LOCUS       NZ_VIOH01000014         9424 bp    DNA     linear   CON 03-JUL-2024
DEFINITION  Bacillus subtilis strain FH1 NODE_16_length_9314_cov_126.016747,
            whole genome shotgun sequence.
ACCESSION   NZ_VIOH01000014
VERSION     NZ_VIOH01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Khan,M.A., Khan,M.N., Imran,M., Hameed,A. and Hasan,F.
  TITLE     Draft genome of Bacillus subtilis FH1 isolated from tannery waste
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Khan,M.A., Khan,M.N., Imran,M., Hameed,A. and Hasan,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2019) Department of Microbiology, Quaid-I-Azam
            University, Facutly of Biological Sciences, Islamabad, Federal
            45320, Pakistan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012028945.1-RS_2024_07_03
            Annotation Date                   :: 07/03/2024 03:57:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,286
            CDSs (total)                      :: 4,159
            Genes (coding)                    :: 4,053
            CDSs (with protein)               :: 4,053
            Genes (RNA)                       :: 127
            rRNAs                             :: 10, 16, 11 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 7, 16, 11 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 62 of 106
            Pseudo Genes (incomplete)         :: 50 of 106
            Pseudo Genes (internal stop)      :: 18 of 106
            Pseudo Genes (multiple problems)  :: 21 of 106
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:36:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9424
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VIOH01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9424
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:7709](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..7709)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3242)
                     /gene="srfAB"
                     /locus_tag="FLN47_RS21480"
                     /pseudo=""
     CDS             complement(<1..3242)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (12-415). E-value: 1.2e-115.
                     Score: 378.3. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21480_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (521-588). E-value: 1.1e-25. Score:
                     81.6. Matches aSDomain: nrpspksdomains_FLN47_RS21480_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (606-891). E-value:
                     1.4e-105. Score: 344.5. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21480_Condensation_LCL.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="FLN47_RS21480"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.3e-126, bitscore:
                     410.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.8e-18, bitscore:
                     54.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 8.8e-100, bitscore:
                     322.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQITEATFAALFEKQAQQTPDHSAVKAGGNLLTYRELDEQANQL
                     AHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSG
                     AKFAVVNERNMTAIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKP
                     KGVQIEHRNLTNYVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQK
                     ETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPT
                     DVLAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLN
                     EALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSD
                     GTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQ
                     AIEKAELRKQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNTAQSGGKALAAP
                     ETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKSVPLKALFE
                     QPTVRQLAAYMEASVVSGGHQVLKPADKQAIYPLSSAQKRMYVLNQLDRQTISYNMPSV
                     LLMEGELDISCLRDSLNQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEA
                     DQKIKEFIRPFDLNDAPLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYK
                     GEQLPQPALHYKDFAVWQHEAEQKERMKEHEAYWLSVLSGELPELDLPLDYARPPVQSF
                     KGNTIRFRTGSETAKAVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRT
                     NADVQDMPGMFVNTLALRMKAKEQQTFAELLEQAKQTNLSALEHQEYPFEDLVNQLDLP
                     RDMSRNPLFNVMVTTENPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQL
                     EYATDLFAKETAEKWSEYVLRLLKAVADNPNQPLSSLSLVTETEKQALLEAWKGKALPV
                     PTDKTVHQLFEETVQRHKDRPAVTYNGQSWTY"
     source          1..9424
                     /altitude="0.3 m"
                     /collected_by="Dr. Abdul Hameed"
                     /collection_date="1992-11-20"
                     /db_xref="taxon:1423"
                     /geo_loc_name="Pakistan: Lahore"
                     /isolation_source="tannery waste"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="FH1"
                     /submitter_seqid="NODE_16_length_9314_cov_126.016747"
     region          1..9424
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9424
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(414..527)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0009"
                     /evalue="6.20E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="943"
                     /protein_start="905"
                     /score="57.2"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(570..1424)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21480_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-105"
                     /label="FLN47_RS21480_Condensation_LCL.1"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="891"
                     /protein_start="606"
                     /score="344.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="IYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISCLRDSL
                     NQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLNDA
                     PLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPQPALHYKDFAV
                     WQHEAEQKERMKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGNTIRFRTGSETAKA
                     VEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTLA
                     LRMK"
     CDS_motif       complement(576..665)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0008"
                     /evalue="1.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="889"
                     /protein_start="859"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       complement(924..956)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0007"
                     /evalue="1.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="773"
                     /protein_start="762"
                     /score="14.5"
                     /tool="antismash"
                     /translation="HYKDFAVWQHE"
     CDS_motif       complement(987..1052)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0006"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="752"
                     /protein_start="730"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1248..1358)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0005"
                     /evalue="7.40E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="665"
                     /protein_start="628"
                     /score="50.5"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISCLRDSLNQLVNRHESLRTSF"
     aSDomain        complement(1479..1679)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21480_PCP.1"
                     /evalue="1.10E-25"
                     /label="FLN47_RS21480_PCP.1"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="588"
                     /protein_start="521"
                     /score="81.6"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KSVPLKALFEQPTVRQLAAYME"
     aSModule        complement(1479..4481)
                     /complete
                     /domains="nrpspksdomains_FLN47_RS21485_Condensation_LCL.1"
                     /domains="nrpspksdomains_FLN47_RS21480_AMP-binding.1"
                     /domains="nrpspksdomains_FLN47_RS21480_PCP.1"
                     /locus_tags="FLN47_RS21480"
                     /locus_tags="FLN47_RS21485"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1968..2033)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0004"
                     /evalue="3.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="425"
                     /protein_start="403"
                     /score="38.1"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(1998..3206)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21480_AMP-binding.1"
                     /evalue="1.20E-115"
                     /label="FLN47_RS21480_AMP-binding.1"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="415"
                     /protein_start="12"
                     /score="378.3"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHSAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKFAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPTDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       complement(2115..2204)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0003"
                     /evalue="4.70E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="376"
                     /protein_start="346"
                     /score="50.9"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       complement(2733..2792)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0002"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="170"
                     /protein_start="150"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(2979..3020)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21480_0001"
                     /evalue="3.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="FLN47_RS21480"
                     /protein_end="88"
                     /protein_start="74"
                     /score="16.9"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     gene            complement(<3315..>4487)
                     /locus_tag="FLN47_RS21485"
                     /pseudo=""
     CDS             complement(<3315..4487)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     7.2e-95. Score: 309.4. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21485_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="FLN47_RS21485"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 2.4e-86, bitscore:
                     277.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEMIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTDYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAAN"
     CDS_motif       complement(3423..3470)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0006"
                     /evalue="1.00E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="355"
                     /protein_start="339"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="HHVAKFDLSLEAVERE"
     CDS_motif       complement(3534..3647)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0005"
                     /evalue="7.60E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="318"
                     /protein_start="280"
                     /score="53.6"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(3696..4481)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21485_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.20E-95"
                     /label="FLN47_RS21485_Condensation_LCL.1"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="264"
                     /protein_start="2"
                     /score="309.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEMIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTDYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(3702..3791)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0004"
                     /evalue="3.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="262"
                     /protein_start="232"
                     /score="51.7"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(4038..4073)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0003"
                     /evalue="4.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.1"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       complement(4104..4169)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0002"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="128"
                     /protein_start="106"
                     /score="29.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4365..4478)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21485_0001"
                     /evalue="5.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="FLN47_RS21485"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     gene            complement(4433..4573)
                     /gene="comS"
                     /locus_tag="FLN47_RS21490"
     CDS             complement(4433..4573)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="FLN47_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_229762786.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECIFSISLDKQTQATTSPLYFCWRE
                     K"
     gene            complement(<4665..7709)
                     /gene="srfAB"
                     /locus_tag="FLN47_RS21495"
                     /pseudo=""
     CDS             complement(<4665..7709)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21495_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     2.5e-115. Score: 376.6. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21495_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value: 8e-112.
                     Score: 365.7. Matches aSDomain:
                     nrpspksdomains_FLN47_RS21495_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1013). E-value: 3.3e-14. Score:
                     44.8. Matches aSDomain: nrpspksdomains_FLN47_RS21495_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="FLN47_RS21495"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 3.7e-92, bitscore:
                     296.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.3e-117, bitscore:
                     378.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.8e-09, bitscore:
                     25.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDE
                     YVNKEYSFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVERDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRS
                     ALDGNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSTK
                     AELPEGMAVHQVFEEQAKRTPASTAVVYEGTELTYRELNAAANRLAKKLVEQGLQKGET
                     AAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTGQA
                     HQLQVPVLTLDIGFEESEAADNLSLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QRISTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADHEVTVSELRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSEAVQPEYAAPKTESEKKLAEIWEGILGVKAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHK"
     aSDomain        complement(4671..4799)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21495_PCP.1"
                     /evalue="3.30E-14"
                     /label="FLN47_RS21495_PCP.1"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="1013"
                     /protein_start="970"
                     /score="44.8"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQEHF"
     aSModule        complement(4671..7691)
                     /complete
                     /domains="nrpspksdomains_FLN47_RS21495_Condensation_DCL.1"
                     /domains="nrpspksdomains_FLN47_RS21495_AMP-binding.1"
                     /domains="nrpspksdomains_FLN47_RS21495_PCP.1"
                     /locus_tags="FLN47_RS21495"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5088..5153)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0010"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5118..6299)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21495_AMP-binding.1"
                     /evalue="8.00E-112"
                     /label="FLN47_RS21495_AMP-binding.1"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="864"
                     /protein_start="470"
                     /score="365.7"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNAAANRLAKKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTG
                     QAHQLQVPVLTLDIGFEESEAADNLSLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRISTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5235..5324)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0009"
                     /evalue="2.70E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       complement(5826..5885)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6072..6113)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0007"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       complement(6639..6755)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0006"
                     /evalue="1.70E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.8"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        complement(6795..7691)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21495_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.50E-115"
                     /label="FLN47_RS21495_Condensation_DCL.1"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="305"
                     /protein_start="6"
                     /score="376.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEY
                     SFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKV"
     CDS_motif       complement(6804..6899)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0005"
                     /evalue="7.30E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7137..7175)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7221..7286)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0003"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7503..7619)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(7623..7709)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS21495_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="FLN47_RS21495_NRPS-COM_Nterm.1"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(7644..7682)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS21495_0001"
                     /evalue="2.30E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="FLN47_RS21495"
                     /protein_end="22"
                     /protein_start="9"
                     /score="17.0"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(7722..>9420)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (22-90). E-value: 1.7e-28. Score:
                     90.6. Matches aSDomain: nrpspksdomains_FLN47_RS17625_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (100-403). E-value:
                     5.5e-180. Score: 591.8. Matches aSDomain:
                     nrpspksdomains_FLN47_RS17625_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (408-562). E-value: 5.7e-56.
                     Score: 180.6. Matches aSDomain:
                     nrpspksdomains_FLN47_RS17625_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="FLN47_RS17625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_17810"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_252187927.1"
                     /sec_met_domain="PP-binding (E-value: 4.5e-19, bitscore:
                     57.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7e-99, bitscore:
                     319.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KALPEPDIEAGSGEYKAPTTDMEELLAGIWQDVLGMSEVGVTDNF
                     FSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPTIEALTQYVERAEGKQADQGPVEGEV
                     ILTPIQRWFFEKNFTNKHHWNQSVMLYAKKGFDPERVEKTLQALIEHHDALRMVYREGQ
                     EDVIQYNRGLEAASAQLEIIQIEGQAADYEDRIEREAERLQSSIDLQEGGLLKAGLFQA
                     EDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERLQDF
                     ANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVH
                     EAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEGHGREEIIEDLTISRTVGWFTSMYPM
                     VLDMKHADDLGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDVSFNYL
                     GQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLEFHEKT
                     VQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKLMEIF"
     gene            complement(7722..>9424)
                     /locus_tag="FLN47_RS17625"
                     /old_locus_tag="FLN47_17810"
     aSDomain        complement(7734..8195)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS17625_TIGR01720.1"
                     /evalue="5.70E-56"
                     /label="FLN47_RS17625_TIGR01720.1"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="562"
                     /protein_start="408"
                     /score="180.6"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7734..9353)
                     /domains="nrpspksdomains_FLN47_RS17625_PCP.1"
                     /domains="nrpspksdomains_FLN47_RS17625_Epimerization.1"
                     /domains="nrpspksdomains_FLN47_RS17625_TIGR01720.1"
                     /incomplete
                     /locus_tags="FLN47_RS17625"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8112..8171)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS17625_0005"
                     /evalue="9.80E-10"
                     /label="NRPS-E6"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="436"
                     /protein_start="416"
                     /score="30.6"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        complement(8211..9119)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS17625_Epimerization.1"
                     /evalue="5.50E-180"
                     /label="FLN47_RS17625_Epimerization.1"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="403"
                     /protein_start="100"
                     /score="591.8"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLYAKKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEIIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       complement(8217..8264)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS17625_0004"
                     /evalue="1.00E-08"
                     /label="NRPS-E5"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="401"
                     /protein_start="385"
                     /score="27.1"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     CDS_motif       complement(8673..8738)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS17625_0003"
                     /evalue="1.80E-12"
                     /label="NRPS-E2"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="249"
                     /protein_start="227"
                     /score="39.3"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       complement(8940..9014)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS17625_0002"
                     /evalue="2.00E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="160"
                     /protein_start="135"
                     /score="20.2"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9075..9104)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FLN47_RS17625_0001"
                     /evalue="3.50E-05"
                     /label="NRPS-E1"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="115"
                     /protein_start="105"
                     /score="16.4"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(9150..9353)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLN47_RS17625_PCP.1"
                     /evalue="1.70E-28"
                     /label="FLN47_RS17625_PCP.1"
                     /locus_tag="FLN47_RS17625"
                     /protein_end="90"
                     /protein_start="22"
                     /score="90.6"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEALTQYVERA"
ORIGIN
        1 ccgtacgtcc aagactggcc gttgtatgtg acagccgggc ggtctttgtg gcgctggaca
       61 gtctcttcga atagctgatg aaccgttttg tctgtcggca caggcagcgc tttgcccttc
      121 catgcctcaa gaagcgcttg cttttctgtc tcggtgacga gtgacaggct ggaaagcggc
      181 tggttcgggt tatccgcaac agcctttagt agccgcagaa cgtattcgct ccatttttca
      241 gctgtttctt tcgcgaacag atctgtcgca tattcgagct gcaagccaat gccattttca
      301 tcagtaaatc cgcccagtgt cagatcaaac ttagaagttc cctgatgagc ctcataaggt
      361 gaaatgctca gattttgcaa tgtaagctgt tctttatcag ggttttctgt cgtcaccatc
      421 acattaaaca atgggtttcg gctcatatcc cgagggagat caagctgatt aaccagatct
      481 tcaaacggat actcctgatg ctcaagcgct gacaggttcg tctgctttgc ttgctccaaa
      541 agctccgcaa atgtttgctg ttctttcgct ttcattctca gggcaagtgt attgacgaac
      601 attcccggca tgtcctgaac atcagcattc gtcctgccgg cagtaacgga gccgatgacg
      661 atatcccgct gtccggaaat tttgcttaaa aagacgtgga aaacagcatg gagcaccatg
      721 tgcaaggtcg ttccggtttc cgcaagcagt ttttctaccg cctttgccgt ctcacttccc
      781 gtacggaaac ggatcgtatt tcctttaaag ctttgcacag gcggacgggc gtaatcgagc
      841 gggagatcaa gctctggcag ctcgcctgaa agaactgaca gccagtacgc ctcatgctcc
      901 ttcatccgtt ctttttgctc agcttcatgt tgccaaacgg cgaaatcttt ataatgaagc
      961 gccggctgcg gaagctgctc tcctttgtaa agcagcgcca attcttttac aaaaatgcct
     1021 cttgagacgc cgtctgcgat gatatgatgc atatctaaaa gcagcaaatg ttttttcgct
     1081 tctattcgaa gcaaagctgc gcgaatgagc ggtgcgtcgt ttaagtcgaa tggccggata
     1141 aattccttaa tcttttggtc cgcttcgtct tctttggctt caaacacatg aagatcaacc
     1201 tccgccttct caatgatgcg ctgaacaggc tcaccattag cttccataaa tgacgtccgc
     1261 aatgattcgt gacggttcac aagctgattg agtgaatcgc gcaggcacga aatatcaagc
     1321 tctccttcca ttagaagaac agatggcatg ttgtagctta tcgtctggcg gtcaagctga
     1381 ttgagcacgt acatccgttt ctgtgcggat gacaatggat atatagcctg cttgtcagcc
     1441 ggtttgagga cttgatggcc gcctgaaacg acagacgcct ccatataagc cgccagttgg
     1501 cgaacagtcg gctgctcgaa cagtgcttta agcggtacgc ttttctccaa ttccgcctga
     1561 atgttggcaa tcagcatcat cccttttaat gaatggccgc ctaaatcgaa aaaattgtcg
     1621 tcaatgccga tggcttctat gccaagcgtt ttctgccaaa tgcggcataa gctttcttca
     1681 agcgctgtct ccggtgcggc caaggctttg cctccggatt gtgccgtgtt cggctttggc
     1741 aaagcgtttc tgtcggtttt tccgtttgcc gtcagcggaa tcgcgtcaac ctgcacaaag
     1801 aaagcaggaa tcatatgaga cggcagtgtt agggaaagct gcttccggag ctctgctttt
     1861 tcaatagctt gatccgacgt ataatacgcg caaagctctt gaagaccgcc ctcggaaacc
     1921 gctagtacaa ccgcttcttg aattccgctg aggctgagca tgaccgtttc aatttctttc
     1981 ggctcaatgc ggtagcctcg gattttcacc tgatcatcag cccggccgat aaatgcaagc
     2041 gtaccgtcag aaagtcttcg tacgacatct ccggttttgt acatgaggct tcccggcgaa
     2101 tatggatttt ctacaaatct ctcggctgtc agctgaggcc tgttgagata ccctctcgca
     2161 agcccctgtc ccgtgatata gagctgtccg ctcgctcccg gcggcacaag cttcaatgct
     2221 tcatttaaga caagcgcgcc ggcattcgca atcgggcgtc cgattgtcgg gcgtttcgca
     2281 aatgcatccg gttcagacag atcaacccgc ccggcgatgg cgccgattgt tgcttctgtc
     2341 gggccgtagt gattgataaa ttcggtatgt ccatacatct tacggaaggc gagaacatca
     2401 gtcgggatga ttttttctcc tcccaagacg atcaagcgca aggattcaaa gttcgcatct
     2461 tttgcaaaac tggcggtgtt cactattgta tggaagagag acggtgtcag tttgatataa
     2521 gtgatcccat gctccttgat atagtgcgct atctcatccg gcgccgtata ggtttccttc
     2581 tggacgatat ggagctcgcc cccgcccaga agtacgggga acatgctcgt atagccaagg
     2641 tcaaatgcgt aagatgaaag caatacagtc ttatcatttt ccgtcaggct cgcctcttca
     2701 ctaaaccaag agacatagtt tgtcagattg cgatgctcaa tctgcacgcc cttcggcttt
     2761 ccggtcgtac cggacgtata aatgacgtat gcaagattgc gagaccctga aatggatgat
     2821 gggcgctcct tgctttcatt tctccattta ccgtcatcaa ggctgacgat tgtcccttca
     2881 tattgcccaa tagccgtcat gtttctttca ttcacgaccg caaattttgc tccgctgtcc
     2941 tctaatgaat aacggattcg ttcttcaggt gtatcaggat caatcggaag gaaagctgcc
     3001 cccgccttca tgacaccgag aatggatacc atgacttcag ctgaccggtc cataacaatc
     3061 gcgacgatgt cttcatttcc tgccccttgg gcacgaagat gatgcgccag ctggttcgcc
     3121 tgttcatcaa gctcgcgata ggtcagcaga tttccgccag ccttcaccgc agaatggtca
     3181 ggtgtttgct gggcctgttt ttcaaaaaga gcggcaaagg ttgcttcggt aatttgagtc
     3241 atatgtcttt cttctagcaa agcagccgtt tctgcagatg aaagcagatc tacatcagac
     3301 agccgaacgt ttggattagc cgcagccgct ttgatgatat tgacaaagtg gcggctccag
     3361 cggcggatcg tctcgtcctt aaacaaggca gtcgcatagt caaagcttag gccgatatcc
     3421 tcttcacgct cgaccgcttc caaggaaaga tcaaatttcg caacatgatg aagcatggaa
     3481 tacgaggaaa tcttcaaatc tcctaatctt aatgaaggaa tctccatatt ctgcatgttg
     3541 aacatcacgc tgaacagtgg acttctgctt gtatcccttg taagcggaag cttttcaatc
     3601 agctcctcaa gcggatagct ctggtgctcg aatgcttgaa ggctggctgt tttgacctct
     3661 tcaaggaatt gcgcgaaggt tttatctccc gctggtgccg ttctgagtgc caccgtgttg
     3721 acaaacatac ccggcacacc ttgcagatca ggatgtgttc tgccagctgt cggcgagccg
     3781 acaatgatat catcctgtga cgcgtacttg gaaaggagta cattgaacgc cgccagcaaa
     3841 aacatgtaca ttgtcgtatc tgtttctgcc atgagtgatt tgatttgagc cgtgatttgc
     3901 ttatcaagcc caaacatcac gcgctctccc gcaaagctcc gttcagcagg ccgctcgaaa
     3961 tccgcgggaa gatccagtat tggcagttcg cctttaaaga catcgagcca gtattcctca
     4021 tctttttgat aatcagtttg ttctttgtgc caaactgcgt aatctttata gtgaatttgt
     4081 ggcagttcca gatctgcgcc ttgatatatt ttggcaagat caccgattag aattcctgtt
     4141 gaacttccgt cagtaataat atgatgcata tcaatgagca gcaggtggcg tttttcttcc
     4201 agctggacaa gcttcgaacg gacgagcggc gcgcggttta attcaaacgg ctgaacaaat
     4261 gctttgatca tctcttccgc gtcttcttcc cgtcccttgg cagcctccag ctggaacgat
     4321 atgtttttat gaacggtttg cacaacctct ccgtcgatca tgtcaaacga tgtacggagg
     4381 atttcgtgcc ggttgattaa ttgctgaatc gcgttttcaa gccggtcttt atctacttct
     4441 ccctccagca gaagtacagc ggggacgttg tagcttgtgt ttgcttgtcc aagctgattg
     4501 agaatataca ttctccgctg ggccggggat acaggataat gctgctgata agatgcttgc
     4561 ctgatcgatt caaacgtctg ctcctccttg ctttcgttct cttccaaata cagtgccagt
     4621 tcttgaatag tcggcttttc aaaaagcact tttatcggaa cttccttatg aaaatgctct
     4681 tgaattttcg ccgtcatcat catcgctttc aaagaatggc cgccgatcat aaagaagtta
     4741 tcggtaacgc ctgctttgac gccgagtatt ccttcccaga tctccgctaa tttcttttca
     4801 ctctctgttt ttggtgctgc gtattccggc tgcacggctt ctgattgcgg tgcaggcagt
     4861 tcctttttgt tgattttccc gtttggcgta agcggcagac tgtccatttg aatcaggtgg
     4921 gcagggacca tatagtcagg caaagactga gatagtgttt ttctcagctc gctcacagtc
     4981 acttcatggt ccgccgtaaa gtacgcgcaa atttcatttt catccgcttt gtttttgcgg
     5041 atgatcaccg cagcctctgt cacatgctca gccatgttca gttttgtttc aatttcacca
     5101 agctcaattc ggaagccgcg caccttcact tggttgtcga ttctgcctaa aaattcgata
     5161 ttgccgtccg gcagccatct tgccaagtca ccagtgcggt aaattctctc acccggtctg
     5221 aacgggtctt cgagaaattg cttctctgtc agctcaggga gattgacata cccacgcgct
     5281 acaccgattc cgcctacgca aagctcacca ggcgcgccga ttggctgcag gcattgctgc
     5341 tcatccataa tataggcagt ggaatttccg atcggcttcc cgattgggat agagccgcca
     5401 tactcgcggt cgatcagaaa actggttgaa aacgtcgtgt tttctgtcgg gccgtatccg
     5461 ttccacaatg aaagcgacgg cgatgcctgt tttactttgc tgacaatatg cgggacaagg
     5521 gcatctccgc cgatgattaa atggcgcagt gtgccgaaca tacccgcatc ctttgcagca
     5581 agctggttga ataaaggtga tgtcagccac atcgtcgaga ttctttgctc acgcagaaat
     5641 gcagcaaatt gtttcgcatc aagcagcgtc tcttttttga ccggataaag cgctgcgcca
     5701 ttcagcagtg caccgaatac ctcaaacgtt ccggcatcaa agctgacagc tcccgtttgc
     5761 gccatgcggt cctcttcagt aacaggaacg tacccggcat ttttgacgag gcgcagaatg
     5821 cttttatgtt cgatcatgac gcctttcggt ttgcccgttg aaccggatgt gtacatgatg
     5881 tacgccaaat cagacggggc ggatggcagg ctgagattgt cagccgcttc gctctcttca
     5941 aagcctatgt ccagtgtaag aaccggcact tgcagctgat gtgcctgccc tgtataagaa
     6001 tttccggtca aaaccattcg aacggagctg tcctctgcca tgaaacgaag acgatcccca
     6061 ggaagcgctg gatcaagcgg cacatatgcg gcgcctgctt ttaacacagc cagcatgccg
     6121 acaacggttt ctactgagcg gtcgttcata atcgctgctg tttccccttt ttgaagccct
     6181 tgttcgacaa gctttttcgc caggcggtta gccgctgcat ttagctcgcg gtacgtcagc
     6241 tcggttcctt catatacgac ggcagtgctc gccggcgtcc gtttcgcttg ctcttcaaac
     6301 acctgatgaa cagccattcc ttcagggagt tcggctttcg tgctgttaaa cacctcgaca
     6361 atttgacgct gctcttcgtc tcccacaagc ccgtactcac gcacaaaagc ggccggctga
     6421 tcgacagctg cttccatcat gcgggtaagg tgctccgctg tccgttcgat aaaagcggaa
     6481 tcaaaggcgg ctccgttata tttaattttg acggtccacg ttttgcccgg atacacaatc
     6541 aggttgaaat cgaagctcgt ttgttcaaat gcgctttcta ccttaatcga aaacccgagg
     6601 cggtcttcca tgctgccgtt ttcaagctct tgatcaagcg gataattttc aaacgcgacc
     6661 agatggttta agagattgcc atcaagggct gagcgttttt gaatctcata gagcggcacg
     6721 taatcgtaac gctctgcctc tactgcatgc tgctgaacag ctgtgaaaat atcagcgaat
     6781 gcagcatctc gttcaacttt cacccgcacc ggaatggtgt tgataaacag ccccgccatc
     6841 gtttcaattc cgttgatttc agacggcctt cctgagacga ccgttccgaa gacaacatca
     6901 tccgtaaagt tgtatttgct gagaacaatg ccccaaacgg cctgaaatag gttaggccct
     6961 gtcacttgat ggagatttgc ggtttgctga atacgggcaa ccaatgtttc gtcccatgta
     7021 aaggaatatt ctttattgac gtattcgtct ttttttacag gcaggcggcc cgggagcacg
     7081 cttggctgtt caaaacccgc caagcgctcg ctccagtagc tctccgcttc ttcattgtcc
     7141 tgttttccaa gccatttgat gtagtcggag taaggcttgc cttgaccgtt tgcaggtgtc
     7201 cttcctgctc tgagcgcttc atagttttgg aacaggcttt tcatgaggac gcccatgctc
     7261 cagccgtcca tcataatatg atgattgctc cagacaagat acagctgatc tgcatcaagg
     7321 cggaaaaccg ctaccttgaa caatatgtct tttgccaggt taaagccttg gcgctgaacg
     7381 tcttgtttgt aacgctcaat gtgctctttc tggcggttct cgtctgcatg actgatatcc
     7441 tcataatgca cctttgtttt gcgttccgct aacacgacct gacgcggttt ttgcagctgc
     7501 tgatgcacaa ataccgtacg gagaatatcg tatgaccgaa tcagttcatt gacgcttttc
     7561 tcaaagattt caagatcaaa agcgccgtga atcccaagct ctaattgtgt gaagtacgag
     7621 gaagaatgcg gatcaagcat cgcatgatac agcattccct cctgcattgg tgtcagtgcg
     7681 tacacctttt gaatcgattt tttgctcata tgccacctct attagaaaat ttccattaat
     7741 ttatccagct catccagcgt caaatcgtcg tcaccaagat cactcggggt cagttccgta
     7801 ccgtcttgag ataggcaatg ttcaatgatt ctgagaagat gcgctttaaa gctgtcactg
     7861 aatgtttgta ctgttttttc atgaaattca agagaatgat aggccagtga catcgtcagt
     7921 tttccgtttt cgatatatcc gacaacatcc agcgcattcg gtttttctgt ttcagggctt
     7981 aacgactgtc ctgatggcag ctctgatctc gtaaacaaac ctgcatccga catttcgtca
     8041 aactgaccta aatagttgaa gctgacatcc ggctgaatcg agaacgccac atcttcttta
     8101 tgttccggtg ccgtcagata gcgcagaatg ccgtatccga ctcccttatt cggcacatgt
     8161 ctgatatctt ctttcatttg cttcagctgg tagcccagat catccgcatg cttcatatcg
     8221 agcaccattg ggtacatact tgtaaaccag ccgactgtgc gggaaatggt cagatcttct
     8281 atgatttctt cacgtccatg cccctctaaa tgaacactta ctcgatcctg acccgtccac
     8341 tccttcattg ccaatccgaa tgccgtcagc aaaatatcat tcatttctgt gtgatatgcc
     8401 tcatgcacct ttgtagtgag ctgttctgtc tcttcagcag tcagcgagaa ttcaattgtc
     8461 tttgtatgtt tcattcgctg atcaccagat tcgcggtctt tcggaagctt tgccgcgaca
     8521 gcttgttctt caagctgtct ccaatactct ttttctttca aaaaggcttt ggagttcgcg
     8581 aagtcttgca atctctctgc atactctgca aatgaatgtg ttttctgcgg gagaaccggt
     8641 tcattgcctt gctcaagctg tgtatatact gcggcgaaat cctccagtaa aatccgccac
     8701 gacacgccgt caaccactaa gtggtgaatg gcaagaagca agtggtctcc gtcttccgct
     8761 tggaacaagc ctgcttttaa caagccgcct tcctgcaagt cgatgctgct ttgcaaacgc
     8821 tccgcttctc tctctatacg gtcttcgtaa tctgcagctt ggccctcaat ctggatgatc
     8881 tccaattgag ctgaagcagc ttcaagacct ctgttgtatt gaatgacgtc ttcctgtccc
     8941 tcacggtaga ccatacggag cgcgtcatga tgctcgatca gcgcctgcaa tgttttctcc
     9001 acccgttcag gatcaaagcc ctttttggcg taaagcatca ccgattggtt ccagtggtgc
     9061 ttgttcgtga agttcttttc aaagaaccag cgctggatcg gcgtcaggat gacttcgccc
     9121 tccaccgggc cttggtctgc ctgtttgcct tcggcacgct ctacatattg ggtaagcgcc
     9181 tcgatcgtcg ggtgctggaa gagatctttc atttccagct tccagccgtg ctggttcaag
     9241 cggctcgcca tttgaattcc tttgatggaa tctccgccaa gcgagaagaa attgtcggtg
     9301 acaccgactt cagacattcc aagcacgtcc tgccagatgc cggcaagcag ctcttccatg
     9361 tcggtcgtcg gcgctttgta ttctccgctt cccgcttcga tgtccggctc aggcaaggct
     9421 ttgc
//
