LOCUS       NZ_CP070373            10390 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5306654
            Orig. end                         :: 5317044
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="28"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    423..436
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    818..833
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.03"
                     /tool="antismash"
     gene            1001..2161
                     /locus_tag="GWQ54_RS23410"
     CDS             1001..2161
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005752.1"
                     /locus_tag="GWQ54_RS23410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase"
                     /protein_id="WP_167160085.1"
                     /transl_table=11
                     /translation="MVIRSGFRSMAALTATAAIGLCWLAPTPDSSASSPAQVPGAGQQV
                     SPRPGAPALPGDVSALSYVVADARTGKVLAARDAHRKLPPASTLKTLFAVTALPHLHAE
                     DRHTVSEHELAGIGPGSSLVGVEAGHTYQVSDLWRGVFLSSGNDAVHVLASLNGGWEET
                     AAQMQAQARRLGALDTEVVSPDGYDTPGQVSSAFDLAVFGRAGLARPDFAEYCSTASAQ
                     FPGGGGSYGIMNTNRLLSGTDGISRYPGIVGVKNGYTTNAGNTLIAAAKQGDRTLLVTV
                     MNPQAGGGFAVYDEARTLLDWGFAAWDHVDSVGSLEPVRAKSPQRQAQIAHVAAVKPEA
                     AADDDEPGWTLTSSAAAGIAVAGTLFLVVRRRRAVHARTERSRPTE"
     misc_feature    2384..2398
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.59"
                     /tool="antismash"
     misc_feature    2416..2427
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.75"
                     /tool="antismash"
     misc_feature    complement(2452..2466)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            2624..3070
                     /locus_tag="GWQ54_RS23415"
     CDS             2624..3070
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430726.1"
                     /locus_tag="GWQ54_RS23415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="subtilase-type protease inhibitor"
                     /protein_id="WP_167160083.1"
                     /transl_table=11
                     /translation="MRKIAAAGAVTVALLANGLIGTGLGSAAAAPAEAMKPLSLYPPSA
                     LVISIEPGTGDQGASVQRAVTLSCAPTPSGTHPAPGAACAELKGVQGDFNALVASGAPR
                     LCTQVWQPVTVRADGVWNGKRVSHVQTYANSCLKAAGSEAVFNF"
     misc_feature    complement(3243..3256)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            3275..4366
                     /locus_tag="GWQ54_RS23420"
     CDS             3275..4366
                     /EC_number="3.1.4.-"
                     /GO_function="GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712508.1"
                     /locus_tag="GWQ54_RS23420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="WP_205380418.1"
                     /transl_table=11
                     /translation="MSDPRPRRFTPRPRTALLGALAACLALATAGAGGSASAAGPETLP
                     LSGATVSGLHNTYDPAAFSYLAQALDSGTSMIELDVWDDFITKEWKVSHSNPLGNSNNC
                     VNASTPAQLYTGGANKNLESCLDDVRVWLGAHPGHPPLVIKLEMKAGFQATYGMSPAKL
                     DQTISDHLGSAVFRPADLLAKPGGGQYATLDEAAGAGNWPTRQALAGKVMLEVIPGTVE
                     EGNPTDTLWTDVEYARYLRDLAAAGRTGQAQIFPAVHNAQAGDPRSRYADASIRPWFVV
                     FDGDAATYLGGTIDTAWYDTHHYFLVMTDAQNVAPALDDKNPSPADAQARVARLAAAHA
                     SVASNDWTSLPQIQSLALPRGVG"
     misc_feature    4669..4678
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.11"
                     /tool="antismash"
     misc_feature    complement(4720..4737)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.58"
                     /tool="antismash"
     misc_feature    4727..4741
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.6"
                     /tool="antismash"
     gene            5001..5390
                     /locus_tag="GWQ54_RS23425"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762077.1"
                     /locus_tag="GWQ54_RS23425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_161253115.1"
                     /sec_met_domain="melC (E-value: 6.6e-45, bitscore: 151.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQPSRPSRPTRRQAIGVTAGTLAGLALAGTAHAAVPSGRTDAHT
                     GHDAPEAFDEVFQGRRIQGAPAAGHMHHGGGYGVRIDGQELHVMQNADGTWISVVNHYE
                     TFATPRAVARAAVVELQGASLVPLA"
     gene            5445..6272
                     /locus_tag="GWQ54_RS23430"
     CDS             5445..6272
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038020.1"
                     /locus_tag="GWQ54_RS23430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_161253112.1"
                     /transl_table=11
                     /translation="MAVRKNQADLTATEKRRLVDALLQLKRSGRYDTFVSTHNAFIMSD
                     TDTGDRVGHRSPSFLPWHRRFLIEFEQALQSVDASVTLPYWDWTVDRTPTASLWAADFL
                     GGTGRSRDGQVMDGPFAQSAGGWPLNVRVDGRTYLRRALGASVAQLPTRAEVDSVLAMT
                     TYDMAPWNSASDGFRNHLEGWRGVNLHNRVHVWVGGQMATGASPNDPVFWMHHAFVDKL
                     WSQWQTKHPASPYLPAAGTRDVVDLHDTMRPWNNVTPADMLDHRGYYTYDTAA"
     gene            complement(6295..7095)
                     /locus_tag="GWQ54_RS23435"
     CDS             complement(6295..7095)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471432.1"
                     /locus_tag="GWQ54_RS23435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_161253109.1"
                     /transl_table=11
                     /translation="MTEDEGYLLDNRQAEAGERFDALAELFDAATFRHFHALGLTAGHR
                     VWEVGAGGLSVPGGLAERVGPEGYVLATDLDTGWLTGVTPPPPVEVRRHDIGRDEAPSG
                     SFDFVHARLVLVHVTDRARALRTMAGALRPGGVLFVEDADPALQPLLCLDEYGPEQERA
                     NRLRRAFRQLLAERGADLSYGRKLPRLLRELGLERVEADAYFPVTSPAGAVLEAATVRQ
                     VRDQLVDKGLADDEDIERHLASVAAGRLDLATSPMISAWGRRPA"
     gene            complement(7115..7993)
                     /locus_tag="GWQ54_RS23440"
     CDS             complement(7115..7993)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980847.1"
                     /locus_tag="GWQ54_RS23440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_167160081.1"
                     /transl_table=11
                     /translation="MSAAPAAPQPASAEALDTAGGEKTGALDLLLVPPRARTGWRLLPA
                     RIGALCTVELQKLRHDRAELYTRAVQPALWLLIFGETFTRIKAIPTGGIPYIDYLAPGI
                     IAQSAMFIAIFYGIMIIWERDSGILTKLLVTPTPRSALITGKAFAAGVKALIQAVVVIV
                     IAALLGVALTWNPLRLLGVAVAVILGSAFFSCLSMTIAGIVLTRDRLMGIGQAITMPLF
                     FGSSALYPVSIMPGWLQAVSRANPLSYQVDALRGLLLGTPSHLALDYGVLLLAAALGIL
                     AASSLLGRLAR"
     gene            complement(7953..8807)
                     /locus_tag="GWQ54_RS23445"
     CDS             complement(7953..8807)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004029.1"
                     /locus_tag="GWQ54_RS23445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_167160078.1"
                     /transl_table=11
                     /translation="MSDDDARTGGEAVRCDGLEYAFGQTRAVDGLDLTVATGEVFGLLG
                     PNGAGKTTAIRCITTLLPVPAGRVRVFGHDAAKERMAVRRLLGYVPQQLSADSGLTGRE
                     NVALFARVFDVPRKERARRVEQALYAVDLTESADRMAKTYSGGMIRRLELAQALVSAPR
                     LLMLDEPTIGLDPIARTSVWEHINAVRRATGMTVLVTTHHMDEADQYCDRLALMHRGRI
                     RALGTPDGLKAELRERRGASGPDDPPPTLEDVFRDVAGRGLDSQGGDFRDVRSTRRTAT
                     RLG"
     gene            complement(8804..9268)
                     /locus_tag="GWQ54_RS23450"
     CDS             complement(8804..9268)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602566.1"
                     /locus_tag="GWQ54_RS23450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_161247007.1"
                     /transl_table=11
                     /translation="MAAEEPEPFDDSFADVLAAVQRLVRRRLRRGLSAPRLRGAHIELL
                     RLVATRPGIGVSAAAKELYLAGNSVSTLVNQLVKGGYMRRETDPGDRRSARLHVTPAAQ
                     ERLHDWQRRRAALMGEQVARLDPADRAALAAAMPALRRLAENLHEEAEGS"
     misc_feature    9771..9785
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.38"
                     /tool="antismash"
     misc_feature    9901..9915
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
ORIGIN
        1 accagccgaa ggtcgactgg agtacggagt tgaagttggt cagggcgctg gtctgggcgg
       61 cggccccgct gaaccctccg tcgaacggca tgatcgagaa gttgtccggg acgaagccct
      121 gggtcttggc ctcgttcagc atctgcttgc cgaaccagcc ggtgccgtcg gcggtcccgg
      181 cggtggtgac cgagacgtac aggccggggt tgttctgctg gaggatcttg gcggcgccta
      241 tctcgtgcga gatggcggcg gtgttctcgt actctggctc ttcgaggtcg aagtcgatcg
      301 ccttgagctg gtacttcgtg atgacctgct ggtacgcggc ggcggtggag gcggcgtccg
      361 agcagttctg gcccagcttg gtgccgccgt acccgccgac ggagaccgag acgtctccgc
      421 ccttggcccg gatcgagccg atgaccgcgg caaccgcggt gtcggaggag acgggggccg
      481 ttccgcccca agtcgggctg cacccacccc cgttgggggc gaggacgaag gccagctgga
      541 aggccttgag cccggtggcg tccatgatcg cggaggtgtc gggcgggttg ttgtcgaggg
      601 gcatcagata gggagcggcg gcgtaccagc ggctgccgag cccggcggcg tcggcggtcg
      661 caccggacgc ctgtcccgcg acgagggcgg tgaggccggc cgcggcgagt gcggcgctgg
      721 cggcgccgca caggagagca cgaagtggct tcatgtcgag gtctcctggg gtgggagggg
      781 ctcccagaat tgagccgtgg ccggaacacg tcaacgactt ggactagacc attgctcttc
      841 cttgaacaaa ggccgccggc cggcgaacgg agccgtgcgg gccggcgtct cccccgcggg
      901 ccccgcccca cggacagaac cgcccggccg agtgccttcc gtgtaggccg gacggcacag
      961 ccgacctgcg tgatgcccgt tctgttccta cggtggctga atggtcatca gatccggttt
     1021 ccgttccatg gcagcactca ccgccactgc cgccatcggt ctgtgctggc tcgctccgac
     1081 gcccgacagc tccgcctcct cgcccgcgca ggtcccggga gcgggccagc aggtcagtcc
     1141 ccggccgggc gcccccgctc ttccgggtga tgtgtccgcc ctgtcgtacg tggtcgcgga
     1201 cgcccgcacc ggcaaggtgc tcgccgcccg ggacgcccac cgcaagctgc cgccggccag
     1261 caccctcaag acgctgttcg ccgtcaccgc actcccccat ctgcacgccg aggaccggca
     1321 cacggtctcc gagcacgaac tggccggcat cggcccgggc agcagcctgg tgggggtcga
     1381 agccggccac acctaccagg tgtccgatct gtggcgcggc gtcttcctca gctcgggcaa
     1441 cgacgccgtg cacgtgctgg cctcgctgaa cggtggctgg gaggagaccg cggcccagat
     1501 gcaggcccag gccaggcggc tcggggccct ggacacggag gtcgtctcgc ccgacggcta
     1561 cgacaccccg ggccaggtct cctcggcgtt cgacctcgcg gtgttcggcc gggccggcct
     1621 ggcgcgcccg gacttcgccg agtactgctc gacggcgtcc gcgcagttcc ccggcggggg
     1681 cggctcgtac gggatcatga acaccaaccg gctgctctcg ggtacggacg ggatctcccg
     1741 ctacccgggg atcgtcgggg tcaagaacgg ctacacgacg aacgcgggca acacgctgat
     1801 cgcggccgcg aagcagggcg accgcacctt gctggtgacc gtcatgaacc cgcaggccgg
     1861 cggcggcttc gccgtctacg acgaggcccg caccctgctc gactggggct tcgcggcctg
     1921 ggaccacgtc gactcggtgg gctccctcga acccgttcgg gccaagtcgc cccagcggca
     1981 ggcgcagatc gcgcacgtcg cggccgtcaa gcccgaggcc gccgcggacg acgacgagcc
     2041 cggctggacg ctgacgagct cggcggccgc gggcatcgcc gtggccggaa ccctgttcct
     2101 ggtggtacgc cgccgccgcg ccgtccacgc ccgcaccgaa cggagccgcc ccacggagtg
     2161 agctcccggc ggcgtcccgg ccaccgggac gccggggcgt cgccgtggcc gcgcggttcc
     2221 ggatgggcgg ggggcggacc gcgtccgtcc gaaagacgga cccgcgcatc tcccgggcga
     2281 cggccccggg tgccccccga gggcgccccg gccggcctcc gggaggaggc tcccggggcc
     2341 cggcggccgc acaacaggat ctgaccaccc ctcaaacagg cctaagattc acagaattca
     2401 gccttccgtt gcctggaaaa cgacttcaca cctgccttcg tgcctttctt ttggccgacc
     2461 ttgaccgact cggcgacctg cacacccctc cggccgcctg caccgatgcg gcttcgttga
     2521 gtgacttcgt gtgaccgaat gtgtccggcg tggctcacgg gaccgcccgc cgcaggacag
     2581 actcctgttc gttcttcgga aaccaacccc aggagtgttc gaaatgcgga agatcgcagc
     2641 agccggcgcc gtcaccgtcg ccctactcgc caacgggctc atcggcaccg gactcggcag
     2701 cgcggcagcg gcgccggccg aggccatgaa gccgctgagc ctgtacccgc cgtcggccct
     2761 cgtcatcagc atcgagcccg gcaccggcga ccagggcgcc tccgtccagc gcgccgtcac
     2821 cctcagctgc gccccgacac cctccggcac ccaccccgca ccgggcgccg catgtgccga
     2881 actcaagggc gtacagggcg acttcaacgc cctcgtggcc agtggcgccc cacgtctgtg
     2941 tacccaggtg tggcagccgg tgaccgtgcg ggcggacggc gtctggaacg gcaagcgcgt
     3001 gagccatgtc cagacgtacg ccaactcctg cctgaaggcc gccggttccg aggccgtgtt
     3061 caacttctag gcgacccgac atcggggagg cttgcgactc cccgtctccg aagccaggtt
     3121 gaccccgtcg accgtcacac gagcaccatc tgcgaccgga ccggccgcag ggggtgacag
     3181 aaggaatcca actcgtgtta cttgacatgt cttcgtcgct tcactcgacg atgacaccgt
     3241 cctgacggag agtcaccccc cactaggagg acccgtgtcg gatccccggc ccagacgctt
     3301 cacgccccgg ccgcgcaccg ctctgctcgg cgcgctcgcc gcctgcctcg ccctcgccac
     3361 ggcgggtgcc ggcggcagcg catccgcggc ggggcccgag accctgccgc tgtccggggc
     3421 caccgtctcc gggctgcaca acacctacga tccggcggcc ttctcctatc tggcccaggc
     3481 cctggactcc ggcacctcga tgatcgagct cgacgtctgg gacgacttca tcaccaagga
     3541 gtggaaggtc agccactcca accctctggg caacagcaac aactgcgtca acgccagcac
     3601 ccccgcccag ctctacaccg gcggcgcgaa caagaacctg gagagctgcc tcgacgatgt
     3661 acgggtgtgg ctcggcgccc accccgggca cccgccgctc gtcatcaagc tggagatgaa
     3721 ggcgggcttc caggccacct acggcatgag ccccgccaag ctggaccaga cgatcagcga
     3781 ccacctgggc tcggcggtct tccgcccggc ggacctgctc gccaagcccg gcggcggaca
     3841 gtacgccacc ctcgacgagg ccgcgggcgc cggcaactgg ccgacccgac aggcactcgc
     3901 gggcaaggtg atgctcgaag tgatcccggg cacggtcgag gagggcaacc ccaccgacac
     3961 cctgtggacg gacgtcgagt acgcgcgcta cctgcgcgat ctggcggcgg ccggccgcac
     4021 cggccaggcc cagatcttcc ccgcggtcca caacgcccag gcgggcgacc cgcgttcacg
     4081 ctatgccgat gcctccatcc gcccgtggtt cgtcgtcttc gacggggacg cggcgacgta
     4141 tctgggcggc acgatcgaca ccgcctggta cgacacccat cactacttcc tcgtcatgac
     4201 cgacgcgcag aacgtcgcgc cggccctcga cgacaagaac ccctcaccgg cggacgcgca
     4261 ggcgcgggtg gcccggctcg ccgcggccca cgcgagcgtc gcctcgaacg actggacctc
     4321 ccttccccag atccagtcgc tggccctgcc gcgcggcgtg gggtagtccg gctccgccgg
     4381 acggaaaggc ggccacccgg ctcgcacgac aggagtgccg gggcggccgc ctttcgttgc
     4441 cctgcacacc cccggttccg cgtggtcgac cccgcgccgc ggccggacgg gaccggcccc
     4501 gggagacggg gagtgccacg aggtggacgc cgggccggtg ctggggcagc ggtaccggtt
     4561 ggcggagcgg ctgccgacgg gtcggcgcag gccggtggtc gaagaacgat gtccgcgaac
     4621 cgtccacgct cggggcccgg ttcaggccag cggccgaacc gtggtgcgtg tttttggaca
     4681 ggttcgacgc gtgagcacag acacatcccc cgcatcaagg caacaagatt ttcacaaact
     4741 tggcatatgc cagagagaat cttcacccat ggctcccaag tgacgcgtgt tcagcaagat
     4801 cctccttgtt gaacactgca cttcgaatcg ggacatgtcc gcatagcggc caacccccca
     4861 cctgccgctt cttgccgcac catctcgacc ggtaggcatg gaggtgcacc ggttcgccgg
     4921 gcatcccggc cgtgaccagg ccggtcgaat gctcccggcg gacccgcgcg ccaactcgcc
     4981 gcgaagcaat ggggatcgcc atgtctcaac cctcccgacc gtcccgaccc acccgccgcc
     5041 aggccatcgg cgtcaccgcc ggcaccctcg ccggactcgc cctggcgggc accgcgcacg
     5101 ccgccgtgcc cagcggccgc accgacgccc acaccggcca cgacgcgccc gaggcgttcg
     5161 acgaggtgtt ccaggggcgc cgcatacagg gcgcgccggc cgccgggcac atgcaccacg
     5221 gcggcggcta cggcgtacgg atagacggcc aggaactgca tgtgatgcag aacgccgacg
     5281 gcacctggat cagcgtcgtg aaccactacg agaccttcgc cacgccccgc gcggtcgccc
     5341 gcgccgccgt cgtcgagctc cagggcgcct ccctggtccc gctcgcctga tccggccacg
     5401 gccgccttac caccctcgtc ccacgcccaa ccaggagcac agccatggcc gttcgcaaga
     5461 accaggcaga cctcaccgcc accgagaagc gccgactcgt cgacgcgctg ctccagctga
     5521 agcgcagcgg aaggtacgac accttcgtca gcacgcacaa cgcgttcatc atgagcgaca
     5581 ccgacacggg cgaccgggtc ggccaccgtt caccgtcctt cctgccctgg caccgcagat
     5641 tcctcatcga gttcgagcag gcgctgcagt cggtcgacgc gtcggtgacc ctgccgtact
     5701 gggactggac ggtggaccgc accccgacgg cctcgctgtg ggcggccgac ttcctcggcg
     5761 gcaccggccg cagccgggac ggccaggtga tggacggccc gttcgcccag agcgcgggcg
     5821 gctggcccct caacgtccgt gtggacggcc gcacttatct gcgccgggcg ctcggcgcct
     5881 cggtcgccca gctgccgacg cgggccgagg tcgacagcgt cctcgccatg acgacgtacg
     5941 acatggcgcc gtggaacagt gcctccgacg gcttccgcaa ccacctggag ggctggcgcg
     6001 gggtcaatct gcacaaccgc gtccatgtgt gggtgggcgg ccagatggcg acgggggcct
     6061 cgcccaacga cccggtgttc tggatgcacc acgcgttcgt ggacaagctg tggtcgcagt
     6121 ggcagaccaa gcacccggcc tcgccgtatc tgccggctgc gggcacgcgg gacgtcgtgg
     6181 acctgcacga cacgatgcgg ccgtggaaca acgtgacccc ggcggacatg ctggaccaca
     6241 ggggttacta cacgtacgac accgcggcct agccgtcccg gccgccggtc gcgctcaggc
     6301 cggccggcgg ccccaggcgg agatcatcgg cgaggtcgcc aggtcgaggc ggccggcggc
     6361 gacgctcgcc agatggcgtt cgatgtcctc gtcgtcggcc aggcccttgt cgacgagctg
     6421 gtccctgacc tggcgcacgg tggccgcttc gaggacggcg ccggccggcg aggtgaccgg
     6481 gaagtacgcg tcggcctcga cccgttcgag gcccaactcc cgtagcaggc gcgggagttt
     6541 gcgcccgtag gacaggtcgg cgccgcgctc cgcgaggagt tgcctgaacg cgcggcgcag
     6601 gcggttggcc cgttcctgtt cggggccgta ctcgtcgagg cagagcagcg gctggagcgc
     6661 ggggtcggcg tcctcgacga acaggacgcc gccggggcgc agcgccccgg ccatcgtgcg
     6721 cagcgcccgg gcccggtcgg tgacgtggac caggaccagg cgcgcgtgca cgaagtcgaa
     6781 gctccccgag ggcgcctcgt cccggccgat gtcgtgccgc cgcacctcga cagggggcgg
     6841 cggggtgacg ccggtcagcc agccggtgtc gaggtccgtc gcgaggacgt atccctcggg
     6901 gccgacccgt tcggcgaggc cgcccggcac cgagagacct cccgcgccca cctcccagac
     6961 ccggtgcccc gcggtcaggc cgagggcgtg gaagtggcgg aacgtggcgg cgtcgaagag
     7021 ctcggcgagc gcgtcgaacc gctcccccgc ctcggcctgc cggttgtcca ggaggtatcc
     7081 ctcgtcctcg gtcatgggga tcatgatggc agcctcagcg ggccagcctg ccgagcagcg
     7141 aggaggccgc gaggatgccg agggccgccg cgagcagcaa caccccgtag tcgagggcga
     7201 gatgggacgg ggtgccgagc agcagtccgc gcagcgcgtc gacctggtag ctgagcgggt
     7261 tggccctgct gacggcctgg agccagcccg gcatgatcga caccgggtag agggcgctgg
     7321 agccgaagaa cagcggcatc gtgatggcct gcccgatgcc catcaggcgg tcgcgggtca
     7381 gcacgatccc ggcgatcgtc atcgacaggc aggagaagaa ggccgagccg aggatcacgg
     7441 ccacggcgac gccgagcagc cgcagcgggt tccaggtgag ggccacgccg agcagggcgg
     7501 cgatgacgat caccacgacc gcctggatga gcgccttgac gcccgccgcg aaggcctttc
     7561 cggtgatgag ggcggagcgc ggcgtcgggg tgacgaggag cttggtgagg atgcccgagt
     7621 cccgctccca gatgatcatg atgccgtaga agatggcgat gaacatggcg gactgggcga
     7681 tgatgccggg cgccagatag tcgatgtacg ggatgccacc ggtggggatc gccttgatgc
     7741 gggtgaaggt ctcaccgaag atgagcagcc acagggcggg ctgtacggcc cgggtgtaga
     7801 gctccgcgcg gtcgtgccgg agcttttgca gttcgaccgt gcacagcgcg ccgatccggg
     7861 ccggcagcag ccgccagccg gtgcgggccc gtggcgggac gagcagcaga tcgagggcgc
     7921 cggtcttctc gccccctgcg gtgtcgaggg cctcagccga ggcgggttgc ggtgcggcgg
     7981 gtgctgcgga catcgcggaa gtcacctccc tggctgtcga gcccacgccc tgcgacgtcg
     8041 cggaagacgt cctccagggt gggcggcgga tcgtcggggc cgctcgcgcc gcggcgctcg
     8101 cgcagttcgg ccttgaggcc gtcgggtgtg cccagggcgc gtatcctccc gcggtgcatg
     8161 agcgcgagac ggtcgcagta ctggtcggcc tcgtccatgt ggtgggtggt caccaggacc
     8221 gtcatgccgg tggcccggcg cacggcgttg atgtgctccc acacgctggt gcgggctatc
     8281 gggtcgaggc cgatggtcgg ctcgtcgagc atcagcaggc ggggagcgct gaccagcgcc
     8341 tgcgccagtt cgaggcggcg gatcatgccg cccgagtacg tcttggccat ccggtcggcg
     8401 gactcggtga ggtcgacggc gtacagggcc tgctcgacgc ggcgggcccg ctccttgcgg
     8461 gggacgtcga agacccgggc gaacagggcc acgttctcgc gtccggtcag gccggagtcg
     8521 gccgacagct gctgcggcac gtagccgagc agccggcgca ccgccatccg ttccttggcg
     8581 gcgtcgtgcc cgaacacccg gacgcggccc gccggtacgg gcagcagggt ggtgatgcac
     8641 cggatcgcgg tggtcttgcc cgcgccgttc gggccgagca gcccgaagac ctcgccggtg
     8701 gcgacggtca ggtcgagccc gtcgacggct ctggtctgcc cgaaggcgta ctccaagccg
     8761 tcgcaccgca ccgcctcgcc gccggtcctc gcgtcgtcgt cactcatgac ccctccgctt
     8821 cctcgtgcag gttctcggcc agccggcgca gtgcgggcat cgcggccgcc aaggcggccc
     8881 ggtcggccgg gtccaggcgt gccacctgct cgcccatgag cgcggcccgg cggcgctgcc
     8941 agtcgtgcag ccgctcctgg gctgcgggcg tcacgtggag ccgggcggag cgacggtcgc
     9001 ccggatcggt ctcgcgcctc atgtacccgc ccttcaccag ctggttgacc agcgtggaga
     9061 ccgaattgcc ggccaaatag agctccttgg cggcggccga cactccgatg ccgggccggg
     9121 tggcgaccag cctgagcagt tcgatgtggg cgccgcgcag ccggggggcg ctcagcccgc
     9181 ggcgcagcct gcgccggacg agccgctgga cggcggccag cacatcggcg aaggagtcgt
     9241 cgaacggttc gggctcttcc gcggccatac ccccattcta tctctcactc agaggtaatg
     9301 accagagggc gtcggtgccg cacaggatcg gagcgcggcc aaacgcgccg ggagtgtggc
     9361 aagcgacaca tgtggacccg ctgtgcgaac gaacgagggc cacatgacat ctttccccgg
     9421 ggccccgtgt gctccaccga acgtcattcg catcgggcag ggaaacagga gctgaacaca
     9481 cgtgccggcc atcagccaga ggatgcatct gatcctgctc tccgcatgga ccgcgctgtg
     9541 gttcgccgtg gtggagccca acggcggctt ctcgtggcac tatctgcggg ccgggggcga
     9601 gctcatcttc cagggaccct ccggcgacgg caccggcgga ctcaacctct acgcgcacca
     9661 tcccgagctc cagatgggcc ccgtcagctt cctgacggcg ggcctgttca acccgttccc
     9721 cgaacggacc ggtcagttcc tcgccgcgct cctcatgtcg gtgctcggcc tggtcatcgt
     9781 ggtcatcgcc gggcgcagtg ccgcctggta cttcctggga accggcacca accaccagcg
     9841 gctgcgacag cgcgtgctga tcgcggggct cgccttcatc ccgatgtgga tcgaggtgtc
     9901 ggtgcgcttc ggccacctcg acgacgtgct cgccctgttc ttcaccgcgc tcgcggtccg
     9961 ttcggtgacg cgcgacagcc cggtgggcac cgggatctgg ctggcgctcg cgatggactc
    10021 gaagccgacc gcggtgacgt tcgcggcgct cctcttcgcc ctgccccggc accagtggct
    10081 gcgggccggg ctgtggtgcg ctgcggtgct cgcgatcgcc tgggcgccgt tcttcctcag
    10141 caatccgcac tcgatgtcgg cggccggctt cgcgataccc aaccagcccg cgtcggcgct
    10201 gcgctggctg ggcgtcgccg acgccgagac acccggctgg gaccggcccg cgcaggccgc
    10261 gctcggactg ctcctcggcg cgatcgcggt gtggcggggc cggtgggcgg cggtggtgct
    10321 gctcggcgcc aacgcgcgga tcgtgctcga ccccagcgtc tacacctact acacggcctc
    10381 cgtcctgctc
//
