LOCUS       NZ_QRFZ01000006        10488 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis subsp. subtilis strain EA-CB0575 BS575_6, whole
            genome shotgun sequence.
ACCESSION   NZ_QRFZ01000006
VERSION     NZ_QRFZ01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis subsp. subtilis
  ORGANISM  Bacillus subtilis subsp. subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Franco-Sierra,N.D., Posada,L.F., Santa-Maria,G., Romero-Tabarez,M.,
            Villegas-Escobar,V. and Alvarez,J.C.
  TITLE     Bacillus subtilis EA-CB0575 genome reveals clues for plant growth
            promotion and potential for sustainable agriculture
  JOURNAL   Funct Integr Genomics 20 (4), 575-589 (2020)
   PUBMED   32198678
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Franco-Sierra,N.D., Santa-Maria,G. and Alvarez,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2018) Process Engineering, Universidad EAFIT, Cra
            49 7 sur 50, Medellin, Antioquia 050022, Colombia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAR-2015
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011800745.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,261
            CDSs (total)                      :: 4,164
            Genes (coding)                    :: 4,041
            CDSs (with protein)               :: 4,041
            Genes (RNA)                       :: 97
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 62 of 123
            Pseudo Genes (incomplete)         :: 65 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 21 of 123
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:02:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10488
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QRFZ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1319
                     /locus_tag="DXT72_RS01795"
     source          1..10488
                     /altitude="28 m"
                     /collected_by="Cenibanano"
                     /collection_date="2009"
                     /db_xref="taxon:135461"
                     /geo_loc_name="Colombia: Uraba"
                     /host="Musa acuminata AAA Group"
                     /identified_by="Maria Ramirez-Correa"
                     /isolation_source="rhizosphere"
                     /lat_lon="7.866533 N 76.638562 W"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis subsp. subtilis"
                     /strain="EA-CB0575"
                     /submitter_seqid="BS575_6"
     region          1..10488
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10488
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10488
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1400:>10488](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1401..>10488
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        <7..830
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS01795_Epimerization.1"
                     /evalue="6.10E-156"
                     /label="DXT72_RS01795_Epimerization.1"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="275"
                     /protein_start="0"
                     /score="512.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="MLHAKKGFDPERVEKTLQALIEHHDALRMVYREGQEDVIQYNRGL
                     EAASAQLEVIQIEGQAADYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAI
                     HHLVVDGVSWRILLEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERLQDFANSKAFLKEK
                     EYWRQLEEQAVAAKLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDI
                     LLTAFGLAMKEWTGQDRVNVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDM"
     aSModule        <7..1307
                     /domains="nrpspksdomains_DXT72_RS01795_Epimerization.1"
                     /domains="nrpspksdomains_DXT72_RS01795_TIGR01720.1"
                     /incomplete
                     /locus_tags="DXT72_RS01795"
                     /tool="antismash"
                     /type="unknown"
     CDS             <5..1319
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-275). E-value:
                     6.1e-156. Score: 512.3. Matches aSDomain:
                     nrpspksdomains_DXT72_RS01795_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (280-434). E-value: 3.3e-56.
                     Score: 181.4. Matches aSDomain:
                     nrpspksdomains_DXT72_RS01795_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="DXT72_RS01795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_167407844.1"
                     /sec_met_domain="Condensation (E-value: 2.7e-87, bitscore:
                     281.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLHAKKGFDPERVEKTLQALIEHHDALRMVYREGQEDVIQYNRGL
                     EAASAQLEVIQIEGQAADYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAI
                     HHLVVDGVSWRILLEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERLQDFANSKAFLKEK
                     EYWRQLEEQAVAAKLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDI
                     LLTAFGLAMKEWTGQDRVNVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDMKHADDL
                     GYQLKQMKEDIRHVPNKGVGYGILRYLTASEHKEDVAFSIQPDVSFNYLGQFDEMSDAG
                     LFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLEFHEKTVQTFSDSFKA
                     HLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKLMEIF"
     CDS_motif       27..101
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS01795_0001"
                     /evalue="1.50E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="32"
                     /protein_start="7"
                     /score="20.6"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       303..368
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS01795_0002"
                     /evalue="1.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="121"
                     /protein_start="99"
                     /score="39.7"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       777..824
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS01795_0003"
                     /evalue="7.40E-09"
                     /label="NRPS-E5"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="273"
                     /protein_start="257"
                     /score="27.6"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     aSDomain        846..1307
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS01795_TIGR01720.1"
                     /evalue="3.30E-56"
                     /label="DXT72_RS01795_TIGR01720.1"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="434"
                     /protein_start="280"
                     /score="181.4"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTASEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     CDS_motif       870..929
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS01795_0004"
                     /evalue="7.20E-10"
                     /label="NRPS-E6"
                     /locus_tag="DXT72_RS01795"
                     /protein_end="308"
                     /protein_start="288"
                     /score="31.0"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        1401..2246
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21235_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="6.70E-104"
                     /label="DXT72_RS21235_Condensation_DCL.1"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="282"
                     /protein_start="0"
                     /score="339.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQDVQRQGFNLAKDILFKVA
                     VFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEYSFTWDETLVARIQQTAN
                     LHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     V"
     aSModule        1401..4367
                     /complete
                     /domains="nrpspksdomains_DXT72_RS21235_Condensation_DCL.1"
                     /domains="nrpspksdomains_DXT72_RS21235_AMP-binding.1"
                     /domains="nrpspksdomains_DXT72_RS21235_PCP.1"
                     /locus_tags="DXT72_RS21235"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            1401..>4373
                     /gene="srfAB"
                     /locus_tag="DXT72_RS21235"
                     /pseudo=""
     CDS             1401..>4373
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (0-282). E-value:
                     6.7e-104. Score: 339.1. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21235_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (447-840). E-value: 2e-111.
                     Score: 364.4. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21235_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (946-989). E-value: 4.2e-14. Score:
                     44.5. Matches aSDomain: nrpspksdomains_DXT72_RS21235_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="DXT72_RS21235"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.4e-83, bitscore:
                     268.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.5e-117, bitscore:
                     379.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-09, bitscore:
                     26.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQDVQRQGFNLAKDILFKVA
                     VFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEYSFTWDETLVARIQQTAN
                     LHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     VERDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYPLDQELE
                     NGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWTVKIKYNGAAFDSAFIERTAEHLT
                     RMMEAAVAQPAAFVREYGLVGNEEQRQIVEVFNSTKAELPEGMAVHQVFEEQAKRTPAS
                     TAVVYEGTVLTYRELNAAANRLARKLVEQGLQKGETAAIMNDRSVETVVGMLAVLKAGA
                     AYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTGQAHQLQVPVLTLDIGIEDGEADNLN
                     LPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLVKNAGYVPVTEEDRMAQTGAVSFDA
                     GTFEVFGALLNGAALYPVKKETLLDAKQFAAFLREQSITTMWLTSPLFNQLAAKDAGMF
                     GTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYGPTENTTFSTSFLIDREYGGSIPIG
                     KPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGVARGYVNLPELTEKQFLEDPFRPGERI
                     YRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELGEIETKLNMAEHVTEAAVIIRKNKA
                     DENEICAYFTADREVAVGELRKTLSQSLPDYMVPAHLIQMDSLPLTPNGKINKKELPAP
                     QSEAVQPEYAAPETESEKKLAEIWEGILGVRAGVTDNFFRIGGHSLKAMMMTAKIQEHF
                     HK"
     CDS_motif       1422..1538
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0001"
                     /evalue="2.70E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="46"
                     /protein_start="7"
                     /score="45.4"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     CDS_motif       1755..1820
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0002"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="140"
                     /protein_start="118"
                     /score="37.1"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       1866..1904
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0003"
                     /evalue="1.10E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="168"
                     /protein_start="155"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       2142..2237
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0004"
                     /evalue="7.10E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="279"
                     /protein_start="247"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       2286..2402
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0005"
                     /evalue="1.60E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="334"
                     /protein_start="295"
                     /score="55.9"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        2742..3920
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21235_AMP-binding.1"
                     /evalue="2.00E-111"
                     /label="DXT72_RS21235_AMP-binding.1"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="840"
                     /protein_start="447"
                     /score="364.4"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTVLTYRELNAAANRLARKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTG
                     QAHQLQVPVLTLDIGIEDGEADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRL
                     VKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLR
                     EQSITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGY
                     GPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGVA
                     RGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       2928..2969
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0006"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="523"
                     /protein_start="509"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       3153..3212
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0007"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="604"
                     /protein_start="584"
                     /score="35.7"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       3714..3803
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0008"
                     /evalue="2.60E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="801"
                     /protein_start="771"
                     /score="48.5"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       3885..3950
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21235_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="850"
                     /protein_start="828"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4239..4367
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21235_PCP.1"
                     /evalue="4.20E-14"
                     /label="DXT72_RS21235_PCP.1"
                     /locus_tag="DXT72_RS21235"
                     /protein_end="989"
                     /protein_start="946"
                     /score="44.5"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVRAGVTDNFFRIGGHSLKAMMMTAKIQEHF"
     gene            4465..4605
                     /gene="comS"
                     /locus_tag="DXT72_RS21240"
     CDS             4465..4605
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="DXT72_RS21240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            <4551..>7493
                     /gene="srfAB"
                     /locus_tag="DXT72_RS21245"
                     /pseudo=""
     CDS             4551..>7493
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     5.3e-94. Score: 306.5. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21245_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (427-830). E-value:
                     3.7e-115. Score: 376.7. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21245_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (936-980). E-value: 2.3e-17. Score:
                     54.9. Matches aSDomain: nrpspksdomains_DXT72_RS21245_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="DXT72_RS21245"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.9e-85, bitscore:
                     274.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2e-126, bitscore:
                     410.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-10, bitscore:
                     30.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAANPNVRLSDV
                     DLLSSAETAALLEERHMTQITEATFAALFEKQAQQTPDHSAVKAGGNLLTYRELDEQAN
                     QLAHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLED
                     SGAKFAVVNERNMTAIGQYEGIIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTG
                     KPKGVQIEHRNLTNYVSWFSEEASLTENDKTALLSSYAFDLGYTSMFPVLLGGGELHIV
                     QKETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKII
                     PTDVIAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALV
                     LNEALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRL
                     SDGTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTS
                     DQAIEKAELRYQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALA
                     APETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE"
     aSDomain        4557..5342
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21245_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.30E-94"
                     /label="DXT72_RS21245_Condensation_LCL.1"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="264"
                     /protein_start="2"
                     /score="306.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     aSModule        4557..7490
                     /complete
                     /domains="nrpspksdomains_DXT72_RS21245_Condensation_LCL.1"
                     /domains="nrpspksdomains_DXT72_RS21245_AMP-binding.1"
                     /domains="nrpspksdomains_DXT72_RS21245_PCP.1"
                     /locus_tags="DXT72_RS21245"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4560..4673
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0001"
                     /evalue="1.60E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="41"
                     /protein_start="3"
                     /score="55.8"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     CDS_motif       4869..4934
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0002"
                     /evalue="8.20E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="128"
                     /protein_start="106"
                     /score="28.0"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       4965..5000
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0003"
                     /evalue="1.20E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="150"
                     /protein_start="138"
                     /score="11.6"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       5247..5336
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0004"
                     /evalue="9.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="262"
                     /protein_start="232"
                     /score="50.2"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       5391..5504
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0005"
                     /evalue="2.40E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="318"
                     /protein_start="280"
                     /score="52.1"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        5832..7040
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21245_AMP-binding.1"
                     /evalue="3.70E-115"
                     /label="DXT72_RS21245_AMP-binding.1"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="830"
                     /protein_start="427"
                     /score="376.7"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHSAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKFAVVNERNMT
                     AIGQYEGIIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTALLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPTDVIAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       6018..6059
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0006"
                     /evalue="3.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="503"
                     /protein_start="489"
                     /score="17.1"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       6246..6305
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0007"
                     /evalue="9.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="585"
                     /protein_start="565"
                     /score="33.5"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       6834..6923
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0008"
                     /evalue="4.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="791"
                     /protein_start="761"
                     /score="51.0"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       7005..7070
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21245_0009"
                     /evalue="3.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="840"
                     /protein_start="818"
                     /score="38.3"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        7359..7490
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21245_PCP.1"
                     /evalue="2.30E-17"
                     /label="DXT72_RS21245_PCP.1"
                     /locus_tag="DXT72_RS21245"
                     /protein_end="980"
                     /protein_start="936"
                     /score="54.9"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAEL"
     aSDomain        7644..8471
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21250_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.40E-100"
                     /label="DXT72_RS21250_Condensation_LCL.1"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="276"
                     /protein_start="0"
                     /score="326.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="MYVLNQLDRRTISYNMPSVLLMEGELDISRLRDSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLNDAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPTLHYKDFAVWQNEAEQKER
                     MKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTLALRMEA"
     aSModule        7644..10130
                     /domains="nrpspksdomains_DXT72_RS21250_Condensation_LCL.1"
                     /domains="nrpspksdomains_DXT72_RS21250_AMP-binding.1"
                     /incomplete
                     /locus_tags="DXT72_RS21250"
                     /tool="antismash"
                     /type="nrps"
     gene            7644..>10488
                     /gene="srfAB"
                     /locus_tag="DXT72_RS21250"
                     /pseudo=""
     CDS             7644..>10488
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-276). E-value:
                     3.4e-100. Score: 326.8. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21250_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (441-829). E-value:
                     4.7e-106. Score: 346.7. Matches aSDomain:
                     nrpspksdomains_DXT72_RS21250_AMP-binding.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="DXT72_RS21250"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.3e-93, bitscore:
                     301.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-114, bitscore:
                     371.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYVLNQLDRRTISYNMPSVLLMEGELDISRLRDSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLNDAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPTLHYKDFAVWQNEAEQKER
                     MKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTLALRMEAKEQQT
                     FAELLEQAKQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMVTTENPDKEQLTLQ
                     NLSISPYEAHQGTSKFDLTLGGFTDENGIGLQLEYATDLFAKETVEKWSEYVLRLLKAV
                     ADNPNQPLSSLSLVTETEKQALLEAWKGKALPVPTDKTVHQLFEETVQRHKDRPAVTYN
                     GQSWTYGELNAKANRLARILMDCGISPDDRVGILTKPSLEMSAAVLGVLKAGAAFVPID
                     PDYPDQRIEYILQDSGAKLLLKQEGISVPDSFTGDVILLDGSRTILSLPLDENDEGNPE
                     TAVTAENLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFSMTAEDRSAKYAGFGFD
                     ASIWEMFPTWTIGAELHVIDEAIRLDIVRLNDYFETNGVTITFLPTQLAEQFMELENTS
                     LRVLLTGGDKLKRAVKKPYTLVNNYGPTENTVVATSAEIHPEEGSLSIGRAIANTRVYI
                     LGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTGDLVKWT
                     GGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAVTAVKDKGGNTAIAAYVTP
                     ESADIEALKSALKETLPDYMIPAFWVTLNELPVTANGKVDRKALPEPDIEAGSGEYKAP
                     TTDMEELLAGIWQDVLGM"
     CDS_motif       7680..7790
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0001"
                     /evalue="5.80E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="49"
                     /protein_start="12"
                     /score="54.1"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRDSLNQLVNRHESLRTSF"
     CDS_motif       7986..8051
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0002"
                     /evalue="1.70E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="136"
                     /protein_start="114"
                     /score="30.2"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       8082..8114
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0003"
                     /evalue="1.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="157"
                     /protein_start="146"
                     /score="14.8"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8373..8462
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0004"
                     /evalue="1.00E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="273"
                     /protein_start="243"
                     /score="46.8"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       8511..8624
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0005"
                     /evalue="5.30E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="327"
                     /protein_start="289"
                     /score="57.4"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        8967..10130
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXT72_RS21250_AMP-binding.1"
                     /evalue="4.70E-106"
                     /label="DXT72_RS21250_AMP-binding.1"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="829"
                     /protein_start="441"
                     /score="346.7"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FEETVQRHKDRPAVTYNGQSWTYGELNAKANRLARILMDCGISPD
                     DRVGILTKPSLEMSAAVLGVLKAGAAFVPIDPDYPDQRIEYILQDSGAKLLLKQEGISV
                     PDSFTGDVILLDGSRTILSLPLDENDEGNPETAVTAENLAYMIYTSGTTGQPKGVMVEH
                     HALVNLCFWHHDAFSMTAEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIV
                     RLNDYFETNGVTITFLPTQLAEQFMELENTSLRVLLTGGDKLKRAVKKPYTLVNNYGPT
                     ENTVVATSAEIHPEEGSLSIGRAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYL
                     NREDETAKRFVADPFVPGERMYRTGDLVKWTGGGIEYIGRIDQQVKVR"
     CDS_motif       9153..9194
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0006"
                     /evalue="9.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="517"
                     /protein_start="503"
                     /score="18.8"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       9396..9455
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0007"
                     /evalue="6.90E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="604"
                     /protein_start="584"
                     /score="33.9"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
     CDS_motif       9927..10016
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0008"
                     /evalue="3.90E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="791"
                     /protein_start="761"
                     /score="51.1"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNREDETAKRF"
     CDS_motif       10095..10160
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXT72_RS21250_0009"
                     /evalue="2.00E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="DXT72_RS21250"
                     /protein_end="839"
                     /protein_start="817"
                     /score="35.9"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
ORIGIN
        1 tgatgcttca cgccaaaaag ggctttgatc ctgaacgggt ggagaaaaca ttgcaggcgc
       61 tgatcgagca tcatgacgcg ctccgtatgg tctaccgtga gggacaggaa gacgtcattc
      121 aatacaacag aggtcttgaa gctgcttccg ctcaattgga ggtcatccag attgagggcc
      181 aagctgcaga ttacgaagac cgtatagaga gagaagcgga gcgtttgcaa agcagcatcg
      241 acttgcagga aggcggcttg ttaaaagcag gcttgttcca agcggaagac ggagaccact
      301 tgcttctcgc cattcaccac ttagtggttg acggcgtctc gtggcggatt ctactggagg
      361 atttcgccgc agtatataca cagcttgagc aaggcaatga accggttctc ccgcagaaaa
      421 cacattcatt tgcagagtat gcagagagat tgcaagactt cgcgaactcc aaagcctttt
      481 tgaaagaaaa agagtattgg agacagcttg aagaacaagc tgtcgcggca aagcttccga
      541 aagaccgcga atctggtgat cagcgaatga aacatacaaa gacaattgaa ttctcgctga
      601 ctgctgaaga gacagaacag ctcaccacaa aggtgcatga ggcatatcac acagaaatga
      661 atgatatttt gctgacggca ttcggattgg caatgaagga gtggacgggt caggatcgag
      721 taaatgttca tttagagggg cacggacgtg aagaaatcat agaagacctg accatttccc
      781 gcacagtcgg ctggtttaca agtatgtacc caatggtgct cgatatgaag catgcggatg
      841 atctgggcta ccagctgaag caaatgaaag aagatatcag acatgtgccg aataagggag
      901 tcggatacgg cattttgcgc tatctgacgg catcggaaca taaagaagat gtggcgttct
      961 cgattcagcc ggatgtcagc ttcaactatt taggtcagtt tgacgaaatg tcggatgcag
     1021 gtttgtttac gagatcagag ctgccatcag gacagtcatt aagccctgaa acagaaaaac
     1081 cgaatgcgct ggatgttgtc ggatatatcg aaaacggaaa actgacgatg tcactggcct
     1141 atcattctct tgaatttcat gaaaaaacag tacaaacatt cagtgacagc tttaaagcgc
     1201 atcttctcag aatcattgaa cattgcctat ctcaagacgg tacggaactg accccgagtg
     1261 atcttggtga cgacgatttg acgctggatg agctggataa attaatggaa attttctaat
     1321 agaggtggca tatgagcaaa aaatcgattc aaaaggtgta cgcactgaca ccaatgcagg
     1381 agggaatgct gtatcatgcg atgcttgatc cgcattcttc ctcgtacttc acacaattag
     1441 agcttgggat tcacggcgct tttgatcttg aaatctttga gaaaagcgtc aatgaactga
     1501 ttcggtcata cgatattctc cgtacggtat ttgtgcatca gcagctgcaa aaaccgcgtc
     1561 aggtcgtgtt agcggaacgc aaaacaaagg tgcattatga ggatatcagt catgcagacg
     1621 agaatcgcca gaaggagcac attgagcgtt acaaacaaga cgttcagcgc caaggcttta
     1681 acctggcaaa agacatattg ttcaaggtgg cggttttccg ccttgctgca gatcagctgt
     1741 atcttgtctg gagcaatcat catattatga tggacggctg gagcatgggc gtcctcatga
     1801 aaagcctgtt ccaaaactat gaagcgctca gagcaggcag gacaccggca aacggtcagg
     1861 gcaagcctta ttccgattac atcaagtggc ttggaaaaca ggacaatgaa gaagcggaga
     1921 gctactggag cgagcgcttg gcgggttttg aacagccaag cgtgctcccg ggccgcctgc
     1981 ctgtaaaaaa agacgaatac gtcaataaag aatattcatt tacatgggac gaaacactgg
     2041 ttgcccgtat tcaacaaacc gcaaatctcc atcaagtgac agggcctaac ctatttcagg
     2101 ccgtttgggg cattgttctc agcaaataca actttacgga tgatgtggtc ttcggaacgg
     2161 tcgtctcggg acgaccgtct gaaatcaacg gaatcgaaac gatggcgggg ctgtttatca
     2221 acaccattcc agtgcgggtg aaagttgaac gagatgctgc attcgctgat attttcacag
     2281 ctgttcagca gcatgcagta gaggcagagc gttacgatta cgtgccgctc tatgagattc
     2341 aaaaacgctc agctcttgat ggcaatctct taaaccatct tgtcgcgttt gaaaattatc
     2401 cgcttgatca agagcttgaa aacggcagca tggaagaccg cctcgggttt tcgattaagg
     2461 tagaaagcgc atttgaacaa acgagcttcg atttcaacct gattgtgtat ccgggcaaaa
     2521 cgtggaccgt caaaattaaa tataacggag ccgcctttga ttccgctttt atcgaacgga
     2581 cagcggagca ccttacccgc atgatggaag cagctgtcgc tcagccggcc gcttttgtgc
     2641 gtgagtacgg gcttgttgga aacgaagagc agcggcaaat tgtcgaggtg tttaacagca
     2701 cgaaagccga actccctgaa ggaatggctg tacaccaagt gtttgaagag caagcgaaac
     2761 ggacgccggc gagcactgcc gtcgtatatg aaggaaccgt gctgacgtac cgcgagctga
     2821 atgcagcggc taaccgtctg gcgagaaagc ttgtcgaaca aggccttcaa aaaggggaaa
     2881 cagcagcgat tatgaacgat cgatcagtag aaaccgttgt cggcatgctg gctgtgttaa
     2941 aagcaggcgc cgcatatgtg ccgcttgatc cagcgcttcc gggggatcgt cttcgtttca
     3001 tggcagagga cagctccgtt cgaatggttt tgaccggaaa ttcttataca gggcaggcac
     3061 atcagctgca ggtgccggtt cttacactgg acataggcat tgaagatggc gaagctgaca
     3121 atctcaacct gccatccgcc ccgtctgatt tggcgtacat catgtacaca tccggttcaa
     3181 cgggcaaacc gaaaggcgtc atgatcgaac ataaaagcat tctgcgcctc gtcaaaaatg
     3241 ccgggtacgt tcctgttact gaagaggacc gcatggcgca aacaggggca gtcagctttg
     3301 atgccggaac gtttgaggta ttcggcgcac tgctgaatgg cgcagcgctt tatccggtca
     3361 aaaaagagac actgcttgat gcgaaacaat ttgctgcatt cctgcgtgag caaagcatca
     3421 caactatgtg gctgacatca cctttattca accagcttgc tgcaaaggat gcgggtatgt
     3481 tcggcacact gcgccattta atcatcggcg gagatgccct tgtcccgcat attgtcagca
     3541 aagtaaaaca ggcatcgccg tcgctttcat tgtggaacgg atacggcccg acagaaaaca
     3601 cgacgttttc aaccagtttt ctgatcgacc gcgagtatgg cggctctatc ccaatcggga
     3661 agccgatcgg aaattccact gcctatatta tggatgagca gcaatgcctg cagccaatcg
     3721 gcgcgcctgg tgagctttgc gtaggcggaa tcggtgtagc gcgtgggtat gtcaatctcc
     3781 ctgagctgac agaaaagcaa tttctggaag atccgttcag accgggtgag agaatttatc
     3841 gcactggtga cttggcaaga tggctgccgg acggcaatat cgaattttta ggcagaatcg
     3901 acaaccaagt gaaggtgcgc ggcttccgaa ttgagcttgg cgaaattgaa acaaaactga
     3961 acatggctga gcatgtgaca gaagccgcgg tgatcatccg caagaacaaa gcggatgaaa
     4021 atgaaatttg cgcgtacttc acggcggatc gtgaagtggc tgtgggcgag ctgagaaaaa
     4081 cactgtctca gtctttgcct gactatatgg tccctgccca cctgattcaa atggacagcc
     4141 tgccgctcac gccaaacggg aaaatcaaca aaaaggaact gcctgcaccg caatcagaag
     4201 ccgtgcagcc ggaatacgca gcaccagaaa cagagagtga aaagaaatta gcggagatct
     4261 gggaaggaat actcggcgtc agagcaggcg ttactgataa cttctttagg atcggcggcc
     4321 attctttgaa agcgatgatg atgacggcaa aaatccaaga gcattttcat aaggaagttc
     4381 cgataaaagt gctttttgaa aagccgacta ttcaagaact ggcactgtat ttggaagaga
     4441 acgaaagcaa ggaggagcag acgtttgaac cgatcaggca agcatcttat cagcagcatt
     4501 atcctgtatc cccggcccag cggagaatgt atatcctcaa tcagcttgga caagcaaaca
     4561 caagctacaa cgtccccgct gtacttctgc tggagggaga agtagataaa gaccggcttg
     4621 aaaacgcgat tcagcaatta atcaaccggc acgaaatcct ccgtacatcg tttgacatga
     4681 tcgacggaga ggttgtgcaa accgttcata aaaacatatc gttccagctg gaggctgcca
     4741 agggacggga agaagacgcg gaagagataa tcaaagcatt tgttcagccg tttgaattaa
     4801 accgcgcgcc gctcgtccgt tcgaagcttg tccagctgga agaaaaacgc cacctgctgc
     4861 tcattgatat gcatcatatt attactgacg gaagttcaac aggcattcta atcggtgatc
     4921 ttgccaaaat atatcaaggc gcagatctgg aactgccaca aattcactat aaagattacg
     4981 cagtttggca caaagaacaa actaattatc aaaaagatga ggaatactgg ctcgatgtct
     5041 ttaaaggcga actgccaata ctggatcttc ccgcggattt cgagcggcca gctgaacgga
     5101 gctttgcggg agagcgcgtg atgtttgggc ttgataagca aatcacggct caaatcaaat
     5161 cgctcatggc agaaacagat acgacaatgt acatgttttt gctggcggcg ttcaatgtac
     5221 tcctttccaa gtacgcgtca caggatgata tcattgtcgg ctcgccgaca gctggcagaa
     5281 cacatcctga tctgcaaggt gtgccgggta tgtttgtcaa cacggtggca ctcagaacgg
     5341 caccagcggg agataaaacc ttcgcgcaat tccttgaaga ggtcaaaaca gccagccttc
     5401 aagcattcga gcaccagagc tatccgcttg aggagctgat tgaaaagctt ccgcttacaa
     5461 gggatacaag cagaagtccg ctgttcagcg tgatgttcaa catgcagaat atggagattc
     5521 cttcattaag attaggagat ttgaagattt cctcgtattc catgcttcat catgttgcga
     5581 aatttgatct ttccttggaa gcggtcgagc gtgaagagga tatcggccta agctttgact
     5641 atgcgactgc cttgtttaag gacgagacga tccgccgctg gagccgccac tttgtcaata
     5701 tcatcaaagc ggccgcggct aatccgaacg ttcggctgtc tgatgtagat ctgctttcat
     5761 ctgcagaaac ggctgctttg ctagaagaaa gacatatgac tcaaattacc gaagcaacct
     5821 ttgcagcact ttttgaaaaa caggcccagc aaacacctga ccattctgcg gtgaaggctg
     5881 gcggaaatct gttgacctat cgcgagcttg atgaacaggc gaaccagctg gcgcatcatc
     5941 ttcgtgccca aggggcagga aatgaagaca tcgtcgcgat tgttatggac cggtcagctg
     6001 aagtcatggt atccattctc ggtgtcatga aggcgggggc agctttcctt ccgattgatc
     6061 ctgatacacc tgaagaacga atccgttatt cattagagga cagcggagca aaatttgcgg
     6121 tcgtgaatga aagaaacatg acggctattg ggcaatatga agggataatt gtcagccttg
     6181 atgacggtaa atggagaaat gaaagcaagg agcgcccatc atccatttcc gggtctcgca
     6241 atcttgcata cgtcatttat acgtccggta cgaccggaaa gccgaagggc gtgcagattg
     6301 agcatcgcaa tctgacaaac tatgtctctt ggtttagtga agaggcgagc ctgacggaaa
     6361 atgataagac tgcattgctt tcatcttacg catttgacct tggctatacg agcatgttcc
     6421 ccgtacttct gggcgggggc gagctccata tcgtccagaa ggaaacctat acggcgccgg
     6481 atgaaatagc gcactatatc aaggagcatg ggatcactta tatcaagctg acaccgtctc
     6541 tgttccatac aatagtgaac accgccagtt ttgcaaaaga tgcgaacttt gaatccttgc
     6601 gcttgatcgt cttgggagga gaaaaaatca tcccgactga tgttatcgcc ttccgtaaga
     6661 tgtatggaca taccgaattt atcaatcact acggcccgac agaagcaacg atcggcgcca
     6721 tcgccgggcg ggttgatctg tctgagccgg atgcatttgc gaaacgcccg acaatcggac
     6781 gcccgattgc gaatgccggt gcgcttgtct taaatgaagc attgaagctt gtgccgcctg
     6841 gagcgagcgg acagctctat atcacgggac aggggctcgc gagagggtat ctcaacaggc
     6901 ctcagctgac agccgagaga tttgtagaaa atccatattc gccgggaagc ctcatgtaca
     6961 aaaccggaga tgtcgtacga agactttctg acggtacgct tgcatttatc ggccgggctg
     7021 atgatcaggt gaaaatccga ggctaccgca ttgagccgaa agaaattgaa acggtcatgc
     7081 tcagcctcag cggaattcaa gaagcggttg tactagcggt ttccgagggc ggtcttcaag
     7141 agctttgcgc gtattatacg tcggatcaag ctattgaaaa agcagagctc cggtaccagc
     7201 tttccctaac actgccgtct catatgattc ctgctttctt tgtgcaggtt gacgcgattc
     7261 cgctgacggc aaacggaaaa accgacagaa acgctttgcc gaagccgaac gcggcacaat
     7321 ccggaggcaa ggccttggcc gcaccggaga cagcgcttga agaaagctta tgccgcattt
     7381 ggcagaaaac gcttggcata gaagccatcg gcattgatga caattttttc gatttaggcg
     7441 gccattcatt aaaagggatg atgctgattg ccaacattca agcggaattg gagaaaagcg
     7501 taccgcttaa agcactgttc gagcagccga ctgttcgcca gctggcggct tatatggagg
     7561 cgtctgccgt ttcaggcgat catcaagtcc tcaaaccggc tgacaagcag gctatgtatc
     7621 cattgtcatc cgcacagaaa cggatgtacg tgctcaatca gcttgaccgc cggacgataa
     7681 gctacaacat gccatctgtt cttctaatgg aaggagagct tgatatttcg cgcctgcgcg
     7741 actcactcaa tcagcttgtg aaccgtcacg aatcattgcg gacgtcattt atggaagcta
     7801 atggtgagcc tgttcagcgc atcattgaga aggcggaggt tgatcttcat gtgtttgaag
     7861 ccaaagaaga cgaagcggac caaaagatta aggaatttat ccggccattc gacttaaacg
     7921 acgcaccgct cattcgcgca gctttgcttc gaatagaagc gaaaaaacat ttgctgcttt
     7981 tagatatgca tcatatcatc gcagacggcg tctcaagagg catctttgta aaagaattgg
     8041 cgctgcttta caaaggagag cagcttccgg agccgacgct tcattataaa gatttcgccg
     8101 tttggcaaaa tgaagctgag caaaaagaac ggatgaagga gcatgaggcg tactggctgt
     8161 cagttctttc aggcgagctg cctgagcttg atctcccgct cgattatgcc cgtccgcctg
     8221 tgcaaagctt taaaggagat acgatccgtt tccgtacggg aagtgagacg gcaaaggcgg
     8281 tagaaaaact gcttgcggaa accggaacga ccttgcacat ggtgctccat gctgttttcc
     8341 acgtcttttt aagcaaaatt tccggacagc gggatatcgt catcggctcc gttactgccg
     8401 gccggacgaa tgctgatgtt caggacatgc cgggaatgtt cgtcaataca cttgccctga
     8461 gaatggaagc gaaagaacag caaacatttg cggagctttt ggagcaagca aagcagacga
     8521 acctgtcagc gcttgagcat caggagtatc cgtttgaaga tctggttaat cagcttgatc
     8581 tccctcggga tatgagccga aacccattgt ttaatgtgat ggtgacgaca gaaaaccctg
     8641 ataaagaaca gcttacattg caaaatctga gcatttcacc ttatgaggct catcagggaa
     8701 cttctaagtt tgatctgaca ctgggcggat ttactgatga aaatggcatt ggcttgcagc
     8761 tcgaatatgc gacagatctg ttcgcgaaag aaacagttga aaaatggagc gaatacgttc
     8821 tgcggctact aaaggctgtt gcggataacc cgaaccagcc gctttccagc ctgtcactgg
     8881 tcaccgagac agaaaagcaa gcacttcttg aggcatggaa gggtaaagcg ctgcctgtgc
     8941 cgacagacaa aacggttcat cagctattcg aagagactgt ccagcgccac aaagaccgcc
     9001 cggctgtcac atacaacggc cagtcttgga cgtacggtga gctgaatgcg aaggcgaatc
     9061 gtctcgcgcg gattctgatg gactgcggca tcagcccgga tgaccgcgtc ggcattctca
     9121 cgaagccatc gcttgaaatg tccgccgcgg tgctcggcgt cttgaaagcc ggagcggcgt
     9181 ttgtgccgat tgatcctgac tacccggatc agcggattga gtatatttta caggacagcg
     9241 gcgcgaagct tctcttgaaa caggaaggca tttcagtgcc ggacagcttt acgggagatg
     9301 tcattcttct cgacggaagc cgcacgattc taagcctgcc gcttgatgaa aacgacgagg
     9361 gaaatccaga aaccgctgta accgcggaga acttggcgta catgatttac acgtctggaa
     9421 cgaccggaca gccgaagggt gtcatggtcg agcaccatgc gcttgtgaac ctgtgcttct
     9481 ggcaccacga cgcgttcagc atgacagcgg aggaccgcag tgcgaagtac gcgggctttg
     9541 ggttcgacgc ttccatttgg gagatgttcc cgacctggac gatcggtgcc gagcttcacg
     9601 tcattgatga agcgatccgc ctcgatatcg tccgcctgaa cgattatttt gaaacgaacg
     9661 gcgtaacgat cacgttcctg ccgacacagc ttgcggaaca gttcatggag cttgagaaca
     9721 catcacttcg cgtcctcttg actggaggag acaagctgaa gcgggcagtg aaaaagccgt
     9781 acacactcgt caacaactac gggccgacag aaaacacggt cgttgccaca agcgcagaaa
     9841 tccatccgga ggaaggctcg ctttccatcg gacgggcgat cgccaatacg agagtataca
     9901 ttctcggcga gggcaatcag gtgcagccgg aaggcgtagc cggagagctt tgcgtggcgg
     9961 ggcgcggact ggcacgcggc tatctgaatc gagaagacga aaccgcgaag cggtttgtcg
    10021 ctgatccgtt tgtgccgggt gagcgcatgt accgcaccgg cgatttggtg aagtggacag
    10081 gcggcggcat cgaatacatc ggccggatcg accagcaggt caaggtccgc ggctaccgga
    10141 tcgagctctc agaaattgaa gtccagctcg cccagctttc tgaggtgcag gatgcggcgg
    10201 tgacagctgt caaggataaa ggcggcaata cagcgatcgc ggcgtatgtc acaccggaat
    10261 cagctgacat agaagcactg aaatcagcac tgaaggaaac cctgccggat tacatgatcc
    10321 cggcgttctg ggtgacgctg aacgagcttc cggttacggc aaacggcaaa gtcgaccgca
    10381 aagccttgcc tgagccggac atcgaagcgg gaagcggaga atacaaagcg ccgacgaccg
    10441 acatggaaga gctgcttgcc ggcatctggc aggacgtgct tggaatgt
//
