LOCUS       NZ_VIOH01000001        20747 bp    DNA     linear   CON 03-JUL-2024
DEFINITION  Bacillus subtilis strain FH1 NODE_1_length_1068410_cov_135.161835,
            whole genome shotgun sequence.
ACCESSION   NZ_VIOH01000001
VERSION     NZ_VIOH01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1068520)
  AUTHORS   Khan,M.A., Khan,M.N., Imran,M., Hameed,A. and Hasan,F.
  TITLE     Draft genome of Bacillus subtilis FH1 isolated from tannery waste
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1068520)
  AUTHORS   Khan,M.A., Khan,M.N., Imran,M., Hameed,A. and Hasan,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2019) Department of Microbiology, Quaid-I-Azam
            University, Facutly of Biological Sciences, Islamabad, Federal
            45320, Pakistan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012028945.1-RS_2024_07_03
            Annotation Date                   :: 07/03/2024 03:57:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,286
            CDSs (total)                      :: 4,159
            Genes (coding)                    :: 4,053
            CDSs (with protein)               :: 4,053
            Genes (RNA)                       :: 127
            rRNAs                             :: 10, 16, 11 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 7, 16, 11 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 62 of 106
            Pseudo Genes (incomplete)         :: 50 of 106
            Pseudo Genes (internal stop)      :: 18 of 106
            Pseudo Genes (multiple problems)  :: 21 of 106
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:36:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 614464
            Orig. end                         :: 635211
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VIOH01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="3"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            1631..4504
                     /gene="uvrA"
                     /locus_tag="FLN47_RS03095"
                     /old_locus_tag="FLN47_03125"
     CDS             1631..4504
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="FLN47_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03125"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_003228057.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVAPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALAFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
     gene            <4585..5148
                     /locus_tag="FLN47_RS03100"
                     /old_locus_tag="FLN47_03130"
                     /pseudo=""
     CDS             <4585..5148
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391416.1"
                     /locus_tag="FLN47_RS03100"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03130"
                     /product="flagellin"
                     /pseudo=""
                     /transl_table=11
                     /translation="RFRSELTVQAGNTGTQQAEDLGAIKDEMDALIEEVDGISNRTEFN
                     GKKLLDGTETDGFTFQIGANAGQQLTVNIDSMSSTALGVNALNVTDFANTPFDTQLESI
                     DTAINNVSNQRAKLGAVQNRLEHTINNLGASSENLTAAESRIRDVDMAKEMSDFTKNNI
                     LSQASQAMLAQANQQPQNVLQLLR"
     gene            complement(5347..5577)
                     /locus_tag="FLN47_RS03105"
                     /old_locus_tag="FLN47_03135"
     CDS             complement(5347..5577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="FLN47_RS03105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03135"
                     /product="hypothetical protein"
                     /protein_id="WP_003235419.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIHEQKRMNREMIAELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    5546..5561
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            5795..6121
                     /gene="yvlA"
                     /locus_tag="FLN47_RS03110"
                     /old_locus_tag="FLN47_03140"
     CDS             5795..6121
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="FLN47_RS03110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03140"
                     /product="protein YvlA"
                     /protein_id="WP_032726939.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LREA"
     gene            6146..7243
                     /gene="yvlB"
                     /locus_tag="FLN47_RS03115"
                     /old_locus_tag="FLN47_03145"
     CDS             6146..7243
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="FLN47_RS03115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03145"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_014478112.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            7248..7445
                     /gene="yvlC"
                     /locus_tag="FLN47_RS03120"
                     /old_locus_tag="FLN47_03150"
     CDS             7248..7445
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="FLN47_RS03120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03150"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            7447..7806
                     /gene="yvlD"
                     /locus_tag="FLN47_RS03125"
                     /old_locus_tag="FLN47_03155"
     CDS             7447..7806
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="FLN47_RS03125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03155"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(7834..9045)
                     /gene="pchE"
                     /locus_tag="FLN47_RS03130"
                     /old_locus_tag="FLN47_03160"
     CDS             complement(7834..9045)
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="FLN47_RS03130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03160"
                     /product="MFS transporter"
                     /protein_id="WP_003228068.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(9066..9575)
                     /gene="pchR"
                     /locus_tag="FLN47_RS03135"
                     /old_locus_tag="FLN47_03165"
     CDS             complement(9066..9575)
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="FLN47_RS03135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03165"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_003228073.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     misc_feature    9857..9872
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     misc_feature    complement(9859..9873)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(9860..9875)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            10001..10747
                     /gene="pchC"
                     /locus_tag="FLN47_RS03140"
                     /old_locus_tag="FLN47_03170"
     CDS             10001..10747
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="FLN47_RS03140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03170"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_021480699.1"
                     /sec_met_domain="CDPS (E-value: 4.9e-84, bitscore: 277.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEPLTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFMDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITCEG"
     gene            10763..11980
                     /gene="cypX"
                     /locus_tag="FLN47_RS03145"
                     /old_locus_tag="FLN47_03175"
     CDS             10763..11980
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="FLN47_RS03145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03175"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_021480698.1"
                     /sec_met_domain="p450 (E-value: 7.8e-37, bitscore: 123.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKIDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEDFCYAESGLYTRGPVSLH
                     IVFDRA"
     gene            complement(12019..12480)
                     /gene="pchA"
                     /locus_tag="FLN47_RS03150"
                     /old_locus_tag="FLN47_03180"
     CDS             complement(12019..12480)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pchA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="FLN47_RS03150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03180"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_072175110.1"
                     /transl_table=11
                     /translation="MNNSIHDELFQAIQQFALKRDKRVWQKVQIPSSQHLDHLKKDWTL
                     TQLHIVSCLHTSKNVNNSFLASRLHISKAAVSKAIQALLKHNIITVTKKPGNKKEIFYT
                     LTESGKELAGLHERLHQKAKEQYKQLFNKFSIDELKTVTAFINLWIKHM"
     gene            12645..16514
                     /gene="yvnB"
                     /locus_tag="FLN47_RS03155"
                     /old_locus_tag="FLN47_03185"
     CDS             12645..16514
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="FLN47_RS03155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03185"
                     /product="metallophosphoesterase"
                     /protein_id="WP_072175109.1"
                     /transl_table=11
                     /translation="MRKYTVIASILLSFLSVLSGGHHESKAFPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRNNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESEMKKLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIFNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTEGRADRIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERIFKTEREHPDQPEVIDSEADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPESETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRVFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDQDIAWMDQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            complement(16534..17265)
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="FLN47_RS03160"
                     /old_locus_tag="FLN47_03190"
     CDS             complement(16534..17265)
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="FLN47_RS03160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03190"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_072175108.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATMEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(17283..18011)
                     /gene="nagB"
                     /locus_tag="FLN47_RS03165"
                     /old_locus_tag="FLN47_03195"
     CDS             complement(17283..18011)
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="FLN47_RS03165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03195"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_064671535.1"
                     /transl_table=11
                     /translation="MKVMECQTYEELSQKAARITADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYARLSSNDPNSYHFYMNDRLFQRIDSKPSRHFIPNG
                     NADDLEAECRRYEQLVESLGGTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIRTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            complement(18008..19198)
                     /gene="nagA"
                     /locus_tag="FLN47_RS03170"
                     /old_locus_tag="FLN47_03200"
     CDS             complement(18008..19198)
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="FLN47_RS03170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03200"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_072175107.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-42, bitscore:
                     142.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTELPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSALDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKEESIIASMGHTDADSAMLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     misc_feature    complement(19253..19268)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            19380..20312
                     /gene="hprK"
                     /locus_tag="FLN47_RS03175"
                     /old_locus_tag="FLN47_03205"
     CDS             19380..20312
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="FLN47_RS03175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLN47_03205"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003228097.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
ORIGIN
        1 ctgagacact ccgccacatc agctttgttt gagcgcagag atgtcattat tattgcgagt
       61 gtgtcttgta tatacggcct cggttcgcct gaggaatacc gggaaatggt cgtgtcactg
      121 cggactgaaa tggaaattga gcgcaatgag ctgctcagaa aacttgtaga catccaatat
      181 gcccgcaacg atatcgactt tcagcgcggt acatttcgtg tgcgcgggga tgtagtggaa
      241 atctttccgg catcccgtga tgagcattgt atcagagttg aatttttcgg tgacgaaatc
      301 gaacggattc gggaagtgga tgcgctgaca ggagaaattc tcggcgaccg agatcatgtt
      361 gcgattttcc cggcatccca cttcgtaacg cgggccgaga aaatggagaa ggcgattcaa
      421 aatatcgaga aggagcttga ggagcagctg aaggtcatgc acgaaaacgg aaagctcctt
      481 gaagcgcagc gtttagagca gcggacaaga tatgatcttg aaatgatgcg tgaaatgggt
      541 ttctgctccg gcatcgagaa ctattcaaga catttgactc tgcggcctcc aggttcaacg
      601 ccgtatacgc ttcttgatta tttcccagac gattttatga tcgtggtgga tgagtcgcat
      661 gtgacaattc cccaggtgcg cggcatgttt aacggagacc aggcgcggaa acaggtgctc
      721 gtggatcacg gcttccggct tccatcagcg cttgataacc gtccgcttcg ttttgaggaa
      781 tttgaaaagc atatgcacaa tatcgtgtat gtatcagcaa caccagggcc gtacgagatt
      841 gaacatacag atgaaatggt cgaacagatc attcgtccga cggggcttct tgacccgctt
      901 attgacgtcc gtccaatcga aggtcagatt gacgacttga tcggcgaaat tcaagcaaga
      961 atcgagcgaa atgaacgggt gcttgtgaca actttgacga agaagatgtc agaggatctg
     1021 accgattatt taaaggaaat cggcattaaa gtaaactatc tgcattctga aatcaagacg
     1081 cttgaacgga ttgaaattat ccgcgacctg cgccttggca agtacgatgt gcttgtcggc
     1141 atcaacctgc tgagggaagg tttggacatt cccgaagtat cccttgttgc gattttggat
     1201 gcggataagg aaggtttcct tcgttcggag cggtcgctga tccagaccat cggacgggca
     1261 gcgagaaatg cggaaggccg cgtcattatg tacgccgata aaataacgaa gtctatggaa
     1321 attgcgatta atgaaacaaa acgccgccgc gagcagcagg agcggtttaa tgaagagcac
     1381 ggcattacgc cgaaaacgat aaataaagaa attcgcgatg tcattcgtgc cacagtagca
     1441 gctgaggata aagcggaata caaaacaaaa gccgcgccga agctgtcgaa aatgacgaag
     1501 aaagaacgcc agaaagtcgt tgaacagatg gagcatgaaa tgaaggaagc cgccaaggcg
     1561 cttgactttg agagagccgc ggagcttcgc gatttacttt tagagctaaa agcggaagga
     1621 tgatgaacaa atggctatgg atcggataga ggtgaaggga gccagggcgc ataacctgaa
     1681 aaatatagat gtaacgattc cgagagatca gcttgtcgtt gtcacgggtt tgtccggatc
     1741 aggtaaatcc tcccttgcct ttgacacgat atatgctgaa ggacagagac ggtatgtcga
     1801 gtcgctgtct gcttatgccc gccagttttt agggcaaatg gataagcctg atgtggatgc
     1861 aattgagggg ctctctcccg ccatcagcat tgatcagaaa acaacaagcc gtaatccgag
     1921 gtctactgtc ggtacggtaa ctgagattta tgattatctg cgtcttttat atgcgagagt
     1981 agggaagcct cattgtccgg agcacggaat tgagattaca tcccagacca tcgagcaaat
     2041 ggtggacaga attctggaat acccggaacg gacgaagctt caggtgctgg cgccgattgt
     2101 ctcgggccga aaaggcgctc atgtcaaagt gcttgaacag attagaaaac aaggctatgt
     2161 cagagtcaga attgacggcg agatggctga gctttccgac gatatcgaat tagaaaagaa
     2221 caagaagcat tccattgagg tagtcattga ccggattgtg gtgaaagaag gcgtggcagc
     2281 ccggctgtct gattcattgg aaacggcgct ccgtttaggt gaaggacggg ttatgatcga
     2341 tgtcatcggt gaggaagagc tgatgttcag cgagcatcat gcctgtccgc actgcggatt
     2401 ttcaattggt gagcttgagc cgcgtctgtt ttcgtttaac agtccgttcg gggcgtgtcc
     2461 gacgtgtgac ggtctcggaa tgaagcttga agtggatgcc gatcttgtca ttcccaatca
     2521 agatttgtca ttgaaggaga atgcggtcgc cccttggaca ccgatcagct cacaatatta
     2581 tcctcagctg cttgaggcag tctgcaccca ctacgggatt gatatggatg tgccggtcaa
     2641 agatttgccg aagcatcaac tggataaagt gctgtacggc agcggagatg acctgattta
     2701 tttccgatat gaaaatgatt ttggacaaat ccgcgaaggt gaaattcaat ttgaaggcgt
     2761 attgcgcaac attgaaagac gctataagga gacaggctcc gatttcatac gtgagcaaat
     2821 ggagcagtat atgtctcaga agtcttgtcc gacgtgcaaa ggctatcggt taaagaaaga
     2881 ggcgcttgcc gtactgattg acggccgcca cattggaaaa attaccgagc tgtctgtcgc
     2941 cgacgcactt gccttcttta aagaccttac cctttctgag aaggatatgc agatcgccaa
     3001 tttgattttg cgcgaaattg tggagcgctt aagctttctg gacaaagtcg gcctcgatta
     3061 cctgacattg agcagggcag cgggtacatt gtccggggga gaggcacagc gcatcaggct
     3121 ggcgactcaa attggctcgc gtttatccgg tgtgctttat attttagatg agccgtctat
     3181 cggtctgcat cagcgtgata acgaccgctt gatcagcgct ctgaaaaata tgagagacct
     3241 cgggaacacg ctgattgttg tcgaacatga tgaggacacg atgatggcag cagattattt
     3301 aatagatatt ggaccgggag ccggcattca cggcggacag gtgatatctg cgggtacgcc
     3361 ggaagaagtg atggaagatc caaactcatt aacgggcagc tatttatcag ggaaaaagtt
     3421 tatcccattg cctcctgaaa gaagaaagcc ggacggacgt tacattgaaa ttaaaggtgc
     3481 ttcagaaaac aacctgaaaa aagtgaatgc caagttcccg cttgggacgt ttacagcagt
     3541 tacaggtgtt tccggttcag gaaagagtac actcgttaat gaaattttgc ataaggcgct
     3601 ggcgcaaaag cttcataaag cgaaagcgaa gcccggcagc cataaagaga ttaaaggttt
     3661 ggatcattta gacaaagtca ttgacattga ccaggcgcca attggaagaa cgccgagatc
     3721 caaccctgcg acatacaccg gtgtatttga tgacattcgt gatgtattcg cgcagacaaa
     3781 tgaagcgaag gtccgcggct ataaaaaagg ccgtttcagc ttcaacgtga agggcggacg
     3841 atgtgaagcc tgccgcggag acgggattat taaaattgaa atgcacttcc ttcctgacgt
     3901 atacgttcca tgcgaggtgt gtcacggcaa acgctataac cgtgaaacgc ttgaagtgac
     3961 gtacaaagga aaaagcatct ctgatgtgct tgatatgacg gttgaagatg ctctttcttt
     4021 ctttgaaaat atcccgaaaa tcaaacgcaa gctccaaacc ctttatgatg ttggtttagg
     4081 ttatattacg ctcggccagc cggcgacgac cttgtcaggc ggagaagcgc agcgcgtgaa
     4141 gctcgcgtca gagctgcaca aacgctcgac cggacgcacg ctctacattt tagatgagcc
     4201 aacgacaggt ttgcatgtcg acgatatcgc caggcttctt gtcgtgctgc aacggttggt
     4261 agacaacgga gacactgtac ttgttattga gcacaacctt gatatcatta agacagccga
     4321 ttacattgtg gatttgggcc cggaaggcgg agccgggggc ggaaccattg tcgcgtcagg
     4381 aacgcctgag gaaatcactg aagtggaaga atcatataca ggccgttatt tgaagcctgt
     4441 tatcgaacgt gacaaaacac gcatgaaatc gctcttgaaa gcgaaagaaa cagctacatc
     4501 ttaaaaccct ctgttaagag gggacagctt gtcagcaagt ccatccttgg gcttagcagg
     4561 caagcttttt ctttacggca taataggttt agaagtgaac ttactgttca agcaggaaac
     4621 acaggtacac aacaagcaga agatcttggt gcaattaaag atgaaatgga tgcgcttatt
     4681 gaggaagttg atggcatttc aaatcgtact gaattcaacg gcaaaaagtt attagatggt
     4741 actgaaactg atggtttcac tttccaaatt ggagcaaatg ctggccagca gcttacagtt
     4801 aacattgaca gcatgtcatc aactgcttta ggagtaaacg cacttaatgt aacagatttt
     4861 gctaatactc cttttgatac tcaacttgaa agtattgata ctgcaatcaa caatgtctca
     4921 aaccaacgtg ctaaacttgg tgcggtacaa aaccgtctag agcacacaat caacaacctt
     4981 ggtgcttctt ctgaaaactt aacagctgct gagtctcgca tccgtgacgt tgacatggcg
     5041 aaagaaatga gcgatttcac aaagaacaac attctttctc aagcttctca agcgatgctt
     5101 gctcaagcaa accaacagcc gcaaaacgtg cttcaattgt tacgttaatt ttgaacggct
     5161 atctctttct gatagttaca gcttgccgac agagtccatt aactggatgt gccggcaggc
     5221 tttttttgtt acaatcattt tgtgacagcc agggaatcac ttgttttttc aaaacaaaaa
     5281 aggcaagata ttgtcatcta acctttaact ttgtttcagt tataatgaat gatatgtttt
     5341 ccgcaattac aaagtcttct tatacagctg cttacttaca ctcattaaat cttgaacaat
     5401 atgcgtgttg ctgctgtcga gcagcacttt tatatattgg atatcgtgag agatcgggaa
     5461 gggtccgtga atctgctcaa gctcggcgat catttcacga ttcattcttt tttgttcatg
     5521 aatcagcttt aacactgatt cttgtgttac tgtatagtca ctgggatctt tctccattgc
     5581 ttgtccagcc tccttggttg ccgtaagctt tttacatatt gtaatcggta tagccggaag
     5641 attctacttc taaaactttt taaaatccaa tttccgtaaa tcggagcatg tctaaagaat
     5701 gaatttgaaa cctgaagaga ttttaaacgt ataaataagt aaaacgtccc tgatcttttg
     5761 tcatggacaa ttcacaatag gagtgtggaa tgaattgaac cgtaatcaag ctattattgc
     5821 atcactttgt tatttcagtg tgtttattgc gccaatcatt gtgccgattg tcgcttattt
     5881 tgttgtaaat gagaaagaaa caaaacgcca tgcgatacgc tctcttattt ctcacatagt
     5941 tccgtttgtc ggctggctgt tcctttttat tgctttactt ggcggagccg ttgcgattga
     6001 tggagacagc ttgcttcctg tatttgtaat catcggggga gcggttattt atttcttagt
     6061 tgtcattggc attatcattt ggaatgtgat tcagggcatt aaggtcctgc gtgaagcata
     6121 agtagacaat ggggaggaga tcgtaatgaa gcaagaaaag gaacgaatcc ttaagcttgt
     6181 tgaggaagga aagcttacag cacaagaagc tttgacactg attgaaaagc ttgacagcga
     6241 ctacaaggaa aaagaagaga aaatcacagc gctctcagtt cacgttcatg acgaggaaga
     6301 gccatttaca acggcaaaaa aagaaagcgg caagccgtct ttaggagcca agctgtttga
     6361 ctggattgat tctgctgtaa aaaaggtcaa ggaagttgac cttgatttaa actttggcca
     6421 tgcgtatgat gttcagcaca tttttcaatt taaagatact gacttttcca gtgttgagct
     6481 acaaattgcc aatggcagcg tgaatatcgt tccttgggaa gatgacgata tcagagcgga
     6541 atgccaggcg aaggtatatc gcgctgatag ccaggatgcg gcgagacacg catttcttca
     6601 gcatattgaa tgtgaaatca aaggaaataa gttttttatc cgtactgaga aaaagacgat
     6661 gaaaacgaat gtcactttgt atattcctca gaaggagtat gataagattc gagtgaagct
     6721 gtttaacggt ccggtcagag gggaacattt acatgtaaaa gagttctcag cgaaaacgac
     6781 aaacggcgtg ctgtcgttct cgcacttaac agccgaaaaa gccatagctg aaacggccaa
     6841 cgggcaaatt aagctcgcca gccacagctg cggaacgata gaagcggaaa ccattaacgg
     6901 attgatcgat ctcagaggga aaagcgaatc cattgacgtt caaagcttta acggaaatat
     6961 tgcgattaac gtaacagaat cagactgccg ctccatttat gcaaaaacaa cgactggaag
     7021 tgtggagctt gcgattccgg acgatctcgc agtgaaggca gagctgaaaa gcaatcttgg
     7081 ttcgctgtcc catgaattaa tggatgttga gatgctgaag gaaaaaaatg ataccattca
     7141 aaaagaaatg atgttcactt caaatcaggc acatgatcaa aacatcaccg tcttctcaga
     7201 atcactaaca ggcgccatca aactaaagta ctcacagagg tgatcgaatg aataagcttt
     7261 atcgctcaga aaaaaataag aaaatcgccg gtgttatcgg aggtctcgcg gaatatttca
     7321 attgggacgc atcactcctc agagtcataa ctgtcatctt agccattatg acaagtgtct
     7381 tgccggtttt gctcatttat atcatttgga tttttatcgt cccgtcagaa agggatatga
     7441 aatgacatgg taaaatgggc agtcagcatt ttagtcaatg cattgttatt aatcgtcatt
     7501 gatggatata ttgactccat ccatatcagc agcattgggg cagcgattat cgccagcctc
     7561 attttatcga tcttaaacgt gctgattaaa ccgcttttaa ttatctttac actgccggtc
     7621 accatggtga cgcttggact cttcttgttt gtgattaacg ccatcacgct tatgatgacc
     7681 gcgtccatta tgggtgacag ctttcaaatc gacggcttcg gcacagcgat atgggcatcc
     7741 gttatcctgt cggtcttcca tttgctgatt caaaaaggca ttttagagcc gcttagaaaa
     7801 aaataaaaaa agctgcccgc aaaggcagct tttttacatg tggtgctttt tttgtttcgt
     7861 gcgcttctgc ggcgaaaaca ttggcggaag aaggcaaaag ctcaatgcgg caaatccaag
     7921 gcctaggagg ctaaaggaca tggcagacgg catcattgtg agcaagtagg cagataccat
     7981 cggacctgct gccatgcctg tatatcggat aaagttaaac attccgattg cactccctct
     8041 gttgtgctca aattcattag taatcattgt agagtacaag ggaggaataa cccccatcga
     8101 gatgccaaac agtgccagcg tcagagccat gagaacgaga gaaacggaat gtgtaacagc
     8161 aaataaaatg atgctgcagg cggcaatcac attgcttccg ataaacaagg tgtgcagccc
     8221 gatttttgcc tgtattcttt taaacagaaa cgtacctgca atcgtgctca gcgccagcgg
     8281 caaatataac agtcctgcta tacccacatc tatatggtaa tgctctgtca gcaatattgg
     8341 gaggtagaca attactgcaa aataaatgaa aaagagaaca aaacttaaag tcaaaatgac
     8401 gttccctgtt cgatttgtaa atattgattt ataatgagcg aacacattgc ctttggcttg
     8461 ctgcatagat tcagttggag aatctttagg aaaggtgatg ctgtttgtca ccagcaaaac
     8521 gatcgagatg gccgcaagta tccaaaaaat cccttcgtat ccggctgctc cgcctatgaa
     8581 tcctcctaga actggggcaa tagccggtgc gacagacagg agcatttgat acgttcccat
     8641 tgcactcccg cgttcattgc ctgtaaacag ctgtccgatc gttgtggcag caataagagg
     8701 cagtgctgcg gaaccggctg cctgtatcat tctgaatatc agaaacaaag taaagtcagt
     8761 agtcaccgca cagccgatgc tggctgctgc cgttgccaga ataccggtaa tcaagacgat
     8821 ccgagcgcct ttaaaatcaa tgatcgctcc taatataatt tgcattattg ctgtcacaat
     8881 cataaaaact gagactgaca ggttcaccat agctgtggaa acatggaatg attctttaat
     8941 gatcggaaga ataggtgaat aaatgttctg gcttaaagat gcaaaaaatg cactgaagca
     9001 aacagtgtat aagatcaata ctgcctttga ttttgtatga gccaaagtgt acaccgcctt
     9061 tttgtttact ttacaggttt gtccggagtc ccatcggttt gttctgcttc aagtgttgac
     9121 gtcaactgct ctagaaactt caatacagcc ttttgttctt cttgtgaaaa tgaatcgagg
     9181 aattggtaaa aagccagctc ctttttttta tgcagtttct catgtaagtc gaaaatccgt
     9241 ctgcctttac gggttaattt aaaataaact tcttttttgt tatctgtcag ctgatagctg
     9301 ttaatgaatt cttccttgat cagttttgtg ctgattttcg ttacattcgc tttcgataag
     9361 ttcatttttc tggcaatccc cgcattgtta atgggttcat gctggccgat gcagtcaatg
     9421 acgtgaatgc ttgtcatatt tttgggaatt tcagctacat cctcttctgc ggcctcctta
     9481 aaaaattgct gaagtgaagt gttcgctttt tgttcattaa ggtgcagcag tctcacgtat
     9541 atgtcgtata tcatctgttt tgtcaaatca gacataggct accttctttc ttaggaataa
     9601 tagtttacga tgaaacaata aaacaaatcg tttactggtg aacaatttta ttttatcttt
     9661 tcttgttttc gttgtcaatt gttggtgttt agactatttg ttgatatgtc atgctgaata
     9721 tgcgtgtaaa aagtgtgtgg aaaatgtgat gtttacattt attttcaaat atttgggcac
     9781 agatcatatt gacgtttgga aggaaaaggg ataaaatgac taatgtttac tagtaaacat
     9841 ttatcatgat atgggtattg aaaatgatta ttaaaatctt aaaaaacatt tggaaacttt
     9901 aagtgatgcc tgccattcgt cttgttcagt tgttctagct attccatctt gtatttgagg
     9961 gtgatgactt aaagcgggat aagttttagg ggtgaatgag atgaccggaa tggtaacgga
    10021 aagaaggtct gtgcatttta ttgctgagcc attaacggaa aactgcagag aaatatttga
    10081 acggcgcagg catgttttgg tggggatcag cccatttaac agcaggtttt cagaggatta
    10141 tatttacaga ttaattggat gggcgaaagc tcaatttaaa agcgtttctg ttttacttgc
    10201 ggggcatgag gccgctaatc ttctagaagc gcttggaact ccgagaggaa aggctgaacg
    10261 aaaagtaagg aaagaggtat cacgaaacag gagatttgca gaaagagctc ttgtggctca
    10321 tggcggggat ccgaaggcga ttcatacatt ttctgatttt atggataaca aagcctacca
    10381 gctgttgaga caagaagttg aacatgcttt ttttgagcag cctcattttc gacatgcttg
    10441 tttggacatg tctcgtgaag cgataatcgg gcgtgcacgg ggcgtcagtt tgatgatgga
    10501 agaagtcagt gaggatatgc tgaatttggc tgtggaatat gtcatagctg agctgccgtt
    10561 ttttatcgga gctccggata ttttagaggt ggaagagacg ctccttgctt atcatcgtcc
    10621 gtggaagctc ggtgaaaaga tcagtaacca tgaattttct atttgtatgc ggccgaatca
    10681 agggtatctc attgtacagg aaatggcgca gatgctttct gagaaacgga tcacatgtga
    10741 aggatgatag ggggagtaaa acatgagcca atcgattaaa ttgtttagtg tgctttctga
    10801 tcaatttcaa aacaatccat atgcttattt ttcacaactg cgggaggaag atccggttca
    10861 ttatgaagag tcgatagaca gttattttat cagccgctat catgatgtcc gctatatcct
    10921 tcagcatccg gatattttca cgacgaaatc acttgttgag cgtgccgaac cagtaatgcg
    10981 aggccctgta ctggcccaaa tgcatggaaa agaacactct gccaaaagaa gaattgtagt
    11041 gagaagcttc atcggtgacg cactggatca tctgtctcca ttgattaaac aaaatgcaga
    11101 aaacttgtta gcgccctatc ttgaaagagg gaaaattgat ctcgtcaatg attttggaaa
    11161 gacgtttgcg gtgtgcgtca cgatggacat gctcgggctg gataaaagag accatgaaaa
    11221 aatctctgag tggcacagcg gagttgccga ttttatcacg agtatctctc aatctcctga
    11281 agcgcgggca cattcgttat ggtgcagcga acagctttcc caatacttga tgccggtcat
    11341 tgaagaacgt cgcgtcaatc cgggatcaga tttaatttcc atcctatgta cttctgaata
    11401 tgaaggcatg gcgatgtcgg acaaggatat actcgcactg attcttaatg tgctgttagc
    11461 cgcaacggaa ccggctgata agacgctggc actgatgatc taccatttgc tcaacaatcc
    11521 tgagcagatg aatgatgttt tggctgaccg ttcgttagtt ccgagagcca ttgcggagac
    11581 attgcgttat aaaccgccgg ttcagctgat tccgcggcag ctgtcccaag atacagtggt
    11641 cggcggtatg gaaatcaaaa aagatacgat tgttttttgt atgatcggtg cggctaaccg
    11701 ggaccctgaa gcatttgaac agcctgacgt gtttaatatt catcgggaag atcttggcat
    11761 caagagcgct tttagcggcg ccgcccggca tctcgctttc ggatccggca ttcataactg
    11821 tgtaggagca gcttttgcca aaaccgaaat cgaaattgta gctaatattg tgctggataa
    11881 gatgcggaat atcagattag aggaagattt ttgttatgct gagtccggtc tgtatacacg
    11941 cggacccgtc tcacttcata ttgtatttga cagggcttaa taatatggaa aggtctctcc
    12001 cgtgcgggtg aggctttttt acatatgctt gatccacaag ttaataaacg cagtcactgt
    12061 tttcaactca tcaattgaaa atttattgaa gagctgtttg tattgctcct ttgctttttg
    12121 atgaagccgt tcatgtaacc ccgccagttc tttgccgctt tcagttaacg tataaaaaat
    12181 ttcttttttg ttgcccggct tttttgtcac tgtaatgatg ttatgtttca acaaagcttg
    12241 aattgctttt gaaacagctg cttttgagat atgtaagcgg gatgctaaga agctgttatt
    12301 cacatttttg ctggtatgta aacaggaaac aatgtgaagc tgggtcaatg tccagtcttt
    12361 tttgaggtgg tcgagatgtt gtgaggacgg aatctgcacc ttttgccaga cgcgtttatc
    12421 tctttttaat gcgaattgtt gtatagcttg aaataattca tcatgaatgc tgttgttcat
    12481 caatttgcct ctctgattta attgttaact ggttaactat ccttagttta ctggggaata
    12541 gtggaatttt caagaggcag acgttttttt gttctttatg cacaaaaatg aactcataag
    12601 cttccctgtt cagacgcaaa atacatgaaa agggaggtgc tttgatgagg aaatacacgg
    12661 ttattgcttc tatattactg tcttttcttt ccgttttgtc tggcggccat catgaatcaa
    12721 aagcttttcc tgttgtacag caggaactat ttcaaacgcc tcattacata cccttgcttg
    12781 aaaatcctcc tcaaattgat gtgaaagctg aaatgagaaa caatctaatg attgaggctc
    12841 aagttcgcga taattcgtat caagcatttt ccgcctttct tttttataaa caaagtaatg
    12901 agctgggcta caaaatggtg ccgatggacc cgacccccgg agcggtccgc aagtttttgg
    12961 cacaaattcc gaaacgtttg atttggaaca gcgagcttga gtattacatt gtgatgtcga
    13021 atgggaagca gcgggtggag tcggagatga agaaactgaa gcttgaagga taccaggctg
    13081 accttgccca tattccggag cttctgatca cagagctggc ggttgatacg aagaatatag
    13141 gacgtgcaga cggttatgaa tttattgaga tttttaatac gactgacaga acgattgatt
    13201 ttaaagacta ccatatccgt taccgctacc caaaagaagg gcctgactcc gatctgattt
    13261 ggagacctga tgaaaggatc ttgataccat ccggcgaaac gtttgtcgtc tggctgaagc
    13321 ctgccgggca tccagaattg acgtcagagg atttcaatcg ttattatcag acacaactta
    13381 aagaaggaaa aaatctcgcg gtgatagacg agacagaggg tatggccaat acgaggccgc
    13441 gggccgttgt gatatcaacg aatacaggaa aggacatttc ggttgcgcat tatcggaaac
    13501 atgcgctgcg ccggctgtca tcagtgcttt ataagtatcc gttgaacgga accgcagaac
    13561 ttctgaacat ttcaatcggg gaaaagaatc catctcccgg aaccgtatta caggcacagg
    13621 tgcccgagca gaaacgaaaa atcaagctcg ataaagaaaa gccggtcatt gaggatttga
    13681 ctgaccgcaa gcctgttcgc cctgctgaaa gcattgagct gcgggctgac atacgggacc
    13741 gaagtctagt gaaaacagcg gccttttact accggacgga tgagaacaaa ccgtttaagc
    13801 gaattcttgc tgaaaaagac cgcaacgata atctctacca ttatattgtg tattcgcctg
    13861 aattgatcgg gaaggatcag ctagagtatt atgtggcggc cggggacggg atcaatgaag
    13921 cgagaacgcc ggtcaaaatg attgatatta aacaaacaag caagtcgcac ggtctgcgga
    13981 tgaatattga aaacagagac acgctgtcgg gaactcaatt tctcaaagcc acgacagaag
    14041 ggcgagctga ccgcattaag ctatggattg acgggaaaaa acaagtgaca gagcccgcga
    14101 tggaaaaaga agtatatttt gcattcgata cgaggaaaac gaacctttat tttaaaaacg
    14161 cggtaacgat ggaaggcaaa gtgctgaagg tgttcgatga tacaacaaat aaataccgca
    14221 cctattctgt tccattgcca gagacactgc ttcggaaagg gaagcagctg cagcgtatta
    14281 caatacgatc gggttcgaag gtgtctccgt ttgatacggc tgaaaaccgt gatgacttct
    14341 tagtcaaaaa cgcccgattg gtgttatcgg atggaacggt gatcagggat cagagggtgt
    14401 cccctgagaa ggaactcttt attggcgata atcagcgctc gaataaaagc tggcaatttc
    14461 agttcaatct gcctgacgga ctgtttacat cacagctgct ggagtgggac acatcaaagc
    14521 tttctgaagg cgctcaccat attcaagcaa gcgacggcaa ggaaaatgtc agcctggttg
    14581 tacgggtaga taattcagga cctcacattg agccgaatat caccgagggg caaacgtata
    14641 aaggaaacct tatcctgcag gcggatatgt atgataaatg gagcaaaatt gaggaagcgg
    14701 aagcgtcttt agatggagaa agcattacac tcccatatca tacgtcctct tctgatcttt
    14761 tgccgggaaa acattctcta aaagtgacag caacggatct cgcgggcaat aaaacagtga
    14821 ttgagaggat atttaaaacc gaacgagagc atccggacca gccggaggtt atagacagcg
    14881 aagccgacac gcataaggcc aagctttctg tccgggtgaa agatccgacg aatgatgcca
    14941 tggatgttgg tttttatagg ggatttcaat atacagcgcg aaatcatgtg aaaatattta
    15001 agcacgcgtc actgactgaa ccgccgaaaa gctttgtgcc ggaaagtgag accccattta
    15061 cgaataaaga attggagcgg gtatctgctg ctgatggaaa aacagtatcg acagaaaaca
    15121 aagagctttt cccttatcat cgctttgaag tgacggttga tccatcaatt gatgagaatg
    15181 atctggcaga agcagtctgg aagggcagtt ctttgccggg gagaaaggtg acgatgtatg
    15241 cttggaatta tcggacaaac gaatggcagc ctgttgacag cttcgtcgcg aaggatgaca
    15301 aaccgtttac gctgaaagcg tctgtgattg ccgccgattt tgtacgggag tcgaaaatga
    15361 atgtgattgt acaggatgaa attcctcctg cgaaggatat gtacacattt gtctggatgt
    15421 ctgatacaca gtattacgcc gaaagctatc cgcatatttt tgataaacag accgaatgga
    15481 taaaagataa tcaaaagcag ctcaatatca aatatgtgtt ccacacgggt gatattgtag
    15541 atgattccgc cgatatcaga cagtggaaaa acgctgatcg gtcaatgtcc gttctggata
    15601 agagcggaat accttacggc gtgctggccg gcaaccacga tgtcgggcat aaggacggct
    15661 cctacagggt gtttggcaaa tattttggca gcgatcgttt tgataaaaaa ttgcattatg
    15721 gcggttccta taaaaataac cgcggacatt atgacctgat ttcgagcaac ggcaatgatt
    15781 atatcatgct ttacatggga tggggaataa cggatcagga cattgcgtgg atggatcagg
    15841 tgctgaagaa gcatcctgac cggatggcca ttttagcttt tcatgagtat ttgctggtca
    15901 gcggaaacag aagtccgatc ggagagaaaa tattcaaaga aatcgtaaag ccgaacccga
    15961 atgtcgtgat ggtgttgagc gggcattatc atagtgcgat gagaaaaaca gatgagctgg
    16021 atgatgatgg tgatgggaag ccggatcgtc ttgtgcatca aatgctggcc gattatcagg
    16081 gcggaccaga aggcggtcaa gggtatttgc gtttgcttca atttgaccag gccaatgaca
    16141 tggtacacgt atcgacgtat tctccgtatg taaaggataa gaattactat gatacggata
    16201 cctatggcaa caaggatgaa ttttcccttt cgcttgattt gaagccaaga atcaaaaagg
    16261 tggaaaccga ttattttgaa tgtaacgtat ataccaatga agagctcgga aaacgggagc
    16321 aagtgaagag cggggataca gctgaattcc gctgggatca tcttgaaccg cagtctgttt
    16381 attattggta cattatagtg gaagacagtt ttaacggcaa aacgaaatca ccgatctgga
    16441 agttcaagac gaaaaaagaa acatatcgtc cggcaccaga ccagtttgat tttcgccatg
    16501 tctccaatcc ttaaaaaggg ttggaggctt tttttatgaa agacgatcca tatagtggac
    16561 aaatgtataa cggtcgcctc tgtatacgga ttttgcatgc tcaaaagcag ttccgttctg
    16621 cagatatgtc gttcgtttaa ttaataggac aggcgctccc ttttgaatac caagaatatt
    16681 cgcttcttcc atggtggcag cgcttggctc aagctcctgt tttgcacggg aaatcggtat
    16741 gctgttatac ctttcaatat gatcatagat cgacgactga aaatgcgatt cgttcaattc
    16801 acccgcaagc tcaaacggaa tatgtgagga ctcaatcgcc attggaatat catttgccag
    16861 ccgcacccga gtgattttat ggatagagga ggggtgcccg cagcctaata tagccgcgag
    16921 ctcctcagtt gaatcaataa gctgataatc aatgagcctg ctgcccggtg tcatcccgcg
    16981 gcttttcata tcctcggtaa aacttgtcag cccttgaagt gcttgttcca tttttggctt
    17041 gctgacaaag gtgccccgcc ccttcaggcg atagagcaag ccttcattaa ctaaattaga
    17101 aagcgcctgg cgaactgtca tccggctgat cccgaattgt tcggcatatt cgcgctcaga
    17161 aggaagaggc atatccggct gcagttctcc gctcttaatt tgggttttta attgctccat
    17221 aatctgataa taaatcggaa taggcgattg tttattgata ttcataagtc agcatgttcc
    17281 tttcaaggtc ttaatgacgc ggcttcacga tcaatcaaaa ccgtgacatc ggaatgcagg
    17341 tgcaaagcag atgcgggaaa atcttcgctt atgtttcctt ctaacagctt acgaacagct
    17401 tctgctttgc ttttcccaga aataagcagc agaatgcgtt tgcttgagag aatcgttcga
    17461 attcccattg tcagtgcttt tttcggcacg ctgtctatgg aaggaaaata tctggcattc
    17521 gcttggcggg tttgctcatt taatgtcaca acatgagttc gcgacttgaa agaggttccc
    17581 ggttcgttaa agccgatgtg tccgtttcgg ccgatgccga gaagctgaat gtcagtgccg
    17641 ccgagggact cgactagctg ttcataccgt ctgcattcgg cttccaaatc gtctgcattt
    17701 ccattcggaa taaaatgccg gctaggcttg ctgtcgatac gctgaaataa acggtcattc
    17761 atatagaagt gatagctgtt cgggtcattg cttgaaagtc tggcgtactc atccaaatta
    17821 actgtagtga cgttttgaaa tgagagattc tcagtttggt gcaggcggat cagctcgcga
    17881 taagtgcctt ccggtgtgcc gcccgtcgct aacccgagaa cggcatcagg tttttctttg
    17941 attgtatctg ccgttattct ggctgctttt tggcttagct cttcatacgt ttgacattcc
    18001 attactttca tatctggtca gcctccttgg atataaatgc aatgtttccg cggcaaatgg
    18061 tgagaatcac ctcgcaatca ctgctgacaa tcaccaaatc tgcatctttt cccacagtca
    18121 cactgccttt tcggtcaaag atgcccaact gtttggccgc gtttgcagaa gttatattag
    18181 ctatatccgt ccaagagcaa tttgtaaact cacgcatatg ccgtgcgccc tcgttcattt
    18241 tgaggatcga accggcaagg gttccgtctg aaagaagagc tgttcgtcct cttaccgtca
    18301 cactttggcc gccgaactca tatacgccgt ctttcagtcc ttttgccctc atggaatcgg
    18361 taattaaaat gagcttgctg ctgcctttcg ccaaaaaagc aagcttagcc gctaaaggat
    18421 gggagtggat gccatcggct attagctctg tcacaaaccc atcatgggcc agcgccgttc
    18481 cgatcactcc gggttctcgg tgatgaaacg ggctcatcgc gttgtacaga tgggtcatat
    18541 ggctcgcccc tgcttttgcg gcatccgaca gcattgcgga atcggcatcc gtgtgcccca
    18601 tggacgcgat aatcgattct tctttcaaat ggcggatcag ttcaaaatgc tgatcctcct
    18661 ccggcgcaag tgtaacaatt ttgatcagtc cgcccgcctc ctgctgccac tttttgaaaa
    18721 gcgcaacatc cgaaggccta atccactctt ttggctgcgc tccggctctt ttaggtgaaa
    18781 caaaggggcc ctctaaatga atgccgagca gttctgcacc taataaagag gactcttcag
    18841 ccgctttcca ctctcttgcg ttcaccaaag cttgagaaat gttcccatgc tcttgggtaa
    18901 tcgttgtggc taaaaagctc gtcgtgcctt cttcgggaag tcttgacgac atgatgtcga
    18961 gcgcagaaaa gcttgcatcc atcgtatctg cgccatagcc gccgtggata tgaatgtcaa
    19021 tcatgccggg aagcaaaacg gaatccgcag gtgcttgaat ttcttttgaa tatgactcct
    19081 tgggcagttc tgttgagact gtgctgattt ttccgtcatt gattccgaca tagccgtttt
    19141 tgattacttc attttctgtc acgatcgcaa tgtctttgat aagaagactc tctgccatag
    19201 atgatccgcc tttctggaat ttgaatacct ttattttact ctttttcaga ctagtgatct
    19261 agaccagctg aggtgttttc ccgcctttta taaaatctgt tttaggttca agcctgaaag
    19321 tgataaaatg aaagtattgt gagcaagctc ggggcagccc ggaacaggag gaacatatcg
    19381 tggcaaaggt tcgcacaaaa gacgtaatgg aacagttcaa tttggaatta atcagcggag
    19441 aagaaggaat taaccgcccg attacaatga gtgacctatc aagaccaggc attgaaattg
    19501 ccggatattt tacatattac ccgagagagc gcgtgcagct tctcggaaaa acagagcttt
    19561 ctttctttga acagcttcct gaagaggaga aaaaacagcg aatggattct ctgtgcactg
    19621 atgtaacacc agctattatt ctttctagag atatgcccat cccgcaggag ctgattgacg
    19681 cctccgagaa aaatggggtg cctgtcctca gatcgccgct gaaaacgaca agactgtcaa
    19741 gccgtttaac caacttcctt gagagcaggc tggcgcctac cacagcaatt catggcgttc
    19801 ttgtcgatat atatggtgtc ggcgtgctga tcacaggaaa gagcggcgtc ggaaaaagcg
    19861 aaacagcgct ggagcttgtg aaaagagggc atcgccttgt tgccgatgat tgcgttgaaa
    19921 ttcggcagga ggatcaggat acccttgtcg gaaacgctcc ggaattaatt gagcatctcc
    19981 ttgaaatcag ggggcttggc attatcaacg tgatgacgct gtttggcgcc ggcgcggtca
    20041 gaagcaataa acgaatcacg attgtcatga atcttgaact ttgggagcag ggcaagcaat
    20101 acgaccggct cggacttgag gaagagacca tgaaaatcat tgatacagaa atcacaaaac
    20161 tgacgattcc cgtccgtcca ggccgaaatc ttgcagttat catcgaagtg gcagcaatga
    20221 acttcagatt gaagcggatg gggctgaatg ccgctgaaca attcaccaac aagctggcgg
    20281 acgtcattga agacggtgaa caagaagaat aggagttggc gttaaatgaa tgaagcgata
    20341 gaaccactca atccgatagc atttcagctg gggccgttag ccgtccattg gtacggaatc
    20401 attatcggtc tcggcgcttt gctcgggctg tggattgcga tgagggaaag tgaaaaacgg
    20461 gggctgcaga aagatacgtt tattgatctt gtcctgtttg ccattccgat tgcgattatt
    20521 tgcgcgcgaa tttattacgt tgcctttgaa tgggactatt atgcggcaca tccgggagaa
    20581 atcattaaga tttggaaggg cggcattgcc attcatggcg gattaatcgg cgccatttta
    20641 acgggatatg tgttttcgag agtgaaaaac ctttcgttct ggaagcttgc agatattgcc
    20701 gcgcccagca ttttgctcgg ccaggcgatc ggccgctggg ggaactt
//
