LOCUS       NZ_JAANHO010000023      9722 bp    DNA     linear   CON 06-NOV-2024
DEFINITION  Bacillus sp. EKM208B 101228_scaffold23, whole genome shotgun
            sequence.
ACCESSION   NZ_JAANHO010000023
VERSION     NZ_JAANHO010000023.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. EKM208B
  ORGANISM  Bacillus sp. EKM208B
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Khalaf,E.M. and Raizada,M.N.
  TITLE     Genome assembly of Bacillus sp. EKM208B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Khalaf,E.M. and Raizada,M.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) Plant Agriculture, University of Guelph, 50
            Stone Road, East, Guelph, On N1G 2W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: EvoCAT (Evogene Clustering & Assembly Toolbox) v.
            December-2019
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011800725.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 06:22:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,002
            CDSs (total)                      :: 3,928
            Genes (coding)                    :: 3,848
            CDSs (with protein)               :: 3,848
            Genes (RNA)                       :: 74
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 39 of 80
            Pseudo Genes (incomplete)         :: 55 of 80
            Pseudo Genes (internal stop)      :: 8 of 80
            Pseudo Genes (multiple problems)  :: 20 of 80
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:46:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9722
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAANHO010000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9722
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1425:>9722](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1426..>9722
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..1350
                     /locus_tag="G9F75_RS19620"
                     /old_locus_tag="G9F75_19540"
     CDS             <1..1350
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-286). E-value:
                     7.6e-160. Score: 525.2. Matches aSDomain:
                     nrpspksdomains_G9F75_RS19620_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (291-445). E-value: 7.1e-54.
                     Score: 173.8. Matches aSDomain:
                     nrpspksdomains_G9F75_RS19620_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057766256.1"
                     /locus_tag="G9F75_RS19620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9F75_19540"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_260399154.1"
                     /sec_met_domain="Condensation (E-value: 5e-81, bitscore:
                     260.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FERNFTDKHHWNQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMI
                     YREQKPYYRGLEDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFR
                     AEDGDHLLLAIHHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKE
                     YANTKAFLKEADYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNV
                     HEAYHTEMNDILLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYP
                     MILEMKHADDLSYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDISFNY
                     LGQFNEMSDSGLFTRSGMPSGQSLSPDTEKPNALDIVGFIENGKMTMTFAYHSLEFHEK
                     TIQSFSDSFKGHLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKLMEIL"
     source          1..9722
                     /collection_date="2014"
                     /db_xref="taxon:1683602"
                     /geo_loc_name="Canada: Guelph"
                     /host="cantaloupe delicious variety"
                     /isolation_source="Dry seeds"
                     /mol_type="genomic DNA"
                     /organism="Bacillus sp. EKM208B"
                     /strain="EKM208B"
                     /submitter_seqid="101228_scaffold23"
     region          1..9722
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9722
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        4..861
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS19620_Epimerization.1"
                     /evalue="7.60E-160"
                     /label="G9F75_RS19620_Epimerization.1"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="286"
                     /protein_start="0"
                     /score="525.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="FERNFTDKHHWNQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMI
                     YREQKPYYRGLEDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFR
                     AEDGDHLLLAIHHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKE
                     YANTKAFLKEADYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNV
                     HEAYHTEMNDILLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYP
                     MILEM"
     aSModule        4..1338
                     /domains="nrpspksdomains_G9F75_RS19620_Epimerization.1"
                     /domains="nrpspksdomains_G9F75_RS19620_TIGR01720.1"
                     /incomplete
                     /locus_tags="G9F75_RS19620"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       334..399
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS19620_0001"
                     /evalue="1.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="132"
                     /protein_start="110"
                     /score="39.6"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       808..855
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS19620_0002"
                     /evalue="1.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="284"
                     /protein_start="268"
                     /score="26.8"
                     /tool="antismash"
                     /translation="ITRTVGWFTSMYPMIL"
     aSDomain        877..1338
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS19620_TIGR01720.1"
                     /evalue="7.10E-54"
                     /label="G9F75_RS19620_TIGR01720.1"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="445"
                     /protein_start="291"
                     /score="173.8"
                     /tool="antismash"
                     /translation="LSYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDI
                     SFNYLGQFNEMSDSGLFTRSGMPSGQSLSPDTEKPNALDIVGFIENGKMTMTFAYHSLE
                     FHEKTIQSFSDSFKGHLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKL"
     CDS_motif       901..957
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS19620_0003"
                     /evalue="3.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="318"
                     /protein_start="299"
                     /score="29.0"
                     /tool="antismash"
                     /translation="KEDIRHIPNKGVGYGILRY"
     CDS_motif       1003..1032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS19620_0004"
                     /evalue="3.00E-03"
                     /label="NRPS-E7"
                     /locus_tag="G9F75_RS19620"
                     /protein_end="343"
                     /protein_start="333"
                     /score="10.1"
                     /tool="antismash"
                     /translation="PDISFNYLGQ"
     aSDomain        1426..2289
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20210_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="8.30E-105"
                     /label="G9F75_RS20210_Condensation_DCL.1"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="288"
                     /protein_start="0"
                     /score="342.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVMDG"
                     /tool="antismash"
                     /translation="MLYHALLDPHSSSYFTQLELRIHGSFQLELFEKSVNELIRTYDIL
                     RTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYIERYKRSVQQQGFHLAKDI
                     LFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNYEALRAGRPAGGSQGKPYS
                     DYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKDYQNEEYSFVWDEELVAQI
                     QQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGRPSEINGIETMAGLFINTI
                     PVRIKID"
     aSModule        1426..4413
                     /complete
                     /domains="nrpspksdomains_G9F75_RS20210_Condensation_DCL.1"
                     /domains="nrpspksdomains_G9F75_RS20210_AMP-binding.1"
                     /domains="nrpspksdomains_G9F75_RS20210_PCP.1"
                     /locus_tags="G9F75_RS20210"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            1426..>4416
                     /gene="srfAB"
                     /locus_tag="G9F75_RS20210"
                     /pseudo=""
     CDS             1426..>4416
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (0-288). E-value:
                     8.3e-105. Score: 342.1. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20210_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (452-846). E-value:
                     4.7e-113. Score: 369.8. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20210_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (952-996). E-value: 1.1e-14. Score:
                     46.4. Matches aSDomain: nrpspksdomains_G9F75_RS20210_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="G9F75_RS20210"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 4.5e-85, bitscore:
                     273.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.7e-121, bitscore:
                     391.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.3e-09, bitscore:
                     25.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLYHALLDPHSSSYFTQLELRIHGSFQLELFEKSVNELIRTYDIL
                     RTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYIERYKRSVQQQGFHLAKDI
                     LFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNYEALRAGRPAGGSQGKPYS
                     DYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKDYQNEEYSFVWDEELVAQI
                     QQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGRPSEINGIETMAGLFINTI
                     PVRIKIDKDAAFSDVMAAVQKNAVEAERYDYVPLYDIQKRSALDGSLLNHLVAFENYPL
                     DKELENGGMEERLGFSIKVEHAFEQTSFDFNLIVYPGKTWTVKIKYNGAAFAYDAIERT
                     AHHLTRMMKAAVGTPDAPVRELGLVSGEEERQIVEVFNDTKTALPEEEAVHRLFEAQAN
                     RTPASIAIKEAGREWTYREVNEAANRLARHLVKSGLEKGRTAAIMNDRSAETVVGMLAV
                     LKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQEQAGALQVPVVMLDESADETV
                     SGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIRLVKHSNYVPVHEEDRMAQTG
                     AVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFLKEQRITTMWLTSPLFNQLAQ
                     KDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNGYGPTENTTFSTSFLIDQDYD
                     GSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGVARGYVNLPELTEKQFVRDPF
                     RPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVRGFRIELGEIEAKISQAENVTESAAV
                     IRKNKADENEICAYFTADQALSPEDLRKTLSESLPEYMIPAHFIQMNQFPLTANGKIDK
                     KELPEPQAEAVQKEYVAPKTEAEQKLADIWEGILGVKAGVTDNFFTIGGHSLKAMMMTA
                     KIQEHFQK"
     CDS_motif       1462..1578
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0001"
                     /evalue="2.30E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="51"
                     /protein_start="12"
                     /score="45.6"
                     /tool="antismash"
                     /translation="SYFTQLELRIHGSFQLELFEKSVNELIRTYDILRTVFVH"
     CDS_motif       1795..1860
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0002"
                     /evalue="3.50E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="145"
                     /protein_start="123"
                     /score="38.7"
                     /tool="antismash"
                     /translation="NHHIVMDGWSMGVLMKSLFQNY"
     CDS_motif       2182..2277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0003"
                     /evalue="7.20E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="284"
                     /protein_start="252"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       2326..2442
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0004"
                     /evalue="2.20E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="339"
                     /protein_start="300"
                     /score="55.5"
                     /tool="antismash"
                     /translation="QKNAVEAERYDYVPLYDIQKRSALDGSLLNHLVAFENYP"
     aSDomain        2782..3963
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20210_AMP-binding.1"
                     /evalue="4.70E-113"
                     /label="G9F75_RS20210_AMP-binding.1"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="846"
                     /protein_start="452"
                     /score="369.8"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEAQANRTPASIAIKEAGREWTYREVNEAANRLARHLVKSGLEKG
                     RTAAIMNDRSAETVVGMLAVLKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQE
                     QAGALQVPVVMLDESADETVSGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIR
                     LVKHSNYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFL
                     KEQRITTMWLTSPLFNQLAQKDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNG
                     YGPTENTTFSTSFLIDQDYDGSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGV
                     ARGYVNLPELTEKQFVRDPFRPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVR"
     CDS_motif       2914..2967
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0005"
                     /evalue="1.90E+01"
                     /label="C5_DCL_263-294"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="514"
                     /protein_start="496"
                     /score="-2.1"
                     /tool="antismash"
                     /translation="GRTAAIMNDRSAETVVGM"
     CDS_motif       2968..3009
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0006"
                     /evalue="2.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="528"
                     /protein_start="514"
                     /score="20.5"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPID"
     CDS_motif       3196..3255
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0007"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="610"
                     /protein_start="590"
                     /score="35.7"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       3757..3846
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0008"
                     /evalue="2.20E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="807"
                     /protein_start="777"
                     /score="48.7"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGSGVARGYVNLPELTEKQF"
     CDS_motif       3928..3993
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20210_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="856"
                     /protein_start="834"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4282..4413
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20210_PCP.1"
                     /evalue="1.10E-14"
                     /label="G9F75_RS20210_PCP.1"
                     /locus_tag="G9F75_RS20210"
                     /protein_end="996"
                     /protein_start="952"
                     /score="46.4"
                     /tool="antismash"
                     /translation="EQKLADIWEGILGVKAGVTDNFFTIGGHSLKAMMMTAKIQEHFQ"
     misc_feature    complement(4463..4479)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.97"
                     /tool="antismash"
     gene            4481..4645
                     /gene="comS"
                     /locus_tag="G9F75_RS20215"
     CDS             4481..4645
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: protein motif:HMM:NF036673.5"
                     /locus_tag="G9F75_RS20215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_012116773.1"
                     /transl_table=11
                     /translation="MRARRSSSLNRSDKRLIRSIILFPQHSAGCISLISSDRPARATTS
                     LLYFCWKGQ"
     aSDomain        4567..5382
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20245_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.00E-97"
                     /label="G9F75_RS20245_Condensation_LCL.1"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="272"
                     /protein_start="0"
                     /score="318.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKKRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNRAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSIWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSH"
     aSModule        4567..7608
                     /complete
                     /domains="nrpspksdomains_G9F75_RS20245_Condensation_LCL.1"
                     /domains="nrpspksdomains_G9F75_RS20245_AMP-binding.1"
                     /domains="nrpspksdomains_G9F75_RS20245_PCP.1"
                     /locus_tags="G9F75_RS20245"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     gene            4567..>9722
                     /gene="srfAB"
                     /locus_tag="G9F75_RS20245"
                     /pseudo=""
     CDS             4567..>9722
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-272). E-value:
                     1e-97. Score: 318.7. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20245_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (437-839). E-value:
                     4.7e-116. Score: 379.7. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20245_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (945-1014). E-value: 4.5e-23. Score:
                     73.2. Matches aSDomain: nrpspksdomains_G9F75_RS20245_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1029-1317). E-value:
                     2.3e-104. Score: 340.5. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20245_Condensation_LCL.2"
                     /NRPS_PKS="Domain: AMP-binding (1482-1717). E-value:
                     8.5e-62. Score: 200.9. Matches aSDomain:
                     nrpspksdomains_G9F75_RS20245_AMP-binding.2"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="G9F75_RS20245"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 3.3e-125, bitscore:
                     405.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.6e-17, bitscore:
                     50.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.8e-96, bitscore:
                     308.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKKRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNRAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSIWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSHPAGKKTFKQ
                     FLDEVKTASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNMQNMEIPALRLGDLE
                     ISSYSMHHHVAKFDLSLEAAERGEEVGLSFDYAKALFADETIRRWSAHFVNLIKACAEN
                     PDIQLSDASLLSAPEREALLSDEKRTEADLPEGTFVSLFERQAQKTPDLTAVAGGTSLT
                     YRELDERSNRFARHLQACGTGSEDIVAIMMDRSADLITAILGVMKAGAAFLPIDPETPE
                     ERIRYSLEDSGTKLLVVNERNMTAAAVYKGKTIVMEDGEWQNESAGRLETKPGADQLAY
                     IIYTSGTTGKPKGVQLEHRNLINYVTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPIL
                     QAGGELHIVPKETYTAPDQLGEYIQKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRL
                     VVLGGEKIITSDVRKFHDVYAHTDFINHYGPTETTIGAIAGRINMERLDQYEQRPVIGR
                     PIANTGALVLDGAMQLVPPGASGELYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMY
                     KTGDVVRRLSDGTIEFIGRADDQVKIRGYRIELKEVETVLLSVNGIQEAVVLAVSEGGL
                     PELCAYYKAGSELKGSELRKRLSETLPSHMLPAYFVQVDRIPLTANGKTDKNALPKPGV
                     SQTAQTASALPETELEEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAEL
                     DKTVPLKALFEQPTVRLLAAYIEKSAVSEGYRMITPADSADTYPLSSAQKRMYVLNQLD
                     RETISYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSFIDQNGRPMQQIAEQADID
                     LHIFEAADEEKADLIIQAFIKPFDLSAAPLIRAALVRLNEKKHLLLMDMHHIIADGVSR
                     SMLVKELAHLYKGGSLPSPNLHYKDFAVWQNEPEQAERMKDHERYWLSAFSGELPELNL
                     PTDFPRPPVQSFKGQYVRFRAGRETEKAVRELMESSGATLHMVLHAAFHVFLSKITGQR
                     DIIIGSVTAGRTNAEVQEMPGMFVNTLALRNETRKEQTFAGLLERVKQTNLDALAHQDY
                     PFEDLIGKLDLSRDMSRNPLFQVMVTTEDPDKETLELENLRITPYESNQGTAKFDLTLG
                     GFTDQEGLGLQFEYATDLFKKETIEKWSAGFLRILKQAAESPDRKLPELSLISDAEKQA
                     LLNAWKGKTLSVPQDKTVHRLFEETAARYADRPAAAYNGAKWTYGELNARANRIARILM
                     DCGVTADERVGILTKPSLEMAAGVLGVLKAGAAFVPIDPDYPEERISYILQDSGAKLLL
                     TQEALGVPDSYNGETILLDGGRSILSLPLDENDEANPQTETTADHLAYMIYTSGTTGQP
                     KGVMVEHHALVNLCFWHHDAFAMTADDKSAKYAGFGFDASIWEMFPAWTIGAELHVIDE
                     AIRLDITRLNHYFEEHGVTIT"
     CDS_motif       4600..4713
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0001"
                     /evalue="8.40E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="49"
                     /protein_start="11"
                     /score="50.3"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGSVDKKRLEEAMQALINRHETLRTSF"
     CDS_motif       4909..4974
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0002"
                     /evalue="1.60E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="136"
                     /protein_start="114"
                     /score="30.2"
                     /tool="antismash"
                     /translation="MHHIITDGSSMGIFIGDLAKLY"
     CDS_motif       5005..5040
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0003"
                     /evalue="2.90E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="158"
                     /protein_start="146"
                     /score="13.5"
                     /tool="antismash"
                     /translation="HYKDFSIWQREK"
     CDS_motif       5287..5376
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0004"
                     /evalue="1.10E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="270"
                     /protein_start="240"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIIVGSPAAGRQHPDLQDVPGMFVNTVALR"
     CDS_motif       5431..5544
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0005"
                     /evalue="3.80E-15"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="326"
                     /protein_start="288"
                     /score="48.2"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNM"
     aSDomain        5878..7083
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20245_AMP-binding.1"
                     /evalue="4.70E-116"
                     /label="G9F75_RS20245_AMP-binding.1"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="839"
                     /protein_start="437"
                     /score="379.7"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FERQAQKTPDLTAVAGGTSLTYRELDERSNRFARHLQACGTGSED
                     IVAIMMDRSADLITAILGVMKAGAAFLPIDPETPEERIRYSLEDSGTKLLVVNERNMTA
                     AAVYKGKTIVMEDGEWQNESAGRLETKPGADQLAYIIYTSGTTGKPKGVQLEHRNLINY
                     VTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPILQAGGELHIVPKETYTAPDQLGEYI
                     QKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRLVVLGGEKIITSDVRKFHDVYAHTD
                     FINHYGPTETTIGAIAGRINMERLDQYEQRPVIGRPIANTGALVLDGAMQLVPPGASGE
                     LYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMYKTGDVVRRLSDGTIEFIGRADDQV
                     KIR"
     CDS_motif       6061..6102
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0006"
                     /evalue="6.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="512"
                     /protein_start="498"
                     /score="16.2"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       6289..6348
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0007"
                     /evalue="5.30E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="594"
                     /protein_start="574"
                     /score="31.2"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGKPKGVQLEH"
     CDS_motif       6877..6966
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0008"
                     /evalue="7.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="800"
                     /protein_start="770"
                     /score="50.2"
                     /tool="antismash"
                     /translation="PPGASGELYITGKGLARGYLHRPQLTAEKF"
     CDS_motif       7048..7113
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0009"
                     /evalue="1.50E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="849"
                     /protein_start="827"
                     /score="36.2"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIELKEVE"
     aSDomain        7402..7608
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20245_PCP.1"
                     /evalue="4.50E-23"
                     /label="G9F75_RS20245_PCP.1"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1014"
                     /protein_start="945"
                     /score="73.2"
                     /tool="antismash"
                     /translation="EEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAELD
                     KTVPLKALFEQPTVRLLAAYIEKS"
     aSDomain        7654..8517
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20245_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.30E-104"
                     /label="G9F75_RS20245_Condensation_LCL.2"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1317"
                     /protein_start="1029"
                     /score="340.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DTYPLSSAQKRMYVLNQLDRETISYNMPSVLLMEGEVNISRLQEA
                     LNQMINRHESLRTSFIDQNGRPMQQIAEQADIDLHIFEAADEEKADLIIQAFIKPFDLS
                     AAPLIRAALVRLNEKKHLLLMDMHHIIADGVSRSMLVKELAHLYKGGSLPSPNLHYKDF
                     AVWQNEPEQAERMKDHERYWLSAFSGELPELNLPTDFPRPPVQSFKGQYVRFRAGRETE
                     KAVRELMESSGATLHMVLHAAFHVFLSKITGQRDIIIGSVTAGRTNAEVQEMPGMFVNT
                     LALRNET"
     aSModule        7654..9717
                     /domains="nrpspksdomains_G9F75_RS20245_Condensation_LCL.2"
                     /domains="nrpspksdomains_G9F75_RS20245_AMP-binding.2"
                     /incomplete
                     /locus_tags="G9F75_RS20245"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       7723..7833
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0010"
                     /evalue="8.90E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1089"
                     /protein_start="1052"
                     /score="50.3"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSF"
     CDS_motif       8032..8097
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0011"
                     /evalue="7.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1177"
                     /protein_start="1155"
                     /score="28.1"
                     /tool="antismash"
                     /translation="MHHIIADGVSRSMLVKELAHLY"
     CDS_motif       8128..8160
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0012"
                     /evalue="2.20E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1198"
                     /protein_start="1187"
                     /score="13.9"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8419..8508
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0013"
                     /evalue="7.30E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1314"
                     /protein_start="1284"
                     /score="44.1"
                     /tool="antismash"
                     /translation="DIIIGSVTAGRTNAEVQEMPGMFVNTLALR"
     CDS_motif       8557..8670
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0014"
                     /evalue="1.10E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1368"
                     /protein_start="1330"
                     /score="59.6"
                     /tool="antismash"
                     /translation="KQTNLDALAHQDYPFEDLIGKLDLSRDMSRNPLFQVMV"
     aSDomain        9013..9717
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9F75_RS20245_AMP-binding.2"
                     /evalue="8.50E-62"
                     /label="G9F75_RS20245_AMP-binding.2"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1717"
                     /protein_start="1482"
                     /score="200.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEETAARYADRPAAAYNGAKWTYGELNARANRIARILMDCGVTAD
                     ERVGILTKPSLEMAAGVLGVLKAGAAFVPIDPDYPEERISYILQDSGAKLLLTQEALGV
                     PDSYNGETILLDGGRSILSLPLDENDEANPQTETTADHLAYMIYTSGTTGQPKGVMVEH
                     HALVNLCFWHHDAFAMTADDKSAKYAGFGFDASIWEMFPAWTIGAELHVIDEAIRLDIT
                     RLNHYFEEHGVTI"
     CDS_motif       9199..9240
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0015"
                     /evalue="1.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1558"
                     /protein_start="1544"
                     /score="17.9"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       9442..9501
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G9F75_RS20245_0016"
                     /evalue="1.40E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="G9F75_RS20245"
                     /protein_end="1645"
                     /protein_start="1625"
                     /score="33.0"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
ORIGIN
        1 tttgaacgaa actttaccga caaacaccac tggaatcaat ccgtcatgct tcacgcgaaa
       61 gacggctttg atccggagat aacagaaaaa acattacatg tcctgacggt gcaccatgat
      121 gcgctccgga tgatttatcg tgaacaaaaa ccgtattaca gaggacttga ggatgtgtct
      181 gttgaactga acgtctttga gctgaacgga cctgctgaag accatgagga ccgtatcgaa
      241 agggaagcag accgtcttca aagcagtatt tcactggaaa caggacatct gctgaaagcc
      301 gggctcttcc gggctgaaga tggagaccac cttcttctcg caatccatca cttagtcgtg
      361 gatggtgtat cttggcggat tttactggag gacttcacgt ccgtttatac gcagctgaag
      421 caaggcaatg aaccggcgct gccgccgaaa acacattcat tcgccgaatt tgctgagaga
      481 atcaaagaat acgcaaatac gaaggcgttt ctgaaagaag cggattactg gagagagctt
      541 gaggaaaaag aggtatgcac tcagcttccg aaagacaggc agtctggtga tcagcgcatg
      601 aaacatacga gaacggtcag cttctctctg acgcctgaac aaactgaaca gctgacgaca
      661 aacgtacatg aagcctatca caccgaaatg aacgacatcc tgctcacggc gctcggactg
      721 gcgctgaaag agtggacggg tgaagacacg atcggcgttc atttggaagg ccacgggcgc
      781 gaagacattc ttgacggctt gaatatcacc cggacggtcg gatggtttac gagcatgtat
      841 ccgatgatcc ttgagatgaa gcacgccgac gatctttcat atcagctgaa acaaatgaaa
      901 gaagacatca gacacattcc gaacaaagga gtcggctacg gcattctgcg ttatgtaacg
      961 gcgcccgagc ataaagagaa tctttcattt gagattgatc cggatatcag ctttaactac
     1021 ttaggccagt ttaatgagat gtcggattcc ggcttattta caagatccgg gatgccgtca
     1081 ggacaatcgc tgagccctga cacagagaag ccgaatgcgc ttgatattgt cggatttata
     1141 gaaaatggga agatgacgat gacgtttgcc tatcattctc tcgaatttca tgaaaaaacc
     1201 attcaatcgt tcagtgacag ctttaaaggg cacctcttga aaatcataga tcactgcctg
     1261 gcccaagacg gacctgagct tacgcctagc gatcttggcg atgatgatct gacgcttgat
     1321 gaacttgata aattaatgga aattctctaa cagaaaagac agaggtgaca tatgagcaaa
     1381 aaatcaatcc aaaaggtgta tgcactcaca ccgatgcagg agggaatgct gtatcatgcg
     1441 ctgcttgatc cgcattcttc ctcctacttc acacaattag agcttaggat tcacggaagc
     1501 tttcagctgg agctttttga aaaaagcgtc aatgagctga tccggacata tgacatcctg
     1561 cgtacggtat tcgtgcacca gcagcttcaa aaaccgcgcc aagtcgtatt ggcggagcgg
     1621 aagacgaagg tgcactatga agatattagt caattagatg aagcgcgcca aaccgaatat
     1681 attgaacgct ataaacgcag cgtgcagcag cagggctttc atctggcgaa ggatattctc
     1741 tttaaagcgg cggtgttcag actcagtgag aaggagctgt atctcgtctg gagcaatcat
     1801 catatcgtca tggacggctg gagtatgggc gttttgatga aaagcctgtt ccaaaactat
     1861 gaagcgcttc gtgccggccg gcccgccggg gggagccagg gcaaacctta ttctgactat
     1921 atcaaatggc tcggaggccg ggattatgag gaggcggaac aatattggag cagccgcctg
     1981 gcggatttcg aacagccgag cctgctcccg ggccgtctgg cagcagaaaa gaaagactac
     2041 caaaatgaag aatattcttt tgtctgggat gaggagctgg tcgcgcaaat tcagcagacc
     2101 gccaaccggc atcaagtcac ggggcctaac ctgtttcagg ccgtttgggg tgcggtgctc
     2161 agcaaatata actatacaga cgatgtggtg ttcggcaccg tcgtatcagg ccgtccttca
     2221 gaaatcaacg gcattgaaac gatggcgggg ctgttcatca atacgattcc cgtcagaatc
     2281 aagattgaca aagatgcagc cttttcggac gtgatggcgg cagttcagaa aaacgccgtg
     2341 gaagccgagc gctatgatta tgtgccgctc tatgacattc aaaagcgctc cgccctagac
     2401 ggcagcctct tgaatcattt ggtcgcgttt gaaaactatc cgctggacaa agagctggaa
     2461 aacgggggca tggaggaaag actcggcttt tccattaaag tggaacatgc ttttgagcag
     2521 acgagctttg atttcaatct catcgtgtat ccgggaaaaa cgtggactgt caaaatcaaa
     2581 tacaacggag cggctttcgc ttatgatgcc atcgaacgaa cggcgcacca tctgacccgc
     2641 atgatgaaag ccgcggtcgg aacgcctgac gcgcccgtgc gggaactcgg ccttgtttca
     2701 ggtgaagaag agcggcagat tgttgaggta ttcaatgata cgaaaacggc gcttccggaa
     2761 gaggaggccg ttcaccgttt gtttgaagca caggcaaacc ggacgcctgc aagtatcgcg
     2821 ataaaagaag cgggacgcga atggacctac cgtgaagtga acgaagcggc caaccgcctg
     2881 gcgagacacc ttgtgaagag cgggctggaa aaaggccgga ccgccgctat tatgaacgac
     2941 cggtctgcgg aaaccgttgt aggaatgctt gccgtcctaa aagcaggcgg cgcatatgtg
     3001 ccgattgatc cggcttttcc ggaggaccgt ctccgcttta tggcggaaga cagctcgatt
     3061 cggctcgtgc tgacagttca ggcatatcaa gaacaagcgg gcgcattgca agtcccggtc
     3121 gtcatgctgg atgaaagcgc ggatgaaacg gtaagcggaa cagacttgaa tctccccgct
     3181 ggcggcaacg acttggcgta tatcatgtat acatccggat cgaccggcaa accgaaaggc
     3241 gtcatgattg aacacagaaa tatcatcagg ctcgtcaaac attcgaatta cgtgccggtt
     3301 catgaagaag accggatggc gcaaacggga gccgtcagct ttgatgccgg aaccttcgaa
     3361 gtcttcggcg cattgctgaa cggagcgtca ttgcacccgg tgaaaaaaga gacgctgctt
     3421 gacgccggac aattcgccca atttctgaaa gagcagcgga tcacgaccat gtggctgacg
     3481 tctccgctgt ttaatcagct tgcccaaaag gatgccggca tgtttaacac gctccggcac
     3541 ctcatcatcg gcggtgatgc acttgtgccg catatcgtca gcaaagtgag aaaggcatca
     3601 ccggagctgt cgctttggaa cggctacggg ccgacagaga atacgacgtt ttcgacgagt
     3661 tttctcattg atcaggacta cgacggctcg atcccgatcg ggaagccgat cggaaattcc
     3721 actgcgtaca ttatggatga aaaccgcaac ctccagccga tcggcgcgcc cggtgagctg
     3781 tgcgtcggcg gaagcggagt ggcaagaggc tatgtgaatc tgcctgaatt aacggagaag
     3841 cagtttgtac gcgatccgtt cagaccggat gaaatgatat accggacggg ggacttggcg
     3901 aaatggcttc cggacggcac gatcgagttt ctcggcagaa ttgacaacca agtaaaggtc
     3961 cgcggtttca gaatcgagct cggtgaaatc gaggcgaaaa tcagccaggc ggagaatgtg
     4021 acggaatctg cggctgtgat ccggaaaaat aaagcggatg aaaatgaaat ctgcgcttac
     4081 tttaccgcag accaagccct ttcgccggaa gacctgcgca aaacgctttc ggaatcactt
     4141 ccggaataca tgattccggc gcacttcatc cagatgaatc agtttccgct gacggcgaac
     4201 ggaaagattg ataaaaaaga gctgcctgag cctcaggctg aagccgttca aaaagaatac
     4261 gtagcgccga aaacggaagc ggagcagaaa ctcgcggaca tttgggaagg cattctcggt
     4321 gtaaaagcgg gtgtgactga caatttcttc acgattggcg gacattcttt aaaagccatg
     4381 atgatgaccg ctaaaatcca ggagcacttt caaaaagaag tgccgattaa agtgttattt
     4441 gaaaagccga ccattcagga gcttgcgcat tatttgcagc atgagagcga ggaggagcag
     4501 cagtttgaac cgatccgaca agcgccttat caggagcatt atcctgtttc ctcagcacag
     4561 cgcaggatgt atatccttaa tcagctcgga caggccagca cgagctacaa cgtccctgct
     4621 gtacttttgc tggaagggtc agtagacaaa aagcgtcttg aggaggccat gcaggcatta
     4681 atcaaccgtc atgagacact gcgtacgtcg tttgacatgg cagatggaga agtcgtgcag
     4741 accattcata aaaatgtgtc gtttgagctt gaaaccgccg agggccggga agaagatgca
     4801 gaagagctga caaaagcctt tatcaggccg tttgcgctca atcgtgcgcc gctggtccgt
     4861 tcgaagctga tccggcttga agaagaccgg catcttctgc tgattgacat gcaccacatt
     4921 attacggacg ggagctcaat gggcattttc atcggtgatc ttgcgaagct ttatcaaggc
     4981 acggagcttg agctgccaaa gattcattat aaggattttt caatctggca gcgtgaaaaa
     5041 gcaaatctcg atcagcacga agcctactgg cttgatacgt tcaaaggcga tctgccggtg
     5101 ctggatctgc cgcttgattt cccgcgtcct gccgagcgca gttttgaagg ggaacgcgtc
     5161 atcttcgggc ttgataaaca ggtgacggcg cagattaaga agctgctggc tgatacggat
     5221 acgacgatgt acatgttctt gttggccgct tttcaagtgc tgctgtccaa atattcgggg
     5281 caggaagaca tcatagtcgg gtctccggcg gccggaagac agcatcctga tctccaagac
     5341 gtgccgggca tgtttgtcaa cacagttgcg cttcggtcgc atcctgccgg caaaaaaacg
     5401 ttcaaacaat ttttggatga ggtgaaaacg gcgagccttc aagcttttga gcatcaaagc
     5461 tatccgcttg aagaattaat tgaaaaactg ccgttaacga gggacacaag ccggagtccg
     5521 ctgttcagcg tgttatttaa tatgcagaac atggagatcc cggccctgcg gctcggagat
     5581 ttggagattt cttcatactc catgcatcat catgtcgcta aatttgatct ttccttagaa
     5641 gcggctgaac gcggagaaga ggtcggattg agctttgatt acgcgaaagc cttatttgcg
     5701 gacgaaacca tccgccgctg gagcgctcat tttgtcaatc tgattaaagc ctgcgctgaa
     5761 aatccggata ttcagctgtc cgacgcaagc ctgctgtccg ctcctgaacg ggaagcgctc
     5821 ctttctgatg aaaaacgtac ggaagcggat ttgcctgagg gcacttttgt ttcgctgttt
     5881 gaacggcaag cgcaaaaaac gcctgatctc acggcggttg cgggcggaac aagtctgaca
     5941 tatcgtgagc ttgatgaacg ctcgaaccgg tttgcccgcc accttcaggc ttgcggaacg
     6001 ggcagtgagg acatcgtggc cattatgatg gatcgttcgg ccgacttgat taccgcgatt
     6061 ctcggcgtca tgaaagccgg agcggcattt ctcccgattg atccggagac tcctgaagag
     6121 agaatccgct actctcttga agacagcgga acgaagcttc tggtcgtcaa tgagagaaac
     6181 atgaccgcgg ccgccgttta taaaggaaaa acgatcgtaa tggaagacgg agaatggcag
     6241 aacgaaagcg ccggccggct tgaaacgaag cccggcgccg accagcttgc ctatatcatt
     6301 tatacctcgg gaacaacggg caaaccgaaa ggcgtccagc tggagcaccg caatctaatc
     6361 aattatgtca catggttcag ccgtgaagcc ggtttgactg aagctgacaa atctgtgctg
     6421 ctgtcttctt acgcatttga tctcggctat acggctatat tcccgattct tcaggcgggc
     6481 ggagagctgc acattgtgcc gaaagagacg tacaccgcgc ctgatcagct tggtgagtat
     6541 atacagaaaa acggcattac gtatatgaaa ttgacgccgt cattgttcca tatgatcgtc
     6601 aatacagccc gttttacgtc agaacgccgt ttcagcccgc ttcgtttagt ggtgctcggc
     6661 ggagaaaaga tcatcacgtc cgatgtccgc aagtttcatg acgtatacgc ccataccgac
     6721 ttcatcaatc actacgggcc gacggaaacg acgatcggcg ccattgccgg acggatcaat
     6781 atggagcgcc ttgatcaata tgagcagcgt cccgtcatcg gccgcccgat cgcgaatacc
     6841 ggcgcgcttg tattggacgg agcaatgcag ctcgtgcctc cgggcgcaag cggcgagctt
     6901 tatattaccg gaaaggggct tgccagagga tatcttcacc gtccgcagct gacggcggag
     6961 aaatttctct caaatccttt ttcaccggac agcctgatgt acaaaacggg agatgtcgtc
     7021 cgcaggcttt ctgacgggac gattgaattt atcggccgtg cggatgatca ggtgaaaatc
     7081 cggggctacc gcattgaatt aaaagaggtt gaaaccgtgc tgttaagcgt aaacggcatt
     7141 caggaggcgg tcgttctcgc agtcagcgaa ggcgggctgc cggaactgtg cgcgtactat
     7201 aaagccggca gcgagctgaa aggctctgag cttcgcaaac ggctttccga aacactgccg
     7261 tctcatatgc tcccggccta tttcgtgcaa gtagaccgca ttccgctcac agccaacgga
     7321 aaaaccgata aaaacgccct cccgaaaccg ggcgtcagcc aaacggcgca aacagcttca
     7381 gccttgccgg aaacggaatt ggaagaaaag ctgtgccgca tttggaaaca gacactcggt
     7441 acggatacgc tcggcatcga cgataatttc ttcgattacg gcgggcactc tttaaaaggc
     7501 atgatgctcc tcgccaatat tcaggctgaa ctggacaaaa cggttccttt aaaagcgctg
     7561 ttcgaacagc cgactgtccg cctgcttgcc gcatatatcg aaaaatcagc ggtatctgag
     7621 ggatatcgga tgatcacgcc ggccgacagt gcagacacgt acccgttatc atccgctcaa
     7681 aaacggatgt acgtcctgaa ccagcttgac cgggagacaa tcagctataa tatgccgtcc
     7741 gttcttctga tggaaggaga agtcaatatt tcaaggcttc aggaagcgct caatcagatg
     7801 atcaatcgcc acgagtcttt gcggacgtcc tttatcgatc aaaacggccg cccgatgcag
     7861 cagatcgcag aacaggctga catcgacctg cacatctttg aagcagcgga cgaggagaaa
     7921 gcggatctta tcattcaggc attcattaag ccgtttgatc tgagcgcggc tccgctcatt
     7981 cgggccgctc ttgtcagact gaacgaaaag aaacacctgc tgctgatgga tatgcatcat
     8041 attattgccg acggcgtatc cagaagcatg ctggtcaaag agctcgctca tctttataaa
     8101 gggggaagtc tgccgtcgcc gaacctgcac tataaagact tcgccgtctg gcagaatgaa
     8161 cctgagcagg ccgaacggat gaaagaccat gagcgttatt ggctctccgc attttccggc
     8221 gagcttcctg aattgaatct gccgaccgat tttccccgtc cgccggttca aagttttaaa
     8281 ggacaatacg tccgtttcag agcggggcgt gaaacggaaa aagcggtgcg tgaattaatg
     8341 gaatcatcag gagcgactct tcatatggtg cttcacgccg cgttccatgt ctttttgagc
     8401 aaaatcacgg ggcagcgcga tatcattatc ggctcggtga ccgccggaag aacgaacgcc
     8461 gaagttcagg aaatgccggg gatgtttgtc aatacgcttg cgcttcgcaa tgaaacgcgc
     8521 aaagagcaga ccttcgccgg gctgctcgaa cgggtgaaac aaaccaatct cgacgcgctg
     8581 gcgcatcagg attatccgtt tgaggatctg atcggaaagc ttgacctgtc gagagatatg
     8641 agccgaaatc cgttgttcca agtcatggtg acgacagaag atccggataa agaaacactg
     8701 gaactggaga acctgcgcat cactccgtat gagtcaaacc aaggcacggc gaaattcgat
     8761 ctgacactcg gcggatttac ggatcaagaa ggcctcggcc ttcagtttga atatgcgact
     8821 gatctgttta aaaaagaaac aattgaaaag tggagcgccg ggttcctgcg gattctgaag
     8881 caagcggcag aaagcccgga cagaaagctg cctgagcttt cactgatcag cgatgctgaa
     8941 aaacaagctc tcctcaacgc atggaaagga aaaacactct ctgtgccgca ggacaaaacg
     9001 gttcaccgtc tctttgaaga aacggccgcc cgctacgcgg accggccggc cgcggcatac
     9061 aacggcgcga aatggacgta cggcgagctg aacgcaaggg cgaatcgcat cgcgcgcatt
     9121 ctcatggact gcggcgtcac ggctgacgag cgtgtcggca tcctgacgaa accgtccttg
     9181 gaaatggccg cgggcgtact cggcgtcctg aaagcgggcg cggcatttgt gccgattgac
     9241 cccgactatc cggaagagcg gatcagctac attctgcagg acagcggcgc caagcttctt
     9301 ctcacacagg aagcgctggg cgtgccggac agctacaacg gagaaacgat cctgcttgac
     9361 ggcggacgct ccattctgag cctgccgctt gatgaaaacg atgaagcgaa cccgcagacg
     9421 gaaacaacgg cggatcatct cgcttatatg atttacacat caggaacgac cggacagccg
     9481 aaaggtgtca tggtcgaaca ccatgcgctg gtgaacctgt gcttctggca tcacgacgca
     9541 ttcgccatga cggcggatga taaaagcgcc aaatacgcgg gcttcggttt tgacgcctcc
     9601 atctgggaga tgttcccggc atggaccatc ggcgcggagc ttcatgtcat tgacgaagcg
     9661 atccggctgg atatcacccg cttaaatcac tatttcgaag agcacggcgt gaccatcacc
     9721 tt
//
