LOCUS       NZ_CP070373            10228 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5585108
            Orig. end                         :: 5595336
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="31"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5228
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    611..624
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            1493..1969
                     /locus_tag="GWQ54_RS24610"
     CDS             1493..1969
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069408.1"
                     /locus_tag="GWQ54_RS24610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_161245321.1"
                     /transl_table=11
                     /translation="MTVDTLDTRILRLLIEQPRTSIREYARLLGIARGTLQARLDRMER
                     DGVITGTGPMLSPAALGHPVLAFVHIEVTQGHLDEVGDALAAVPEIIEAFSITGGGDLL
                     TRVAARDNAHLEDVIQRLIQLPGVVRTRTEVALRERVAHRLLPLVESVGRAAAK"
     gene            2010..2702
                     /locus_tag="GWQ54_RS24615"
     CDS             2010..2702
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320471.1"
                     /locus_tag="GWQ54_RS24615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="WP_167155486.1"
                     /sec_met_domain="HAD_2 (E-value: 1.6e-36, bitscore: 124.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSHNTSVIFDLDGTLVDSEPNYYEAARQVLARYGLPDFSWEQH
                     ARFIGIGTRETLEILDRQHTLTGPIEEVLAAKNAAYLELARTSTEVFPEMRAFVERLHA
                     DGVRMAVASGSSRSAIDAVLAGTGLASFMDLVVSAEEVEHGKPEPDVFLEAARRLGAAP
                     ADCVVLEDAPPGAAAAHAAGMRCIAVPYLPQSSGDPAFATAGLLFPGGQREFSAERAYA
                     WMDATSTA"
     misc_feature    2796..2811
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.25"
                     /tool="antismash"
     gene            2914..3966
                     /locus_tag="GWQ54_RS24620"
     CDS             2914..3966
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660526.1"
                     /locus_tag="GWQ54_RS24620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chitinase"
                     /protein_id="WP_167155481.1"
                     /transl_table=11
                     /translation="MIGRTLRLLGAGLVLALATQVPVAAAPSAPQADTCAVKSRPSGKV
                     LQGYWENWDGASNGVHPPLGWVPITDARIAAHGYNVINAAFPVIRSDGTVLWQDGMDAT
                     VKVPTPAEMCQAKASGQTILMSIGGAAAGIDLSSSAVADRFVASIVPILKQYNFDGIDI
                     DIETGLVGSGNINTLSASQANLIRIIDGVLAQMPAGFGLTMAPETAYVTGGSVTYGSIW
                     GAYLPIVKKYADNGRLWWLNMQYYNGSMYGCSGDSYEAGTVAGFTAQTNCLNKGLVVQG
                     TTIKVPYDKQVPGLPAQPGAGGGYMTTSQVAQAWNTYGGGLKGLMTWSLNWDGSKNWTF
                     GNNVKALQGR"
     misc_feature    2929..2931
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4054..4530)
                     /locus_tag="GWQ54_RS38545"
     CDS             complement(4054..4530)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /locus_tag="GWQ54_RS38545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="luciferase family protein"
                     /protein_id="WP_167155478.1"
                     /transl_table=11
                     /translation="MTAAHRAMDQLESWPNLVTGPPRCAVGRALFAAECEVVHFHSGFA
                     ADLHLTHPAIERLRPELRHSTAIRLKPGSAWVTVLLDCGADVDLLLTLVSMALKAQALS
                     PPNALAAPCTWYRPAVPPQRVADPPVLTLGPRPGVLPAARRAVGRFLPHGPRAV"
     misc_feature    4920..4929
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.67"
                     /tool="antismash"
     misc_feature    complement(4925..4938)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            5001..5228
                     /locus_tag="GWQ54_RS24630"
     CDS             5001..5228
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157700.1"
                     /locus_tag="GWQ54_RS24630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type A2 lantipeptide"
                     /protein_id="WP_167155475.1"
                     /sec_met_domain="strepbact (E-value: 1.9e-28, bitscore:
                     97.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAPQVQTLEISDADLDNVSGGLVGTALGNVTGTADSVLPVSGI
                     VNSVTGLVGSTTGVNTGAVTGLATGLVAGL"
     misc_feature    5269..5287
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.93"
                     /tool="antismash"
     gene            5332..6138
                     /locus_tag="GWQ54_RS24635"
     CDS             5332..6138
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="GWQ54_RS24635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_167155472.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGRLVLFVTVVVMAAASFW
                     AVTGSVSSTIDAPGILTHAQGSYVLQSPVAGQVTGVLAKEGESLAADAPLLKVRTAQGE
                     RIVRAIAAGRLTSLAATIGAVVATGSNVASVERTGGADDPLTVMLYVPGDRAASVPVGA
                     PVDLTVQSVPADRYGVLRGTVAAVGRTAQTPQQIAAFLGDGQLAEQFARDGRPVGVAVR
                     LDRAPGTKSGYRWSSADGPPYAVDSMTPATGTVHLADRRPIDWLLP"
     misc_feature    complement(5355..5367)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     gene            6267..8411
                     /locus_tag="GWQ54_RS24640"
     CDS             6267..8411
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558043.1"
                     /locus_tag="GWQ54_RS24640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_275901409.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.1e-35, bitscore:
                     121.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRSPTVLQMEALECGAASLAMVLAHHGRHVPLEELRIACGVSRD
                     GSRASNVLKAARGYGLTAKGMQMEPAALAEVQAPAILFWEFNHYVVYDGTGRRLGRRGV
                     HINDPDKGRRFVASEDFDTSFTGVVLVLEPGPDFRPGGRKPGVLGALPARLRGTTGTML
                     AALLASLLLVAVGATLPALSRTYIDLFLIGHQTSLLGVLFAAMGLMVVLTVVLTWLQQA
                     NLLRGRIISSTLSSARFFRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLTAAA
                     VDGVVVLLYAVLLWTYDPQLTVVGVGIALLNVVAMRIVVRLRATDTQKLRADSARLTNT
                     SYTGLQLIETMKATGGENGYFRRWAGQHATTLEVQQRLGVPSAWLGVVAPALATLNSAL
                     ILWIGGLRAVEGHLSIGLLVAFQALVTRFTAPVTRLNGVAGRIQDFAADVARLKDVENF
                     PVDALHSRPEPPASTRRLKGHVTLDDITFGYSPLDKPLLTGFSLTVGPGQQVALVGGSG
                     SGKSTVSRLISGLYSPWEGTIRIDGRRLEDIPRGALAASVSFVDQDVFLFEGTVRDNVA
                     LWDPSIPDDAVVLALRDAELYDVVARRPGGIHSRVEQDGRNFSGGQRQRLEIARALVRR
                     PSILVLDEVTSALDAETEHLIIDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGTVVER
                     GRHEDLVGAAGPYARLVREH"
     misc_feature    8761..8770
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(9752..9767)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
ORIGIN
        1 cgggtcggcg gccaccgcgg ccaggccccg cacctcgcgg gcgcactcgt cgagcaggac
       61 caggacctgg tgggcacggg cccggcgggc ccgaagcggg ctgagggggt ggaccagcgg
      121 ggcgagcgag aggcgcaccc ggatgagcag cagctccagt tcggcggcgc ggggcgccgg
      181 gtcggcgctc tcgtccccgg cgagccggtg ggcggccgcc gcggtgcacc gacggacgca
      241 gtgcagggcc cgctggatcc acgcgtcggt ggcggtgtgc gtcgtgaccg gcagcacgac
      301 ggcgacgccg atgaccgcgc cgagcgcacc caccgccgtc tcctggacgc ggagcacgag
      361 gagcccgggg tggagcacac cgaggagccc gtagagcagg ccggccatga cggtgacgaa
      421 gaacatcatc cagctgtagc tcggcgcggc cgtgtagaag atcccgaaga cgcacaccgc
      481 gaccagcgcc gcggtcgggg ccggcgcccc gtgcagcggg atcgcgatga gcagtccggc
      541 cacgatgccg acgaccgtgc cgaggacccg gcggaacccg cggaccaggg tctcgccgcg
      601 cgaggcggtg ttgacgaaga tccaccaggc ggcgccgacg gcccagtacc agcggtcctc
      661 ggagagcacc tggcccacac cgagcgcgaa gccgcaggcc acggtcgcct ggaaggcctg
      721 ccgggtggtg tggcgggcaa gtccccggcc ggccagcggg gccggggccg cgggctgcgt
      781 gatgtggcgc tcgatgcacc acagaccgaa cctgaccgac gccgaggcgg cgatcgccag
      841 cgcgatggcg ccgtacagct cgggcagttg ggaggtgacc gcgtgcagga actgggtcgc
      901 gaagaacatc atgaaggcga agatgccgag cgcatgtccg cgcgggcccc agcgcctggc
      961 gtacaccccg gcgaagatca ccacgagcca ggcggcgtcg cgcgccatcg gggtgccgtg
     1021 cagcgcggcc gcgagggcga gcaccgggaa gccgacggcg ggcagcagcg cggtggtggt
     1081 gatctgggcg cggacggtgg cgtcgccaac ggtgaacagc gcgagcagcg cggcgaggcc
     1141 ggcggtgatc gacgcggcga gcgagagccc gcacagttcg gcggcggtca ccgagaggcc
     1201 cacgccgagc acggctcgca gggacacccg cagtcgcaga agccccgggt ccggagccac
     1261 aaacatcctc ttcacggcgg tcgccgcgcc cccttacgtg caatcggtct caggcatgac
     1321 aaaggcgccg cggggcccgg accggcttcg tcgccgtccc gcgcgctgcg cggcgccatc
     1381 taccaccaga ggaaaacacc cggagggaca gtggctcaac cgagctccgt gtcgctgggc
     1441 cattggtaca gtcgatggcc cagtcgcagg accaggaagg agccaacggg acatgacggt
     1501 ggacacactc gacacccgca tcctgcggtt gctcatcgag cagccgcgca ccagcatccg
     1561 cgagtacgcg cgcctcctgg ggatcgcccg cggcacgctc caggcgcggc tcgaccggat
     1621 ggagcgggac ggggtgatca ccggcaccgg ccccatgctc tcgcccgcgg ccctcggcca
     1681 cccggtgctg gccttcgtcc acatcgaggt gacccagggg catctggacg aggtgggtga
     1741 cgccctcgcg gcggttcccg agatcatcga ggcgttctcg atcaccggcg gcggcgatct
     1801 gctgacccgg gtcgcggccc gcgacaacgc ccatctcgaa gacgtcatcc aacgcctgat
     1861 ccagctcccg ggcgtcgtac gcacccggac cgaggtggcg ctgcgcgagc gggtggcgca
     1921 ccggctgctg ccgctcgtgg agtcggtggg ccgggccgcc gcgaagtgag cggcgcggaa
     1981 cgccgccgac ccttggcatg ctgggacaca tgagcacatc gcataacact tcggtcatct
     2041 tcgatctcga cggcaccctc gtggacagcg agccgaacta ttacgaagcc gcgcgtcagg
     2101 tgctcgcgcg ctacggcctg cccgacttca gctgggaaca gcacgcccgc ttcatcggca
     2161 tcggcacccg cgagaccctg gagatcctgg accggcagca caccctgacc ggcccgatcg
     2221 aggaggtgct ggcggccaag aacgccgcct atctggagct ggcccggacc tcgaccgagg
     2281 tcttccccga aatgcgggcc ttcgtggagc ggctgcacgc cgacggcgtc cgcatggcag
     2341 tggcctccgg ctcctcgcgg tccgcgatcg acgcggtcct ggcggggacc gggctcgcct
     2401 ccttcatgga cctcgtggtg tcggcggagg aggtcgagca cggcaagccc gagcccgacg
     2461 tcttcctgga ggccgcccgg cgtctgggcg ccgcacccgc cgactgcgtg gtcctggagg
     2521 acgccccgcc cggagcggcg gccgcgcacg ccgccgggat gcgctgcatc gccgtgccgt
     2581 acctgccgca gagttccggc gacccggcgt tcgccaccgc cggcctcctc ttcccgggag
     2641 gccagcggga gttcagcgcc gagcgggcgt acgcgtggat ggacgcaacc tcgacggcct
     2701 gagcccatga gccggatccg gctcaactcc cgctgtgcgc aagggaactg atgagctcgc
     2761 aggtccatgg cctagcgcct ccggcacgat tgacggaagg tctggaccaa tctacggtgt
     2821 ggggcgccac cccctcgtac ctcctcaggt ccccccacgg tccaggagcc gcgcggcctc
     2881 ttcgcgcccg ctcccggagg caaggagcac agcatgatcg gtcgtacgtt acgtctgctg
     2941 ggagccgggc tcgtcctggc gctcgccacc caggtcccgg tggccgcggc gccgagcgca
     3001 ccccaggcgg acacctgcgc ggtgaagtcg agaccctcgg gcaaggtgct ccaggggtac
     3061 tgggagaact gggacggcgc gtccaacggc gtccatccgc cactgggctg ggttccgatc
     3121 accgacgcgc gcatcgccgc ccacggctac aacgtcatca acgcggcgtt cccggtgatc
     3181 cgttcggacg gcacggtgct gtggcaggac ggcatggacg cgacggtgaa ggtccccacc
     3241 cccgccgaga tgtgccaggc gaaggcgtcc ggccagacca tcctgatgtc gatcggcggg
     3301 gccgccgcgg gcatcgacct cagttcgagc gcggtggccg accggttcgt ggcgtcgatc
     3361 gtgccgatcc tcaagcagta caacttcgac ggcatcgaca tcgacatcga gaccggcctg
     3421 gtgggcagcg ggaacatcaa cacgctgtcg gcctcgcagg ccaacctgat ccgcatcatc
     3481 gacggcgtgc tcgcccagat gccggcgggc ttcggcctca cgatggcccc ggagacggcg
     3541 tacgtcaccg gaggcagcgt gacgtacggg tcgatctggg gcgcgtacct gccgatcgtg
     3601 aagaagtacg cggacaacgg ccgcctgtgg tggctgaaca tgcagtacta caacggcagc
     3661 atgtacggct gctccggcga ctcgtacgag gccggcacgg tcgccgggtt cacggcccag
     3721 accaactgcc tcaacaaggg cctggtcgtg cagggcacca ccatcaaggt cccctacgac
     3781 aagcaggtgc ccggactgcc cgctcaaccg ggcgccggcg gcggctacat gacgacaagt
     3841 caggtggcgc aggcgtggaa cacctacggg ggcggtctca agggtctgat gacgtggtcc
     3901 ctcaactggg acgggtcgaa gaactggacg ttcggcaaca acgtcaaggc gctgcaaggg
     3961 cgttgaccgt ccgagacggc caccgtccgg gccccgtccg cgctccagcg ggcggggctt
     4021 ccgcctgtcg gcggccccct gcgcacgctc cgttcatacc gcgcgcggcc cgtgcggcag
     4081 gaaacgcccc accgcccgcc gcgccgcggg caggacaccc ggcctcgggc cgagcgtgag
     4141 caccggcgga tcggcgacgc gttgcggggg gacggccggg cggtaccaag tacagggcgc
     4201 ggccagcgcg ttcggcggac tgagcgcctg cgccttcagc gccatgctga ccagcgtgag
     4261 cagcagatcc acgtccgcgc cgcagtcgag caggaccgtc acccaggccg agccgggctt
     4321 caggcggatg gcggtgctgt gccgcagctc ggggcgcagc cgctctatcg cggggtgggt
     4381 gagatgcagg tccgccgcga agccggagtg gaagtgcacc acttcgcact ccgcggcgaa
     4441 gagcgctcgt ccgacagcgc atcggggtgg tccggttacc aggttcggcc atgactccaa
     4501 ctgatccatg gcgcgatgag ccgcggtcat ggcgccatcc tcgcgcacac ttcaccggca
     4561 ggaccacccc cgtccacccc cgggatgcgt ctcgtggccg atccgtgccc gcgggttggc
     4621 cgggaccgtg gccgacgtgc cccacagaca agcggaggga caccttccga gaatctgcac
     4681 agggtgtgcg cggacccgtc accggggccg cgagcccgct cccgcgccgc cgaaagcgcg
     4741 cggcattggt gcgggagtgt cccagttgcg agaagcgatt ccggggcccg gggaattcgt
     4801 cccgtgaagc tcgtgaagga aatgcccccg aatcatgaac gggtctgcac gggacggcaa
     4861 ttcaatgccg gaagaaagcc ctgtccatcc ccccgccgat tggctgccgc cgccttcggc
     4921 gtgttttgat cctggccact gcgggagccc cggcaaggag ttcccgatag gaaatgtccc
     4981 catgaaaggc agtactcacc atgaacaacg ctccccaggt ccagaccctc gagatctccg
     5041 acgccgacct ggacaacgtg tccggcggcc tcgtgggcac cgctctcggt aacgtgaccg
     5101 gcaccgccga ctccgttctt ccggtctccg gcatcgtcaa ctcggtcacc ggcctggtcg
     5161 gttcgaccac cggtgtgaac acgggtgccg tcacgggcct ggccaccggt ctcgtcgccg
     5221 gtctctgaga tcgcgcacga gccccggaac cgctcggttc cggggctcct tgcgaatggt
     5281 tcacgctttg cggaagtttc gcgggcaagg aacgggtaag gaaagagttc cgtgcagttt
     5341 cgtcagcagg ccctttccaa actgcagtca ccggaggaac tcgacctgcc ggtgcgtttc
     5401 gcacgcccgc agggacgact cgttctcttt gtcaccgtgg tcgtcatggc cgccgcctct
     5461 ttctgggccg tgacgggctc cgtttcctcc acgatcgacg cacccggcat cctcacgcac
     5521 gcccagggca gttacgtcct gcagagcccg gtcgcgggcc aggtcaccgg cgtgctcgcc
     5581 aaggagggcg agagcctggc cgccgacgcc ccgctcctca aggtccgcac cgcgcagggc
     5641 gagcggatcg tccgcgcgat cgccgcgggc cgcctcacca gcctcgccgc caccatcggc
     5701 gccgtcgtcg ccaccggctc gaacgtggcg agcgtcgagc ggacgggcgg cgccgacgac
     5761 ccgttgacgg tcatgctgta cgtgccgggc gaccgtgccg cgagcgtccc cgtcggcgct
     5821 ccggtcgacc tcaccgtgca gtccgtgccg gccgaccgct acggggtgct gcgcggcacg
     5881 gtggccgcgg tcggcagaac ggctcagacc ccgcagcaga tcgccgcgtt cctcggtgac
     5941 ggacagctcg ccgagcagtt cgcgcgggac ggcaggccgg tgggggtcgc ggtccgcctg
     6001 gaccgggcgc ccggcacgaa gtccggctac cgctggtcgt cggccgacgg cccgccctac
     6061 gcggtcgact ccatgacccc ggccaccggc accgtccacc ttgccgaccg gcgccccatc
     6121 gattggctgc tcccgtgacc gcaccccacg cctcaccccc caccccccgg ctgccggccg
     6181 ggcacggccg gcggcgcccg gccaacaccc cttcgggaga ccgccgttcg cgcgcgcggc
     6241 aggccccgaa ggcccggccc gccaaggtgg tgcgcagccc cacggtcctc cagatggagg
     6301 ccctggagtg cggcgccgcc tccctcgcca tggtgctcgc ccaccacggc cgccatgtgc
     6361 ccctggagga gctgcgcatc gcctgcggcg tctcgcgcga cggctcgcgg gcgagcaacg
     6421 tcctgaaggc ggcgcgcggt tacgggctca ccgccaaggg catgcagatg gagccggccg
     6481 ccctcgccga agtccaggca cccgccatcc tgttctggga gttcaaccac tacgtcgtgt
     6541 acgacggcac gggccgtcgg ctggggcggc ggggcgtgca catcaacgac ccggacaagg
     6601 gccgccgttt cgtcgcgtcg gaggacttcg acacgagctt caccggcgtc gtcctggtcc
     6661 tggaacccgg cccggacttc cggcccggcg gcaggaagcc cggtgtcctc ggtgcgctgc
     6721 ccgcccggct gcgcggcacc acgggcacga tgctggccgc actgctcgcc agcctgctcc
     6781 tggtggccgt cggcgcgacg ctccccgcgc tgagccgcac gtacatcgat ctgttcctga
     6841 taggtcatca gacgtctttg ctgggagtcc tgttcgccgc gatgggcctg atggtggtgc
     6901 tcaccgtggt gctgacctgg ctccagcagg ccaacctgct gcgcgggcgg atcatctcct
     6961 ccacgctcag cagcgcccgc ttcttccggc atctgctgcg gctgccggtc acgttcttcg
     7021 cacagcgcag ccccgccgac ctcgtgcagc ggctgcagtc caacgacgcg gtggccgaga
     7081 cactggcccg cgatctgacg gcggcggccg tggacggcgt cgtcgtgctg ctctacgccg
     7141 tcctgctgtg gacctacgat ccccagctca ccgtcgtcgg ggtgggcatc gcgctgctca
     7201 acgtcgtcgc gatgcgcatc gtcgtacggc tgcgggccac cgatacccag aagctgcggg
     7261 ccgacagcgc ccggctcacc aacacctcgt acaccgggct ccagttgatc gagaccatga
     7321 aggccaccgg cggcgagaac gggtacttcc ggcgctgggc gggccagcac gcgaccaccc
     7381 tggaggtgca gcagcggctc ggggtgccga gtgcctggct cggggtggtg gcgcccgcgc
     7441 tcgccaccct caacagtgcg ctgattctgt ggatcggcgg gctgcgggca gtggaggggc
     7501 acctgtcgat cggtctgctc gtcgcgttcc aggccctggt gacgcgcttc accgcgccgg
     7561 tgacccggct gaacggggtg gcaggccgga tccaggactt cgcggccgat gtcgcccggc
     7621 tcaaggacgt cgagaacttc cccgtggacg cgctgcacag ccgcccggag ccgcccgcga
     7681 gcacccgccg cctcaagggc catgtgacgc tcgacgacat cacgttcggc tacagcccgc
     7741 tcgacaagcc cctgctcacc ggcttctcgc tgaccgtcgg gccgggtcag caggtggctc
     7801 tggtcggtgg ctcgggaagc ggcaagtcga cggtttccag actgatttcg ggcctgtact
     7861 ccccctggga gggcaccatc cgcatcgacg gccggcgcct ggaggacatc ccgcgcgggg
     7921 cgctcgccgc ctccgtctcc ttcgtcgacc aggacgtctt cctcttcgag ggcacggtgc
     7981 gggacaacgt ggcactgtgg gacccctcga tcccggacga cgccgtggtc ctggcgttgc
     8041 gggacgcgga gctgtacgac gtcgtcgcgc ggcggcccgg cggcatccac agccgggtcg
     8101 agcaggacgg gcgcaacttc tccggcggcc agcgccagcg cctggagatc gcgcgggcgc
     8161 tggtgcgccg tcccagcatc ctggtcctcg acgaggtcac cagtgccctg gacgcggaga
     8221 ccgagcacct catcatcgac aacctgcgcc gccggggctg cgcctgcgtc gtcatcgcgc
     8281 accggctgag cacggtccgc gacagcgacg agatcgtggt cctcgaccac ggcacggtcg
     8341 tggagcgggg ccgccacgag gatctcgtcg gcgcggccgg gccgtacgcc cggctcgtga
     8401 gggagcactg agatgagctc cgtacatccc ctcgcccagc cgggcaccgc cggtcaggac
     8461 gccgtggtcg gcgcgctcgg cggcctgggg cagggcgtcg actgcgccgg gctgcgcggc
     8521 ttctccctcg aagggccgca ggtgctgtgg ctggtggtga gcggcgccct cgacctgttc
     8581 gcggtggacg cggcggagca gggccactgg cacttcctcg gccggctcga accgggcacc
     8641 ctgctgctcg gccccgtcga gggcccgcag cacaccctgg tcgggcgccc gctgcgggag
     8701 tgcgtgctgc gccgcatccc gctgcgcgag ctgtaccggc atccgcaatc cggcggcccc
     8761 gaaggcggcc acgacggcgg actcagccgg tacgacaccg gggacgcggc cctcagtctc
     8821 ctggagcacg ccttctcgct cggcgtcggg cggggccaac gggtgttgtt cgaagccccg
     8881 ttggacggtc gcaccccgct ggacgagtcc gtggcggacg acgacatcct gtggcttccg
     8941 gtggcaccgg gcagcgtgca gtacggggcg gccttcagcg cggaggcggc cggggacctg
     9001 ctcgtcgacg gtggcttgtg gcagggcatg gtcaaccagc agtaccggct gctcgccgcg
     9061 ctcgaccgct ggatcgagcg ggtggagcgt gcccacgagg accgcgcggc cgccgggatc
     9121 aaggccggtg aggcggtccg cgaggaggcc gaccgggcgc tgctcagctc catcggccgc
     9181 cccggcaggg cgagttcacg cgcggcggcg tccgacgact cgacgtacgc cgcctgccgc
     9241 ctggtcgcgg aggcggccgg gatcaccctc ggcgacccgg ccctcggcgg cgcggtcagc
     9301 gaccggatcg acccggtcga gcgggtcgcc gtcgcctcgc gcgtccgcac ccggccggtg
     9361 cggctcgacg gccgctggtg gcgcgaggac gccgggccgc tcgtcggcca ccgcacgagc
     9421 ggcaccccgg tcgccctgct gtggcggcgc ggcggctacg aggcggtgca tccggtgtcc
     9481 gggcggcgca cccgggtgga cgcgggcaac gccgaggagt tcgagcggcg ggccgtgatg
     9541 ttctaccggc cgctgccgga ccgcccgctg agccggtggg ggctgctccg gttcggcctg
     9601 cgcggcaccc gccccgacgt gcgcaggctg ctgctcgccg gtctggtgac ggtggggctc
     9661 ggcgcgctcg tgccgatcgc gaccggtcag gtgctcgggg tgtacgtgcc gcgggcgcag
     9721 aacggcctca tcgtgcaggt ctcgctcgcc gtgatcgtca ccggcgtggt gtcggcggcg
     9781 ttcacgctgc tccagaacct caccgtgctg cgcatggagg gccggatcga aagcaccctt
     9841 caacccgccg tctgggaccg gctgttgagg ctgccgacca cgttcttcgc cgggcgttcc
     9901 accggcgagc tggcgagcgc cgcgatgggc gtgagcgcga tccggcgggt cctttccggg
     9961 atcggcccgg tcgcggtgca ggcgggcacg gtcggcgcga tgaacctggc tctgctgctg
    10021 tgcttcagcg tgccgctggc gttggtcgcg atcgcccttc tggtcgtcat cgccgccgtg
    10081 ttcctcgcgc tcgggatgtg ggagctgcgc tggcagcgcc gcctcgtgga gctgggcaac
    10141 aggctcaaca accaggcgtt ccagaccctg cgcgggctgc ccaagctgcg ggtcgccgcg
    10201 gcggagagct tcgcgtacgg ggcgtggg
//
