gacgggctgg tcccgcggat cttcgagcgg gtctcgcccc
      421 ggacgtccac gcccgtcgcc aacacctgga tcgtcgcggc cgtcttcgcg gtgccggccg
      481 cggtcgtccc gttggacgcg gtgatgaacc tgtcgaccat cgggacgctg gcggtcatgg
      541 cggtggtcaa cctcagcgtg atggtgctcc gccgcaaccg ccccgatctg ccgcgccgct
      601 tccgcgtccc gctcttcccg gtcagcccgg tgctcggcat cgccttctgc ctctatctga
      661 tctacggcac cggcccggcg acctggctcc agttcgccgg gttcctggtg gccggtgcgc
      721 tggtctacac gtgctacgga cgccgtcgct cgcggctcgc gacggggccg cagggcccgg
      781 cggccgacgc ggatgcggtc gacgccgatg tgcctgacgc cgacgcgacc ggtgctgacg
      841 cggccgccgt cagcgtcggc gccgcacctc gggaagggtg aaccagacgg ccttgccgcg
      901 cggcgtcgga cgacagccgg aggccgcgct gaggaccgga tcagcagcag accacgaccg
      961 tcgtggtcac ggcaggccgc ggcgggggag ggggcgccgg ggtcggtgtc ggtgtcggtg
     1021 ccgtctccgg accgcccggc ctcctccgcc cgctgcatcg ggacctggcc gtcggcctgc
     1081 gccgtgccgc ccgccgtgcc gtccgacggc ccctccgccg cgccgccgcc cgccgcgcaa
     1141 ccgtccggtg ccaccaccac agcccccggc tcccccagcg ggacctgatc gcccaggccc
     1201 gccacgagca gcgggctgtc gtcgtgtacc tcgatccggc agcccgatgg ccgctgttcc
     1261 accaccagct cgatgggggc ggcgccgcag gtgtgcttga gcgcgttggt gaccagttcc
     1321 gcggtcagta gttgagcggt ggtccggtcg gccgtcaccc ggagatcctg cagggcggtc
     1381 cggaccatcg cacgggcgat ggggacggcg gtcgcgccgc gcggcagcgc gatgcgccag
     1441 gtcgcggggg tgggcggttc cagcatgcgc ggttcgtccg ttcgcgaagg gccgggtcgg
     1501 cggcctgaga ccggatcggg cctctccacc gtagggagcg cggaggcggg ggatcagagg
     1561 cgagcggccg gggatcggat gacctccgtc atgcggcggc cgggaccttc ggcgggttca
     1621 ccccgacggc cgcggcgggt ggcgggatgc tgtcgtcggc ccgtgtcgta tcgcgttctc
     1681 atgacgacag tcatgaacgg gtgataactt cgaggggcat ccggaacagc atccaccggc
     1741 cgacccggcc aaagggggca ggcagcccga tgagcccgtt cgcaggatcc gcccgcagta
     1801 ccgagacctg gcagcacctt cgcgtgacgc ggcaggacgg ggtcgccacc gtcaccctgg
     1861 cgcgcccgga caagctcaac gcgctcacct tcgaggccta cgccgacctc cgcgacctgc
     1921 tcgccgagct gtccagggag cgttccgtac gcgccctggt gctcgcgggg gaggggcgcg
     1981 gattctgctc cggcggcgac gtcgaggaga tcatcggcgc caccctgggc atggacaccg
     2041 cacagctgct ggacttcaac cggatgaccg gacaggtcgt ccgggcggtg cgggagtgcc
     2101 ccttcccggt gatcgcggcg gtgcacgggg tcgcggcggg cgcgggcgcg gtgctggcgc
     2161 tggccgcgga cttccgggtc gccgacccgt ccgcccggtt cgccttcctc ttcacccggg
     2221 tcggactgtc cggcggcgac atgggcgcgg cctatctcct gccccgcgtc atcggcctcg
     2281 gccacgccac ccggctgctg atgctcggcg acaccgtccg cgcgccggag gccgaacgcc
     2341 tcggtctgat cagcgatctg gccgaggagg gccgggccga cgaggcggcc gcggcgctcg
     2401 ccaagcggct cgccgatggg cccgcgatgg cacacgccca gaccaaggcg ctgctcaccg
     2461 ccgaactcga tatgccgctg gccgccgccg tggagatgga cgccgccacc caggcgctgc
     2521 tgatgaacag cgacgactac gcggaattcc acgccgcctt cacggagaag cgggccccca
     2581 agtggcaggg gaaatagcca tgcgggtcgc cgtcatcggc ggcgggccgg gcgggctgta
     2641 cgccgcggtg ctgctcaagc gcctcgaccc cgcccgcgag atcaccgtat gggagcgcaa
     2701 cgcacccgag gacaccttcg ggttcggcgt cgtgctctcc gacgagaccc tcggcggtat
     2761 cgagcacgcc gacccggagg tctaccgcgc cctccaggcg gagttcgtcc gctgggacga
     2821 catcgacatc gtccaccggg gacgcaccct gacctccggc ggacacggct tcgcggcgct
     2881 cggccggcgc cggctgctgg ccgtactcca ccggcgctgc cgcgacctcg gtatcgagct
     2941 gcgcttccgc accgaggccc cgcccgccgc cgaactggcc ggtgcgtacg acctggtgat
     3001 cgccgccgac ggagtgcaca gcgccacccg caccgcgcac gccgacgcct tccgcccgcg
     3061 gctggccacc caccactgcc gctacatctg gctggccgcc gacttcgcct tcgacgcctt
     3121 ccgcttcgag atcgcggaga ccgagcacgg cgtcgtccag ctccacgcct accccttcgc
     3181 ccggccggcg ggcccggcgg ccggcgccag cacggtcatc gtcgagatgc gcgaggaggt
     3241 ctggcggtcg gccgggctcg accgctgcga cgagcgcgag tcggccgagt ggtgcgccaa
     3301 ggtcttcacc gacgccctgc gcggccggcc gctgcacggc aacaactcca gctggaccgc
     3361 cttccgcacg gtcgtcaacg accgctggtc gcacggcaac accgtgctgc tcggcgacgc
     3421 cgcgcacacc gcgcacttct ccatcggctc cggcaccaag ctcgccgtcg aggacgccct
     3481 ggcgctggcg gcctgtcttg aggagcagcc cgacacggcc cgcgcgctgg ccgcgtacga
     3541 ggaggagcgc cgccccgtcg tcgcctccac ccagcgtgcg gcccgcgcca gcctcgcctg
     3601 gttcgaggag ctggccacct atctcgacca gccgccgcac cagttcgcct tcaacctcct
     3661 cacccgcagc cgccgcgtca cccatgacaa tctgcggctg cgcgaccccg gattcgtcgc
     3721 ggctgtccag gacgaggcgg gcaccccgcc cggcacaccc ccgatgttcc tgccgttccg
     3781 gctcggtgag ctgaccctgc gcaaccgcgt tgtggtctcc ccgatggaca tgtactccgc
     3841 cgcggaccac aacggcaccc ccggcgactt ccacctcgtc cacctaggcg cccgcgcact
     3901 gggcggggcc ggactggtga tgacggagat ggtgtgcgtc agccccgagg ggcggatcac
     3961 ccccggctgt acggggctgt acgcgccgga gcacgagacg gcctggcgcc gggtcaccga
     4021 cttcgtgcac acctccgccc ccggcaccgc catcggcgtc cagctcggcc actccggccg
     4081 caagggctcc acccggctga tgtgggacgg catcgaccag ccgctcccgg acggcaactg
     4141 gcccctcgtg gcgccctccg cactgcccta ccgccccggc gtcaaccaga ccccgcacgc
     4201 cctgaccgag cccgaactcg ccaccctccg tgagcagttc gtccgtgccg ccgagtccgc
     4261 cgcccgggcc ggcttcgacc tgctcgaact ccactgcgcc cacggctatc tgctctccgg
     4321 cttcctctcc ccgctcacca accaccgcac cgacgcctac ggcggcgatc tcaccggccg
     4381 gctgcgcttc ccgctggagg tcttcgacgc ggtccgtgcc gtctggcctg ccgggcgccc
     4441 gatgacggtc cggatctccg cgaccgactg ggcggacggg ggtaccaccg cggcggacgc
     4501 ggtggcgatg gcgcaggcct tcgccgccca cggcgccgac gccatcgatg tgtccaccgg
     4561 gcaggtggtg tccgacgagc gccctgactt cggccgctcc taccagaccc cgttcgccga
     4621 ccgcatccgc aacaccctcg gcgtgccggt catcgcggtc ggcgccattt cctcctggga
     4681 cgacgtgaac tccctggtgc tggccgggcg tacggacctg tgcgcgctgg ccagaccgca
     4741 tctgtacgac ccgcactgga ccctgcacgc cgccgccgag cagggctacg cgggtcctgg
     4801 cgcgccctgg ccgctgccct accaggcggg cagccgtact ccgcccaccg gacgcctgga
     4861 cgcgccgaag ccccgcctca cgctgcggtg acggggaggc acgggcagcg gggcggggcc
     4921 gatggtgggg ccggtccgga aggacgtcgg cggggccggg cggaccggcg gcgaaggccg
     4981 tcagcggacc ggcgggcagg tcagggcgtc ggtacgcagc tgacctgcgc cgagacgacc
     5041 ggctggccgt gctgttcgac ggcgacctcg acctggtggc ggtcgtcgtc gccccggccg
     5101 gtggtccgcg cctgtaccca ggtcggcgcg tcgagttcgg cgtagcgcga gaaggagcac
     5161 tccatcgaga ccggcagcgt gacgccgggc gtggcggcct gcgcggcctg ccgggcggcc
     5221 tcgaccatca gcatgccggg ggcgtggtcc acggggtggt cgaagagaac cgggtgcgag
     5281 gtgtccaccc gcagttgcca gcggtcgggg cggtcggtgg gggagagcac cacatcgcgg
     5341 tcgtgttccc gggcgaccag ctgcgggcgc acggcctcgg gcagcggcag cgtccgggag
     5401 ttggcgagcg ccagatcgct gtactcgccg cgcagccgtc ggtagacggc cgggctgtga
     5461 caggagtagt cgagcgtcgc agaggccagc tcggcgccgt cgcaccggat ctggaactcc
     5521 tggagggcgc tgaccaggcg cttgccacgg tggaccatgt cgcggtcggt gatgtgcagt
     5581 tcgacctcgg cggggtgctg cgcggtgcgc agcgcctgcg gcgcgaggtc gtagcggacc
     5641 cgctgccaga tgatcgggtg gtcgagcggc acctggtgcg cgacatggct cagtaatatc
     5701 ccggcctgcc gcaccgtctc tatcagcagg atcgggtcgt gcatcccgtg gagcggcccg
     5761 tagaaggtgt gggcgcgcgg ccactgggcg ctgaccaccc agctgtccgc gccgccccgc
     5821 cgccagttcg tcaggaacac ctcggacaac gcgttgcggt ggacatactc acgcgggacg
     5881 gtcgtggtga gcgacgacgg tcggattccg tcggcggatc cgcctccgac ttcgtggcgc
     5941 tgatcgcgca agtacggcgt gagcgtggcg ctggccatag gtggttcctt tctgtggtgc
     6001 ggctctcagc gccctgaagg gctgaggctt ggtcatggcc cgaacacact aagatacgga
     6061 ccatgtggtt tgtttttcgg gcggagccgt cggctccgcc cgagcggtgg cccggggcgt
     6121 cagaccggga tcgtgacgca gcggccgtgc ggatccagat ggctcagcgc accgggcgac
     6181 gcgagacccg gaagcgtgtg cttccacagg gccgtgaccc gctccgggag atcggcgcgg
     6241 ttggtcgcgg cgcgcgagaa caactgcacg cccatgtagg cggcgacgaa gaactcggcc
     6301 gcccggtccg gtgccacgcc gggcagtaat tccccgctgt cccgggcctc ggtgaacatc
     6361 gtggtgatga tgtccgtcca cgcctggtac ggcaccagcg ggtcctcgct gaaggtcgtc
     6421 tccaccgcga tccgcgtgcc ggcctggagc aacgggtcgc tccgcagggc cagtgccacc
     6481 tggtgggtga agtcgatggc gtcctggagc cgggactcac tgacttgtgg cagaaacgga
     6541 tcggtctgct ccgttatgac cgcgcgggcc agtgcgtcct tggacgtgaa gtggaagtac
     6601 accgctccct tggtgacccc ggcgcgctgg atgatctcgg ccatcgtggc ggcctggtac
     6661 ccgcgggtgc cgatgacatg agccgctgct tcgaggacgg cgcgtcgggt gcgcactgcg
     6721 cgttcctgct tgaccaccga tcatcctccg tatgctctct tggagttgtt gtgcctgagt
     6781 gcgacccgct gcccgtggcc gcgcagtcta tttccgcatc ataaagaagc caaccatgcg
     6841 gtattgaatt tgcgcccagt cctaaggcgg gcctctctgg cgtgaaccgg tgtcgtcctc
     6901 aggggcggca ccatcgagtg ccacgagcgt gaggagctgt ccgatgtccc ccaccgtcgt
     6961 ccccgccata cccgccgtgc accgcgaggt ccggctggcg acccaggtcg aaggcgagct
     7021 gacggcacgc cacttcacga tcgccgaggt cccggtgccc gagccgcgcg cgggggaggt
     7081 gctggtgcgc aaccgcgtga tggcggtgac cgcggtgatg cggtcacaga tgaccggaat
     7141 ccggctgccg atgccctcat tcgtaccggg caaggcgctg tggggcgcgg cggtcggcga
     7201 ggtggtggcg gcgcccggag gcggcttcac ccccggcgaa ctggtccatc acccctgcgg
     7261 ctggcgagag ttcgcggtgg tcgacgcgtc gcgcgtacgg agcctcggct cggacgcgct
     7321 gccgaccgcc gccgcgcatc tgtcgcaggg gcgaccgcct ggggtgcctt gcgccgggcc
     7381 gccgaggtca ggccgggaga caccgtgttc atcagtggtg cggcgggcgg cgtgggcagc
     7441 atggccgggc agctcgcacg gcggctgggc gccgggcggg tcgtcggcag caccggctcg
     7501 gagcgcaaag ccgcgcggct gcgcgccgag ttgggctacg acgacacggt cgtgcgcggc
     7561 gccgggccga tcgagcggca gctgcggcgg gccgcgccgg gcggtatcga tgtgctgctg
     7621 gacaccgtgg gcggcgaaca gctgacggcg gcgctcgcgg tggcccgtcc cgaggcgcgg
     7681 ttcgtgctgg tcggcgcact ctccagccag ttgggcgggg accgtggcga catcgccccg
     7741 gtggagctgg acccggggct gatcatcagc cggcgggtgg ccctgcgcgg attcgggctg
     7801 tatgcccatc cggacctgcc gcaggagtgg acgaaggagt tcggccaggg cctgcgggag
     7861 ggcacgctgg tcttcccgca tgcccttctg aagggggtcg aacaggcgcc gcaggcgctg
     7921 cgcgaactga cgcagggtcg tcatatcggg gccgtgctgg tcgagttgtg aggccggggc
     7981 ataccgaacg gggcgggccg gggtcagccg gccgccggaa gctgcgccgg agccgggacg
     8041 gcggccggga tccggcccgg tgcggccggc agcagcgccc acaggtcgac gatgccctcg
     8101 tggtcccacc acgtcccgtc ctcactgccc agatgcagca gaccgaccac gagcgaggtc
     8161 aacagctggg ccgtacgccg cggttcggtg tccgtcgcgt gcggcccggc cggcccgccg
     8221 gtcccacagg tcaccgcttt gtcgagaaac agcgcatacc agcgctgctc cagggcgtag
     8281 ataccgagcc ccggcgccgt ctccggccgc agctgcaagc cggcgcggag caccgcgtcc
     8341 gactccaccc ggctgaacag cgcggtggtg aagtcccgta cggcgatggt cagtggctgc
     8401 gtcgagcggg tgaaggtgtc ctccagctcc tgtagggcct ccagtgccgc gtcccgcacc
     8461 gcgagcgtga ggtcgtcctt cgacgtgaag tggaagtaga gcgctccctt gctcagccgg
     8521 gcccgccggc tgatctcgac catgcccgct gcgggaaccc ccttctccag gaaggtctct
     8581 gcggccgagc ggatgagtgc ctggcggctc tggatggcgc gatgctgggt gggcacggtg
     8641 tccgtcccgt ctctagtacg gctgcgagct cattgcagtc tacatagaaa accaaccatg
     8701 cggttcgata tgcttcacgg gattggccgg aactagcggg ccctgacgcg aaggtgacga
     8761 tctttcgctc gatccggcgc tcggcgcctc ccggggggca tgcgccccca gtctggctgc
     8821 gcaaagaggt gttgtcgacc cgtcagggga cacggggatg cattgacaaa cagcgcaggc
     8881 ggtttttaaa tctccgaagg cggtaaacgg gacgttccct tccgatgcgc cgcctcgacg
     8941 gttgcgtgcc gccccggacg gcggtgcgcc atggcgagac cgccgctgcc gaaaccagtc
     9001 gtcgaccgca ggccgcccca tcagccgagc cagcaacgtc acccgagccc ctgcaccccc
     9061 catcgcaatc cggaaaggtc tccagccatg cgcgtagcaa cggaagtcgg cgaacgtccc
     9121 gtaagaggtg tacggggcgc cggaggtgag acgatccggg ccgccgtctt cgacctggac
     9181 ggcaccctcg ccaacactct ccccgcgatc agcaaactgc tgatcaaggt ggcctcggag
     9241 cagggatgtt cggtgaccgc acggcaggcg gccgccgcga tcggcaagcc gcccggcccc
     9301 gccttcggcc ggctgctcgg caggcccgag gacgacagcc gggtgcaggc cgcgatctcc
     9361 cgctaccgcg aactgttcgc ctacgaagtg ctgtgcctgg ggccgcagct gctcttcccg
     9421 ggcgtgaccg cggggctgtc cgcgctgcgc gcccacgggg tcgagctggc ggtcgccacg
     9481 tccaagacca cccgcagcgc ggaggcgctg ctcgacgtca tgggcatccg cgacctggtc
     9541 ggtccggtca tcggccagga catggtccgg cgcggcaaac cgcacccgga gatggtgctg
     9601 cgggcggccg gccggctcgg tgtcgcggcc tggcagagcg cgtatgtggg ggacaccgtg
     9661 ggggacatga ggatggcggt caccgcgcgc atggagccgg tcgcggtgac ctacggggtc
     9721 ggcacgttcg gcgagctcgc cggggtcgcc ggcacccgga tgtgcggctc cttcaaggac
     9781 gtggtctccg tgatcgccgc cgcccggccg cgctcgggcg tcaaggtggg ggcccagcag
     9841 cgccgccgct gaggccggtg cgcgggggct gctacgcccc ggccatcgcc tcgtgcgcgt
     9901 agggggcccc ggcgtcccgg agtttggcgt gcagccggcc gaacacgtcc gccgcgcggc
     9961 cgcccggcca gtcctcaggc aggaggggag agggcagtcc gggatccgcg tacggcagcc
    10021 ggcgccaggc gtccagcgcc agcagatagt cgcggtaggc cgcttcgggg ggcaccgagc
    10081 ggcgccgcga ccagcgctgg agcaccggct cctgcgcgtc gaggaaggcg cggtgctgtg
    10141 cggcgatggc gtccaggtcc caccaccggg cgacggcgtc cgccgtgggg gcgaagccgg
    10201 cgtgcgtacc ggtgaacagg tcgacgtatg ggtccagttg cagccgctcc agggtgtgcc
    10261 gggtctcctc gtagagctgt gcgggggcga tccacacgcc cggcgcggcg gtgccgaagc
    10321 cgagccggcc cagtcgggag cgcagcagat ggcgcttgtg ccgctcctcc tcggggacgg
    10381 agaagacggc cagcacccag ccgtcggaca gccgtgcggc cggccggccg aagatccggc
    10441 ggtcgccgtc gtccagcagc tggcgggccg cgtcggacag cccgtacgcg gccgcgccgg
    10501 tggccgtccg ggcgggggtc agcagctcgc gccgcttgag ccgggagacc gcggagcgca
    10561 ccgacggcgg atcgaccccg agcgcaccga gcagccggat cagggcggcc accggtaccg
    10621 gggcggccgg gccgcccggg gcgccgggct cgccgcgccc gtaggcgccg tagaacgtga
    10681 cgatcaggga ccgtggggtg cgctgtgtgt gcgggtcgtt caccgggtca ctctagatcg
    10741 ggttcccgca gccggaaacg ctggagcttg ccggtcgggg tgcgcggcag cgaggtgtgg
    10801 aagacgatcg cgcgcgggca tttgtagggc gcgatacggg tcttggtgaa ggcacgcagc
    10861 gcggcgacgg tctcgtcacc ccgggggaca ccgtcccgca gcacgacatg ggcgatcacc
    10921 acctgtccgc ggtcctcgtc cgggcggccg accaccgccg cctcggtcac atcggggt
//
