LOCUS       NZ_CP070373            10405 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6339708
            Orig. end                         :: 6350113
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="33"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(136..149)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.11"
                     /tool="antismash"
     misc_feature    complement(166..176)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(518..1603)
                     /locus_tag="GWQ54_RS28065"
     CDS             complement(518..1603)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="GWQ54_RS28065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_167154135.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.7e-19, bitscore:
                     68.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRTVLHVKGRVLAGPEDVRDELWAVDGRITYERPPGEALTVSG
                     WALPGLVDAHCHVGLDQHGPVSDEVSEKQALTDRDAGTLLVRDAGSPSDTRWIDAREDL
                     PKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRDAGDLA
                     ACWPRGEVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTDDTVPLFAE
                     RGVAIVPTLVNIATFPHMADGGEAKFPDWAAHMRRLYERRYDTVRSAYDAGVPVFVGTD
                     AGGSLAHGLVAAEVAELVKAGIPALDALSATAWGARAWLGRPALEEGAPADLVVYDQDP
                     RADVRVLAAPSRIVLNGRIVG"
     gene            1720..2817
                     /locus_tag="GWQ54_RS28070"
     CDS             1720..2817
                     /NRPS_PKS="Domain: Aminotran_5 (16-310). E-value: 3.7e-29.
                     Score: 93.7. Matches aSDomain:
                     nrpspksdomains_GWQ54_RS28070_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="GWQ54_RS28070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_167154133.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.6e-22, bitscore:
                     78.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLAPLTADHFAAIERRVAALLATEQDVVIMQGEALLPLE
                     GCIRGAARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLEVPFHAAVTAEQIDRALTDR
                     PEIDFVSLVHAEAATGNTNPVAEIGDVVRRHGALFMLDAVASVAAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPSAPRRSYLSLLDWKQRWIDGGRKALLHAP
                     AQLEMLALEACVERIESEGLDAVMARHASAAAATRAGALGLGGGLVPYVHEAADAAPVA
                     TTLRTPEGVDASELVARALAADPSLPLIAGGGALASTMVRVNHYGVDATRGVVQSSLAA
                     LGAALGEYGIAVDLEAARKAVSTTW"
     aSDomain        1768..2649
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GWQ54_RS28070_Aminotran_5.1"
                     /evalue="3.70E-29"
                     /label="GWQ54_RS28070_Aminotran_5.1"
                     /locus_tag="GWQ54_RS28070"
                     /protein_end="310"
                     /protein_start="16"
                     /score="93.7"
                     /tool="antismash"
                     /translation="AAIERRVAALLATEQDVVIMQGEALLPLEGCIRGAARPGSTALNV
                     VTGPYGQTFGNWLRDCGAEVVDLEVPFHAAVTAEQIDRALTDRPEIDFVSLVHAEAATG
                     NTNPVAEIGDVVRRHGALFMLDAVASVAAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAV
                     SVSARAWERIAANPSAPRRSYLSLLDWKQRWIDGGRKALLHAPAQLEMLALEACVERIE
                     SEGLDAVMARHASAAAATRAGALGLGGGLVPYVHEAADAAPVATTLRTPEGVDASELVA
                     RALAADPSLPLIA"
     misc_feature    2921..2930
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    2993..3008
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3018..3032
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.75"
                     /tool="antismash"
     misc_feature    complement(3029..3043)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.85"
                     /tool="antismash"
     misc_feature    complement(3103..3114)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.18"
                     /tool="antismash"
     gene            3142..3663
                     /gene="ectA"
                     /locus_tag="GWQ54_RS28075"
     CDS             3142..3663
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="GWQ54_RS28075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_161247033.1"
                     /transl_table=11
                     /translation="MTAAQTAVRIESPRVEDGAAIWRIARDSEVLDLNSSYSYLLWCRD
                     FARTSAVARDDDGTPIAFITGYVRPDRPGTLVVWQVAVDHAHRGHGLAGLLLEALTARV
                     SASHGTTCLETTVSPDNTPSDRLFTSFAKRRGAALDRELLFDAGLFPDDGHQPEVLYRI
                     GPLHPQGETL"
     gene            3660..4931
                     /gene="ectB"
                     /locus_tag="GWQ54_RS28080"
     CDS             3660..4931
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 4.1e-71.
                     Score: 231.7. Matches aSDomain:
                     nrpspksdomains_GWQ54_RS28080_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848583.1"
                     /locus_tag="GWQ54_RS28080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_167154131.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.4e-76, bitscore:
                     254.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRGWPAVFDRAQGARITDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALLDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGRVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCRRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKGELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADAGQMEKHTLARGEQIEQAL
                     LALCTEHSRLGASYRGRGLVWGLEFADPARASEVCARAFELGLLLETSGPKSEVVKLLP
                     SLTITHEELDEGLRSLARAVAATV"
     aSDomain        3744..4757
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GWQ54_RS28080_Aminotran_3.1"
                     /evalue="4.10E-71"
                     /label="GWQ54_RS28080_Aminotran_3.1"
                     /locus_tag="GWQ54_RS28080"
                     /protein_end="366"
                     /protein_start="28"
                     /score="231.7"
                     /tool="antismash"
                     /translation="FDRAQGARITDEDGHSYLDFFAGAGSLNYGHNNPVLKRALLDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGRVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCRRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADAGQMEKHTLARGEQIEQALLALCTEHSRLGASYRGRGLVWGLEFA"
     gene            5001..5405
                     /locus_tag="GWQ54_RS28085"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="GWQ54_RS28085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_100574374.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.9e-54, bitscore:
                     181.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNEETGETHWIEPGTMYLLNGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEEG"
     gene            5408..6268
                     /gene="thpD"
                     /locus_tag="GWQ54_RS28090"
     CDS             5408..6268
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885284.1"
                     /locus_tag="GWQ54_RS28090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_167154129.1"
                     /sec_met_domain="PhyH (E-value: 6.3e-40, bitscore: 136.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQDLYPTRGSAEVVTPRQDPVVWGPSAGLESFEQDGFLAIEDL
                     LSDDEVALYRSELERLIADPAVRADERSIIEPQSQSVRSVFEVHRISEIFARLVRDERV
                     VGTARRILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMRTVSVSIALTE
                     NHDTNGGLMIMPGSHRDFLGCEGATPKDNYKKSLQMQDAGTPSDEALTKMADRHGIKLF
                     TGRAGSATWFDCNAMHGSGDNITPFPRSNVFIVFNSVENAAEDPFAAPIRRPEFIGARD
                     FTPVR"
     gene            6384..7052
                     /locus_tag="GWQ54_RS28095"
     CDS             6384..7052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140978.1"
                     /locus_tag="GWQ54_RS28095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167154127.1"
                     /transl_table=11
                     /translation="MSMVDEPSRTPVTAADVEHAVRLSVTALRAGLAADWSAPAGTLEW
                     DCWETVEHLSDDLFAYAVQIGPAQPPLDGEVPYLWEARRPGGPRNAVHADRAAGPAGLL
                     QTLEASGALLTAMVRTASPEVRAYHGFGVSDPEGFGAMGIVETLLHTHDVAEGLGLAWT
                     PPTGLCDRVLARLFRDVLEDPDRWRVLLWATGRADLAGRTRPDKWRWYGEPLSSGAATP
                     "
     gene            complement(7027..7728)
                     /locus_tag="GWQ54_RS28100"
     CDS             complement(7027..7728)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151645.1"
                     /locus_tag="GWQ54_RS28100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167156357.1"
                     /transl_table=11
                     /translation="MESDTTGQAREAARNNADWCAALCRAHGITGREGPYAWTSPRRTP
                     LYYPDAVTLTPEASPGDVLGSIDLTAPGASVKDSFAVLDLSDDGFEVLFEAQWIHRAAP
                     GITGKGWQLVHGEGELAAWERAWSDGGSEGLFPPALLAEPGHSFLVERDERGEATAGAV
                     ASRSGDVVGISNLYAVDGDQDAAWAGCLATVARLWPGREVVGYESGDDLAAALRAGFAP
                     AGPLRVWLRPN"
     gene            complement(7778..8869)
                     /locus_tag="GWQ54_RS28105"
     CDS             complement(7778..8869)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643609.1"
                     /locus_tag="GWQ54_RS28105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkene reductase"
                     /protein_id="WP_167154125.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTPLANRIALAPMTRSRATGPGNSPTASTAEYYA
                     QRASAGLLITEGVQPSPVGQGYPDTPGLHSDEQIAAWRKVTDAVHAKGGRIFAQLMHAG
                     RIGHPDLLPGDLINVGPSAVAADGQVFTHEGPKPFITPQALSEDQVLVTIGDFAAAARN
                     AIEAGFDGVELHGANGYLIHQFLAPNSNVRTDGWGGSVEGRIRFAVEVTKAVAAEIGAE
                     RTAIRLSPGNPYNGIVEPEPDPVYTALVAALEPLGLAYLHVLEVAPESRELTLRLRKSF
                     SGVFILNAATEGPTGPDALTLIEDGTADLISYGALFLANPDLPARLRAGGPFNAPDPST
                     FFGGDDRGYTDYPALDAAAPLGA"
     gene            8993..9529
                     /locus_tag="GWQ54_RS28110"
     CDS             8993..9529
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607784.1"
                     /locus_tag="GWQ54_RS28110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_167154123.1"
                     /transl_table=11
                     /translation="MTSDEPVCTDFPHLPSAARGGPFSHAVSRVARLHRIAAGRQLRSI
                     GLYPGQELLMMHLWDAGAARQSELIKLLELDPSTVTKMLQRLEQAGYVRRSPDPKDRRA
                     ALVEATDDGCVLHERVQQAWTDLEEQTLAGLDAAERAELARLLGKVEANLCRSAGAAAD
                     CEAEPSCPSEAGCGE"
     misc_feature    complement(9596..9610)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 cccccgccgt cgccctcgac gccggtggcg tgcgcgggcg cggccagcag cagcggcccg
       61 gcgacgagcg ccgtgcaggc cgcgatcgcg gcggagcgcc ggccggccgg gacgttcgcg
      121 ggcaggcgga agttgttgct gttcaaaatg gtggaacccc cacaggagac atggcagtcc
      181 gggcgcccgc cacggggact cgtgggactc ctgcgaatcg aggggcgcgc cggcgacttg
      241 ggacaccgaa tctttacgca ctgagagtga actgaccgtc acacggcgcc agttcacccc
      301 aacgggtggt ttccgtcccc atattcgatt tgcccgcccc cagggccctc ctccgtcacc
      361 cccgcaccgc ccccgcaccc cgtacgccgc cgcaccgccc cggcgtacgc atgaagacct
      421 acaacgacct gaccgcgccg ggcgtcacgc ccggtcaacc cggtgatgcg ccgagggctt
      481 cagggaactg ccgctcccgc aggccggatc gaccgcctca gcccacgatc cgcccgttga
      541 gcacgatgcg gctcggcgcg gccaggacgc ggacgtccgc gcgggggtcc tggtcgtaga
      601 cgaccaggtc ggccggcgcg ccctcctcaa gggcggggcg gccgagccag gcgcgggcgc
      661 cccaggccgt cgccgagagc gcgtcgaggg cggggatgcc cgccttgacg agttcggcga
      721 cctcggcggc caccaggccg tgggcgagcg agccgcccgc gtccgtaccg acgaagacgg
      781 gcaccccggc gtcgtaggcc gagcgcaccg tgtcgtagcg gcgctcgtac aaccgccgca
      841 tgtgggcggc ccagtcgggg aacttcgcct cgccgccgtc ggccatgtgc gggaacgtgg
      901 cgatgttgac cagcgtcggc acgatcgcca caccgcgctc ggcgaacagc gggacggtgt
      961 cgtcggtcag gccggtggcg tgctcgatgc agtcgatgcc cgcctcgacg aggtcccgca
     1021 gcgagtcctc ggcgaagcag tgcgcggtga cgcgggcgcc gagccggtgg gcctcggcga
     1081 tcgcggcctc cacctcgccg cgcggccagc aggcggccag gtcgccggcg tcccggtcga
     1141 tccagtcgcc gaccagcttg acccagccgt ccccgcgccg cgcctcgcgc gcgacgtagg
     1201 cgaccaggtc gccgggctcg atctcgtgcg cgtagttgcg gatgtagcgg cgggtgcggg
     1261 cgatgtgccg gcccgcccgg atgatcttcg gcaggtcttc gcgggcgtcg atccagcggg
     1321 tgtcggaggg cgagcccgcg tcacgcacca gcagcgtgcc ggcgtcccgg tcggtcaggg
     1381 cctgcttctc gctcacctcg tcggacaccg gcccgtgctg gtcgaggccc acgtggcagt
     1441 gcgcgtcgac cagtcccggc agcgcccagc cgctgacggt gagcgcctcg cccggcggcc
     1501 gctcgtaggt gatccgcccg tcgaccgccc acagttcgtc ccgtacgtcc tcgggtcccg
     1561 cgaggacgcg ccccttcaca tgcagcaccg tgcgatcgct catggtcagc actgtacgag
     1621 gcgctgggta gtctccatcc ggaggatcga acacccgacc cgatcccctg cgtgacctcc
     1681 gatacacgac ctttgacccg acccgaagaa ggcatcgccg tgacacaccc gctcctggac
     1741 ctggccccgc tcaccgccga ccacttcgcg gcgatcgagc gacgggtggc cgccctgctc
     1801 gccaccgagc aggacgtggt gatcatgcag ggcgaggcgc tgctccccct ggagggctgc
     1861 atccggggcg cggcccgccc cggctccacc gcgctcaacg tggtgaccgg cccctacggg
     1921 cagaccttcg gcaactggct gcgcgactgc ggggctgagg tcgtcgacct ggaggtgccg
     1981 ttccacgcgg cggtgaccgc ggagcagatc gaccgggcgc tcacggaccg cccggagatc
     2041 gatttcgtct cgctcgtgca cgcggaggcg gcgaccggca acacgaaccc ggtcgcggaa
     2101 atcggcgacg tggtgcgccg gcacggcgcc ctgttcatgc tggacgcggt ggcctcggtg
     2161 gccgccgagc cgctgctccc ggacgcctgg ggcgtggacc tgtgcgtgat cggcgcgcag
     2221 aaggcgatgg gcggcccggc cggcgtctcg gccgtctcgg tcagcgcccg cgcctgggag
     2281 cggatcgcgg ccaacccgag cgccccgcgc cgctcctacc tctcgctcct ggactggaag
     2341 cagcgctgga tcgacggcgg ccgcaaggcc ctgctgcacg cgcccgccca gctggagatg
     2401 ctggccctgg aggcgtgcgt ggaacgcatc gagtccgagg gcctggacgc cgtgatggcc
     2461 cggcacgcct cggccgccgc ggcgacccgc gcgggcgccc tggggctggg cggcggtctc
     2521 gtcccgtacg tgcacgaggc tgccgacgcc gccccggtgg ccacgaccct gcgcactccc
     2581 gagggcgtgg acgcctccga gctggtggcc agggcgctcg ccgcggaccc ctcgctgccg
     2641 ctgatcgccg ggggcggggc gctggcctcg accatggtcc gggtcaacca ctacggcgtc
     2701 gacgccacgc ggggtgtcgt gcagtcctcg ctcgcggcac tgggcgcggc actcggcgag
     2761 tacggcatcg ccgtggacct ggaagccgcc cgcaaggccg tttccaccac gtggtgacac
     2821 tccccccgcg ttcctccggc gcgtaatgca caactgaatt ccgcaaaaca ttccacagaa
     2881 gtcccacaga acgggaagct gcttattcac cgcagcttcc cgttttctta tgccccgcct
     2941 ttccgggctc aaacgcaaag gtttcaacaa ggttccgagc ctgtcacaac ggtgtgatcg
     3001 actccacaac cccgcccttt tgtccggtaa ttaccggaca aaccggttcg cgtgcatgct
     3061 cgcgtgataa cacaaacaag ctccgagcac acccccggac gggaatgcga tttcaatttc
     3121 tggtcggtaa attccctcgg catgaccgcc gcgcaaacag cagtccgaat cgaaagccca
     3181 cgagtggagg acggagccgc gatctggcgc attgcccgtg actccgaggt gctcgacctc
     3241 aactcctcgt acagctatct gctctggtgc cgcgacttcg ccaggacctc cgcggtggcc
     3301 cgggacgacg acggcacgcc catcgcgttc atcaccggct acgtacggcc cgaccgcccg
     3361 ggcacgctgg tggtctggca ggtcgccgtc gaccacgccc accgcggcca cgggctcgcg
     3421 gggctgctgc tcgaagcgct gacggcgagg gtgtccgcct cgcacgggac gacctgtctc
     3481 gagacgacgg tctcgccgga caacaccccg tccgaccggc tcttcacctc gttcgccaag
     3541 cgccgcggcg ccgccctcga ccgagaactt ctcttcgacg cgggtctctt ccccgacgac
     3601 gggcaccagc ccgaagtcct ctaccgcatc ggccccctcc acccccaggg agaaacgctg
     3661 tgaccatcac cccgcccgcc ctgagtgtct tcgaatccct tgagtcggag gtgcgcagct
     3721 actgccgcgg ctggcccgcc gtgttcgacc gcgcgcaggg tgcccgcatc accgacgagg
     3781 acggccacag ctatctcgac ttcttcgccg gagccggctc actcaactac gggcacaaca
     3841 acccggtact gaaacgggcg ctgctcgact acatcgagcg ggacggcatc acccacggcc
     3901 tcgacatggc gacgaccgcg aaacgggcgt tcctggagac gttccagaac gtgatcctgc
     3961 gcccgcgcga cctgccgtac aaggtgatgt tcccgggccc gacgggcacc aacgccgtcg
     4021 aggcagcgct gaagctggcc cgcaaggtga agggccgcga gtcgatcgtc tccttcacca
     4081 acgccttcca cggcatgtcg ctcggctcgc tcgccgtcac cggcaacgcc ttcaagcggg
     4141 ccggggccgg catcccgctg gtgcacggca ccccgatgcc gttcgacaac tacctcgacg
     4201 gccgggtgcc cgacttcctg tggttcgagc ggctcctgga ggaccagggc tcgggcctca
     4261 acaagccggc cgccgtgatc gtggagaccg tgcagggcga gggcggcatc aacgtggccc
     4321 ggcccgagtg gctgcgcgcg ctcgccgacc tgtgccgccg ccgcgacatg ctgctcatcg
     4381 tcgacgacat ccagatgggc tgcggccgca ccggcgcctt cttctccttc gaggaggcgg
     4441 gcatcgtgcc ggacatcgtc accctctcca agtcgatcag cggctacgga ctcccgatgt
     4501 cgctctgcct gttcaagggc gagctcgacg tgtgggagcc cggcgagcac aacggcacct
     4561 tccgcggcaa caaccccgcg ttcgtcaccg cgaccgccgc gctggaggcg tactgggccg
     4621 acgccggcca gatggagaag cacaccctgg cgcgcggcga gcagatcgag caggccctgc
     4681 tcgcgctgtg caccgagcac agccggctcg gcgccagcta ccgcggccgc ggcctggtgt
     4741 ggggtctgga gttcgccgac ccggcccgcg cctccgaggt ctgcgcacgc gccttcgaac
     4801 tggggctgct cctggagacc tccggcccca agagcgaggt ggtcaagctg ctgccgtcgc
     4861 tgaccatcac gcacgaggag ctggacgagg ggctgcggtc gctggcccgc gcggtcgccg
     4921 cgaccgtctg acccgctcga ccggcagcga ccggccgtcg accggcgtac aagagataca
     4981 cagaaaggga gcaagtcacc gtgattgtcc gctcgttcaa ggacatcgag aacaccgacc
     5041 gccatgtgaa ggccgcctcc ggcacctggg agagcaagcg gatcgtcctc gcgaaggaaa
     5101 aggtcggctt ctcgctgcac gagaccgtcc tgtacgcggg caccgagacc tccatgtggt
     5161 acgccaacca catcgaggcg gtcctgtgca ccgagggcga ggccgagctc accaacgagg
     5221 agaccggcga gacccactgg atcgagccgg gcacgatgta tctcctgaac ggtcacgagc
     5281 gccacacctt gcgccccaag accgacttcc gctgtgtctg cgtcttcaac ccgcccgtca
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctgaccgaa cccgaggagg
     5401 gctgaccatg accgcccagg atctctaccc cacccgtggc tcggccgagg tggtcacacc
     5461 ccgccaggac ccggtcgtct ggggtccgtc cgccgggctc gaatcgttcg agcaggacgg
     5521 cttcctcgcg atcgaggacc tgctctcgga cgacgaggtg gcgctctacc gctcggagct
     5581 ggagcggctg atcgccgacc ccgccgtgcg tgccgatgag cggtcgatca tcgaaccgca
     5641 gtcgcagtcc gtgcgttcgg tcttcgaggt gcaccggatc agcgagatct tcgctcgtct
     5701 ggtgcgcgac gagcgggtcg tgggcacggc acgccgcatc ctcggctcgg acgtgtacgt
     5761 ccaccagtcg cggatcaacg tgaagccggg cttcggcgcc tcgggcttct actggcactc
     5821 ggacttcgag acctggcacg ccgaggacgg tctgccgcgg atgcggacgg tgtcggtctc
     5881 gatcgcgctg accgagaacc acgacaccaa cggcggcctg atgatcatgc ccggctcgca
     5941 ccgggacttc ctcggctgcg agggcgccac cccgaaggac aactacaaga agtccctcca
     6001 gatgcaggac gcgggcaccc cctccgacga ggcgctcacc aagatggccg accggcacgg
     6061 catcaagctg ttcaccggcc gggcgggctc ggcgacctgg ttcgactgca atgcgatgca
     6121 cggctccggg gacaacatca ccccgttccc gcgcagcaac gtgttcatcg tcttcaacag
     6181 cgtggagaac gcggccgagg accccttcgc ggccccgatc cgccgcccgg agttcattgg
     6241 ggcgcgggac ttcacgccgg tgcggtagcc gcaggactgc gcgcaggagc acgcgcgaag
     6301 gcaaaggccc gggggctccc ccactgctcc cgggccaggg accgtaccaa cgcctccaca
     6361 gagatcgttg gtacggtccc tccatgtcca tggtcgatga accttcccga actcccgtca
     6421 ccgccgccga tgtcgagcac gcggtgcggc tgtccgtcac cgcgctgcgc gccgggctcg
     6481 ccgcggactg gagcgcgccg gccggcacgc tggagtggga ctgctgggag accgtcgaac
     6541 acctctcgga cgacctcttc gcctacgccg tgcagatcgg cccggcccag cccccgctgg
     6601 acggcgaggt cccctatctc tgggaggcca ggcgtcccgg cggtccgcgc aacgcggtac
     6661 acgcggaccg tgccgcgggc ccggccgggc tcctgcagac cctggaggcg agcggcgccc
     6721 tgctgaccgc gatggtgcgc accgcctcgc ccgaggtacg ggcgtaccac ggtttcggcg
     6781 tctcggaccc ggaggggttc ggtgcgatgg gcatcgtcga gacgctgctg cacacccacg
     6841 acgtggccga gggactcggc ctggcctgga ctccgccgac cggtctgtgc gaccgggtgc
     6901 tggcccggct gttccgggac gtgctggagg acccggaccg ctggcgggtg ctgctgtggg
     6961 ccaccggccg cgcggaccta gcggggcgga cccgcccgga caagtggcgc tggtacggcg
     7021 agccgctcag ttcgggcgca gccacaccct gagcggcccg gccggggcga agcccgcgcg
     7081 cagtgcggcg gccaggtcgt cgccgctctc gtagccgacg acctcgcggc ccggccacag
     7141 tcgggcgacg gtggcgaggc agcccgccca ggccgcgtcc tggtcgccgt cgacggcgta
     7201 caggttggag atgcccacga cgtccccgct ccggctggcg acggctcccg cggtcgcctc
     7261 tccccgctcg tcgcgctcca ccaggaagga gtggccgggt tcggcgagca gcgcgggcgg
     7321 gaagagccct tcgctcccgc cgtcggacca cgcccgctcc caggcggcca actcgccctc
     7381 gccgtgcacg agttgccagc ccttgccggt gataccggga gccgcccgat ggatccactg
     7441 cgcctcgaag aggacctcga agccgtcgtc gctcaggtcg aggacggcaa agctgtcctt
     7501 cacggacgcg ccgggtgcgg tcaggtcgat ggagccgagg acgtcaccgg gcgaggcctc
     7561 gggggtgagc gtcaccgcgt ccggatagta gagcggggtg cggcgcgggc tggtccaggc
     7621 gtacggcccc tcgcgccccg tgatgccgtg tgcgcggcag agcgcggcgc accagtcggc
     7681 gttgttgcgg gcggcttcgc gcgcctgccc ggtcgtgtcg ctctccatgg tcacggcccg
     7741 atcctgcctt gccgggccgg gccgtgacca gcggatttca ggcgcccagc ggggcggccg
     7801 cgtcgagcgc cgggtagtcg gtgtagccgc ggtcgtcgcc gccgaagaag gtcgacgggt
     7861 cgggggcgtt gaacggaccg cccgcccgca gccgggccgg caggtccggg ttggcgagga
     7921 agagcgcgcc gtaggagatg aggtcggcgg tgccgtcctc gatcagggtg agcgcgtccg
     7981 gcccggtggg cccttcggtg gccgcgttga ggatgaacac gccggagaac gacttgcgca
     8041 gccggagcgt caactcccgg ctctccggag cgacttcgag aacgtggagg taggcgaggc
     8101 cgagcggctc aagggcggcc acgagcgcgg tgtagaccgg gtcgggctcg ggctccacga
     8161 tgccgttgta cgggttgccg ggcgagaggc ggatggcggt gcgctcggcg ccgatctcgg
     8221 ccgccacggc cttggtcacc tcgacggcga accggatgcg gccctcgacc gaaccgcccc
     8281 aaccatcggt gcgcacattg gagttgggcg cgaggaactg gtggatgagg taaccgttgg
     8341 cgccgtgcag ctcgacgccg tcgaagccgg cctcgatcgc attgcgcgcg gcggccgcga
     8401 agtcgccgat ggtgaccagg acctggtcct cgctgagcgc ctgcggggtg atgaagggct
     8461 tggggccctc gtgcgtgaag acctgcccgt cggcggccac ggcggacgga cccacgttga
     8521 tcaggtcgcc gggcagcaga tcggggtggc cgatccggcc cgcgtgcatg agctgggcga
     8581 agatccggcc gcccttggcg tgcacggcat cggtgacctt gcgccaggcc gcgatctgct
     8641 cgtcgctgtg cagccccggg gtgtcggggt agccctggcc gaccggggag ggctggacac
     8701 cctcggtgat gaggagcccg gccgaggcgc gctgggcgta gtactcggcg gtcgacgcgg
     8761 tcggcgagtt gcccggcccg gtggcgcggc tgcgggtcat gggcgccagg gcgatgcggt
     8821 tggcgagcgg ggtgccggcc aggtcgatcg gatcgaaagc ggtggtcaca gcggcctcca
     8881 gggagacaag ggagagtatg cggccgaccc cgaaggttta tgtggtcggc caagtatcgg
     8941 tgccgaaagc cactgtagcg cattacttgg ccgaccaagg taatcttgtc gcatgacctc
     9001 cgacgagccc gtctgtaccg atttccccca cctgccgagc gcggcccgcg gcggcccgtt
     9061 cagccatgcg gtctcacggg tggcccggct gcaccgcatc gccgcgggcc gtcagctgcg
     9121 ctccatcggg ctctacccgg ggcaggagct cctgatgatg cacctgtggg acgcgggggc
     9181 ggcccgtcag tccgagctga tcaagctgct tgagctcgac ccgtcgacgg tcaccaagat
     9241 gctccagcgc ctggagcagg ccggctatgt ccggcgcagc cccgacccga aggaccggcg
     9301 cgccgcgctg gtcgaggcga ccgacgacgg ctgcgtcctg cacgagcgcg tgcagcaggc
     9361 ctggacggac ctggaggagc agaccctggc gggcctggac gcggccgaac gggccgagct
     9421 ggcaaggctg ttggggaagg tggaggccaa cctgtgccgc tcggcgggcg ccgcggccga
     9481 ctgcgaggcc gaaccgtcct gcccgagcga ggcgggctgc ggggagtgag gcgacccgcg
     9541 gggagccggg cgactcccgg ggggtgagcc cggccggggt gtcaactccc ccggccggac
     9601 gggaacttcc gcggacgggc gcttcagcgg gcgggcacgt cagcgagcgg gcagcagctc
     9661 caggaggcgg tccacgtccg cggccgagct gtacagatgg aaggccgcgc gcagattgcc
     9721 cgcgcggttg gagagttcga cccccgcacg gctcagctcc tcctggtggt gaccgaggcc
     9781 cgggaccgcc acgatcgccg agccgggcgc ggccaccggc tcgtggccga gcggggcgag
     9841 gccggcgcgg aagcggtcgg cgagcgccgt gttgtgcgcg tggatgcgct cgatgcccac
     9901 ctcctcgacg agggccagcg aatgccgggc cccggtgtac gagaacagcg ccgggctctc
     9961 gtcgaaccgc cgcgcggagt gcgccagttc gtcgatgggg ccgtagcagg cgtcccaggg
    10021 gtgctcgccc gccacccagc ccgcgaagat cgggttcagg tcgccgaggt cgtcggggac
    10081 ggtcaggaag gcgaccccgc gcggggcggc cagccatttg aagccgaccg tcacggtgta
    10141 gtcgtacgcc ccggcgtcga ccgggtacca gcccgcgccc tgggacacgt cgaccagggt
    10201 ccgggcgccg tgggcgcggg cggcctcgcg gatcgcggcg aggtcggcga tgcggccgtc
    10261 cgcggactgg accgcgctga ccgcgaccag ggcggtgccc ggccgcaccg actcggccag
    10321 ccgctccagg ggcgcggtgc gcaccttcag gtcccggcgc atgtggaacg ggttcaccac
    10381 ggagctgaag tcggcctcgg cgacg
//
