LOCUS       NZ_CP050976            10921 bp    DNA     linear   CON 06-JUN-2024
DEFINITION  Streptomyces sp. RPA4-5 chromosome.
ACCESSION   NZ_CP050976
VERSION     NZ_CP050976.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RPA4-5
  ORGANISM  Streptomyces sp. RPA4-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9051652)
  AUTHORS   Nicault,M., Bontemps,C., Tidjani,R., Gelhaye,E. and Leblond,P.
  TITLE     Mining the biosynthetic potential for specialized metabolism of a
            Streptomyces soil community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051652)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72x/76x
            Sequencing Technology :: Oxford Nanopore GridION/Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012273495.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 02:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,979
            CDSs (total)                      :: 7,887
            Genes (coding)                    :: 7,432
            CDSs (with protein)               :: 7,432
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 455
            CDSs (without protein)            :: 455
            Pseudo Genes (ambiguous residues) :: 0 of 455
            Pseudo Genes (frameshifted)       :: 276 of 455
            Pseudo Genes (incomplete)         :: 232 of 455
            Pseudo Genes (internal stop)      :: 23 of 455
            Pseudo Genes (multiple problems)  :: 71 of 455
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:36:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 585543
            Orig. end                         :: 596464
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10921
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10921
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10921
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5921](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5921
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    1470..1490
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.12"
                     /tool="antismash"
     gene            <1922..>2239
                     /locus_tag="HEP86_RS40230"
                     /pseudo=""
     CDS             1922..>2239
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /locus_tag="HEP86_RS40230"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="Ku protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPNGRTVVLDDSDLADLPLPGVKAIEVLGFLPSDQIAPNALDRSY
                     FLGVEGAGARPYRLLRDAMKQSGQVGVARMALRGPERLVVLRVREEVIVAQKLLWPDEV
                     RS"
     gene            complement(2594..3034)
                     /locus_tag="HEP86_RS40235"
     CDS             complement(2594..3034)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /locus_tag="HEP86_RS40235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_248000973.1"
                     /transl_table=11
                     /translation="MDRGKPGSKMHVLSDADGLPLRVGVSAANTHDSLALKPMLSHFHM
                     GHELHAADSKPGRLHADKAYDVPHLRRWLWGKHMGARIARKGIESSERLGRRRWVIERT
                     HVLADRLPPPQHRYERKPGNYLAFLGLAAALCCYKRLLKLTM"
     gene            complement(2926..3393)
                     /locus_tag="HEP86_RS40240"
     CDS             complement(2926..3393)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /locus_tag="HEP86_RS40240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_248000974.1"
                     /transl_table=11
                     /translation="MGPIVPDGLWEIARPLLPPARVRPQGGGVANIDDEAVFAAIIYVL
                     VSGCAWRALPPCFGASKSTVHRRFVIWSRAGVSGRLHQEVLQILDEQGLVDLSRAVLDS
                     AHLRAKKGANSQVRAPWTGASRVPRCTSCPMRTDCPFASASPRPIPTTASR"
     misc_feature    3683..3701
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            3684..4067
                     /locus_tag="HEP86_RS40245"
     CDS             3684..4067
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEP86_RS40245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_248000975.1"
                     /transl_table=11
                     /translation="MRTGRRSARPRSSSAVGNPEHDWFYLEEMQAWEKSGIAEVHTAFS
                     AVPDHPARFVQHAIAAASDSVWNALEDGAYLYVCGDGRFMAPAVREALAALHRARTGSD
                     DDASQQWLAQLEADGRYQQDVFA"
     gene            complement(4282..4914)
                     /locus_tag="HEP86_RS02840"
                     /old_locus_tag="HEP86_02840"
     CDS             complement(4282..4914)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006601757.1"
                     /locus_tag="HEP86_RS02840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02840"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_168476989.1"
                     /transl_table=11
                     /translation="MTFEVYVQIRAETTRRFLLEAAATLFDEKGYAGTSISDISAHSGR
                     TSGAIYFHYASKEKLALAIVEEHFATWPHLISRYRQPGRPALEKLVGLSFEVARAFRDD
                     IVVRAGARLWAERKAIDAQLPAPFLGWIEAVTQLLEEAARNGEVAADVPPAATAHGIVC
                     SFFGLHTVSDALEGRRHIEERLHDLWTLLLRGLQAEPDPAGLLDRVR"
     gene            5001..5921
                     /locus_tag="HEP86_RS02845"
                     /old_locus_tag="HEP86_02845"
     CDS             5001..5921
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="HEP86_RS02845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02845"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_168476991.1"
                     /sec_met_domain="AfsA (E-value: 2.6e-14, bitscore: 51.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTVRSVEKSEPLSWSRTVERELVHRNSVAEVLLTEVRPTDTP
                     HIFLAAASWPRAHATFPRDGSQRHSPLILVETLRQLGLYIPLRFYAVPEAFRAVITDLY
                     FHLSPADELPARAGATEITCSARVSAVRRLPDASAVGLRLNVTFAACGVTFGQGGGGVR
                     FLEGARYTALRARSAVDAVPARRPEGGYRPPTAQLTVNHPHDAVLTLDQGELLVSPADP
                     LHPFFFDHPTDHVPGMVLLEAARQAASYRSRGRLSRPLFGRLKASWFTELTPFARVLCT
                     RHGSMCSFRILQDGTETACGTFSYT"
     misc_feature    5981..5996
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            6144..6863
                     /locus_tag="HEP86_RS02850"
                     /old_locus_tag="HEP86_02850"
     CDS             6144..6863
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151397.1"
                     /locus_tag="HEP86_RS02850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02850"
                     /product="HAD family phosphatase"
                     /protein_id="WP_168476993.1"
                     /sec_met_domain="HAD_2 (E-value: 2.9e-26, bitscore: 91.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADSILSWTPAAVVFDCDGTLMDTERHWEDARNMVLHSYDCAPS
                     EGFAEKARGLHYTECGRLMADVAGRPELTGDMTESLLKHFRELVAADPTTMPGAPELVH
                     SAVQFAPLAVASNCPLEVVESCLGMAGLRDCFSHIVVPNDTTRPKPHPDVYLTAAQLCG
                     VEPADTLAVEDSHCGIQAAAGAGMRVLGVGPRPSDRETALADWWVHSLAEPAVQGWADA
                     RIPAQGTRPTGPGTTTA"
     misc_feature    6330..6332
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7251..8066)
                     /locus_tag="HEP86_RS02855"
                     /old_locus_tag="HEP86_02855"
     CDS             complement(7251..8066)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (4-161). E-value: 3.6e-16. Score:
                     51.5. Matches aSDomain:
                     nrpspksdomains_HEP86_RS02855_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576057.1"
                     /locus_tag="HEP86_RS02855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02855"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_168476995.1"
                     /sec_met_domain="adh_short (E-value: 4.3e-31, bitscore:
                     107.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.1e-34, bitscore:
                     118.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQGVVLVTGGGTGIGAATARLLRTAGHQVVISGRRPEPLRRVA
                     EETGALAHPSDAADPEAVRELVEATVTAYGRLDGVVLNAGIGRGGAVGDTAVEDWEELI
                     RTNLTGPFLLLRAALPHLLAARGTVVAVASVGALRNSVGNATYATSKAGLLHLCRSLAV
                     DYGPQGLRANAVCPGWVRTEMADQRMARFAAEAGLAGGAEEAYEEANRLNPARRPGDPE
                     EVAEAIDWLLSPASSYVNGAVLTVDGGVTQVGVGGAAFDYRIEARTPRP"
     aSDomain        complement(7584..8054)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEP86_RS02855_PKS_KR.1"
                     /evalue="3.60E-16"
                     /label="HEP86_RS02855_PKS_KR.1"
                     /locus_tag="HEP86_RS02855"
                     /protein_end="161"
                     /protein_start="4"
                     /score="51.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="GVVLVTGGGTGIGAATARLLRTAGHQVVISGRRPEPLRRVAEETG
                     ALAHPSDAADPEAVRELVEATVTAYGRLDGVVLNAGIGRGGAVGDTAVEDWEELIRTNL
                     TGPFLLLRAALPHLLAARGTVVAVASVGALRNSVGNATYATSKAGLLHLCRSL"
     CDS_motif       complement(7956..8051)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEP86_RS02855_0001"
                     /evalue="4.60E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="HEP86_RS02855"
                     /protein_end="37"
                     /protein_start="5"
                     /score="9.7"
                     /tool="antismash"
                     /translation="VVLVTGGGTGIGAATARLLRTAGHQVVISGRR"
     misc_feature    8309..8323
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(8696..8710)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.56"
                     /tool="antismash"
     gene            complement(8765..>9038)
                     /locus_tag="HEP86_RS02860"
                     /old_locus_tag="HEP86_02860"
                     /pseudo=""
     CDS             complement(8765..>9038)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982115.1"
                     /locus_tag="HEP86_RS02860"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="HEP86_02860"
                     /product="NAD(P)-dependent oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PTSLAPLLADWLVAMARRFIRPPTSCRVTTSPRVSSPVSPCRWPA
                     RQPF"
     gene            complement(<9246..9377)
                     /locus_tag="HEP86_RS02865"
                     /old_locus_tag="HEP86_02865"
                     /pseudo=""
     CDS             complement(<9246..9377)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141279.1"
                     /locus_tag="HEP86_RS02865"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02865"
                     /product="flavin reductase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRTEFDPAAMSRNEFYRLLTATVVPRPIAWVSTTSADGTHNLAP"
     misc_feature    complement(9344..9354)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    9375..9390
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.23"
                     /tool="antismash"
     misc_feature    complement(9842..9858)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.83"
                     /tool="antismash"
     gene            9853..10515
                     /locus_tag="HEP86_RS02870"
                     /old_locus_tag="HEP86_02870"
     CDS             9853..10515
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981564.1"
                     /locus_tag="HEP86_RS02870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEP86_02870"
                     /product="response regulator transcription factor"
                     /protein_id="WP_168476997.1"
                     /transl_table=11
                     /translation="MRPTILVVEDDHALRDVLVRGLRDEGFATLAAQDGATALRLADDG
                     MDAVVLDVGLPDADGRDVCQAMRARGVVSPVIFLTAHHQLHDRLSGFSAGGDDYLPKPF
                     HLTELAARLRAALKRCGPPAPTATGDLVLDPVHHSLGVRGVDVALTPTEFRLLAALMAG
                     LGAVVPRKGLVRAGWPEGAHVSDNTLDQYLSRLRRKIREAGSDLTISTVRGVGHRLS"
     misc_feature    10624..10638
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
ORIGIN
        1 gacggtgatc agcagcacca cgaacaccgt cctggccacc gtccccgccg gctcaggccc
       61 ggccatgatc gcggtggcgc cgaacggcaa cgtctacgtc accaaccaga gctcgaacaa
      121 cgtgacggtg atcagcagcg ccaccaacac cgtcctggcc accgtccccg tcggcggggg
      181 cccgttcgca ctggcggtgg cgccgaacgg caacgtctac gtcgccaaca gcacttcgaa
      241 caacgtgacg gtgatcagca gcgccacgaa cacggtcctg gccaccgtcc ctgtcggcgc
      301 ggtcccggac gcggtggcgg tggcggcgaa cggcaacgtc tacgtcgcca accggaacgc
      361 gaacaccgtg acggtgatca gcagcgccac gaacaccgtc ctgaccaccc tccccaccgg
      421 tggtttccca ggcgcggcgg cggtggcgca gaacggcaac gtctacatcg ccaaccagac
      481 ctcgaacaac gtgacggtca tcgacagcgc cacgaacacc gtcctggcca ccgtccccgc
      541 cggcacggtc ccgggagtga ttgcggtggc gcccaacggc aacgtctacg tcaccaacac
      601 cgtttcgaac aacgtgacgg tgatcgacag cgccacgaac acggtcctgg ccaccgtccc
      661 caccggcggg ggcccgttcg gggtggcggt ggcgccgacc ggcaacgtct acgtcggcaa
      721 cagcagttcc aacaatgtga cggtgatcag cagcgccacg aacacggtcc tggccaccgt
      781 ccccgtcggc gcgttcccct tctcggtggc ggcggcgccg aacagcaacg tctacgtcac
      841 caacgccaat tcggccaacg tgacggagat ctcgccgttg acggtgacga cctcgcctgc
      901 ggcgccggtg tgcgggcaac cggtgacgtt cagcatttcc ggggggactc cgaacggcac
      961 agcggtggtg aacttcgggg acagcagccc cacggtcacc gtccccctgg acgccaccgg
     1021 gagcgggcag accacccaca cctacaccgc cggtacgttc acagcgaccg tgaacggcaa
     1081 ccccaccccc gtcaccgtga actccaaccc cacaacgacg acgctgtcgg tgacgccgaa
     1141 cccctccacc tgcggtcaga acgtgacggt atgcgccacc atcaccccgg caccagccac
     1201 caccacagtg ccgaccggca cggtcacctt caccctcccc aacggccaaa cccaggtggt
     1261 ccccgtcaac gcatcggggc aggcctgctt caccaccacc gccctgacca ccggcaccct
     1321 caccgccgcc tacggcggtg acagctgctt caccggctcc aacgccagca cccccgtcac
     1381 cgtgaaccgc acccccacaa agctgaccgc gcagccaggc acgatccggc tccgtctgac
     1441 cccgctgccc gagttctaca tccccaccct cagcgccacc ctgacgacca cctcggggat
     1501 gccggtggcc ggtcagccgg tgaccttcac cgccaccacg ctcttcggcc cggtcaacct
     1561 cggcaccgcc gtcaccgacg ccaacggcac cgccaccatc cacaacgcca tcgtccccgt
     1621 cttcgccatc gccaccccct tctacaccgc caccttcccc ggcacttcct gctacaccgc
     1681 cgcaaccgac cacaacatcc tgctgttcat ccccctcccc agctgacctc gtcggctccg
     1741 aaaacccacg tcacctgatc tgctgcgacg ggaggaacac ttcgtgaccc agctgcacga
     1801 gttggccgcc cagacccggc cttcctcccg tgcatgccgt cgagggggcc agaatccggc
     1861 aacggcgcat ctgcgaggct gaaggcgccg aggtgccgta ccagggggtc gcggctacga
     1921 ggctccgaat ggccgcaccg tcgtgctgga cgacagcgat ctcgcagatc tgccccttcc
     1981 cggcgtgaaa gcgatcgagg tgctcggctt cctgccgtca gaccagatcg cccccaacgc
     2041 attggacagg tcgtacttcc tgggcgtcga gggagccggt gcccgcccgt accggttgct
     2101 gcgggacgcg atgaagcaga gcggccaggt cggagttgcc cgcatggcgc tgcgcggtcc
     2161 ggagcgtctc gtggtcctcc gggtccgcga ggaggtgatt gtcgcgcaga agctgttgtg
     2221 gccagacgag gttcgctccg ctgactcctt ggtggcctct gccccggagc cgcgcgtggt
     2281 gacggcgaag ctcgccgggc aggagccacc gcatgcaccc gtcgtgttgc gccggtccga
     2341 ccgtacgcag caggccccgg aactgagttc cgggggcctg ctgctcagga cgatggttcg
     2401 ggggaggttc agcgtgtgcg actcgcagtg tggggtgctg gtggtgccgg ggccttctgg
     2461 gcggttcgac cagagactcg aaacggtgtg ggagtcttcc aggcgtctgg gaaccggacc
     2521 cccgtgtact gctcgatggc atcgcgaaga ccggcaggaa ctgcgccttt ccaaggcggc
     2581 ctaagaccgt gtcctacatg gtcagtttga ggagtcgttt gtagcagcag agggcggctg
     2641 ccagtccgag aaaggccagg tagttgcccg gttttcgctc gtagcggtgt tgaggcggcg
     2701 gtagccggtc agccaggaca tgggtgcgtt cgatgaccca gcggcggcgg ccgaggcgtt
     2761 cgctggactc gatgcccttg cgggcgatgc gggcgcccat gtgtttgccc cagagccatc
     2821 gccgcaggtg ggggacgtcg taggccttgt cggcgtgaag gcgcccgggc ttggagtcgg
     2881 ccgcgtgcaa ttcgtgtccc atgtggaaat gggacagcat cggcttcagc gcgaggctgt
     2941 cgtgggtatt ggccgcggag acgccgacgc gaaggggcag tccgtccgca tcggacagga
     3001 cgtgcatctt ggaacccggc ttgccccggt ccacggggct cggacctgtg agttcgcccc
     3061 ctttttagcg cgtaggtggg cggaatcgag gactgctcgg gacagatcca ccagcccctg
     3121 ttcgtccagg atctggagga cttcctgatg cagccgtccc gagactccgg cgcgagacca
     3181 gatgacgaac cggcggtgca ccgtggattt cgatgctcca aagcagggcg gcaacgctcg
     3241 ccatgcgcag ccgctgacca agacgtagat gatcgcggcg aagactgcct catcatcgat
     3301 gttcgcgacc ccaccgccct gcgggcgcac ccgcgccggc ggcagcaacg gcctcgctat
     3361 ctcccacagg ccgtccggaa caatggggcc catccgcccc cgcttctact ccatctcgtc
     3421 ctcgtccctg gccgacccgc ggcatgtccg actgaccgtt ggactcgtcg aaggtcccgc
     3481 gctgtcgggt gggggcgagt accggggcct gtgcccgcag tacttgggcc ggctggagcc
     3541 cggagatgtc ttctacggct atgtgcgcgt gccgtcaccg acgttcgcgc cgccggcaga
     3601 tcccgcgacg cctctcctcc tcatcggccc cggtaccggc atcgcgccgc tgcgcggctt
     3661 cctggaggaa cgcgcctggc agcatgcgaa cgggacgcag gtcggcacgt cccaggtctt
     3721 cgtcggctgt cggcaacccg gagcacgact ggttctacct ggaggagatg caggcctggg
     3781 agaagtccgg gatcgccgag gtgcacactg ccttctccgc agtccccgac caccctgccc
     3841 ggttcgtcca gcacgcgatc gccgccgcct ccgattcggt gtggaacgct ctcgaagacg
     3901 gcgcgtacct ctacgtctgc ggagacggcc gcttcatggc accggccgtg cgtgaggcac
     3961 tcgccgccct ccaccgcgcc cgtaccggaa gcgacgacga cgcctcccag cagtggctcg
     4021 cccaactcga ggcggacggg cgctaccagc aggacgtctt cgcctaatgg gagctgccgg
     4081 gtcgtagaga ctcggtgccg tagccgccag caggtgagca cttcatcgcc tgaagcacgt
     4141 ggcttctgag gtcagaccgt gcccctgctg gtcggccggg aggccagacc gttgatgggg
     4201 cggcgtgccc gccggaccag gcgcagctgc agactgcggc cttcctcagc gtctgcgaga
     4261 tcggtgggcg tcgcgcgccg ctcagcgcac gcgatcgagc agaccggcag gatcaggctc
     4321 ggcctggagg ccgcggagca gcagagtcca caggtcatgc aggcgctcct cgatgtgccg
     4381 gcgtccctcc agggcatcgg agacggtgtg cagaccgaag aaggagcaga cgatcccgtg
     4441 ggcggtggcg gcaggtggca catcggcagc gacttcaccg ttacgggcgg cttcttcaag
     4501 tagctgtgta acggcttcga tccagccgag gaacggtgcg gggagctggg cgtcgatggc
     4561 tttgcgttcg gcccaaaggc gggctccggc ccggacgacg atgtcgtctc gaaaggcgcg
     4621 tgccacctcg aagctcagcc cgacgagttt ctccaaggcc ggccggccgg gctgccggta
     4681 gcggctgatc aaatgcggcc acgtggcgaa atgctcctcg acgatggcga gggcgagctt
     4741 ctccttgctg gcgtagtgga agtagatggc cccactggtg cgaccggaat gcgcactgat
     4801 gtcgctgatg ctggtaccgg cgtacccttt ctcgtcgaac aaggttgccg ccgcctcaag
     4861 aaggaaccta cgcgtcgttt ccgcccggat ctgcacgtac acctcaaacg tcatctcgtt
     4921 ccgccggaag gcaacctacc gctccccgcg ccccggaatt caagggtgcg catgttcgaa
     4981 tttcggtatt ccctggtcgt atgagcacgt caacggtcag gtccgtcgaa aagagtgagc
     5041 cgctcagctg gtcccgtacc gtcgagcggg agctggtgca ccggaactcg gtggcagagg
     5101 tcctccttac ggaggtacgg ccgaccgaca ccccgcacat cttcctggcg gcggcgtcat
     5161 ggccacgcgc gcatgcgacg ttcccccgcg acgggtcgca gcggcacagc ccgctgattc
     5221 tggtggaaac actgcgtcag ctcggtctct acattcccct gcggttctac gcggttccgg
     5281 aggccttccg tgcggtcatc accgacctgt acttccacct cagccccgcc gacgagttgc
     5341 cggcccgcgc cggagcgacc gaaatcacct gctcggcccg ggtcagcgcc gtacggcggc
     5401 tgccggacgc gtccgccgtc ggactgcggc tgaacgtcac cttcgccgcg tgtggggtga
     5461 cttttggcca aggcggcgga ggtgtgcgat tcctggaggg cgcgcgctac accgcgctgc
     5521 gcgccaggag cgcggtcgac gccgtcccgg ctcgccgtcc ggagggcggg taccgcccgc
     5581 cgaccgcaca gctgaccgtg aatcacccgc atgatgcggt cctcactctc gaccaggggg
     5641 agctgttggt ctcgccggcc gacccgttgc acccgttctt cttcgaccac cccaccgatc
     5701 acgtgcctgg catggtgctg ttggaagccg cccgccaggc cgcctcatac cgcagtcggg
     5761 gcagactgag ccgacccctt ttcggccggc tgaaggcctc ctggttcacc gaactgaccc
     5821 cattcgcacg tgtgctgtgc acccggcacg gctcgatgtg ttcattccgc attctccagg
     5881 acggcacaga aacagcctgc ggcaccttca gttatacgta gagcacacaa tattgagatt
     5941 tgcgaattgg ttgacctttc tgcgtcgcca tccgtaacat aacgatcgtt attttccggc
     6001 tggagcggaa gaactcctct ccctgggagc tgccctgccg gaacccctca gcccggctcc
     6061 gggcgtccgt gaaaccgacg ctgccatgct ctctcgcgac cggtcgtatc ggcgccttcg
     6121 ggcccatcag aacggaatcc cgaatgaccg cagacagcat cctttcctgg acccccgcgg
     6181 ccgtcgtttt cgactgtgac ggcacgctga tggacaccga acgccactgg gaggacgccc
     6241 gtaacatggt gctgcacagc tacgactgcg caccctcgga gggtttcgcg gaaaaggcgc
     6301 gagggctgca ctacaccgaa tgcggccgtt taatggccga tgtggccggg cgaccggaac
     6361 tgaccggtga catgacggaa tcgttgctca agcacttccg tgaactcgtc gccgcagacc
     6421 ccacaaccat gcccggggcg ccggaattgg tgcattcggc cgtgcagttc gccccgctgg
     6481 ccgtggccag taactgcccg cttgaagtcg tcgagtcctg tctcggaatg gctggactcc
     6541 gggattgctt cagccacatt gtggtaccca acgacaccac gagacccaaa cctcatccgg
     6601 acgtctacct caccgccgcc caactctgtg gggtggagcc cgctgacacg ctggccgtcg
     6661 aggactccca ctgcggcatc caagctgccg caggcgcggg catgcgggta ctcggagtcg
     6721 gtccccgccc cagcgacagg gagacagcgt tggccgactg gtgggtccac tcgctggcgg
     6781 agccggcggt gcagggatgg gccgacgccc gcatccctgc ccagggcact cgtcccactg
     6841 ggcccgggac cacgacggca tgacggcgac ccggcccgcg gcagactcca tgacggccgc
     6901 gggtccgcgg gccgggtggc atccagggtg acctgcggtg cgggcgccac cacctcgtca
     6961 ggccatgagg tacccggcat ccaacggcgc gcacccgcaa ggtgccccac accgacgcgg
     7021 ctctccccgc agaaggaggt gggcagaggc ctgacatgga gttgacgaac accacccggc
     7081 gctctcctcg caggcggagg cgaacgcgac aaccggtgcc ggctcgccga ccggaacgcg
     7141 caccccagcc ccttcacctg gaacaagacc gccgacgaaa tcctcgacaa gtcgccgcct
     7201 actgtcaccg aacctctgag tcaggtcacc agggcctgtc cgggagtccg ctacgggcgc
     7261 ggggtgcgcg cctcgatccg gtagtcgaag gcggcgccgc cgacgccgac ctgggtcact
     7321 ccgccgtcga cggtgaggac ggccccgttg acgtaggacg aggcgggcga cagcagccag
     7381 tcgatcgcct cggcgacctc ctccggatcg cctggccgcc gggcgggatt cagccggttc
     7441 gcctcctcgt atgcctcctc ggcaccgccc gccagccccg cctccgccgc gaaccgcgcc
     7501 atccgctggt cggccatctc cgtacgcacc cagcccgggc acaccgcgtt ggcccgcagc
     7561 ccctgcggcc cgtagtcgac ggcgagggag cggcagaggt ggagcagacc cgccttggac
     7621 gtcgcatagg tggcgttgcc gacgctgttg cggagcgcgc cgaccgaggc gacagcgacc
     7681 accgtgccgc gggccgccag cagatgcggc agcgcggcac gcagcagcag gaacgggccg
     7741 gtgaggttgg tccgtatcag ctcttcccag tcctcgaccg cggtgtcgcc aactgcgccg
     7801 ccccgcccga ttccggcgtt gagcaccaca ccgtcgagtc tcccgtaggc cgtcaccgtc
     7861 gcctcgacga gctcgcgcac ggcctcgggg tcggcggcgt cggaagggtg ggccagcgct
     7921 ccggtctcct cggccacccg gcgcagtggc tcgggccgcc gcccggagat cacgacctgg
     7981 tgcccggcgg tgcgcagcag ccgggcggtg gcggccccga tccccgttcc cccgcccgtc
     8041 accaggacaa caccctgctc cgacacgctc gttcgccaac ctgtacatgc ccatcgatgc
     8101 gctgttcttc ttcggcgaca gcgccggtgg tggccagttc gcgttcgtga ggacacctcg
     8161 ccgtgccgac atcttcgtgt gggagcacga ggacgacagc cgacgtgggt cgcccatgat
     8221 ctacacgact acctcggccg gacgctcgca gacggcggag acgcttggta ctgacctgaa
     8281 cgcccctgac cgacacccga tagggggtgg tggccgtcgg ccaggccgtg ccctgacgcc
     8341 ctcctccgcg gcgtcggccc cgctcaggtc gccagctggc cacggccgca gagccaacca
     8401 cggaggatct cccagagcta ccgcaagtga tgcggtaagt acgggtgctc ttgctcacgt
     8461 cgtaggcacc gcttactttc gcgagatgac tacggattcg cgagacacgc ccgcttccgg
     8521 cagaggtggc ttgtgttgct gcctgatggt cgcggccctg ccgctcgtct ggttattcgc
     8581 cgctccgctg ctgccggagc aggtgcgttg gctccccttt tggctggggg ccatgatgtg
     8641 gaacggagca gatggccgag gcggcatcag ccacctgttc ggctagggcg tgtggtgaaa
     8701 atgcgtcaca gcctgtggcg gagggctgtg gtagcacggt cacctccgag ggccaagcgg
     8761 ccggtcagtg caccagcagg tcgatcacgc ccgtcaggcc ctcctcgcca ttgtcttcgg
     8821 ccaggcgacg gcgcattagc tcgaagtaga ggctgagcac ttccggactg acaccctgct
     8881 gttggtgctc agaaaggttg gcgtgccggc caccggcatg gcgagactgg agacgacacc
     8941 cttggtgaag tcgtcactct gcagctggtc ggcggtctga tgaaccgacg ggccatcgcg
     9001 acgagccagt cggcgagcag cggggcgagc gacgtggggc gcgggagctc tgcctacctt
     9061 cgacagtctc cgccggggcg taaggccggc aagcacgatc acttcagcac gcgacgtcag
     9121 gtagctcccc cctccgcctc tgccgactgc ttgtccgcgc ccagccattg ggcgtactca
     9181 ttgccgtgag gcgctgacct gggctaatgt cgcgactccc gataccactg tcttcagaac
     9241 ttcgcagggg ccaggttgtg cgttccatcc gccgatgtag tggacaccca ggcgatgggc
     9301 ctcggcacca cggtggcggt cagcagccgg tagaactcgt tgcgggacat ggcagcgggg
     9361 tcgaactcag tgcgcatggt gatcagtatc caggtgtgtg gccgcggacc gtacagggat
     9421 ccacggcgcc accaaacctc tcgccatccg atagacgctc cacacccacg catatcgctg
     9481 aatagctccg cccttgaggc cggcgggggc gaccgtcgag ctgggacact gccgggcggg
     9541 cgacgccctt ctcccccgca gcctgcgccc tcgatcacgc accgcccccg ctcccgctcc
     9601 catcgtctac agtgcagtgg accgccgacc cgcttgtagt cgcatgtggc gtggccaccc
     9661 ggccgtcccc cagcgccccc gcgcaaggac aaccgtccag agacggacag tgcagcggca
     9721 cgagagcgga tgtccactcc gtgcccgaca tgggctctgt ggaccgctgc cgccgcctgt
     9781 ccgcctgcgc cgggggctgc gtctggccct ctgtaccccg gggcgagcgc ccgagccagt
     9841 gaggtgattt tgttgcgtcc caccatcctg gtcgtcgagg acgaccacgc cctgcgggac
     9901 gtgctggtgc gcgggctgcg cgacgaaggc ttcgcaacgc tggccgccca ggacggtgcc
     9961 accgccctgc ggctggctga cgacggcatg gacgcggtgg tgctggacgt cggactgccc
    10021 gacgcggacg gacgggacgt gtgccaagcg atgcgggccc ggggagtcgt cagcccggtc
    10081 atcttcctga ccgcgcacca ccaactccac gaccgcctca gcgggttctc cgcggggggc
    10141 gacgactacc tccccaagcc attccacctc accgagctcg ccgccagatt gcgcgccgcc
    10201 ctcaaacgtt gcggaccccc cgcgcccacg gctaccgggg acctggtcct cgatccggtg
    10261 caccacagcc tgggcgtgcg gggcgtcgac gtggcgctga cccccaccga gttccggctg
    10321 ctggccgcgc tgatggccgg cctcggggcg gtggtgccca gaaaggggct ggtaagggcc
    10381 ggctggcccg agggcgcaca cgtcagcgac aacacgctcg atcagtatct gagccgtctg
    10441 cggcgcaaga tccgggaggc gggcagcgac ctgacgatca gcaccgtccg cggcgtcggg
    10501 caccggctgt catgaccgcc cgaaaccgct ttgtcacgtt ggccgcccgc tgccgcccac
    10561 gcactttgcg gggccggttg tccctggtgg cgctcaccac cgccgcgctg gtgatggtga
    10621 tcctcaccgt cgtgttcaac acgatcgtcc gccagcgcct gcagcagcag gcggacgacg
    10681 agctgcgtac ccgggcctcc gccgtggccg cgaccgtcga cacgcgccac cacccggtac
    10741 gtgtcctgga gaccccggac gacgccgtcc tcgacaccaa cgtatggatc cacgcgggca
    10801 gccggctgct ggagcacccc gcctccacgc cgcccggcga ccccatcagc cgcaccgcgg
    10861 cgaagctcgc cgcgcgcggc ggcagacact gtgccgaggt gacggccggc gacccgatac
    10921 g
//
