LOCUS       NZ_CP070373            10519 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8034225
            Orig. end                         :: 8044744
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10519
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="35"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10519
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10519
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5519](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5519)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            247..525
                     /locus_tag="GWQ54_RS38260"
     CDS             247..525
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS38260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167159077.1"
                     /transl_table=11
                     /translation="MNVRRYLAAAAAGTALAAVIAPSAHAFDLGGTLSGAARTTSAAGA
                     QIKPAANSVVGDKVGQKVTAVKDGVKAGGEAVKAANDAVKAGHALVG"
     gene            600..1880
                     /locus_tag="GWQ54_RS35375"
     CDS             600..1880
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845752.1"
                     /locus_tag="GWQ54_RS35375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_167159075.1"
                     /sec_met_domain="p450 (E-value: 1.5e-17, bitscore: 62.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPRTVPAPRGATGDLADPAFWKQPPAQRLARFAELRQQPRPVF
                     FTERRAGRRRPEQGFYALVRHADVLKASRLPRVFASAPGVTSPEPARWVRAVFGDSMVN
                     LDGADHTRLRRIVQQAFTPRLLAATEVSIRELAVRIVDDMVAERPDEFVSAVASRMSFE
                     VICAMLGVPDAYRRRIARQIDRASEHAGVRRSIGARLLSPGKGLRALARMQLTVARLGR
                     ERRRNPTGDLISALVCADVDGQRLTYRQLGSFFSLLLVAGVETTRNAIAHALSLLTDHP
                     DQLALLRGDFERYADGAVDEIVRHSTPIIQFRRTVTSRYVLSGHTFLPGDKVMLLYASA
                     NRDADVFTDPDAFDITRDPNPHLGYGGGGPHYCLGAHLARLEIKAVLRELLTRPIAVRT
                     LGEPELGTSNFDHRVRRQPFVFDAPRT"
     gene            1930..2163
                     /locus_tag="GWQ54_RS35380"
     CDS             1930..2163
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS35380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167159073.1"
                     /transl_table=11
                     /translation="MNTHHRLKAATVAASLLLLAAPGIAAADDGVAGQGSVSEFGQPKV
                     LGGRGKIGETLTSGVATLSGALQRGGSPSAGH"
     gene            2343..>3167
                     /locus_tag="GWQ54_RS35385"
                     /pseudo=""
     CDS             2343..>3167
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990359.1"
                     /locus_tag="GWQ54_RS35385"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyl hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFTGSDGAGVGRLAAAEKWRGRGPLRVGHTYLPGGTWNDIEGPPG
                     LLAPWARWRDQRTDRVLVLNVPMLDHNEEGVPDARVAALLGQASRGAFDAHFRTLAERL
                     VALRLADTIVVLGWEMNGVTYTHRCGPDPAAWRTYWRRVVTAMRSVTGQRFRFDFAPSR
                     GRDAVPWTACYPGDAWVDVLGMDAYDQPAGMSFAQQVEEPYGLRAQVDFAARHHKPVSY
                     PEWGLFRNGDDPAYVRGMTAWFRRYRPVYQTLTDYCPHGLWECGTNPRAARAY"
     misc_feature    3317..3332
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.15"
                     /tool="antismash"
     gene            3383..3634
                     /locus_tag="GWQ54_RS35390"
     CDS             3383..3634
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS35390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167159071.1"
                     /transl_table=11
                     /translation="MSKFSRFSGPAARAALVAALVSVLGTGYAAHAVDHPAPAPPQLAH
                     AGADSRASLLGALSVGAVVTGTAAVALSRRVSRSRYGK"
     misc_feature    complement(3711..3727)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 18.42"
                     /tool="antismash"
     gene            3770..4039
                     /locus_tag="GWQ54_RS35395"
     CDS             3770..4039
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818707.1"
                     /locus_tag="GWQ54_RS35395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_100573945.1"
                     /transl_table=11
                     /translation="MSRIAKTAAVVAGAGAMALAGAGIASADAGAEAVAANSPGVLSGN
                     LLQVPVHVPVNLCGNSVNVIGLLNPAFGNTCVNADSHHEGGYGE"
     gene            complement(4145..5017)
                     /locus_tag="GWQ54_RS35400"
     CDS             complement(4145..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="GWQ54_RS35400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_167159069.1"
                     /transl_table=11
                     /translation="MVYTRKNQRNLTSAERKAFVNAVLELKRSGVYDEFVRTHIEFYVP
                     DGDEGLRVAHMTPSFLPWHRQFLLEFEQALHKVNSRVTVPYWDWTRDRSPASSLWSDDF
                     MGGNGRRGDLQVMTGPFAYAHGNWDLAVEVTEARFLTRDLGRPNDPIALPVAADVDRAL
                     ADPVYDTAPWDSTSGSGLRNRMEGWTTGLSGSARFRMHNRVHRWVSGVMLGAASVNDPV
                     FWLHHAYIDSLWARWQAAHPKSQRYLPATPPPSGDPQHGRVAALHDPMPPWNVAPAALL
                     DHTAIYRYA"
     gene            complement(5001..5519)
                     /locus_tag="GWQ54_RS35405"
     CDS             complement(5001..5519)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512960.1"
                     /locus_tag="GWQ54_RS35405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_167159067.1"
                     /sec_met_domain="melC (E-value: 3.3e-37, bitscore: 126.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDATHFWRISRAGRTRPRGRAPEPAGPASRPAWQPARRGLLRAA
                     FVSATVAFTAAVLSRIPGATPPGGPAGALGPEPDAGAAFDEMHAGHRIQGWPLGAGRGE
                     LAVFVDGRQLDVMRRADGSYLSSVDHYVSYPTARAAARAAANELGTARLAAVPVHGSHQ
                     EGAGRGVHA"
     gene            complement(5828..6478)
                     /locus_tag="GWQ54_RS35410"
     CDS             complement(5828..6478)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381769.1"
                     /locus_tag="GWQ54_RS35410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_167159065.1"
                     /transl_table=11
                     /translation="MPLPGTIPQQAPATDSSGPPPAATAAFGAGRGFAWLVVLAGAAGL
                     LASWVITLDEFELLRNPNFVPGCSLNPVISCGNIMKSAQASVFGFPNPMLGLVAYGIVG
                     CVGMSLLGGGRFGRWYWRTLNAGALAGVVFVGWLQFESLYRINALCLWCCLAWVATIAL
                     FWGVTGLNIVKGALPAPSWARSLFAEFGWAVPVLHIGVIGMLILTRWWDFWTG"
     gene            complement(6518..7018)
                     /locus_tag="GWQ54_RS35415"
     CDS             complement(6518..7018)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003505.1"
                     /locus_tag="GWQ54_RS35415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5949 family protein"
                     /protein_id="WP_161255507.1"
                     /transl_table=11
                     /translation="MTSATSAVQPSRAALLGSLIVIAWSGESPDGSGDVPFLMAYSLGD
                     GAEGPEGAEAAARSLLDEIGMPAGSVVDQSQGGSFPVTLIVQGGQAVLTMPTLNAQCPV
                     PPEWLAAAEARGTVQFMFATRSWPEGVPGVAVSDEQLKAFAGDEDTISTAGHCVLPVRR
                     LRG"
     gene            complement(7233..7475)
                     /locus_tag="GWQ54_RS35420"
     CDS             complement(7233..7475)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743016.1"
                     /locus_tag="GWQ54_RS35420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_167159063.1"
                     /transl_table=11
                     /translation="MNTAKKTALALAAAGLATGAASGAAFADAGAQGVAANSPGVLSGN
                     MAQVPVHVPVNLCGNTVDVIGGLNPAFGNTCVNSD"
     misc_feature    7527..7542
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 24.66"
                     /tool="antismash"
     gene            complement(7634..7939)
                     /locus_tag="GWQ54_RS35425"
     CDS             complement(7634..7939)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035834.1"
                     /locus_tag="GWQ54_RS35425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167159061.1"
                     /transl_table=11
                     /translation="MKSMKVAAALAGSLAVVGAAAPAFAAGDLPPMSLNGGLDTIAQNV
                     SSAPNMIDASPVRTNLLDPKSDDSLLHTVGATAHDLNKTNGAPNPGQLIGGMPLGG"
     gene            complement(8200..8829)
                     /locus_tag="GWQ54_RS35430"
     CDS             complement(8200..8829)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660307.1"
                     /locus_tag="GWQ54_RS35430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167159059.1"
                     /transl_table=11
                     /translation="MLVRTPRRLVAAALSTVIVAGSAGTAMAADSPPPRTVRAEAPVPG
                     ADALLGQAQQLNGLGGVLTPVTDLLTGVLKGGSAADTAALAGKATSALDALKQAAPAAP
                     VTPPAAAPVTPPAAGPAAVPLGGPEQDAVLAAAKDDAVAVVKKGIDDLVKAVTSVNVGA
                     IVPAATSVVTGLVGLVLTTVTGGGLPVPNLPGLPKAPTDAVPKLPV"
     misc_feature    8914..8929
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.07"
                     /tool="antismash"
     gene            complement(9056..9295)
                     /locus_tag="GWQ54_RS35435"
     CDS             complement(9056..9295)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="GWQ54_RS35435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_100573938.1"
                     /transl_table=11
                     /translation="MSRTAKALVLSTAAVAAIAGGAGIAAADAGAHGAATNSPGVLSGN
                     MAQVPVHIPVNVCGNTVNVIGLLNPAFGNTCINK"
     misc_feature    complement(9305..9319)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.76"
                     /tool="antismash"
     gene            complement(9492..9731)
                     /locus_tag="GWQ54_RS35440"
     CDS             complement(9492..9731)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545032.1"
                     /locus_tag="GWQ54_RS35440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_167159057.1"
                     /transl_table=11
                     /translation="MNRIAKSVVSAGAGAAVVMLGAGMASADAGAQGVAANSPGVLSGN
                     LLQVPVHVPVNACGLTVNVIGLLNPNFGNTCINK"
     misc_feature    10017..10032
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.07"
                     /tool="antismash"
ORIGIN
        1 gcggccggtg ccacggcggc cgcggtcgcc atgcatgcca tgatgcccgc caggcccgcg
       61 cgtgaagtga ttcccatggg tgttctcttc cttccctggt ggcctactcg gccgtgtctt
      121 tcagcaagaa cgatcccggc ggcggtatca ctgcaaagtt cccctgcttg cagcaacgcg
      181 gcccccgtgc gcggcgcatg gaggcacggg gcgtgccctc cgcccggctc gtgcgttgta
      241 ggcggcatga acgtacgtcg atacctcgcg gccgccgcgg ccggaaccgc cctcgccgcc
      301 gtcatcgccc cctccgcgca cgccttcgac ctcggcggca ccctcagcgg tgcggcgcgc
      361 accaccagcg ccgccggtgc ccagatcaag cccgccgcga acagcgtcgt cggggacaag
      421 gtgggccaga aggtgaccgc cgtgaaggac ggcgtgaagg ccggcggcga ggcggtgaag
      481 gccgccaacg acgcggtcaa ggccggacac gccctggtcg gctgacgctt cggccgccct
      541 caactcccct gcccgacagt gttgttcgac agccgcgagg cggccaggac ggtgcgagcg
      601 tgactcaccc ccggacggtc ccggcgcccc gcggcgccac cggcgatctc gccgacccgg
      661 cgttctggaa gcagcctccc gcccaacgcc tcgcgcgttt cgccgagttg agacagcagc
      721 cgcgcccggt cttcttcacg gaacgccggg ccggccggcg ccgcccagag cagggcttct
      781 acgccctggt ccggcacgcc gacgtgctga aggcgagccg ccttccgcgg gtcttcgcga
      841 gcgcgcccgg cgtcacttcg cccgagccgg cccgctgggt gcgggccgtg ttcggcgact
      901 cgatggtcaa cctcgacggc gccgaccaca cgcgcctgag gcgcatcgtg cagcaggcgt
      961 tcaccccgcg cctgctcgcc gccaccgagg tcagcatccg cgagctcgcc gtgcgcatcg
     1021 tggacgacat ggtcgccgag cgccccgacg agttcgtctc cgcggtcgcc tcccggatgt
     1081 cgttcgaggt catctgcgcc atgctcggcg tgcccgacgc ctaccgccgc cggatcgccc
     1141 gccagatcga ccgggcatcc gagcacgccg gggtgcggcg cagcatcggc gcccggctgc
     1201 tcagccccgg aaagggactg cgggcgctgg cccggatgca gctgaccgtg gcccgtctcg
     1261 ggcgggagcg gcgccggaac ccgaccggcg acctcatctc ggcgctggtg tgcgcggacg
     1321 tggacggcca gcgcctgacg taccgtcaac tggggtcgtt tttctccctg ttgctcgtgg
     1381 caggagtcga gacgacccgc aacgccatcg cccacgccct gtccctgctg accgaccacc
     1441 cggatcaact ggccttgctg cgaggggatt tcgagcgcta cgcggacggc gcggtcgacg
     1501 agatcgtgcg gcactccaca cccatcatcc agttccgccg gaccgtcacc tcgcgctacg
     1561 tcctgtccgg ccacaccttc ctgcccggcg acaaggtgat gctcctgtac gcgtcggcca
     1621 accgggacgc cgacgtcttc accgacccgg acgccttcga catcacccgc gaccccaacc
     1681 cgcacctggg ctacgggggc ggcggcccgc actactgcct gggcgcgcat ctggcccgcc
     1741 tggagatcaa ggccgtactg cgcgagctgc tgacccgtcc gatcgccgta cgcacgctcg
     1801 gggaaccgga gttgggcacg tccaacttcg accaccgggt gcgcaggcag cccttcgtct
     1861 tcgacgcgcc caggacgtga ccgcccgacc gccgcgcacc ctcaccgggc gagcggcgtt
     1921 gtaccgggca tgaacactca ccaccgcctc aaggccgcca ccgtcgccgc gtccctcctg
     1981 ctcctggccg cgcccggcat cgccgcggcc gacgacgggg tcgccgggca gggcagtgtc
     2041 agcgagttcg ggcagcccaa ggtcctcggc gggcgcggca agatcggcga gacgctgacc
     2101 tcgggtgtcg ccacgctgag cggggcgctc cagcgcggtg gctccccgtc cgcgggccac
     2161 tgacggccag gacccgccgc agcgccgctc cccggggccc cgtgcacccg tccggcgccg
     2221 gcccggccgc gtggaagtgc gcgctgtgcg tactggtcct gctgctctcg gccctcgtgc
     2281 cgctcgcgtc cgacagcggg cggccgggcc cggcgcctcc cccgtcgggc aacgcggcga
     2341 gcatcttcac gggctcggac ggggccggcg tcggcaggct cgcggcggcc gagaaatggc
     2401 gcggccgggg gccgctgcgc gtgggccaca cctacctgcc gggcggcacc tggaacgaca
     2461 tcgaggggcc gccggggctg ctcgccccct gggcgcgctg gcgcgatcag cgcacggacc
     2521 gcgtcctcgt cctcaacgtg ccgatgctcg accacaacga ggagggcgtg cccgacgcgc
     2581 gggtagcggc cctgctcggc caggcctcgc ggggcgcctt cgacgcccac ttccggaccc
     2641 tggccgagcg tctggtcgcc cttcgactcg ccgacacgat cgtggtgttg ggctgggaga
     2701 tgaacggcgt cacctacacc caccggtgcg gccccgatcc cgccgcgtgg cggacgtact
     2761 ggcgccgcgt cgtgacggcg atgcggtcgg tcacggggca gcggttccgc ttcgacttcg
     2821 cgccgagccg ggggcgggac gcggtgccgt ggaccgcgtg ctacccgggg gacgcctggg
     2881 tggacgtcct cggcatggac gcctacgacc agcccgcggg catgtcgttc gcccagcagg
     2941 tcgaggagcc gtacgggctg cgggcccagg tcgacttcgc cgcccgccac cacaagccgg
     3001 tctcgtaccc ggagtggggc ctcttccgca acggggacga cccggcgtac gtgcgcggga
     3061 tgaccgcctg gttccggcgg taccggccgg tctaccagac cctgaccgac tactgcccgc
     3121 acggcctgtg ggagtgcggc acgaatccgc gggcggcccg ggcctatctc ggcgcccggt
     3181 gaacggcccg gcgtccgccc tcggggacgg cccgcgcact gggccgtgcg ggggccgggg
     3241 gctgcgccgg acgggttgtc tgtcggctgc ggcgcgcgtg gccgggggac cggggcgggg
     3301 ccgatccgac ccaatgaatt acgcgtatca acatctgatg cgcctccccc ctcccccgtc
     3361 ttcctgtcga agggtcgcag gcatgtccaa gttctcgagg ttctcgggtc ccgcggcgcg
     3421 cgccgccctc gtcgcggctc tggtctccgt gctcggcacg gggtacgcgg cgcacgccgt
     3481 cgaccacccc gccccggccc cgccgcaact cgcccacgcc ggcgccgact cccgggcctc
     3541 gctgctcggc gcgctgagcg tcggcgcggt cgtcacgggc acggcggcgg tcgccctctc
     3601 gcggcgcgtc tcccgcagcc gctacggcaa gtagcggcag tccttgcgcc ggtcggccgt
     3661 gcagaaccgt tttgggcgca ggtgtccgtt acttatgccc gttcggcacg ttgaagtgaa
     3721 atgacgactg cgtgtctgcg gtcgcgtgtc catatcaagg agaactttga tgtctcgtat
     3781 cgcgaagacg gccgccgtcg tcgccggagc aggcgccatg gcgctggccg gtgcgggtat
     3841 cgcctcggcc gacgccggcg ccgaggccgt cgctgcgaac tcccccggcg tcctgtccgg
     3901 caacctgctc caggtgccgg tgcacgtccc ggtcaacctc tgcggcaact cggtcaacgt
     3961 catcgggctg ctgaaccccg cgttcggcaa cacctgcgtc aacgccgaca gccaccacga
     4021 gggcggctac ggggagtaag taccccccgc ttcggccggg ctccctcacg gtggcccggc
     4081 cggtcatggc tcacagcagt accgcggcct cccggacttc tccccccggg aggccgtcgc
     4141 cgtgtcaggc gtaccggtag atcgcggtgt ggtcgagcag tgccgccggt gccacgttcc
     4201 acggcggcat cgggtcgtgg agggccgcca cccggccgtg ctgggggtcc ccggacggcg
     4261 gtggcgtggc cggcagatag cgctgggact tggggtgcgc ggcctgccag cgggcccaca
     4321 ggctgtcgat gtaggcgtgg tgcaaccaga acaccggatc gttgacggag gcggcgccca
     4381 gcatgacgcc gctgacccag cggtgcaccc ggttgtgcat gcggaagcgg gcgctgcccg
     4441 aaagcccggt cgtccagccc tccatgcggt tgcggagacc ggacccgctg gtggagtccc
     4501 agggcgcggt gtcgtacacc gggtcggcga gtgcccggtc gacgtcggcg gcgacgggca
     4561 gggcgatggg gtcgttgggg cgccccaggt cccgggtcag gaagcgggcc tcggtgacct
     4621 cgaccgcgag gtcccagttg ccgtgcgcgt acgcgaacgg cccggtcatg acctgcaggt
     4681 cgccgcggcg gccgttgccg cccatgaagt cgtcggacca gagggacgac gcgggtgaac
     4741 ggtcccgcgt ccagtcccaa taaggcacgg tcacacggga gttgaccttg tgcagggcct
     4801 gctcgaattc gagcaggaac tggcggtgcc agggcaggaa cgacggcgtc atgtgcgcga
     4861 cccgcagccc ttcgtcgccg tccgggacgt agaactcgat gtgggtgcgc acgaactcgt
     4921 cgtagacacc gctgcgtttg agttcgagca ccgcgttcac gaaggccttg cgctcggcgc
     4981 tcgtcaggtt ccgctggttc ttacgcgtgt acaccacggc cggctccctc ctggtggctg
     5041 ccgtgcacgg gtacggcggc gaggcgggcg gtgcccagct cgttcgcggc ggcccgggcg
     5101 gccgcgcgtg cggtggggta cgacacgtag tggtcgacgg agctgaggta gctgccgtcc
     5161 gcccgccgca tcacgtccag ctgccggccg tccacgaaca cggcgagctc gccccggccc
     5221 gccccgagcg gccagccctg gatgcgatgt ccggcgtgca tctcgtcgaa ggcggcgccc
     5281 gcatcgggtt ccggcccaag ggcacccgcg ggtccgcccg gcggggtggc gcccgggatg
     5341 cgggagagga cggcggcggt gaaggcgacg gtcgcggaga cgaacgcggc ccgcaacagg
     5401 ccccgccggg ccggctgcca ggcggggcgg gacgcgggcc cggcgggttc gggggctcgc
     5461 ccccgtgggc gggtgcggcc cgcccggctg atgcgccaga agtgcgtcgc gtcgttcatc
     5521 ggcgtctccc tcgtgctcgc cggggcggac gcgtccggca cacacgtggg ccccggaaac
     5581 cgtgctggtt ccggggcccg tccaaacgtc cgctccttgt ccgatgccgg gttcaacgag
     5641 gcccggcgtc ccaggaaacg gccacggccg tcggcgcgcg gcagcgggcg ctccccgggc
     5701 cggtactgga tgcacggccc ggggaggcct caaccatgcg actggtcagt gggtcaacgc
     5761 cgccgggcgc ccgccgtggc ggcgggggcg ggcgaatcac gcggtcggac caacgttccc
     5821 gcggggctca gcccgtccag aagtcccacc agcgggtgag gatcagcatc ccgatgaccc
     5881 cgatgtgcag caccggcacc gcccagccga actcggcgaa caggcttcgg gcccaactcg
     5941 gcgcgggcag agcccccttg acgatgttga ggccggtcac cccccagaac agggcgatgg
     6001 tggcgaccca cgccaggcag caccacaggc acagcgcgtt gatccggtac agcgactcga
     6061 actgcagcca gccgacgaag acgaccccgg ccagcgcccc cgcgttgagc gtgcgccagt
     6121 accagcgccc gaaccggccg cccccgagca ggctcatgcc cacgcagccg acgatcccgt
     6181 acgcgaccag gccgagcatc gggttgggga aaccgaagac ggaagcctgg gcgctcttca
     6241 tgatgttgcc gcaggagatc accgggttga ggctgcaccc ggggacgaag ttggggttcc
     6301 gcagcagctc gaactcgtcg agggtgatga cccacgacgc gagcagcccc gccgcgccgg
     6361 caaggacgac cagccaggcg aagccccgcc cggcgccgaa cgcggcggtg gccgcgggtg
     6421 gggggccgga cgagtcggtg gcgggagcct gctgcgggat cgtcccggga aggggcatgg
     6481 ccttacgtct ccgttctcgt ccggcggtgg ggtgcggtca gccgcgcagg cggcggacgg
     6541 ggagcacgca gtggccggcg gtggagatgg tgtcctcgtc gccggcgaac gccttcagct
     6601 gctcgtcgga gacggcgact ccgggcacgc cctcgggcca ggagcgggtg gcgaacatga
     6661 actggacggt gccccgcgcc tcggcggcgg ccagccactc gggcggcacc gggcactggg
     6721 cgttgagcgt cggcatggtg aggacggcct ggccgccctg gacgatgagg gtcaccggga
     6781 acgagccgcc ctgcgactgg tcgacgacgc tgccggccgg catgccgatc tcgtcgagca
     6841 gcgaccgcgc cgcggcctcc gcaccctcgg ggccctcggc cccgtcaccg agggagtacg
     6901 ccatgaggaa gggcacatcg ccggacccgt cgggggactc accgctccac gcgatgacga
     6961 tgagcgagcc caacagggct gccctggaag gctgtacggc actggtggct gaagtcatgc
     7021 cgggactcta ggacattccg ggccgagccc ttcgcagctg tcacccatcc ggatgaaagg
     7081 tccttcaccg gtcttctggc acccgtgtac gactcttcgg tcacccgtgc gcgactcggc
     7141 gcgtccggac acggcgaagc ggctccggga tccccaactc cacgaagccg ctcaccacgc
     7201 gctgaagccc gccggtctca accggcccta cttcagtccg agttgacgca ggtgttgccg
     7261 aaggccgggt tcagaccgcc gatgacgtcg accgtgttgc cgcagaggtt caccgggacg
     7321 tggaccggga cctgcgccat gttgccggac aggacgccgg gcgagttcgc ggcgacaccc
     7381 tgcgcgcccg cgtcggcgaa ggcggcgccg gaggcggcac ccgtggcgag cccggcggcg
     7441 gcgagggcga gagccgtctt cttggcagtg ttcatgatgg gaccttccat tcgggggttg
     7501 cctcagcgta ggcaaatggc cgggagccgc cggaagcgaa cacgccgggt ttattgtctt
     7561 tccctcgtac agcgcacgag gcggccggac ggcccctcaa ttcacgctgt ggaaggactc
     7621 gttcgcgcct gcgctaaccg cccagcggca ttccgccgat gagctgtccc ggattgggtg
     7681 ccccattggt cttgttcagg tcgtgcgcgg tggcgcccac ggtgtggagc agggagtcgt
     7741 ccgacttggg gtcgagcagg ttggtgcgca ccggcgacgc gtcgatcatg ttgggagcgg
     7801 acgacacgtt ctgggcgatc gtgtcgaggc cgccgttcag gctcatgggc gggaggtccc
     7861 cggccgcgaa ggccggtgcc gccgcaccca cgaccgcgag ggatccggcg agtgccgcgg
     7921 caaccttcat cgacttcatc atcaatcctt ccgcggcgag ttccgtcgcc gaaccgtgct
     7981 tgtccctgaa tgcattgcgc tgcttcgccc tgggtaacga cccgccgacg cccgggaaac
     8041 ccttcgattc cggattcatg ggccgccacc caattgcggg gcccgccgat acgcacgtgg
     8101 gatttcatga attctgctgt gcggtaagga ggttgatggg tcgggacagc cgagagcgcc
     8161 ggtcccgtcc gtgacgggcc ggcgctctcg gggtgcggct cagacgggca gcttcggcac
     8221 cgcgtccgtg ggcgccttgg gcagccccgg caggttgggc accggcaggc cgccgccggt
     8281 cacggtggtc aggacgagcc cgacgagacc ggtgaccacc gaggtcgcgg cggggacgat
     8341 ggccccgaca ttgacactgg tgaccgcctt gacgaggtcg tcgatgccct tcttgaccac
     8401 ggcgacggca tcgtccttgg cggccgccag cacggcgtcc tgctcggggc cgcccagcgg
     8461 cacggccgcc ggaccggcgg cgggcggggt gaccggcgcg gcggccgggg gcgtgacggg
     8521 ggccgccgga gccgcctgct tcagcgcgtc gagggcggac gtggccttgc cggccagcgc
     8581 cgcggtgtcg gctgccgagc ctcccttgag cacaccggtc agcaggtcgg tcacgggcgt
     8641 gaggacgccg ccgagaccgt tcaactgctg ggcctggccc agcagggcgt cggcgcccgg
     8701 gaccggcgcc tcggcacgga ccgtacgcgg cggcggcgag tcggcggcca tcgcggtccc
     8761 ggcggacccg gcgacgatca cggtggaaag ggccgctgcg accaggcgcc gcggtgttcg
     8821 tacaagcatg aaacggttct ccttgggtgg ggcgagcgag ctctcctcac cgtgcgcacg
     8881 cccccaaccc ctcgcaaccg gatggtcgtc ctgagtgatc atcagttgcc cgccacgccg
     8941 cgccgagctg gcacaacccc gtaccggaag agcccggcgg actaccgcca tccggggcag
     9001 cacgaagccc cggcccgctc cctggagagg agcggaccgg ggcttcgacg aggaactact
     9061 tgttgatgca ggtgttgccg aacgcggggt tcagcagacc gatgacgttc acggtgttgc
     9121 cgcagacgtt gaccgggatg tgaacgggga cctgcgccat gttgccggac aggacgccgg
     9181 gggagttggt ggccgcgccg tgcgcaccgg cgtcggcggc ggcgatgccg gccccaccgg
     9241 cgatggcggc aacggcagcg gtggacagga ccagggcctt cgcggtacgc gacatggaga
     9301 agcactccta gattgagaaa gtcattgacc gggtgtttgt cactcgacct acacctcaac
     9361 gcgaacccgg ccgcccggtt ttgcccctaa cgggtgaacc agggttttgg gggccgcgaa
     9421 aatggcgagg gggcccgtcc ggaccgaggc cgcgagccgc ggcgccggtc cggcggcgtc
     9481 acccctgggg attacttgtt gatgcaggtg ttgccgaagt tcgggttcag cagaccgatc
     9541 acgttcacgg tcaggccgca ggcgttgacc gggacgtgca cggggacctg gagcaggttg
     9601 cccgacagga cgccggggga gttggcggcg acgccctgcg cacccgcgtc ggcgctggcc
     9661 atgcccgcac cgagcatcac gaccgcggca cctgcgcccg ccgacacaac agactttgca
     9721 attcgattca tcgctttttc ctttcgggtg gagatggggc caccgataca acgaccgtgt
     9781 cgtcaagggg atacgcacga tcgctcgaag gggacactcc gtctccacat tcatgaccgg
     9841 accgggttgt aaatacggtt acagttgccc accgccgggc cgcgccacat acgggatctt
     9901 cggcggccgg agcgccggcc ctgcggaatt cgagggcggc cgcactgcgg ggagagcccc
     9961 gcagcatttt tgttcagctt tcttttcgag cgccaggacc ggagtgaccg cgcatgccga
    10021 cgagagcgaa cacccccggc ggacccgagg ggccggggtc cgccgggcag ttgagtgtcc
    10081 tgcacctggt tcagcccgtt gaggggggcg tagcacgagt cgtcacggac ttggtgcggg
    10141 accagctgcg cagtggattc cgggtcaccg tggcatgccc cgagggaggc ccgttgagca
    10201 ccgaactgac gcgtctgggt gcccgggtgc acccctggcg ggccacccgt tcacctggcc
    10261 ccgcgctcct cggggagacc ctgcgggcgg cccgcctcgt ccggcgggtg cgccccgacg
    10321 tgatccacgc ccacagcgcc aaggccgggg tcgccgcccg gctcgccgtg cgcggcaggg
    10381 tgcccaccgt cttccagccg cacgcctggt cgttcgaggc cgtcggcggc atcaccgcgc
    10441 ggctctcccg aggttgggag cgtgccgcgg tgcgctggac cgcgcggatc gtgtgcgtca
    10501 gcgagggcga gcgggccac
//
